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analysis of expressed sequence tags from the fungus aspergillus oryzae cultured under different conditions.we performed random sequencing of cdnas from nine biologically or industrially important cultures of the industrially valuable fungus aspergillus oryzae to obtain expressed sequence tags (ests). consequently, 21 446 raw ests were accumulated and subsequently assembled to 7589 non-redundant consensus sequences (contigs). among all contigs, 5491 (72.4%) were derived from only a particular culture. these included 4735 (62.4%) singletons, i.e. lone ests overlapping with no others. these data showed ...200717540709
improvement of the antitumor activity of black currant polysaccharide by an enzymatic treatment.a polysaccharide-rich substance isolated from black currant, named cassis polysaccharide (caps), was partially digested with beta-galactosidase from aspergillus oryzae and its immunostimulatory activity was investigated. the in vitro cytokine-inducing effect of caps on raw264 cells was gradually decreased along with lowering of the average mw of caps. in vivo, partially digested caps with a mean mw of approximately 20,000 showed the most potent antitumor activity against ehrlich carcinoma in mic ...200717485844
production of dye-decolorizing peroxidase (rdyp) from complex substrates by repeated-batch and fed-batch cultures of recombinant aspergillus oryzae.we examined production levels of dye-decolorizing peroxidase (rdyp) by recombinant aspergillus oryzae using wheat bran and rice bran powders in repeated-batch and fed-batch cultures. similar average rdyp productivities were observed in repeated-batch cultures using wheat bran powder and rice bran powder. average rdyp productivities in fed-batch cultures were slightly lower than those in repeated-batch cultures. the rdyp production was affected by the addition of k(2)hpo(4) in the repeated-batch ...200717368394
effects of fermented soybean meal on digestive enzyme activities and intestinal morphology in broilers.the experiment was performed to compare the effects of fermented soybean meal (fermented with aspergillus oryzae 3.042, fsbm) and soybean meal on digestive enzyme activities and intestinal morphology in broilers. three hundred twenty 1-d-old ross x ross male broiler chicks were randomly allocated into 2 dietary treatments for a 6-wk feeding trial, including 0- to 21-d and 21- to 42-d periods. at the end of each stage, 8 broilers of each treatment were killed, and pancreas, small intestine digest ...200717495085
enhancement of substrate recognition ability by combinatorial mutation of beta-glucosidase displayed on the yeast cell surface.recently, in family 3 beta-glucosidase (bgl), the catalytically important asp nucleophile has been identified in the sdw segment of the sdwg sequence by site-directed mutagenesis. however, the details about the roles of each amino acid residue of the sdwg sequence have not been investigated. w293 of the sdw segment, which is the residue next to the nucleophile (d292) in family 3 bgl, is very important for hydrolytic reaction as a binder to a substrate. g294 of the sdwg sequence might play an imp ...200717602218
[rapd analysis of aspergilli and its application in brewing industry].phylogenetic analysis of sixteen aspergilli was done by rapd technology, using aspergillus oryzae as3.951, aspergillus flavus gim3.18 and aspergillus sojae as3.495 as controls. first, genome dna of the sixteen test strains were prepared by improved extraction method, and their quality was verified by electrophoresis and spectrophotometry. they displayed an identical band (approximately 20 kb) in agarose gel electrophoresis, which conformed to the fact that these strains all belong to aspergillus ...200717672321
iron chelated cyclic peptide, ferrichrysin, for oral treatment of iron deficiency: solution properties and efficacy in anemic rats.ferrichrysin (fcy), which is produced by aspergillus oryzae and is present in foods used for human consumption, belongs to a group of hydroxamate siderophore ferric iron chelators. fcy (100 mg/ml) dissolves completely at both ph 2.0 and 7.0, being very stable at a wide range of ph, high temperatures and pressures, with little reactivity to dietary iron absorption inhibitors, phytic acid, tannic acid, and catechin. we studied the effect of fcy in male sprague-dawley rats with iron-deficiency anem ...200717685091
high performance microbiological transformation of l-tyrosine to l-dopa by yarrowia lipolytica nrrl-143.the 3,4-dihydroxy phenyl l-alanine (l-dopa) is a drug of choice for parkinson's disease, controlling changes in energy metabolism enzymes of the myocardium following neurogenic injury. aspergillus oryzae is commonly used for l-dopa production; however, potential improvements in ease of handling, growth rate and environmental impact have led to an interest in exploiting alternative yeasts. the two important elements required for l-dopa production are intracellular tyrosinases (thus pre-grown yeas ...200717705832
purification, characterization, and substrate specificity of a glucoamylase with steroidal saponin-rhamnosidase activity from curvularia lunata.it has been previously reported that a glucoamylase from curvularia lunata is able to hydrolyze the terminal 1,2-linked rhamnosyl residues of sugar chains at c-3 position of steroidal saponins. in this work, the enzyme was isolated and identified after isolation and purification by column chromatography including gel filtration and ion-exchange chromatography. analysis of protein fragments by maldi-tof/tof proteomics analyzer indicated the enzyme to be 1,4-alpha-d-glucan glucohydrolase ec 3.2.1. ...200717823796
development of an efficient production method for beta-mannosidase by the creation of an overexpression system in aspergillus aculeatus.to develop an overexpression system in aspergillus aculeatus in order to establish an efficient overproduction method of beta-mannosidase (manb).200717651209
marked production of ginsenosides rd, f2, rg3, and compound k by enzymatic method.the hydrolysis of protopanaxadiol-type saponin mixture by various glycoside hydrolases was examined. among these enzymes, crude preparations of lactase from aspergillus oryzae, beta-galactosidase from a. oryzae, and cellulase from trichoderma viride were found to produce ginsenoside f(2) [3-o-(beta-d-glucopyranosyl)-20-o-beta-d-glucopyranosyl-20(s)-protopanaxadiol], compound k [20-o-beta-d-glucopyranosyl-20(s)-protopanaxadiol], and ginsenoside rd {3-o-[beta-d-glucopyranosyl-(1-->2)-beta-d-glucop ...200717917300
absence of mutagenicity, genotoxicity, and subchronic oral toxicity of touchi extract.touchi, a traditional chinese food used mainly for seasoning is obtained by first steaming soybeans followed by fermentation with aspergillus oryzae (koji). a series of toxicological studies was conducted to evaluate the mutagenic and genotoxic potential and subchronic toxicity of a water extract of touchi, a known inhibitor of alpha -glucosidase activity. touchi extract (te) did not induce reverse mutations in salmonella typhimurium strains ta98, ta1537, ta100, ta1535, and escherichia coli wp2u ...200717963133
structure and characteristics of an endo-beta-1,4-glucanase, isolated from trametes hirsuta, with high degradation to crystalline cellulose.trametes hirsuta produced cellulose-degrading enzymes when it was grown in a cellulosic medium such as avicel or wheat bran. an endo-beta-1,4-glucanase (theg) was purified from the culture filtrate, and the gene and the cdna were isolated. the gene consisted of an open reading frame encoding 384 amino acids, interrupted by 11 introns. the whole sequence showed high homology with that of family 5 glycoside hydrolase. the properties of the recombinant enzyme (reg) in aspergillus oryzae were compar ...200717928699
development of high-throughput screening system by single-cell reaction using microchamber array chip.to demonstrate the practical use of a novel high-throughput screening system by a single cell reaction for the rapid screening of combinatorially mutated beta-glucosidase 1 from aspergillus oryzae, a yeast cell chip microchamber array in combination with yeast cell surface engineering was successfully developed.200717964493
reversible and irreversible unfolding of multi-domain proteins.in contrast to single-domain proteins unfolding of larger multi-domain proteins is often irreversible. in a comparative case study on three different multi-domain proteins (phosphoglycerate kinase: pgk and two homologous alpha-amylases: taka and bla) we investigated properties of unfolded states and their ability to fold back into the native state. for this purpose guanidine hydrochloride, alkaline ph, and thermal unfolded states were characterized. structural alterations upon unfolding and refo ...200717964867
identification and characterization of an intracellular lectin, calnexin, from aspergillus oryzae using n-glycan-conjugated beads.recently, asparagine-linked oligosaccharides (n-glycans) have been found to play a pivotal role in glycoprotein quality control in the endoplasmic reticulum (er). in order to screen proteins interacting with n-glycans, we developed affinity chromatography by conjugating synthetic n-glycans on sepharose beads. using the affinity beads with the dodecasaccharide glc(1)man(9)glcnac(2), one structure of the n-glycans, a 75-kda protein, was isolated from the membranous fraction including the er in asp ...200717986781
degradation of raffinose oligosaccharides in soymilk by immobilized alpha-galactosidase of aspergillus oryzae.alpha-galactosidase was immobilized in a mixture of k-carrageenan and locust bean gum. the properties of the free and immobilized enzyme were then determined. the optimum ph for both the soluble and immobilized enzyme was 4.8. the optimum temperature for the soluble enzymes was 50 degrees c, whereas that for the immobilized enzyme was 55 degrees c. the immobilized enzyme was used in batch, repeated batch, and continuous modes to degrade the raffinose-family sugars present in soymilk. two hours o ...200718062219
molecular cloning and characterization of an alpha-amylase from pichia burtonii 15-1.an alpha-amylase secreted by pichia burtonii 15-1 isolated from a traditional starter murcha of nepal, named pichia burtonii alpha-amylase (pba), was studied. the gene was cloned and its nucleotide sequence was determined. pba was deduced to consist of 494 amino acid residues. it shared certain degrees of amino acid sequence identity with other homologous proteins: 60% with schwanniomyces occidentalis alpha-amylase, 58% with saccharomycopsis sp. alpha-amylase, and 47% with taka-amylase a from as ...200718071262
some distinguishable properties between acid-stable and neutral types of alpha-amylases from acid-producing koji.the highly humid climate of japan facilitates the growth of various molds. among these molds, aspergillus oryzae is the most important and popular in japan, and has been used as yellow-koji in producing many traditional fermented beverages and foods, such as japanese sake, and soy sauce. taka-amylase a (taa), a major enzyme produced by the mold, is well known worldwide to be a leading enzyme for industrial utilization and academic study, since many extensive studies have been carried out with ta ...200718086434
aggregation of endosomal-vacuolar compartments in the aovps24-deleted strain in the filamentous fungus aspergillus oryzae.previously, we found that deletion of aovps24, an ortholog of saccharomyces cerevisiae vps24, that encodes an escrt (endosomal sorting complex required for transport)-iii component required for late endosomal function results in fragmented and aggregated vacuoles. although defective late endosomal function is likely responsible for this phenotype, critical lack of our knowledge on late endosomes in filamentous fungi prevented us from further characterization. in this study, we identified late en ...200717719006
new fermentation technique for complete digestion of soybean protein.the aim of this study was to develop a new fermentation method in order to improve the digestion of soybean protein, and to promote normal fermentation of soybean. a proximate composition, such as moisture, ph, and reducing sugar, of fermented soybeans by the new fermentation was similar to those of controls. neutral protease activity, the most important factor for fermented soybean products, was the highest, having about 636 u/g at 54 h fermentation. the content of total free amino acid was alm ...200718092480
identification and toxigenic potential of the industrially important fungi, aspergillus oryzae and aspergillus sojae.mold strains belonging to the species aspergillus oryzae and aspergillus sojae are highly valued as koji molds in the traditional preparation of fermented foods, such as miso, sake, and shoyu, and as protein production hosts in modern industrial processes. a. oryzae and a. sojae are relatives of the wild molds aspergillus flavus and aspergillus parasiticus. all four species are classified to the a. flavus group. strains of the a. flavus group are characterized by a high degree of morphological s ...200718095455
functional analysis of genes encoding putative oxidoreductases in aspergillus oryzae, which are similar to fungal fructosyl-amino acid oxidase.we found 11 genes (fao1-11) encoding putative oxidoreductases in the aspergillus oryzae genome, which are similar to fungal fructosyl-amino acid oxidases. the cdnas corresponding to the genes were cloned and expressed in escherichia coli. rfao2 had fructosyl-amino acid oxidase activity, whereas rfao1 did not show any enzyme activity, even though the deduced amino acid sequence of fao1 is identical to that of one of the fructosyl-amino acid oxidase isozymes from aspergillus oryzae. rfao7 and rfao ...200718086445
characterisation of 3-methylorcinaldehyde synthase (mos) in acremonium strictum: first observation of a reductive release mechanism during polyketide biosynthesis.isolation and sequencing of a pks gene isolated from xenovulene-producing cultures of acremonium strictum indicated the presence of nt-, ks-, at-, pt-, c-met- and r-domains; heterologous expression in aspergillus oryzae resulted in the production of 3-methylorcinaldehyde, demonstrating the role of the terminal reductase domain in product release.200717912413
a proteomics strategy to discover beta-glucosidases from aspergillus fumigatus with two-dimensional page in-gel activity assay and tandem mass spectrometry.economically competitive production of ethanol from lignocellulosic biomass by enzymatic hydrolysis and fermentation is currently limited, in part, by the relatively high cost and low efficiency of the enzymes required to hydrolyze cellulose to fermentable sugars. discovery of novel cellulases with greater activity could be a critical step in overcoming this cost barrier. beta-glucosidase catalyzes the final step in conversion of glucose polymers to glucose. despite the importance, only a few be ...200718020405
[expression of pectin lyase 1 from aspergillus oryzae in escherichia coli].pectin lyases from aspergillus oryzae and aspergillus niger are usually used for the production of traditional fermented foods, but these fungi produce less pectinases under natural conditions. the cdna coding mature pell (without signal peptide) was amplified from aspergillus oryzae by rt-pcr. pell cdna was cloned into pet-28a ( + ) expression vector, then was transformed into e. coli turner (de3) plac i cells to express pell with 6-his tag. for improving the efficiency of pell expression in e. ...200718051868
[lipase-catalyzed production of biodiesel from high acid value waste oil with ultrasonic assistant].biodiesel fuel produced with the enzyme-catalyzed esterification and transesterification of high acid value waste oil through ultrasonic assistant was explored. propyl oleate, biodiesel, converted from high acid value waste oil and 1-proponal catalyzed with immobilized lipases from candida antarctica and aspergillus oryzae in conditions of ultrasonic assistant. commercial immobilized lipase novozym 435 from c. antarctica was used as biocatalyst catalyzing high acid value waste oil and 1-proponal ...200718257248
evaluation of certain food additives and contaminants.this report represents the conclusions of a joint fao/who expert committee convened to evaluate the safety of various food additives, including flavouring agents, with a view to recommending acceptable daily intakes (adis) and to preparing specifications for identity and purity. the committee also evaluated the risk posed by two food contaminants, with the aim of advising on risk management options for the purpose of public health protection. the first part of the report contains a general discu ...200718551832
tannase production by solid state fermentation of cashew apple bagasse.the ability of aspergillus oryzae for the production of tannase by solid state fermentation was investigated using cashew apple bagasse (cab) as substrate. the effect of initial water content was studied and maximum enzyme production was obtained when 60 ml of water was added to 100.0 g of cab. the fungal strain was able to grow on cab without any supplementation but a low enzyme activity was obtained, 0.576 u/g of dry substrate (g(ds)). optimization of process parameters such as supplementation ...200718478425
genomics of industrial aspergilli and comparison with toxigenic relatives.aspergillus oryzae has been used in japanese fermentation industries for more than a thousand years. the species produces large amounts of various hydrolytic enzymes and has been successfully applied to modern biotechnology. the size of the a. oryzae genome (37.5 mb) is very close to that of a. flavus and a. niger, and 20-30% larger than that of either a. nidulans or a. fumigatus. a. oryzae and a. flavus have exactly the same number of aspartic proteinase genes, of which each orthologous pair sh ...200818798040
entrapment of beta-galactosidase in polyvinylalcohol hydrogel.beta-galactosidase isolated from aspergillus oryzae was immobilized in lens-shaped polyvinylalcohol capsules (with activity 25 u g(-1)) giving 32% of its original activity. immobilization did not change the ph optimum (4.5) of lactose hydrolysis. the relative enzyme activity during product inhibition testing was, in average, 10% higher for immobilized enzyme. no decrease of activity was observed after 35 repeated batch runs and during 530 h of continuous hydrolysis of lactose (10%, w/v) at 45 de ...200818043870
genomics of aspergillus oryzae: learning from the history of koji mold and exploration of its future.at a time when the notion of microorganisms did not exist, our ancestors empirically established methods for the production of various fermentation foods: miso (bean curd seasoning) and shoyu (soy sauce), both of which have been widely used and are essential for japanese cooking, and sake, a magical alcoholic drink consumed at a variety of ritual occasions, are typical examples. a filamentous fungus, aspergillus oryzae, is the key organism in the production of all these traditional foods, and it ...200818820080
enhancement of the alcoholytic activity of alpha-amylase amya from thermotoga maritima msb8 (dsm 3109) by site-directed mutagenesis.amya, an alpha-amylase from the hyperthermophilic bacterium thermotoga maritima, is able to hydrolyze internal alpha-1,4-glycosidic bonds in various alpha-glucans at 85 degrees c as the optimal temperature. like other glycoside hydrolases, amya also catalyzes transglycosylation reactions, particularly when oligosaccharides are used as substrates. it was found that when methanol or butanol was used as the nucleophile instead of water, amya was able to catalyze alcoholysis reactions. this capabili ...200818552192
dephosphorylation and quantification of organic phosphorus in poultry litter by purified phytic-acid high affinity aspergillus phosphohydrolases.extracellular phosphohydrolases mediate the dephosphorylation of phosphoesters and influence bioavailability and loss of agricultural p to the environment to pose risks of impairment of sensitive aquatic ecosystems. induction and culture of five strains of aspergillus were conducted to develop a source of high-affinity and robust phosphohydrolases for detecting environmental p and quantifying bioactive p pools in heterogeneous environmental specimens. enzyme stability and activity against organi ...200818555509
characterization of an alpha-l-rhamnosidase from aspergillus kawachii and its gene.an alpha-l-rhamnosidase was purified by fractionating a culture filtrate of aspergillus kawachii grown on l-rhamnose as the sole carbon source. the alpha-l-rhamnosidase had a molecular mass of 90 kda and a high degree of n-glycosylation of approximately 22%. the enzyme exhibited optimal activity at ph 4.0 and temperature of 50 degrees c. further, it was observed to be thermostable, and it retained more than 80% of its original activity following incubation at 60 degrees c for 1 h. its t (50) val ...200818633609
[polarized distribution of intracellular organelles involved in vesicular trafficking in filamentous fungal cells]. 200818453152
a wheat biorefining strategy based on solid-state fermentation for fermentative production of succinic acid.in this study, a novel generic feedstock production strategy based on solid-state fermentation (ssf) has been developed and applied to the fermentative production of succinic acid. wheat was fractionated into bran, gluten and gluten-free flour by milling and gluten extraction processes. the bran, which would normally be a waste product of the wheat milling industry, was used to produce glucoamylase and protease enzymes via ssf using aspergillus awamori and aspergillus oryzae, respectively. the r ...200818434138
characterization of the aspergillus niger prtt, a unique regulator of extracellular protease encoding genes.expression of several aspergillus niger genes encoding major secreted, but not vacuolar, protease genes including the major acid protease gene pepa, was shown to be affected in the previously isolated a. niger protease mutant, ab1.13 [mattern, i.e., van noort, j.m., van den berg, p., archer, d.a., roberts, i.n., hondel, c.a.m.j.j., 1992. isolation and characterization of mutants of aspergillus niger deficient in extracellular proteases. molecular & general genetics 2, 332-336]. complementation c ...200818930158
preparation and comparative characterization of immobilized aspergillus oryzae expressing fusarium heterosporum lipase for enzymatic biodiesel production.in this paper, we provide the first report of utilizing recombinant fungal whole cells in enzymatic biodiesel production. aspergillus oryzae, transformed with a heterologous lipase-encoding gene from fusarium heterosporum, produced fully processed and active forms of recombinant f. heterosporum lipase (fhl). cell immobilization within porous biomass support particles enabled the convenient usage of fhl-producing a. oryzae as a whole-cell biocatalyst for lipase-catalyzed methanolysis. the additio ...200818795281
direct ethanol production from barley beta-glucan by sake yeast displaying aspergillus oryzae beta-glucosidase and endoglucanase.three beta-glucosidase- and two endoglucanase-encoding genes were cloned from aspergillus oryzae, and their gene products were displayed on the cell surface of the sake yeast, saccharomyces cerevisiae gri-117-uk. gri-117-uk/pudb7 displaying beta-glucosidase ao090009000356 showed the highest activity against various substrates and efficiently produced ethanol from cellobiose. on the other hand, gri-117-uk/pudcb displaying endoglucanase ao090010000314 efficiently degraded barley beta-glucan to glu ...200818640601
stable repeated-batch production of recombinant dye-decolorizing peroxidase (rdyp) from aspergillus oryzae.recombinant aspergillus oryzae expressing a dye-decolorizing peroxidase gene (dyp) was cultivated for repeated-batch production of recombinant dye-decolorizing peroxidase (rdyp) using maltose as a carbon source. high-level rdyp activity in limitation of carbon and nitrogen sources was maintained stably for 26 cycles of repeated 1-d batches of a. oryzae pellets without any additional ph control. cultures maintained at 4 degrees c for 20 d resumed rdyp production following a single day of incubati ...200818640612
an open label study to determine the effects of an oral proteolytic enzyme system on whey protein concentrate metabolism in healthy males.current research suggests that protein intake of 1.5 - 2.8 g/kg/day (3.5 times the current recommended daily allowance) is effective and safe for individuals trying to increase or maintain lean muscle mass. to achieve these levels of daily protein consumption, supplementing the diet with processed whey protein concentrate (wpc) in liquid form has become a popular choice for many people. some products have a suggested serving size as high as 50 g of protein. however, due to possible inhibition of ...200818652668
identification and cloning of a gene encoding tannase (tannin acylhydrolase) from lactobacillus plantarum atcc 14917(t).the gene tanlpl, encoding a novel tannase enzyme (tanlpl), has been cloned from lactobacillus plantarum atcc 14917(t). this is the first report of a tannase gene cloned from a bacterial source other than from staphylococcus lugdunensis, which has been reported elsewhere. the open reading frame of tanlpl, spanning 1410 bp, encoded a 469-amino-acid protein that showed 28.8% identity to the tannase of s. lugdunensis with several commonly conserved sequences. these sequences could not be found in pu ...200818653299
improvement of aspergillus oryzae for hyperproduction of endoglucanase: expression cloning of cmc-1 gene of aspergillus aculeatus.fi-carboxymethylcellulase (cmc1; family 12) is one of the endoglucanases of aspergillus aculeatus and consists of single polypeptide chain of 221 amino acids. the cmc1 gene was expressed in aspergillus oryzae niad300 (niad-) under promoter 8142. the plasmid pcmg14 carrying the cmc1 gene at psti site was used as a source of the gene (920 bp) and aspergillus oryzae was successfully transformed by the plasmid pnan-cmc1 (harboring cmc1 gene). the plasmid was integrated in aspergillus oryzae niad300 ...200818661108
effects of a casein hydrolysate prepared from aspergillus oryzae protease on adjuvant arthritis in rats.we evaluated the effects of a casein hydrolysate (ch) prepared from aspergillus oryzae protease on rat adjuvant arthritis, a model of human rheumatoid arthritis. ch was administered orally once a day to the animals for 22 d after the adjuvant injection. ch suppressed swelling in the adjuvant-uninjected hind paws, and a higher dose of ch suppressed the increase in arthritic score and swelling of the adjuvant-injected hind paws. a histopathological examination revealed evidence that the higher dos ...200818685219
a comparison of the unfolded protein response in solid-state with submerged cultures of aspergillus oryzae.the unfolded protein response (upr) is a regulatory system to maintain the homeostasis of er functions. here we report a comparison of express levels of upr relevant genes in aspergillus oryzae between solid-state and submerged cultivation. the results were that up-regulation of the upr mechanism in solid-state culture was higher than in submerged culture (heat-shock or non-stress conditions). this might have been a result of changing culture conditions.200818997417
therapeutic effects of a fermented soy product on peanut hypersensitivity is associated with modulation of t-helper type 1 and t-helper type 2 responses.immubalance is a koji fungus (aspergillus oryzae) and lactic acid fermented soybean product. this unique production process is believed to create a food supplement that helps to induce or maintain normal immune response.200818705693
codon optimization increases steady-state mrna levels in aspergillus oryzae heterologous gene expression.we investigated the effect of codon optimization on the expression levels of heterologous proteins in aspergillus oryzae, using the mite allergen der f 7 as a model protein. a codon-optimized der f 7 gene was synthesized according to the frequency of codon usage in a. oryzae by recursive pcr. both native and optimized der f 7 genes were expressed under the control of a high-level-expression promoter with their own signal peptides or in a fusion construct with a. oryzae glucoamylase (glaa). codon ...200818791013
multiple gene disruptions by marker recycling with highly efficient gene-targeting background (deltaligd) in aspergillus oryzae.previously we reported that double disruption of the proteinase genes (tppa and pepe) improved heterologous protein production by aspergillus oryzae (jin et al. appl microbiol biotechnol 76:1059-1068, 2007). since a. oryzae has 134 protease genes, the number of auxotrophy in a single host is limited for multiple disruptions of many protease genes. in order to rapidly perform multiple gene disruptions in a. oryzae, we generated the marker recycling system in highly efficient gene-targeting backgr ...200818574559
construction of a citrinin gene cluster expression system in heterologous aspergillus oryzae.filamentous fungi are considered an attractive resource for the discovery and production of bioactive compounds. to facilitate molecular breeding, biosynthetic genes must be rapidly identified. but, even after the chemical structure of a compound is identified, finding the corresponding biosynthetic genes in the fungal genome still remains a challenge. in an attempt to overcome this difficulty and to easily characterize each gene in a cluster, we constructed a heterologous expression system usin ...200819111642
isolation of industrial strains of aspergillus oryzae lacking ferrichrysin by disruption of the dffa gene.based on studies using laboratory strains, the efficiency of gene disruption in aspergillus oryzae, commonly known as koji mold, is low; thus, gene disruption has rarely been applied to the breeding of koji mold. to evaluate the efficiency of gene disruption in industrial strains of a. oryzae, we produced ferrichrysin biosynthesis gene (dffa) disruptants using three different industrial strains as hosts. pcr analysis of 438 pyrithiamine-resistant transformants showed dffa gene disruption efficie ...200819111645
construction of an aspergillus oryzae cell-surface display system using a putative gpi-anchored protein.a novel cell-surface display system was constructed in aspergillus oryzae. each of the five genes encoding the putative cell-wall-localized protein from the a. oryzae genome was cloned and these cell-surface anchor functions were examined by fusion to the c-terminal of the green fluorescent protein (gfp). using the mp1 and cwp proteins as anchor proteins, gfp signals were strongly observed on the cell surface of recombinant a. oryzae. when these proteins were used as anchor proteins for cell-sur ...200818813924
isolation and characterization of an extracellular antimicrobial protein from aspergillus oryzae.a 17 kda antimicrobial protein was isolated from growth medium containing the filamentous fungus aspergillus oryzae by extracting the supernatants from the culture media, ion exchange chromatography on cm-sepharose, and c18 reverse-phase high-performance liquid chromatography. this antimicrobial protein, which we considered to be an extracellular antimicrobial protein from a. oryzae (exap-ao17), possessed antimicrobial activity but lacked hemolytic activity. the exap-ao17 protein strongly inhibi ...200818803391
beneficial effects of oral tilactase on patients with hypolactasia.a lactose-free diet is commonly prescribed to subjects with hypolactasia. we tested the effectiveness of a single ingestion of tilactase (a beta-d-galactosidase from aspergillus oryzae) in adults with hypolactasia, previously assessed by lactose h(2)-breath test.200819021701
generation of large chromosomal deletions in koji molds aspergillus oryzae and aspergillus sojae via a loop-out recombination.we established a technique for efficiently generating large chromosomal deletions in the koji molds aspergillus oryzae and a. sojae by using a ku70-deficient strain and a bidirectional marker. the approach allowed deletion of 200-kb and 100-kb sections of a. oryzae and a. sojae, respectively. the deleted regions contained putative aflatoxin biosynthetic gene clusters. the large genomic deletions generated by a loop-out deletion method (resolution-type recombination) enabled us to construct multi ...200818952883
efficient production and partial characterization of aspartyl aminopeptidase from aspergillus oryzae.aspartyl aminopeptidase (dap) has a high degree of substrate specificity, degrading only amino-terminal acidic amino acids from peptides. therefore, attention is focused here on the efficient production of this enzyme by a recombinant aspergillus oryzae and characterization of its biochemical properties.200818828788
enzymatically hydrolyzed lactotripeptides do not lower blood pressure in mildly hypertensive subjects.several placebo-controlled clinical studies suggest that products containing isoleucyl-prolyl-proline and valyl-prolyl-proline are able to lower blood pressure without adverse effects. the most efficient way of producing high concentrations of these lactotripeptides (ltps) is enzymatic hydrolysis of dairy protein (casein) with the use of a mixture of several enzymes derived from the nongenetically modified organism aspergillus oryzae, including proteases and peptidases. to date, no large studies ...200819064533
development and evaluation of a real-time quantitative pcr assay for aspergillus flavus.aspergillus flavus is a ubiquitous mold and the most common mold contaminating foodstuffs. many strains of a. flavus produce aflatoxins. in addition it is an allergen and an opportunistic pathogen of animals and plants. a. flavus often is underestimated in traditional culture analyses due to the expertise required and the cost associated with speciating members of the genus aspergillus. the goal of this study was to develop and validate a primer and probe set for the rapid detection and quantita ...200818959152
some studies of alpha-amylase production using aspergillus oryzae.the extracellular alpha-amylase production by aspergillus oryzae was studied in submerged fermentation using an adlof-kuhner orbital shaker. the effect of initial ph values in the range of 4 to 7.5 on enzyme production was investigated and initial ph medium of 6.2 +/- 0.1 resulted in enhanced alpha-amylase production. the effect of carbon and nitrogen source and composition was examined and it has been observed that corn starch concentration of 15 g l(-1) has sound effect on enzyme production. t ...200819260332
identification of a novel polyketide synthase-nonribosomal peptide synthetase (pks-nrps) gene required for the biosynthesis of cyclopiazonic acid in aspergillus oryzae.cyclopiazonic acid (cpa) is a mycotoxin produced by several strains of penicillium and aspergillus species. aspergillus oryzae strains used in fermented foods do not produce cpa; however, several wild-type a. oryzae strains produce cpa. here, we identified a novel polyketide synthase-nonribosomal peptide synthetase (pks-nrps) gene involved in cpa production by comparing the telomere-adjacent region of a cpa-producing strain (a. oryzae nbrc 4177) with that of a nonproducing strain (a. oryzae rib4 ...200818854220
late stage oxidations during the biosynthesis of the 2-pyridone tenellin in the entomopathogenic fungus beauveria bassiana.late stage oxidations during the biosynthesis of the 2-pyridone tenellin in the insect pathogenic fungus beauveria bassiana were investigated by a combination of gene knockout, antisense rna, and gene coexpression studies. open reading frames (orf) 3 and 4 of the tenellin biosynthetic gene cluster were previously shown to encode a trans-acting enoyl reductase and a hybrid polyketide synthase nonribosomal peptide synthetase (pks-nrps), respectively, which together synthesize the acyltetramic acid ...200819067514
codon optimization prevents premature polyadenylation of heterologously-expressed cellulases from termite-gut symbionts in aspergillus oryzae.to achieve high expression of glycoside hydrolase family 45 endoglucanase (rssym45eg1) from a symbiotic protist of the termite reticulitermes speratus, synthetic sequence rssym45eg1-co, in which the codon usage was adjusted to that of the highly-expressed tef1 gene encoding translation elongation factor 1alpha, was prepared and introduced into a. oryzae. the transcript level of rssym45eg1-co was 1.8-fold higher than that of rssym45eg1. in cells harboring rssym45eg1, but not rssym45eg1-co, trunca ...200819164877
aspergillus flavus genomics as a tool for studying the mechanism of aflatoxin formation.aspergillus flavus is a weak pathogen that infects plants, animals and humans. when it infects agricultural crops, however, it produces one of the most potent carcinogens known (aflatoxins). to devise strategies to control aflatoxin contamination of pre-harvest agricultural crops and post-harvest grains during storage, we launched the a. flavus genomics program. the major objective of this program is the identification of genes involved in aflatoxin biosynthesis and regulation, as well as in pat ...200819238624
authentic heterologous expression of the tenellin iterative polyketide synthase nonribosomal peptide synthetase requires coexpression with an enoyl reductase.the tens gene encoding tenellin synthetase (tens), a 4239-residue polyketide synthase nonribosomal-peptide synthetase (pks-nrps) from beauveria bassiana, was expressed in aspergillus oryzae m-2-3. this led to the production of three new compounds, identified as acyl tetramic acids, and numerous minor metabolites. consideration of the structures of these compounds indicates that the putative c-terminal thiolester reductase (r) domain does not act as a reductase, but appears to act as a dieckmann ...200818266306
improved annotation through genome-scale metabolic modeling of aspergillus oryzae.since ancient times the filamentous fungus aspergillus oryzae has been used in the fermentation industry for the production of fermented sauces and the production of industrial enzymes. recently, the genome sequence of a. oryzae with 12,074 annotated genes was released but the number of hypothetical proteins accounted for more than 50% of the annotated genes. considering the industrial importance of this fungus, it is therefore valuable to improve the annotation and further integrate genomic inf ...200818500999
identification of ace-inhibitory peptides in salt-free soy sauce that are transportable across caco-2 cell monolayers.in present study, we aimed to identify angiotensin i-converting enzyme (ace)-inhibitory peptides from a salt-free soy sauce (sfs), a newly developed antihypertensive seasoning obtained by aspergillus oryzae fermentation of soybean in the absence of salt, which can be transported through caco-2 cell monolayers. through an ussing transport investigation of sfs across caco-2 cell monolayers, three di-peptides, ala-phe, phe-ile and ile-phe, were successfully identified from the sfs as transportable ...200818160180
role of n-terminal 28-amino-acid region of rhizopus oryzae lipase in directing proteins to secretory pathway of aspergillus oryzae.to develop a new approach for improving heterologous protein production in aspergillus oryzae, we focused on the functional role of the n-terminal region of rhizopus oryzae lipase (rol). several n-terminal deletion variants of rol were expressed in a. oryzae. interestingly, a segment of 28 amino acids from the c-terminal region of the propeptide (n28) was found to be critical for secretion of rol into the culture medium. to further investigate the role of n28, the rol secretory process was visua ...200818496686
a microtitre plate assay for measuring glycosidase activity.glycosidases perform a wide range of functions in physiology and pathology, and are potential targets for the treatment of diseases such as influenza, cancer, aids and diabetes. this paper reports a convenient discontinuous colourimetric assay for the measurement of glycosidase activity. the assay utilises 4-nitrophenyl- substrates and quantities of product are determined by measuring absorbance at 405 nm. this assay is performed in a 96 well microtitre plate and has been used to characterise th ...200818341264
a highly efficient gene-targeting system for aspergillus parasiticus.to establish a system that greatly increases the gene-targeting frequency in aspergillus parasiticus.200818346134
alcoholic chestnut fermentation in mixed culture. compatibility criteria between aspergillus oryzae and saccharomyces cerevisiae strains.the main objective of the present work consisted in the transfer to the case of the chestnut of a rice fermentative process that carried out to the japanese traditional way to lead to an alcoholic bagasse, the moromi, capable of obtaining distilled. this way, selection assays of amylolitic aspergillus oryzae strains and studies of compatibility between microfungi and yeast were carried out. these mixed cultivations were performed operating in batch submerged culture. later on, using solid state ...200818289846
cloning and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene and the use of its promoter for expression in myrothecium gramineum, a novel expression host.at our laboratory, research has focused on the development of myrothecium gramineum as a novel expression host. the glyceraldehyde-3-phosphate dehydrogenase (gpd)-promoter of m. gramineum was isolated and characterized (genbank accession number ef486690). in order to prove its functionality and to explore the potential of m. gramineum as a novel fungal expression host, use of this gpd-promoter for the expression of a fungal alpha-amylase was investigated. myrothecium gramineum was transformed wi ...200818294194
acid activation of protyrosinase from aspergillus oryzae: homo-tetrameric protyrosinase is converted to active dimers with an essential intersubunit disulfide bond at acidic ph.aspergillus oryzae protyrosinase (pro-ty) has a unique feature that the proenzyme is activated under conditions of acidic ph. the pro-ty was inactive at ph 7.0. the latent enzyme was activated at ph 3.0, and was slightly activated by sodium dodecyl sulfate (sds). the molecular masses of the pro-ty and acid-activated tyrosinase (acid-ty) were 266 and 165 kda, respectively, as estimated by gel-filtration chromatography. the cd spectra showed that the tertiary and/or quaternary structure was change ...200818353147
gene structure dynamics and divergence of the polygalacturonase gene family of plants and fungus.whole copies of the polygalacturonase (pg) genes from rice (oryza sativa subsp. japonica) and a filamentous fungus (aspergillus oryzae) were isolated. the orthologs of the rice pgs were also retrieved from other plant species. the 106 plant pgs analyzed were divided into 5 clades, a, b, c, d, and e. the fungus pgs were classified into 3 clades, of which one formed a loose cluster with clade e of the plant pgs. four domain motifs (i, ii, iii, iv) were identified in all pgs. motifs ii and iii were ...200818356937
purification of alpha-galactosidase and invertase by three-phase partitioning from crude extract of aspergillus oryzae.alpha-galactosidase and invertase were accumulated in a coherent middle phase in a three-phase partitioning system under different conditions (ammonium sulphate, ratio of tert-butanol to crude extract, temperature and ph). alpha-galactosidase and invertase were purified 15- and 12-fold with 50 and 54% activity recovery, respectively. the fractions of interfacial precipitate arising from the three-phase partitioning were analyzed by sds-page. both purified preparations showed electrophoretic homo ...200818414794
fusarielin a as an anti-angiogenic and anti-proliferative agent: basic biological characterization.fusarielin a shows anti-angiogenic activity in the human umbilical vein endothelial cell (huvec) tube formation assay. structural development studies indicated the importance of the hydroxyl groups in this molecule. a 3h-labeled derivative and a fluorescent affinity-labeling agent were prepared and used to examine the cellular distribution and biological behavior of fusarielin a.200818310939
methylation in methanol-water mixtures: the effect of solvent composition and high pressure.the effect of pressure (0.1 to 400 mpa) and solvent composition (methanol concentration of 5 to 30%) on the synthesis of beta-methylgalactoside was studied. galactose was used as a reactant and the reaction was catalyzed by beta-galactosidase from aspergillus oryzae. under the applied conditions the enzyme was sufficiently stable and the reaction equilibrium was reached. higher methanol concentrations obviously influenced the product yield positively due to an increase in reactant concentration ...200818329783
immunoreactivity and amino acid content of fermented soybean products.food allergy has become a public health problem that continues to challenge both the public and the food industry. the objective of this research was the detection and quantification of the major human allergenic soy proteins and to study the reduction in immunoreactivity and improvement of amino acid content after fermentation of soybean flour. fermentation was carried out in the solid state of cracked seeds inoculated with aspergillus oryzae, rhizopus oryzae, and bacillus subtilis and in the l ...200818072744
high-level expression, purification and characterization of recombinant aspergillus oryzae alkaline protease in pichia pastoris.the alkaline protease gene from aspergillus oryzae was cloned, and then it was successfully expressed in the heterologous pichia pastoris gs115 with native signal peptide or alpha-factor secretion signal peptide. the yield of the recombinant alkaline protease with native signal peptide was about 1.5-fold higher than that with alpha-factor secretion signal peptide, and the maximum yield of the recombinant alkaline protease was 513 mg/l, which was higher than other researches. the recombinant alka ...200818226921
effect of cultivation ph and agitation rate on growth and xylanase production by aspergillus oryzae in spent sulphite liquor.the effects of cultivation ph and agitation rate on growth and extracellular xylanase production by aspergillus oryzae nrrl 3485 were investigated in bioreactor cultures using spent sulphite liquor (ssl) and oats spelts xylan as respective carbon substrates. xylanase production by this fungus was greatly affected by the culture ph, with ph 7.5 resulting in a high extracellular xylanase activity in the ssl-based medium as well as in a complex medium with xylan as carbon substrate. this effect, th ...200818239946
monitoring global gene expression of proteases and improvement of human lysozyme production in the nptb gene disruptant of aspergillus oryzae.aspergillus oryzae has numerous protease genes that might cause proteolytic degradation of heterologously-produced proteins. the productivity of the heterologous protein can be improved by protease gene disruption, but it is difficult to select disruption targets efficiently. in this study, we monitored the expression of 132 protease genes by dna microarray. a group of protease genes up-regulated during cultivation was identified by clustering analysis. in this protease group, the nptb gene enco ...200818256487
two types of beta-fructofuranosidases from aspergillus oryzae kb.aspergillus oryzae kb produces two types of beta-fructofuranosidases, f1 and f2. f1 produces 1-kestose, nystose, and fructosyl nystose from sucrose through its transfructosylation action. f2 hydrolyzes sucrose to glucose and fructose. n-terminal amino acid sequences of the purified enzymes were dynaappnlst for f1 and ysgdlrpq for f2. each enzyme encoding gene was identified in the genome of aspergillus oryzae. although the kb strain showed a higher production of f2 than f1 in a low sucrose liqui ...200818088091
solid-state fermentation with fungi to enhance the antioxidative activity, total phenolic and anthocyanin contents of black bean.in the present study, solid-state fermentation of black bean with various gras filamentous fungi including aspergillus awamori, aspergillus oryzae, aspergillus sojae, rhizopus azygosporus and rhizopus sp. no. 2 was preformed to prepared koji. mycelial propagation of starter organisms and antioxidative activity, including alpha-alpha-diphenyl-2-picyl-hydoxyl (dpph) radicals, fe2+-chelating ability, and reducing activity, were examined. depending upon the starter organism, various amounts of mycel ...200818031859
application of a statistical design to the optimization of parameters and culture medium for alpha-amylase production by aspergillus oryzae cbs 819.72 grown on gruel (wheat grinding by-product).the production optimization of alpha-amylase (e.c.3.2.1.1) from aspergillus oryzae cbs 819.72 fungus, using a by-product of wheat grinding (gruel) as sole carbon source, was performed with statistical methodology based on three experimental designs. the optimisation of temperature, agitation and inoculum size was attempted using a box-behnken design under the response surface methodology. the screening of nineteen nutrients for their influence on alpha-amylase production was achieved using a pla ...200818180155
fungal mycelia show lag time before re-growth on endogenous carbon.nutrient starvation is a common occurrence for filamentous fungi. to better understand the effects of starvation, we used a parallel plate flow chamber to study individual fungal mycelia when subjected to a step change in glucose concentration. we report the presence of a finite "lag time" in starved mycelia during which they ceased to grow/extend while switching from growth on exogenous carbon to re-growth on endogenous carbon. this lag time precedes other morphological or physiological changes ...200818183629
new, efficient synthesis of oseltamivir phosphate (tamiflu) via enzymatic desymmetrization of a meso-1,3-cyclohexanedicarboxylic acid diester.a new, enantioselective synthesis of the influenza neuraminidase inhibitor prodrug oseltamivir phosphate 1 (tamiflu) and its enantiomer ent-1 starting from cheap, commercially available 2,6-dimethoxyphenol 10 is described. the main features of this approach comprise the cis-hydrogenation of 5-(1-ethyl-propoxy)-4,6-dimethoxy-isophthalic acid diethyl ester (6a) and the desymmetrization of the resultant all-cis meso-diesters 7a and 7b, respectively. enzymatic hydrolysis of the meso-diester 7b with ...200818517254
high prevalence of fastidious bacteria in 1520 cases of uveitis of unknown etiology.the etiologic evaluation of uveitis is frequently unsuccessful when noninvasive methods are used. we conducted a prospective study to evaluate systematic screening for pathogens of uveitis. all patients with uveitis referred to the participating tertiary ophthalmology departments from january 2001 to september 2007 underwent intraocular and serum specimen collection. the standardized protocol for laboratory investigations included universal polymerase chain reaction (pcr)-based detection of any ...200818520326
deletion analysis of the catalase-encoding gene (catb) promoter from aspergillus oryzae.the catalase-encoding gene (catb) is expressed strongly in aspergillus oryzae. to identify the transcription regulatory elements involved in strong expression, we did promoter deletion analysis using beta-glucuronidase (gus) as a reporter and an electrophoretic gel mobility shift assay (emsa) systematically. the deletion 200-bp sequence from -1,000 to -800 in the 1,400-bp catb promoter caused a drastic decrease in gus activity. in addition, emsa implicated a 45-bp element from -1,000 to -956 con ...200818175923
elusive origins of the extra genes in aspergillus oryzae.the genome sequence of aspergillus oryzae revealed unexpectedly that this species has approximately 20% more genes than its congeneric species a. nidulans and a. fumigatus. where did these extra genes come from? here, we evaluate several possible causes of the elevated gene number. many gene families are expanded in a. oryzae relative to a. nidulans and a. fumigatus, but we find no evidence of ancient whole-genome duplication or other segmental duplications, either in a. oryzae or in the common ...200818725939
corncob-induced endo-1,4-beta-d-xylanase of aspergillus oryzae mtcc 5154: production and characterization of xylobiose from glucuronoxylan.eight different fungi were cultivated in a peptone-yeast extract medium containing 1% oat spelt xylan (osx) to evaluate endo-1,4-beta-xylanase secretion for xylooligosaccharide (xos) production. aspergillus oryzae mtcc 5154, aspergillus flavus , aspergillus niger , and aspergillus ochraceus showed significant titers of endoxylanases, which were further used for the production of xos from birch wood xylan (bwx). a. oryzae produced 89.5 +/- 1.13% xos in the hydrolysate at 24 h of reaction. the eff ...200818489109
cloning and expression of a gene encoding gallerimycin, a cysteine-rich antifungal peptide, from eri-silkworm, samia cynthia ricini.a cdna clone encoding gallerimycin was isolated from larval fat body of immunized samia cynthia ricini and named as scr-gallerimycin. in naive larvae, no gene expression was detected, but strongly induced in fat body and hemocytes following immune challenge with bacteria or entomopathogenic fungus beauveria bassiana. strong expression of the gene was also induced by injection of peptidoglycan and zymosan, but very weakly by non-pathogenic fungus aspergillus oryzae. analysis of the sequence upstr ...200818436462
concanavalin a layered calcium alginate-starch beads immobilized beta galactosidase as a therapeutic agent for lactose intolerant patients.a novel therapeutic agent in the form of beta galactosidase immobilized on the surface of concanavalin a layered calcium alginate-starch beads has been developed. immobilized beta galactosidase exhibited significantly very high stability against conditions of digestive system such as ph, salivary amylase, pepsin and trypsin. soluble and immobilized beta galactosidase exhibited same ph-optima. however, the immobilized enzyme retained greater fraction of catalytic activity at higher and lower ph t ...200818439774
lactic fermentation of cellobiose by a yeast strain displaying beta-glucosidase on the cell surface.the aspergillus aculeatus beta-glucosidase 1 (bgl1) gene was expressed in a lactic-acid-producing saccharomyces cerevisiae strain to enable lactic fermentation with cellobiose. the recombinant beta-glucosidase enzyme was expressed on the yeast cell surface by fusing the mature protein to the c-terminal half region of the alpha-agglutinin. the beta-glucosidase expression plasmids were integrated into the genome. three strong promoters of s. cerevisiae, the tdh3, pgk1, and pdc1 promoters, were use ...200818443785
aspergillus oryzae atfb encodes a transcription factor required for stress tolerance in conidia.using an aspergillus oryzae est database, we identified a gene encoding a transcription factor (atfb), which is a member of the atf/creb family. expression of atfb was barely detectable during vegetative growth, but was readily detected during conidiation in solid-state culture. microarray analyses showed that expression of many other genes, including catalase (cata), were downregulated in an atfb-disruptant. the expression of most of these genes was upregulated in the wild-type strain during th ...200818448366
riboswitches for enhancing target gene expression in eukaryotes. 200818338354
isoflavone aglycones production from isoflavone glycosides by display of beta-glucosidase from aspergillus oryzae on yeast cell surface.for efficient production of isoflavone aglycones from soybean isoflavones, we isolated three novel types of beta-glucosidase (bgl1, bgl3, and bgl5) from the filamentous fungi aspergillus oryzae. three enzymes were independently displayed on the cell surface of a yeast saccharomyces cerevisiae as a fusion protein with alpha-agglutinin. three beta-glucosidase-displaying yeast strains hydrolyzed isoflavone glycosides efficiently but exhibited different substrate specificities. among these beta-gluc ...200818340445
enzymatic production of epigallocatechin by using an epigallocatechin gallate hydrolase induced from aspergillus oryzae.catechins are a group of polyphenolic compounds that are antioxidants having beneficial biological activities. there are four main catechins in green tea, and each has its own biological features. in order to fully exploit prominent biological activities of specific catechins and to develop new medicine from catechins, it is necessary to obtain pure catechin preparations by isolation from natural sources, by chemical synthesis, or by biotransformation reactions with high yield and specificity. i ...200818452340
efficient and direct fermentation of starch to ethanol by sake yeast strains displaying fungal glucoamylases.aspergillus oryzae glucoamylases encoded by glaa and glab, and rhizopus oryzae glucoamylase, were displayed on the cell surface of sake yeast saccharomyces cerevisiae gri-117-uk and laboratory yeast s. cerevisiae mt8-1. among constructed transformants, gri-117-uk/pudgaa, displaying glaa glucoamylase, produced the most ethanol from liquefied starch, although mt8-1/pudgar, displaying r. oryzae glucoamylase, had the highest glucoamylase activity on its cell surface.200818460787
a trispecies aspergillus microarray: comparative transcriptomics of three aspergillus species.the full-genome sequencing of the filamentous fungi aspergillus nidulans, aspergillus niger, and aspergillus oryzae has opened possibilities for studying the cellular physiology of these fungi on a systemic level. as a tool to explore this, we are making available an affymetrix genechip developed for transcriptome analysis of any of the three above-mentioned aspergilli. transcriptome analysis of triplicate batch cultivations of all three aspergilli on glucose and xylose media was used to validat ...200818332432
physicochemical characterization of fructooligosaccharides and evaluation of their suitability as a potential sweetener for diabetics.fructooligosaccharides (foss) were prepared from sucrose using fungal fructosyl transferase (ftase) obtained from aspergillus oryzae mtcc 5154. the resulting mixture consisted of glucose (28-30%), sucrose (18-20%) and fructooligosaccharides (50-54%) as indicated by hplc analysis. identification of oligomers present in the mixture of fructooligosaccharides was carried out using nmr spectroscopy and lc-ms. no compounds other than mono-, di-, tri-, tetra- and pentasaccharides were identified in the ...200818005951
multivariate models for prediction of rheological characteristics of filamentous fermentation broth from the size distribution.the main purpose of this article is to demonstrate that principal component analysis (pca) and partial least squares regression (plsr) can be used to extract information from particle size distribution data and predict rheological properties. samples from commercially relevant aspergillus oryzae fermentations conducted in 550 l pilot scale tanks were characterized with respect to particle size distribution, biomass concentration, and rheological properties. the rheological properties were descri ...200818023062
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