Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| evaluation of vaccinal effectiveness of preparations containing membrane antigens of leishmania (l.) amazonensis in experimental cutaneous leishmaniasis model. | american tegumentary leishmaniasis (atl) is considered a neglected disease, for which an effective vaccine or an efficient diagnosis is not yet available and whose chemotherapeutic arsenal is threatened by the emergence of resistance by etiological agents such as leishmania amazonensis. atl is endemic in poor countries and has a high incidence in brazil. vaccines developed from native parasite fractions have led to the identification of defined antigenic subunits and the development of vaccine a ... | 2017 | 28433944 |
| effect of 1,2,3-triazole salts, non-classical bioisosteres of miltefosine, on leishmania amazonensis. | here, we report the effect of new non-classical bioisosteres of miltefosine on leishmania amazonensis. fifteen compounds were synthesized and the compound dhmtac, containing an acetate anion, a side chain of 10 carbon atoms linked to n-1 and a methyl group linked to n-3, showed high and selective biological activity against l. amazonensis. on the intracellular amastigotes, stages of the parasite related to human disease, the ic50 values were near or similar to the 1.0μm (0.9, 0.8 and 1.0μm on l. ... | 2017 | 28433512 |
| pcr-based identification of trypanosoma lewisi and trypanosoma musculi using maxicircle kinetoplast dna. | trypanosoma lewisi, transmitted by rat fleas, is a widespread pathogen specific to rats with records of human infection cases. its closely related species with global distribution, trypanosoma musculi, is transmitted between mice by ingestion of infected fleas. these trypanosomes are of similar morphology, making it difficult to distinguish them by microscopy. in this study, we have developed a rapid, sensitive and reliable pcr method for the diagnosis of t. lewisi and t. musculi. the t. lewisi- ... | 2017 | 28427957 |
| effect of piplartine and cinnamides on leishmania amazonensis, plasmodium falciparum and on peritoneal cells of swiss mice. | plants of the piperaceae family produce piplartine that was used to synthesize the cinnamides. | 2017 | 28415906 |
| resolvin d1 drives establishment of leishmania amazonensis infection. | previous studies have indicated that the balance between different eicosanoids reflect the intensity of the inflammatory profile in patients with tegumentary leishmaniasis. more recently, pro-resolution lipid mediators have been shown to play critical roles in dampening pathological inflammatory processes to reestablish homeostasis in a diverse range of experimental settings. among these lipid mediator, resolvins from d series have been described as potent anti-inflammatory and immunomodulatory ... | 2017 | 28393908 |
| a proteomic road to acquire an accurate serological diagnosis for human tegumentary leishmaniasis. | diagnostic tools are important for clinical management and epidemiological evaluation of tegumentary (tl) and visceral (vl) leishmaniasis. serology is not frequently used for the diagnosis of the tl form because low antibody titers and cross-reaction with vl. therefore, it is crucial to identify specific and immunogenic antigens from species associated with the tl form. here we employed a proteomic approach coupled to an in silico analysis and identified the most abundant and immunogenic protein ... | 2017 | 27262223 |
| leishmania infection inhibits macrophage motility by altering f-actin dynamics and the expression of adhesion complex proteins. | leishmania is an intracellular protozoan parasite that causes a broad spectrum of clinical manifestations, ranging from self-healing skin lesions to fatal visceralizing disease. as the host cells of choice for all species of leishmania, macrophages are critical for the establishment of infections. how macrophages contribute to parasite homing to specific tissues and how parasites modulate macrophage function are still poorly understood. in this study, we show that leishmania amazonensis infectio ... | 2017 | 27641840 |
| a vaccine composed of a hypothetical protein and the eukaryotic initiation factor 5a from leishmania braziliensis cross-protection against leishmania amazonensis infection. | in the present study, two proteins cloned from leishmania braziliensis species, a hypothetical protein (lbhyp) and the eukaryotic initiation factor 5a (eif5a), were evaluated to protect balb/c mice against l. amazonensis infection. the animals were immunized with the antigens, either separately or in combination, using saponin as an immune adjuvant in both cases. spleen cells from vaccinated and later infected mice produced significantly higher levels of protein and parasite-specific ifn-γ, il-1 ... | 2017 | 27693018 |
| antileishmanial activity of a calophyllum brasiliense leaf extract. | calophyllum brasiliense (clusiaceae) is a tree that grows mainly in the atlantic forest in brazil. its leaves possess antibacterial activity, cytotoxic activity against certain tumor cell lines, and antimicrobial activity in balb/c mice infected with leishmania (leishmania) amazonensis.aiming to identify ultrastructural changes and dna fragmentation in leishmania (viannia) braziliensis, promastigotes were treated with a concentration of the dichloromethane extract and coumarin (-) mammea a/bb fr ... | 2017 | 27224267 |
| new primers for detection of leishmania infantum using polymerase chain reaction. | leishmania infantum causes visceral leishmaniasis (vl) in the new world. the diagnosis of vl is confirmed by parasitological and serological tests, which are not always sensitive or specific. our aim was to design new primers to perform a polymerase chain reaction (pcr) for detecting l. infantum. sequences of the minicircle kinetoplast dna (kdna) were obtained from genbank, and the flc2/rlc2 primers were designed. samples of dna from l. infantum, leishmania amazonensis, leishmania braziliensis, ... | 2017 | 26603223 |
| in vitro effects of the neolignan 2,3-dihydrobenzofuran against leishmania amazonensis. | leishmaniasis is an infectious disease complex caused by protozoa from the leishmania genus, which presents a broad spectrum of clinical manifestations: cutaneous, mucocutaneous and visceral forms. the current treatments are unsatisfactory considering that few drugs are available and present some level of toxicity. many lignans and neolignans have been used for the development of new antileishmania drugs. the capability in vitro of the neolignan 2,3-dihydrobenzofuran (2,3-dbf), a commonly found ... | 2017 | 27398818 |
| a3k2a3-induced apoptotic cell death of leishmania amazonensis occurs through caspase- and atp-dependent mitochondrial dysfunction. | leishmaniasis is a neglected tropical disease that affects millions of people worldwide. current therapies mainly rely on antimonial drugs that are inadequate because of their high toxicity and increased drug resistance. an urgent need exists to discover new, more effective, more affordable, and more target-specific drugs. pathways that are associated with apoptosis-like cell death have been identified in unicellular eukaryotes, including protozoan parasites. in the present study, we studied the ... | 2017 | 27761752 |
| exploration of the imidazo[1,2-b]pyridazine scaffold as a protein kinase inhibitor. | 3,6-disubstituted imidazo[1,2-b]pyridazine derivatives were synthesized to identify new inhibitors of various eukaryotic kinases, including mammalian and protozoan kinases. among the imidazo[1,2-b]pyridazines tested as kinase inhibitors, several derivatives were selective for dyrks and clks, with ic50 < 100 nm. the characterization of the kinome of several parasites, such as plasmodium and leishmania, has pointed out profound divergences between protein kinases of the parasites and those of the ... | 2017 | 27721154 |
| antiprotozoal and antioxidant alkaloids from alternanthera littoralis. | five alkaloids, in addition to hydroxytyrosol and uridine, were isolated from aerial parts of alternanthera littoralis p. beauv. among the isolated compounds, alternamide a was an unusual tricyclic alkaloid with a bridged benzoazepine core. all isolated alkaloids have a catechol moiety, indicating a possible common biosynthetic route. their structures were established by 1d and 2d nmr spectroscopy in combination with extensive tandem ms experiments by collisional induced dissociation (cid). the ... | 2017 | 27889243 |
| the cytotoxic activity of miltefosine against leishmania and macrophages is associated with dynamic changes in plasma membrane proteins. | in this study, we combined electron paramagnetic resonance (epr) spectroscopy with an analysis of biophysical cellular parameters to study the mechanisms underlying the in vitro anti-leishmanial activity of miltefosine (mt). a thiol-specific spin label attached to membrane-bound proteins of leishmania amazonensis and peritoneal macrophages indicated that mt may bind to plasma membrane proteins in large quantities via a detergent-like action and cause structural changes associated with a marked i ... | 2017 | 27773565 |
| anti-leishmania amazonensis activity of serjania lethalis a. st.-hil. | extracts of serjania lethalis a. st.-hil leaves and stems were tested in order to identify potential agents against leishmania amazonensis. the hexane fraction (hf) and dichloromethane subfractions (ddf and mdf) showed leishmanicidal effect. the anti-promastigote ic50 values were 10.29 (hf), 11.41 (ddf) and 28.33μg⁄ml (mdf); whereas those against amastigote were 7.2 (hf), 8.1 (ddf) and 6.5μg⁄ml (mdf). among the fractions and subfractions assayed, only hf altered the cell cycle of the parasite, i ... | 2017 | 27793725 |
| consequences of acute oxidative stress in leishmania amazonensis: from telomere shortening to the selection of the fittest parasites. | leishmaniasis is a spectrum of diseases caused by parasites of the genus leishmania that affects millions of people around the world. during infection, the parasites use different strategies to survive the host's defenses, including overcoming exposure to reactive oxidant species (ros), responsible for causing damage to lipids, proteins and dna. this damage especially affects telomeres, which frequently results in genome instability, senescence and cell death. telomeres are the physical ends of ... | 2017 | 27836509 |
| use of recombinant antigens for sensitive serodiagnosis of american tegumentary leishmaniasis caused by different leishmania species. | american tegumentary leishmaniasis (atl) (also known as cutaneous leishmaniasis [cl]) is caused by various species of protozoa of the genus leishmania the diagnosis is achieved on a clinical, epidemiological, and pathological basis, supported by positive parasitological exams and demonstration of leishmanin delayed-type hypersensitivity. serological assays are not routinely used in the diagnosis because many are considered to have low sensitivity and the particular leishmania species causing the ... | 2017 | 27927927 |
| nanoscaled hydrated antimony (v) oxide as a new approach to first-line antileishmanial drugs. | coordination compounds of pentavalent antimony have been, and remain, the first-line drugs in leishmaniasis treatment for >70 years. molecular forms of sb (v) complexes are commercialized as sodium stibogluconate (pentostam(®)) and meglumine antimoniate (ma) (glucantime(®)). ever-increasing drug resistance in the parasites limits the use of antimonials, due to the low drug concentrations being administered against high parasitic counts. sb(5+) toxicity provokes severe side effects during treatme ... | 2017 | 28008252 |
| treatment of leishmania (leishmania) amazonensis-infected mice with a combination of a palladacycle complex and heat-killed propionibacterium acnes triggers protective cellular immune responses. | palladacycle complex dppe 1.2 was previously reported to inhibit the in vitro and in vivo infection by leishmania (leishmania) amazonensis. the aim of the present study was to compare the effect of dppe 1.2, in association with heat-killed propionibacterium acnes, on l. (l.) amazonensis infection in two mouse strains, balb/c and c57bl/6, and to evaluate the immune responses of the treated animals. foot lesions of l. (l.) amazonensis-infected mice were injected with dppe 1.2 alone, or associated ... | 2017 | 28321209 |
| biological activities of croton palanostigma klotzsch. | different species of croton are used in traditional amazonian medicine. among the popular uses are treatment of bacterial diseases, poorly healing wounds and fevers. | 2017 | 26246738 |
| bioactive compounds of aspergillus terreus-f7, an endophytic fungus from hyptis suaveolens (l.) poit. | the compounds terrein (1), butyrolactone i (2), and butyrolactone v (3) were isolated from the ethyl acetate extract (etoac) of the endophytic fungus aspergillus terreus-f7 obtained from hyptis suaveolens (l.) poit. the extract and the compounds presented schistosomicidal activity against schistosoma mansoni; at 100 µg/ml for etoac extract, 1297.3 µm for compound 1, 235.6 µm for compound 2, and 454.1 µm for compound 3, they killed 100% of the parasites after 72 h of treatment. compounds 1, 2, an ... | 2017 | 28243983 |
| comparative genomics of canine-isolated leishmania (leishmania) amazonensis from an endemic focus of visceral leishmaniasis in governador valadares, southeastern brazil. | leishmaniasis is a highly diverse group of diseases caused by kinetoplastid of the genus leishmania. these parasites are taxonomically diverse, with human pathogenic species separated into two subgenera according to their development site inside the alimentary tract of the sand fly insect vector. the disease encompasses a variable spectrum of clinical manifestations with tegumentary or visceral symptoms. among the causative species in brazil, leishmania (leishmania) amazonensis is an important e ... | 2017 | 28091623 |
| leishmaniasis recidivans in ethiopia: cutaneous and mucocutaneous features. | cutaneous leishmaniasis (cl) is endemic in ethiopia. an unusual clinical form of this disease is leishmaniasis recidivans (lr), a prolonged, relapsing form of cutaneous leishmaniasis resembling tuberculosis of the skin that may persist for many years with a chronic and relapsing course. this rare variant has been shown to be caused by leishmania tropica species in the old world and by leishmania braziliensis, leishmania amazonensis, leishmania panamensis, and leishmania guyanensis in the new wor ... | 2017 | 28141598 |
| the anti-trichomonas vaginalis phloroglucinol derivative isoaustrobrasilol b modulates extracellular nucleotides hydrolysis. | trichomonas vaginalis causes trichomoniasis, a neglected sexually transmitted disease. due to severe health consequences and treatment failure, new therapeutic alternatives are crucial. phloroglucinols from southern brazilian hypericum species demonstrated anti-t. vaginalis and anti-leishmania amazonensis activities. the modulation of biochemical pathways involved in the control of inflammatory response by ectonucleotidases, ntpdase and ecto-5'-nucleotidase, represents new targets for combating ... | 2017 | 28390095 |
| glycosylated metal chelators as anti-parasitic agents with tunable selectivity. | trypanosoma cruzi and leishmania amazonensis are the causative agents of chagas' disease and leishmaniasis, respectively. these conditions affect millions of people worldwide, especially in developing countries. as such, there is an urgent need for novel, efficient and cost-effective treatments for these diseases, given the growing resistance and side-effects of current therapies. this work details the synthesis and evaluation of the anti-parasitic activity of novel amino- and iminopyridyl metal ... | 2017 | 28382355 |
| exploring the association of surface plasmon resonance with recombinant mhc:ig hybrid protein as a tool for detecting t lymphocytes in mice infected with leishmania (leishmania) amazonensis. | a surface plasmon resonance- (spr-) based recognition method applying h-2 l(d):ig/peptides complexes for ex vivo monitoring cellular immune responses during murine infection with leishmania (leishmania) amazonensis is described. lymphocytes from lesion-draining popliteal lymph nodes were captured on a carboxylated sensor chip surface previously functionalized with h-2 l(d):ig (dimerx) protein bound to synthetic peptides derived from the cooh-terminal region of cysteine proteinase b of l. (l.) am ... | 2017 | 28373990 |
| serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in indigenous reserves in the brazilian amazon region. | leishmaniasis is endemic to the northern, northeastern, central-western, and southeastern regions of brazil. we aimed to assess the epidemiological situation of leishmaniasis in humans and dogs in indigenous villages located in the states of mato grosso and tocantins using a serological survey conducted in may 2011. | 2017 | 28327803 |
| antileishmanial activity of handroanthus serratifolius (vahl) s. grose (bignoniaceae). | this study aimed to evaluate the leishmanicidal activity of ethanol extract, fractions, and isolated substance from handroanthus serratifolius against leishmania amazonensis. furthermore, this activity was related to cytotoxicity, and the selectivity index was determined. the ethanol extract was obtained by maceration of the stem powder, and the extract was subjected to fractionation on chromatographic column. the lapachol was obtained by acid base extraction followed by purification in chromato ... | 2017 | 28286535 |
| a chimera containing cd4+ and cd8+ t-cell epitopes of the leishmania donovani nucleoside hydrolase (nh36) optimizes cross-protection against leishmania amazonesis infection. | the leishmania donovani nucleoside hydrolase (nh36) and nh a34480 of leishmania amazonensis share 93% of sequence identity. in mice, the nh36 induced protection against visceral leishmaniasis is mediated by a cd4+ t cell response against its c-terminal domain (f3). besides this cd4+ th1 response, prevention and cure of l. amazonensis infection require also additional cd8+ and regulatory t-cell responses to the nh36 n-terminal (f1 domain). we investigated if mice vaccination with f1 and f3 domain ... | 2017 | 28280494 |
| leishmania (leishmania) amazonensis induces macrophage mir-294 and mir-721 expression and modulates infection by targeting nos2 and l-arginine metabolism. | leishmania (leishmania) amazonensis is an intracellular protozoan parasite responsible for the cutaneous leishmaniasis. the parasite replicates inside mammalian macrophage to establish infection. host-pathogen interactions result in microrna-mediated post-transcriptional regulation of host genes involved in inflammatory immune response. we analyzed macrophage mirna profiles during l. (l.) amazonensis infection. the regulation of macrophage mirna expression by the parasite correlates with/depends ... | 2017 | 28276497 |
| topical tamoxifen in the therapy of cutaneous leishmaniasis. | the aims of the present work were to test the effect of tamoxifen administered topically and the therapeutic efficacy of tamoxifen and pentavalent antimonial combinations in an experimental model of cutaneous leishmaniasis. balb/c mice infected with a luciferase expressing line of leishmania amazonensis were treated with topical tamoxifen in two different formulations (ethanol or oil-free cream) as monotherapy or in co-administration with pentavalent antimonial. treatment efficacy was evaluated ... | 2017 | 28274283 |
| induction of early autophagic process on leishmania amazonensis by synergistic effect of miltefosine and innovative semi-synthetic thiosemicarbazone. | drug combination therapy is a current trend to treat complex diseases. many benefits are expected from this strategy, such as cytotoxicity decrease, retardation of resistant strains development, and activity increment. this study evaluated in vitro combination between an innovative thiosemicarbazone molecule - bzts with miltefosine, a drug already consolidated in the leishmaniasis treatment, against leishmania amazonensis. cytotoxicity effects were also evaluated on macrophages and erythrocytes. ... | 2017 | 28270805 |
| cutaneous leishmaniasis in french guiana: revising epidemiology with pcr-rflp. | the development of polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) technique for species identification among patients presenting leishmaniasis allowed to better determine the main circulating species in french guiana. | 2017 | 28265182 |
| chemical composition, antiprotozoal and cytotoxic activities of indole alkaloids and benzofuran neolignan of aristolochia cordigera. | this is a comparative study on the intraspecific chemical variability of aristolochia cordigera species, collected in two different regions of brazil, biome cerrado (semiarid) and biome amazônia (coastal). the use of gc-ms and statistical methods led to the identification of 56 compounds. a higher percentage of palmitone and germacrene-d in the hexanes extracts of the leaves of plants from these respective biomes was observed. phytochemical studies on the extracts led to the isolation and identi ... | 2017 | 28264205 |
| differences in the detection of brdu/edu incorporation assays alter the calculation for g1, s, and g2 phases of the cell cycle in trypanosomatids. | trypanosomatids are the etiologic agents of various infectious diseases in humans. they diverged early during eukaryotic evolution and have attracted attention as peculiar models for evolutionary and comparative studies. here, we show a meticulous study comparing the incorporation and detection of the thymidine analogs brdu and edu in leishmania amazonensis, trypanosoma brucei, and trypanosoma cruzi to monitor their dna replication. we used brdu- and edu-incorporated parasites with the respectiv ... | 2017 | 28258618 |
| metacaspase-binding peptide inhibits heat shock-induced death in leishmania (l.) amazonensis. | leishmania (leishmania) amazonensis is an important agent of cutaneous leishmaniasis in brazil. this parasite faces cell death in some situations during transmission to the vertebrate host, and this process seems to be dependent on the activity of metacaspase (mca), an enzyme bearing trypsin-like activity present in protozoans, plants and fungi. in fact, the association between mca expression and cell death induced by different stimuli has been demonstrated for several leishmania species. regula ... | 2017 | 28252649 |
| cytokines and microbicidal molecules regulated by il-32 in thp-1-derived human macrophages infected with new world leishmania species. | interleukin-32 (il-32) is expressed in lesions of patients with american tegumentary leishmaniasis (atl), but its precise role in the disease remains unknown. | 2017 | 28241012 |
| anti-leishmania activity of essential oil of myracrodruon urundeuva (engl.) fr. all.: composition, cytotoxity and possible mechanisms of action. | myracrodruon urundeuva (engl.) fr. all., commonly known as "aroeira-do-sertão", is a medicinal plant from anacardiaceae family. in this study, the chemical composition of m. urundeuva essential oil (mueo) was evaluated by gas chromatography-mass spectrometry (gc-ms), as well as its anti-leishmania potential, cytotoxicity, and macrophage activation capability as possible antiprotozoal mechanism of action were assessed. fourteen compounds were identified, which constituted 94.87% of total oil comp ... | 2017 | 28189487 |
| n-acetyl-cysteine inhibits liver oxidative stress markers in balb/c mice infected with leishmania amazonensis. | leishmaniasis is a parasitosis caused by several species of the genus leishmania. these parasites present high resistance against oxidative stress generated by inflammatory cells. | 2017 | 28177049 |
| leishmania in synanthropic rodents (rattus rattus): new evidence for the urbanization of leishmania (leishmania) amazonensis. | this study aimed to detect parasites from leishmania genus, to determine the prevalence of anti-leishmania spp. antibodies, to identify circulating species of the parasite, and to determine epidemiological variables associated with infection in rats caught in urban area of londrina, paraná, brazil. animal capture was carried out from may to december 2006, serological and molecular methods were performed. dna was extracted from total blood, and nested-pcr, targeting ssu rrna from leishmania genus ... | 2017 | 28177041 |
| leishmania amazonensis and leishmania chagasi: in vitro leishmanicide activity of virola surinamensis (rol.) warb. | virola species have been used in traditional medicine as healing in skin infections. from v. surinanensis oil were isolated several sesquiterpene as the nerolidol which showed activity against species of leishmania. the current study aimed to evaluate the leishmanicide activity and toxicity of extracts, fractions and surinamesin obtained from leaves of virola surinamensis. hexane, ethyl acetate, and methanol extracts were obtained from powder of dry leaves of v. surinamensis. the hexane and ethy ... | 2017 | 28174103 |
| in vitro and in vivo antimalarial potential of oleoresin obtained from copaifera reticulata ducke (fabaceae) in the brazilian amazon rainforest. | in view of the wide variety of the flora of the amazon region, many plants have been studied in the search for new antimalarial agents. copaifera reticulata is a tree distributed throughout the amazon region which contains an oleoresin rich in sesquiterpenes and diterpenes with β-caryophyllene as the major compound. the oleoresin has demonstrated antiparasitic activity against leishmania amazonensis. because of this previously reported activity, this oleoresin would be expected to also have anti ... | 2017 | 28160850 |
| design and synthesis of a new series of 3,5-disubstituted isoxazoles active against trypanosoma cruzi and leishmania amazonensis. | chagas disease and leishmaniasis are neglected tropical diseases (ntds) endemic in developing countries. although there are drugs available for their treatment, efforts on finding new efficacious therapies are continuous. the natural lignans grandisin (1) and veraguensin (2) show activity against trypomastigote t. cruzi and their scaffold has been used as inspiration to design new derivatives with improved potency and chemical properties. we describe here the planning and microwave-irradiated sy ... | 2017 | 28152426 |
| effect of clinically approved hdac inhibitors on plasmodium, leishmania and schistosoma parasite growth. | malaria, schistosomiasis and leishmaniases are among the most prevalent tropical parasitic diseases and each requires new innovative treatments. targeting essential parasite pathways, such as those that regulate gene expression and cell cycle progression, is a key strategy for discovering new drug leads. in this study, four clinically approved anti-cancer drugs (vorinostat, belinostat, panobinostat and romidepsin) that target histone/lysine deacetylase enzymes were examined for in vitro activity ... | 2017 | 28107750 |
| perifosine mechanisms of action in leishmania species. | here the mechanism by which perifosine induced cell death in leishmania donovani and leishmania amazonensis is described. the drug reduced leishmania mitochondrial membrane potential and decreased cellular atp levels while increasing phosphatidylserine externalization. perifosine did not increase membrane permeabilization. we also found that the drug inhibited the phosphorylation of akt in the parasites. these results highlight the potential use of perifosine as an alternative to miltefosine aga ... | 2017 | 28096161 |
| high dilutions of antimony modulate cytokines production and macrophage - leishmania (l.) amazonensis interaction in vitro. | in previous results mice treated with high dilutions of antimony presented reduction of monocyte migration to the site of infection with increase in b lymphocytes population in the local lymph node. | 2017 | 28092793 |
| vector competence of lutzomyia cruzi naturally demonstrated for leishmania infantum and suspected for leishmania amazonensis. | corumbá city is one of the oldest visceral leishmaniasis-endemic foci in the state of mato grosso do sul, brazil, where the transmission of leishmania infantum has been attributed to lutzomyia cruzi aiming at investigating the parameters of the vectorial capacity of lu. cruzi for l. infantum, a project was undertaken in this city. among these parameters, vector competence was investigated and the results obtained are reported herein. of the 12 hamsters exposed to feed wild-caught female sandflie ... | 2017 | 28077746 |
| phenotypic diversity and selection maintain leishmania amazonensis infectivity in balb/c mouse model. | leishmania are protozoan parasites that show remarkable diversity, as revealed by the various clinical forms of leishmaniasis, which can range from mild skin lesions to severe metastatic cutaneous/mucosal lesions. the exact nature and extent of leishmania phenotypic diversity in establishing infection is not fully understood. in order to try to understand some aspects of this diversity, we subcutaneously infected balb/c mice with first and second generation subclones of a l. amazonensis strain i ... | 2017 | 28076468 |
| development and evaluation of zinc phthalocyanine nanoemulsions for use in photodynamic therapy for leishmania spp. | photodynamic therapy (pdt) combines light with photosensitizers (ps) for production of reactive oxygen species (ros) that can kill infectious microorganisms such as bacteria, fungi and protozoa. the application of nanotechnology has enabled the advancement of pdt because many ps are insoluble in water, necessitating a nanocarrier as a physiologically acceptable carrier. nanoemulsions are efficient nanocarriers for solubilizing liposoluble drugs, like the ps, in water. cutaneous (cl) and mucocuta ... | 2017 | 28071592 |
| in vitro antiprotozoal activity of (s)-cis-verbenol against leishmania spp. and trypanosoma cruzi. | (s)-cis-verbenol, a monoterpene frequently found as a component of essential oils, was assayed against leishmania amazonensis, leishmania infantum, leishmania brasiliensis and against two strains of trypanosoma cruzi. the cytotoxicity of the compound was also assayed against human fibroblast cells using a colorimetric method. benznidazole was used as reference drug against t. cruzi and amphotericin b was used against leishmania spp. the compound showed good activity against the trypanosomes, bei ... | 2017 | 28062234 |
| a recombinant fusion protein displaying murine and human mhc class i- and ii-specific epitopes protects against leishmania amazonensis infection. | tegumentary leishmaniasis (tl) constitutes a major public health problem with significant morbidity worldwide. synthetic peptide-based vaccines are attractive candidates to protect against leishmaniasis, since t cell-specific epitopes can be delivery to antigen-presenting cells, leading to the generation of a th1 cell-mediated immunity. in this context, the present study aims to evaluate the immunogenicity and protective efficacy of a vaccine composed of major histocompatibility complex class i ... | 2017 | 28049560 |
| oligopeptidase b and b2: comparative modelling and virtual screening as searching tools for new antileishmanial compounds. | leishmaniasis are diseases caused by parasites of the genus leishmania and transmitted to humans by the bite of infected insects of the subfamily phlebotominae. current drug therapy shows high toxicity and severe adverse effects. recently, two oligopeptidases (opbs) were identified in leishmania amazonensis, namely oligopeptidase b (opb) and oligopeptidase b2 (opb2). these opbs could be ideal targets, since both enzymes are expressed in all parasite lifecycle and were not identified in human. th ... | 2017 | 28031079 |
| antileishmanial activity of verbascoside: selective arginase inhibition of intracellular amastigotes of leishmania (leishmania) amazonensis with resistance induced by lps plus ifn-γ. | verbascoside is the main component of the traditional medicinal plants that were used against protozoa parasites that cause malaria and leishmaniasis. previously, we have described verbascoside inhibition of leishmania amazonensis arginase as well as its antileishmanial action against extracellular promastigotes. in this study, we have assessed arginase parasite inhibition in intracellular amastigotes. in addition, we verified whether verbascoside can influence the host defense against the paras ... | 2017 | 28017773 |
| leishmanicidal activity of the alkaloid-rich fraction from guatteria latifolia. | leishmaniasis is caused by protozoan parasites belonging to the genus leishmania and includes cutaneous, mucocutaneous and visceral clinical forms. the drugs currently available for leishmaniasis treatment are pentavalent antimonials, amphotericin b and miltefosine, which present high toxicity, elevated cost and development of parasite resistance. the natural products constitute an important source of substances with leishmanicidal potential. here we evaluated in vitro the anti-leishmania amazon ... | 2017 | 28011169 |