Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| herpes simplex virus virion host shutoff function. | herpes simplex virus (hsv) virions contain one or more functions which mediate the shutoff of host protein synthesis and the degradation of host mrna. hsv type 1 (hsv-1) mutants deficient in the virion shutoff of host protein synthesis (vhs mutants) were isolated and were found to be defective in their ability to degrade host mrna. furthermore, it was found that viral mrnas in cells infected with the vhs 1 mutant have a significantly longer functional half-life than viral mrnas in wild-type viru ... | 1988 | 2828686 |
| prior herpes simplex virus type 2 infection as a risk factor for hiv infection. | antibodies to cytomegalovirus (cmv), epstein-barr virus (ebv), and herpes simplex viruses types 1 and 2 (hsv-1, hsv-2) in three sequential serum samples from 62 men who did and 61 men who did not develop human immunodeficiency virus (hiv) infection at the time of the final (third) serum specimen were studied. antibody titers to cmv, ebv, and hsv-1 did not significantly rise in or differ between men who did or did not get hiv infection. however, we found that 32 (68%) of 47 hiv seroconverters had ... | 1988 | 2828700 |
| herpes simplex virus glycoprotein treatment of recurrent genital herpes. | we investigated the possibility that herpes simplex virus (hsv) glycoproteins might provide potential immunotherapy for recurrent hsv disease by using the guinea pig model of genital hsv infection. in two experiments, after recovery from initial genital herpes, 58 animals were randomized either to receive a glycoprotein vaccine or to serve as controls. both a mixture of hsv-2 glycoproteins derived from infected tissue cultures and a mixture of hsv-1 glycoproteins b and d, prepared by genetic eng ... | 1988 | 2826603 |
| type-specific antibodies to herpes simplex virus type 2 (hsv-2) glycoprotein g in pregnant women, infants exposed to maternal hsv-2 infection at delivery, and infants with neonatal herpes. | we used a murine monoclonal antibody to herpes simplex virus (hsv) type 2 (hsv-2) glycoprotein g (gg) to develop an enzyme immunoassay that detected hsv-2 type-specific antibodies in human sera. antibodies to hsv-2 gg were detected in 98 (96%) of 102 sera from pregnant women with culture-proved hsv-2 infection. sixty-five percent of the women had serological evidence of past hsv-2 infection by the rawls index, based on titers of neutralizing antibody to hsv type 1 and hsv-2. thirty (88%) of 34 i ... | 1988 | 2826604 |
| generation of a herpes simplex virus type 2 variant devoid of xbai sites: removal of the 0.91 map coordinate site results in impaired synthesis of glycoprotein g-2. | herpes simplex virus (hsv) type 2 (strain hg52) has four xbai sites at map coordinates (m.c.) 0.45, 0.7, 0.91 and 0.94, i.e. two in the unique long and two in the unique short regions of the genome. previously, we had isolated a genome containing only the 0.45 and 0.94 xbai sites. here we report the isolation of a mutant (jh2611) in which all four xbai sites have been removed using an enrichment selection procedure, without any loss of viability. removal of each site has been shown to be due to ... | 1988 | 2826660 |
| herpes simplex virus infection of the hand. a profile of 79 cases. | seventy-nine cases of herpes simplex (hsv) infection of the hand were documented by viral culture over a 40-month period. a marked bimodal age distribution was found with 32 cases occurring in adults aged 21 to 30 years and 16 occurring in children less than 10 years old. the infection most commonly occurred on the fingers (69 percent) and thumb (21 percent). all 20 herpes simplex type 2 (hsv-2) isolates came from patients over 20 years old. all of the 13 specimens that were typed after isolatio ... | 1988 | 2827469 |
| regulation of glycoprotein d synthesis: does alpha 4, the major regulatory protein of herpes simplex virus 1, regulate late genes both positively and negatively? | earlier studies have described the alpha 4/c113 baby hamster kidney cell line which constitutively expresses the alpha 4 protein, the major regulatory protein of herpes simplex virus 1 (hsv-1). introduction of the hsv-1 glycoprotein b (gb) gene, regulated as a gamma 1 gene, into these cells yielded a cell line which constitutively expressed both the alpha 4 and gamma 1 gb genes. the expression of the gb gene was dependent on the presence of functional alpha 4 protein. in this article we report t ... | 1988 | 2824843 |
| entry of herpes simplex virus 1 in bj cells that constitutively express viral glycoprotein d is by endocytosis and results in degradation of the virus. | the bj cell line which constitutively expresses herpes simplex virus 1 glycoprotein d is resistant to infection with herpes simplex viruses. analysis of clonal lines indicated that resistance to superinfecting virus correlates with the expression of glycoprotein d. resistance was not due to a failure of attachment to cells, since the superinfecting virus absorbed to the bj cells. electron microscopic studies showed that the virions are juxtaposed to coated pits and are then taken up into endocyt ... | 1988 | 2824844 |
| a human cytomegalovirus function inhibits replication of herpes simplex virus. | human embryonic lung (hel) cells infected with human cytomegalovirus (hcmv) restricted the replication of herpes simplex virus type 1 (hsv-1). a delay in hsv replication of 15 h as well as a consistent, almost 3 log inhibition of hsv replication in hcmv-infected cell cultures harvested 24 to 72 h after superinfection were observed compared with controls infected with hsv alone. treatment of hcmv-infected hel cells with cycloheximide (100 micrograms/ml) for 3 or 24 h, conditions known to result i ... | 1988 | 2824846 |
| intravenous acyclovir therapy of first episodes of genital herpes: a multicenter double-blind, placebo-controlled trial. | a collaborative multicenter double-blind, placebo-controlled trial of intravenous acyclovir treatment of first-episode genital herpes was performed in order to substantiate previous findings on the efficacy and safety of this drug, to evaluate the influence of parenteral therapy on recurrence frequency, and to obtain further data on the natural history of genital herpes. | 1988 | 3046350 |
| hemorrhagic cystitis due to herpes simplex virus as a marker of disseminated herpes infection. | herpetic cystitis has rarely been reported. herein are described two immunocompromised female patients with genital or buttock herpetic lesions in whom hemorrhagic cystitis due to herpes simplex virus type 2 developed in the setting of disseminated herpetic infection. both patients had indwelling foley catheters. urinary tract instrumentation may cause local or widespread dissemination of genital herpes in immunocompromised patients. | 1988 | 3348273 |
| antiherpesvirus activity of 9-(4-hydroxy-3-hydroxymethylbut-1-yl) guanine (brl 39123) in animals. | the antiviral activity of 9-(4-hydroxy-3-hydroxymethylbut-1-yl)guanine (brl 39123) was assessed in several animal models of herpes simplex virus (hsv) infection. brl 39123 was as active as acyclovir (acv) when applied topically to guinea pigs with a cutaneous hsv type 1 (hsv-1) infection and was also active topically in an hsv-2 genital infection. before systemic administration to infected animals, brl 39123 and acv were administered orally and subcutaneously to mice, and the blood was assayed f ... | 1988 | 3364954 |
| the association between genital ulcer disease and acquisition of hiv infection in homosexual men. | we analyzed the association of herpes simplex virus (hsv) infection and syphilis, the two most common causes of genital ulceration in homosexual men, with human immunodeficiency virus (hiv) infection in 200 men enrolled between 1983 and 1986 into a study evaluating the microbial causes of acute proctitis. infection with hiv was independently associated with a history of syphilis, serologic evidence of syphilis, a history of hsv infection, and antibody to hsv-2. antibody to hiv was not associated ... | 1988 | 3404600 |
| cyclopentenylcytosine. a carbocyclic nucleoside with antitumor and antiviral properties. | cyclopentenylcytosine (cpe-c, 2), a pyrimidine analogue of the fermentation derived carbocyclic nucleoside neplanocin a, has been synthesized from the optically active cyclopentenylamine 3b by two synthetic routes. cpe-c demonstrates significant antitumor activity against both the sensitive and ara-c resistant lines of l1210 leukemia in vivo. multiple long term survivors are produced in both tumor models. the compound also gives 100% growth inhibition of the solid human a549 lung and mx-1 mammar ... | 1988 | 3411597 |
| induction of fc and c3b receptors on rabbit corneal cells by herpes simplex virus. | the expression of receptors for fc portion (fcr) of immunoglobulin g (igg) and for a c3b component of complement (c3br) by herpes simplex virus (hsv) was studied in primary cultures of rabbit corneal cells. monolayer cultures of epithelial, stromal and endothelial cells of the rabbit cornea were infected with three strains each of type 1 (hsv-1) and type 2 hsv (hsv-2). rosette methods were used to detect receptors by means of sheep erythrocytes sensitized with rabbit igg for fcr and c3b-coated s ... | 1988 | 3417420 |
| remyelination of central nervous system lesions in experimental genital herpes simplex virus infection. | to study spinal cord remyelination in a model of genital herpes simplex virus type 2 (hsv-2) infection, adult female mice were inoculated by a vaginal route. at intervals up to 6 months after infection, cord tissues were removed and examined by light and electron microscopy and by immunohistochemical methods. as a consequence of acute infection, 60% of mice developed multifocal central nervous system (cns) demyelinative lesions in the lower thoracic, lumbar, or upper sacral cord. these lesions, ... | 1988 | 3171599 |
| herpes-simplex-related antigen in human demyelinative disease and encephalitis. | using immunohistochemical methods optimized to detect herpes simplex virus type 2 (hsv-2) antigen, paraffin sections from human central nervous system tissues from 31 cases pathologically diagnosed as multiple sclerosis (ms), 34 cases of other neurological diseases, 4 adult cases of hsv encephalitis, and mouse brains infected with various hsv strains were examined. two distinct patterns of immunoreactivity with hsv antisera were seen. in typical acute human and experimental encephalitis, antigen ... | 1988 | 3176900 |
| comparative study of various immunomodulators for macrophage and natural killer cell activation and antiviral efficacy against exotic rna viruses. | several immunomodulators were compared for immunomodulatory and antiviral activity in b6c3f1 female mice. our results demonstrate that murine recombinant gamma interferon (rifn-g), human recombinant alpha a/d interferon (rifn-a), ampligen (a polyribonucleotide) and cl246,738 modulate nonspecific immunity and are effective antiviral agents in vivo. administration of each of these agents 1 day before cell harvest induced high levels of splenic natural killer (nk) cell activity against yac-1 target ... | 1988 | 3182149 |
| expression of herpes simplex virus glycoprotein d on antigen presenting cells infected with vaccinia recombinants and protective immunity. | we studied the effect of the temporal regulation of herpes simplex virus (hsv) type 1 glycoprotein d (gd-1) expression in ia+ epidermal cells (ec) and macrophages on virus specific immunity and protection from hsv-2 challenge. gd-1 was expressed on the surface of cells infected with a vaccinia recombinant containing gd-1 under the control of an early vaccinia virus promoter (vp176). it was not expressed in cells infected with a recombinant (vp254) in which gd-1 is controlled by a late vaccinia v ... | 1988 | 3263886 |
| effects of long-term acyclovir chemosuppression on serum igg antibody to herpes simplex virus. | patients with severe or frequent recurrent genital herpes simplex virus (hsv) infection can be managed either by treating each recurrence with acyclovir or by suppressing recurrences with daily administration of the drug. to determine the effects of long-term acyclovir therapy on the immune response to hsv, we studied the change in igg antibody concentration to hsv in 46 individuals with recurrent genital hsv-2 infection who received acyclovir for 1 year, twenty-seven subjects received daily acy ... | 1988 | 3183633 |
| [ulcerative herpes simplex virus ii esophagitis]. | 1988 | 2831591 | |
| acyclovir-resistant, foscarnet-sensitive oral herpes simplex type 2 lesion in a patient with aids. | we report the case of an immunocompromised patient with aids in whom developed a perioral and several intraoral hsv 2 lesions that persisted for more than 1 year. the virus was resistant to acyclovir but was sensitive to foscarnet. viral isolates were thymidine kinase negative. the lesions resolved with intravenous foscarnet therapy given over a 15-week period, and when last seen, 8 months after foscarnet was discontinued, the patient had not had a recurrence. | 1989 | 2524706 |
| epidemiology & clinical profile of genital herpes. | during 1983-86, genital herpes (gh) was diagnosed in 225 (19%) of 1194 patients attending the std clinic, attached to a medical college in south india. the patients included 169 men and 56 women; 50 had primary gh and 175 had recurrent gh. the prevalence of gh in men and women did not differ significantly but the proportion of primary gh was significantly higher in women. there was no seasonal variation. the clinical epidemiology of these patients is compared with those in other countries. virol ... | 1989 | 2783686 |
| herpes simplex virus type 2 transcripts in trigeminal ganglia during acute and latent infection in mice. | adult mice were inoculated intracerebrally with herpes simplex virus type 2 (hsv-2), and perfused at days 5, 8, 81, 108, and 130 after infection. trigeminal ganglia and roots were dissected out and embedded in paraffin. four 35s-labelled dna probes that contained immediate-early (icp-0, icp-4), delayed-early (thymidine kinase; tk), or late (morphological transforming region; mtr) genes were prepared. in situ hybridization methods or an immunoperoxidase antigen method using hsv-2 antibody were ap ... | 1989 | 2592985 |
| a self-inducing runaway-replication plasmid expression system utilizing the rop protein. | a highly efficient prokaryotic expression system has been developed that produces proteins at levels exceeding 150 micrograms/ml of culture medium. the system consists of a temperature-sensitive-copy-number plasmid that carries the rop gene and promoter downstream from the trp promoter. any sequence cloned into the pvuii site of the rop gene alters rop protein activity and causes lethal runaway plasmid dna replication. this plasmid replication can be suppressed in trans by complementation with a ... | 1989 | 2670683 |
| characterization of a continuous smooth muscle cell line derived from rabbit aorta. | a spontaneously arising continuous cell line (rb-1) derived from collagenase-elastase digested rabbit aorta has been propagated in vitro for over 100 passages. during this period, the rb-1 cells remained spindle-shaped and formed regularly oriented parallel bundles. after passage 50, rb-1 cells were found to be serum-independent in their growth and reached higher saturation density than rabbit aorta smooth muscle cells. alpha-actin and desmin filaments were detected by immunostaining in rb-1 cel ... | 1989 | 2681130 |
| [acyclovir and pregnancy: current aspects]. | acyclovir (acv), an antiviral nucleoside analog, is active against herpes simplex viruses (hsv1, hsv2) and varicella virus (vzv). these viruses seems to be prejudicial to the pregnant woman and to the fetus. yet, acv is not recommended for use in pregnancy. however in certain cases, this drug has been used. we review in this paper, the pharmacokinetics and transplacental passage of acv, indications, and whether the benefits of the administration of acv in pregnancy outweigh the theoretical risks ... | 1989 | 2681370 |
| a novel form of intrauterine infection by herpes simplex virus type 2: its association with spontaneous abortion, latent neonatal infection, and adult inflammatory diseases. | 1989 | 2685911 | |
| [malpighian epithelia infected by dna viruses and langerhans cells]. | malpighian epithelia exhibit an immune surveillance through langerhans cells (lc) against the dna viruses, which induce various epithelial lesions, mainly on the skin, conjunctiva, mouth, uterine cervix. these viral infections are usual during childhood. the most common dna viruses are: adenoviruses (more than 40 types), human papillomaviruses (hpv, more than 50 types) and herpes viruses (herpes simplex type 1 and 2, varicella and zooster, epstein-barr virus). different clinical aspects are obse ... | 1989 | 2694082 |
| [is mollaret's meningitis caused by herpes simplex virus type 2?]. | mollaret's meningitis is characterised by recurrent, brief episodes of serous meningitis with symptom-free intervals. although viral etiology has been suspected, no pathogenic agent has hitherto been found. using the immunoblot technique, we found igg antibodies of intrathecal origin against the hsv-2-specific protein, gg2, in a patient with mollaret's meningitis. since being put on prophylactic acyclovir treatment twelve months ago, the patient has had no further episodes of meningitis. because ... | 1989 | 2709930 |
| 5-(2-bromoethyl)-2'-deoxyuridine: a selective inhibitor of herpes simplex viruses in vitro. | a new pyrimidine analog, 5-(2-bromoethyl)-2'-deoxyuridine (beudr), was tested in vitro for antiviral activity on herpes simplex virus types 1 and 2. as reference compounds, acg, bvudr and paa were used. compared to acg and bvudr, beudr resulted less potent on both hsv-1 and hsv-2. however, a 50% inhibition of the multiplication of uninfected cells could be obtained only at very high beudr concentration (id50 = 8500 microm). this makes beudr the least toxic analog known and gives it a selective i ... | 1989 | 2713989 |
| herpetic stromal keratitis in congenital dysgammaglobulinemia. case report. | the clinical picture and laboratory findings of a case of secondary herpes simplex virus type 2 in a patient with congenital dysgammaglobulinemia who was followed for 26 months is described. local combined therapy of acyclovir and decadron (dexamethasone) 0.1% was given for management for 14 months. the clinical and immunopathologic findings of our patient match the results described by other investigators in experimental animals. | 1989 | 2714093 |
| the inhibitory effect of gossypol on human sperm motility: relationship with time, temperature and concentration. | the inhibitory effect of gossypol acetic acid on human sperm motility was studied with a transmembrane migration method. gossypol decreased sperm motility after it had been incubated with semen for more than 15 min. however, when sperm motility was evaluated immediately after semen had been mixed with gossypol, no inhibitory effect could be found. we consider that the sperm immobilizing potency of gossypol is much less than our previously studied sperm immobilizing agents. it is unlikely that go ... | 1989 | 2714811 |
| efficacy of phosphonylmethoxyalkyl derivatives of adenine in experimental herpes simplex virus and vaccinia virus infections in vivo. | the phosphonylmethoxyalkyl derivatives (s)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine [(s)-hpmpa], 9-(2-phosphonylmethoxyethyl)adenine (pmea), and 9-(2-phosphonylmethoxyethyl)-2,6-diaminopurine (pmedap) were evaluated for their in vivo efficacies in several animal model infections, i.e., mice infected intravenously with vaccinia virus and mice infected intracutaneously, intraperitoneally, or intracerebrally with herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) or thymidine kinase-defici ... | 1989 | 2719463 |
| synthesis and antiviral activity of 5'-o-(substituted) sulfamoyl pyrimidine nucleosides. | 5'-o-[n-(aminoacyl or isobutyryl)sulfamoyl]uridines 4a-e, 5a-e and 5'-o-[n-(isopropyl)sulfamoyl]cytidines 7-9 have been synthesized and tested against herpes simplex virus type 2. condensation of 2',3'-o-isopropylidene-5'-o-sulfamoyluridine with the n-hydroxysuccinimide esters of boc-gly, boc-l-ala, boc-d-ala and boc-l-phe, gave 4a-d which, on deprotection under acidic conditions, provided 5a-d. a similar condensation of 2',3'-di-o-acetyl-5'-o-sulfamoyluridine with the n-hydroxysuccinimide ester ... | 1989 | 2730285 |
| axonal degeneration and regeneration in sensory roots in a genital herpes model. | in a mouse model of genital herpes simplex virus type 2 (hsv-2) infection, roots of the lower spinal cord were examined 5 days to 6 months after inoculation. using immunoperoxidase methods on paraffin sections, viral antigen was found in sensory ganglia, their proximal roots and distal nerves on days 5 and 6 after infection. in epon sections, most mice had focal sensory root abnormalities in lower thoracic, lumbar or sacral levels. at days 7 and 10, lesions showed chiefly nerve fiber degeneratio ... | 1989 | 2750478 |
| two cases of necrotizing myelopathy associated with malignancy caused by herpes simplex virus type 2. | two cases of necrotizing myelopathy were autopsied; one was complicated with lung carcinoma and the other with chronic type adult t cell leukemia (atl leukemia). to our knowledge, they were the first cases of their type in japan. in both cases, necrosis of the spinal cord was observed in the gray and white matter along most of its extent. marked changes were found in the lumbar segment. the patients were not treated with intravenous cancer chemotherapy or irradiation. immunohistochemical and ele ... | 1989 | 2763797 |
| antibody responses and protection in mice immunized with herpes simplex virus type 1 antigen immune-stimulating complex preparations. | the formation of immune-stimulating complexes (iscoms) obtained by mixing the glycoside quil a with an antigen preparation derived from herpes simplex virus type 1 (hsv-1)-infected cell cultures using a zwitterionic detergent is described. the hsv-1 antigen preparation incorporated into iscoms elicited significantly greater antibody responses in mice than the same preparation administered together with aluminium hydroxide gel, and provided complete protection against hsv-1 or hsv-2 lethal, syste ... | 1989 | 2769234 |
| t lymphocytes in genital lymph nodes protect mice from intravaginal infection with herpes simplex virus type 2. | herpes simplex virus type 2 (hsv-2) is a human venereal pathogen that causes lethal neurological illness after intravaginal inoculation into balb/cj mice. intravaginal vaccination of mice with an attenuated strain of hsv-2 rapidly induces immunity to a lethal intravaginal challenge with wild-type hsv-2. this resistance is transferrable to syngeneic mice with genital lymph node (gln) cells but not with cells from other lymphoid sources. here we demonstrate that minimal numbers of hsv-2-stimulated ... | 1989 | 2783720 |
| broad-spectrum in vivo antiviral activity of 7-thia-8-oxoguanosine, a novel immunopotentiating agent. | a novel immunopotentiating agent, 5-amino-3-beta-d-ribofuranosylthiazolo [4,5-d]pyrimidine-2,7(3h,6h)-dione (7-thia-8-oxoguanosine), lacks virus-inhibitory properties in vitro but induces interferon and potentiates immune functions, such as natural killer cell activity. it was evaluated in rodent models to determine the spectrum of antiviral activity and effective treatment regimens. at 50 to 200 mg/kg given as single or divided intraperitoneal (i.p.) doses 1 day before virus inoculation, signif ... | 1989 | 2817849 |
| acyclic analogues of 3'-azido-3'-deoxythymidine as potential antiviral agents. nucleoside synthesis by michael addition. | an acyclic derivative of 3'-azido-3'-deoxythymidine, (r,s)-1-[1-(2-hydroxyethoxy)-3-azidopropyl]thymine (2), has been synthesized by a path involving michael-type addition. related thymidine analogues lacking the c(3')-c(4') bond were similarly obtained. the method provides a versatile new route to nucleoside analogues. the new compounds were found to be essentially inactive against human immunodeficiency virus type 1 (hiv-1) and herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in vitro wh ... | 1989 | 2909745 |
| [viral or bacterial meningitis. differential diagnostic problems]. | a 19 year old previously healthy man presented symptoms of meningitis. culture from the cerebrospinal fluid revealed herpes simplex virus type 2 to be the responsible agent. the course was benign, the patient was never seriously ill. he recovered fully within a few weeks. the cerebrospinal fluid showed prolonged pleocytosis and low values of glucose. the traditional cerebrospinal fluid parameters employed in the diagnosis of meningitis, and for distinguishing bacterial from viral meningitis, are ... | 1989 | 2919375 |
| [herpes simplex virus infection of the central nervous system]. | herpes simplex virus type 1 (hsv 1) infection in the central nervous system results in encephalitis with high mortality and morbidity. however herpes simplex virus type 2 (hsv 2) infection in this area usually results in benign meningitis. brain biopsy is the most sensitive and most specific method for diagnosing hsv 1 encephalitis. antibody determinations in serum and cerebrospinal fluid give only a retrospective diagnosis. a rapid non-invasive diagnostic test is urgently required as an alterna ... | 1989 | 2919376 |
| fine mapping of antigenic site ii of herpes simplex virus glycoprotein d. | glycoprotein d (gd) is a virion envelope component of herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) which plays an important role in viral infection and pathogenesis. previously, anti-gd monoclonal antibodies (mabs) were arranged into groups which recognize distinct type-common and type-specific sites on hsv-1 gd (gd-1) and hsv-2 gd (gd-2). several groups recognize discontinuous epitopes which are dependent on tertiary structure. three groups, vii, ii, and v, recognize continuous epitopes p ... | 1989 | 2467994 |
| targets of infection in a herpes simplex-reactivation model. | in mice surviving intracerebral inoculation with herpes simplex virus type 2 (hsv-2), recurrent infection was induced using high-dose immunosuppressive treatment. targets of reactivated infection were identified in serial sections of heads using immunoperoxidase methods to detect viral antigen. peripheral targets were cranial sensory and autonomic ganglia, peripheral nerves, and many non-neural structures. central neural tissues included retina, optic nerve and tract, lateral geniculate nucleus ... | 1989 | 2469288 |
| topological distribution of virus-specific and cross-reactive antigenic determinants on the gb glycoprotein of the herpes simplex viruses. | the antigenic properties and relations between the herpes simplex virus type 1 and 2 (hsv1, hsv2) gb glycoproteins were investigated. using several assay systems to analyze the virus-specific reactivity of polyclonal monospecific rabbit anti-gb sera, it was demonstrated that most of the antigenic determinants of the gb glycoproteins exposed at the surface of both virions and infected cells are virus-specific rather than cross-reactive. comparative examination of the reactivity of human immune se ... | 1989 | 2470853 |
| a subset of type-specific epitopes map in the amino terminus of herpes simplex virus type 1 glycoprotein b. | we constructed a recombinant herpes simplex virus (hsv) containing the transcribed coding and non-coding sequences of hsv-1 strain f glycoprotein b (gb) gene, a gamma 1 gene, fused to the promoter-regulatory sequences of the hsv-1 alpha 4 gene and inserted into the thymidine kinase gene of rh1g44, an hsv-1 x hsv-2 recombinant that contains an hsv-2 gb gene at the natural locus. phenotypic analyses of the insertion mutant, r3145, showed that the alpha gb gene was transcribed in the presence of cy ... | 1989 | 2471797 |
| properties of the herpes simplex virus type 2 trans-inducing factor vmw65 in wild-type and mutant viruses. | a temperature-sensitive mutant (ts13) of herpes simplex virus type 2 (hsv-2) has a mutation which causes in vitro thermolability of the virion. this mutation lies within the gene encoding a virion structural protein of mr 65k which is known to stimulate immediate early transcription (the trans-inducing factor, 65ktif). the results presented here show that the structural role of 65ktif is essential. the electrophoretic mobility of the 65ktif encoded by ts13 and a revertant of ts13 differed from t ... | 1989 | 2471816 |
| effects of the immunomodulating agent r837 on acute and latent herpes simplex virus type 2 infections. | r837 is an immune modulator with no in vitro activity against herpes simplex virus (hsv). we evaluated topical r837 as therapy for genital hsv type 2 infection using the guinea pig model of this disease. furthermore, we investigated the effect of r837 therapy on acute and latent neural hsv infections. therapy initiated 12 h after viral inoculation and given twice a day significantly reduced the acute neural infection so that hsv was recovered from only 1 of 64 neural tissue specimens obtained fr ... | 1989 | 2479335 |
| effect of 2',5'-oligoadenylate on herpes simplex virus-infected cells and preventive action of 2',5'-oligoadenylate on the lethal effect of hsv-2. | antiviral effects of ppp(a2'p)na, (2-5a) on herpes simplex virus type 1 or type 2 (hsv-1 or hsv-2)-infected baby hamster kidney fibroblasts (bhk cells) and hsv-2-infected female guinea pigs were examined. 2-5a was introduced into bhk cells in the form of a calcium phosphate precipitate and as an ointment of polyethylene glycol (peg) into guinea pig vagina. under optimum conditions, 2-5a trimer and other 2-5a derivatives inhibited over 90% of hsv-1 syncytia formation and over 50% of hsv-2 plaque ... | 1989 | 2481701 |
| relatedness of glycoproteins expressed on the surface of simian herpes-virus virions and infected cells to specific hsv glycoproteins. | the antigenic relatedness of the surface glycoprotein antigens of six herpesviruses indigenous to human and nonhuman primates was examined. binding of anti-viral sera to viral antigens expressed on the surface of infected cells demonstrated that the surface antigens of herpes simplex virus type 1 (hsv 1), hsv 2, simian agent 8 (sa8), and herpesvirus simiae (b virus) exhibit extensive cross-reactivity. surface antigens of two viruses isolated from south american primates, h. saimiri 1 (hvs 1) and ... | 1989 | 2482016 |
| studies of phospholipase and cyclooxygenase activities in herpes simplex virus type 2-transformed rat cells. | in this report we describe results showing that both arachidonic acid (20:4) release and prostaglandin (pg) synthesis in herpes simplex virus type 2 (hsv-2)-transformed tumor-derived rat fibrosarcoma (rfs) cells are inhibited by indomethacin (cyclooxygenase inhibitor). these data suggest that cyclooxygenase (prostaglandin endoperoxide synthetase, pes) and phospholipase are coupled in their regulation in rfs cells. data obtained using a cell-free assay to measure directly cyclooxygenase specific ... | 1989 | 2509090 |
| foscarnet therapy for severe acyclovir-resistant herpes simplex virus type-2 infections in patients with the acquired immunodeficiency syndrome (aids). an uncontrolled trial. | to determine whether trisodium phosphonoformate (foscarnet) is efficacious in treating severe mucocutaneous disease due to acyclovir-resistant herpes simplex virus type-2 (hsv-2) infection in patients with the acquired immunodeficiency syndrome (aids). | 1989 | 2522751 |
| genital infections due to herpes simplex virus type 2 in male guinea pigs. | a small-animal model of genital herpes simplex virus (hsv) infection has not been available for investigating the pathogenesis of disease in males. male hartley guinea pigs were inoculated on the medial thigh with 10(5)-10(6) plaque-forming units of hsv type 2. animals developed vesicular lesions in the genital area, including the penis. transient urinary retention, hindlimb paralysis, and acute urethral shedding of hsv also occurred. during acute infection, virus was detected in neural and geni ... | 1989 | 2535869 |
| effect of human semen on herpes-simplex virus-2. | this study was designed to evaluate the suggestion of inhibition of infectivity of hsv-2 by seminal fluid by examining relationships between hsv-2 and components of human semen. viral infectivity was quantitated by tcid50 measurements of cpe in comparing viral inoculated aliquots of whole semen, seminal fluid, and control (emem) medium during incubation at 0, 5, and 24 hours. statistical analysis of data from 15 experiments revealed inhibition by seminal fluid during 5 hours of incubation. after ... | 1989 | 2535986 |
| viral agents in two-stage cervical carcinogenesis: an experimental study in mice. | the role of chemical and viral agents in the development of cervical cancer in mice was studied by repeated carcinogen applications, repeated intravaginal instillations of hsv type ii virus, and single carcinogen application as initiating agent followed by repeated instillations of virus as promoter. in all the animals studied, repeated applications of 9,10-dimethylbenzanthracene (dmba) induced dysplastic conditions of the cervix and vagina, mild, moderate, and severe, as well as a large number ... | 1989 | 2535998 |
| identification, transfer, and characterization of cloned herpes simplex virus invasiveness regions. | following peripheral inoculation of experimental animals, herpes simplex virus type 2 (hsv-2) strains are more virulent than hsv-1 strains, and clinical studies suggest that they possess enhanced virulence in humans. one dramatic type-specific difference in virulence is observed following inoculation of the chorioallantoic membrane (cam) of the chicken embryo: hsv-2, but not hsv-1, makes large pocks on the cam, invades the mesoderm, generalizes in the embryo, and kills the chicken. these propert ... | 1989 | 2536826 |
| lack of serum antibodies to the major hsv-2 specified dna-binding protein before diagnosis of cervical neoplasia. | we report results based on a prospective case-control study within a cohort obtained by linking the finnish social insurance institutions mobile clinic survey registry and the finnish cancer registry. the overall prevalence of herpes virus early antigen (icsp 11/12) antibodies was similar in controls and in cervical neoplasia cases (consisting of invasive cervical carcinoma, icc, and cervical intraepithelial neoplasia group iii, cin iii cases). however, those cin iii cases, who after a long lag ... | 1989 | 2537883 |
| the antiviral activity of 9-beta-d-arabinofuranosyladenine is enhanced by the 2',3'-dideoxyriboside, the 2',3'-didehydro-2',3'-dideoxyriboside and the 3'-azido-2',3'-dideoxyriboside of 2,6-diaminopurine. | the 2',3'-dideoxyriboside (dddapr), 2',3'-didehydro-2',3'-dideoxyriboside (ddedapr) and 3'-azido-2',3'-dideoxyriboside (azdddapr) of 2,6-diaminopurine have been previously recognized as potent inhibitors of human immunodeficiency virus replication. these compounds are also potent inhibitors of adenosine deaminase and inhibit the deamination of 9-beta-d-arabinofuranosyladenine (araa). dddapr, ddedapr and azdddapr markedly potentiate the antiviral activity of araa against herpes simplex virus type ... | 1989 | 2538128 |
| regulation of expression of herpes simplex virus (hsv) glycoprotein d in vaccinia recombinants affects their ability to protect from cutaneous hsv-2 disease. | the effect of regulation of herpes simplex virus (hsv) type 1 glycoprotein d (gd-1) gene expression on hsv-specific immune response and protection from cutaneous hsv-2 disease was studied using vaccinia virus recombinants containing gd-1 under the control of early (vp176) or late (vp254) vaccinia virus promoters. expression of gd-1 in vp176-infected cells was first observed at 2 h after infection. it did not depend on viral dna replication. in vp254-infected cells, gd-1 was first observed at 24 ... | 1989 | 2538519 |
| transformation of nih 3t3 cells by herpes simplex type 2 bglii n fragment and sub-fragments is independent from induction of mutation at the hprt locus. | the effect of transfection of the herpes simplex virus type 2 transforming fragment bglii n and of its three xhol subfragments on mutagenesis and morphological transformation was assayed in nih 3t3 cells. while bglii n and the right hand portion of this fragment increased the number of transformed foci, no significant effects on the mutation frequency at the hprt locus were observed. our results indicate that transformation by bglii n is independent from the induction of somatic mutations and su ... | 1989 | 2539146 |
| promoting effect of croton oil on the induction of cervical and vaginal cancers with herpes simplex virus types 1 and 2 in mice. | hybrid swiss virgin mice (kuan-min strain) were challenged intravaginally with uv-inactivated herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) twice a week for 16 times and subsequently with croton oil or control medium twice a week for 27 times. after a period of 180 days the exposure to hsv-2 plus control medium induced premalignant and malignant lesions of the cervix and vagina in 50.0% of the mice. the exposure to hsv-2 plus croton oil induced similar lesions in 78.2% of the mice. the e ... | 1989 | 2539313 |
| search for human papillomavirus, herpes simplex virus and c-myc oncogene in human genital tumors. | we tested grade iii cervical intra-epithelial neoplasms (cin iii), genital invasive carcinomas and healthy genital tissues for the presence of a number of viral and cellular parameters, considered to be risk factors in genital oncogenesis. the results show that: (i) about 30% of genital tumors had homology to hsv-2 bglii n and/or bamhi e dna fragments; (2) positivity to hsv-2-specific protein icp10, encoded by sequences within the hsv-2 bamhi e dna fragment, was detected in 29/55 neoplastic geni ... | 1989 | 2539328 |
| short, duplicated sequence indicative of the recombinogenicity of the junction between a unique and an inverted repeat sequence in the s component of the herpes simplex virus type 1 genome. | a herpes simplex virus type 1 (hsv-1) strain, b3, was found to have a short duplication on the left junction between the unique sequence (us) and the inverted repeat sequence (rs) in the s component of the genome dna. a short region of rs contiguous to the left us-rs junction was duplicated in b3. based on the nucleotide sequences in and around the us-rs junctions of b3 and other hsv-1 strains, a concept of junction stretch was proposed. the organization of junction stretch is rs side 5'-(g or a ... | 1989 | 2539490 |
| a neutrophil-derived antiviral protein: induction requirements and biological properties. | polymorphonuclear neutrophilic granulocytes (pmn) have been implicated as playing a role in antiviral defense. in addition to having phagocytic and cytotoxic activities, pmn may produce an antiviral substance with interferon (ifn)-like activity. the product, for which the name polyferon (pf) has been coined, is produced upon direct encounter of pmn with bovine herpesvirus 1 (bhv-1)-infected bovine cells or membranes thereof. exposure to purified virus only does not induce pf. the intimate intera ... | 1989 | 2539494 |
| herpes simplex virus type 2 mutants with deletions in the intergenic region between icp4 and icp22/47: identification of nonessential cis-acting elements in the context of the viral genome. | in herpes simplex virus type 2, the mrnas of icp4 and icp22/47 are divergently transcribed and their transcription initiation sites are separated by 750 base pairs (l. j. whitton and j. b. clements, nucleic acids res. 12:2061-2078, 1984). this 750-base-pair region contains many recognized cis-acting elements, including two tata boxes, numerous sp1-binding sites, four taatgarat motifs, at least one icp4-binding site, and two origins of replication (oris) linked in tandem. in this report, we descr ... | 1989 | 2539500 |
| possible transmission of herpes simplex virus by organ transplantation. | herpes simplex virus commonly reactivates in seropositive transplant recipients but has not been generally thought to be transmissible by the transplanted organ itself. we studied two consecutive cases of disseminated hsv, without mucosal lesions, occurring in a heart and in a pancreatic transplant recipient, and implicate the allografts as the source of the virus. in both cases the recipients were seronegative pretransplant by complement fixation (less than 1:4), neutralization (less than 1:2), ... | 1989 | 2539664 |
| a prospective study of the effects of acyclovir treatment on the hsv-2 lymphoproliferative response of persons with frequently recurring hsv-2 genital infections. | in a randomized double-blinded fashion, herpes simplex virus type 2 specific lymphocyte proliferation (hsv-2-lp) was studied in patients with frequently recurring genital hsv-2 who were administered daily suppressive oral acyclovir (s-acv) or placebo and acyclovir for 5 d (plc/acv) with recurrences. the pretreatment hsv-2-lp of subjects was low compared with that of controls with infrequently recurring genital hsv-2 (54,000 vs. 110,000 cpm). the hsv-2-lp increased with both s-acv and plc/acv (10 ... | 1989 | 2540245 |
| development of clinically recognizable genital lesions among women previously identified as having "asymptomatic" herpes simplex virus type 2 infection. | to determine if patients initially identified by history, clinical examination, and serologic status as having asymptomatic herpes simplex virus type 2 infection report clinically recognizable genital lesions after having a detailed instructional session on the clinical signs and symptoms of genital herpes. | 1989 | 2541645 |
| analysis of unselected hsv-1 mckrae/hsv-2 hg 52 recombinants demonstrates preferential recombination between intact genomes and restriction endonuclease fragments containing an origin of replication. | to identify viral genes involved in reactivation of herpes simplex virus from latency, intertypic hsv-1 strain mckrae/hsv-2 strain hg 52 recombinants were selected following cotransfection of intact mckrae dna and xbai or hpai cleaved hg 52 dna. eleven separately obtained recombinants containing hg 52 inserts between 0.35-0.56 and/or 0.82-1.0 map units (mu) were isolated. it was noted that with hpai digested hg 52 dna, only recombinants containing type 2 inserts from hpai d (0.35-0.57) and/or co ... | 1989 | 2541672 |
| two colour dna in situ hybridization for the detection of two viral genomes using non-radioactive probes. | methods for the simultaneous detection of two virus types in cytological preparations or tissue sections by non-radioactive in situ hybridization were investigated. as a model system, caski cells, which have human papilloma virus type 16 (hpv16) dna integrated in their cellular genome, were in vitro infected with herpes simplex virus 2 (hsv2). dna probes for both viruses were labeled with biotin, acetylaminofluorene (aaf), and transaminated-sulfonated cytosine (ts-modified). best results were ob ... | 1989 | 2542189 |
| serum iga antibodies in hsv asymptomatic genital infections. | cervico-vaginal swabs and serum samples from 81 women without herpes-associated clinical symptoms were examined for virus isolation and for the presence of iga antibodies to herpes simplex virus (hsv) using the indirect fluorescent antibody technique. nineteen of the 81 women were followed up for 2 years. blood samples and swabs from cutaneous lesions were obtained from another group of 20 women (medical staff) with herpes labialis. by analysing the specific anti-hsv iga antibodies, a higher geo ... | 1989 | 2542431 |
| identification of immediate-early-type cis-response elements in the promoter for the ribonucleotide reductase large subunit from herpes simplex virus type 2. | regulation of the expression of the herpes simplex virus (hsv) type 2 large subunit of ribonucleotide reductase (icp10) gene was studied directly by immunofluorescence or by chloramphenicol acetyltransferase analysis with hybrid icp10 promoter constructions. in vero cells, cotransfection with dna encoding hsv ie110 or vmw65 proteins or hcmv ie2 enhanced expression at least 10-fold. in contrast, expression was minimally enhanced by dna encoding ie175 at low doses and slightly reduced at high dose ... | 1989 | 2542589 |
| antigen-presenting liposomes are effective in treatment of recurrent herpes simplex virus genitalis in guinea pigs. | the therapeutic and immunologic effects of a liposome preparation containing both a macrophage activator, muramyl-tripeptide-phosphatidylethanolamine, and a recombinant antigen, glycoprotein d of herpes simplex virus type 1, have been investigated. this preparation was tested in vitro for the ability to stimulate peripheral blood lymphocytes and in vivo for the control of recurrent herpes genitalis in guinea pigs. our results show that the liposome-antigen-adjuvant preparation is capable of enha ... | 1989 | 2542605 |
| transmission of genital herpes by donor insemination. | this report describes a donor in a therapeutic donor insemination program who asymptomatically acquired a primary herpes simplex virus type 2 (hsv-2) infection from his long-standing sexual partner. his fresh semen was used to inseminate two hsv-seronegative recipients; in one a primary hsv-2 infection developed, and in one it did not. direct evidence of transmission from donor to recipient was documented by restriction enzyme analysis of the hsv-2 isolates obtained from the donor's semen and fr ... | 1989 | 2542632 |
| [structure and functional analysis of genomes of herpesviruses: herpes simplex virus type 1 and type 2 (hsv-1, hsv-2)]. | 1989 | 2542648 | |
| [chemotherapy to thymidine kinase producing virus (hsv-1, hsv-2, vzv) infection]. | 1989 | 2542661 | |
| anti-herpes simplex virus activity of 5-substituted 2-pyrimidinone nucleosides. | several 5-substituted 2-pyrimidinone 2'-deoxyribonucleoside (pdr) analogs were examined for their anti-herpes simplex virus (hsv) activity in cell culture. the order of potency of their antiviral activities against hsv type 1 (hsv-1) and hsv-2 was iodo pdr approximately ethynyl pdr approximately propynyl pdr. the antiviral action of iodo pdr is dependent on the ability of hsv to induce virus-specified thymidine kinase in infected cells. several hsv-1 variants with altered thymidine kinase change ... | 1989 | 2543279 |
| a variant of herpes simplex virus type 2 strain hg52 with a 1.5 kb deletion in rl between 0 to 0.02 and 0.81 to 0.83 map units is non-neurovirulent for mice. | the virulence of a deletion variant of herpes simplex virus type 2 (hsv-2) strain hg52 has been determined by intracranial inoculation of 3-week-old balb/c mice. the variant jh2604 has a 1.5 kb deletion within each copy of the long repeat region (rl) of the genome between 0 to 0.02 and 0.81 to 0.83 map units. jh2604 is avirulent for mice compared to the parental wild-type virus, and fails to replicate in mouse brain in vivo. correction of the deletion by marker rescue resulted in the isolation o ... | 1989 | 2543755 |
| mild acidic ph inhibition of the major pathway of herpes simplex virus entry into hep-2 cells. | penetration of the kos strain of herpes simplex virus type 1 (hsv-1) and the ms and 333 strains of herpes simplex virus type 2 (hsv-2) into hep-2 cells at ph 6.3 was at least 100-fold less efficient than at ph 7.4. penetration of two low passage clinical isolates was completely blocked at ph 6.3. the syncytium-forming hsv-1 strains gc and mp were less sensitive than kos to the mild acidic conditions. the inhibition was completely reversed upon neutralization of the medium. penetration was assaye ... | 1989 | 2543762 |
| herpes simplex virus type 2 primes mouse macrophages for an early and genetically determined respiratory burst mediated by interferon-alpha/beta. | the influence of infection by herpes simplex virus type 2 (hsv-2) on the respiratory burst capacity of mouse macrophages was studied by luminol-dependent chemiluminescence with phorbol myristate acetate (pma) as trigger. peritoneal cells from virus-infected mice were strongly primed for a respiratory burst during the acute phase of the infection. by 12 h after infection the response had increased 40-fold over control values. most of the response was elicited by mononuclear phagocytes. when resti ... | 1989 | 2543784 |
| a seroepidemiologic survey of the prevalence of herpes simplex virus type 2 infection in the united states. | the prevalence of infection with the genital herpes simplex virus type 2 (hsv-2) has been difficult to ascertain, primarily because of the large percentage of subclinical cases and the limitations in specificity of serologic assays for antibody to hsv-2. to obtain an improved estimate of the distribution of hsv-2 infection in the united states, we used an hsv type-specific antibody assay to test serum samples from 4201 participants in the second national health and nutrition examination survey. ... | 1989 | 2543928 |
| induction of cervical neoplasia in the mouse by herpes simplex virus type 2 dna. | induction of cervical neoplasia in the mouse cervix by herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) has been reported. the present study was done to determine if transfection with dna of hsv-2 can induce carcinogenesis in this animal model. genomic hsv-2 dna was isolated from infected hep-2 cells and separated from host cell dna by cesium chloride density gradient centrifugation. the dna was applied to mouse cervix for periods of 80-100 weeks. experimental controls were treated with uninfe ... | 1989 | 2543981 |
| effect of phosphorothioate homo-oligodeoxynucleotides on herpes simplex virus type 2-induced dna polymerase. | effects of phosphorothioate oligodeoxynucleotides of different chain length and base composition on herpes simplex virus (hsv) type 2 (strain 333)-induced dna polymerase have been examined in vitro. the anti-hsv-2 dna polymerase activity was related to the base composition of the analogs, with the order of potency: deoxycytidine greater than thymidine greater than deoxyadenosine, for compounds with equal chain length. the potency was also related to oligomer chain length, since it was observed t ... | 1989 | 2544598 |
| viral antigen stimulation of the production of human monokines capable of regulating hiv1 expression. | we have previously described model systems for cytokine-induced regulation of chronically hiv-infected promonocyte and t cell clones. using these systems, we have shown that monokines contained in supernatants from lps-stimulated human monocyte/macrophages (mo) up-regulate hiv expression, reflected by an increase in reverse transcriptase activity, viral rna levels, and expressed viral proteins. current studies were designed to determine whether viral ag can interact with mo and secondarily affec ... | 1989 | 2544645 |
| in vitro evaluations of condoms with and without nonoxynol 9 as physical and chemical barriers against chlamydia trachomatis, herpes simplex virus type 2, and human immunodeficiency virus. | simulated in vitro intercourse conditions demonstrated that unlubricated latex condoms provide an effective physical barrier to high concentrations of chlamydia trachomatis, herpes simplex virus type 2, and human immunodeficiency virus. however, since condoms can be damaged after manufacturing inspection and prior to use, latex condoms alone should not be perceived as absolute protection against stds. nonoxynol 9 used in conjunction with condoms provided additional, yet still not foolproof, prot ... | 1989 | 2545003 |
| vero cells co-infected with chlamydia trachomatis and herpes simplex virus type 2: a scanning and transmission electron microscope study. | vero (african green monkey kidney) cells infected with chlamydia trachomatis (serovar l2) (ct-l2) for 48 hr and superinfected with herpes simplex virus type 2 (hsv-2) were fixed 24 hr later and examined with the transmission electron microscope (tem) and scanning electron microscope (sem). ultrastructural examination of the co-infected cells showed that, although many ct-l2 inclusions were present, most were empty of reticulate bodies or elementary bodies. however, large numbers of viruses were ... | 1989 | 2545004 |
| herpes simplex virus glycoprotein immunotherapy of recurrent genital herpes: factors influencing efficacy. | recombinant herpes simplex virus type 1 (hsv-1) glycoproteins b (gb) and d (gd) were used as immunotherapeutic agents for the treatment of recurrent genital herpes in guinea pigs. administration of a gbgd vaccine eight to 21 days after intravaginal hsv-2 inoculation significantly increased the titer of anti-hsv antibodies (p less than 0.005) while significantly reducing the frequency of subsequent herpetic recurrences (p less than 0.05). the effectiveness of gbgd immunotherapy was influenced by ... | 1989 | 2545166 |
| herpes simplex virus type 2 infection of unstimulated human t-lymphocytes. | unstimulated human leukemia t-cell lines (molt-4, mt-4) were tested for their susceptibility to herpes simplex virus type 2 (hsv-2) infection. permissive infection of mt-4 cells was demonstrated by growth curve and infectious center assays. in growth curve experiments new progeny virus replication was detected by 24 hrs and maximum titers of hsv-2 replication were measured by 72 hrs after infection of mt-4 cells, whereas, molt-4 cells did not produce detectable infectious hsv-2 in growth curve e ... | 1989 | 2545223 |
| rapid detection of herpes simplex virus with fluorescein-labeled helix pomatia lectin. | the use of fluorescein-conjugated helix pomatia lectin was shown to be as effective as fluorescein-conjugated monoclonal antibody reagents for the detection and differentiation of herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) in mrc-5 cell culture. cells infected with hsv-1 generally displayed a pattern of nongranular or diffuse fluorescence, while cells infected with hsv-2 were identified by the production of fluorescent grains and flecks. this unique nonimmunological reagent, when u ... | 1989 | 2545739 |
| protein kinase activity associated with the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10). | the large subunit of the herpes simplex virus type 2 (hsv-2) ribonucleotide reductase (rr1) is demonstrated to possess serine/threonine-specific kinase activity. computer-assisted sequence analysis identified regions within the amino terminus of icp10 that are homologous to the catalytic domain of known protein kinases (pks). an in vitro kinase assay confirmed the ability of icp10, immunoprecipitated from either hsv-2-infected cells or from cells transfected with an icp10 expression vector, to a ... | 1989 | 2545912 |
| herpes simplex virus type 1 immediate-early protein vmw110 reactivates latent herpes simplex virus type 2 in an in vitro latency system. | reactivation of latent herpes simplex virus type 2 (hsv-2) by the immediate-early protein vmw110 was studied by using an in vitro latency system. adenovirus recombinants that express vmw110 reactivated latent hsv-2. an hsv-1 mutant possessing a deletion in a carboxy-terminal region of vmw110 reactivated latent hsv-2, whereas mutant fxe, which has a deletion in the second exon, did not. therefore, vmw110 alone is required to reactivate latent hsv-2 in vitro, and the region of vmw110 defined by th ... | 1989 | 2545921 |
| immunogenicity of herpes simplex virus glycoprotein gb-1-related protein produced in yeast. | a protein related to glycoprotein b of herpes simplex virus type 1 (hsv-1) produced in yeast (ygb-1) was purified with an immunoadsorbent. the molecular weight of the purified ygb-1 as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis was 96,000. mice injected twice with ygb-1 adsorbed to alum developed elisa antibody to ygb-1, neutralizing antibody to hsv-1 and a lymphoproliferative response to ygb-1 and hsv-1. the immunized mice were protected against intraperitoneal and ... | 1989 | 2546329 |
| sexual activity, contraception, genital infections, and cervical cancer: support for a sexually transmitted disease hypothesis. | a case-control study was conducted in utah between 1984 and 1987 to examine risk factors for cervical cancer. interviews were completed with 266 histologically confirmed carcinoma in situ and invasive squamous cell cervical cancer cases who were categorically matched by age to 408 controls. among the factors identified as altering risk for cervical cancer, after adjustment for age, education, church attendance, and cigarette smoking, were: having numerous sex partners (odds ratio (or) = 8.99 for ... | 1989 | 2546422 |
| inhibition of herpes simplex virus infection by pine cone antitumor substances. | several antitumor substances extracted from cones of various pine trees inhibited the plaque formation of herpes simplex virus types 1 and 2 (hsv-1, hsv-2) strains in african green monkey kidney cells and human adenocarcinoma cells. the 50% effective dose of the most active fraction, fr. vi (0.3 micrograms/ml) was 0.001 times its 50% cytotoxic dose (greater than 300 micrograms/ml). the anti-hsv activity of various pine cone extracts increased with their acidity. identification of the polyphenol ... | 1989 | 2546481 |
| biological and biochemical characterization of clinical isolates of herpes simplex virus type 2 resistant to acyclovir. | a series of clinical isolates of herpes simplex virus type 2 were taken from a patient with chronic lymphocytic leukemia. acyclovir (acv) susceptibility assays revealed that some isolates were resistant to acv and cross-resistant to ganciclovir but not to phosphonoacetic acid. the nature of the resistance was examined further. a number of cloned variants were generated, and thymidine kinase and dna polymerase assays were carried out. variants that were resistant to acv were found to be thymidine ... | 1989 | 2546486 |
| [neutralizing activity against herpes simplex in maternal milk]. | human milk could play a role in the protection of the newborn from herpes simplex virus 2 contamination. neutralizing activity against herpes simplex virus 2 was measured in 94 human milk samples and in 31 sera from healthy women. a weak neutralizing activity was detected in 48 milk samples. they were neither correlated to anti-herpes simplex virus antibodies found in milk, nor to the neutralizing activity in sera. the origin and non immunologic nature of this activity are discussed. | 1989 | 2546513 |
| serological evidence for variation in the incidence of herpesvirus infections in different species of apes. | sera from captive lowland gorillas, chimpanzees, orangutans, and gibbons were screened by enzyme-linked immunosorbent assay (elisa) for antibody to herpesviruses serologically related to human herpes simplex virus types 1 and 2 (hsv-1, hsv-2), a baboon virus (sa8), and a macaque herpesvirus (b virus). the incidence of herpesvirus antibodies varied considerably among the different species, gorillas having the highest incidence of seropositivity (65.4%) and orangutans the lowest. the virus specifi ... | 1989 | 2546978 |
| synthesis and antiviral activity of the enantiomeric forms of carba-5-iodo-2'-deoxyuridine and carba-(e)-5-(2-bromovinyl)-2'-deoxyuridine. | both enantiomers of the carbocyclic analogues of 5-iodo-2'-deoxyuridine (14 and ent-14) and of (e)-5-(2-bromo-vinyl)-2'-deoxyuridine (16 and ent-16) were synthesized by using (+)- or (-)-endo-norborn-5-en-2-yl acetate or butyrate, respectively, as starting materials. against herpes simplex virus type 1 (+)-c-bvdu (16) was only slightly less active than bvdu itself, whereas (-)-c-bvdu (ent-16) proved to be 10-400-fold less effective, depending on the strain investigated. against hsv-2 both (+)- a ... | 1989 | 2547072 |
| smoking and infectious agents and risk of in situ cervical cancer in sydney, australia. | in a study of 116 in situ cervical cancer patients and 193 matched community controls in sydney, australia, smoking was found to be a major risk factor. current smokers had a adjusted relative risk [rr] of 4.5 compared to nonsmokers [95% confidence interval (ci) 2.2-9.1] and exsmokers a rr of 1.3 [95% ci 0.6-3.0]. there was a stepwise dose-response relationship between risk and number of cigarettes smoked (30+ cigarettes/day, rr = 5.1, 95% ci 1.5-17.3); this dose-response relationship was more m ... | 1989 | 2547521 |