Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| expression of nitric oxide synthase isoforms in different stages of buffalo (bubalus bubalis) ovarian follicles: effect of nitric oxide on in vitro development of preantral follicle. | the present study was designed to investigate the expression of nitric oxide synthase (nos) isoforms in buffalo ovarian preantral (pfs), antral (afs) and ovulatory (ofs) follicles (experiment 1); effect of no on in vitro survival and growth of pfs (experiment 2) and nos activity in immature oocytes by nadph-diaphorase test (experiment 3). in experiment 1, nos isoforms (neuronal, inducible and endothelial) were localized immunohistochemically; mrna and protein expression was analyzed by semi-quan ... | 2011 | 21924465 |
| translocation of active mitochondria during buffalo (bubalus bubalis) oocytes in vitro maturation, fertilization and preimplantation embryo development. | mitochondria are energy-supplying organelles, whose distribution and functional integrity are necessary for cell survival and development. in this study, the mitochondrial distribution pattern and activity during buffalo oocyte in vitro maturation, fertilization and preimplantation embryo development were revealed using a fluorescent dye and confocal laser scanning microscopy. distribution of active mitochondria changed during buffalo oocyte in vitro maturation. active mitochondria were transfer ... | 2011 | 21950622 |
| Buffalo (Bubalus bubalis) Epiphyseal Proteins Counteract Arsenic-Induced Oxidative Stress in Brain, Heart, and Liver of Female Rats. | Arsenic (As) toxicity through induction of oxidative stress is a well-known mechanism of organ toxicity. To address this problem, buffalo epiphyseal proteins (BEP, at 100 µg/kg BW, i.p. for 28 days) were administered intraperitoneally to female Wistar rats exposed to As (100 ppm sodium arsenite via drinking water for 28 days). Arsenic exposure resulted in marked elevation in lipid peroxidation in brain, cardiac, and hepatic tissues, whereas significant (p?<?0.05) adverse change in catalase, supe ... | 2011 | 22095291 |
| Comparative Quality Assessment of Buffalo (Bubalus bubalis) Semen Chilled (5°C) in Egg Yolk- and Soya Milk-Based Extenders. | Egg yolk-Tris is most commonly used semen extender; however, its use involves hygienic risk, interference with fertility and poor microscopic examination. Therefore, replacement of egg yolk with a plant-based component with protective effects on spermatozoa would be advantageous. In present study, we observed effect of soya milk-based extenders on dilution and liquid preservation of Murrah buffalo bull semen at 5°C up to 72 h in comparison with conventional egg yolk-Tris extender (Ext.1). In exp ... | 2011 | 22017209 |
| a comparative study on mimosine, 3,4-dihydroxy pyridone (3,4-dhp) and 2,3-dihydroxy pyridone (2,3-dhp), purine derivatives (pd) excretion in the urine, thyroid hormone and blood metabolites profiles of thai swamp buffalo (bubalus bubalis) and murrah buffalo (bubalus bubalis). | four thai swamp buffaloes (sb) and four murrah buffaloes (mb) fed a based diet of fresh ruzi grass (bachiaria ruziziensis) with an increased proportion of fresh leucaena leaves. intake of nutrients in animals increased when ruzi grass was mixed with leucaena. digestibility of nutrients were the highest in sb and mb fed diets containing 25% and 50% of leucaena, respectively, and nitrogen (n) balances in both animal breeds were varied among diets. the regression equation coefficient of mimosine + ... | 2011 | 21928129 |
| methyl coenzyme m reductase (mcra) gene based phylogenetic analysis of methanogens population in murrah buffaloes (bubalus bubalis). | the aim of the present study was to decipher the diversity of methanogens in rumen of murrah buffaloes so that effective strategies can be made in order to mitigate methane emission from these methanogens. in the present study diversity of rumen methanogens in murrah buffaloes (bubalus bubalis) from north india was evaluated by using mcr-a gene library obtained from the pooled pcr product from four animals and by using mega4 software. a total of 104 clones were examined, revealing 26 different m ... | 2011 | 21887637 |
| derivation, characterization and differentiation of buffalo (bubalus bubalis) amniotic fluid derived stem cells. | amniotic fluid cells (afcs) are obtained from amnion for pre-natal analysis and can be cultured in vitro. heterogeneous amniotic fluid (af) contains various cell types, and it is believed that some of these cells possess the stem cell properties. the aim of this study was to characterize these cells by phenotypical and genotypical means in buffalo. the differentiation potential of amniotic fluid stem (afs) cells was carried out by converting these cells into neurons. the afcs were cultured witho ... | 2011 | 22107067 |
| Molecular mining of alleles in water buffalo Bubalus bubalis and characterization of the TSPY1 and COL6A1 genes. | Minisatellites are an integral part of eukaryotic genomes and show variation in the complexity of their organization. Besides their presence in non-coding regions, a small fraction of them are part of the transcriptome, possibly participating in gene regulation, expression and silencing. We studied the minisatellite (TGG)(n) tagged transcriptome in the water buffalo Bubalus bubalis across various tissues and the spermatozoa, and characterized the genes TSPY1 and COL6A1 discovered in the process. | 2011 | 21949806 |
| baccharis megapotamica var. weirii poisoning in water buffalo (bubalus bubalis). | an outbreak of an acute disease in buffalo (bubalus bubalis) caused by the ingestion of baccharis megapotamica var. weirii occurred in the southern region of brazil. ten out of 50 buffalo died 24-48 hr after being introduced into a pasture containing abundant amounts of the plant. factors influencing the ingestion of the plant and consequent toxicosis included hunger, stress caused by shipment, and unfamiliarity with the plant. clinical signs included serous ocular discharge, incoordination, mil ... | 2011 | 21908301 |
| Isolation, culturing and characterization of feeder-independent amniotic fluid stem cells in buffalo (Bubalus bubalis). | Heterogeneous amniotic fluid contains various cell types. The aim of this study was to characterize and differentiate some of the key stemness attributes of the amniotic fluid-derived cells in buffalo (Bubalus bubalis). The amniotic fluid (AF) cells were cultured without feeder cells, in DMEM containing 15% FBS, 1% non-essential amino acids, 1% penicillin/streptomycin/ampicillin, 1% vitamin solution, and 1% l-glutamine in 5% CO(2) in humidified air at 38.5±0.5°C. After 6days of culture different ... | 2011 | 21982462 |
| molecular modeling and docking characterization of dectin-1 (pamp) receptor of bubalus bubalis. | dectin-1, is a type ii transmembrane receptor protein which contains a single extracellular ctld (c-type lectin domain), stalk, transmembrane domain and an itam (immunoreceptor tyrosine-based activation motifs) in its cytoplasmic tail. dectin-1 has the ability to recognize fungal β-glucans, which are carbohydrate pamps found predominantly in fungal cell walls. the recognition of fungal β-glucans by dectin-1 helps in a variety of cellular responses, like host protection, such as fungal uptake and ... | 2011 | 22015804 |
| comparative proteomics to evaluate multi drug resistance in escherichia coli. | drug resistance in food-borne bacterial pathogens is an almost inevitable consequence of the use of antimicrobial drugs, used either therapeutically or to avoid infections in food-producing animals. in the past decades, the spread and inappropriate use of antibiotics have caused a considerable increase of antibiotics to which bacteria have developed resistance and, moreover, bacteria are becoming resistant to more than one antibiotic simultaneously. understanding mechanisms at the molecular leve ... | 2011 | 22120138 |
| characterization of prlr and ppargc1a genes in buffalo (bubalus bubalis). | more than 40 million households in india depend at least partially on livestock production. buffaloes are one of the major milk producers in india. the prolactin receptor (prlr) gene and peroxisome proliferators activated receptor-γ coactivator 1-alpha (ppargc1a) gene are reportedly associated with milk protein and milk fat yields in bos taurus. in this study, we sequenced the prlr and ppargc1a genes in the water buffalo bubalus bubalis. the prlr and ppargc1a genes coded for 581 and 819 amino ac ... | 2011 | 22215963 |
| experimental inoculation of neospora caninum in pregnant water buffalo. | the aim of this study was to characterize the pathogenesis of neospora caninum in experimentally inoculated pregnant water buffalo (bubalus bubalis). twelve mediterranean female water buffaloes ranging in age from 4 to 14 years old and seronegative to n. caninum by indirect fluorescent antibody test (ifat) were involved. ten females were intravenously inoculated with 10(8) tachyzoites of nc-1 strain at 70 (n=3) or 90 (n=7) days of pregnancy (dp). two control animals were inoculated with placebo ... | 2011 | 22244534 |
| natural trematode infection in liver of water buffalo (bubalus bubalis): histopathological investigation. | this study reports the infection of liver and bile ducts, carried out from november 2008 to april 2010, on 730 randomly selected water buffaloes-bubalusbubalis, infected with the amphistome trematode parasite explanatum explanatum (creplin, 1847) fukui, 1929. macroscopic examination revealed massive infection of adult fluke in bile ducts and intrahepatic ductules in 131 (18%) cases. the predominant features were multifocal granulomatous nodules throughout the luminal surface of the bile ducts. h ... | 2011 | 22654314 |
| effects of plane of nutrition on slaughtering traits and meat characteristics in murrah graded male buffalo (bubalus bubalis) calves in nepal. | an experiment was conducted using 17 male buffalo calves to assess the effects of plane of nutrition on slaughtering traits and meat characteristics. to attain 250 kg body weight (bw), the calves were allocated into three groups: high (h), low-high (l-h) and low (l) corresponding to concentrate levels receiving the concentrate at 1.50% of bw, 0.75% of bw until 190 kg bw and 1.50% thereafter, and 0.75% of bw, respectively. the animals had ad libitum access to urea-treated rice straw. no significa ... | 2011 | 22574796 |
| mitochondrial dna diversity patterns in pakistani buffalo. | the indian subcontinent is considered to be the likely centre of river buffalo domestication, based on population dynamics, archaeological evidence and genetic diversity. recent studies on mitochondrial dna diversity have drawn useful conclusions about the domestication history of bubalus bubalis. the conclusions of these studies are, however, incomplete, unless samples can also be analysed from pakistan, which contains the second largest buffalo population of the world. here, we report the resu ... | 2011 | 22486503 |
| effect of incorporation of additives in tris-based egg yolk extender on buffalo (bubalus bubalis) sperm tyrosine phosphorylation during cryopreservation. | phosphorylation of tyrosine residues on sperm protein is a known indicator of capacitation and a major intracellular signalling event. there is evidence that sperm cryopreservation promotes tyrosine phosphorylation and is associated with reduced fertility of spermatozoa. under this study, cryoprotective role of different additives namely taurine, trehalose, catalase and 4-bromophenacyl bromide on buffalo sperm quality was evaluated. buffalo semen was cryopreserved in tris-based egg yolk extender ... | 2011 | 22364363 |
| phenotype characterization of staphylococcus species strains isolated from buffalo (bubalus bubalis) milk. | the objective of the current study was to isolate and identify phenotypes of staphylococcus spp. strains derived from buffalo (bubalus bubalis) milk. a total of 548 milk samples from 137 buffalo were cultured in columbia agar enriched with 5% defibrinated sheep blood. determination of the capacity of staphylococcus aureus to produce enterotoxins a-d and toxic shock syndrome toxin-1 (tsst-1) was achieved by reverse passive latex agglutination (rpla). antimicrobial sensitivity of s. aureus strains ... | 2011 | 22362803 |
| soya-lecithin in extender improves the freezability and fertility of buffalo (bubalus bubalis) bull spermatozoa. | egg yolk is routinely used as a cryoprotectant in semen extenders. however, it may contain cryoprotective antagonists, and there are hygienic risks associated with its use. proteins of plant origin, like soya-lecithin, lack these hazards. the aim of this study was to use soya-lecithin as a cryoprotectant in extender and to investigate its effects on in vitro quality and in vivo fertility of buffalo semen. semen from three buffalo bulls was frozen in tris-citric extender containing 5.0%, 10% or 1 ... | 2011 | 22211278 |
| characterization and in silico analysis of pregnancy-associated glycoprotein-1 gene of buffalo (bubalus bubalis). | pregnancy-associated glycoproteins (pags) are trophoblastic proteins belonging to the aspartic proteinase family secreted by different placental cells of many mammalian species. they play a pivotal role in placentogenesis, foetomaternal unit remodeling, and implantation. the identification of the genes encoding those proteins will be helpful to unravel the intricate embryogenomic functions during pregnancy establishment. considering importance of these proteins, the present study was undertaken ... | 2011 | 22567354 |
| effect of selenium, zinc, and copper supplementation on blood metabolic profile in male buffalo (bubalus bubalis) calves. | twenty male buffalo calves (15 months, 200.2 ± 9.75) were divided into four groups of five animals in each and fed diets without (t1) or supplemented with 0.3 ppm selenium (se) + 40 ppm zinc (zn) (t2), 0.3 ppm se + 40 ppm zn + 10 ppm copper (cu) (t3), and 40 ppm zn + 10 ppm cu (t4) for 120 days, during which blood samples were collected on days 0, 40, 80, and 120. concentrations of glucose, total protein, albumin, globulin, urea, uric acid, and creatinine were similar in all the four groups. the ... | 2011 | 21947859 |
| cloning and sequencing of indian water buffalo (bubalus bubalis) interleukin-3 cdna. | full-length cdna (435 bp) of the interleukin-3(il-3) gene of the indian water buffalo was amplified by reverse transcriptase-polymerase chain reaction and sequenced. this sequence had 96% nucleotide identity and 92% amino acid identity with bovine il-3. there are 10 amino acid substitutions in buffalo compared with that of bovine. the amino acid sequence of buffalo il-3 also showed very high identity with that of other ruminants, indicating functional cross-reactivity. structural homology modell ... | 2011 | 22152058 |
| a rapid method for authentication of buffalo (bubalus bubalis) meat by alkaline lysis method of dna extraction and species specific polymerase chain reaction. | buffalo (bubalus bubalis) meat is a major item of export from india but export of beef i.e. meat from cattle (bos indicus) is prohibited. also, adulteration of buffalo meat with that of beef (meat from cattle) is a common fraudulent practice because of prohibition on cow slaughter in most states of india. food analysts require precise identification techniques to implement such regulations. in the present study, a method of dna extraction by alkaline lysis from meat samples and speciation of buf ... | 2011 | 24425899 |
| immunohistochemical studies of the epididymal duct in egyptian water buffalo (bubalus bubalis). | using immunohistochemistry (ihc), this study aimed to evaluate the regional distribution pattern of some biologically active proteins in the epididymis of egyptian water buffalo and to determine the structural-functional relationships of the different epididymal structures. wax-embedded sections from different regions of the epididymal duct from adult, clinically healthy, buffalo bulls were used. primary antibodies against angiotensin converting enzyme (ace), s-100, galactosyltransferase (galtas ... | 2011 | 19836061 |
| quantification, morphology and ultrastructure of preantral follicles of buffalo (bubalus bubalis) foetuses. | the objective of this study was to determine the number, morphology and ultrastructure of preantral ovarian follicles of buffalo (bubalus bubalis) foetuses at different ages. quantification revealed number of primordial, primary and secondary follicles of 48,857 ± 17,506, 26,000 ± 20,452, 18,428 ± 10,875 and 18,375 ± 19,690, 225 ± 349, 326 ± 288 at 12-34 cm and 35-60 cm crown rump length (crl), respectively. follicular diameter values were 28.9 (± 3.4), 34.7 (± 5.9) and 59.4 (± 12.6) μm; oocyte ... | 2011 | 20403125 |
| localization and expression of follicle-stimulating hormone receptor gene in buffalo (bubalus bubalis) pre-antral follicles. | follicle-stimulating hormone (fsh) stimulates antral follicles to grow, but its role in earlier stages (pre-antral) of follicle development, if any, is obscure. aim of this study was to study the expression of follicle-stimulating hormone receptor (fshr) gene in different sizes of pre-antral follicles (pfs) (<150, 200, 250, 300, 350, 400 μm) and to find out an optimum dose of fsh for better growth, development and steroidogenesis of pfs in vitro. buffalo ovaries were collected from a local abatt ... | 2011 | 20403128 |
| analysis of active components of rhinoceros, water buffalo and yak horns using two-dimensional electrophoresis and ethnopharmacological evaluation. | cornu rhinoceri asiatici (rhinoceros horn, rh), cornu bubali (water buffalo horn, wbh), and cornu bovis grunniens (yak horn, yh) are traditional chinese medicine (tcm), and have been used in china for thousands of years. in this study, ethnopharmacological experiments were used to evaluate and verify the traditional efficacies of horns. area under curve (auc) was used to quantify the pharmacological efficacy strength of three horns. two-dimensional electrophoresis (2-de) was used to analyze the ... | 2011 | 21268260 |
| extended cytogenetic maps of sheep chromosome 1 and their cattle and river buffalo homoeologues: comparison with the oar1 rh map and human chromosomes 2, 3, 21 and 1q. | cytogenetic maps are useful tools for several applications, such as the physical anchoring of linkage and rh maps or genome sequence contigs to specific chromosome regions or the analysis of chromosome rearrangements. recently, a detailed rh map was reported in oar1. in the present study, we selected 38 markers equally distributed in this rh map for identification of ovine genomic dna clones within the ovine bac library chori-243 using the virtual sheep genome browser and performed fish mapping ... | 2011 | 21282943 |
| production of interspecies handmade cloned embryos by nuclear transfer of cattle, goat and rat fibroblasts to buffalo (bubalus bubalis) oocytes. | the possibility of producing interspecies handmade cloned (ihmc) embryos by nuclear transfer from donor cells of cattle, goat and rat using buffalo oocytes as recipient cytoplasts was explored. zona-free buffalo oocytes were enucleated by protrusion cone-guided bisection with a microblade. after electrofusion with somatic cells, reconstructed oocytes were activated by calcimycin a23187, treated with 6-dimethylaminopurine and were cultured in k-rvcl-50® medium for 8 days. although the cleavage ra ... | 2011 | 21288667 |
| long term effect of ovum pick-up in buffalo species. | the aim of this study was to evaluate the effect of an ovum pick-up (opu) treatment carried out for 9 months in buffalo (bubalus bubalis) species. eight pluriparous non-lactating buffalo cows underwent opu for 9 months. recovered cumulus enclosed oocytes (cocs) were classified and cocs suitable for in vitro embryo production (ivep) were in vitro matured (ivm), fertilized (ivf) and cultured (ivc) to the blastocyst (bl) stage. animals were monitored for a total period of 270 days, but at the summe ... | 2011 | 21306842 |
| dual promoter lentiviral vector generates transgenic mice expressing e2-csfv glycoprotein in their milk, but impairs early identification of transgenic embryos. | lentiviral vectors containing the green fluorescent protein gene have been successfully used to select transgenic embryos before transfer to a surrogate mother. however, there are apparently no reports regarding early detection of transgenic embryos using a lentiviral vector carrying an additional transcription unit for tissue-specific expression of a valuable protein. in this study, two hiv-based lentiviral vectors were constructed. the first one contained the green fluorescent protein (gfp) co ... | 2011 | 21316751 |
| assessment of buffalo (bubalus bubalis) sperm dna fragmentation using a sperm chromatin dispersion test. | chromosomal fragmentations or damage in sperm dna has considerable value in determination of semen quality. however, rapid and/or simple method to assess sperm dna integrity in buffalo has apparently not been reported. in the present study, scd was used for the first time in buffalo bulls for assessment of sperm dna fragmentation. a modified scd protocol, under bright field microscope was developed and validated by comparison with other routine tests which can be used for processing of samples. ... | 2011 | 21382103 |
| biochemical and hormonal composition of follicular cysts in water buffalo (bubalus bubalis). | the objective of this study was to examine the follicular fluid biochemical and hormonal changes associated with ovarian follicular cysts in buffalo. follicular fluid was aspirated from eight cysts and eight preovulatory follicles, and subjected to biochemical and hormonal analyses. cysts were characterized by a greater (p<0.01) concentration of nitric oxide and lesser concentrations of ascorbic acid and glucose than that of preovulatory follicles (p<0.01 and p<0.05, respectively). furthermore, ... | 2011 | 21392901 |
| standardisation of an indirect enzyme-linked immunosorbent assay for the detection of brucella antibodies in milk from water buffalo (bubalus bubalis) in italy. | an indirect enzyme-linked immunosorbent assay (elisa) was evaluated for the detection of brucella antibodies in milk from water buffalo (bubalus bubalis linnaeus, 1758). the test accuracy was evaluated on milk samples from the campania region in italy. a total of 100 negative samples were collected from 10 officially brucellosis-free herds in salerno province, while 30 positive samples were collected from 3 herds in caserta province, where animals gave positive results to the official tests and ... | 2011 | 21240852 |
| effects of heavy metals and pesticides on buffalo (bubalus bubalis) spermatozoa functions in vitro. | industrial toxic metals, pollutants and bio-accumulative pesticides interfere with the male reproductive functions in farm animals. frozen-thawed semen samples were incubated with heavy metals (cadmium and lead) and pesticides (chlorpyrifos and endosulfan) of different concentrations (0, 0.005, 0.05, 0.02, 0.1, 0.5, 1.0, 2.0 and 4.0 μg/ml) for 1 h, and various spermatozoa functional parameters and in vitro fertilization rates were assessed. any significant effect was assessed by comparing the 1 ... | 2011 | 21241381 |
| free amino acids profile and quantities of 'sirt', 'bohca' and 'sekerpare' pastirma, dry cured meat products. | pastirma is a dry-cured and semi-fermented meat product, produced from whole beef or water buffalo muscles. sixteen to twenty different types of pastirma can be produced from a carcass, and each pastirma type has a different name, shape and quality characteristics. the objective of this study was to determine the free amino acid (faa) composition, ph and moisture values of 'sirt', 'bohca' and 'sekerpare' pastirma. | 2011 | 21254071 |
| green laser irradiation effects on buffalo semen. | the overall objective of this paper is to develop a more sensitive and less costly technique of laser irradiation of spermatozoa at certain wavelengths and exposure times suitable for improvement of buffalo semen quality. a 532 nm continuous wave (cw) dpss laser light has been used to irradiate buffalo semen for different time intervals. three semen pools from three different bulls (bubalus bubalis) were used in the experiment, each pool was divided into six groups : control (not irradiated), an ... | 2011 | 21220155 |
| early pregnancy detection of iraqi riverine buffalo (bubalus bubalis) using the biopryn enzyme-linked immunosorbent assay for pspb and the progesterone assay. | this study was undertaken to detect pregnancy in iraqi riverine buffalo (bubalus bubalis) using three different methods (rectal palpation, plasma progesterone concentration and detection of the presence of pregnancy-specific protein b (pspb) with the biopryn(®) enzyme-linked immunosorbent assay (elisa) test. the aim of the study was to identify the most sensitive, early and accurate method for detecting pregnancy. twenty-two female riverine buffalo that were 6.0 ± 0.93 years old were used. four ... | 2011 | 20825581 |
| activation of zona-free buffalo (bubalus bubalis) oocytes by chemical or electrical stimulation, and subsequent parthenogenetic embryo development. | parthenogenetic activation using zona-free oocytes offers an alternative model that could be applied to develop protocols for the activation of reconstructed embryos for cloning. the aim of this study was to compare the efficacy of different methods for the activation of zona-free buffalo oocytes in terms of their effects on the developmental competence of parthenogenetic embryos. the effects of zona removal on parthenogenetic activation and in vitro developmental competence of metaphase ii oocy ... | 2011 | 20825585 |
| the microanatomy of the palatine tonsils of the buffalo (bos bubalus). | the palatine tonsils play a key role in initiating immune responses against antigens entering the body through the mouth. they are also replication sites of some pathogens. there is no data available about the structure of the palatine tonsils of the egyptian water buffalo. therefore, palatine tonsils of 14 clinically healthy buffalo bulls (2-3 years old) were examined macroscopically and microscopically using light, and transmission electron microscopes. the tonsils had an elongated kidney shap ... | 2011 | 20833436 |
| freeze-thaw induced genotoxicity in buffalo (bubalus bubalis) spermatozoa in relation to total antioxidant status. | use of cryopreserved semen has become an important tool in assisted reproduction but freezing and thawing cause sub-lethal damage to spermatozoa. this is detrimental to sperm because of the membrane damage including permeability and integrity. an excess generation of reactive oxygen species (ros) creates oxidative stress due to reduced antioxidant status of the cryopreserved spermatozoa. in the present study fresh buffalo semen was collected and divided into two aliquots. one aliquot was used fo ... | 2011 | 20853151 |
| microvasculature of the buffalo (bubalus bubalis) choroid plexuses: structural, histochemical, and immunocytochemical study. | the choroid plexuses (cps) in mammals produce the cerebrospinal fluid (csf). in the literature, the morphology of cps and the process that regulates the production of csf are virtually nonexistent for domestic ruminants. thus this study has two aims: 1. to investigate the morpho-structure of the buffalo cp microvasculature utilizing light microscopy (lm), scanning electron microscopy (sem) and transmission electron microscopy (tem) techniques, and 2. to investigate the relationship between the b ... | 2011 | 21181712 |
| a rare case of centric fission and fusion in a river buffalo (bubalus bubalis, 2n = 50) cow with reduced fertility. | a 5-year-old river buffalo cow underwent cytogenetic investigation since it had only one male offspring, apparently with normal body constitution, which died one month after birth. the female carrier had normal body conformation and internal sex adducts, as revealed by rectal palpation performed by a specialist veterinary practitioner. the cow was found to carry a complex and rare chromosome abnormality. indeed, a centric fission of one river buffalo (bbu) chromosome 1 with a subsequent (or simu ... | 2011 | 20693780 |
| identification, characterization and expression analysis of hepcidin gene in sheep. | hepcidin is part of the innate immune system, and it plays a central role in the regulation of iron homeostasis. this peptide has been previously characterized in man, non-human primates, rat, mouse, dog, swine, cattle, horse, fishes, reptiles and birds but until now not in sheep. the aim of this study was to sequence, characterize and perform hepcidin expression analysis in different tissues collected from healthy sheep. the resulting open reading frame consisted of 249bp predicted to encode an ... | 2011 | 20723953 |
| effects of chemical activation treatment on development of swamp buffalo (bubalus bubalis) oocytes matured in vitro and fertilized by intracytoplasmic sperm injection. | the objective of this study was to optimize the activation protocol for buffalo oocytes after intracytoplasmic sperm injection (icsi). the release of the second polar body (pb) at 3, 6 and 9 h after icsi of in-vitro matured oocytes activated either with 5 μm ionomycin (io) or with 7% ethanol (etoh) was preliminary examined. the highest rate of second pb extrusion occurred at 3 h of activation, and the second pb extrusion in etoh group was significantly higher than that in io group. oocytes that ... | 2011 | 20546174 |
| follicular dynamics and oestrous detection in thai postpartum swamp buffaloes (bubalus bubalis). | this study characterized follicular activity and oestrous behaviour from 5 to 9 days post-calving up to the 4th ovulation postpartum (pp) in 16 multiparous (range 2-7 parities) thai swamp buffalo cows (bubalus bubalis), aged 4-12 years and weighing from 432 to 676 kg. ovarian follicular activity was examined by transrectal ultrasonography (tus) every morning. oestrous detection was performed twice daily by direct personal observation of behaviour and for presence of clear cervical mucus discharg ... | 2011 | 20546176 |
| detection of follicular apoptosis in water buffalo (bubalus bubalis) ovary by histology and nick end labelling technique. | the objective of this experiment was to assess the features and extent of follicular apoptosis in the water buffalo (bubalus bubalis) ovary using classical histology and nick end labelling technique. ovaries (n=40) procured from the slaughterhouse were used for the study. the sections (5 μm) were used for detection of terminal deoxynucleotidyl transferase-mediated dutp-biotin nick end labelling (tunel) and classical histology (h&e). those follicles showing ≥ 5% tunel positivity (tunel assay) and ... | 2011 | 20070580 |
| in vitro evaluation of liquid-stored buffalo semen at 5°c diluted in soya lecithin based extender (bioxcell®), tris-citric egg yolk, skim milk and egg yolk-citrate extenders. | this study was designed to compare the quality of liquid-stored buffalo bull spermatozoa in soya lecithin based extender bioxcell(®) (biox), milk (milk), tris-citric egg yolk (tey) and egg yolk-citrate (eyc) extender at 5°c. semen was collected from five nili-ravi buffalo (bubalus bubalis) bulls of 6-7 years of age with artificial vagina over a period of 3 weeks (two consecutive ejaculates once in a week). semen ejaculates having more than 60% motility were pooled, split into four aliquots, dilu ... | 2011 | 20070582 |
| effect of pineal proteins at different dose level on fluoride-induced changes in plasma biochemicals and blood antioxidants enzymes in rats. | pineal glands secrets melatonin and various proteins and peptides which has many physiological functions. in keeping with this view, present experiment was conducted to know the effect of buffalo (bubalus bubalis) pineal proteins (pp) at different dose level on fluoride-induced changes in plasma biochemicals and blood antioxidants enzymes in female rats. for this, we took 30 adult female wistar rats (133-145 g body weights, bw) and divided into five groups (control, group i; 150 ppm fluoride (f) ... | 2011 | 20509005 |
| buffalo (bubalus bubalis) embryonic stem cell-like cells and preimplantation embryos exhibit comparable expression of pluripotency-related antigens. | in this study, inner cell mass (icm) cells were isolated from in vitro produced buffalo blastocysts and were cultured on mitomycin-c treated buffalo foetal fibroblast feeder layer for producing embryonic stem (es) cells. among different sources (hatched vs expanded blastocysts) or methods (enzymatic vs mechanical), mechanical isolation of icm from hatched blastocysts resulted in the highest primary colony formation rate and the maximum passage number up to which es cells survived. putative es ce ... | 2011 | 20042025 |
| inferring the phylogeny of bovidae using mitochondrial dna sequences: resolving power of individual genes relative to complete genomes. | molecular techniques that assess biodiversity through the analysis of a small segment of mitochondrial genome have been getting wide attention for inferring the mammalian diversity. due to their highly conserved nature, specific mitochondrial genes offer a promising tool for phylogenetic analysis. however, there is no established criteria for selecting the typical mitochondrial dna (mtdna) segments to achieve a greater resolving power. we therefore chose the family bovidae as a model and compare ... | 2012 | 22399841 |
| molecular characterization of mhc-drb cdna in water buffalo (bubalus bubalis). | in the present study, water buffalo mhc (bubu)-drb cdna was cloned and characterized. the 1022 base long-amplified cdna product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. the bubu-drb sequence showed maximum homology with the bola-drb3*0101 allele of cattle. a total of seven amino acid residues were found to be unique for the bubu-drb sequence. the majority of amino acid substitutions was observed in the β(1) domain. residues associated with important fu ... | 2012 | 22481880 |
| gastrointestinal parasitic infection in diverse species of domestic ruminants inhabiting tribal rural areas of southern rajasthan, india. | a total of 415 adult domesticated ruminants, 130 cattle (bos taurus), 108 buffaloes (bubalus bubalis), 94 goats (capra hircus) and 83 sheep (ovis aries) inhabiting tribal rural areas of southern rajasthan, india were investigated for evidence of gastrointestinal protozoan and helminthic infections. in southern rajasthan humid ecosystem is predominant and has number of perennial freshwater bodies. fresh faecal samples of these animals were examined microscopically by direct wet smear with saline ... | 2012 | 24431582 |
| the correlation between age, body weight and testicular parameters in murrah buffalo bulls raised in brazil. | buffalo are an economically important source for meat and milk production, especially in brazil. however, important aspects of their biology remain unknown thus far. herein, we describe the reproductive characteristics of male murrah buffalo (bubalus bubalis) raised under extensive management conditions by applying biometrics associated with testicular weight. we analyzed seven males, divided into two groups: g1, which consisted of four 18-month-old animals, and g2, which consisted of three 24-m ... | 2012 | 22986925 |
| a nonsense mutation in the tyrosinase gene causes albinism in water buffalo. | oculocutaneous albinism (oca) is an autosomal recessive hereditary pigmentation disorder affecting humans and several other animal species. oculocutaneous albinism was studied in a herd of murrah buffalo to determine the clinical presentation and genetic basis of albinism in this species. | 2012 | 22817390 |
| molecular differentiation of peroxysome proliferator activated receptor coactivator-1 among different breeds of bubalus bubalis. | peroxysome proliferator activated receptor coactivator-1 gene (ppargc1a) is a positional and functional candidate gene for milk fat yield. it has key role in energy, fat and glucose metabolism. single nucleotide polymorphisms (snps) in exon-8 of ppargc1a are reported to be associated with milk fat yield in dairy cattle. in the present investigation ppargc1a was partially amplified (around 767bp) by designing gene specific primer and confirm by sequencing the amplicon and its comparison with the ... | 2012 | 22829739 |
| tissue-specific temporal exome capture revealed muscle-specific genes and snps in indian buffalo (bubalus bubalis). | whole genome sequencing of buffalo is yet to be completed, and in the near future it may not be possible to identify an exome (coding region of genome) through bioinformatics for designing probes to capture it. in the present study, we employed in solution hybridization to sequence tissue specific temporal exomes (tst exome) in buffalo. we utilized cdna prepared from buffalo muscle tissue as a probe to capture tst exomes from the buffalo genome. this resulted in a prominent reduction of repeat s ... | 2012 | 22768984 |
| nucleotide sequencing and snp detection of toll-like receptor-4 gene in murrah buffalo (bubalus bubalis). | toll-like receptor-4 (tlr-4) has an important pattern recognition receptor that recognizes endotoxins associated with gram negative bacterial infections. the present investigation was carried out to study nucleotide sequencing and snp detection by pcr-rflp analysis of the tlr-4 gene in murrah buffalo. genomic dna was isolated from 102 lactating murrah buffalo from ndri herd. the amplified pcr fragments of tlr-4 comprised of exon 1, exon 2, exon 3.1, and exon 3.2 were examined to rflp. pcr produc ... | 2012 | 27398236 |
| comparison of worm development and host immune responses in natural hosts of schistosoma japonicum, yellow cattle and water buffalo. | yellow cattle and water buffalo are two of the most important natural hosts for schistosoma japonicum in china. previous observation has revealed that yellow cattle are more suited to the development of s. japonicum than water buffalo. understanding more about the molecular mechanisms involved in worm development, as well as the pathological and immunological differences between yellow cattle and water buffalo post infection with s japonicum will provide useful information for the vaccine design ... | 2012 | 22414188 |
| differential expression of th1- and th2- type cytokines in peripheral blood mononuclear cells of murrah buffalo (bubalus bubalis) on tlr2 induction by b. subtilis peptidoglycan. | peripheral blood mononuclear cells (pbmcs) discriminate microbial pathogens and induce t-cell responses of appropriate effector phenotype accordingly. toll-like receptors (tlrs), in part, mediate this microbial recognition and differentiation while the development of t-cell effector functions critically depends on the release of th1- or th2- type cytokines. in the present study, buffalo pbmcs were stimulated under in vitro culture conditions by bacillus subtilis cell wall petidoglycan, a tlr2 li ... | 2012 | 25049659 |
| detection of anti-borrelia burgdorferi antibodies in buffaloes (bubalus bubalis) in the state of pará, brazil. | this study aimed to investigate the frequency of homologous antibodies of igg class against borrelia burgdorferi in buffaloes in the state of pará, brazil. blood serum samples from 491 buffaloes were analyzed by means of the indirect elisa test, using crude antigen produced from a cultivar of the north american strain g39/40 of b. burgdorferi. there were 412 positive samples (83.91%), and there was no statistically significant difference in the proportions of positive animals between the 81.69% ... | 2012 | 23070455 |
| isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (bubalus bubalis) in argentina. | parainfluenza virus type 3 (piv3) was isolated from dairy buffaloes (bubalus bubalis) naturally affected with respiratory and reproductive clinical conditions. | 2012 | 22716217 |
| serological and bacteriological responses of water buffalo (bubalus bubalis) vaccinated with two doses of brucella abortus strain rb51 vaccine. | thirty-two water buffalo (bubalus bubalis) calves aged 6–10 months were used to evaluate serological responses to brucella abortus strain rb51 (rb51) vaccination in a dose-response study and to compare the use of two selective media for the isolation of rb51. the animals were randomly divided into three treatment groups. groups i-iii received the recommended vaccine dose (rd) twice 4 weeks apart, rd twice 18 weeks apart and saline once, respectively. lymph nodes were excised from the three group ... | 2012 | 22297422 |
| serological profile of buffalo (bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose bengal, 2-mercaptoethanol and complement fixation tests. | the serological profiles of 21 female buffaloes vaccinated between 3 and 8 months of age using brucella abortus strain 19 (s19) were evaluated by rose bengal (rbt), 2-mercaptoethanol (2me) and complement fixation (cft) tests. the serum strains were collected in day zero, 15, 30, 45, 60th days and subsequently to each 30 months, until 720th day after vaccination. no animal showed reaction in day zero. in 15th day above 95% of animals revealed reaction in all tests. all the animals presented absen ... | 2012 | 22284623 |
| isolation of a gene encoding a cellulolytic enzyme from swamp buffalo rumen metagenomes and its cloning and expression in escherichia coli. | ruminants are capable of hydrolyzing lignocellulosic residues to absorbable sugars by virtue of the microbial communities residing in their rumen. however, large sections of such microbial communities are not yet culturable using conventional laboratory techniques. therefore in the present study, the metagenomic dna of swamp buffalo (bubalus bubalis) rumen contents was explored using culture-independent techniques. the consensus regions of glycosyl hydrolase 5 (gh5) family of cellulases were use ... | 2012 | 23134306 |
| molecular cloning, sequence characterization and heterologous expression of buffalo (bubalus bubalis) oviduct-specific glycoprotein in e. coli. | oviductin is a high molecular weight oviduct-specific glycoprotein secreted by the non-ciliated epithelial cells of oviduct during estrous cycle and early pregnancy. it plays an important role during fertilization and early embryonic development. the oviductin gene from oviductal tissues of buffalo was successfully cloned and sequenced. the sequence analysis revealed that buffalo and cattle oviductin share very high homology between their cdna sequences. the predicted amino acid sequences of the ... | 2012 | 22782592 |
| chlamydiaceae in riverine buffalo (bubalus bubalis) and cows (bos taurus) in egypt with and without signs of reproductive disease. | to obtain information and compare the prevalence of chlamydiaceae in riverine buffalo (bubalus bubalis) and cows (bos taurus) in egypt with and without clinical signs of reproductive disease. | 2012 | 22712774 |
| diversity of salmonella spp. serovars isolated from the intestines of water buffalo calves with gastroenteritis. | salmonellosis in water buffalo (bubalus bubalis) calves is a widespread disease characterized by severe gastrointestinal lesions, profuse diarrhea and severe dehydration, occasionally exhibiting a systemic course. several salmonella serovars seem to be able to infect water buffalo, but salmonella isolates collected from this animal species have been poorly characterized. in the present study, the prevalence of salmonella spp. in water buffalo calves affected by lethal gastroenteritis was assesse ... | 2012 | 23098237 |
| utilization of elisa using thioredoxin peroxidase-1 and tandem repeat proteins for diagnosis of schistosoma japonicum infection among water buffaloes. | the presence of animal reservoirs in schistosoma japonicum infection has been a major obstacle in the control of schistosomiasis. previous studies have proven that the inclusion of control measures on animal reservoir hosts for schistosomiasis contributed to the decrease of human cases. animal surveillance should therefore be included to strengthen and improve the capabilities of current serological tests. | 2012 | 22953018 |
| clinical and pathological insights into johne's disease in buffaloes. | alternative diagnostic tools and interesting epidemiological assumptions were associated with an outbreak of johne's disease. in a buffalo herd infected with paratuberculosis, seven clinically affected animals and 21 animals with anti-mycobacterium avium elisa reactions were identified. total herd included 203 buffaloes. most lesions were comparable to those described in buffaloes and cattle affected by johne's disease. water buffalo behaviors such as communal nursing and allosuckling may be add ... | 2012 | 22528538 |
| acp5 (uteroferrin): phylogeny of an ancient and conserved gene expressed in the endometrium of mammals. | type 5 acid phosphatase (acp5; also known as tartrate-resistant acid phosphatase or uteroferrin) is a metalloprotein secreted by the endometrial glandular epithelium of pigs, mares, sheep, and water buffalo. in this paper, we describe the phylogenetic distribution of endometrial expression of acp5 and demonstrate that endometrial expression arose early in evolution (i.e., before divergence of prototherian and therian mammals ~166 million years ago). to determine expression of acp5 in the pregnan ... | 2012 | 22278982 |
| a complementary diagnosis of naturally occurring tuberculosis in water buffaloes (bubalus bubalis) in rio de janeiro using a mpb70-elisa, brazil. | as tuberculosis is still a worldwide infection and buffalo breeding represents an important economic activity in various countries, the purpose of this study was to employ an enzyme-linked immunosorbent assay (elisa) using mpb70 as a capture antigen for the diagnosis of naturally occurring tuberculosis in water buffaloes in brazil. after the introduction of newly acquired cattle onto a tuberculosis (tb) free farm, an outbreak of tb was recorded in a mixed herd comprising water buffaloes (21) and ... | 2012 | 23274697 |
| effect of season, late embryonic mortality and progesterone production on pregnancy rates in pluriparous buffaloes (bubalus bubalis) after artificial insemination with sexed semen. | the use of sexed semen technology in buffaloes is nowadays becoming more and more accepted by farmers, to overcome the burden of unwanted male calves with related costs and to more efficiently improve production and genetic gain. the aim of this study was to verify the coupling of some variables on the efficiency of pregnancy outcome after deposition of sexed semen through ai. pluriparous buffaloes from two different farms (n = 152) were screened, selected, and subjected to ovsynch protocol for ... | 2012 | 23265928 |
| impact of gonadotropin supplementation on the expression of germ cell marker genes (mater, zar1, gdf9, and bmp15) during in vitro maturation of buffalo (bubalus bubalis) oocyte. | the present study was designed to investigate whether gonadotropins [follicle-stimulating hormone (fsh) and luteinizing hormone (lh)] and buffalo follicular fluid (bff) supplementation in maturation medium influences the transcript abundance of germ cell marker genes [maternal antigen that embryos require (mater), zygote arrest 1 (zar1), growth differentiation factor 9 (gdf9), and bone morphogenetic protein 15 (bmp15)] mrna in buffalo (bubalus bubalis) oocytes. buffalo ovaries were collected fro ... | 2012 | 23263936 |
| pharmacokinetics and relative bioavailability of praziquantel in healthy water buffalo after oral and intramuscular administration. | 2012 | 22239339 | |
| effects of plane of nutrition on growth, feed intake, digestibility and nitrogen balance in murrah graded male buffalo (bubalus bubalis) calves in nepal. | an experiment was conducted using 17 male buffalo calves to assess the effects of plane of nutrition on dry matter intake (dmi), daily gain (dg), body size measurement, apparent digestibility and nitrogen (n) balance. to attain 250 kg bw, the calves were allocated into three groups: h, l-h and l, receiving the concentrate at 1.50% of bw, 0.75% of bw until 190 kg bw and 1.50% thereafter and 0.75% of bw, respectively. the animals had ad libitum access to urea-treated rice straw (utrs). the dmi of ... | 2012 | 22250739 |
| characterization of pou5f1 (oct4) gene and its promoter in buffalo esc-like cells identifies multiple transcription start sites and expression of four pseudogenes. | in the present study, we cloned and characterized the buffalo (bubalus bubalis) oct4 ortholog expressed in embryonic stem cell (esc) like cells and its promoter region. the 5'- and 3'-race experiments were conducted to analyze the transcription initiation site and regulatory regions. the comparative analysis of buffalo oct4 promoter with other mammalian orthologs revealed high conservation. among the regulatory regions highest similarity was observed between buffalo, bovine and sheep. interestin ... | 2012 | 22019428 |
| effect of scriptaid, a histone deacetylase inhibitor, on the developmental competence of handmade cloned buffalo (bubalus bubalis) embryos. | the aim of the present study was to investigate the effect of scriptaid, a histone deacetylase inhibitor, on the developmental competence of handmade cloned (hmc) and parthenogenetically activated (pa) buffalo embryos. scriptaid treatment was given for 10 h immediately after reconstruction of hmc and pa embryos. treatment of hmc embryos with 500 or 1000 nmol/l scriptaid significantly increased (p<0.01) the cleavage rate (91.3±2.8 and 91.9±2.0%, respectively) than that of controls (80.7±2.4%). bl ... | 2012 | 21924478 |
| Ovum pick-up and in vitro embryo production (OPU-IVEP) in Mediterranean Italian buffalo performed in different seasons. | This study was designed to evaluate the effect of season on in vivo oocyte recovery and embryo production in Mediterranean Italian buffalo (Bubalus bubalis). For this purpose repeated transvaginal ultrasound-guided ovum pick up (OPU) was conducted twice a week throughout autumn, mid-winter (transitional period) and spring-summer. The number and size of follicles was determined before puncture. The recovered oocytes were first classified in morphological categories and then used for in vitro embr ... | 2012 | 21872310 |
| Diversity analysis of methanogens in rumen of Bubalus bubalis by 16S riboprinting and sequence analysis. | The molecular diversity of rumen methanogens was investigated by 16S rDNA gene library prepared from the rumen contents obtained from Murrah buffaloes in India. Genomic DNA was isolated from adult male fistulated buffaloes and PCR conditions were set up using specific primers. Amplified product was cloned into a suitable vector, and the positive clones were selected assuming based on blue-white screening and sequenced. Positive clones were reamplified and the resulting PCR products were further ... | 2012 | 22155312 |
| molecular mining of ggaa tagged transcripts and their expression in water buffalo bubalus bubalis. | repeat sequences are involved in regulation of gene expression both at the transcriptional and translational level. in the mammalian genomes, tri- and tetranucleotide repeats like ata, aata, ggaa and gaaa have been associated with diseases. in silico analysis of (ggaa)(5) distribution across the species showed maximum number of this repeat in the mouse transcriptome compared to that in other species. following this, we conducted minisatellite associated sequence amplification (masa) to explore t ... | 2012 | 22037482 |
| advanced comparative cytogenetic analysis of x chromosomes in river buffalo, cattle, sheep, and human. | based on a recently generated comprehensive gene map for ovis aries chromosome x (oarx) with an approximately even locus distribution, we assigned selected bacterial artificial chromosome (bac) probes corresponding to these oarx loci to bubalus bubalis (bbu) and bos taurus (bta) by comparative fluorescence in-situ hybridization (fish) to improve cytogenetically the x chromosome maps in these species. twenty-five added loci in bbux and btax, respectively, contribute to a more detailed description ... | 2012 | 22669522 |
| population structure and genetic variability in the murrah dairy breed of water buffalo in brazil accessed via pedigree analysis. | the objective of this study was to use pedigree analysis to evaluate the population structure and genetic variability in the murrah dairy breed of water buffalo (bubalus bubalis) in brazil. pedigree analysis was performed on 5,061 animals born between 1972 and 2002. the effective number of founders (fe) was 60, representing 6.32 % of the potential number of founders. the effective number of ancestors (fa) was 36 and the genetic contribution of the 17 most influent ancestors explained 50 % of the ... | 2012 | 22535150 |
| influence of intergeneric/interspecies mitochondrial injection; parthenogenetic development of bovine oocytes after injection of mitochondria derived from somatic cells. | interspecies/intergeneric mitochondrial heteroplasmy can occur in interspecies/intergeneric hybrid embryos or following nuclear transfer. in the present study, intergeneric buffalo (bubalus bubalis) mitochondria (wb-mt) or interspecies murine (mus spretus) mitochondria (m-mt) were injected into bovine (bos taurus) oocytes, and the subsequent embryonic development was characterized. fibroblast mitochondria (wb-mt or m-mt) were microinjected into in vitro matured bovine oocytes followed by oocyte ... | 2012 | 22447326 |
| chromosome fragility in river buffalo cows exposed to dioxins. | fifty river buffalo (bubalus bubalis, 2n = 50) cows reared in two different provinces of campania (southern italy) underwent cytogenetic investigations to ascertain possible differences in their chromosome stability. one group (caserta province) was under legal sequestration due to the presence in the milk mass of higher mean values of dioxins [21.79 pg/g of fat as sum of polychloro-dibenzo-dioxins (pcdds), polychloro-dibenzo-furans (pcdfs) and dioxin-like polychlorobiphenyls (dl-pcbs)] than bot ... | 2012 | 22415351 |
| animal production systems of small farms in the kaski district of nepal. | the aim of this study was to describe animal production on small farms in the kaski district of nepal, with the goal of identifying areas where animal health and productivity could be improved. eighty-five randomly selected farms from four different village development committees were visited. farmers were interviewed and premises and animals visually inspected on all farms. feed samples were collected from a subset of farms. the most commonly kept species were water buffalo (used for milk and m ... | 2012 | 22392548 |
| short communication: prevalence and risk factors of subclinical mastitis as determined by the california mastitis test in water buffaloes (bubalis bubalis) in nueva ecija, philippines. | a retrospective analysis using records of lactating bulgarian murrah buffaloes subjected to the california mastitis test in a herd in nueva ecija, philippines was done to determine the prevalence of subclinical mastitis (scm) and to identify risk factors that may influence its occurrence and recurrence. results showed that scm prevalence was 42.76%, whereas its recurrence was 75.03%. age and lactation length influenced the occurrence of scm. in contrast to the conclusions for dairy cows, younger ... | 2012 | 22365218 |
| an association analysis between oxt genotype and milk yield and flow in italian mediterranean river buffalo. | the aim of this study was to evaluate possible associations between three snps at the oxytocin locus (am234538: g.28c>t; g.204a>g and g.1627g>t) and two productive traits, milk yield and milkability, in italian mediterranean river buffaloes. effects of parity, calving season and month of production were also evaluated. a total of 41 980 test-day records belonging to 219 lactations of 163 buffalo cows were investigated. the allele call rate was 98·8% and the major allele frequency for all the inv ... | 2012 | 22280971 |
| male-specific repeats in wild bovidae. | in this study, we investigated repetitive sequences localized on y chromosomes. repetitive dna sequences represent a substantial part of the eukaryotic genome and, among them, a large portion comprises sequences repeated in tandem. efficient and rapid isolation of repeat units is possible due to a laser microdissection technique used for y chromosome separation, followed by polymerase chain reaction (pcr), cloning, and sequence analysis. we applied the derived repeat units to members of nine tri ... | 2012 | 22895838 |
| interferon stimulated gene 15 (isg15): molecular characterization and expression profile in endometrium of buffalo (bubalus bubalis). | interferon stimulated gene 15 (isg15), one of the several proteins induced by conceptus derived type i and/or a type ii interferon (ifn), is implicated as an important factor in determining the uterine receptivity and conceptus development. however, presence as well as specific role of the isg15 in buffalo (bubalus bubalis) reproduction is yet to be elucidated. in the present study, both genomic and cdna sequences of bubaline (bu) isg15 were cloned and investigated for its expression in differen ... | 2012 | 22871329 |
| metagenomic analysis of virulence-associated and antibiotic resistance genes of microbes in rumen of indian buffalo (bubalus bubalis). | a major research goal in rumen microbial ecology is to understand the relationship between community composition and its function, particularly involved in fermentation process is of a potential interest. the buffalo rumen microbiota impacts human food safety as well as animal health. although the bacteria of bovine rumen have been well characterized, techniques have been lacking to correlate total community structure with gene function. we applied 454 next generations sequencing technology to c ... | 2012 | 22850272 |
| new method for the simultaneous identification of cow, sheep, goat, and water buffalo in dairy products by analysis of short species-specific mitochondrial dna targets. | a novel method is presented here as an analytical tool for food control and authentication of dairy products manufactured from the milk of cow, sheep, goat, and buffalo. the method is based on multiplex polymerase chain reaction (pcr) of species-specific mitochondrial dna (mtdna) targets followed by fragment size analysis by capillary electrophoresis. the method includes (a) simultaneous detection of four species, (b) internal control for dna extraction and pcr, (c) mtdna as a target for pcr, (d ... | 2012 | 23025240 |
| a single nucleotide polymorphism in the promoter region of river buffalo stearoyl coa desaturase gene (scd) is associated with milk yield. | an association study between the milk yield trait and the stearoyl-coa desaturase (scd) polymorphism (g.133a > c) in italian mediterranean river buffalo was carried out. a full characterization of the river buffalo scd promoter region was presented. genotyping information was provided and a quick method for allelic discrimination was developed. the frequency of the c allele was 0·16. test-day (td) records (43 510) of milk production belonging to 226 lactations of 169 buffalo cows were analysed w ... | 2012 | 22994977 |
| a novel procedure for precise quantification of schistosoma japonicum eggs in bovine feces. | schistosomiasis japonica is a zoonosis with a number of mammalian species acting as reservoir hosts, including water buffaloes which can contribute up to 75% to human transmission in the people's republic of china. determining prevalence and intensity of schistosoma japonicum in mammalian hosts is important for calculating transmission rates and determining environmental contamination. a new procedure, the formalin-ethyl acetate sedimentation-digestion (fea-sd) technique, for increased visualiza ... | 2012 | 23166847 |
| ultrastructural observation and gene expression profiling of schistosoma japonicum derived from two natural reservoir hosts, water buffalo and yellow cattle. | water buffalo and yellow cattle are the two of the most important natural reservoir hosts for schistosoma japonicum in endemic areas of china, although their susceptibility differs, with water buffalo being less conducive to the growth and development of s. japonicum. results from the current study show that the general morphology and ultrastructure of adult schistosomes derived from the two hosts also differed. using high-throughput microarray technology, we also compared the gene expression pr ... | 2012 | 23110087 |
| [screening and verification on characteristic differentially expressed genes of schistosoma japonicum from three reservoir hosts]. | to get the characteristic differentially expressed genes of schistosoma japonicum from three important reservoir hosts: yellow cattle, water buffalo and goat, so as to find the genetic markers to identify the various sources of the parasite reservoir hosts. | 2012 | 23012949 |
| serum acute phase proteins in control and theileria annulata infected water buffaloes (bubalus bubalis). | this study was carried out to ascertain the changes in acute phase proteins (apps) and pro-inflammatory cytokines in theileria annulata infected water buffalo. thirty infected water buffaloes and 20 parasitologically free were used. in the present study there was significant (p ≤ 0.05) increase in haptoglobin (hp), serum amyloid a (saa), ceruloplasmin, α1-acid glycoprotein (agp) and fibrinogen levels (2.18 ± 0.29 g/l, 156.58 ± 3.48 mg/l, 31.23 ± 1.25mg/dl, 370.23 ± 33.21 mg/l and 16.17 ± 1.18 g/ ... | 2012 | 22785130 |
| can rare positive interactions become common when large carnivores consume livestock? | livestock populations in protected areas are viewed negatively because of their interaction with native ungulates through direct competition for food resources. however, livestock and native prey can also interact indirectly through their shared predator. indirect interactions between two prey species occur when one prey modifies either the functional or numerical responses of a shared predator. this interaction is often manifested as negative effects (apparent competition) on one or both prey s ... | 2012 | 22624309 |
| polymorphism and haplotype structure in river buffalo (bubalus bubalis) toll-like receptor 5 (tlr5) coding sequence. | most of the 160 million river buffalo in the world are in asia where they are used extensively, both as a food source and for draught power. only recently have investigations begun exploring the buffalo genome for variation that might influence health and productivity of these economically important animals. this paper describes the sequence variability of the toll-like receptor 5 (tlr5) gene, which recognizes bacterial flagellin and is a key player in the immune system. tlr5 is comprised of a s ... | 2012 | 22537062 |