Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| molecular characterization of novel sulfotransferases from the tick, ixodes scapularis. | ixodes scapularis, commonly known as the blacklegged or deer tick, is the main vector of lyme disease in the united states. recent progress in transcriptome research has uncovered hundreds of different proteins expressed in the salivary glands of hard ticks, the majority of which have no known function, and include many novel protein families. we recently identified transcripts coding for two putative cytosolic sulfotransferases in these ticks which recognized phenolic monoamines as their substr ... | 2011 | 21708020 |
| strategic control of cattle ticks: milk producers' perceptions. | in order to evaluate milk producers' knowledge about tests on tick sensitivity to acaricides, efficiency tests and strategic control, questionnaires were delivered to 670 producers. from these, 163 responses were received. these producers had sent ingurgitated female ticks to be subjected to tests on tick sensitivity to acaricides at embrapa between 2001 and 2005. most of the completed questionnaires came from the state of minas gerais, brazil. after descriptive analysis and correlation of varia ... | 2011 | 21722490 |
| isolation and characterisation of acaricidal and larvicidal novel compound (2s,5r,6r)-2-hydroxy-3,5,6-trimethyloctan-4-one from streptomyces sp. against blood-sucking parasites. | the aim of the present study was to assess the acaricidal and larvicidal property of marine actinobacterial compound (2s,5r,6r)-2-hydroxy-3,5,6-trimethyloctan-4-one extracted and isolated from streptomyces sp. vitddk3 tested against the larvae of rhipicephalus (boophilus) microplus canestrini (acari: ixodidae), anopheles subpictus grassi and culex quinquefasciatus say (diptera: culicidae). the isolate was taxonomically characterised, identified and designated as streptomyces sp. vitddk3. the cru ... | 2011 | 21698506 |
| evolutionary conserved micrornas are ubiquitously expressed compared to tick-specific mirnas in the cattle tick rhipicephalus (boophilus) microplus. | micrornas (mirnas) are small non-coding rnas that act as regulators of gene expression in eukaryotes modulating a large diversity of biological processes. the discovery of mirnas has provided new opportunities to understand the biology of a number of species. the cattle tick, rhipicephalus (boophilus) microplus, causes significant economic losses in cattle production worldwide and this drives us to further understand their biology so that effective control measures can be developed. to be able t ... | 2011 | 21699734 |
| attenuation of virulence in an apicomplexan hemoparasite results in reduced genome diversity at the population level. | abstract: | 2011 | 21838895 |
| gene expression evidence for off-target effects caused by rna interference-mediated gene silencing of ubiquitin-63e in the cattle tick rhipicephalus microplus. | knowledge of cattle tick (rhipicephalus (boophilus) microplus; acari: ixodidae) molecular and cellular pathways has been hampered by the lack of an annotated genome. in addition, most of the tick expressed sequence tags (ests) available to date consist of ôê+50% unassigned sequences without predicted functions. the most common approach to address this has been the application of rna interference (rnai) methods to investigate genes and their pathways. this approach has been widely adopted in tick ... | 2011 | 21712043 |
| an elisa to detect serum antibodies to the salivary gland toxin of ixodes holocyclus neumann in dogs and rodents. | the ixodes holocyclus tick causes paralysis in up to 10,000 companion and domestic animals each year in australia. treatment requires the removal of the parasite and the administration of a commercial tick antiserum that is prepared from hyperimmune dogs. each batch of this serum is initially tested for toxin-neutralising potency in a mouse bioassay that is expensive, time consuming, and subjective. with the aim of developing a rapid in vitro assay to replace the bioassay, we used a partially pu ... | 2011 | 21687655 |
| antigen fingerprinting of polyclonal antibodies raised in immunized chickens with tick total proteins: a reservoir for the discovery of novel antigens. | identification of tick-protective antigens remains the limiting step in vaccine development. the authors have generated several b cell epitope candidates by fingerprinting rhipicephalus (boophilus) microplus proteins that were characterized through bioselection of random peptide phage display libraries against polyclonal antibodies antitick proteins. from 280 clones selected and sequenced, 107 distinct reactive clones were validated by dot-blot assays. eight consensus motifs were generated, and ... | 2011 | 21844329 |
| rhipicephalus (boophilus) microplus: expression and characterization of bm86-cg in pichia pastoris. | the cattle tick rhipicephalus (boophilus) microplus is responsible for great economic losses. it is mainly controlled chemically, with limitations regarding development of resistance to the chemicals. vaccines may help control this parasite, thereby reducing tick pesticide use. in this light, we performed subcloning of the gene of the protein bm86-gc, the homologue protein that currently forms the basis of vaccines (gavac(tm) and tickgard(plus)) that have been developed against cattle ticks. the ... | 2011 | 21722483 |
| a new in vitro test to evaluate the resistance level against acaricides of the cattle tick, rhipicephalus (boophilus) microplus. | in this article we present a new bioassay to assess the resistance status of ticks to acaricides. the larval tarsal test (ltt) is a sensitive, highly time-effective in vitro test. it allows the investigation of a large number of compounds and doses on the cattle tick rhipicephalus (boophilus) microplus in a short period of time. the ability of the ltt to assess the lethal concentration at 50% mortality (lc(50)) and resistance ratios (rrs) of a susceptible and a resistant r. microplus strain was ... | 2011 | 21741175 |
| An intensive search for promising fungal biological control agents of ticks, particularly Rhipicephalus microplus. | Entomopathogenic fungi have been investigated worldwide as promising biological control agents of the cattle tick Rhipicephalus microplus. The current study evaluates the virulence of several fungal isolates to R. microplus larva in the laboratory as part of an effort to identify isolates with promise for effective biocontrol of R. microplus in the field. Sixty fungal isolates, encompassing 5 Beauveria spp. and 1 Engyodontium albus (=Beauveria alba), were included in this study. In addition to b ... | 2011 | 21705145 |
| Scabies mite peritrophins are potential targets of human host innate immunity. | Pruritic scabies lesions caused by Sarcoptes scabiei burrowing in the stratum corneum of human skin facilitate opportunistic bacterial infections. Emerging resistance to current therapeutics emphasizes the need to identify novel targets for protective intervention. We have characterized several protein families located in the mite gut as crucial factors for host-parasite interactions. Among these multiple proteins inhibit human complement, presumably to avoid complement-mediated damage of gut ep ... | 2011 | 21980545 |
| Acaricidal effect of essential oils from Lippia graveolens (Lamiales: Verbenaceae), Rosmarinus officinalis (Lamiales: Lamiaceae), and Allium sativum (Liliales: Liliaceae) against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). | Acaricidal effects of three essential oils extracted from Mexican oregano leaves (Lippia graveolens Kunth), rosemary leaves (Rosmarinus officinalis L.), and garlic bulbs (Allium sativum L.) on 10-d-old Rhipicephalus (Boophilus) microplus (Canestrini) tick larvae were evaluated by using the larval packet test bioassay. Serial dilutions of the three essential oils were tested from a starting concentration of 20 to 1.25%. Results showed that both Mexican oregano and garlic essential oils had very s ... | 2011 | 21845941 |
| Acaricidal, insecticidal, and larvicidal efficacy of fruit peel aqueous extract of Annona squamosa and its compounds against blood-feeding parasites. | Plant products may be alternative sources of parasitic control agents, since they constitute a rich source of bioactive compounds that are eco-friendly and nontoxic products. The plant extracts are good and safe alternatives due to their low toxicity to mammals and easy biodegradability. In the present study, fruit peel aqueous extract of Annona squamosa (Annonaceae) extracted by immersion method exhibited adulticidal activity against Haemaphysalis bispinosa (Acarina: Ixodidae) and the hematopha ... | 2011 | 22006187 |
| Effects of microplusin, a copper-chelating antimicrobial peptide, against Cryptococcus neoformans. | Microplusin is an antimicrobial peptide isolated from the cattle tick Rhipicephalus (Boophilus) microplus. Its copper-chelating ability is putatively responsible for its bacteriostatic activity against Micrococcus luteus as microplusin inhibits respiration in this species, which is a copper-dependent process. Microplusin is also active against Cryptococcus neoformans (MIC(50) = 0.09 µM), the etiologic agent of cryptococcosis. Here, we show that microplusin is fungistatic to C. neoformans and thi ... | 2011 | 22092765 |
| The effects of flumethrin (Bayticol® pour-on) on European ticks exposed to treated hairs of cattle and sheep. | Tick infestations in cattle and sheep pose serious health problems when agents of diseases are transmitted. In addition, blood feeding of ticks induces enormous economic losses due to reduced weight gain of infested animals. The present study was designed to investigate the effects of exposure to hairs clipped from cattle and sheep treated with flumethrin (Bayticol®) on European ticks. The dose used was 10 ml/100 kg body weight for both animal species. At intervals of 7 days (days 7, 14, 21, 28 ... | 2011 | 22205348 |
| distinctive amino acid composition profiles in salivary proteins of the tick ixodes scapularis. | bioinformatic analysis of the amino acid composition of proteins of the tick ixodes scapularis showed that, in comparison to other secreted proteins, salivary proteins in general have higher frequencies of polar residues and lower frequencies of the non-polar residues leucine and valine. computer prediction of linear b-cell epitopes showed that polar residues were associated with the presence of high-quality epitopes and that tick salivary proteins included significantly more proteins with predi ... | 2011 | 22108016 |
| An insight into the sialotranscriptome and proteome of the coarse bontlegged tick, Hyalomma marginatum rufipes. | Ticks are mites specialized in acquiring blood from vertebrates as their sole source of food and are important disease vectors to humans and animals. Among the specializations required for this peculiar diet, ticks evolved a sophisticated salivary potion that can disarm their host's hemostasis, inflammation, and immune reactions. Previous transcriptome analysis of tick salivary proteins has revealed many new protein families indicative of fast evolution, possibly due to host immune pressure. The ... | 2011 | 21851864 |
| Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) HP88 for biological control of Rhipicephalus microplus (Acari: Ixodidae): The effect of different exposure times of engorged females to the nematodes. | The aim of this study was to assess the influence of different exposure times of engorged female the Rhipicephalus microplus to infective juveniles of Heterorhabditis bacteriophora isolate HP88. The engorged females were divided into seven groups (six treatments and one control) of 20 ticks each with statistically similar average weights (p>0.05) and exposed to concentrations of 300 nematodes/tick for periods of 3, 6, 12, 24, 48 and 72h. The following biological parameters were monitored: pre-ov ... | 2011 | 22093907 |
| applicability of in vitro bioassays for the diagnosis of ivermectin resistance in rhipicephalus microplus (acari: ixodidae). | the applicability of laboratory bioassays to diagnose ivermectin (ivm) resistance in rhipicephalus microplus was evaluated. adult immersion tests (aits), larval immersion tests (lits) and larval packet tests (lpts) were performed to characterise the effects of ivermectin toxicity on adults and larvae of a susceptible reference strain. the ait was determined to be a reasonable assay but requires a large number of individuals to attain interpretable results. the lit and lpt were validated with an ... | 2011 | 21978742 |
| CattleTickBase: An integrated Internet-based bioinformatics resource for Rhipicephalus (Boophilus) microplus. | The Rhipicephalus microplus genome is large and complex in structure, making it difficult to assemble a genome sequence and costly to resource the required bioinformatics. In light of this, a consortium of international collaborators was formed to pool resources to begin sequencing this genome. We have acquired and assembled genomic DNA into contigs that represent over 1.8Gigabase pairs of DNA from gene-enriched regions of the R. microplus genome. We also have several datasets containing transcr ... | 2011 | 22178513 |
| Cutaneous hypersensitivity test to evaluate phage display anti-tick borne vaccine antigen candidates. | Early experiments performed by our group with the phage display technique revealed the potential for using epitope-displaying phages (mimotopes) as a tool for tick antigen discovery. Thus, as a preliminary study, inflammatory reactions induced by phage display tick-borne candidates were investigated by using the cutaneous hypersensitivity test. The profile of selected Rhipicephalus microplus mimotopes was assessed on tick field-exposed cattle and our data indicated a pattern similar to immediate ... | 2011 | 21945847 |
| a further insight into the sialome of the tropical bont tick, amblyomma variegatum. | ticks--vectors of medical and veterinary importance--are themselves also significant pests. tick salivary proteins are the result of adaptation to blood feeding and contain inhibitors of blood clotting, platelet aggregation, and angiogenesis, as well as vasodilators and immunomodulators. a previous analysis of the sialotranscriptome (from the greek sialo, saliva) of amblyomma variegatum is revisited in light of recent advances in tick sialomes and provides a database to perform a proteomic study ... | 2011 | 21362191 |
| structural and biochemical characterization of a recombinant triosephosphate isomerase from rhipicephalus (boophilus) microplus. | triosephosphate isomerase (tim) is an enzyme with a role in glycolysis and gluconeogenesis by catalyzing the interconversion between glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. this enzyme has been used as a target in endoparasite drug development. in this work we cloned, expressed, purified and studied kinetic and structural characteristics of tim from tick embryos, rhipicephalus (boophilus) microplus (bmtim). the km and vmax of the recombinant bmtim with glyceraldehyde 3-phospha ... | 2011 | 21396445 |
| acaricidal properties of the essential oil and precocene ii obtained from calea serrata (asteraceae) on the cattle tick rhipicephalus (boophilus) microplus (acari: ixodidae). | calea serrata less. (asteraceae), an endemic species of south brazil known as "quebra-tudo", is used in afro-brazilian religious rituals and in folk medicine for treating liver disorders. phytochemical studies of the n-hexane extract of this plant demonstrated the presence of precocene ii, a benzopyran derivative known for its insecticidal activity. the aim of this work was to isolate this benzopyran and determine the chemical composition of the essential oil of c. serrata and further to evaluat ... | 2011 | 21402447 |
| acaricidal activity of palicourea marcgravii, a species from the amazon forest, on cattle tick rhipicephalus (boophilus) microplus. | leaves of palicourea marcgravii were extracted successively with hexane, ethyl acetate and ethanol in order to evaluate their acaricidal activity on larvae and adult stages of the cattle tick rhipicephalus (boophilus) microplus. the ethyl acetate extract showed the highest bioactivity of the tested extracts, which contained 0.12% monofluoroacetic acid. on engorged female, the ethyl acetate extract showed a lethal concentration 50% - lc(50)=30.08 mg ml(-1), inhibitory concentration 50% - ic(50)=5 ... | 2011 | 21411227 |
| colonization of grande comore island by a lineage of rhipicephalus appendiculatus ticks. | union of the comoros suffered a severe east coast fever epidemic in 2004. rhipicephalus appendiculatus was probably involved in pathogen transmission as this competent tick species, although previously absent from comoros, was sampled on 4 animals on one geographical site during the epidemic. we carried out an entomological survey on all three islands of union of the comoros to establish cattle tick species distribution with a special emphasis on r. appendiculatus. we investigated r. appendicula ... | 2011 | 21414194 |
| an important role of a pyrethroid-sensing residue f1519 in the action of the n-alkylamide insecticide btg 502 on the cockroach sodium channel. | deltamethrin, a pyrethroid insecticide, and btg 502, an alkylamide insecticide, target voltage-gated sodium channels. deltamethrin binds to a unique receptor site and causes prolonged opening of sodium channels by inhibiting deactivation and inactivation. previous (22)na(+) influx and receptor binding assays using mouse brain synaptoneurosomes showed that btg 502 antagonized the binding and action of batrachotoxin (btx), a site 2 sodium channel neurotoxin. however, the effect of btg 502 has not ... | 2011 | 21426938 |
| phenotype changes inherited by crossing pyrethroid susceptible and resistant genotypes from the cattle tick riphicephalus (boophilus) microplus. | dialelic crosses and backcrosses of pyrethroid resistant (rr) and susceptible (ss) rhipicephalus (boophilus) microplus tick strains were carried out and the substitution (phe-ile) within the sodium channel gene was monitored in order to analyze the effects of the genotype on the pyrethroid resistance phenotype as measured by the larval packet test (lpt). parental strains: susceptible (ss) and resistant (rr); dialelic crosses: rs (?rr × ?ss), and sr (?ss × ?rr); and backcrosses: rs × ss, rs × rr, ... | 2011 | 21431928 |
| selective control of rhipicephalus (boophilus) microplus in fipronil-treated cattle raised on natural pastures in lages, state of santa catarina, brazil. | an examination of a selective control of rhipicephalus (boophilus) microplus and consequent cost reduction was carried out in two areas of native pasture in the municipality of lages, state of santa catarina, southern brazil, from may 2007 to april 2009. forty cattle were divided into two groups of 20 animals each (conventional and selective control groups). at 14-day intervals female ticks larger than 4.5 mm found on the right flank of cattle bodies were counted, and these results multiplied by ... | 2011 | 21439225 |
| in vitro activity of artemisia annua l (asteraceae) extracts against rhipicephalus (boophilus) microplus. | the activity of plant extracts on parasites may indicate groups of substances that are potentially useful for controlling rhipicephalus (boophilus) microplus. the aim of the present study was to investigate the in vitro action of artemisia annua extracts on this tick. the concentrations of the sesquiterpene lactones artemisinin and deoxyartemisinin present in plant extracts were quantified via high-performance liquid chromatography. four extracts produced from the concentrated crude extract (cce ... | 2011 | 21439229 |
| retraction. detection and identification of ehrlichia species in rhipicephalus (boophilus) microplus ticks in cattle from xiamen, china. | 2011 | 21443413 | |
| bacillus sp. cdb3 isolated from cattle dip-sites possesses two ars gene clusters. | contamination of soil and water by arsenic is a global problem. in australia, the dipping of cattle in arsenic-containing solution to control cattle ticks in last centenary has left many sites heavily contaminated with arsenic and other toxicants. we had previously isolated five soil bacterial strains (cdb1-5) highly resistant to arsenic. to understand the resistance mechanism, molecular studies have been carried out. two chromosome-encoded arsenic resistance (ars) gene clusters have been cloned ... | 2011 | 21476346 |
| efficacy and blood sera analysis of a long-acting formulation of moxidectin against rhipicephalus (boophilus) microplus (acari: ixodidae) on treated cattle. | the therapeutic and persistent efficacy of a single subcutaneous injection of a long-acting formulation of moxidectin at a concentration of 1 mg/kg body weight was determined against rhipicephalus (boophilus) microplus (canestrini), along with the concentration-time blood sera profile in treated cattle. the therapeutic efficacy against ticks of all parasitic stages on cattle at the time of treatment was >99.9%, and the mean tick number, index of fecundity, engorgement weight, and egg mass weight ... | 2011 | 21485367 |
| efficacy of spinosad against acaricide-resistant and -susceptible rhipicephalus (boophilus) microplus and acaricide-susceptible amblyomma americanum and dermacentor variabilis. | various acaricide-resistant strains of rhipicephalus (boophilus) microplus, representative of the major resistance mechanisms found in mexico and brazil, were exposed to spinosad using the food and agriculture organization of the united nations larval packet test and adult immersion test (ait). larvae of all strains tested were found to be susceptible to spinosad. conversely, spinosad did not show toxic activity toward engorged females used in the ait. in vitro tests against larvae, nymphs, and ... | 2011 | 21485374 |
| functional characterization of two defensin isoforms of the hard tick ixodes ricinus. | abstract: | 2011 | 21504572 |
| temporal characterisation of the organ-specific rhipicephalusmicroplus transcriptional response to anaplasma marginale infection. | arthropods transmit important infectious diseases of humans and animals. importantly, replication and the development of pathogen infectivity are tightly linked to vector feeding on the mammalian host; thus analysis of the transcriptomes of both vector and pathogen during feeding is fundamental to understanding transmission. using anaplasma marginale infection of rhipicephalusmicroplus as the experimental model, we tested three hypotheses exploring the temporal and organ-specific nature of the t ... | 2011 | 21514300 |
| copper nanoparticles synthesized by polyol process used to control hematophagous parasites. | the present study was based on assessments of the anti-parasitic activities of the hematophagous (blood feeding) larvae of malaria vector, anopheles subpictus grassi, filariasis vector, culex quinquefasciatus, say (diptera: culicidae), and the larvae of cattle tick rhipicephalus (boophilus) microplus, canestrini (acari: ixodidae). the metallic copper nanoparticles (cu nps) synthesized by polyol process from copper acetate as precursor and tween 80 were used as both the medium and the stabilizing ... | 2011 | 21526405 |
| detection and differentiation of borrelia burgdorferi sensu lato in ticks collected from sheep and cattle in china. | abstract: | 2011 | 21529349 |
| boophilus microplus cathepsin l-like (bmcl1) cysteine protease: specificity study using a peptide phage display library. | the tick rhipicephalus (boophilus) microplus is one of the most important bovine ectoparasites, a disease vector responsible for losses in meat and milk productions. a cysteine protease similar to cathepsin l, named bmcl1, was previously identified in r. microplus gut, suggesting a role of the enzyme in meal digestion. in this work, bmcl1 was successfully expressed in pichia pastoris system, yielding 54.8mg/l of culture and its activity was analyzed by synthetic substrates and against a r. micro ... | 2011 | 21536386 |
| in vitro acaricidal activity of neem (azadirachta indica) seed extracts with known azadirachtin concentrations against rhipicephalus microplus. | the effect of four extracts from neem seeds (azadirachta indica) containing 2000, 5000, 9000 and 10,000ppm of azadirachtin a (aza), quantified by high-performance liquid chromatography (hplc) and diluted to 1.25%; 2.5%; 5.0%; 10.0% and 12.8% was verified by in vitro tests with engorged females and larvae of the cattle tick rhipicephalus microplus. the results from the bioassays with the engorged females showed that the main toxic effect of the extracts was reduction of the reproductive parameter ... | 2011 | 21536387 |
| a calcium bioluminescence assay for functional analysis of mosquito (<em>aedes aegypti</em>) and tick (<em>rhipicephalus microplus</em>) g protein-coupled receptors. | arthropod hormone receptors are potential targets for novel pesticides as they regulate many essential physiological and behavioral processes. the majority of them belong to the superfamily of g protein-coupled receptors (gpcrs). we have focused on characterizing arthropod kinin receptors from the tick and mosquito. arthropod kinins are multifunctional neuropeptides with myotropic, diuretic, and neurotransmitter function. here, a method for systematic analyses of structure-activity relationships ... | 2011 | 21540818 |
| evaluation of the efficacy of acaricides used to control the cattle tick, rhipicephalus microplus, in dairy herds raised in the brazilian southwestern amazon. | the adult immersion test (ait) was used to evaluate the efficacy of acaricide molecules used for control of rhipicephalus microplus on 106 populations collected in five municipalities in the state of rondônia in the brazilian south occidental amazon region. the analysis of the data showed that the acaricide formulations had different efficacies on the tick populations surveyed. the synthetic pyrethroids (sps) acaricides were the least effective (48.35-76.84%), followed by sp + organophosphate (o ... | 2011 | 21547224 |
| evolution of acaricide resistance: phenotypic and genotypic changes in field populations of rhipicephalus (boophilus) microplus in response to pyrethroid selection pressure. | there have been few, if any, studies of arthropod field populations quantifying the kinetics of evolution of phenotypic and genotypic resistance to chemicals in response to the presence or absence of selection pressure. a prospective intervention study was undertaken over 2years in mexico to measure changes in resistance phenotype and genotype in the presence or absence of pyrethroid selection pressure on field populations of rhipicephalus (boophilus) microplus ticks on 11 farms with varying deg ... | 2011 | 21570981 |
| effect of metarhizium anisopliae fungus on off-host rhipicephalus (boophilus) microplus from tick-infested pasture under cattle grazing in brazil. | this work aimed to assess the effect of the fungus metarhizium anisopliae on off-host rhipicephalus (boophilus) microplus from tick-infested brachiaria decumbens pasture undergoing cattle grazing. for this purpose a naturally tick-infested brachiaria decumbens pasture of 60m×100m with twelve grazing holstein friesian-nelore (bos indicus) cross breed bovines was sprayed 12 times, 21 days apart with an aqueous conidial suspension of the e9 isolate of m. anisopliae fungus. control pasture was treat ... | 2011 | 21571437 |
| enhancing the stress tolerance and virulence of an entomopathogen by metabolic engineering dhn-melanin biosynthesis genes. | entomopathogenic fungi have been used for biocontrol of insect pests for many decades. however, the efficacy of such fungi in field trials is often inconsistent, mainly due to environmental stresses, such as ultraviolet radiation, temperature extremes, and desiccation. to circumvent these hurdles, metabolic engineering of dhn-melanin biosynthetic genes (polyketide synthase, scytalone dehydratase, and 1,3,8-trihydroxynaphthalene reductase) cloned from alternaria alternata were transformed into th ... | 2011 | 21571888 |
| effect of water flooding on the oviposition capacity of engorged adult females and hatchability of eggs of dog ticks: rhipicephalus sanguineus and haemaphysalis leachi leachi. | effects of water flooding on the oviposition capacity of engorged adult females and hatchability of eggs of rhipicephalus sanguineus and haemaphysalis leachi leachi under laboratory conditions were investigated. the durations of time of water flooding were 1, 2, 4, 6, 12, 24, 48, 72, 96, and 120 hours. engorged females of r. sanguineus and h. leachi leachi did not oviposit after being flooded for more than 48 and 6 hours, respectively. the preoviposition periods of both species were longer than ... | 2011 | 21584237 |
| a snapshot of the ixodes scapularis degradome. | parasitic encoded proteases are essential to regulating interactions between parasites and their hosts and thus they represent attractive anti-parasitic druggable and/or vaccine target. we have utilized annotations of ixodes scapularis proteases in gene bank and version 9.3 merops database to compile an index of at least 233 putatively active and 150 putatively inactive protease enzymes that are encoded by the i. scapularis genome. the 233 putatively active protease homologs hereafter referred t ... | 2011 | 21596113 |
| diazinon resistant status in rhipicephalus (boophilus) microplus collected from different agro-climatic regions of india. | the resistance status of rhipicephalus (boophilus) microplus to "diazinon" was evaluated in 20 locations situated at various agro-climatic regions of india. adult immersion test (ait) was optimized using laboratory reared acaricide susceptible ivri-i strain of r. (b.) microplus and minimum effective concentration of diazinon was determined as 635.2ppm. the discriminating dose (dd) was worked out as 1270.4ppm and was tested on female ticks collected from organized and unorganized farms located at ... | 2011 | 21600695 |
| tick-borne bacteria in free-living jaguars (panthera onca) in pantanal, brazil. | abstract tick-borne bacteria were investigated in 10 free-living jaguars and their ticks in the pantanal biome, brazil. jaguar sera were tested by indirect fluorescent antibody assays using rickettsia rickettsii, rickettsia parkeri, rickettsia amblyommii, rickettsia rhipicephali, rickettsia felis, rickettsia bellii, ehrlichia canis, and coxiella burnetii as crude antigens. all 10 jaguar sera reacted (titer ≥64) to at least one rickettsia species; 4 and 3 sera reacted with e. canis and c. burneti ... | 2011 | 21612532 |
| rhipicephalus (boophilus) microplus embryo proteins as target for tick vaccine. | rhipicephalus (boophilus) microplus is one of the most widely distributed tick in the world. the control of the parasite is based mainly on the use of chemical acaricides, which are produced from a limited set of molecules. these drugs induce selection of acaricide-resistant ticks, and are an important source of environmental pollution. an approach based on anti-tick vaccines may circumvent these obstacles. characterization of the physiological function of tick molecules may be useful to develop ... | 2011 | 21620488 |
| vaccines to combat the neglected tropical diseases. | the neglected tropical diseases (ntds) represent a group of parasitic and related infectious diseases such as amebiasis, chagas disease, cysticercosis, echinococcosis, hookworm, leishmaniasis, and schistosomiasis. together, these conditions are considered the most common infections in low- and middle-income countries, where they produce a level of global disability and human suffering equivalent to better known conditions such as human immunodeficiency virus/acquired immunodeficiency syndrome an ... | 2011 | 21198676 |
| assessment of bacterial diversity in the cattle tick rhipicephalus (boophilus) microplus through tag-encoded pyrosequencing. | ticks are regarded as the most relevant vectors of disease-causing pathogens in domestic and wild animals. the cattle tick, rhipicephalus (boophilus) microplus, hinders livestock production in tropical and subtropical parts of the world where it is endemic. tick microbiomes remain largely unexplored. the objective of this study was to explore the r. microplus microbiome by applying the bacterial 16s tag-encoded flx-titanium amplicon pyrosequencing (btefap) technique to characterize its bacterial ... | 2011 | 21211038 |
| the penultimate arginine of the carboxyl terminus determines slow desensitization in a p2x receptor from the cattle tick boophilus microplus. | p2x ion channels have been functionally characterized from a range of eukaryotes. although these receptors can be broadly classified into fast and slow desensitizing, the molecular mechanisms underlying current desensitization are not fully understood. here, we describe the characterization of a p2x receptor from the cattle tick boophilus microplus (bmp2x) displaying extremely slow current kinetics, little desensitization during atp application, and marked rundown in current amplitude between se ... | 2011 | 21212138 |
| hemelipoglycoprotein from the ornate sheep tick, dermacentor marginatus: structural and functional characterization. | tick carrier proteins are able to bind, transport, and store host-blood heme, and thus they function also as antioxidants. nevertheless, the role of carrier proteins in ticks is not fully understood. some of them are found also in tick males which do not feed on hosts to such an extent such as females (there are differences in male feeding in different tick species) and thus they are not dealing with such an excess of heme; some of the carrier proteins were found in salivary glands where the pro ... | 2011 | 21214898 |
| determination of the susceptibility of rhipicephalus (boophilus) microplus (acari: ixodidae) to ivermectin and fipronil by larval immersion test (lit) in uruguay. | rhipicephalus (boophilus) microplus is an important cattle pest in uruguay, and the law regulates its control. it is resistant to organophosphates, synthetic pyrethroids and, as recently discovered, to fipronil. resistance to macrocyclic lactones (mls) and amitraz have not been documented; however, veterinarians and farmers have reported treatment failures. the objective of the present work was to study the susceptibility of cattle tick strains from different uruguayan counties to ivermectin (iv ... | 2011 | 21277092 |
| gender-associated genes in filarial nematodes are important for reproduction and potential intervention targets. | a better understanding of reproductive processes in parasitic nematodes may lead to development of new anthelmintics and control strategies for combating disabling and disfiguring neglected tropical diseases such as lymphatic filariasis and onchocerciasis. transcriptomatic analysis has provided important new insights into mechanisms of reproduction and development in other invertebrates. we have performed the first genome-wide analysis of gender-associated (ga) gene expression in a filarial nema ... | 2011 | 21283610 |
| control of rhipicephalus (boophilus) microplus infestations by the combination of subolesin vaccination and tick autocidal control after subolesin gene knockdown in ticks fed on cattle. | tick subolesin was shown in immunization trials using the recombinant protein to protect hosts against tick infestations. in this study, we demonstrated that subolesin vaccination and release of ticks after subolesin knockdown by rna interference (rnai) could be used for the control of rhipicephalus (boophilus) microplus tick infestations in cattle and suggested that the combination of these methods could increase the efficacy of cattle tick control under some circumstances. the greatest tick co ... | 2011 | 21288805 |
| haplotypes of the bovine igg2 heavy gamma chain in tick-resistant and tick-susceptible breeds of cattle. | bovines present contrasting, heritable phenotypes of infestations with the cattle tick, rhipicephalus (boophilus) microplus. tick salivary glands produce igg-binding proteins (igbps) as a mechanism for escaping from host antibodies that these ectoparasites ingest during blood meals. allotypes that occur in the constant region of igg may differ in their capacity to bind with tick igbps; this may be reflected by the distribution of distinct allotypes according to phenotypes of tick infestations. i ... | 2011 | 21301827 |
| rna interference in ticks. | ticks are obligate hematophagous ectoparasites of wild and domestic animals and humans, and are considered to be second worldwide to mosquitoes as vectors of human diseases(1) and the most important vectors affecting cattle industry worldwide(2). ticks are classified in the subclass acari, order parasitiformes, suborder ixodida and are distributed worldwide from arctic to tropical regions(3). despite efforts to control tick infestations, these ectoparasites remain a serious problem for human and ... | 2011 | 21304465 |
| resistance to cypermethrin, deltamethrin and chlorpyriphos in populations of rhipicephalus (boophilus) microplus (acari: ixodidae) from small farms of the state of são paulo, brazil. | a field survey of resistance was conducted based on the larval packet test technique with synthetic pyrethroids (cypermethrin and deltamethrin) and organophosphates (chlorpyriphos) in rhipicephalus (boophilus) microplus field populations from six different regions of the state of são paulo (brazil). 82.6% of the populations showed resistance to cypermethrin, 86.36% to deltamethrin and 65.25% to chlorpyriphos, with 50% presenting resistance to both sp and op acaricide. according to the questionna ... | 2011 | 21306827 |
| oocysts of hepatozoon canis in rhipicephalus (boophilus) microplus collected from a naturally infected dog. | canine hepatozoonosis is a tick-borne disease caused by protozoans of the genus hepatozoon. several tick species have been implicated as potential vectors. therefore, extensive studies are needed to determine the 'natural' endemic cycle of this parasite. this paper presents the first report of the presence of hepatozoon canis oocysts in rhipicephalus (boophilus) microplus collected from an infected dog. | 2011 | 21324597 |
| acaricidal, pediculocidal and larvicidal activity of synthesized zno nanoparticles using wet chemical route against blood feeding parasites. | the present study was based on assessments of the anti-parasitic activities to determine the efficacies of synthesized zinc oxide nanoparticles (zno nps) prepared by wet chemical method using zinc nitrate and sodium hydroxide as precursors and soluble starch as stabilizing agent against the larvae of cattle tick rhipicephalus (boophilus) microplus, canestrini (acari: ixodidae); head louse pediculus humanus capitis, de geer (phthiraptera: pediculidae); larvae of malaria vector, anopheles subpictu ... | 2011 | 21340566 |
| Vaccination with BM86, subolesin and akirin protective antigens for the control of tick infestations in white tailed deer and red deer. | Red deer (Cervus elaphus) and white-tailed deer (Odocoileus virginianus) are hosts for different tick species and tick-borne pathogens and play a role in tick dispersal and maintenance in some regions. These factors stress the importance of controlling tick infestations in deer and several methods such as culling and acaricide treatment have been used. Tick vaccines are a cost-effective alternative for tick control that reduced cattle tick infestations and tick-borne pathogens prevalence while r ... | 2012 | 22079077 |
| tick cell culture isolation and growth of rickettsia raoultii from dutch dermacentor reticulatus ticks. | tick cell lines play an important role in research on ticks and tick-borne pathogenic and symbiotic microorganisms. in an attempt to derive continuous dermacentor reticulatus cell lines, embryo-derived primary cell cultures were set up from eggs laid by field ticks originally collected as unfed adults in the netherlands and maintained for up to 16 months. after several months, it became evident that cells in the primary cultures were infected with a rickettsia-like intracellular organism. supern ... | 2012 | 23140894 |
| rnai in arthropods: insight into the machinery and applications for understanding the pathogen-vector interface. | the availability of genome sequencing data in combination with knowledge of expressed genes via transcriptome and proteome data has greatly advanced our understanding of arthropod vectors of disease. not only have we gained insight into vector biology, but also into their respective vector-pathogen interactions. by combining the strengths of postgenomic databases and reverse genetic approaches such as rnai, the numbers of available drug and vaccine targets, as well as number of transgenes for su ... | 2012 | 24705082 |
| bioinformatics and expression analyses of the ixodes scapularis tick cystatin family. | the cystatins are inhibitors of papain- and legumain-like cysteine proteinases, classified in merops subfamilies i25a-i25c. this study shows that 84 % (42/50) of tick cystatins are putatively extracellular in subfamily i25b and the rest are putatively intracellular in subfamily i25a. on the neighbor joining phylogeny guide tree, subfamily i25a members cluster together, while subfamily i25b cystatins segregate among prostriata or metastriata ticks. two ixodes scapularis cystatins, aay66864 and is ... | 2012 | 23053911 |
| analysis of babesia bovis infection-induced gene expression changes in larvae from the cattle tick, rhipicephalus (boophilus) microplus. | cattle babesiosis is a tick-borne disease of cattle that has severe economic impact on cattle producers throughout the world's tropical and subtropical countries. the most severe form of the disease is caused by the apicomplexan, babesia bovis, and transmitted to cattle through the bite of infected cattle ticks of the genus rhipicephalus, with the most prevalent species being rhipicephalus (boophilus) microplus. we studied the reaction of the r. microplus larval transcriptome in response to infe ... | 2012 | 22871314 |
| oral vaccination with vaccinia virus expressing the tick antigen subolesin inhibits tick feeding and transmission of borrelia burgdorferi. | immunization with the ixodes scapularis protein, subolesin, has previously been shown to protect hosts against tick infestation and to decrease acquisition of anaplsma marginale and babesia bigemina. here we report the efficacy of subolesin, a conserved tick protein that can act as a regulator of gene expression, expressed from vaccinia virus for use as an orally delivered reservoir - targeted vaccine for prevention of tick infestation and acquisition/transmission of borrelia burgdorferi to its ... | 2012 | 22864146 |
| gene silencing in tick cell lines using small interfering or long double-stranded rna. | gene silencing by rna interference (rnai) is an important research tool in many areas of biology. to effectively harness the power of this technique in order to explore tick functional genomics and tick-microorganism interactions, optimised parameters for rnai-mediated gene silencing in tick cells need to be established. ten cell lines from four economically important ixodid tick genera (amblyomma, hyalomma, ixodes and rhipicephalus including the sub-species boophilus) were used to examine key p ... | 2012 | 22773071 |
| tick salivary secretion as a source of antihemostatics. | ticks are mostly obligatory blood feeding ectoparasites that have an impact on human and animal health. in addition to direct damage due to feeding, some tick species serve as the vectors for the causative agents of several diseases, such as the spirochetes of the genus borrelia causing lyme disease, the virus of tick-borne encephalitis, various rickettsial pathogens or even protozoan parasites like babesia spp. hard ticks are unique among bloodfeeders because of their prolonged feeding period t ... | 2012 | 22564820 |
| the role of cystatins in tick physiology and blood feeding. | ticks, as obligate hematophagous ectoparasites, impact greatly on animal and human health because they transmit various pathogens worldwide. over the last decade, several cystatins from different hard and soft ticks were identified and biochemically analyzed for their role in the physiology and blood feeding lifestyle of ticks. all these cystatins are potent inhibitors of papain-like cysteine proteases, but not of legumain. tick cystatins were either detected in the salivary glands and/or the mi ... | 2012 | 22647711 |
| detection of wolbachia in the tick ixodes ricinus is due to the presence of the hymenoptera endoparasitoid ixodiphagus hookeri. | the identification of micro-organisms carried by ticks is an important issue for human and animal health. in addition to their role as pathogen vectors, ticks are also the hosts for symbiotic bacteria whose impact on tick biology is poorly known. among these, the bacterium wolbachia pipientis has already been reported associated with ixodes ricinus and other tick species. however, the origins of wolbachia in ticks and their consequences on tick biology (known to be very diverse in invertebrates, ... | 2012 | 22292021 |
| ticks on buffaloes (bubalus bubalis) in the state of rio de janeiro, brazil. | some tick species have been described parasitizing buffaloes, but reports from brazil are rare. this study aimed to ascertain the species of ticks that parasitize buffaloes in the state of rio de janeiro. seventeen farm properties were visited between june 2008 and december 2009, and buffaloes were inspected in relation to infestation. the ticks were identified and a semi-structured questionnaire on parasitism and treatment against ticks was filled out through interviews with the owners. the spe ... | 2012 | 23070448 |
| ivermectin resistance status and factors associated in rhipicephalus microplus (acari: ixodidae) populations from veracruz, mexico. | the objectives of the present study were to (1) determine the status of resistance or susceptibility to ivermectin (ivm) in rhipicephalus microplus tick populations in veracruz, mexico, (2) determine the level of resistance (resistance ratios [rr] and lethal concentrations for 50% [lc(50)] and 99% [lc(99)]) mortality in each r. microplus population, and (3) identify factors associated with resistance. populations of r. microplus were sampled from 53 cattle farms to evaluate their resistance usin ... | 2012 | 22785128 |
| chemical composition and acaricidal activity of essential oil from lippia sidoides on larvae of dermacentor nitens (acari: ixodidae) and larvae and engorged females of rhipicephalus microplus (acari: ixodidae). | the aim of this work was to identify the compounds and to investigate the acaricidal activity of the essential oil from the leaves of lippia sidoides on rhipicephalus microplus and dermacentor nitens. the oil was obtained by hydrodistillation and analyzed by gas chromatography (gc/fid) and gas chromatography/mass spectrometry. in total, 15 compounds comprising 99.97 % of the total peak area were identified. the main constituent of the essential oil was thymol (67.60 %). the acaricidal activity w ... | 2012 | 22983219 |
| acaricidal activity of eugenol on rhipicephalus microplus (acari: ixodidae) and dermacentor nitens (acari: ixodidae) larvae. | the aim of this study was to evaluate the acaricidal activity of eugenol, with different solubilizations and concentrations, on rhipicephalus microplus and dermacentor nitens larvae and to determine the lethal time. the study consisted of four experiments, and the mortality was assessed using the larval packet test with adaptations. the mortality observed in the first experiment was 100 % for all the groups treated with eugenol solubilized in different solvents. in the second, the hydroethanolic ... | 2012 | 22622689 |
| abc transporters as a multidrug detoxification mechanism in rhipicephalus (boophilus) microplus. | atp-binding cassette (abc) transporters are responsible for pumping drugs across membranes and are an important drug detoxification mechanism. since abc transporters act on a wide spectrum of chemical compounds, they have been associated with multidrug resistance phenotype in various parasites and cancer cells. here, we document the presence of a rhipicephalus (boophilus) microplus tick population (jaguar) resistant to four acaricide classes (organophosphates (op), synthetic pyrethroids (sp), am ... | 2012 | 22926678 |
| bovine immunoprotection against rhipicephalus (boophilus) microplus with recombinant bm86-campo grande antigen. | the southern cattle fever tick, rhipicephalus (boophilus) microplus, is no doubt the most economically important ectoparasite of cattle globally. the inappropriate use of chemical acaricides has driven the evolution of resistance in populations of r. (b.) microplus. anti-tick vaccines represent a technology that can be combined with acaricides in integrated control programs to mitigate the impact of r. (b.) microplus. the recombinant form of bm86 antigen from the campo grande (rbm86-cg) strain o ... | 2012 | 23070436 |
| principal component analysis on the perceptions of milk producers about rhipicephalus (boophilus) microplus control in minas gerais. | milk producers in lavras, passos and divinópolis, minas gerais, were interviewed with the aim of evaluating their perceptions and attitudes regarding control over rhipicephalus (boophilus) microplus. multivariate correlation between the variables was done by means of principal component analysis. the producers' perceptions and attitudes regarding r. (b.) microplus were similar: most of them did not have any basic knowledge of tick biology or control, and they applied acaricide products through b ... | 2012 | 23070431 |
| efficacy of hyalomma scupense (hd86) antigen against hyalomma excavatum and h. scupense tick infestations in cattle. | the rhipicephalus microplus recombinant bm86-based tick vaccines have shown their efficacy for the control of several hyalomma cattle ticks genera, namely h. dromedarii and h. anatolicum. however, h. scupense species, the most important tick in north africa has never been studied. vaccination trials using either a recombinant bm86-based vaccine or a recombinant hd86-based vaccine (the bm86 ortholog in h. scupense) were conducted in cattle against immature and adult h. scupense ticks and adult h. ... | 2012 | 23036501 |
| acaricidal action of destruxins produced by a marine-derived beauveria felina on the bovine tick rhipicephalus (boophilus) microplus. | the increasing resistance of rhipicephalus (boophilus) microplus tick to commercial insecticides requires alternative methods for the control of this cattle plague. the enthomopathogenic fungus beauveria felina produces destruxins in culture media, cyclic depsipeptides which display an array of biological activities. the present investigation aimed to evaluate the acaricide action of destruxins isolated from b. felina culture media on r. (b.) microplus engorged females. b. felina was grown in mf ... | 2012 | 22955115 |
| [survey on ticks and detection of new bunyavirus in some vect in the endemic areas of fever, thrombocytopenia and leukopenia syndrome (ftls) in henan province]. | to investigate the distribution, species, seasonal fluctuation of ticks and detect new bunyavirus in some hematophagus in the endemic areas of fever thrombocytopenia and leukopenia syndrome (ftls) in henan province. | 2012 | 22943894 |
| exploring the use of an anti-tick vaccine as a tool for the integrated eradication of the cattle fever tick, rhipicephalus (boophilus) annulatus. | bovine babesiosis, also known as cattle fever, is a tick-borne protozoal disease foreign to the united states. it was eradicated by eliminating the vector species, rhipicephalus (boophilus) annulatus and rhipicephalus (boophilus) microplus, through the efforts of the cattle fever tick eradication program (cftep), with the exception of a permanent quarantine zone (pqz) in south texas along the border with mexico. keeping the u.s. free of cattle fever ticks in a sustainable manner is a critical na ... | 2012 | 22687762 |
| esterase profile of rhipicephalus (boophilus) microplus populations collected from northern india exhibiting varied susceptibility to deltamethrin. | rhipicephalus (boophilus) microplus is an economically important ectoparasite of cattle. chemical acaricides remain the most practical method for control of these pests. during past two decades there have been increasing reports of resistance development against synthetic pyrethroids in tick populations of this species throughout the world. a study was conducted to determine the level of susceptibility of r. (b.) microplus to deltamethrin collected from different geographical locations of northe ... | 2012 | 22669281 |
| phylogenetic analysis of benign theileria species based on major piroplasm surface protein (mpsp) genes from ticks of grazing cattle in korea. | complete major piroplasm surface protein (mpsp) gene sequences of benign theileria parasites were isolated from ticks of grazing cattle in korea. a total of 556 tick samples were collected in five provinces: chungbuk, jeonbuk, jeonnam, gyeongbuk, and jeju during 2010-2011. fifteen samples from chungbuk and jeonnam were positive for the theileria mpsp gene by pcr amplification using a specific primer set. a phylogenetic tree was constructed with the amplified gene sequences and 26 additional sequ ... | 2012 | 22647464 |
| association between ecological factors and the presence of rhipicephalus (boophilus) microplus larvae in puerto rico. | a prevalence study was conducted to survey tick larvae populations in puerto rico (pr), compare the number of infested sites with rhipicephalus (boophilus) microplus larvae between the wet and dry season, and assess the associations of ecologic factors on the presence of r. microplus larvae. ninety-six sites were selected using a gis-based sampling method. each site was sampled twice; the first sampling was performed during the dry season (march 4-18, 2007) and the second sampling during the wet ... | 2012 | 22638941 |
| differential recognition by tick-resistant cattle of the recombinantly expressed rhipicephalus microplus serine protease inhibitor-3 (rms-3). | rhipicephalus microplus is an important bovine ectoparasite, widely distributed in tropical and subtropical regions of the world causing large economic losses to the cattle industry. its success as an ectoparasite is associated with its capacity to disarm the antihemostatic and anti-inflammatory reactions of the host. serpins are protease inhibitors with an important role in the modulation of host-parasite interactions. the cdna that encodes for a r. microplus serpin was isolated by race and sub ... | 2012 | 22608113 |
| deltamethrin and cypermethrin resistance status of rhipicephalus (boophilus) microplus collected from six agro-climatic regions of india. | a cross sectional study was conducted to assess the prevalence of synthetic pyrethroids (sp) resistance in rhipicephalus (boophilus) microplus in india. twenty-seven areas located in six agro-climatic regions were selected for the collection of engorged ticks using two stage stratified sampling procedure. adult immersion test (ait) and larval packet test (lpt) were optimized using laboratory reared susceptible line of r.(b.) microplus (ivri-i) for determination of 95% lethal concentration (lc(95 ... | 2012 | 22541585 |
| resistance of rhipicephalus microplus to amitraz and cypermethrin in tropical cattle farms in veracruz, mexico. | the objectives of this study were: (1) to determine the prevalence and factors associated with rhipicephalus microplus , resistant to cypermethrin and amitraz, from cattle farms in veracruz, mexico, and (2) to determine in vitro mortality percentages of field populations of r. microplus exposed to discriminating doses (dd) of cypermethrin and amitraz. fifty-three populations of r. microplus were tested by bioassays using dd of cypermethrin (0.05%) and amitraz (0.0002%). the prevalence of cattle ... | 2012 | 22524292 |
| expression and functional characterization of boophilin, a thrombin inhibitor from rhipicephalus (boophilus) microplus midgut. | rhipicephalus (boophilus) microplus is an ectoparasite responsible for an important decrease in meat, milk and leather production, caused both by cattle blood loss and by the transmission of anaplasmosis and babesiosis. r. microplus is a rich source of serine protease inhibitors, including the trypsin inhibitors bmti-a and bmti-6, the subtilisin inhibitor bmsi, and the recently described thrombin inhibitor, boophilin. boophilin is a double kunitz-type thrombin inhibitor, with the unusual ability ... | 2012 | 22341830 |
| new foci of rhipicephalus microplus in west africa. | the invasive character of rhipicephalus microplus was observed in benin, the second west-african country from which this ticks species has been collected after the initial confirmed record in ivory coast in 2007. a cross-sectional study was carried out in the department of mono to examine the presence of the tick r. microplus. the survey covered 9 herds (villages) in an agro-ecological zone inhabited by agro-pastoralists, including the state farm of kpinnou that imported girolando cattle from br ... | 2012 | 22286115 |
| nomuraea rileyi as biological control agents of rhipicephalus microplus tick. | nomuraea rileyi, a fungus pathogenic to insects, has been widely used for biological control of agricultural pests in brazil. this study investigates the effects of n. rileyi, isolates nr 138, nr 151, and nr 177, to eggs, larvae, and engorged females of rhipicephalus microplus tick. specimens were immersed in 1 ml of conidial suspension for 3 min, whereas the control group was immersed in 0.01% tween 80 water solution. the isolate nr 138 controlled 67.37% of ticks when the highest conidial conce ... | 2012 | 22782472 |
| multi-antigenic vaccine against the cattle tick rhipicephalus (boophilus) microplus: a field evaluation. | the tick rhipicephalus (boophilus) microplus is a blood-sucking ectoparasite of cattle that severely impairs livestock production. studies on tick immunological control address mostly single-antigen vaccines. however, from the commercial standpoint, so far no single-antigen vaccine has afforded appropriate protection against all r. microplus populations. in this context, multi-antigen cocktails have emerged as a way to enhance vaccine efficacy. in this work, a multi-antigenic vaccine against r. ... | 2012 | 22981764 |
| comparative efficacy of rhaa86 and rbm86 against hyalomma anatolicum anatolicum and rhipicephalus (boophilus) microplus. | hyalomma anatolicum anatolicum and rhipicephalus (boophilus) microplus are the most economically important tick species in india and other tropical and subtropical regions of the world and transmit pathogens causing animal and human diseases. we demonstrated that vaccination of animal by rhaa86 could be used for the control of both h. a. anatolicum and r. (b.) microplus infestations. by comparing the efficacy of rhaa86 and rbm86, it was observed that vaccine based on rhaa86 will be more effectiv ... | 2012 | 22313386 |
| molecular characterization of bm86 gene orthologs from hyalomma excavatum, hyalomma dromedarii and hyalomma marginatum marginatum and comparison with a vaccine candidate from hyalomma scupense. | the ixodid ticks from the hyalomma genus are important pests of livestock, having major medical and veterinary significance in northern africa. beside their direct pathogenic effects, these species are vectors of important diseases of livestock and in some instances of zoonoses. anti-tick vaccines developed in australia and cuba based on the concealed antigen bm86 have variable efficacy against h. anatolicum and h. dromedarii. this variation in vaccine efficacy could be explained by the variabil ... | 2012 | 22683299 |
| geographic distribution of the invasive cattle tick rhipicephalus microplus, a country-wide survey in benin. | the cattle tick rhipicephalus microplus is currently invading the west african region, and little information is available on the spread of this exotic tick in this region. we set out a country-wide field survey to determine its current distribution in benin. ticks were collected on cattle from 106 farms selected by random sampling covering all regions of the country. rhipicephalus annulatus was found on 70 % of all farms, r. decoloratus on 42 %, r. geigyi on 58 %, and r. microplus on 49 %. ther ... | 2012 | 22689006 |
| bioengineered surfaces to improve the blood compatibility of biomaterials through direct thrombin inactivation. | thrombus formation, due to thrombin generation, is a major problem affecting blood-contacting medical devices. this work aimed to develop a new strategy to improve the hemocompatibility of such devices by the immobilization of a naturally occurring thrombin inhibitor into a nanostructured surface. boophilin, a direct thrombin inhibitor from the cattle tick rhipicephalus microplus, was produced as a recombinant protein in pichia pastoris. boophilin was biotinylated and immobilized on biotin-termi ... | 2012 | 22846590 |
| spectroscopic evaluation of thymol dissolved by different methods and influence on acaricidal activity against larvae of rhipicephalus microplus (acari: ixodidae). | the acaricidal activity of three thymol formulations was investigated at five concentrations (1.25, 2.5, 5.0, 7.5, and 10.0 mg/ml) on rhipicephalus microplus larvae, and the behavior of its solubility in these formulations was analyzed. the thymol was dissolved in distilled water plus 1 % dimethylsulfoxide as adjuvant under two heating regimes (water bath in formulation 1 and hot plate in formulation 2) as well as without heating in 50 % ethanol and 50 % water (v/v). the acaricidal activity was ... | 2012 | 22797607 |
| the quest for a universal vaccine against ticks: cross-immunity insights. | as blood-sucking parasites, ticks inflict great damage to animals and humans in many parts of the world. the continued use of chemical acaricides is not sustainable due to increasing tick resistance, growing public concern over drug residues in food and in the environment, and the high cost of developing new acaricides. therefore, an alternative control strategy is urgently needed. vaccines against ticks have been shown to be functionally feasible, as highlighted by the success of bm86 vaccines ... | 2012 | 22766309 |