Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| characterization of plasma membrane adenosine triphosphatase of neurospora crassa. | it has been proposed (slayman, c.l., long w.s., and lu, c.y.-h. (1973) j. membr. biol. 14, 305--338) that in neurospora crassa, a plasma membrane atpase functions to pump h+ ions out of the cell, thereby generating an electrochemical gradient that can drive transport processes. using the concanavalin a method of scarborough (scarborough g.a. (1975)j. biol. chem. 250, 1106--1111), we have prepared plasma membranes of neurospora and have deomonstrated that they do contain a distinct atpase activit ... | 1977 | 16897 |
| biosynthesis of glycogen in neurospora crassa. existence of a glucoproteic intermediate in the initiation process. | a soluble enzyme preparation (20,000 x g supernatant fraction), prepared from the mycelia of wild-type neurospora crassa, was capable of transferring [14c]glucose from udp-[14c]glucose into both trichloroacetic acid (tca)-soluble and tca-insoluble macromolecule products in the absence of added primer. these reactions did not require either high concentrations of salts or any other chemical reagents. two labeled products were formed; one was a glycogen-like polysaccharide and the other was a glyc ... | 1977 | 19440 |
| amino acid replacements resulting from suppression and missense reversion of a chain-terminator mutation in neurospora. | the neurospora crassa super-suppressor mutation, ssu-1, suppresses the auxotrophic phenotype of the mutant am(17) by inserting tyrosine at residue 313 of nadp-specific glutamate dehydrogenase, a position occupied in the wild type by glutamate. two classes of am(17) revertants due to further mutation within the am gene have, respectively, tyrosine and leucine at residue 313. these replacements are consistent with a chain-terminating codon in am(17) of either the amber (uag) or the ochre type (uaa ... | 1977 | 18380 |
| energy-linked potassium uptake by mitochondria from wild-type and poky strains of neurospora crassa. | mitochondria from neurospora crassa, like mammalian mitochondria, carry out rapid, energy-linked k+ uptake and h+ release in the presence of valinomycin. the maximal rate of k+ uptake was about 1.0 mumol/mg of mitochondrial protein per min and was seen at valinomycin concentrations in the range of 100 to 200 mug per mg of mitochondrial protein and at k+ concentrations of 4 mm or above. uptake could be supported either by substrate oxidation or by adenosine 5'-triphosphate (atp), and was inhibite ... | 1977 | 138675 |
| composition and photoinduced biosynthesis of the carotenoids of a protoplast-like neurospora crassa "slime" mutant. | a spheroplast-like "slime" mutant of neurospora crassa (lacking a rigid cell wall) was found to synthesize an identical spectrum of carotenoids as wild type strains except for beta-carotene. furthermore strict photoregulation of the biosynthesis of these pigments as well as the characteristics of photoinduced carotenogenesis were also nearly identical in the mutant and in the wild type. | 1977 | 138402 |
| control of the production and partial characterization of repressible extracellular 5'-nucleotidase and alkaline phosphatase in neurospora crass. | a new species of orthophosphate repressible extracellular 5'-nucleotidase (5'-ribonucleotide phosphohydrolase, ec 3.1.3.5) was found to be released into mycelial culture media when a wild type strain of neurospora crassa was grown on limiting amounts of phosphate. the production of 5'-nucleotidase and extracellular acid and alkaline phosphatase was inhibited by the addition of rifampicin when it was added at the later stage of mycelial growth, but not when it was added at a very early stage. the ... | 1977 | 137748 |
| thymine 7-hydroxylase from neurospora crassa. substrate specificity studies. | a partially purified preparation of thymine 7-hydroxylase (thymine, 2-oxoglutarate : oxygen oxidoreductase (7-hydroxylating), ec 1.14.11.6) from neurospora crassa was incubated with a number of pyrimidines chemically related to tyymine. 1. pyrimidines with oxygen or sulfur substituents on atoms nos. 2 and 4 as well as an alkyl group on atom nos. 1 or 5 were substrates. 2. km values were determined for 1-methyluracil, 1-ethyluracil, thymine, 6-azathymine, 1-methylthymine, 1-ethylthymine, 5-formyl ... | 1977 | 139930 |
| role of lipids in the neurospora crassa membrane. i. influence of fatty acid composition on membrane lipid phase transitions. | the relationship between lipid composition and phase transition was investigated by differential scanning calorimetry for intact and membrane phospholipid extracts of wild-type (w/t) and the cel-(tw 40) mutant of neurospora crassa. the cel-(tw 40) mutant (grown on minimal, sucrose medium supplemented with tween 40 at approximately 34 degrees c) had approximately twice the saturated fatty acid content of w/t organisms grown at approximately 22 degrees c. the gel-liquid crystal phase transitions o ... | 1977 | 140245 |
| lecithin requirement for the sporulation process in neurospora crassa. | reversible inhibition of conidiogenesis occurred when lecithin was depleted from neurospora membranes by choline starvation. | 1977 | 140164 |
| inhibition of germ tube formation in neurospora. | phenethyl alcohol, m-cresol, and related compounds cause inhibition of germ tube formation in conidia of neurospora crassa. conidia continue to swell and form large spherical cells that are capable of multiple germ tube formation upon removal of inhibitor. | 1977 | 67112 |
| a temperature-sensitive mutant of neurospora crassa deficient in cytochrome b. | a nuclear gene mutant of neurospora crassa designated cyb-3 is deficient in cytochrome b and coenzyme qh2-cytochrome c reductase. nearly normal when grown at 25 degrees c, the strain expresses a mutant phenotype at 38 degrees c. mitochondria from cyb-3 mycelium, which has undergone 3-4 mass doublings at the elevated temperature, possess 3-fold less cytochrome b, 2-fold more cytochrome, c, 5-fold less coenzyme qh2-cytochrome c reductase activity, and require 3-fold less antimycin a per milligram ... | 1977 | 141003 |
| inhibition of aminoacyl-transfer ribonucleic acid synthetases and the regulation of amino acid biosynthetic enzymes in neurospora crassa. | growth conditions that result in the accumulation of the tryptophan intermediate indoleglycerol phosphate or of the histidine intermediate imidazoleglycerol phosphate cause mycelia of neurospora crassa to exhibit an immediate and sustained increase in the differential rate at which the biosynthetic enzymes of the tryptophan, histidine, and arginine pathways are synthesized. these accumulated intermediates are shown to be inhibitors of the activity of aminoacyltransfer ribonucleic acid (trna) syn ... | 1977 | 191433 |
| the subunit structure of the arom multienzyme complex of neurospora crassa. a possible pentafunctional polypeptide chain. | a new procedure for the purification of the arom multienzyme complex from neurospora crassa is presented. important factors are the inactivation of proteinases by phenylmethanesulphonyl fluoride and the use of cellulose phosphate as an affinity adsorbent. a homogeneous enzyme, with a specific shikimate dehydrogenase activity of 70 units/mg of protein, is obtained in 25% yield. polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate, combined with cross-linking studies using ... | 1977 | 139889 |
| genetic and kinetic analysis of neurospora crassa mtr mutants. | allelic complementation occurs at the mtr (methyltryptophan resistance) locus. kinetic properties of neutral amino acid transport are altered for mtr mutants. | 1977 | 139402 |
| control of the production of orthophosphate repressible extracellular enzymes in neurospora crassa. | the abilities of purine- and pyrimidine-requiring mutants to produce six orthophosphate repressible extracellular enzymes, alkaline phosphatase, 5'-nucleotidase, acid phosphatase, two nucleases and ribonuclease n1 were examined by culturing these mutants in low and high phosphate media containing nucleotide or nucleoside. all the purine requiring mutants produced significantly reduced amounts of alkaline phosphatase, 5'-nucleotidase, acid phosphatase, alkaline nuclease and acid nuclease ranging ... | 1977 | 194139 |
| characterization of the new osmotic mutants (os) which originated during genetic transformation in neurospora crassa. | 1977 | 141392 | |
| reactions of the neurospora crassa nitrate reductase with nad(p) analogs. | the assimilatory nadph-nitrate reductase (nadph:nitrate oxidoreductase, ec 1.6.6.3) from neurospora crassa is competitively inhibited by 3-aminopyridine adenine dinucleotide (aad) and 3-aminopyridine adenine dinucleotide phosphate (aadp) which are structural analogs of nad and nadp, respectively. the amino group of the pyridine ring of aad(p) can react with nitrous acid to yield the diazonium derivative which may covalently bind at the nad(p) site. as a result of covalent attachment, diazotized ... | 1977 | 12830 |
| light-dependent carotenoid synthesis : x. lag-phase after a second illumination period in fusarium aquaeductuum and neurospora crassa. | in dark grown mycelia of the fungi fusarium aquaeductuum lagh. and neurospora crassa the kinetics of carotenoid accumulation after brief illuminations were investigated. following a second illumination period photoinduced carotenoid biosynthesis started also only after a lag-phase. such a "secondary" lag-phase not only appeared when the pigment accumulation induced by the first light treatment had been completed, but also when the second illumination was given during the period of rapid caroteno ... | 1977 | 24420226 |
| dna syntheses in course of meiotic development in neurospora crassa. | 1977 | 141391 | |
| the effect of various "magdala reds" in inducing paramorphic colony growth in neurospora crassa. | 1977 | 139210 | |
| morphology of slime variants of neurospora crassa growing on a glass surface in liquid medium. 1. under normal conditions and 2. in the presence of inhibitors. | 1977 | 145837 | |
| an inducible acetate transport system in neurospora crassa conidia. | neurospora crassa conidia possess an active transport system for the uptake of acetate. this system was characterized as: (a) energy dependent; (b) taking place against a concentration gradient; (c) saturating at higher substrate concentrations and (d) competitively inhibited by propionate. activity of the acetate transport system can be further enhanced by preincubating conidia in 1 mm acetate medium for 180 min (the inducible transport system). the conidial system and the inducible system have ... | 1977 | 144526 |
| unstable s-adenosylmethionine synthetase in an ethionine-resistant strain of neurospora crassa. | a pleitropic ethionine-resistant mutant of neurospora contains an s-adenosylmethionine synthetase that is labile to heat and dialysis but exhibits normal kinetics for methionine and ethionine. | 1977 | 144115 |
| adaptive changes in phosphate uptake by the fungus neurospora crassa in response to phosphate supply. | the phosphate uptake rate of neurospora crassa germlings growing exponentially in media containing phosphate at concentrations between 10 mm and 50 micronm was virtually constant. the uptake characteristics of these germlings were studied in detail assuming the simultaneous operation of two uptake systems, one of low affinity and one of high affinity. the km of the low-affinity system was constant after growth at phosphate concentrations greater than 1 mm but became progressively lower as the co ... | 1977 | 144114 |
| cyclic amp and the plasma membrane potential in neurospora crassa. | diverse treatments, which have been shown by slayman, c. l. (1977) in water relations in membrane transport in plants and animals (jungreis, a., hodges, t. k., kleinzeller, a., and schultz, s. g., eds) pp. 69-86, academic press, new york, to depolarize the plasma membrane of neurospora, increase levels of adenosine 3':5'-monophosphate (cyclic amp) in the organism. the treatments include those producing large transport fluxes of metabolizable or nonmetabolizable compounds, rapid temperature drops ... | 1977 | 198403 |
| mitochondrial ribosome assembly in neurospora crassa. chloramphenicol inhibits the maturation of small ribosomal subunits. | 1977 | 146091 | |
| isolation and preparation of pretyrosine, accumulated as a dead-end metabolite by neurospora crassa. | pretyrosine is an amino acid intermediate of phenylalanine and/or tyrosine biosyntheses in a variety of organisms. a procedure for the isolation of high-quality pretyrosine as the barium salt is described. stable solutions of ammonium pretyrosine that are suitable for use as substrate in enzyme assays can be prepared in good yield with relatively few purification steps. a triple mutant of neurospora crassa, bearing genetic blocks corresponding to each initial enzyme step of the three pathway bra ... | 1977 | 144721 |
| germination-defective mutant of neurospora crassa that responds to siderophores. | a conditionally germination-defective mutant of neurospora crassa has been found to be partially curable by ferricrocin and other siderophores. the mutant conidia rapidly lose their membrane-bound siderophores when suspended in buffer or growth media. germination is consequently delayed unless large numbers of conidia are present (positive population effect). this indicates that the mutant has a membrane defect involving the siderophore attachment site. | 1977 | 144720 |
| nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of neurospora crassa. ix. isolation and sequences of several large cyanogen bromide peptides. | the isolation and sequences of several large peptides from cyanogen bromide cleavage of the 1030-residue polypeptide chain of the nad-specific glutamate dehydrogenase of neurospora crassa are described. one of these is in the 669-residue sequence of the cooh-terminal end of the protein. the remaining peptides have been aligned in two partial sequences in the nh2-terminal portion of the polypeptide chain. | 1977 | 199604 |
| nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of neurospora. viii. isolation and sequences of peptides from a tryptic hydrolysate of the maleylated protein. | the nad-specific glutamate dehydrogenase of neurospora crassa was s-carboxymethylated with [14c]iodoacetate, maleylated, and hydrolyzed with trypsin. the isolation and sequences of the resulting peptides are described. these peptides gave information on the structure of the protein that was previously unknown and gave many overlaps of previously isolated segments of the protein. | 1977 | 199603 |
| nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of neurospora. vii. isolation and sequences of three large cyanogen bromide peptides. | the isolation and sequences of three peptides of large size from a cyanogen bromide digest of the nad-specific glutamate dehydrogenase of neurospora crassa are reported. these three peptides comprise 86, 117, and 134 residues, respectively, and represent approximately 30% of the estimated 1030 residues in the peptide chain. | 1977 | 199602 |
| regulation of glutamine synthetase in fed-batch cultures of neurospora crassa. | 1977 | 21661 | |
| the 73 s ribosome of neurospora crassa is the native mitochondrial ribosome. | 1977 | 143964 | |
| deficiency of subunit two of cytochrome oxidase in the mi-3 cytoplasmic mutant of neurospora crassa. | 1977 | 72665 | |
| kinetic characterization of the two phosphate uptake systems in the fungus neurospora crassa. | the kinetics of phosphate uptake by exponentially growing neurospora crassa were studied to determine the nature of the differences in uptake activity associated with growth at different external phosphate concentrations. conidia, grown in liquid medium containing either 10 mm or 50 micronm phosphate, were harvested, and their phosphate uptake ability was measured. initial experiments, where uptake was examined over a narrow concentration range near that of the growth medium, indicated the prese ... | 1977 | 144113 |
| the effect of phosphatidylcholine depletion on biochemical and physical properties of a neurospora crassa membrane mutant. | by using the choline starvation process it is possible to deplete the membranes of neurospora crassa choline auxotroph chol-1 of phosphatidylcholine, without affecting the viability of germinated spores or whole mycelium. spin label probes were used to examine the possible dependence of the physical state of cellular lipids on the presence of phosphatidylcholine in the membranes. increased freedom of rotational motion of lipid soluble probes was regularly detected in choline-starved mycelium. th ... | 1977 | 197995 |
| preparation of polypeptide subunits of cytochrome oxidase from neurospora crassa. | cytochrome oxidase was purified from neurospora crassa by ammonium sulfate fractionation in the presence of bile salts. the enzyme preparations contained 10-13 nmol of heme a per mg of protein; no other hemoproteins could be detected. dodecylsulfate gel electrophoresis resolved the enzyme complex into seven major bands, representing seven polypeptide subunits. a procedure is described that allows the isolation of these enzyme subunits on a large scale starting from a single batch of oxidase prep ... | 1977 | 199436 |
| role of lipids in the neurospora crassa membrane. ii. membrane potential and resistance studies; the effect of altered fatty acid composition on the electrical properties of the cell membrane. | the effect of doubling the saturated fatty acid content on the electrophysiology of neurospora crassa membranes was studied. intracellular membrane input resistance (rm) and potential (em) were measured for wild-type (w/t) and cel- (tween 40) organisms as a function of temperature. over the 0 to 40 degrees c temperature range studied, mean em values of both w/t and cel- (tw 40) organisms increased from -160 to -210 mv. this difference is greater than that expected from nernst potential considera ... | 1977 | 143534 |
| proof of de novo synthesis of the qa enzymes of neurospora crassa during induction. | in neurospora crassa three inducible enzymes are necessary to catabolize quinic acid to protocatechuic acid. the three genes encoding these enzymes are tightly linked on chromosome vii near methionine-7 (me-7). this qa cluster includes a fourth gene, qa-1, which encodes a regulatory protein apparently exerting positive control over transcription of the other three qa genes. however, an alternative hypothesis is that the qa-1 protein simply activates preformed polypeptides derived from the three ... | 1977 | 144915 |
| uptake and efflux of adenine and its derivatives in neurospora crassa. | conidia of wild-type neurospora crassa, preincubated for 3 1/2 h in growth medium, showed a typical triphasic pattern of adenine uptake. the three phases consisted of a quick initial uptake, followed by a plateau phase, and then by a resume lowered uptake. a study of the relative influx and efflux of [14c] adenine showed that the plateau phase in fact is a period of transmembrane movement of adenine and adenine metabolites. the efflux during the plateau phase essentially cancelled out all the in ... | 1977 | 144552 |
| unsaturated fatty acid mutants of neurospora crassa. | unsaturated fatty acid (ufa) auxotrophs of neurospora crassa were obtained by treatment of conidia with n-methyl-n'-nitro-n-nitrosoguanidine followed by isolation on media containing polyunsaturated fatty acids suspended in tergitol np-40. the 24 mutants for which reisolates were obtained from crosses with wild type were assigned to two complementation classes, ufa-1 and ufa-2, located on linkage group v. unsaturated fatty acids with varying degrees of unsaturation, chain length, and double-bond ... | 1977 | 140860 |
| nitrogen regulation of arginase in neurospora crassa. | the final products of the arginine catabolism that can be utilized as a nitrogen source in neurospora crassa are ammonium, glutamic acid, and glutamine. the effect of these compounds on arginase induction by arginine was studied. in wild-type strain 74-a, induction by arginine was almost completely repressed by glutamic acid plus ammonium, whereas ammonium or glutamic acid alone had only moderate effects. arginine products of catabolism also repressed arginase induction. a mutant, ure-1, which l ... | 1977 | 142762 |
| nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of neurospora. vi. isolation and sequences of eighteen fragments from the cyanogen bromide digest. | the 1030-residue polypeptide chain of the nad-specific glutamate dehydrogenase of neurospora crassa was fragmented by treatment with cyanogen bromide. the isolation and sequences of 18 fragments ranging in size from 4 to 51 residues are described. some of these peptides proved to be cleavage products resulting from hydrolysis at acid-sensitive aspartyl-prolyl bonds. some overlaps could be deduced on the basis of known sequences of peptides obtained by tryptic hydrolysis. | 1977 | 199601 |
| nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of neurospora. v. tryptic peptides. | the isolation and sequences of an additional 80 peptides from a tryptic digest of the nad-specific glutamate dehydrogenase of neurospora crassa are reported. these include an additional peptide containing a lysine residue labeled at the epsilon-amino group with pyridoxal 5'-phosphate. the sequence of this peptide shows some homology with the reactive lysine residue of other glutamate dehydrogenases. | 1977 | 144134 |
| the stereospecificity of nitrate reductase for hydrogen removal from reduced pyridine nucleotides. | the stereospecificity of the hydrogen removal from reduced pyridine nucleotides catalyzed by nitrate reductase (nadh : nitrate oxidoreductase, ec 1.6.6.1, and nad(p)h : nitrate oxidoreductase, ec 1.6.6.2) was investigated. a high degree of enzyme purification was required to obtain conclusive results. improvements are described for the purification of nitrate reductase from chlorella fusca and from spinach (spinacea oleracea, l.) leaves. the latter enzyme is shown to contain a cytochrome. with h ... | 1977 | 16653 |
| isocitrate lyase from neurospora crassa: ph dependence of catalysis and interaction with substrates and inhibitors. | 1977 | 18092 | |
| metabolism of mandelic acid by neurospora crassa. | preliminary studies on the metabolism of manelic acid by neurospora crassa reveal the operation of a pathway for its degradation which involves benzoyl formic acid, benzaldehyde, benzoic acid, 4-hydroxybenzoic acid, and protocatechuic acid as the intermediates. this pathway is different from the followed by bacterial systems and is the same as that observed in aspergillus niger. | 1977 | 21739 |
| a complete cleavage map of neurospora crassa mtdna obtained with endonucleases eco ri and bam hi. | a physical map of neurospora crassa mitochondrial dna has been constructed using specific fragments obtained with restriction endonucleases. the dna has 5 cleavage sites for endonuclease bam hi, 12 for endonuclease eco ri and more than 30 for endonuclease hind iii. the sequence of the eco ri and bam hi fragments has been established by analysis of partial fragments. by digestion of the eco ri fragments with bam hi, a complete overlapping map has been constructed. the position of the largest hind ... | 1977 | 139928 |
| the ribosomal rna genes on neurospora crassa mitochondrial dna are adjacent. | hybridization of separated 24 s and 17 s ribosomal rna from neurospora crassa mitochondrial ribosomes to restriction fragments of mitochondrial dna leads to the conclusion that the large and small ribosomal rna are adjacent on the restriction endonuclease cleavage map of the dna. the distance between the two genes is estimated at 900 basepairs. this result is consistent with the existence of a ribosomal precursor rna in n. crassa mitochondria and is in contrast to the situation in yeast, where t ... | 1977 | 143295 |
| enzymatic release of iron from sideramines in fungi. nadh:sideramine oxidoreductase in neurospora crassa. | young mycelia of the fungus neurospora crassa contain a soluble nadh-linked sideramine reductase, which may be responsible for liberating iron in vivo from accumulated sideramines during iron-deficient cultivation. the enzymes can be assayed using a soluble supernatant fraction, edta, and an atmosphere of pure nitrogen. the enzyme is stable without loss of activity up to 45 degrees c and has an optimum of activity at ph 7.0. besides coprogen (km = 100 micrometer, v=2.8 nmol/min per mg protein), ... | 1977 | 144535 |
| molybdenum cofactors from molybdoenzymes and in vitro reconstitution of nitrogenase and nitrate reductase. | a molybdenum cofactor (mo-co) from xanthine oxidase (xanthine:oxygen oxidoreductase, ec 1.2.3.2) can be isolated from the enzyme by a technique that has been used to isolate an iron-molybdenum cofactor (femo-co) from component i of nitrogenase. n-methylformamide is used for the extraction of these molybdenum cofactors. mo-co from xanthine oxidase activates nitrate reductase (nadph:nitrate oxidoreductase, ec 1.6.6.2) in an extract from neurospora crassa mutant strain nit-1; however, femo-co is un ... | 1977 | 146198 |
| biosynthesis of carnitine in neurospora crassa. | in growing cultures of neurospora crassa lysine auxotroph 33933, (a) beta-hydroxy-epsilon-n-trimethyllysine and gamma-n-trimethylaminobutyraldehyde, postulated precursors of carnitine in the rat, effectively blocked synthesis of labeles carnitine from epsilon-n-[ch3-3h]trimethyllysine; and (b) beta-hydroxy-epsilon-n[ch3-3h[trimethyllysine and gamma-n-[ch3-3h]trimethylaminobutyraldehyde were effectively utilized for carnitine formation. from these isotopic experiments, the latter steps of carniti ... | 1977 | 144128 |
| expression in escherichia coli k-12 of the structural gene for catabolic dehydroquinase of neurospora crassa. | the inducible quinic acid catabolic pathway of neurospora crassa is controlled by four genes, the qa cluster which includes structural genes qa-2, qa-3, qa-4 for three enzymes and a regulatory gene, qa-1. in this paper we report the molecular cloning of at least the qa-2 gene which encodes the catabolic dehydroquinase (5-dehydroquinate hydro-lyase, ec 4.2.1.10). endo.r.hindiii restriction endonuclease fragments of n. crassa dna from a qa-1(c) (constitutive) mutant and of escherichia coli plasmid ... | 1977 | 143663 |
| expression of the structural gene for catabolic dehydroquinase of neurospora crassa in escherichia coli k12. | 1977 | 157795 | |
| regulation of exocellular protease in neurospora crassa: induction and repression under conditions of nitrogen starvation. | 1977 | 143241 | |
| neurospora crassa glutamine synthetase. translation of specific messenger ribonucleic acid in a cell-free system derived from rabbit reticulocytes. | the total reticulocyte lysate cell-free protein-synthesizing system was incubated in the presence of neurospora crassa rna. with the aid of an antibody directed against purified n. crassa glutamine synthetase, the synthesis of a specific protein was detected. this protein precipitates with antiglutamine synthetase using both direct and indirect procedures, migrates with the same molecular weight as the monomer of n. crassa glutamine synthetase when subjected to acrylamide gel electrophoresis in ... | 1977 | 16013 |
| mutation induction by the antischistosomal drug f30066 in various test systems. | the genetic activity of furapromidium (f30066), an antischistosomal drug, was studied in salmonella typhimurium, saccharomyces cerevisiae, neurospora crassa and cultured chinese hamster cells. the results show that f30066 induces gene mutations in s. typhimurium, n. crassa and chinese hamster cells. this compound also causes gene conversions in s. cerevisiae. | 1977 | 138086 |
| nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of neurospora. iv. the cooh-terminal 669 residues of the peptide chain; comparison with other glutamate dehydrogenases. | a sequence is presented for the cooh-terminal 669 residues of the nad-specific glutamate dehydrogenase of neurospora crassa. comparison of this sequence with those of the vertebrate glutamate dehydrogenases of chicken and bovine liver and with the nadp-specific enzyme of neurospora shows some similarities in sequences around residues previously identified as important for the function of these enzymes. these are: (a) the reactive lysine residue of low pk in the nadp and the vertebrate enzymes; ( ... | 1977 | 21191 |
| nucleotide sequence of neurospora crassa cytoplasmic initiator trna. | initiator methionine trna from the cytoplasm of neurospora crassa has been purified and sequenced. the sequence is: pagcugcaum1ggcgcagcggaagcgcm22gcy*gggcucaut6aacccggagm7gu (or d) - cacucgaucgm1aaacgag*uugcagcuaccaoh. similar to initiator trnas from the cytoplasm of other eukaryotes, this trna also contains the sequence -aucg- instead of the usual -tphicg (or a)- found in loop iv of other trnas. the sequence of the n. crassa cytoplasmic initiator trna is quite different from that of the corresp ... | 1977 | 146192 |
| in situ effects of s1 endonuclease (neurospora crassa) on pachytene chromatin of xenopus laevis females at metamorphosis. | 1977 | 837986 | |
| determination of the absolute configuration at c-20 and c-24 of ergosterol in ascomycetes and basidiomycetes by proton magnetic resonance spectroscopy. | samples of ergosterol isolated from saccharomyces cerevisiae, neurospora crassa, and agaricus sp., and commercial ergosterol all displayed identical proton magnetic resonance (pmr) spectra at 220 mhz. from the effects produced on the doublet for c-21 by epimerization at c-20 and c-24 in sterols of known configuration, the absolute configurations at these positions in ergosterol were determined. the data demonstrate that ergosterol from both ascomycetes and basidiomycetes is the same and that at ... | 1977 | 853879 |
| differential excision from dna of the c-8 and n2 guanosine adducts of n-acetyl-2-aminofluorene by single strand-specific endonucleases. | purified duck reticulocyte dna was reacted in vitro with [9-14c]-n-acetoxy-n-acetyl-2-aminofluorene. hydrolysis of the [14c]-n-acetyl-2-aminofluorene-modified dna followed by sephadex lh-20 column chromatography showed that 85% of the dna-bound [14c]-n-acetyl-2-aminofluorene was n-(deoxyguanosin-8-yl)-n-acetyl-2-aminofluorene and 15% was 3-(deoxyguanosin-n2-yl)-n-acetyl-2-aminofluorene. when this modified dna was incubated with the single strand-specific nuclease, s1, and the undigested fraction ... | 1977 | 908018 |
| cytochrome c-specific protein methylase iii from neurospora crassa. | a protein methylase iii responsible for specifically methylating the cytochrome c in neurospora crassa was partially characterized by using unmethylated horse heart cytochrome c as a substrate. this enzyme utilizes s-adenosyl-l-methionine as the methyl donor. an analysis of the distribution of [14c]methyl groups in the peptides obtained by chymotrypsin digestion of the enzymically methylated cytochrome c showed that all of the radioactivity could be recovered within a single peak after chromatog ... | 1977 | 196592 |
| [effect of liver chromatin dnase on closed circular dna of pm-2 phage and simian virus 40]. | comparative effect of the dnase from rat liver chromatin and neurospora crassa endonuclease s1 on closed circular superhelical dna of pm-2 phage and simian virus 40 is studied. it is shown that both of them--the dnase from chromatin proteins and endonuclease s1--are specific to single-stranded regions in dna molecular. it is suggested that chromatin protein dnase participates in reparation processes. | 1977 | 196682 |
| introduction. membrane transport of peptides. | carrier-mediated membrane transport of small peptides is now realized to be a process of wide biological distribution, occurring not only in the small intestine and elsewhere in the animal body but also in bacteria, yeast, the mould neurospora crassa, and probably in higher plants during the germination of seeds. the important features of peptide transport are outlined, and possible relationships between peptide transport and hydrolysis are discussed. peptide transport is a stereochemically spec ... | 1977 | 244390 |
| studies on the transcription complex of escherichia coli rna polymerase. | to study the chain elongation phase of enzymatic rna synthesis ternary transcription complexes with t7 dna or poly[da) - (dt)] as template were isolated by gel exclusion chromatography. the dna in these complexes contains single-stranded regions which are recognized by a single-strand-specific nuclease from neurospora crassa. the non-codogenic dna strand in the poly[(da) - (dt)] ternary complex is preferentially hydrolysed by the nuclease. the polymerase protects predominantly the codogenic stra ... | 1977 | 336358 |
| isolation of precursors of cytochrome oxidase from neurospora crassa: application of subunit-specific antibodies and protein a from staphylococcus aureus. | a novel immunological procedure has been applied for the isolation of precursors of cytochrome oxidase. it involves antibodies to individual subunits of the oxidase and protein a from staphylococcus aureus linked to a sepharose support. unassembled (free) 'subunits' as well as a labile complex containing five polypeptide components of the oxidase were isolated from mitochondrial extracts by this technique. the procedure is superior to the previously used double-immuno-precipitation method, becau ... | 1978 | 213273 |
| structural analysis of viral replicative intermediates isolated from adenovirus type 2-infected hela cell nuclei. | deoxyribonucleoprotein complexes released 17 h postinfection from adenovirus type 1 (ad2)-infected hela cell nuclei were shown by electron microscopy to contain filaments much thicker (about 200 a [20 nm]) than double-stranded dna (about 20 a [2 nm]). the complexes were partially purified through a linear sucrose gradient, concentrated, and further purified in a metrizamide gradient. the major protein present in the complexes was identified as the 72,000-dalton (72k), adenovirus-coded single-str ... | 1978 | 207893 |
| fungal transformation of naphthalene. | eighty-six species of fungi belonging to sixty-four genera were examined for their ability to metabolize naphthalene. analysis by thin-layer and high pressure liquid chromatography revealed that naphthalene metabolism occurred in forty-seven species belonging to thirty-four genera from the major fungal taxa. all organisms tested from the order mucorales oxidized naphthalene with species of cunninghamella, syncephalastrum and mucor showing the greatest activity. significant metabolism was also ob ... | 1978 | 678019 |
| isolation and characterization of isocitrate lyase from a thermophilic bacillus sp. | isocitrate lyase was isolated in homogeneous state from a thermophilic bacillus. the enzyme has a mol.wt. of 180000 and a pi of 4.5 and contains threonine as the n-terminal residue. it resembles in size the cognate enzyme from the mesophilic bacterium pseudomonas indigofera, but is smaller than the enzyme from the eukaryotic fungus neurospora crassa. all three lyases are tetramers and similar in amino acid composition, but the thermophile enzyme is distinctive from its mesophilic coutnerparts in ... | 1978 | 687365 |
| mutagenicity and mutagenic specificity of metronidazole and niridazole in neurospora crassa. | mutagenicity and mutagenic specificity of niridazole and metronidazole, two chemotherapeutic agents used in the treatment of human parasitic diseases, were studied with the ad-3 test system of neurospora crassa. the results show that neither compound is mutagenic in resting conidia. in growing vegetative cells, however, both compounds are mutagenic in n. crassa. genetic analysis of the mutants indicated that niridazole induces predominantly base-pair substitution mutations. none of the niridazol ... | 1978 | 153406 |
| isolation of a multiprotein complex containing cytochrome b and c1 from neurospora crassa mitochondria by affinity chromatography on immobilized cytochrome c. difference in the binding between ferricytochrome c and ferrocytochrome c to the multiprotein complex. | a multiprotein complex which contains in equimolar amounts two cytochromes b (mr each about 27,000), one cytochrome c1 (mr 31,000) and six subunits without known prosthetic groups (mr 8000, 12,000, 14,000, 45,000, 45,000, and 50,000) has been isolated from the mitochondrial membranes of neurospora crassa by affinity chromatography on immobilized cytochrome c. the chromatographic separation was based upon the specific binding of the complex to ferricytochrome c coupled to sepharose and its specif ... | 1978 | 27360 |
| use of the spot, plate and suspension test systems for the detection of the mutagenicity of environmental agents and chemical carcinogens in neurospora crassa. | n23 and n24, selected from hundreds of ad-3 mutants, have been used as testers for the spot, plate and suspension tests in neurospora crassa. these two testers are highly sensitive to mutagens and are revertible by a specific group of chemicals. n23 can be reverted from an adenine-dependence to adenine-independence by agents which cause base-pair substitutions whereas n24 can be reverted by frameshift mutagens. studies described here show that spot, plate and suspension tests using testers n23 a ... | 1978 | 149924 |
| presence of an actin-like protein in mycelium of neurospora crassa. | addition of atp, cacl2, and kcl to supernatants prepared from mycelia of snowflake (strain 507), a morphological mutant of neurospora crassa, results in the formation of filaments 70 nm in diameter. the "decorated" appearance of these filaments after incubation with heavy meromyosin from rabbits suggests they are actin-like. | 1978 | 150412 |
| transcription and translation in e. coli of hybrid plasmids containing the catabolic dehydroquinase gene from neurospora crassa. | two hybrid plasmids which carry the gene for neurospora crassa catabolic dehydroquinase (c-dhqase) and complement an arod6 (dehydroquinase-deficient) auxotroph of escherichia coli have been analyzed. one of these contains a 2.9 kilobase (kb) fragment cloned in the hindiii site of plasmid pbr322 (pvk57) and the other contains a 6.8 kb fragment cloned in the psti site (pvk88). restriction enzyme mapping of these plasmids has demonstrated that the 2.9 kb fragment is totally contained within the 6.8 ... | 1978 | 154430 |
| isolation of neurospora crassa-bovine l-glutamate dehydrogenase hybrids [proceedings]. | 1978 | 154423 | |
| organization of the ribosomal ribonucleic acid gene cluster of neurospora crassa by means of restriction endonucleases and cloning in bacteriophage lambda [proceedings]. | 1978 | 154424 | |
| neurospora crassa mitochondrial transfer rnas. | total mitochondrial trna from neurospora crassa was characterized by base composition analysis, one- and two-dimensional gel electrophoreses and reversed-phase chromatography on rpc5. the guanosine + cytidine content was about 43%, as compared to 60% for cytoplasmic trna. the modified nucleoside content was low and about the same as that of total yeast mitochondrial trna, though the g + c content is very different. we found psi, t, hu, t6a, m1g, m2g, m22g. neither the eukaryotic "y" base, nor th ... | 1978 | 152130 |
| nitrogen source regulates glutamine synthetase mrna levels in neurospora crassa. | neurospora crassa glutamine synthetase mrna was measured by its capacity to direct the synthesis of the specific protein in a cell-free system derived from rabbit reticulocytes. n. crassa cultures grown on glutamate as the sole nitrogen source had higher mrna activities than did those grown on glutamine. the differences were about 10-fold when polysomal rna was used for translation and about 5-fold when either total cellular rna or polyadenylic acid-enriched cellular rna was used. these data ind ... | 1978 | 31352 |
| the enzymic synthesis of beta-[32p]udp-n-acetylglucosamine. | cells of micrococcus sp. 2102 incorporate inorganic [32p]phosphate from the medium into the sugar-phosphate polymer of the wall. controlled acid hydrolysis of sodium dodecyl sulphate-extracted cells gives n-acetylglucosamine 6-[32p]-phosphate which can be purified by ion-exchange chromatography and incubated with utp in the presence of crude preparations of phosphoacetylglucosamine mutase from neurospora crassa and utp:n-acetylglucosamine 1-phosphate phosphotransferase from bacillus licheniformi ... | 1978 | 9762094 |
| isolation and characterization of perithecial development mutants in neurospora. | the isolation and characterization of mutants that block perithecial development in neurospora crassa are described. several classes of mutants have been isolated after uv mutagenesis, and those that block perithecial development when used as the female (protoperithecial) component of a cross have been further characterized. these mutants fall into 29 complementation groups. twelve of the 33 mutants block development at the protoperithecial stage; no other clustering of block points is observed. ... | 1978 | 17248793 |
| a comparative study of dna-induced transformants and spontaneous revertants of inositolless neurospora crassa. | inositolless (inl-) neurospora crassa strains were treated with dna (allo-dna) of wild type n. crassa. hyphal fragments of a mycelial suspension of the n. crassa ragged inl- strain used as recipient in our transformation experiments were found to consist of units containing 100--1000 nuclei. in this strain the inositol-independent (inl+) nuclei appearing after dna treatment or by spontaneous reversion are present in the cytoplasm together with a large number of inl- nuclei. thus, both transforma ... | 1978 | 161130 |
| physiological response of neurospora conidia to freezing in the dehydrated, hydrated, or germinated state. | this study concerned the response to freezing of neurospora crassa conidia in four different states: air-dry, hydrated in water, hydrated in vogel medium lacking only sucrose, or hydrated in complete vogel medium. all hydrated conidia were incubated in one of the above media for various times before freezing and were then washed and frozen in distilled water. viability was estimated by three techniques, and the agreement among them was good. hydration of air-dry conidia was found to be very rapi ... | 1978 | 146455 |
| structure and function of initiator methionine trna from the mitochondria of neurospora crassa. | 1978 | 145896 | |
| association and dissociation of neurospora crassa cytoplasmic ribosomes. | dissociation and association factors of ribosomal particles were detected in extracts from neurospora crassa at different stages of growth. the dissociation factor was easily released into the s100 supernatant fraction, whereas the association factor remained bound to the ribosomes. | 1978 | 145436 |
| factors influencing the uptake of dna by neurospora crassa. | under optimal conditions intact neurospora crassa cells incorporated nearly the same amount of 3h-labelled dna as that of the endogenous dna content of cells. after 18 h of incorporation more than 80 per cent of the radioactivity was retained in the cells. a maximum uptake of exogenous dna occurred at 28 degrees c, ph 6.35, in the presence of 100 mm calcium when the concentration of donor dna was 150 micrograms/ml. denatured dna was incorporated at a higher rate than native dna. the present find ... | 1978 | 39418 |
| kinetic properties of pyruvate kinase of neurospora crassa at physiological ph. | the kinetic properties of pyruvate kinase (ec 2.7.1.40) extracted from the mycelia of neurospora crassa were examined at physiological ph to determine the role of the enzyme in the regulation of glycolysis. the velocity curve with the substrates, phosphoenolpyruvate and adenosine diphosphate, are hyperbolic. the effect of magnesium, potassium, or calcium on the enzyme is influenced by the ph but not to the extent that would change their role as cofactor or inhibitor. adenosine triphosphate and c ... | 1978 | 27701 |
| subunit ratios of separated hybrid hexamers of neurospora nadp-specific glutamate dehydrogenase containing complementing mutationally modified monomers. | the am1 and am3 mutational variants of the neurospora crassa nadp-specific glutamate dehydrogenase show complementation activity in hybrid hexamers. a freeze-thaw hybridization method was used to construct hybrids from purified enzymes and the products were separated into species of different monomer ratio by affinity chromatography. hexamers with am1:am3 ratios of 1:5, 2:4, 3:3, 4:2 and 5:1 were all recovered as resolved or partially resolved peaks in quantities approximating to a binomial dist ... | 1978 | 33665 |
| cyclic amp and cyclic gmp in germinating conidia of neurospora crassa. | a new method for obtaining synchronous germination allowed accurate time-course studies of endogenous levels of cyclic adenosine 3',5'-monophosphate and cyclic guanosine 3',5'-phosphate in germinating conidia of neurospora crassa. the levels of both cyclic nucleotides remained constant throughout germination, showing that they neither signal nor respond to any of the biochemical changes which are known to occur in the germination process. conidal germination was approximately normal in three cr- ... | 1978 | 211974 |
| purification of cytochrome oxidase from neurospora crassa and other sources. | 1978 | 213692 | |
| disappearance of the cyanide-insensitive pathway of oxidation in mitochondria of mi-1 mutant of neurospora crassa in vitro. | oxidation of exogenous nadh in mitochondria isolated from wild type and mi-1 mutant of neurospora crassa decreases rapidly in vitro. in mi-1 mutant mitochondria the inactivation concerns the alternate pathway of oxidation whereas in the wild type it involves an unknown component of the respiratory chain. the activity of the primary nadh dehydrogenase is constant within the time of the experiments (2-4 h). nadh oxidase is not inactivated if oxygen is removed from the incubation medium by nitrogen ... | 1978 | 208334 |
| 5' cap methylation of homologous poly a(+) rna by a rna (guanine-7) methyltransferase from neurospora crassa. | 1978 | 151539 | |
| a meiotic uv-sensitive mutant that causes deletion of duplications in neurospora. | the meiotic-3 (mei-3) mutant of neurospora crassa has several effects: (1) when homozygous, it almost completely blocks meiosis and ascospore formation, (2) it is sensitive to uv, (3) its growth is inhibited by histidine and, (4) it increases the instability of nontandem duplications. this was shown for duplications produced by five different rearrangements and was demonstrated by two different criteria. the effects on meiosis and duplication instability are expressed strongly at 25 degrees ; th ... | 1978 | 17248837 |
| nuclear mutations conferring oligomycin resistance in neurospora crassa. | mutants of neurospora crassa have been isolated that are highly resistant to inhibition by oligomycin, an inhibitor of mitochondrial atpase activity. dixon plots (dixon, m., and webb, e.c. (1964) enzymes, 2nd ed, pp. 328-330, academic press, new york) of oligomycin inhibition curves of the parent strain and the resistant mutants are linear, indicating that oligomycin interacts at a single site within the atpase complex. the ki values obtained from the mutants vary from 150 to 900 times greater t ... | 1978 | 149126 |
| stability of messenger rna for nitrate reductase in neurospora crassa. | a method has been developed to study the synthesis and decay of the messenger rna for nitrate reductase in neurospora crassa. glutamine prevents the synthesis of the mrna which appears to have a half-life of approximately 8.5 min. | 1978 | 149559 |
| photosensitivity of respiration in neurospora mitochondria. a protective role for carotenoid. | 1. the effect of visible light on respiratory activity was studied in two strains of neurospora crassa, one a wild-type strain able to synthesize carotenoid and the other an albino mutant lacking carotenogenic activity. light had no effect on growth under the conditions studied, but inhibited respiration of hyphal suspensions. the degree of inhibition being dependent on the carotenoid content of the hyphae. 2. in studies of respiration of isolated mitochondria, three types of photosensitive site ... | 1978 | 154887 |
| an epr analysis of cyanide-resistant mitochondria isolated from the mutant poky strain of neurospora crassa. | an analysis of the paramagnetic components present in mitochondria isolated from the poky mutant of neurospora crassa is described. the study was undertaken with a view to shedding light on the nature of the cyanide- and antimycin a-resistant alternative terminal oxidase which is present in these preparations. of the ferredoxin-type iron-sulfure centers, only centers s-1 and s-2 of succinate dehydrogenase could be detected in significant quantities. paramagnetic centers attributable to site i we ... | 1978 | 214109 |
| isolation and characterization of glycerol-3-phosphate dehydrogenase-defective mutants of neurospora crassa. | three glycerol-nonutilizing mutants deficient in the mitochondrial glycerol-3-phosphate (g3p) dehydrogenase (ec 1.1.99.5) were isolated from inl(ts) derivatives of neurospora crassa following inositolless death at elevated temperatures on minimal glycerol medium. these mutants failed to grow on glycerol as a sole carbon source, but could grow on acetate, glucose, or mannitol media and were female fertile in genetic crosses, thereby distinguishing them from the previously reported polyol-protoper ... | 1978 | 152757 |
| specificity of nucleoside transport in neurospora crassa. | the specificity of nucleoside uptake in germinating conidia of neurospora crassa was investigated by examining the kinetics of [2-14c]uridine and [8-14c]-adenosine uptake in the wild-type, ad-8, and ud-1 pyr-1 strains. the results obtained strongly indicate that nucleoside transport in n. crassa is mediated solely by a general transport system which accepts both purine and pyrimidine nucleosides. studies directed at characterizing the specificity of the transport system indicate that general str ... | 1978 | 152756 |
| control of the activity of intracellular nucleases in neurospora crassa. | 1978 | 149909 |