Publications

hypocretin-2-saporin lesions of the lateral hypothalamus produce narcoleptic-like sleep behavior in the rat.	hypocretins (hcrts) are recently discovered peptides linked to the human sleep disorder narcolepsy. humans with narcolepsy have decreased numbers of hcrt neurons and hcrt-null mice also have narcoleptic symptoms. hcrt neurons are located only in the lateral hypothalamus (lh) but neither electrolytic nor pharmacological lesions of this or any other brain region have produced narcoleptic-like sleep, suggesting that specific neurons need to be destroyed. hcrt neurons express the hcrt receptor, and ...	2001	11549737
design, synthesis, and biological evaluation of novel nucleoside and nucleotide analogues as agents against dna viruses and/or retroviruses.	a novel strategy was developed for the synthesis of n(7)-purine acyclic nucleosides 9 and 14. the key step involved the reaction between [2-(p-methoxyphenyloxy)ethoxy]methyl chloride and n(9)-tritylated nucleobases 6 or 11 followed by concomitant self-detritylation. n(7)-guanine acyclic nucleoside 9 exhibited antiviral activity, but was phosphorylated by both hsv and vero cell thymidine kinases. thus, it showed more potent cellular toxicity than acyclovir (2). n(7)-adenine acyclic nucleoside 14 ...	2001	11606136
[influence of newly synthesized sesquiterpenes--analogs of taxol on multiplication of herpes simplex virus (hsv-1mc) and retrovirus (mo-msv)].	the study comprised newly synthesized sesquiterpenoid analogs of taxol. the synthesis of the compounds was performed at the institute of organic chemistry, polish academy of sciences. cytotoxicity of the compound was assessed using formazan method. in in vitro studies the cell cultures were infected with hsv-1mc. the tested compounds were added in different concentrations to the cell culture after viral infection. titer of the virus was expressed in tcid50/ml at particular stages of the experime ...	2001	11785186
antiretrovirus activity of a novel class of acyclic pyrimidine nucleoside phosphonates.	a novel class of acyclic nucleoside phosphonates has been discovered in which the base consists of a pyrimidine preferably containing an amino group at c-2 and c-4 and a 2-(phosphonomethoxy)ethoxy (pmeo) or a 2-(phosphonomethoxy)propoxy (pmpo) group at c-6. the 6-pmeo 2,4-diaminopyrimidine (compound 1) and 6-pmpo 2,4-diaminopyrimidine (compound 11) derivatives showed potent activity against human immunodeficiency virus (hiv) in the laboratory (i.e., cem and mt-4 cells) and in primary (i.e., peri ...	2002	12069973
thyroid cell transformation requires the expression of the hmga1 proteins.	elevated expression of hmga1 and hmga2 proteins is correlated with a highly malignant phenotype in several human tumors. we previously demonstrated that the block of hmga2 protein synthesis prevented rat thyroid cell transformation by murine retroviruses. suppression of hmga2 synthesis was associated with lack of induction of hmga1 proteins suggesting that both hmga1 and hmga2 play a role in the process of neoplastic transformation. to determine the role of the hmga1 gene in thyroid cell transfo ...	2002	12082527
anti-viral activity of red microalgal polysaccharides against retroviruses.	red microalgal polysaccharides significantly inhibited the production of retroviruses (murine leukemia virus- mulv) and cell transformation by murine sarcoma virus(musv-124) in cell culture. the most effective inhibitory effect of these polysaccharides against both cell transformation and virus production was obtained when the polysaccharide was added 2 h before or at the time of infection. although, addition of the polysaccharide post-infection significantly reduced the number of transformed ce ...	2002	12204093
spectroscopic characterization of human and mouse primary cells, cell lines and malignant cells.	fourier transform infrared (ftir) spectroscopy is currently being developed as a new optical approach to the diagnosis and characterization of cell or tissue pathology. the advantage of ftir microspectroscopy over conventional ftir spectroscopy in the diagnosis of malignancies is that it facilitates inspection of restricted regions of the cell culture or tissue. in this study, we set out to evaluate ftir microspectroscopy as a diagnostic tool for identifying retrovirus-induced malignancies. our ...	2002	12405154
functional analysis of the murine sarcoma virus rna packaging sequence.	we investigated the features of the moloney murine sarcoma virus leader sequence necessary for rna packaging function by using a deletion analysis approach. we found that sequences that extend beyond those characterized genetically in previous reports are important for optimal packaging efficiency. a fragment covering a minimum of four potential stem-loop structures is required for the shortest packaging element compatible with gene transfer. our results reveal the extent to which each of the se ...	2002	11932430
quantitative imaging of the t cell antitumor response by positron-emission tomography.	we describe a noninvasive, quantitative, and tomographic method to visualize lymphocytes within the whole animal. we used positron-emission tomography (pet) to follow the localization of adoptively transferred immune t lymphocytes. splenic t cells from animals that had rejected a moloney murine sarcoma virus/moloney murine leukemia virus (m-msv/m-mulv)-induced tumor were marked with a pet reporter gene, injected into tumor-bearing mice, and imaged in a micropet by using a substrate specific for ...	2003	12547911
ftir microspectroscopy of malignant fibroblasts transformed by mouse sarcoma virus.	fourier transform infrared microspectroscopy (ftir-msp), which is based on the characteristic molecular vibrational spectra of cells, was used to investigate spectral differences between normal primary rabbit bone marrow (bm) cells and bone marrow cells transformed (bmt) by murine sarcoma virus (musv). primary cells, rather than cell lines, were used for this research because primary cells are similar to normal tissue cells in most of their characteristics. our results showed dramatic changes in ...	2003	12628697
antiretroviral activity of semisynthetic derivatives of glycopeptide antibiotics.	a variety of semisynthetic derivatives of natural antibacterial glycopeptide antibiotics such as vancomycin, eremomycin, ristocetin a, teicoplanin a(2)-2, da-40926, their aglycons, and also the products of their partial degradation with a destroyed or modified peptide core show marked anti-retroviral activity in cell culture. in particular, aglycon antibiotic derivatives containing various substituents of a preferably hydrophobic nature displayed activity against human immunodeficiency virus typ ...	2003	12801238
synthesis and antiviral activity of some 5'-n-phthaloyl-3'-azido-2',3'-dideoxythymidine analogues.	a variety of substituted 5'-n-phthaloyl-3'-azido-2',3'-dideoxythymidine derivatives has been evaluated for their activity against hiv-1, hiv-2 and moloney murine sarcoma virus (msv) in cell culture. most of the 3'-azido-2',3'-dideoxythymidine (azt, zidovudine) derivatives showed antiviral activity in the lower micromolar concentration range and there was a close correlation between their anti-hiv and anti-msv activity (r = 0.99). the adamantyl phthaloyl derivative was active at submicromolar con ...	2003	14521330
5-substituted-2,4-diamino-6-[2-(phosphonomethoxy)ethoxy]pyrimidines-acyclic nucleoside phosphonate analogues with antiviral activity.	2,4-diamino-6-hydroxypyrimidines substituted in position 5 by an allyl, benzyl, cyanomethyl, ethoxycarbonylmethyl, phenyl, cyclopropyl, or methyl group were prepared either by c5-alkylation or by formation of the pyrimidine ring by cyclization. their alkylation with 2-[(diisopropoxyphosphoryl)methoxy]ethyl tosylate afforded n1- and o6-regioisomers that were separated and converted to the free phosphonic acids by treatment with bromotrimethylsilane followed by hydrolysis. reaction of 2,4-diamino- ...	2003	14584956
studies on a transplantable murine rhabdomyosarcoma.	well-differentiated rhabdomyosarcomas developed in 8 of 10 balb/c mice inoculated with cell-free extracts of two lesions arising in bats previously inoculated with the moloney strain of murine sarcoma virus. the tumors could be transplanted in balb/c mice and other strains of mice compatible at the h-2 locus. a tumorigenic clonal cell culture line (r2) was established from a transplanted tumor. ultrastructural studies of the primary and transplanted tumors and r2 cells revealed thick and thin my ...	2003	165312
construction of retroviral vectors with enhanced efficiency of transgene expression.	retroviral vectors have been widely used in gene therapy due to their simple genomic structure and high transduction efficiency. we report a construction of moloney murine sarcoma virus (momsv) and moloney murine leukemia virus (momlv) hybrid-based retroviral vectors with significantly improved efficiency of transgene expression after stable incorporation into the host genome. in these vectors, the residual gag gene coding sequence located in the extended region of packaging signal was removed. ...	2004	15381349
regulation of apoptosis by fau revealed by functional expression cloning and antisense expression.	functional expression cloning is a powerful strategy for identifying critical steps in biological pathways independently of prior assumptions. it is particularly suitable for the identification of molecules crucial to the control of apoptosis. our screen for sequences suppressing t-cell apoptosis isolated a sequence antisense to fau (finkel-biskis-reilly murine sarcoma virus (fbr-musv)-associated ubiquitously expressed gene). the fox gene in fbr murine osteosarcoma virus is also antisense to fau ...	2004	15543234
6-[2-phosphonomethoxy)alkoxy]-2,4-diaminopyrimidines: a new class of acyclic pyrimidine nucleoside phosphonates with antiviral activity.	acyclic nucleoside phosphonate derivatives containing a pyrimidine base preferably bearing amino groups at c-2 and c-4 (dapym), and linked at the c-6 position to (s)-[3-hydroxy-2-(phosphonomethoxy)propoxy] (hpmpo), 2-(phosphonomethoxy) ethoxy (pmeo) or (r)-[2-(phosphonomethoxy)propoxy] (pmpo), display an antiviral sensitivity spectrum that closely mimic that of the parental (s)-hpmp-, pme- and (r)-pmp-purine derivatives. several pmeo-dapym derivatives proved as potent as pmea (adefovir) and (r)- ...	2004	15571252
single point mutations in the zinc finger motifs of the human immunodeficiency virus type 1 nucleocapsid alter rna binding specificities of the gag protein and enhance packaging and infectivity.	a specific interaction between the nucleocapsid (nc) domain of the gag polyprotein and the rna encapsidation signal (psi) is required for preferential incorporation of the retroviral genomic rna into the assembled virion. using the yeast three-hybrid system, we developed a genetic screen to detect human immunodeficiency virus type 1 (hiv-1) gag mutants with altered rna binding specificities. specifically, we randomly mutated full-length hiv-1 gag or its nc portion and screened the mutants for an ...	2005	15919928
cytotoxic, antiviral (in-vitro and in-vivo), immunomodulatory activity and influence on mitotic divisions of three taxol derivatives: 10-deacetyl-baccatin iii, methyl (n-benzoyl-(2'r,3's)-3'-phenylisoserinate) and n-benzoyl-(2'r,3's)-3'-phenylisoserine.	the aim of this study was to evaluate cytotoxic, antiviral (in-vitro and in-vivo) and immunomodulatory activity, as well as the influence on mitotic division, of three taxol derivatives representing modified parts of its molecule: 10-deacetyl-baccatin iii, methyl (n-benzoyl-(2'r,3's)-3'-phenylisoserinate) and n-benzoyl(2'r,3's)-3'-phenylisoserine. the cytotoxicity of the compounds, assessed by the formazane method, was relatively low, with a 50% cytotoxic concentration (cc50)>500 microg ml-1. mo ...	2005	15969936
dimer linkage structure in retroviruses: models that include both duplex and quadruplex domains.	genome of all known retroviruses consists of two identical molecules of rna, which are non-covalently linked. the most stable contact site between two rna molecules is located near their 5' ends. the molecular interactions in the dimer linkage structure (dls) in mature virions are currently unknown. recently we suggested that the dimer linkage structure in human immunodeficiency virus 1 (hiv-1) contains both duplex and quadruplex domains and proposed a model of dls in hiv-1mal (central african v ...	2005	16335231
rna flexibility in the dimerization domain of a gamma retrovirus.	retroviruses are the causative agents of serious diseases, such as acquired immunodeficiency syndromes and several cancers, and are also useful gene therapy vectors. retroviruses contain two sense-strand rna genomes, which become linked at their 5' ends to form an rna dimer. understanding the molecular basis for dimerization may yield new approaches for controlling viral infectivity. because this rna domain is highly conserved within retrovirus groups, it has not been possible to define a consen ...	2005	16408007
fluvastatin does not elevate periosteal osteogenesis induce by moloney sarcoma virus (msv) in mice.	several studies have demonstrated the pleiotropic effects of statins. since wang and associates reported that in rabbits lovastatin reduced steroid-induced bone loss, numerous authors have confirmed these data, however, others have reported conflicting results. in this study, the effects of fluvastatin on bone formation were investigated in early and late phase of osteogenesis. in the first set of experiments (early phase of osteogenesis) cfw/ll mice were randomly divided into three groups. two ...	2006	16531631
high frequency of brafv600e mutation in acquired nevi and small congenital nevi, but low frequency of mutation in medium-sized congenital nevi.	to investigate whether the frequency of the braf(v600e) (v-raf murine sarcoma virus oncogene homolog b1) mutation in melanocytic nevi is associated with sun exposure patterns, we examined 120 acquired melanocytic nevi excised from various anatomic sites, including glabrous skin, as well as 62 congenital nevi. we used a new mutation detection system based on the shifted termination assay, called mutector, which was able to detect only 5% of heterozygous mutant cells within the samples. we detecte ...	2006	16691193
functional replacement of the r region of simian immunodeficiency virus-based vectors by heterologous elements.	substitution of lentiviral cis-acting elements by heterologous sequences might allow the safety of lentiviral vectors to be enhanced by reducing the risk of homologous recombination and vector mobilization. therefore, a substitution and deletion analysis of the r region of simian immunodeficiency virus (siv)-based vectors was performed and the effect of the modifications on packaging and transfer by siv and human immunodeficiency virus type 1 (hiv-1) particles was analysed. deletion of the first ...	2006	16847126
architecture of a gamma retroviral genomic rna dimer.	retroviral genomes contain two sense-strand rnas that are noncovalently linked at their 5' ends, forming a dimer. establishing a structure for this dimer is an obligatory first step toward understanding the fundamental role of the dimeric rna in retroviral biology. we developed a secondary structure model for the minimal dimerization active sequence (midas) for the moloney murine sarcoma virus in the final dimer state using selective 2'-hydroxyl acylation analyzed by primer extension (shape). in ...	2006	17042483
mature murine b lymphocytes immortalized by kirsten sarcoma virus.	clonal, antigen-specific, functionally responsive cell populations have proved critical for the analysis of the activation and regulation of lymphocytes. such studies with b lymphocytes, the precursors of antibody-secreting cells, are hampered by the difficulty in generating phenotypically mature, antigen-reactive lines from defined cell populations. one method is to use acutely transforming retroviruses, which can transform b-lineage lymphocytes in vitro. however, abelson murine leukaemia virus ...	2007	3024015
enhanced viral transformation of skin fibroblasts from neurofibromatosis patients.	enhanced viral transformation of cultured skin fibroblasts (sf) from patients with neurofibromatosis (nf) was observed, compared with cultures established from normal, age-matched controls. cultures of skin fibroblasts from persons with and without clinical nf in families in which the disorder had been diagnosed were examined for transformability by kirsten murine sarcoma virus. the viral transformation results were compared with those obtained with sf cultures initiated from controls in familie ...	2007	6404212
pronounced in vitro and in vivo antiretroviral activity of 5-substituted 2,4-diamino-6-[2-(phosphonomethoxy)ethoxy] pyrimidines.	to discover new potent and selective anti-human immunodeficiency virus (hiv) acyclic nucleoside phosphonate (anp) drugs with in vivo antiretroviral activity.	2007	17124193
transcriptional profile of ki-ras-induced transformation of thyroid cells.	in the last years, an increasing number of experiments has provided compelling evidence for a casual role of ras protein mutations, resulting in their constitutive activation, in thyroid carcinogenesis. however, despite the clear involvement of ras proteins in thyroid carcinogenesis, the nature of most of the target genes, whose expression is modulated by the ras-induced signaling pathways and that are ultimately responsible for ras-induced cellular transformation, remains largely unknown. to an ...	2007	17612936
monitoring of viral cancer progression using ftir microscopy: a comparative study of intact cells and tissues.	fourier transform infrared microspectroscopy (ftir-msp) is an analytical method with a promising potential for detecting the spectral changes due to cancerous changes in cells. the purpose of the present study is monitoring biochemical spectral changes accompanying viral cancer progression in cells and tissues using ftir-msp. as a model system, we used cells in culture which were transformed to malignant cells by infection with murine sarcoma virus (musv) and cervical tissues at different neopla ...	2008	18588944
dysregulated expression of fau and melk is associated with poor prognosis in breast cancer.	programmed cell death through apoptosis plays an essential role in the hormone-regulated physiological turnover of mammary tissue. failure of this active gene-dependent process is central both to the development of breast cancer and to the appearance of the therapy-resistant cancer cells that produce clinical relapse. functional expression cloning in two independent laboratories has identified finkel-biskis-reilly murine sarcoma virus-associated ubiquitously expressed gene (fau) as a novel apopt ...	2009	19671159
rack-1 overexpression protects against goniothalamin-induced cell death.	goniothalamin, a styryllactone, has been shown to induce cytotoxicity via apoptosis in several tumor cell lines. in this study, we have examined the potential role of several genes, which were stably transfected into t-cell lines and which regulate apoptosis in different ways, on goniothalamin-induced cell death. overexpression of full-length receptor for activated protein c-kinase 1 (rack-1) and pc3n3, which up-regulates endogenous rack-1, in both jurkat and w7.2 t cells resulted in inhibition ...	2009	19698770
fau regulates carboplatin resistance in ovarian cancer.	the development of chemotherapy resistance by cancer cells is complex, using different mechanisms and pathways. the gene fau (finkel-biskis-reilly murine sarcoma virus (fbr-musv)-associated ubiquitously expressed gene) was identified through functional expression cloning and previous data have shown that overexpression enhances apoptosis in several cell types. we demonstrate that the expression of fau was reduced in the a2780cis (cisplatin resistant subclone of a2780) cell line compared with the ...	2010	19830698
primary neoplasms of bones in mice: retrospective study and review of literature.	to compare and summarize the mechanisms, frequencies of occurrence, and classification schemes of spontaneous, experimental, and genetically engineered mouse skeletal neoplasms, the literature was reviewed, and archived case material at the jackson laboratory was examined. the frequency of occurrence of spontaneous bone neoplasms was less than 1% for most strains, with the exceptions of osteomas in cf-1 (5.5% and 10% in two studies) and of-1 outbred strains (35%), and osteosarcomas in nod/shiltj ...	2011	21343597
synthesis and antiviral activity of n9-[3-fluoro-2-(phosphonomethoxy)propyl] analogues derived from n6-substituted adenines and 2,6-diaminopurines.	an efficient method for the synthesis of n(9)-[3-fluoro-2-(phosphonomethoxy)propyl] (fpmp) derivatives of purine bases has been developed. both (r)- and (s)-enantiomers of the n(6)-substituted fpmp derivatives of adenine and 2,6-diaminopurine were prepared and their anti-human immunodeficiency virus (hiv) and anti-moloney murine sarcoma virus (msv) activity was evaluated. whereas none of the 6-substituted fpmpa derivatives showed any antiviral activity, several fpmpdap derivatives had a moderate ...	2011	21429755
lack of braf(v600e) mutations in giant congenital melanocytic nevi in a chinese population.	giant congenital melanocytic nevi (cmns) are at an increased risk for malignant transformation. to explore the mutation frequencies of braf(v600e) (v-raf murine sarcoma virus oncogene homolog b1) and nras (neuroblastoma ras viral oncogene homolog) codon 61 in cmns of chinese, we selected 55 paraffin-embedded tissue blocks, including 37 cases of medium cmns (1.5-20cm) and 18 cases of giant cmns (>20 cm). direct sequencing was performed to detect the braf(v600e) and nras codon 61 mutations. the br ...	2011	21430505
n-linked glycosylation regulates human proteinase-activated receptor-1 cell surface expression and disarming via neutrophil proteinases and thermolysin.	proteinase-activated receptor 1 (par(1)) induces activation of platelet and vascular cells after proteolytic cleavage of its extracellular amino terminus by thrombin. in pathological situations, other proteinases may be generated in the circulation and might modify the responses of par(1) by cleaving extracellular domains. in this study, epitope-tagged wild-type hpar(1) and a panel of n-linked glycosylation-deficient mutant receptors were permanently expressed in epithelial cells (knrk and cho c ...	2011	21550978
mos in the oocyte: how to use mapk independently of growth factors and transcription to control meiotic divisions.	in many cell types, the mitogen-activated protein kinase (mapk) also named extracellular signal-regulated kinase (erk) is activated in response to a variety of extracellular growth factor-receptor interactions and leads to the transcriptional activation of immediate early genes, hereby influencing a number of tissue-specific biological activities, as cell proliferation, survival and differentiation. in one specific cell type however, the female germ cell, mapk does not follow this canonical sche ...	2011	21637374
protection of hydroquinone-induced apoptosis by downregulation of fau is mediated by nqo1.	the fau gene (finkel-biskis-reilly murine sarcoma virus (fbr-musv)-associated ubiquitously expressed gene) was identified as a potential tumor suppressor gene using a forward genetics approach. downregulation of fau by overexpression of its reverse sequence has been shown to inhibit apoptosis induced by dna-damaging agents. to address a potential role of fau in benzene toxicity, we investigated the apoptotic effects of hydroquinone (hq), a major benzene metabolite, in w7.2 mouse thymoma cells tr ...	2012	22687461
uptake of cadmium-109, a metallothionein-binding radiometal, by tumors in mice as a function of the transformed phenotype.	metallothionein (mt) is an intracellular protein that binds many metals with isotopes having imaging or radiotherapeutic potential. to determine whether uptake of radioisotopes that bind to mt is increased in tumors, we measured the uptake of cadmium-109 (cd-109) in tumors and in normal tissues of mice. tumors were grown in balb/c mice from cultured balb/3t3 cells transformed by the moloney murine sarcoma virus (mmsv). uptake of cd-109 by mmsv tumors exceeded that by normal tissues examined, wit ...	2012	3372178
expression of c-mos proto-oncogene in undifferentiated teratocarcinoma cells.	proto-oncogene c-mos, the cellular homologue of the transforming gene of moloney murine sarcoma virus, has been characterized by the lack of expression in a variety of differentiated tissues, possibly because of the existence of an inhibitory upstream sequence. we detected mos-related transcripts in undifferentiated embryonal carcinoma cells of pluripotential cell line 311. the sizes of three major transcripts detected were estimated to be 1.8, 4.6 and 6.1-kilobases (kb) by northern analysis. fu ...	2013	3778463
detection and identification of cancerous murine fibroblasts, transformed by murine sarcoma virus in culture, using raman spectroscopy and advanced statistical methods.	cancer is one of the leading worldwide causes of death. it may be induced by a variety of factors, including carcinogens, radiation, genetic factors, or dna and rna viruses. the early detection of cancer is critical for its successful therapy, which can result in complete recovery from some types of cancer.	2013	23671933
cell-surface antigens of murine sarcoma-virus-transformed non-producer cells: further evidence for lack of transplantation immunity.		2014	4376511
design, synthesis, and biological evaluation of unconventional aminopyrimidine, aminopurine, and amino-1,3,5-triazine methyloxynucleosides.	herein we describe a class of unconventional nucleosides (methyloxynucleosides) that combine unconventional nucleobases such as substituted aminopyrimidines, aminopurines, or aminotriazines with unusual sugars in their structures. the allitollyl or altritollyl derivatives were pursued as ribonucleoside mimics, whereas the tetrahydrofuran analogues were pursued as their dideoxynucleoside analogues. the compounds showed poor, if any, activity against a broad range of rna and dna viruses, including ...	2015	25420933
significant differences in integration sites of moloney murine leukemia virus/moloney murine sarcoma virus retroviral vector carrying recombinant coagulation factor ix in two human cell lines.	ligation-mediated-pcr was performed followed by the mapping of 177 and 150 integration sites from hepg2 and hek293 transduced with chimera vector carrying recombinant human factor ix (rhfix) cdna, respectively. the sequences were analyzed for chromosome preference, cpg, transcription start site (tss), repetitive elements, fragile sites and target genes. in hepg2, rhfix was had an increased preference for chromosomes 6 and 17; the median distance to the nearest cpg islands was 15,240 base pairs a ...	2015	25650340
functional and structural characterization of fau gene/protein from marine sponge suberites domuncula.	finkel-biskis-reilly murine sarcoma virus (fbr-musv) ubiquitously expressed (fau) gene is down-regulated in human prostate, breast and ovarian cancers. moreover, its dysregulation is associated with poor prognosis in breast cancer. sponges (porifera) are animals without tissues which branched off first from the common ancestor of all metazoans. a large majority of genes implicated in human cancers have their homologues in the sponge genome. our study suggests that fau gene from the sponge suberi ...	2015	26198235
ikaros deficiency in host hematopoietic cells separates gvl from gvhd after experimental allogeneic hematopoietic cell transplantation.	the graft-versus-leukemia (gvl) effect following allogeneic hematopoietic stem cell transplantation (allo-hct) is critical for its curative potential. hwever, gvl is tightly linked to graft-versus-host disease (gvhd). among hematological malignancies, acute lymphoblastic leukemia (all) is the most resistant to gvl, although the reasons for this remain poorly understood. clinical studies have identified alterations in ikaros (ik) transcription factor as the major marker associated with poor outco ...	2015	26140241
early spectral changes of cellular malignant transformation using fourier transform infrared microspectroscopy.	fourier transform infrared microspectroscopy (ftir-msp) is potentially a powerful analytical method for identifying the spectral properties of biological activity in cells. the goal of the present research is the implementation of ftir-msp to study early spectral changes accompanying malignant transformation of cells. as a model system, cells in culture are infected by the murine sarcoma virus (musv), which induces malignant transformation. the spectral measurements are taken at various postinfe ...	2015	17477718
general anesthesia causes epigenetic histone modulation of c-fos and brain-derived neurotrophic factor, target genes important for neuronal development in the immature rat hippocampus.	early postnatal exposure to general anesthesia (ga) may be detrimental to brain development, resulting in long-term cognitive impairments. older literature suggests that in utero exposure of rodents to ga causes cognitive impairments in the first-generation as well as in the second-generation offspring never exposed to ga. thus, the authors hypothesize that transient exposure to ga during critical stages of synaptogenesis causes epigenetic changes in chromatin with deleterious effects on transcr ...	2016	27028464