Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| measurement of adenine uptake as an in vitro screen for antitrichomonal agents. | a semi-automated microdilution test was adapted for the in vitro testing of potential antitrichomonal agents. washed cell suspensions of the pathogenic protozoan trichomonas vaginalis were incubated in microtitration plates for 4 h at 37 degrees c in the presence radiolabelled adenine and serial dilutions of the test compounds. after automatic harvesting of the cells onto filter paper discs, the extent of adenine uptake was estimated by scintillation counting. from the dose-response data it was ... | 1985 | 3872780 |
| isolation and characterization of dna from tritrichomonas foetus and trichomonas vaginalis. | high molecular weight dna samples free of contaminating proteins or rna were obtained from tritrichomonas foetus or trichomonas vaginalis by lysing the cells in 4 m guanidinium thiocyanate before centrifuging in cscl density gradient and then purifying the dna band by nacs-37 column chromatography. the bulk dna from either organism acted as a single component in ion-exchange chromatography, agarose gel electrophoresis, cscl density gradient centrifugation and thermal denaturation. t. foetus dna ... | 1985 | 3873006 |
| [trichomoniasis of the urogenital tract in patients hospitalized in 1976-1983]. | we report on investigations carried out on t. vaginalis and mixed infection (t. vaginalis and yeast-like fungi). special attention was paid to metronidazol resistance on t. vaginalis strains isolated from patients who had been repeatedly treated with this medicament. therefore we point to the necessity of defining the patients' susceptibility to this remedy. | 1985 | 3873142 |
| use of a time-kill technique for susceptibility testing of trichomonas vaginalis. | the emergence of metronidazole-resistant trichomonas vaginalis and questions about the safety of metronidazole are significant concerns in treatment of trichomoniasis. at 24 h, a microtiter assay was used to test antimicrobial susceptibility of 16 recent isolates; the mics of metronidazole ranged from less than 0.06 to 25 micrograms/ml. observable motility as an endpoint correlated imperfectly with survival as measured in pour plates. quantitative pour plate cultures of six t. vaginalis isolates ... | 1985 | 3873214 |
| prevalence of low genital tract infectious agents in young israeli women. | low genital tract infectious agents were examined 411 times in 386 young women. each examination included anamnesis, physical examination and broad-spectrum microbiologic analysis for the detection of: ureaplasma urealyticum, chlamydia trachomatis, trichomonas vaginalis, candida species, mycoplasma hominis, streptococcus beta hemolyticus group b, hemophilus vaginalis, neisseria gonorrhoeae and syphilis serology. three hundred and six patients were symptomatic for low genital tract infection and ... | 1985 | 3873446 |
| proliferative response of human lymphocytes to secretory and cellular antigens of trichomonas vaginalis. | lymphocytes from patients with active trichomoniasis showed a proliferative response when incubated in the presence of secretory and cellular products of either pathogenic or non-pathogenic trichomonas vaginalis. maximal responses were detected using 50 micrograms per ml protein after 5 days incubation. lymphocytes from non-infected laboratory workers showed no response to these antigens. this indicates that delayed hypersensitivity reactions may act to modulate inflammatory responses in trichom ... | 1985 | 3874274 |
| ultrastructural changes of trichomonas vaginalis prior and after cryopreservation. | fine structural changes of trichomonas vaginalis are described prior and after the freezing process in liquid nitrogen. dimethyl sulfoxide (dmso) used as the cryoprotectant caused distinct alterations of the cytoplasm when trichomonads were equilibrated with 5% dmso under various experimental conditions. changes were bubble-like protrusions, fissuration and/or vacuolation of the cytoplasm, doubling and removal or/and rupture of the cell membrane. apart from these findings cryopreservation caused ... | 1985 | 3874499 |
| trichomonas vaginalis infestation in sexually abused girls. | we report infection with trichomonas vaginalis in four sexually abused girls; two of these patients are unique in that they were premenarchal. we discuss the clinical features in premenarchal and postmenarchal children and relate infection to the likelihood of prior sexual abuse. | 1985 | 3875279 |
| importance of differential diagnosis in acute vaginitis. | acute vaginitis is one of the most common diseases seen in the practice of office gynecology. large survey studies of women with lower genital tract symptoms suggestive of vaginitis have demonstrated the presence of three major etiologic categories in acute vaginitis: (1) nonspecific vaginosis (gardnerella vaginalis), (2) vulvovaginal candidiasis (candida albicans), and (3) trichomoniasis (trichomonas vaginalis). effective treatment of acute vaginitis requires that an accurate diagnosis be estab ... | 1985 | 3875290 |
| 13c-nmr reveals glycerol as an unexpected major metabolite of the protozoan parasite trichomonas vaginalis. | 13c-nmr has been used to study the kinetics of the formation of metabolites from [1-13c]glucose in intact cells of trichomonas vaginalis during anaerobic incubation. as well as the expected metabolites lactate and acetate, this technique revealed glycerol as an additional major product, present in amounts equimolar with acetate. the formation of glycerol is readily explained in terms of the need to maintain redox balance. this protozoan now joins the small group of organisms which are known to p ... | 1985 | 3876951 |
| vitamin b12 requirement for the growth of trichomonas vaginalis in vitro. | 1985 | 3877156 | |
| specific nature of trichomonas vaginalis parasitism of host cell surfaces. | the adherence of trichomonas vaginalis nyh 286 to host cells was evaluated by using monolayer cultures of hela and hep-2 epithelial cells and human fibroblast cell lines. saturation of sites on hela cells was achieved, yielding a maximal t. vaginalis nyh 286-to-cell ratio of two. the ability of radiolabeled nyh 286 to compete with unlabeled trichomonads for attachment and the time, temperature, and ph-dependent nature of host cell parasitism reinforced the idea of specific parasite-cell associat ... | 1985 | 3877690 |
| contact-dependent cytopathogenic mechanisms of trichomonas vaginalis. | the cytopathogenic mechanisms of trichomonas vaginalis have been debated since the 1940s. we examined the following three proposed pathogenic mechanisms: contact-dependent extracellular killing, cytophagocytosis, and extracellular cytotoxins. serial observations of chinese hamster ovary (cho) cell monolayers exposed to trichomonads revealed that (i) trichomonads form clumps, (ii) the clumps adhere to cells in culture, and (iii) monolayer destruction occurs only in areas of contact with t. vagina ... | 1985 | 3877693 |
| microbiology of the lower genital tract in postmenarchal adolescent girls: differences by sexual activity, contraception, and presence of nonspecific vaginitis. | the prevalence of selected microorganisms in the lower genital tract in postmenarchal adolescent girls was assessed, including vaginal gardnerella vaginalis, group b streptococcus, lactobacillus, mycoplasma species, ureaplasma urealyticum, staphylococcus aureus, and yeast, and endocervical mycoplasma species, u. urealyticum, chlamydia trachomatis, neisseria gonorrhoeae, and trichomonas vaginalis. specific attention was focused on important sexually transmitted disease organisms, and differences ... | 1985 | 3877803 |
| [specific antibodies in women treated for trichomonas vaginitis]. | 1985 | 3878629 | |
| [methods of obtaining a pure culture of trichomonas vaginalis]. | 1985 | 3879061 | |
| [vaginal microbial flora in a normal term pregnancy]. | 1985 | 3879109 | |
| aspartate: 2-oxoglutarate aminotransferase from trichomonas vaginalis. identity of aspartate aminotransferase and aromatic amino acid aminotransferase. | aspartate: 2-oxoglutarate aminotransferase from the anaerobic protozoon trichomonas vaginalis was purified to homogeneity and characterized. it is a dimeric protein of overall mr approx. 100000. only a single isoenzyme was found in t. vaginalis. the overall molecular and catalytic properties have features in common with both the vertebrate cytoplasmic and mitochondrial isoenzymes. the purified aspartate aminotransferase from t. vaginalis showed very high rates of activity with aromatic amino aci ... | 1985 | 3879173 |
| isolation of gardnerella vaginalis from high vaginal swabs. | vaginal swabs from 2715 consecutive unselected women with vaginal discharge were examined for gardnerella vaginalis, candida albicans and trichomonas vaginalis. of 2715 women, 2043 had non-specific vaginitis and g. vaginalis was isolated from 269 cases (13.2%). thirty five of the strains were isolated together with c. albicans, eight with t. vaginalis, six with bacteroides species and three each with group b streptococcus and staphylococcus aureus. two hundred and sixteen (80%) of the isolates w ... | 1985 | 3879398 |
| [ultrastructural study of the in vitro mode of action of p-chlorophenyl bis-(2-hydroxynaphthyl)methane on trichomonas vaginalis]. | 1985 | 3879716 | |
| [antimicrobial activity of nifuratel]. | 1985 | 3879754 | |
| [antimicrobial activity of a douche containing ketoconazole]. | 1985 | 3879755 | |
| [effect of some steroid hormones on the growth of trichomonas vaginalis]. | studies were conducted to determine the effect of some steroid hormones, particularly sex hormones, on the growth of trichomonas vaginalis "in vitro". 17 alpha-estradiol and 17 beta-estradiol were stimulatory for parasite growth, progesterone and testosterone were inhibiting, whereas cholesterol and ergosterol lacked such effects. changes on the growth of t. vaginalis were observed in the first 20 hours of incubation with specific hormonal concentrations. these observations confirm the influence ... | 1985 | 3879810 |
| [detection of specific antibodies against trichomonas vaginalis in human serum]. | 1985 | 3882195 | |
| the treatment of trichomonas vaginalis vaginitis. an open controlled prospective study comparing a single dose of metronidazole tablets, benzoyl metronidazole suspension and tinidazole tablets. | metronidazole tablets, benzoyl metronidazole suspension and tinidazole tablets each given as a single 2 g bolus dose in an open controlled prospective study were found to be effective forms of treatment for trichomonas vaginalis vaginitis. the cure rates on wet smear preparations were 100%, 100% and 94,8% respectively, and symptomatic and observed improvements were excellent. there was a statistically significant difference between the response rates associated with both metronidazole preparatio ... | 1985 | 3885425 |
| detection and characterization of serum antitrichomonal antibodies in urogenital trichomoniasis. | antibodies against trichomonas vaginalis were detected in serum samples from 98 patients by three immunological assays. a good correlation was observed between the enzyme-linked immunosorbent assay and the immunofluorescence method, whereas it was found that the enzyme-linked immunosorbent assay correlated better with the current or past detection of organisms than did other serological methods (immunofluorescence and hemagglutination). similar results were obtained with whole trichomonads and t ... | 1985 | 3886694 |
| [the trichomonads]. | 1985 | 3887632 | |
| [transmission of urogenital trichomoniasis in female prostitutes]. | a group of female prostitutes (n = 41) was found to be infected with trichomonas vaginalis to 36.6%, diagnosed by using culture methods. the examination of these women for t. vaginalis and their treatment is not obligatory, therefore the transmission of these parasites by infected prostitutes is not properly controlled; transmission of the trichomoniasis continues under nearly optimal conditions. it is urgently necessary to examine female prostitutes for t. vaginalis infections without exception ... | 1985 | 3888822 |
| [clinico-experimental study of local humoral immunity of the vagina in human cases of trichomoniasis]. | 1985 | 3890111 | |
| the modes of action of some anti-protozoal drugs. | 1985 | 3892438 | |
| identification of a surface antigen of trichomonas vaginalis. | a major surface antigen of trichomonas vaginalis was purified by using three independently derived monoclonal antibodies (two immunoglobulin m and one immunoglobulin g1) prepared against t. vaginalis phs-2j. a 115,000-molecular-weight antigen and one or more components with a molecular weight of 58,000 to 64,000 were recovered when any of the three antibodies was used as an immunoadsorbent. the purified antigen reacted with all three monoclonal antibodies in an enzyme-linked immunosorbent assay, ... | 1985 | 3894230 |
| problems in the cultivation of cervical bacteria. | multiple variations in the types, species and prevalence of cervical bacteria are described in the literature. a review of the literature was undertaken to determine the effect of delay on the ability to identify bacteria. experiments were initiated to elaborate on those observations. the rate of organism isolation was related to the delay in transport to the laboratory. | 1985 | 3894658 |
| problems in specimen collection for sexually transmitted diseases. | laboratory methods for the diagnosis of sexually transmitted diseases (stds) are continuously undergoing improvement. it remains the responsibility of the clinician to become familiar with the tests available for the diagnosis of stds. those tests depend on obtaining clinical specimens from the proper site and on transporting them to the laboratory under satisfactory conditions. | 1985 | 3894659 |
| relationship of vaginitis to the sex of conceptuses. | the relative viability of x-bearing and y-bearing spermatozoa is influenced by factors in the vagina such as ph. the vaginal environment, in turn, is influenced by its flora. this study examined the relationship of the vaginal flora to the sex of conceptuses. it was found that women who carried trichomonas vaginalis or bacteroides sp or who had nonspecific vaginitis at first prenatal visit were significantly more likely to deliver females than women who carried none of these organisms (54 versus ... | 1985 | 3895073 |
| electron microscope observations on the interaction of mycoplasma fermentans with trichomonas vaginalis. | cultures of trichomonas vaginalis were found to be contaminated with mycoplasma fermentans. by means of electron microscopy the interaction between the prokaryotic organisms and the trichomonads was examined. cells of m. fermentans were observed in the medium; some of them were attached to the surface of the trichomonads and others were observed in membrane-bounded vacuoles of trichomonads. they were also present in the ground substance of the cytoplasm. the mycoplasmas divided by binary fission ... | 1985 | 3895766 |
| [trichomoniasis]. | 1985 | 3902591 | |
| treatment of the gardnerella vaginalis syndrome. a controlled, double-blind study comparing pivampicillin and metronidazole. | a double-blind, randomized, therapeutic study was carried out in 289 patients with vaginal discharge and growth of gardnerella vaginalis (gv) and no growth of neisseria gonorrhoeae, trichomonas vaginalis or candida. treatment consisted of either pivampicillin (pondocillin), 700 mg twice daily for seven days, or metronidazole, 500 mg twice daily for seven days. the effect was evaluated on the basis of the patient's statement and on the result of culture for gv immediately after end of treatment. ... | 1985 | 3903906 |
| [detection of gardnerella vaginalis in the pathogen spectrum of sexually transmissible diseases in vulvovaginitis]. | gardnerella vaginalis, a sexually transmittable organism, is regarded as the indicator of the so-called "non-specific vaginitis". the isolation rate of g. vaginalis from 72 women attending our out-patient department during one year because of urogenital complaints was higher in pretreated than in untreated patients. in genital swabs taken from untreated patients, g. vaginalis could be isolated only in 17%, whereas this was observed in 38% of the specimens from women pretreated with various antim ... | 1985 | 3907172 |
| [analysis of growth curves of trichomonas vaginalis in the presence of staphylococcus aureus and escherichia coli]. | 1985 | 3909648 | |
| the relation between vaginal ph and the microbiological status in vaginitis. | the vaginal ph, microbial flora and presence of clue cells were investigated in 89 women who were seen at a sexually transmitted diseases clinic with a vaginal discharge or because they were contacts of men with gonococcal or non-gonococcal urethritis or because they were seeking a routine examination. none of the women had received antibiotics for at least 4 weeks before examination. a clinically normal vaginal secretion was found in 21 (55%) of 38 women who had a vaginal ph of 5.0-5.5, while s ... | 1985 | 3910080 |
| [vaginal microscopic evidence in women with symptoms of genital infection]. | 1985 | 3915347 | |
| metronidazole inhibition of hydrogen production in vivo in drug-sensitive and resistant strains of trichomonas vaginalis. | h2 production by the human protozoan parasite trichomonas vaginalis was monitored continuously under a mobile gas phase using a membrane-inlet mass spectrometer. simultaneous and continuous measurement of dissolved h2, o2 and co2 indicated that h2 evolution was inhibited at levels of o2 (less than 0.25 microm) undetectable by the technique, whereas co2 production was stimulated. respiration was not stimulated by admitting h2 to the gas phase. metronidazole inhibited both h2 and co2 production. v ... | 1985 | 3921658 |
| further studies on the surface saccharides in trichomonas vaginalis strains by fluorescein-conjugated lectins. | fluorescence emitted by individual cells of several trichomonas vaginalis strains, nearly all of which were cloned, incubated with fluorescein-conjugated lectins in the absence (experimental) or presence (control) of inhibitory sugars, or else in phosphate-buffered saline alone (autofluorescence) was measured with a leitz mpv compact microfluorometer. irrespective of whether the organisms were postfixed in formalin or glutaraldehyde, the relative fluorescence emitted by the cells was closely com ... | 1985 | 3927600 |
| [gas chromatographic-mass spectrometric analysis of gases produced by trichomonas vaginalis in vitro]. | five strains of trichomonas vaginalis were independently inoculated into cysteine-peptone-liver infusion-maltose medium (cplm medium), and gases in the culture bottle were qualitatively analysed at five days after the inoculation by gas chromatography mass spectrometry (gc-ms). methane, ethane and carbon dioxide were commonly found in the gases produced during the culture of trichomonas vaginalis in vitro. carbon monoxide was also found in the gases from 4 of 5 strains. small amount of both ethy ... | 1985 | 3928776 |
| sexually transmitted diseases in females in a juvenile detention center. | of 37 female adolescent prostitutes and 63 other females held in the king county, washington, juvenile detention facility, 81 (81%) complained of vaginal discharge, and only eight (8%) had no genitourinary symptoms. the prevalence of genital infections among prostitutes and nonprostitutes was similar. neisseria gonorrhoeae was found in 18 (18%) of 98 detainees tested, and chlamydia trachomatis was found in 17 (20%) of 86 detainees tested; either organism was found in 27 (32%) of 85 tested for bo ... | 1985 | 3929406 |
| s-adenosylhomocysteine hydrolase activity in trichomonas vaginalis and other trichomonads. | s-adenosylhomocysteine hydrolase has been detected in crude homogenates of trichomonas vaginalis, tritrichomonas foetus and trichomitus batrachorum at activities of 14, 1.2 and 3.3 nmol min-1 mg-1 protein, respectively. the enzyme from t. vaginalis was found to be soluble with ph optimum of 8.0 and apparent km values for adenosine and homocysteine of 100 and 155 microm, respectively. ara a was shown to inhibit the t. vaginalis enzyme but only at relatively high concentration (i50 100 microm), wh ... | 1985 | 3932851 |
| [prevalence of 7 microorganisms in abnormal vaginal secretions (vaginitis)]. | seven microorganisms (n. gonorrhoeae, c. albicans, t. vaginalis, g. vaginalis, m. hominis, u. urealyticum and streptococcus of group b) have been assayed in genital samplings of 164 asymptomatic women (control group) and of 374 women suffering from abnormal vaginal secretions (vaginitis group). all these bacteria except group b streptococci were isolated in the vaginitis group more frequently than in the control group (p less than 0.01). the proportion of negative cultures for all tested bacteri ... | 1985 | 3934750 |
| influence of a constant magnetic field on antibody formation in experimentally immunized white rats. | 1985 | 3938764 | |
| energy-dispersive x-ray microanalysis of membrane-associated inclusions in hydrogenosomes isolated from trichomonas vaginalis. | energy-dispersive x-ray microprobe analysis of electron-dense inclusions in hydrogenosomes isolated from the aerotolerant anaerobic protozoal human parasite trichomonas vaginalis, bushby strain, indicated the presence of high levels of mg, p and ca. this suggested that divalent cation (e.g. ca2+ or mg2+) accumulation by hydrogenosomes may be important in the regulation of intracellular ion concentrations. | 1985 | 3005473 |
| heterogeneity of trichomonas vaginalis and discrimination among trichomonal isolates and subpopulations with sera of patients and experimentally infected mice. | the antibody response in trichomoniasis patients was examined with a variety of methodologies including enzyme-linked immunosorbent assays, indirect immunofluorescence, immunoblotting, and radioimmunoprecipitation-electrophoresis-autoradiography. based on enzyme-linked immunosorbent assay recognition of trichomonal isolates, sera from patients with trichomoniasis were categorized into reactive class i (ia, ib, and ic) and nonreactive class ii sera. a diminished ability to precipitate antibody-bi ... | 1985 | 2411656 |
| geographic variation among isolates of trichomonas vaginalis: demonstration of antigenic heterogeneity by using monoclonal antibodies and the indirect immunofluorescence technique. | although trichomonas vaginalis causes one of the most common sexually transmitted diseases, little is known about the antigenic variation of the parasite or about differences between strains in epidemiology or virulence. variation among isolates of t. vaginalis was investigated by using a panel of monoclonal antibodies, each reactive with different antigens, to test 88 isolates from diverse geographic areas of north america. all isolates of t. vaginalis reacted with at least one of the nine mono ... | 1985 | 2413147 |
| [vaginal microscopic evidence in women with symptoms of genital infection]. | vaginal living organisms have been defined as the most complete in human ecology. as many as 100 million colonies of microorganisms can live in a single ml of vaginal liquid. more common are the anaerobic types, particularly the lactobacillus and bacteroides. the low ph of the vagina, normally 4, is due to the high lactic acid content of the lactobacillus. pathogenic bacteria is inhibited at 4.5 ph. conversely, it proliferates at a high ph. the slightest alteration of this delicate equilib ... | 1985 | 12341331 |
| the vaginal contraceptive pill. | vaginal administration of oral contraceptives (ocs) has been shown to be an acceptable and effective method of contraception. the most widely tested vaginal contraceptive pill (vcp) contains 500 mcg of d-1 norgestrel and 50 mcg of ethinyl estradiol (ee) and is administered on the same 21-day schedule as the oral contraceptive. other combination ocs used vaginally include 1 mg of norethisterone plus 50 mcg of mestranol, 250 mcg of levonorgestrel plus 50 mcg of ee, and 2 mg of cyproterone acetat ... | 1985 | 12339969 |
| [frequency of trichomonas vaginalis infection in apparently healthy women in western santiago]. | the incidence of infection by trichomonas vaginalis in apparently healthy women was surveyed in the western area of santiago, chile, which comprised both urban and rural areas with medium- or low-income people. a retrospective review of the data of the cytopathology laboratory of san juan de dios hospital was conducted. the data contained all annual pap smears and epidemiological information about the presenting patients. during 1983, a total of 29,501 women had pap smears taken to detect sig ... | 1985 | 12320978 |
| [vaginitis due to gardnerella vaginalis in a university medical service]. | between august 1983 and june 1984, a total of 363 women were studied who had been referred to the medical service of the school of bacteriology and laboratory clinic of the university of antioquia, medellin, colombia, for obtaining vaginal cytology. they either had or did not have vaginal discharge. the smears were tested for gardnerella vaginalis and trichomonas vaginalis. the majority of the patients were in the third, fourth, and fifth decades of their lives: 51%, 24.5%, and 14%, respectiv ... | 1985 | 12292701 |
| trichomonas vaginalis in the prostate gland. | although the prostate gland is believed to serve as a parasite reservoir in trichomoniasis in men, and clinical association of trichomonads with prostatitis is common, there has been, to our knowledge, no unequivocal demonstration of trichomonas vaginalis within the prostate gland. using established immunoperoxidase procedures, we have positively identified trichomonads in the prostatic urethra, glandular lumina, submucosa, and stroma. foci of nonspecific acute and chronic inflammation, as well ... | 1986 | 2421689 |
| further studies on the surface charge of various strains of trichomonas vaginalis and tritrichomonas foetus. | the surface charge of three strains of trichomonas vaginalis and five strains of tritrichomonas foetus was determined by direct measurement of the mean cellular electrophoretic mobility (epm) of cells suspended in solutions of different ionic strength and ph. no differences were observed in the mean epm among the two species, although significant differences among the strains exist. strains that are more pathogenic to mouse, as measured using the subcutaneous assay, had a surface more negative. ... | 1986 | 2425974 |
| phenotypic variation and diversity among trichomonas vaginalis isolates and correlation of phenotype with trichomonal virulence determinants. | the extent and nature of heterogeneity among representative trichomonas vaginalis isolates were evaluated by flow cytofluorometry analysis. monoclonal antibody and monospecific antiserum to an immunodominant trichomonad surface glycoprotein with a molecular weight of 267,000 (267k glycoprotein) were used to evaluate fresh isolates (jhh and ru375) and long-term grown isolates (nyh286, ir78, and jh31a) of t. vaginalis. isolates nyh286, jh31a, and jhh were made up of heterogeneous staining (positiv ... | 1986 | 2426199 |
| detection of microtubules of the flagellate trichomonas vaginalis by monoclonal antibodies specific for beta-tubulin. | monoclonal antibodies specific for mammalian beta-tubulin recognized the microtubule cytoskeleton of the flagellated protozoon trichomonas vaginalis. of seven antibodies, two demonstrated the axostyle, costa, recurrent flagellum, and anterior flagella by indirect immunofluorescence microscopy. the remaining five stained a hazy reticular pattern in the cytoplasm of formaldehydefixed, detergent-extracted organisms. western immunoblots of whole t. vaginalis extracts treated with protease inhibitors ... | 1986 | 2432269 |
| respiration of trichomonas vaginalis. components detected by electron paramagnetic resonance spectroscopy. | 1986 | 3007142 | |
| [vulvovaginitis. etiopathogenesis and microbiological diagnosis]. | 1986 | 3008032 | |
| screening to reduce transmission of sexually transmitted diseases in semen used for artificial insemination. | the practice of artificial insemination by donor semen is increasing in the united states. many sexually transmitted organisms are found in semen, but screening procedures for the detection of these agents in donor semen have not been standardized. sexually transmitted organisms have been transmitted during artificial insemination by donor, and such transmission can cause local, disseminated, or fatal disease in the recipient woman and may harm the fetus or newborn. therefore, screening of both ... | 1986 | 3010110 |
| prevalence of genital pathogens among female prostitutes in new york city and in rotterdam. | the authors studied the prevalence of genital microorganisms among 300 female prostitutes in brothels in new york city and 60 female prostitutes attending a sexually transmitted diseases clinic in rotterdam, the netherlands. rates of isolation of neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, and ureaplasma urealyticum in the two cities were 9.3% and 8.3%, 25.3% and 16.6%, 57.3% and 74.9%, and 73% and 79%, respectively. trichomonas vaginalis was detected in 3.6% of new york pr ... | 1986 | 3012805 |
| [antiviral agents. 28. aliphatic substituted chlordihexylamino-1,3,5-triazine]. | the nucleophilic substitution of one chlorine atom in 2,4-dichloro-6-(dihexylamino)-1,3,5-triazine (1) by primary (2a-b) or secondary (2c) amines leads to the aliphatically substituted chloro-dihexylamino-1,3,5-triazines (3a-c) structures of type 3 may be characterized by the spectroscopic data. in the mass spectra, the complete degradation of the alkyl groupings is striking and manifested in a series of consecutive peaks with differences of 14 between each other. antiviral activity is exhibited ... | 1986 | 3013214 |
| glycerol, a metabolic end product of trichomonas vaginalis and tritrichomonas foetus. | glycerol was demonstrated as an end product of anaerobic glucose metabolism in trichomonas vaginalis and tritrichomonas foetus, produced in addition to acetate, h2, co2, and lactate or succinate. in t. vaginalis strain c-1, glycerol amounted to 16% of the fermentation products and was formed at an average rate of 38 nmol min-1 (mg protein)-1. corresponding figures for t. foetus strain kv1 were 7% and 4.8 nmol min-1 (mg protein)-1. the amounts of glycerol detected compensated almost exactly for t ... | 1986 | 3016535 |
| purine salvage enzymes in trichomonas vaginalis. | 1986 | 3020926 | |
| [sexually transmitted infections in patients with condylomata acuminata]. | 42 patients (26 males, 16 females) with genital and perianal warts were submitted to serological testing for hbv markers, anti hsv antibodies and syphilis. specimens were also collected for microscopic and cultural examination for neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, candida albicans and other pathogenic bacteria from urethra in men and from urethra, vagina and cervix in women. women had also cytologic examination from cervix with papanicolau method. we have found ... | 1986 | 3081835 |
| prevalence of nonspecific vaginitis and other cervicovaginal infections during the third trimester of pregnancy. | nonspecific vaginitis (nsv) is a clinical syndrome characterized by a decreased concentration of normal acidophilic döderlein flora in the vagina and increased concentration of nonacidophilic microorganisms, such as gardnerella vaginalis, anaerobic bacteria, and mycoplasmas. since several nsv-associated organisms are associated with complications of pregnancy, we studied the prevalence of nsv among 104 consecutive women during the third trimester of pregnancy. overall, 14 women (14%) had nsv by ... | 1986 | 3082020 |
| sexually transmissible infectious agents in sexually active and virginal asymptomatic adolescent girls. | sixty-eight sexually active and 52 virginal adolescent girls were evaluated for six sexually transmissible infectious agents: gardnerella vaginalis, ureaplasma urealyticum, mycoplasma hominis, chlamydia trachomatis, trichomonas vaginalis, and neisseria gonorrhoeae. there were significant differences between sexually active and virginal girls with respect to the prevalence of isolation of u urealyticum (75% v 33%, p less than .005), m hominis (27% v 10%, p less than .05), and c trachomatis (19% v ... | 1986 | 3083395 |
| microbiology of vaginal discharge in general practice. | three groups of women were examined by culture for gardnerella vaginalis and candida. group i consisted of 427 women, who complained spontaneously of vaginal discharge, group ii of 311 women who did not complain of vaginal discharge until questioned prior to gynaecological examination, and group iii of 100 women who denied vaginal discharge. groups i and ii also had cultures made for trichomonas vaginalis and neisseria gonorrhoica. in group i with spontaneous complaints the one-year prevalence r ... | 1986 | 3088694 |
| microflora in pregnancy. | 1986 | 3090295 | |
| polyamine biosynthesis in trichomonads. | trichomonas vaginalis, tritrichomonas foetus and trichomitus batrachorum grown in modified diamond's medium all had high concentrations of putrescine and lower concentrations of spermidine and spermine. ornithine decarboxylase (odc; ec 4.1.1.17) was detectable in all three species although at significantly different levels. trichomonas vaginalis had the highest activity (typically around 1.85 nmol min-1 (mg protein)-1), trichomitus batrachorum the lowest (0.11 nmol min-1 (mg protein)-1). the tri ... | 1986 | 3090433 |
| anaerobic pyruvate metabolism of tritrichomonas foetus and trichomonas vaginalis hydrogenosomes. | hydrogenosomes isolated from tritrichomonas foetus and trichomonas vaginalis fermented pyruvate to acetate, malate, h2, and co2 in an anaerobic process dependent on adp, pi, mg2+, and succinate. the extent to which pyruvate was carboxylated to malate by malate dehydrogenase (decarboxylating) rather than decarboxylated to acetate by pyruvate/ferredoxin oxidoreductase was dependent on pco2. the processes observed showed carbon and redox balances. the presence of an nadh/ferredoxin oxidoreductase a ... | 1986 | 3090435 |
| risk of transmission of sexually transmitted diseases by cryopreserved semen. | the increasing incidence of sexually transmitted diseases (std) involves the risk of the transmission of these diseases by the artificial insemination. therefore the cryotolerance of several microorganisms causing std and the efficiency of penicillin of the cryoprotective medium (cmp) against neisseria gonorrhoeae (n.g.) were investigated. the results have shown that penicillinase-producing n.g. (ppng), genital mycoplasmas and candida species survive the cryopreservation. trichomonas vaginalis c ... | 1986 | 3090904 |
| [heterogeneity of tubulins of parasitic protozoa]. | 1986 | 3092715 | |
| aminotransferase activities in trichomonas vaginalis. | a survey of aminotransferase activities present in a cell-free extract of the anaerobic protozoan, trichomonas vaginalis was performed. 2-oxoglutarate, oxaloacetate or phenylpyruvate acted as effective amino acceptors with tyrosine, phenylalanine, tryptophan, leucine, valine, isoleucine, aspartate, alanine, ornithine or lysine. arginine, serine, glutamine, glycine, beta-alanine and gamma-aminobutyrate were not active as amino donors. with pyruvate as acceptor, significant, yet low, activity was ... | 1986 | 3095639 |
| evaluation of new plastic envelope microbiology (pem) methods as adjuncts in the diagnosis of candida albicans and trichomonas vaginalis vaginitis. | 1986 | 3095702 | |
| inhibition of trichomonas vaginalis replication by the microtubule stabilizer taxol. | taxol, a plant alkaloid stabilizer of microtubules, inhibits in vitro the replication of the human pathogenic flagellate trichomonas vaginalis in a dose-dependent fashion. micromolar concentrations of the drug induce massive assembly of microtubules, resistant to antimicrotubule agents, and block the mitosis of the protozoa at an early stage preceding the formation of the spindle fibers and the depolymerization of the axostylar microtubules. some hypotheses involving the inactivation of the micr ... | 1986 | 2874220 |
| aspartate:2-oxoglutarate aminotransferase from trichomonas vaginalis: comparison with pig heart cytoplasmic enzyme. | the aspartate:2-oxoglutarate aminotransferase from the protozoon trichomonas vaginalis exists as a mixture of sub-forms of identical mr and amino acid composition, and of similar catalytic properties. the amino acid composition closely resembles that of aspartate aminotransferase from prokaryotic and vertebrate sources. some molecular and catalytic properties of the t. vaginalis aspartate aminotransferase are compared with those of the cytoplasmic pig heart enzyme. a major difference is in the a ... | 1986 | 2876843 |
| modulation of amino acid and 2-oxo acid pools in trichomonas vaginalis by aspartate aminotransferase inhibitors. | the amino acid pool sizes of trichomonas vaginalis are reported. alanine, glutamic acid, proline and leucine account for 72% of the measured amino acids. growth of t. vaginalis was unaffected by gostatin, an irreversible inhibitor of aspartate aminotransferase, when the enzyme activity within the cell had been completely inhibited and a specific elevation of the aspartate pool had occurred. in media lacking aspartate and glutamate, the amino acid substrates of the aspartate aminotransferase reac ... | 1986 | 2877395 |
| the possible role of trichomonas vaginalis as a "vector" for the spread of other pathogens. | 1986 | 2878896 | |
| [pathogen-host relations in sexually transmitted diseases]. | the host-parasite relationship between the major causative agents of sexually transmitted diseases, e.g. neisseria gonorrhoeae, treponema pallidum and trichomonas vaginalis, and their target cells in the urogenital tract can generally be studied in suitable cell and organ cultures. experience with n. gonorrhoeae predominantly gained from fallopian-tube organ cultures shows that there are several prerequisites for adherence as the first step of pathogenesis. they range from the type of bacterial ... | 1986 | 2878907 |
| bacterial flora of the cervix in women using different methods of contraception. | bacteriologic culture samples were taken from the cervix in three groups of 10 healthy, sexually active women using barrier contraception, oral contraceptives, or a levonorgestrel-releasing intrauterine contraceptive device. culture samples for candida albicans and trichomonas vaginalis were taken, a cytologic vaginal smear was obtained, and an amine sniff test was performed; these were in addition to a routine gynecologic examination. multiple bacteria were isolated from the cervix in women usi ... | 1986 | 3953700 |
| a new metabolic labelling medium for trichomonas vaginalis and tritrichomonas foetus using 35s methionine. | a metabolic labelling medium was devised for trichomonas vaginalis and tritrichomonas foetus utilizing 35s methionine. t. vaginalis cultured for 24h in the medium took up approximately 27% of the available label and increased greater than two fold in number. counts per microgram of protein were 32,555 +/- 10% between different strains or identical strains in different labelling runs. t. foetus took up approximately 5% of the available label and increased greater than two fold in 24h. this result ... | 1986 | 3485292 |
| etiology of cervical inflammation. | we studied the relationships of selected microbial, clinical, demographic, and behavioral variables to mucopurulent cervicitis in two clinical settings, a sexually transmitted disease clinic and a student health clinic. from each clinic, we studied a group of women referred for suspected mucopurulent cervicitis and a representative sample of other women attending the clinic. after the women were stratified by patient group and summary odds ratios for all groups were obtained, mucopurulent cervic ... | 1986 | 3485379 |
| trichomonas vaginalis: electrophoretic analysis and heterogeneity among isolates due to high-molecular-weight trichomonad proteins. | the protein composition of trichomonas vaginalis isolates was evaluated using one-dimensional and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography. at least two hundred intrinsically labeled and about 30 major extrinsically labeled proteins of molecular weights less than 120,000 were resolved by isoelectric focusing and electrophoresis. in general, long-term grown and fresh isolates of t. vaginalis gave similar fluorograms of the readily detectable prote ... | 1986 | 3485537 |
| tioconazole 2% cream in the treatment of trichomonas vaginalis or mixed vaginal infections. | twenty patients with documented t. vaginalis infections, six of whom were simultaneously infected with c. albicans and one with g. vaginalis, were treated topically (intravaginally) once daily with 5 g of 2% tioconazole vaginal cream (trosyd, pfizer) for 3 consecutive days in an open, non-comparative study. at the first follow-up visit (about 7 days post-treatment), 95% (19/20) of the patients were cured; 95% (18/19) of patients remained cured with respect to the baseline infection at the long-t ... | 1986 | 3485546 |
| isolation of trichomonas vaginalis: a simple diagnostic medium for use in developing countries. | 1986 | 3485756 | |
| growth and cytopathogenicity of trichomonas vaginalis in tissue cultures. | the primary purpose of this study was to identify the mammalian tissue cultures which were most suitable for investigations of the cytopathogenicity of trichomonas vaginalis. a recently isolated strain of the organism was inoculated into 15 different tissue cultures which were maintained in an appropriately modified growth medium. proliferation of the protozoon was accompanied by the progressive disintegration of cell culture monolayers. initial focal lesions consisting of detached cells and an ... | 1986 | 3486191 |
| in vitro drug susceptibility and doses of metronidazole required for cure in cases of refractory vaginal trichomoniasis. | there are currently no laboratory or clinical guidelines for the identification and treatment of disease caused by metronidazole-resistant strains of trichomonas vaginalis. fifty-three isolates of t. vaginalis from cases of refractory vaginitis in the united states (26 states) and canada were tested for aerobic and anaerobic metronidazole susceptibility, and after various dosages of metronidazole, the therapeutic outcomes were evaluated for 31 of these cases. the mean aerobic metronidazole susce ... | 1986 | 3486237 |
| ferredoxin-dependent reduction of nitroimidazole derivatives in drug-resistant and susceptible strains of trichomonas vaginalis. | the inhibitory effect of a range of nitroimidazole-derivatives on h2 production by metronidazole resistant (cdc-85) and susceptible (c1-nih) trichomonas vaginalis strains was investigated. the 2-, 4-, and 5-nitro-derivatives used had one-electron reduction potentials within the range -250 to -525 mv. nitroimidazole concentrations giving 50% inhibition of h2 production (kih2) for compounds with one-electron reduction potentials in the range -250 to -425 mv were found to be similar for both strain ... | 1986 | 3486660 |
| persistent trichomonas vaginalis infection due to a metronidazole-resistant strain. | 1986 | 3486706 | |
| incubation time, second blind passage, and cost considerations in the isolation of trichomonas vaginalis. | in a group of 247 symptomatic and asymptomatic women, 75 (30.3%) were found to harbour trichomonas vaginalis; 39 (52%) infections were detected by wet mount and 36 (48%) were detected by culture only. of the latter, 27 produces a positive culture in 3 days or less and 9 were positive, on average, at day 6. the break even cost of culture was $6.75 per patient. among 70 wet mount-negative, primary culture-negative specimens that received a blind passage, another 3 (4.2%) positive cultures were det ... | 1986 | 3487552 |
| vaginal trichomoniasis in sharkyia governorate. | 1986 | 3487598 | |
| metronidazole-resistant clinical isolates of trichomonas vaginalis have lowered oxygen affinities. | oxygen affinities of metronidazole susceptible and resistant isolates of the parasitic flagellate protozoon trichomonas vaginalis were determined by mass spectrometric methods. apparent o2km values for the respiration of non-proliferating cell suspensions were about 10-fold higher for metronidazole resistant strains than for the susceptible strains c1-nih or nyh-286. simultaneous monitoring of hydrogen evolution in the presence of increasing o2 tensions enabled apparent ki values for h2 to be de ... | 1986 | 3487729 |
| morphological studies of the cytotoxicity of trichomonas vaginalis to normal human vaginal epithelial cells in vitro. | in vitro cultured monolayers of normal human vaginal epithelial cells were incubated with axenic cultures of trichomonas vaginalis. two strains freshly isolated from patients with trichomoniasis and one strain that had been maintained in axenic culture for several years were studied. freshly isolated trichomonads showed amoeboid movements, adherence to epithelial cell surfaces, and were cytotoxic to epithelial cells in vitro. in contrast, the laboratory strain maintained for years in axenic cult ... | 1986 | 3488256 |
| [symptomatic and asymptomatic vaginitis. microbiological, clinical and therapeutic aspects]. | 1986 | 3488870 | |
| monoclonal antibody to a major glycoprotein immunogen mediates differential complement-independent lysis of trichomonas vaginalis. | an immunoglobulin g type 2a (igg2a) monoclonal antibody (mab), designated c20a3, which reacts with a highly immunogenic trichomonad membrane glycoprotein (approximately 270,000 daltons), produced complement-independent cytolysis of trichomonas vaginalis organisms. time- and temperature-dependent lysis of parasites was observed following incubation of washed, live t. vaginalis with certain concentrations of c20a3 igg. differential killing of trichomonal isolates and clones of a given isolate by c ... | 1986 | 3488964 |
| frequency and epidemiologic associations of different types of vaginitis in symptomatic women in greece. | 1986 | 3489617 | |
| the double-stranded rna in trichomonas vaginalis may originate from virus-like particles. | a linear 5.5-kilobase double-stranded rna, identified in many strains and isolates of the parasitic protozoan trichomonas vaginalis in a previous study, is found largely intact in ribonuclease-treated homogenates of the parasite. it can be pelleted with membranes from the homogenate at 12,500 x g and further purified in cscl buoyant density-gradient centrifugations. the purified sample contains the double-stranded rna as well as one major protein with an estimated molecular mass of 85 kda in nad ... | 1986 | 3489942 |