Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| coxsackie virus b4 infection of the mouse pancreas: i. detection of virus-specific rna in the pancreas by in situ hybridisation. | the pathology of coxsackie virus b4 (cvb4) infection in a murine model was investigated by in situ hybridisation using a biotinylated cdna probe derived from cvb4. during the acute phase of infection virus rna sequences were detected in the exocrine pancreas of 60% of mice infected with a pancreotropic variant of cvb4. a positive hybridisation signal was observed in other organs in some animals including the heart and liver of 1 mouse 28 days after infection. the cellular distribution of virus r ... | 1991 | 1658221 |
| [clinical and experimental study of the effect of kang er xin-i on viral myocarditis]. | kang er xin-i (kex-i) is a proved recipe used to treat viral myocarditis. it consists of lonicera japonica, ophiopogon japonicus, astragalus membranaceus mainly and possesses the effect of clearing away heat and toxic materials and supplementing the vital energy and nourishing. the clinical study was carried out with kex-i according to a random, paired and cross-over design. coenzyme q10 was used as a control and left ventricular function was observed. the result showed: after being treated with ... | 1991 | 1659497 |
| microinjected coxsackie b1 virus does not replicate in hep-2 cells. | coxsackie b1 virus did not replicate when microinjected into hep-2 cells. replication was assayed by production of infectious virus particles, synthesis of viral rna, and lysis of cells. the same virus preparation initiated replication when it was inoculated into hep-2 cells similarly to that by microinjected purified coxsackie b1 virus rna. | 1991 | 1660207 |
| coxsackievirus b4-induced development of antibodies to 64,000-mr islet autoantigen and hyperglycemia in mice. | diabetogenic coxsackievirus b4 infection produces a diabetes syndrome in susceptible mice resembling insulin-dependent diabetes mellitus. we reported a two- to threefold increased expression of a 64,000-mr (64 k) islet autoantigen in the infected mice preceding the development of hyperglycemia, suggesting a possible role of the virus in autoimmunity. to assess if the virus could be a trigger of autoimmunity, 64 k autoantibody development was correlated with hyperglycemia and virus replication in ... | 1991 | 1660313 |
| limb paralysis as a manifestation of coxsackie b virus infection. | forty-four african children (25 girls, 19 boys) are described with single or multiple limb paralysis as a manifestation of infection with coxsackie b virus. single limb paralysis involved exclusively the lower extremity, with no nervous system involvement elsewhere and a relatively benign prognosis. multiple limb paralysis occurred either alone or with other neurological deficits. only two of 15 patients followed up for five months or longer regained premorbid motor status. seven patients requir ... | 1991 | 1648520 |
| susceptibility of normal human joint tissue to viruses. | a model system has been developed to investigate the comparative ability of different viruses to replicate and persist intraarticularly. the viruses chosen for study were rubella, mumps, coxsackie b4, adenovirus and varicella zoster, a selection of viruses with different degrees of association with joint symptoms in clinical studies. our results showed that these viruses demonstrated a range of abilities to infect and persist in human joint tissue cultured in vitro. the most arthritogenic viruse ... | 1991 | 1650840 |
| [morphological study of the myocardium of mice infected with coxsackie b1 virus during the treatment with an immunomodulating drug t-activin]. | the study was undertaken to examine myocardial morphological alterations in mice treated with t-activin in different periods of viral myocarditis. two-month balb/c mice of both sexes were used in the experiments, which were intraperitoneally given 0.2 ml coxsackie b1 virus in a titer of 10(-3)-10(-7) d50 ml. the material was taken 3, 5, 7, 9, 15, and 30 days following infection. intact mice and animals given culture fluid were applied as controls. t-activin was subcutaneously injected in a dose ... | 1991 | 1664006 |
| differential effect of coxsackie b4 virus on murine pancreatic islet preproinsulin mrna and glyceraldehyde-3-phosphate dehydrogenase mrna. | 1991 | 1665434 | |
| competition binding studies with biotinylated echovirus 11 in cytofluorimetry analysis. | competition binding studies between viruses are usually performed with radiolabelled probes. in this report, a cytofluorimetric method using biotinylated echovirus (ev) 11 is described for the study of competition of enteroviruses for a common cell receptor site. an n-hydroxysuccinimide ester biotin spacer arm was used for biotinylation of csso4-purified ev 11. biotinylation did not change the infectivity of the virus (attachment to and replication in susceptible cells). with the exception of ev ... | 1991 | 1667786 |
| role of interferon in lethality and lymphoid atrophy induced by coxsackievirus b3 infection in mice. | to assess the importance of interferon (ifn) in the pathology of coxsackievirus b3 (cvb-3) infection, we evaluated both mortality rate and lymphoid involution in young adult balb/c mice infected with lethal doses of the virus and treated either with anti-ifn antibody or with murine ifn-alpha/beta. administration of antibody to ifn caused a profound worsening of the pathology and an increase in the mortality rate in infected animals. treatment with murine ifn exerted a significant ameliorative ef ... | 1991 | 1722097 |
| identification of a putative shared epitope between coxsackie virus b4 and alpha cardiac myosin heavy chain. | molecular mimicry is an important postulated mechanism for autoimmunity in viral myocarditis. the 356-1 monoclonal antibody neutralizes coxsackie virus b4 by binding to the vp1 protein and cross-reacts with mouse alpha cardiac myosin heavy chain. we used this monoclonal antibody to screen a lambda gt11 expression library made from cd-1 mouse hearts. of the 48 positive plaques/10(6) recombinant phages examined, 14 of the strongest-reacting clones were purified for additional studies. the inserts ... | 1991 | 1717188 |
| anti-coxsackievirus b3 neutralizing antibodies with pathological potential. | adolescent cd-1 mice inoculated with coxsackievirus b3 (cvb3m) will develop acute myocarditis with focal lesions by 7 days post-inoculation (p.i.). administration of murine sera containing anti-cvb3m-neutralizing antibodies into cvb3m-inoculated mice at 3 days p.i. will exacerbate myocarditis, suggesting the presence of pathological antibodies. to study potential pro-inflammatory properties of virus-induced antibodies, a panel of anti-cvb3m-neutralizing monoclonal antibodies (mabs) was generated ... | 1991 | 1717270 |
| autoanti-idiotypes exhibit mimicry of myocyte antigens in virus-induced myocarditis. | mice infected with coxsackievirus b develop immunologically mediated inflammatory myocarditis in heart tissue that results in the development of autoantibodies with multiple idiotypes. the specificity and temporal development of autoantibodies produced during coxsackievirus b3 infection were assessed. antiviral idiotypes and anti-idiotypic antibodies against coxsackievirus b3 idiotypes were detected and quantitated over 21- and 42-day periods, respectively. both polyclonal and monoclonal anti-id ... | 1991 | 1845881 |
| detection of coxsackievirus b3 rna in myocardial tissues by the polymerase chain reaction. | coxsackievirus b3 is a possible etiologic agent in some forms of myocarditis and idiopathic dilated cardiomyopathy. a method for the detection of coxsackievirus b3 rna was developed using the polymerase chain reaction based on the amplification of a cdna copy of the positive-strand viral rna. the fidelity of the method was established in two murine models for coxsackie b3 myocarditis. all cardiac specimens with adequate rna for study from coxsackie b3-infected mice contained detectable viral rna ... | 1991 | 1847008 |
| antibody to coxsackie b virus in diagnosing postviral fatigue syndrome. | to study the association between coxsackie b virus infection and the postviral fatigue syndrome and to assess the immunological abnormalities associated with the syndrome. | 1991 | 1847316 |
| selection of an attenuated coxsackievirus b3 variant, using a monoclonal antibody reactive to myocyte antigen. | previously, we described a heart-reactive monoclonal antibody (mab), 10a1, derived from a coxsackievirus b3 (cvb3)-infected mouse. this mab selectively inhibits infection of hela cells and myocytes with the myocarditic virus variant (cvb3w). a plaque-purified variant (h3) of cvb3w was isolated from the heart of an infected animal, and a second virus (h3-10a1) was obtained by growing h3 in hela cells in the presence of mab 10a1. as with the parental cvb3w virus, h3 infection of hela cells can be ... | 1991 | 1847455 |
| coxsackie b virus and postviral fatigue syndrome. | 1991 | 1849432 | |
| enteroviruses and idiopathic dilated cardiomyopathy. | previous studies have suggested that some cases of idiopathic dilated cardiomyopathy (idc) are due to persistent viral infection following an episode of viral myocarditis. viral rna sequences have recently been detected in material from patients with idc using molecular biological techniques. we tested 40 samples from recipients' hearts explanted at cardiac transplantation for the presence of enteroviral rna sequences, using a northern blotting technique. material from 19 cases of idc and 21 cas ... | 1991 | 1849988 |
| monoclonal antibody specific for the cellular receptor of echoviruses. | cell lines of primate origin carry membrane receptors which are specific for echoviruses (ev). the present report describes isolation and characterization of a monoclonal antibody (mab 143) reacting with the membrane of kb cells. the mab was selected for its protection of different cell lines from primate origin against the cpe of ev-11. this protection was found to extend to most ev serotypes and to coxsackievirus a9, while the replication of several other picornaviruses was not affected. the f ... | 1992 | 1740381 |
| combination treatment with ribavirin and interferon for coxsackievirus b3 replication. | the effects of combined treatment with ribavirin and recombinant human leukocyte interferon-alpha a/d against coxsackievirus b3 replication were investigated in cultured cells. recombinant human leukocyte interferon-alpha a/d was applied 12 hours before coxsackievirus b3 inoculation and ribavirin was applied 1 hour after coxsackievirus b3 inoculation on fl (human amnion) cell monolayers. these drugs inhibited coxsackievirus b3 replication synergistically by plaque-reduction assay. this method of ... | 1992 | 1328433 |
| extracellular matrix remodelling after coxsackievirus b3-induced murine myocarditis. | weanling inbred balb/c mice were intraperitoneally inoculated with a myocarditic variant of coxsackievirus b3. at days 1, 2, 4, 6, 8, 10, 14, 24 and 30 post-infection (p.i.), myocardial tissue was harvested for viral infectivity titrations and histological studies, including routine techniques (haematoxylin-eosin, masson trichrome and von kossa) and specialized procedures (silver impregnation for reticulin, picrosirius red stain for collagen and immunoperoxidase labelling for laminin). virus was ... | 1992 | 1329915 |
| [acute malignant myocarditis and pleurodynia as the manifestation of a probable coxsackie b viral infection]. | the authors describe a 45-year-old female patient suffering from rapid-progressing myocarditis with growing disorders in conduction, repolarization alterations and heart dilatation seen for 3 days. the patient died on the 10th day of the disease which started from fever and catarrhal phenomena. attack-like pains in the right half of the chest and abdomen, removable by narcotics, were a remarkable disease manifestation. the diagnosis of myocarditis was verified morphologically. the disease may be ... | 1992 | 1332204 |
| [using dot-immunobinding assay for the determination of coxsackie b virus specific igg in patients with viral myocarditis]. | 1992 | 1335409 | |
| demonstration of the distribution of coxsackie virus rna in neonatal mice by non-isotopic in situ hybridization. | a simple method for the demonstration of coxsackie virus rna by in situ hybridization is described. oligonucleotides complimentary to conserved sequences of coxsackie b genome were synthesised and labelled with digoxigenin using commercially available reagents. in addition to detecting all five coxsackie b strains examined, six strains of coxsackie a were also demonstrated by these probes. using one of the oligonucleotides separately it was possible to distinguish coxsackie a strains from the st ... | 1992 | 1333472 |
| [ocular histoenzymatic research on an experimental viral attack]. | we sought to investigate enzyme response appearing subsequent to sub-conjunctival administration of the coxsackie b3 virus. this virus stimulates oxidising enzymes, diaphorase and leucin aminopeptidase, dihydrofolate reductase, and adenosinetriphosphatase. the most typical enzyme changes are been in the chorion of the conjunctival mucose and the corneal parenchyma there by showing that the virus, triggers local immune defence mechanisms. the appearance of highly active langerhans cells around bo ... | 1992 | 1325834 |
| [diaphorase activity at the conjunctival level in an experimentally produced viral infection]. | by coxsackie virus type b3 subconjunctival administration it has been noticed the appearance of a antiviral defense mechanism, revealed by the presence in conjunctival chorion of lymphocytes, plasmocytes and active macrophages. it had also been studied by histochemical methods the responsive way at conjunctival level of nadh2-cytocrom-c-reductase (diaphorase) in experimentally obtained viral aggression. the injected virus has determined the stimulation of the activity of this enzyme at epithelia ... | 1992 | 1325835 |
| heat-shock protein induction in adriamycin and picornavirus-infected cardiocytes. | both chemicals, such as the chemotherapy agent adriamycin, and viruses, such as the picornaviruses coxsackievirus b3 and encephalomyocarditis virus, cause metabolic injury in myocardial cells. this injury includes depression of cellular rna and protein synthesis and production of oxygen free radicals which are known to induce increased expression of "heat-shock" or stress proteins (hsp). these hsp can stimulate potent t lymphocyte responses that may then contribute to cardiac damage associated w ... | 1992 | 1323730 |
| replication of enteroviruses in human mononuclear cells. | 1992 | 1607276 | |
| evaluation of the effects of low molecular weight heparin on inflammation and collagen deposition in chronic coxsackievirus b3-induced myocarditis in a/j mice. | coxsackievirus, group b, type 3 (cvb3) infection of a/j male mice induces chronic myocarditis with increased interstitial fibrosis and collagen deposition. heparin, a naturally occurring sulfated glycosaminoglycan, has both anti-inflammatory and antifibrotic activities besides its well-known anticoagulant activity. this study determined whether heparin treatment could decrease either cardiac inflammation or fibrosis in chronic cvb3-induced myocarditis. control mice were either untreated or treat ... | 1992 | 1321562 |
| identification of the integrin vla-2 as a receptor for echovirus 1. | cell surface receptors for echovirus, a common human pathogen, were identified with monoclonal antibodies that protected susceptible cells from infection with echovirus 1. these monoclonal antibodies, which prevented virus attachment to specific receptor sites, recognized the alpha and beta subunits of the integrin vla-2 (alpha 2 beta 1), a receptor for collagen and laminin. rd rhabdomyosarcoma cells expressed little vla-2, did not bind to 35s-labeled virus, and resisted infection until transfec ... | 1992 | 1553561 |
| mumps and coxsackie b3 virus infection of human fetal pancreatic islet-like cell clusters. | islet-like cell clusters (iccs) prepared from human fetal pancreases were infected with mumps or coxsackie b3 virus. double-labeled antibody technique showed that the viruses infected both insulin-secreting and other pancreatic cells and that secretion of immunoreactive insulin into the culture medium of the mumps virus-infected cells had already ceased on day 7. the mumps virus-infected icc clusters produced virus for 14 days, and the mumps virus antigen was detected in the iccs through the who ... | 1992 | 1322538 |
| t-lymphocyte mediated injury of beta cells in dual-aetiology diabetes mellitus in swiss albino mouse. | earlier we had described a dual aetiology diabetes mellitus (dadm) in mice injected with a sub-diabetogenic dose of streptozotocin (sd-szn) and afterwards infected with coxsackie b3 virus (cbv). further experiments were conducted to understand the mechanism of diabetogenesis. in in vitro stimulation and proliferation tests, the splenic lymphocytes (slc) of mice given either sd-szn or cbv infection showed lower responses to two t cell mitogens than those of control mice, indicating an immunosuppr ... | 1992 | 1335964 |
| high frequency of igm antibodies to coxsackie b virus in sarcoidosis patients and patients with asbestos-related lesions. | by using radioimmunoassay (ria) for detection of igm antibodies to coxsackie b viruses (cbv), the occurrence of these antibodies was investigated in patients with sarcoidosis and asbestos-related lesions. sixty-one per cent of the patients with sarcoidosis, all patients with benign asbestos pleural effusion, and 67% of those with diffuse asbestos-related pleural thickening showed cbv-igm. patients with healed sarcoidosis or pleural plaques were all negative, and among the "healthy" controls seve ... | 1992 | 1344042 |
| a mouse model of coxsackievirus myocarditis. | to develop a mouse model of coxsackievirus b3 (cvb3) myocarditis. | 1992 | 1313735 |
| polyclonal immunoglobulin therapy protects against cardiac damage in experimental coxsackievirus-induced myocarditis. | balb/c male mice infected i.p. with 2 x 10(5) plaque forming units (pfu) of coxsackievirus b3 (cvb3) develop severe myocarditis 7 days later. studies were performed to determine whether therapy with normal polyclonal immunoglobulin would prevent cardiac inflammation. partially purified immunoglobulin was derived from pooled mouse serum by ammonium sulphate precipitation. infected animals given either 100 or 1000 micrograms of this preparation for 2 days prior to infection showed greater than 50% ... | 1992 | 1349527 |
| [epidemic outbreak of viral meningitis caused by type 30 echo virus]. | the clinical records of 15 children admitted to hospital infantile de méxico federico gómez with diagnosis of viral meningitis were reviewed. they were part of 19 patients admitted with this diagnosis during a 5 week period (march 22 to april 30, 1992) and represent a significant increase of aseptic meningitis compared with the same periods of previous years at hospital infantile de mexico and in mexico city where there is an ongoing epidemic outbreak of this entity. all the patients studied had ... | 1992 | 1418647 |
| the effects of fk-506, a novel and potent immunosuppressant, upon murine coxsackievirus b3 myocarditis. | to test the therapeutic efficacy of immunosuppression with fk-506 upon coxsackievirus b3 myocarditis, c3h/he mice were inoculated with coxsackievirus b3, and the effects of fk-506 were compared to those of cyclosporine. fk-506 (2.5 mg/kg/day) or cyclosporine (25 mg/kg/day) was administered s.c. daily on days 0 to 14 (experiment i) and on days 14 to 28 (experiment ii). in experiment i, the survival rate of the fk-506 or cyclosporine-treated group was significantly lower compared with that of the ... | 1992 | 1372051 |
| acute sensory and autonomic neuropathy: possible association with coxsackie b virus infection. | this report describes a 26 year old woman with a coxsackie b virus infection complicated by an acute pandysautonomic and sensory neuropathy. electrophysiological studies suggested an axonal neuropathy. a sural nerve biopsy performed early in the disease showed axonal degeneration with a virtual absence of unmyelinated fibres and moderate loss of myelinated fibres, mainly affecting the small fibres; this differs from previous reports. an immune-mediated or direct virus action might explain the pa ... | 1992 | 1322452 |
| [possible relationship between acute viral myocarditis and dilated cardiomyopathy]. | to explore the possible relationship between acute viral myocarditis and dilated cardiomyopathy (dcm), 35 acute diffuse viral myocarditis (advm) patients with cardiac enlargement were studied for 6 years on average. the results showed that: (1) in 22 advm patients, the dilated hearts had returned to normal on x-ray films. the other 13 cases still had cardiac enlargement complicated with various degrees of cardiac insufficiency (nyha ii/iii) and ecg abnormalities. the manifestation of these 13 pa ... | 1992 | 1327706 |
| non-cytopathic infection of rhabdomyosarcoma cells by coxsackie b5 virus. | infection of rhabdomyosarcoma (rd) cells by coxsackie b5 virus (cbv5) was non-cytopathic, although low titres of infectious virus were produced after 24 h post-infection. the extent of cbv5 replication in rd cells increased after sequential passage of the virus in these cells. the rd cells from the first cycle of cbv5 infection were recovered and maintained in culture for 3 months (equivalent to 21 passages) releasing infectious virus throughout this period; these cells were considered to be per ... | 1992 | 1326261 |
| [the effect of a coxsackie viral infection on the chromosomal apparatus of the cells of fetuses and newborn mice]. | the coxsackie virus b1 has been studied for its effect on the chromosome apparatus in cells of mouse feti and newborn mice in case of intraperitoneal infection of females on the 3d week of pregnancy. it is stated that the virus readily moves across the placental barrier and induces chromosome aberrations in the cells of feti, their number being 2.5 times higher than the control level. the maximal number of structural chromosome aberrations was registered during the first three days from the mome ... | 1992 | 1333568 |
| virus infection and hand papular dermatoses in young children. | four strains of enterovirus and 1 strain of adenovirus were isolated from stool and pharyngeal swabs by inoculation into primary human embryonic kidney cell culture in 25 cases of dorsum manus infantile papular dermatitis. two strains of coxsackie a-9 virus and 1 strain of echovirus-25 were identified. with the isolated virus as antigen, a matched pair study by sera antibody test was carried out in 43 children with papular dermatitis and 40 normal individuals as controls. in 26 cases of coxsacki ... | 1992 | 1333941 |
| spectrum of monoclonal antibodies to coxsackievirus b-3 includes type- and group-specific antibodies. | fifteen monoclonal antibodies (mabs) made to coxsackievirus b-3 were tested against a panel of enteroviruses by indirect immunofluorescence. the mabs included seven which reacted with coxsackievirus b-3 only, five which reacted with more than one enterovirus included in the panel, one which reacted with broad enteroviral specificity and did not react with any heterologous virus (group specific), and two which reacted with all enteroviruses tested and at least one heterologous virus. the group-sp ... | 1992 | 1328290 |
| coxsackievirus b4 infection alters thymic, splenic, and peripheral lymphocyte repertoire preceding onset of hyperglycemia in mice. | diabetogenic coxsackievirus b4 infection may trigger autoimmune islet loss in diabetes-susceptible mice, resulting in hyperglycemia in nearly 90% of the animals at 6-8 weeks postinfection (p.i.). to ascertain whether changes in lymphocyte repertoire following infection could predispose these animals to diabetes, alterations in their thymic, splenic, and peripheral lymphocytes were analyzed. additionally, lymphocyte changes were correlated with the virus load in these tissues and with lymphocyte ... | 1992 | 1334127 |
| placental phagocytic cells infected with herpes simplex type 2 and echovirus type 19: virological and ultrastructural aspects. | placental macrophage cells were kept in a short-term culture and infected with herpes simplex type 2 virus and echovirus type 19. these were observed under optical and electron microscopy. immunofluorescence, virus titration and autoradiographic technique were used to determine if the virus was replicating in the system. the results showed that the placental phagocytic cells do not allow virus growth and that the virus particles are destroyed right after virus uptake, within 4 h post-infection. ... | 1992 | 1335148 |
| correlation between coxsackie b1 virus replication and enhanced invasiveness of shigella flexneri. | coxsackie b1 virus infection enhances the susceptibility of cultured hep-2 cells to shigella flexneri invasiveness. this can be reproduced partially with uv-inactivated virus, particularly the effect observed shortly after viral inoculation. the following phases of viral multiplication were correlated with the enhancing effect: uncoating of viral particles, synthesis of viral rna and proteins, and assembly of newly produced virus particles. uncoating of virus particles was completed within 60 mi ... | 1992 | 1314061 |
| immunoglobulin class and subclass-specific monoclonal antibody sandwich elisa for the detection of antibodies against coxsackieviruses b, types 1-5. | immunoglobulin subclass-specific elisas were developed for human igg1, igg2, igg3, igg4, igatotal, and igm directed against coxsackie b (cb) virus types 1, 2, 3, 4, and 5. in all the assays the solid phase was coated with immunoglobulin class/subclass-specific monoclonal antibodies, followed by an incubation with the serum specimens. incubation with one of the cb viruses, as well as an incubation with biotinylated serotype-specific monoclonal antibodies to the same virus type provided the virus ... | 1992 | 1321835 |
| mobilization of cutaneous immunity for systemic protection against infections. | this laboratory reported that a single subcutaneous (sc) injection of the natural steroid hormone dehydroepiandrosterone (dhea) resulted in significant protection against a lethal herpes virus type 2 encephalitis or a systemic coxsackievirus b4 infection. our previous results have shown that sc injection of dhea resulted in upregulation of the specific host immune response resulting in protection against a lethal infection. this hormone did not have any direct antiviral effects in vitro. further ... | 1992 | 1318664 |
| coxsackie b1 virus-induced changes in cell membrane-associated functions are not responsible for altered sensitivity to bacterial invasiveness. | to analyze the possible mechanisms by which coxsackie b1 virus infection affects the invasiveness of shigella flexneri, we have studied the influence of intracellular levels of na+ and k+, atpase activity, cytoplasmic membrane potential, camp level and cell communication through gap junctions. 3h after adsorption of viable or uv-inactivated coxsackie b1 virus the na(+)-k+ gradient of the cell collapsed, atpase activity decreased, the cytoplasmic membranic potential-dependent tetraphosphonium ion ... | 1992 | 1318709 |
| identification of candidate sequences that determine virulence in coxsackievirus b4. | we have previously shown that a major determinant of virulence for coxsackievirus b4 mapped to the 5' end of the viral genome. comparison of the corresponding cdna sequences of a virulent and a non-virulent virus has allowed the identification of candidate determinants of virulence in the 5' untranslated region and the capsid proteins vp1, vp2 and vp4. thirteen nucleotide substitutions were observed in a region spanning 3298 nucleotides. four mutations were detected in the non-coding region. of ... | 1992 | 1320798 |
| synergistic interaction of interferon-beta and interferon-gamma in coxsackievirus b3-infected carrier cultures of human myocardial fibroblasts. | the antiviral effects of human interferon-beta (ifn-beta) and human recombinant interferon-gamma (rifn-gamma) were studied in persistently coxsackievirus b3-infected carrier cultures of human myocardial fibroblasts over a period of 21 days. synergism was observed with concentrations as low as 30 iu of ifn-beta plus 10 iu of rifn-gamma/ml, reducing mean viral titers from 6.0 x 10(7) to 1.3 x 10(4) pfu/ml and number of infected cells from 14.4% to 0.1% as determined by quantitative in situ hybridi ... | 1992 | 1328409 |
| protective effect of a monoclonal antibody specific for an echovirus cellular receptor in human fibroblast and simian kidney cell lines. | we previously reported that infection of kb cells by echoviruses (ev) was inhibited by a kb-derived ev receptor murine monoclonal antibody (mab 143). this antibody enabled the identification of a cellular receptor common to all echoviruses (with the exception of ev-22 and -23) and coxsackievirus (cv) a9, but different from the receptor of other picornaviruses. we now present results of cell protection assays conducted with human and simian cell lines different from the kb cell line used for prod ... | 1992 | 1297174 |
| t cells expressing the gamma delta t-cell receptor potentiate coxsackievirus b3-induced myocarditis. | initial studies determined whether intraperitoneal (i.p.) injection of balb/c mice with 0.1, 1.0, and 10 mg of adriamycin (a cardiotoxic anthracycline antibiotic) for times ranging between 1 and 9 weeks prior to i.p. injection of 10(5) pfu of coxsackievirus b3 (cvb3) would alter the severity of virus-induced myocarditis. prior adriamycin exposure enhanced pathogenicity of a poorly pathogenic cvb3 variant (h310a1) but had no effect on myocarditis produced by the pathogenic variant (h3). cardiac v ... | 1992 | 1328680 |
| interferon treatment reduced adherence, invasiveness and intracellular multiplication of shigella flexneri in coxsackie b1 virus-infected cells. | the effect of interferon treatment on interaction of shigella flexneri with in vitro cultured cells was investigated. pretreatment of hep-2 cells with human interferons had no effect on the susceptibility of cells to s. flexneri, measured by invasiveness and adhesiveness. human leukocyte interferon and human recombinant interferon-alpha-a reduced adhesiveness, intracellular multiplication and invasiveness of s. flexneri in hep-2 cells preinfected with coxsackie b1 virus. also non-receptor mediat ... | 1992 | 1329437 |
| ongoing enterovirus-induced myocarditis is associated with persistent heart muscle infection: quantitative analysis of virus replication, tissue damage, and inflammation. | coxsackievirus b3-induced myocarditis in different immunocompetent mouse strains was used as a model to investigate interrelationships between virus replication and development of chronic enteroviral heart disease. using in situ hybridization to detect enteroviral rna, we show that heart muscle infection is not only detected in acute myocarditis but is also detected during the chronic phase of the disease. coxsackievirus b3 could evade immunological surveillance in a host-dependent fashion, thus ... | 1992 | 1309611 |
| enteroviral infection of mice with severe combined immunodeficiency. evidence for direct viral pathogenesis of myocardial injury. | inbred mice with genetically determined severe combined immunodeficiency (scid) lack mature t and b lymphocyte functions. to distinguish direct viral effects in the pathogenesis of myocarditis from those mediated by antigen-specific and histocompatibility-complex-restricted host immunity, we inoculated coxsackievirus b3 into homozygous young adult scid mice. we found that infected scid mice invariably developed extensive myocarditis between 7 and 14 days postinoculation with high subsequent mort ... | 1992 | 1309927 |
| cytotoxic and viral neutralizing antibodies crossreact with streptococcal m protein, enteroviruses, and human cardiac myosin. | the development of autoimmunity in certain instances is related to infectious agents. in this report, cytotoxic monoclonal antibodies (mabs) that recognize epitopes on both enteroviruses and the bacterium streptococcus pyogenes are described. murine anti-streptococcal mabs that were crossreactive with streptococcal m protein, human cardiac myosin, and other alpha-helical coiled-coil molecules were found to neutralize coxsackieviruses b3 and b4 or poliovirus type 1. the viral-neutralizing anti-st ... | 1992 | 1311095 |
| crystallization and preliminary x-ray diffraction studies of coxsackievirus b1. | preparations of coxsackievirus b1 (cvb1) derived from an infectious cdna clone have been crystallized in multiple crystal forms. using high intensity synchrotron radiation, an orthorhombic form of the crystals was shown to diffract x-rays to at least 2.9 a resolution. the unit cell has a primitive lattice with dimensions a = 323 a, b = 450 a, and c = 522 a. a crystallographic asymmetric unit of these cvb1 crystals probably contains an entire virus particle, implying the presence of 60-fold non-c ... | 1992 | 1311388 |
| focal ventricular thinning caused by indomethacin in the late phase of coxsackievirus b4 murine myocarditis. | indomethacin has been shown to increase virus titers and to worsen cardiac injury in the acute phase of coxsackievirus b4 murine myocarditis. the authors evaluated the effects of indomethacin on the histopathologic changes in a later phase of this disease after virus clearance. two-day old cd1 mice were infected with coxsackievirus b4. ten days later, surviving animals were randomized to receive indomethacin or saline intraperitoneally for 10 days. they were then euthanatized, and their hearts w ... | 1992 | 1311498 |
| mumps virus infects beta cells in human fetal islet cell cultures upregulating the expression of hla class i molecules. | the ability of mumps virus to infect pancreatic beta cells and cause alterations in their hla expression was evaluated in cultured human fetal islet cell clusters. mumps virus could be isolated during the whole culture period (6-8 days) and 60% of cells, including beta cells, contained viral nucleocapsid protein at the end of the culturing. a minor decrease in insulin secretion was observed in some of the infected cultures. the infection was invariably associated with an increase in the expressi ... | 1992 | 1311693 |
| viral myocarditis--a tale of two diseases. | 1992 | 1309925 | |
| the possible role of coxsackie a and echo viruses in the pathogenesis of type i diabetes mellitus studied by igm analysis. | igm antibodies to coxsackie b virus (cbv) have recently been observed in the serum of a relatively high proportion of children with newly diagnosed insulin-dependent diabetes mellitus (iddm). in the present study, 108 iddm patients below the age of 15 years, diagnosed during the period 1976 to 1985, were investigated at the onset of their disease by mu-antibody-capture radioimmunoassay (ria) of igm against seven different enterovirus antigens, namely virions of cbv serotypes 1-5 (cbv 1-5) and pr ... | 1992 | 1312561 |
| viral genomic detection in the hearts of c3h/he mice with experimental coxsackievirus b3 myocarditis by gene amplification using the polymerase chain reaction. | on the basis of the detection of the enteroviral rna in the hearts of patients with healed myocarditis and dilated cardiomyopathy, we investigated cardiac viral persistence in experimental myocarditis. weanling c3h/he mice were given myocarditis by inoculation with coxsackievirus b3 (nancy strain), and their hearts were examined by genomic studies and viral isolation up to the 180th day after inoculation. the virus was isolated from the heart until the 9th day. by slot-blot hybridization, viral ... | 1992 | 1312649 |
| effects of polyethylene glycol conjugated superoxide dismutase on coxsackievirus b3 myocarditis in mice. | the aim was to examine the role of oxygen derived free radicals in the development of myocarditis by investigating the effects of polyethylene glycol conjugated superoxide dismutase (peg-sod), a potent scavenger of oxygen free radicals, upon coxsackievirus b3 (cb3) myocarditis. | 1992 | 1336713 |
| detection of viral rna in experimental coxsackievirus b3 myocarditis of mice using the polymerase chain reaction. | the presence of the viral rna in the myocardium in experimental coxsackievirus b3 myocarditis of mice was investigated using the polymerase chain reaction (pcr). four-week-old c3h/he mice (n = 35) were inoculated with coxsackievirus b3 (nancy strain, 10(5) plaque-forming units/mouse). we used a pair of primers, which encompass a part of the 5' end sequence of the coxsackievirus b3 genome and can also detect many enteroviral rnas. we found that hearts were positive for the viral rna from 2 to 21 ... | 1992 | 1337265 |
| effect of verapamil on acute coxsackievirus b3 murine myocarditis. | the effect of verapamil (ver) on cvb3 murine myocarditis was investigated. it was found that ver could aggravate the myocardial inflammation, increase the viral replication in myocardium, and raise mortality in mice with viral myocarditis when the drug was injected within the first 6 days after the cvb3 inoculation. | 1992 | 1337875 |
| coxsackie b virus igm in children at onset of type 1 (insulin-dependent) diabetes mellitus: evidence for igm induction by a recent or current infection. | thirty-five children with newly-diagnosed type 1 (insulin-dependent) diabetes mellitus and their 47 siblings were investigated for the presence of igm antibodies to coxsackie b virus serotypes 1-5 (cbv 1-5) with the aid of mu-antibody-capture radioimmunoassays. when a high cut-off value was used, 16 (46%) diabetic children and 16 (34%) siblings showed cbv-igm. of the siblings of diabetic patients positive for cbv-igm, 11 (44%) were cbv-igm-positive; the corresponding figure for the siblings of n ... | 1992 | 1314203 |
| lipopolysaccharide suppresses cytokine release from coxsackie virus-infected human monocytes. | infections by coxsackie virus b3 (cvb3) have been reported to be associated with an enhanced influx of mononuclear leukocytes into afflicted tissue. current evidence indicates that monocytes/macrophages are specifically involved in cvb3-induced myocarditis by maintaining a chronic inflammatory response. to examine susceptibility and reactivity to cvb3, freshly isolated human monocytes were exposed to various virus doses (0.1-10 moi) in the presence or absence of macrophage-activating lipopolysac ... | 1992 | 1314406 |
| effect of enterovirus infection on susceptibility of hela cells to shigella flexneri invasivity. | invasiveness of shigella flexneri m90t in hela cells was significantly increased when cells were preinfected with poliovirus 1, coxsackievirus b3 and echovirus 6. this effect was dependent on the dose of virus used, evident at early stages of viral infection and lasted hours before the appearance of a cytopathic effect. an increase of bacterial invasion ability was also noticed when hela cells were incubated with uv-inactivated enteroviruses. this enhancing effect obtained with both viable and u ... | 1992 | 1364020 |
| a method for the preparation of purified antigens of coxsackievirus b3 from a large volume of cell culture supernatant. | a simple procedure was used for the concentration and partial purification of coxsackievirus b3 (nancy strain). for a large-scale production of virus. vero cells grown in roller bottles were used. virus concentrate from a large volume of cell culture supernatant was prepared by precipitation with 6% (w/w) polyethylene glycol. this crude antigen was further purified by banding in cesium chloride gradient using ultracentrifugation. the infectivity and haemagglutination activity of virus were check ... | 1992 | 1364026 |
| detection of enteroviruses in cell cultures by using in situ transcription. | in situ transcription (ist) was shown to be useful for the detection of human enteroviral rna in cultured cells. a primer to detect a wide variety of enteroviral genomes and a coxsackievirus type b3 genome-specific primer were demonstrated to be efficient in ist assays. transcription times greater than 10 to 30 min did not significantly improve the acquisition of a specific signal, whereas the signal-to-noise ratio decreased with time. inclusion of actinomycin d to suppress dna-dependent dna pol ... | 1992 | 1370849 |
| persistent rubella-specific igm reactivity in the absence of recent primary rubella and rubella reinfection. | between two and seven sera from cases of persistent detection of rubella-specific igm for periods in excess of 2.5 months, but in the absence of recent primary rubella or rubella reinfection, were examined for rheumatoid factor, heterophile antibody, and igm reactivity against toxoplasma and a number of viruses. the relative avidity of the rubella-specific igg1 has been assessed in all the sera by two methods. none of the sera contained rheumatoid factor or heterophile antibody, nor did any cont ... | 1992 | 1314284 |
| increased frequency of coxsackie b virus igm in women with spontaneous abortion. | igm antibodies to coxsackie b virus serotypes 1-5 (cbv 1-5) were studied by radioimmunoassay (ria) of blood samples from women who had undergone a spontaneous abortion or given birth to a stillborn child. results were compared with those of controls comprising healthy pregnant women who had not suffered such experiences. among 48 women with abortions, 16 (33%) had cbv-igm, while among the controls only three of 37 (8%) had these antibodies, a statistically significant difference (p less than 0.0 ... | 1992 | 1314861 |
| disturbance of mouse pancreatic beta-cell function following echo 4 virus infection. | 1992 | 1486985 | |
| finding out how a viral hitchhiker snags a ride. | 1992 | 1553554 | |
| successive infection of coxsackievirus b3 and encephalomyocarditis virus: an animal model of chronic myocarditis. | successive infection of coxsackievirus b3 and encephalomyocarditis virus was investigated as a disease model of chronic myocarditis. four-week-old c3h/he mice were inoculated with coxsackievirus b3 and then inoculated with encephalomyocarditis virus at 8 weeks old. the hearts were evaluated on histopathological changes compared with those of non-infected mice and mice infected with either virus alone. at 10 weeks old, the hearts of the mice infected successively with both viruses showed co-exist ... | 1992 | 1325552 |
| a 44,000 glycoprotein is involved in the attachment of echovirus-11 onto susceptible cells. | cellular receptors play an important role in viral pathogenesis. until now little was known on echovirus (ev) receptor. using detergent-treated kb cell extracts as immunogen, a mouse monoclonal antibody (mab 143) was produced that selectively blocks the attachment of ev-11 to kb and other susceptible cells. by immunoblotting, mab 143 detected a 44,000 protein on susceptible cell lines but not on cell lines from nonprimate origin. the receptor protein complex, purified from kb cell membranes by i ... | 1992 | 1604819 |
| mapping of the rd phenotype of the nancy strain of coxsackievirus b3. | the rd variants of group b coxsackieviruses differ from their parental strains in having the ability to replicate in a human rhabdomyosarcoma cell line, rd. the nucleotide sequence of the p1 region of the rd variant of coxsackievirus b3 strain nancy (cb3nrd) was determined by sequencing cloned cdnas, obtained by pcr amplification. a comparison between the established nucleotide sequence and that of the p1 region from the parental virus revealed 12 point mutations which corresponded to six amino ... | 1992 | 1326828 |
| detection of coxsackie b virus myocarditis on tc-99m pyp myocardial imaging. | 1992 | 1312000 | |
| coxsackievirus b3-induced production of tumor necrosis factor-alpha, il-1 beta, and il-6 in human monocytes. | infections by coxsackievirus b3 (cvb3) have previously been shown to cause acute and chronic myocarditis characterized by a heavy mononuclear leukocyte infiltration and myocyte necrosis. because clinical and experimental evidence suggested that cardiac damage may result from immunologic rather than viral mechanisms, we examined in this study the in vitro interaction of cvb3 with human monocytes. cvb3 was capable of infecting freshly harvested monocytes as revealed by immunofluorescence and relea ... | 1992 | 1312105 |
| search for coxsackievirus b3 rna in idiopathic dilated cardiomyopathy using gene amplification by polymerase chain reaction. | a polymerase chain reaction (pcr) amplification assay was developed to detect coxsackievirus b3 ribonucleic acid (rna) in blood and myocardial tissue of explanted hearts from 40 patients who underwent cardiac transplantation and in 1 normal heart. twenty-one patients were affected by idiopathic dilated cardiomyopathy of different duration and 19 by coronary artery disease. coxsackievirus b3 in vitro infected vero cells and cells infected by related human enteroviruses (coxsackievirus b2, b4, and ... | 1992 | 1311139 |
| association of heat shock protein 70 with enterovirus capsid precursor p1 in infected human cells. | members of the human heat shock (hsp) family of related proteins are involved in the intracellular folding, transport, and assembly of proteins and protein complexes. we have observed that human heat shock protein 70 (hsp70) is associated with the capsid precursor p1 of poliovirus and coxsackievirus b1 in infected hela cells. antiserum generated against hsp70 coimmunoprecipitated the poliovirus protein p1, an intermediate in capsid assembly. similarly, alpha-virion serum coimmunoprecipitated hsp ... | 1992 | 1310763 |
| t lymphocyte responses in cvb3-induced murine myocarditis. | three monoclonal antibodies (mab) to group a streptococcus m5 serotype (mab 36.2.2, 49.8.9 and 54.2.8) cross-reactivity bind to various heart antigens (including myosin, tropomyosin and vimentin) and neutralize a myocarditic variant of coxsackievirus b-3 (nancy) (cvb3). the existence of shared antigenic epitopes between the two distinct infectious agents and the heart implies that antigenic mimicry may form the foundation of the autoimmune response. plaque purified variants of cvb3 were isolated ... | 1993 | 7685923 |
| mapping of antigenic determinants of the adenine-nucleotide translocator and coxsackie b3 virus with synthetic peptides: use for the diagnosis of viral heart disease. | 1993 | 7689426 | |
| [the correction of a secondary immunological deficit caused by the coxsackie b3 virus by using an immunomodulator of bacterial origin]. | 1993 | 8079587 | |
| differential behaviour in pancreas and heart of two coxsackievirus b3 variants. | in order to characterize viral kinetics and pathogenic properties of two intratypic variants of coxsackievirus b type 3, balb/c mice were intraperitoneally inoculated and serial samples harvested from days 2 to 28. although both cb3o (amyocarditic) and cb3m (myocarditic) variants induced similar early infectivity titres in pancreas, only the latter led to severe acinar necrosis, followed in turn by patent viraemia and subsequent focal myocarditis. nevertheless, when both variants were inoculated ... | 1993 | 8150596 |
| bleomycin differentially inhibits picornavirus, herpesvirus and poxvirus replication in a human carcinoma cell line. | ms-757, a cervix carcinoma cell line, was exposed to bleomycin at concentrations up to 200 microm for periods of 1 to 24 hours. bleomycin treatment caused the level of dna synthesis in uninfected cells to drop to 13% of the level achieved in the controls. protein synthesis fell to 50%, but rna synthesis was not affected. exposure of uninfected cells to 200 microm bleomycin for 24 h did not induce significant cell death measured as permeability to trypan blue). a tetrazolium dye-reduction assay, ... | 1993 | 7514894 |
| [the clinical diagnosis and treatment of meningitis caused by coxsackie b viruses]. | clinical features of meningitis caused by coxsackie virus b were analyzed in 369 adult patients. the authors draw attention to polymorphism of manifestation and diagnostic signs of the diseases. reaferon used in complex treatment was established to be the most effective. the drug considerably shortened period of fever and drove away main clinical symptoms. | 1993 | 7518170 |
| stabilization of the antigenicity of coxsackie b procapsids by disoxaril. | disoxaril added to cell cultures infected with coxsackie b virus serotypes 3 or 4 increased the yield and stabilized the antigenicity of the procapsids. in the absence of disoxaril, the procapsids lost their broad antigenicity after a short period of time. prevention of this loss by disoxaril was observed in radioimmunoassays of igm in sera from patients infected with various enteroviruses. the procapsids are useful in serological diagnosis of enterovirus infections, and the addition of disoxari ... | 1993 | 8113348 |
| the integrin vla-2 binds echovirus 1 and extracellular matrix ligands by different mechanisms. | the integrin vla-2 mediates cell adhesion to collagen and laminin and also functions as a virus receptor, mediating cell surface attachment and infection by a human pathogen, echovirus 1. to determine whether extracellular matrix proteins and virus interact with vla-2 in the same manner, we carried out a detailed comparison of these two functions and found that they differed markedly in six different respects. in contrast to the ecm/vla-2 interaction, echovirus 1 binding did not discriminate bet ... | 1993 | 7686920 |
| [standardization of an inhibition elisa technique for the serologic diagnosis of coxsackie virus group b infections]. | an inhibition elisa technic to detect antibodies aimed at coxsackie b viruses was standardized. the method was type-specific since it could detect antibodies to 4 coxsackie b serotypes (it wasn't tried on serotypes b2 and b4 because the strains were not available). the comparison with the neutralization technic showed 85% coincidence, 91% sensitivity and 82% specificity. all the reactives used in the assay were produced in our laboratory. | 1993 | 7800894 |
| effect of astragalus membranaceus on electrophysiological activities of acute experimental coxsackie b-3 viral myocarditis in mice. | a murine model for observing the effect of astragalus membranaceus (am) on electrophysiological action of the right ventricular myocardium was developed in 4-week-old male balb/c mice infected with coxsackie b-3 virus (cb3v). the conventional microelectrode technique and a real-time data processor system were used. the survival rate in am-treated mice was much higher and the percentage of abnormal action potential was much lower than those in infected control mice (p < 0.05, p < 0.01, respective ... | 1993 | 8032064 |
| coxsackie virus infections in rheumatic fever. | the close relationship between group a beta hemolytic streptococci (gabs) and rheumatic fever is a well established one. however, the concept of the streptococcus as the sole etiologic agent of the rheumatic heart disease (rhd) has been challenged over the past years. since coxsackievirus group b (cvb) has long been proposed as a cause of acquired valvular disease simulating rheumatic fever, we attempted in this study to document infections with this group of viruses in patients with rheumatic f ... | 1993 | 8244506 |
| enterovirus infections in neonates. a retrospective study of 21 cases. | the aim of this study was to define the major features of enterovirus infections in the neonatal period based on our own experience. | 1993 | 8261072 |
| [viral infections--clinical pictures and laboratory diagnosis. part 2: coxsackie b virus, echovirus, enteroviruses, ebv, fsme virus and yellow fever virus]. | 1993 | 8294077 | |
| increased transcription of interleukin-6 in the brains of mice with chronic enterovirus infection. | an animal model of chronic enteroviral infection was established by using pcr to detect viral genomes in animal tissues and to compare levels of transcription of a variety of cytokines in the brain. chronic coxsackie-virus b1 infection was found in both brain and skeletal muscle of mice infected as neonates. the viral infection cleared by 240 days post-infection. elevated levels of tumour necrosis factor alpha and interleukin-6 (il-6) would appear to be linked to acute and chronic infection resp ... | 1993 | 8385699 |
| enhancement of coxsackievirus b3 myocarditis in mice by lobenzarit disodium through inhibition of splenic pan t cells. | the aim was to test the efficacy of the immune system modulator lobenzarit disodium in the treatment of coxsackievirus b3 myocarditis. | 1993 | 8386064 |
| ultrastructural study of cell injury induced by coxsackievirus b3 in pancreatic and cardiac tissues. | balb/c weanling mice were intraperitoneally inoculated with a myocarditic variant of coxsackievirus b3, with the aim of characterizing more thoroughly the features of virus-induced cell injury in pancreas and heart, as well as to compare ultrastructural alterations with histological and virological findings. during the first week post-infection (pi), all animals developed acinar pancreatitis, followed by focal myocarditis. at electron microscopy, acinar cells showed patent distortion, including ... | 1993 | 8201911 |
| coxsackie b virus infections in women with miscarriage. | to study the possible role of coxsackie b virus serotypes 1-5 (cbv 1-5) as an etiological factor in miscarriage, 97 women with miscarriage were tested for the presence of cbv-igm by radioimmunoassays (rias) and compared with 113 control women undergoing voluntary interruption of pregnancy in the same gestational week. of the 80 women with miscarriage before the 13th week of gestation, 34 (42%) had cbv-igm, whereas 18 of 100 (18%) corresponding control women had these antibodies, a statistically ... | 1993 | 8388026 |
| echovirus type 19 and herpes simplex type 2 infection in human placenta tissue explants. | 1. light and electron microscopy have been used to characterize echovirus 19 and herpes simplex type 2 infection of human placenta tissue in vitro. immunofluorescence, autoradiography and virus adsorption were used to determine virus replication in this system. 2. placental tissue was permissive to echovirus 19. trophoblast cells were lysed with liberation of mature virions. however, during the 48-h period of observation, few cells were damaged and the trophoblastic structure was maintained. 3. ... | 1993 | 8268819 |