Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| viability of microorganisms in fluorouracil and cisplatin small-volume injections. | the ability of various microorganisms to survive in cisplatin injection or fluorouracil injection in small-volume containers was assessed. inoculum suspensions of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, pseudomonas cepacia, candida albicans, or aspergillus niger were injected into polyvinyl chloride minibags containing 20 ml of either fluorouracil injection 50 mg/ml or cisplatin injection 1 mg/ml in 0.9% sodium chloride injection. all minibags were stored at 25 degrees c ... | 1988 | 3400651 |
| protein-mediated fusion of liposomes with microsomal membranes of aspergillus niger: evidence for a complex mechanism dealing with membranous and cytosolic fusogenic proteins. | membrane fusion is a fundamental and wide-spread phenomenon in the functioning of cells. many studies were carried out concerning fusion of plasma membranes as for example cell-cell fusions or uptake by cells of lipid-enveloped viruses. the present study deals with the interaction of intracellular membranes of aspergillus niger with artificial membranes (liposomes). association is monitored by the uptake of radioactive liposomes by fungal microsomal membranes. the discrimination between aggregat ... | 1988 | 3401470 |
| cosolvent effects on gel-entrapped oxidoreductase: the glucose oxidase model. | an intrinsic problem often involved in biotransformations carried out by immobilized cells is the poor solubility of substrate and product in water. increase in hydrophobic substrate availability to such gel-entrapped cells may be attained by the replacement of a fraction of the aqueous medium by water-miscible solvents (cosolvents). the introduction of cosolvents results in increased solubility, but may simultaneously affect enzymic activity and stability. recently, criteria and guidelines for ... | 1988 | 3408562 |
| aflatoxins and fungal flora in lentil (lens esculenta l.). | fungal flora of 20 samples of lentil seeds collected from assiut governorate, egypt, were studied. seventeen genera and 13 species were isolated on glucose- (15 genera and 27 species), cellulose- (15 genera and 25 species)- czapek's agar media at 28 degrees c. the most common species were as follows: on glucose-czapek's agar, aspergillus fumigatus, a. niger, a. flavus, a. terreus, penicillium notatum and rhizopus oryzae and on cellulose agar, a. fumigatus, a. niger, a. flavus and p. notatum. thi ... | 1988 | 3419482 |
| nadph mediates the inactivation of bovine liver catalase by monochloroamine. | bovine liver catalase suffered a biphasic inactivation when exposed to nh2cl. a rapid and irreversible phase of activity loss was followed by a much slower and reversible inactivation. removal of tightly bound nadph from the enzyme decreased the extent of the rapid phase; whereas addition of nadph augmented it. the catalase from aspergillus niger, which does not contain bound nadph, was not nearly as sensitive toward nh2cl as was the liver enzyme and was not sensitized by added nadph. nadph is o ... | 1988 | 3421706 |
| production and characterization of a murine monoclonal antibody to aspergillus fumigatus antigen having igg- and ige-binding activity. | by employing hybridoma technology, a monoclonal antibody against aspergillus fumigatus was produced. this antibody, isotyped as igm k, reacted with 12 of 16 antigens extracted from 9 different a. fumigatus strains. this antibody also reacted with all 3 aspergillus flavus antigens studied, but not with aspergillus niger, aspergillus terreus, penicillium notatum or candida albicans antigens. western blot analysis indicated that this antibody reacted with two concanavalin a (con-a) binding bands of ... | 1988 | 3045013 |
| [mycologic analyses of house dust in patients with mycotic infections of the paranasal sinuses and in healthy probands]. | dust samples from 30 patients suffering from an aspergillus sinusitis and from 20 healthy persons were analysed for moulds as a possible cause of mycotic sinusitis. questionnaires were also distributed to assess the ecological environment of the patients. mycological analyses of the dust samples showed no statistical difference in the presence of moulds, especially those of aspergillus fumigatus and aspergillus niger. both moulds were found to roughly the same extent in 75%-95% of subjects. the ... | 1988 | 3045056 |
| [production by fungi of the genera aspergillus, acremonium, verticillium of extracellular proteases which coagulate blood plasma and lyse blood clots]. | among 24 fungal strains belonging to the genera aspergillus, acremonium and verticillium, eight strains synthesized proteases with the coagulating activity. the most active strains producing such proteases were found among aspergillus species. the fibrinolytic activity was detected in 12 strains of the fungi. proteases with the fibrinolytic activity differed in their sensitivity to plasma inhibitors and the least sensitive proteases were found in a. niger and a. flavus. some fungal strains synth ... | 1988 | 3062332 |
| binding of fibrinogen to the pathogenic aspergillus species. | the binding of human fibrinogen to the pathogenic aspergilli was investigated in vitro by different procedures using either fibrinogen in solution or fixed, insolubilized fibrinogen. binding of fibrinogen was detected at the surface of hyphae and conidia by an immunofluorescence assay. ultrastructural localization of the binding sites was visualized with fibrinogen-sensitized gold particles. the labelling was restricted to the outer cell wall layer of the 'smooth' walled conidia. quantitative an ... | 1988 | 3073204 |
| nucleotide sequence of the aspergillus niger trpc gene: structural relationship with analogous genes of other organisms. | the nucleotide sequence of the aspergillus niger tryptophan c (trpc) gene was determined. northern hybridization and s1-mapping experiments showed the presence of a 2.6 kb trpc poly(a)+ rna with two very short (5 and 6 nucleotides) noncoding 5'-regions. comparison of the predicted amino acid sequence with that of trp gene proteins of pro- and eukaryotic organisms revealed three functional domains (g, c, f) in the a. niger trpc protein which catalyse the glutamine amidotransferase reaction (gat), ... | 1988 | 2836085 |
| an amds-lacz fusion for studying gene regulation in aspergillus. | a translational fusion has been constructed between the amds gene of aspergillus nidulans and the lacz gene of escherichia coli. sequencing across the fusion junction confirmed the generation of an in-frame fusion at amino acid 34 of amds and a novel protein has been detected in transformants carrying the fusion plasmid. transformants of a. nidulans and aspergillus niger carrying the fusion plasmid were obtained by co-transformation with a second selectable plasmid. these transformants were read ... | 1988 | 2838387 |
| preliminary investigation of in-vitro antimicrobial activity of two nigerian diospyros species (ebenaceae). | the aqueous and alcoholic extracts from the leaves of diospyros bateri and d. monbuttensis were studied for their antimicrobial activities. the alcoholic extracts of the two diospyros spp. showed strong antibacterial activity against a wide range of gram-positive and gram-negative bacteria, while only the aqueous extract of d. bateri showed antibacterial activity against all the bacteria tested. the two fungi included in the study aspergillus niger and candida albicans were found to be resistant ... | 1988 | 2845757 |
| differences in the regulation of aldehyde dehydrogenase genes in aspergillus niger and aspergillus nidulans. | in order to study mechanisms of gene regulation in a. niger, and to compare these to similar systems in a. nidulans, a gene encoding an aldehyde dehydrogenase enzyme has been cloned. in wild-type strains of a. niger the gene shows expression which is regulated by induction and repression. levels of induction by various compounds and the extent of repression under various growth conditions differs from that seen for the a. nidulans alda gene. unlike the a. nidulans alda gene, the a. niger gene ha ... | 1988 | 2846191 |
| calmodulin-dependent multifunctional protein kinase in aspergillus nidulans. | a ca2+/calmodulin (cam)-dependent multifunctional protein kinase has been isolated from aspergillus nidulans and purified to homogeneity. unlike any cam-dependent multifunctional protein kinase described previously, the native enzyme from aspergillus behaves as a monomer. the calculated molecular weight is 41,200. nadodso4/page reveals a single protein band with an apparent mr of 51,000. two-dimensional isoelectric focusing/nadodso4/page of the purified enzyme showed one major and one minor more ... | 1988 | 2835766 |
| the olic3 gene of aspergillus niger: isolation, sequence and use as a selectable marker for transformation. | the olic3 gene of aspergillus niger has been isolated and sequenced. this gene encodes an oligomycin-resistant variant of the mitochondrial atp synthase subunit 9. in transformation experiments the gene can serve as a semi-dominant selectable marker for a. niger. it was possible to recognize transformants in which olic3 had integrated at the homologous olic locus, as opposed to elsewhere in the genome, by observation of phenotypes on medium containing oligomycin. dna sequencing has allowed compa ... | 1988 | 2901295 |
| mic and fungicidal activity of terbinafine against clinical isolates of aspergillus spp. | terbinafine and amphotericin b mics for 90% of strains tested were 1.6 and 0.4 micrograms/ml against aspergillus fumigatus (16 strains), 0.8 and 3.2 micrograms/ml against aspergillus flavus (10 strains), and 0.4 and 1.6 micrograms/ml against aspergillus niger (10 strains), respectively. for all species tested, the minimal inhibitory and fungicidal concentrations for 90% of strains of both drugs were identical and the inoculum size did not have a major effect on the results. | 1988 | 3134851 |
| aspergillus niger peritonitis. | 1988 | 3140093 | |
| protection of cadmium toxicity to b. cereus, e. coli and a. niger by na+, mg++ and ca++ ions. | 10ppm of cadmium apparently seem to extend the lag phase of bacillus cereus and escherichia coli but in reality both 10 and 100ppm decreased the growth with differing intensity. 1.0ppm of cadmium decreased the growth of aspergillus niger, and it failed to grow at 500ppm. however, addition of calcium, magnesium and sodium gave increasing protection against cadmium toxicity for the growth of b. cereus, e. coli an a. niger. it is inferred that environment pollution by cadmium is related to the cati ... | 1988 | 3142161 |
| catabolism of single ring aromatic acids by four aspergillus species. | four species of the genus aspergillus, viz a. fumigatus, a. japonicus, a. niger and a. terreus, decarboxylated, demethoxylated and ring-cleaved aromatic compounds but to different extents. decarboxylation of vanillate occurred before ring-cleavage, which preceded the release of 14co2 from the methoxyl group. a large proportion of labelled carbon from the ring of ferulate and vanillate was found in particulate or trichloracetic acid precipitable material of homogenized fungal mycelium. the four a ... | 1988 | 3144642 |
| preliminary studies on the mechanism of infection and characterization of malassezia pachydermatis in association with canine otitis externa. | a study on the mechanism of infection and the characterization of malassezia pachydermatis in connection with canine otitis externa was conducted. the ability of this yeast to grow uninhibited in intimate contact with the diversity of other microbial isolants of the canine aural canal was demonstrated. canine cerumen seemed to promote the growth of this yeast. although the source of infection is still obscure, m. pachydermatis managed to survive in soil and dust at different temperatures for fou ... | 1988 | 3146702 |
| lactose hydrolysis and oligosaccharide formation catalyzed by beta-galactosidase. kinetics and mathematical modeling. | 1988 | 3147610 | |
| evidence for a cytoplasmic pathway of oxalate biosynthesis in aspergillus niger. | oxalate accumulation of up to 8 g/liter was induced in aspergillus niger by shifting the ph from 6 to 8. this required the presence of pi and a nitrogen source and was inhibited by the protein synthesis inhibitor cycloheximide. exogenously added 14co2 was not incorporated into oxalate, but was incorporated into acetate and malate, thus indicating the biosynthesis of oxalate by hydrolytic cleavage of oxaloacetate. inhibition of mitochondrial citrate metabolism by fluorocitrate did not significant ... | 1988 | 3132096 |
| aspergillus in pulmonary infections. | 1988 | 3133316 | |
| woodman's disease: hypersensitivity pneumonitis from cutting live trees. | a 28-year-old man developed multiple episodes of fever, cough, shortness of breath, and leukocytosis several hours after cutting live oak and maple trees. fungal cultures of wood chips from oak and maple trees were positive for penicillium (three species), paecilomyces sp., aspergillus niger, aspergillus sp., and rhizopus sp. gel-immunodiffusion studies demonstrated serum precipitins to extracts of oak chips, penicillium sp., and paecilomyces sp., and suggested that penicillium sp. and paecilomy ... | 1988 | 3123541 |
| post-processing microflora and the shelf stability of gari marketed in port harcourt. | gari was examined for its post-processing microbial content. aerobic mesophilic bacteria and fungi were isolated from all samples. the total viable bacterial counts ranged from 2.0 x 10(2) to 8.0 x 10(4) cfu/g. fungal counts ranged from 1.0 x 10(2) to 1.5 x 10(4) cfu/g. the total viable counts of fresh samples were much lower than those of market and packaged samples. bacillus, micrococcus and proteus spp. were the bacteria isolated, aspergillus niger, aspergillus flavus and penicillium spp. the ... | 1988 | 3170384 |
| expression analysis vectors for aspergillus niger. | 1988 | 3174446 | |
| [colorimetric determination of glucose oxidase in enzymatic preparations and cellular extracts]. | 1988 | 3175381 | |
| [production of glucose oxidase using aspergillus niger nrrl3]. | 1988 | 3175382 | |
| [carbohydrate metabolism in the fungus aspergillus niger exposed to light]. | 1988 | 3185312 | |
| effect of excimer laser on microbiological organisms. | the effect of radiation emitted from an excimer laser filled with argon fluoride gas at 193 nm on serratia marcescens, pseudomonas aeruginosa, staphylococcus aureus, streptococcus faecalis, hemophilus influenzae, candida albicans, and aspergillus niger (collectively labeled "the microorganisms") was examined. colonies were subjected to a variable number of radiation pulses from the excimer laser applied after a 36-hour period of incubation at 37 degrees c, at which time the colonies were fully g ... | 1988 | 3194107 |
| hydrolysis of cellulose by a mixture of trichoderma reesei cellobiohydrolase and aspergillus niger endoglucanase. | two endoglucanase-containing fractions were separated from aspergillus niger cellulase by gel filtration and fast protein liquid chromatofocusing (fplc). they possessed no ability to bind to or hydrolyze insoluble microcrystalline cellulose (avicel) but were active toward soluble carboxymethylcellulose. no synergism was observed between trichoderma reesei cellobiohydrolase i and either endoglucanase from a. niger. these findings may indicate that the role of the endoglucanase component of cellul ... | 1988 | 3196759 |
| models of retinoid metabolism: microbial biotransformation of alpha-ionone and beta-ionone. | retinoic acid [1] and its analogues (retinoids) have recently generated interest as possible chemopreventive agents, but 1 is rapidly metabolized to many known as well as unknown products. recently, microbial models have been employed for the study of mammalian metabolism. in this study two fungi, aspergillus niger atcc 16888 and cunninghamella blakesleeana atcc 8688a, were found to biotransform beta-ionone [2] and alpha-ionone [8] and may serve as models for the mammalian metabolism of retinoic ... | 1988 | 3204383 |
| mycoflora and mycotoxins of peanut (arachis hypogaea l.) seeds in egypt. iii. cellulose-decomposing and mycotoxin-producing fungi. | from 40 peanut seed samples collected in egypt, forty-three species and one variety of fungi, belonging to 16 genera, were collected. the most dominant genera were aspergillus (11 species + one variety), penicillium (11 species) and fusarium (4 species). from the preceding genera a. fumigatus, a. flavus, a. niger, p. chrysogenum and f. oxysporum were the most frequent species. forty-nine isolates belonging to 12 species and one variety were tested for production of mycotoxins, after growth on li ... | 1988 | 3216881 |
| fungal infections of ear with special reference to chronic suppurative otitis media. | fungus were found to take important role in ear infections of the 344 patients (csom 286, otomycosis 44, otitis externa 14), significant fungal infections (with positive smear and culture) were detected on 49%, 79.5%, 66.6% patients respectively. 84.8% patients were detected both by smear and culture, 14.1% patients by culture and 0.1% patients in smear preparation only. in csom patients, age predominated in 20-27 yrs group, sex in male below 30 yrs, and aspergillus flavus, a. niger, penicillium ... | 1988 | 3216882 |
| genetic analysis and the construction of master strains for assignment of genes to six linkage groups in aspergillus niger. | a start has been made on establishing a collection of aspergillus niger colour and auxotrophic mutants with an isogenic background for use as a source of genetic markers. all strains have short conidiophores (csp a1), which makes them easy to handle on test plates. genetic markers were combined stepwise by somatic recombination. somatic diploids were obtained at frequencies of 10(-6) -10(-5) with conidiospores collected from a heterokaryon. the haploidization of heterozygous diploids was induced ... | 1988 | 3224384 |
| a phosphate-repressible acid phosphatase gene from aspergillus niger: its cloning, sequencing and transcriptional analysis. | the cloning and sequencing of an aspergillus niger gene encoding a secreted form of phosphate-repressible acid phosphatase by complementation of a paca (phosphate-repressible acid phosphatase) mutant of aspergillus nidulans is described. the gene contains two introns, 201 and 265 nt in length, and codes for a 1.6-kb transcript. both phosphate concentration and ph of the growth medium affect the level of expression of the gene in a. niger. similar regulation is observed in a. nidulans transforman ... | 1988 | 3224828 |
| microorganisms associated with mouldiness of dried yam chips and their prevention. | the broad objective of this study was to isolate and identify the microorganisms causing mouldiness of stored yam chips and to look for ways of preventing the problem. microorganisms isolated included aspergillus flavus, a. glaucus, a. nidulans, a. niger, a. ochraceous, a. tamarii, a. candidus, penicillium oxalicum, trichoderma longibrachyatum, rhizopus nigricans, cylindrocarpon radicicola, neurospora crassa, botryodiplodia theobromae, bacillus subtilis, bacillus cereus, erwinia carotovora and s ... | 1988 | 3231261 |
| effect of soil treatment with sewage and sludge on fungal populations. | the effect of soil treatment with sewage and sludge at three doses (1%, 10% and 20% w/w) on its fungal population was studied in vitro. the treatment of soil with sewage significantly increased the count of total fungi after 1 week by the high dose, and after 12 weeks by each of the three doses. fungi differed in their response to the different doses of sewage: some were promoted and others were inhibited. the count of total fungi was significantly raised by the low dose of sludge after 1 week o ... | 1988 | 3236218 |
| mycoflora of air-conditioners dust from riyadh, saudi arabia. | using the hair baiting technique, 6 genera and 14 species were collected on sabouraud's dextrose agar from 37 dust samples from air-conditioners. the most common fungi were chrysosporium tropicum, c. indicum, c. keratinophilum, aspergillus flavus followed by acremonium strictum and scopulariopsis brevicaulis. using the dilution-plate method, 26 genera and 52 species were collected from 37 dust samples on glucose-(23 genera and 45 species) and cellulose-(18 genera and 34 species) czapek's agar at ... | 1988 | 3236219 |
| [microbiologic transformation of flavonoids. i. flavanone derivatives]. | 1988 | 3244927 | |
| microbial models of mammalian metabolism: production of novel alpha-diketone metabolites of warfarin and phenprocoumon using aspergillus niger. | 1. the coumarin anticoagulants warfarin and phenprocoumon were metabolized by aspergillus niger via oxidative ring cleavage to yield the corresponding alpha-diketone metabolites. 2. structural identification was based upon physical, spectral, and chromatographic comparisons of isolated metabolites and synthetic standards generated by the oxidative cleavage of warfarin or phenprocoumon with pyridinium chlorochromate. 3. this pathway of metabolism has been previously observed for coumarin anticoag ... | 1988 | 3245234 |
| effect of herbicides on growth of some microscopic soil fungus species. | the effect of 14 herbicides and two herbicide combinations of three microscopic soil fungus species was investigated by miller's method. (i) none of the 14 herbicides and 2 combinations belonging to four derivative groups induced the growth of aspergillus niger, fusarium oxysporum and rhizopus nigricans; (ii) higher doses of the herbicides prevented the growth of all test organisms significantly, the triasin derivative hungazin pk being the less and tiocarbamate derivatives alirox b 80 ec, aneld ... | 1988 | 3245414 |
| [the action of dichloroacetic acid derivatives on citric acid biosynthesis in aspergillus niger]. | 1988 | 3249565 | |
| element localisation and distribution in the fungi aureobasidium pullulans and aspergillus niger, measured using the oxford scanning proton microprobe. | the distribution and relative concentrations of essential elements were determined in germ tubes of aureobasidium pullulans and hyphae of aspergillus niger using the oxford scanning proton microprobe. in both fungi, k, p and s were the major constituents, with ca, na and trace metals like cu, mn, fe and zn present at lower levels. in a. pullulans, elements were not distributed uniformly throughout the cells and, in general, the highest elemental concentrations occurred at the tip and in the olde ... | 1988 | 3251683 |
| interleukin-2 and increased natural killer activity in mice experimentally infected with aspergillus niger. | the authors studied the production and effect of interleukin 2 obtained from t lymphocytes stimulated with aspergillus niger on populations of lgl cells from the peripheral blood and spleen of infected and non-infected mice. the results show an increase in il-2 production by t lymphocytes in the spleen. in addition, the data demonstrate an increase in lgl activity in the blood and spleen of infected mice. this was seen as the percentage of growth inhibition towards aspergillus niger, around the ... | 1988 | 3265173 |
| [house dust mold in istanbul. i. detection of mold flora in bed dust]. | in our study, mycological analyses were carried out on 110 randomly chosen houses for bed dusts. 748 mould strains belonging to 117 genus and species were isolated and identified. air-borne pathogenic fungi, fungi that are causative agents of pulmonary allergy such as cladosporium spp., aspergillus glaucus group, alternaria, ulocladium, aureobasidium pullulans, aspergillus niger group were isolated. | 1988 | 3273596 |
| influence of temperature on growth rate and lag phase of fungi isolated from argentine corn. | the influence of temperature on the growth of nine strains of fungi belonging to the genera eurotium, aspergillus, penicillium and fusarium has been investigated for the temperature range 15-35 degrees c. the lag phase and the growth rate were evaluated by using a laboratory medium. the maximum growth rate for e. repens, a. wentii and p. chrysogenum was observed at about 25 degrees c, for p. citrinum near 30 degrees c and for f. semitectum and f. moniliforme between 20 and 25 degrees c. the grow ... | 1988 | 3275297 |
| metabolism of 2-oxoaldehyde in mold. purification and characterization of two methylglyoxal reductases from aspergillus niger. | two kinds of methylglyoxal reductases were purified to apparent homogeneity from aspergillus niger and designated mgr i and mgr ii. both enzymes consisted of a single polypeptide chain with a relative molecular mass of 36,000 (mgr i) and 38,000 (mgr ii). nadph was specifically required for the activities of both enzymes and km values for nadph were 54 microm (mgr i) and 6.8 microm (mgr ii). mgr i was specific to 2-oxoaldehydes [glyoxal, methylglyoxal (km = 15.4 mm) and phenylglyoxal], whereas mg ... | 1988 | 3276516 |
| monoclonal glucose-oxidase-anti-glucose-oxidase (gag) immunosandwich assay for the detection of monoclonal antibodies on routine hematological smears. | a murine monoclonal antibody specific for aspergillus niger glucose oxidase has been prepared and used in an unlabeled antibody bridge technique for the detection of monoclonal antibodies. this procedure--the monoclonal glucose oxidase anti-glucose oxidase (gag) immunosandwich assay--provides excellent immunocytochemical labeling of routine hematological films in combination with optimal preservation of cellular details. in contrast to conventional immunofluorescence procedures, routine hematolo ... | 1988 | 3281728 |
| stereochemical studies of d-glucal hydration by alpha-glucosidases and exo-alpha-glucanases: indications of plastic and conserved phases in catalysis by glycosylases. | alpha-glucosidases from aspergillus niger, pig serum, ungerminated rice, buckwheat, and sugar beet seeds (but not from brewers' yeast or honeybee) were found to catalyze the hydration of d-glucal. each reactive alpha-glucosidase, incubated with d-glucal in d2o, was shown to protonate (deuteriate) this prochiral substrate from above its re face, i.e., from a direction opposite that assumed for protonating alpha-d-glucosidic substrates. at the same time, d-glucal hydration catalyzed by three of th ... | 1988 | 3284583 |
| synthesis and microbicidal activity of n-(2-substituted) phenyl ureas and their metal complexes. | n-2-carboxyphenyl urea (cpu), n-2-hydroxyphenyl urea (hpu), and n-2-mercaptophenyl urea (mpu) and their cu(ii), ni(ii), zn(ii), co(ii), and mn(ii) complexes were synthesized and characterized by elemental analysis, molecular weight determination, molar conductance, ir and electronic spectral data, and magnetic measurements. antibacterial activity of these ligands and their metal complexes was determined on gram positive (staphylococcus aureus) and gram negative (escherichia coli) bacteria at 35 ... | 1988 | 3286821 |
| multistep reactions with immobilized microorganisms. | this review presents some examples of new semicontinuous and continuous processes for product formation and for degradation of xenobiotics with immobilized microorganisms. a semicontinuous process for glycerol formation with saccharomyces cerevisiae cells, adsorbed on sintered glass, and a continuous production of citric acid with aspergillus niger, entrapped in calcium alginate, are given as examples of the production of primary metabolites. the production of ergot alkaloids with entrapped clav ... | 1988 | 3289565 |
| steric course of the hydration of d-gluco-octenitol catalyzed by alpha-glucosidases and by trehalase. | crystalline aspergillus niger alpha-glucosidase and highly purified preparations of rice alpha-glucosidase ii and trichoderma reesei trehalase were found to catalyze the hydration of [2-(2)h]-d-gluco-octenitol, i.e., (z)-3,7-anhydro-1,2-dideoxy-[2-2h]-d-gluco-oct-2-enitol, to yield 1,2-dideoxy-[2-2h]-d-gluco-octulose. in each case, the stereochemistry of the reaction was elucidated by examining the newly formed centers of asymmetry at c-2 and c-3 of the hydration product. the c-1 to c-3 fragment ... | 1988 | 3289613 |
| factors affecting protoplast release from some mesophilic, thermophilic and thermotolerant species of filamentous fungi using novozym 234. | factors affecting the release of protoplasts from mycelia of aspergillus fumigatus, aspergillus niger, penicillium ochrochloron and geosmithia emersonii were investigated. protoplast yields were found to be greatly dependent on the growth temperature, incubation conditions, lytic enzyme system, mycelial age and mycelial concentration. high yields of protoplasts were obtained from all four organisms after a 3 h incubation with 1 mg/ml novozym 234 in 0.1 m 2-n-[morpholino] ethane sulphonic acid (m ... | 1988 | 3292876 |
| sporicidal activity of local anaesthetics and their binary combinations with preservatives. | the sporicidal activity of a 1% solution of five local anaesthetics and five preservatives (cetrimide, chlorocresol, chlorhexidine, phenoxyethanol and phenylmercuric nitrate) at their commonly used concentrations, alone and in binary combinations, was determined against bacillus subtilis and aspergillus niger spores at different temperature levels by surface viable count technique. the sporicidal activity of all tested systems was temperature dependent and a. niger spores were much more sensitiv ... | 1988 | 3148619 |
| prevalence of airborne aspergillus flavus in khartoum (sudan) airspora with reference to dusty weather and inoculum survival in simulated summer conditions. | khartoum air was scanned for airborne aspergillus flavus for 12 months using the horizontal gravitational settling method. frequency of occurrence was related to total fungal catch and dusty weather. the aspergilli were prevalent (68% of total isolated/plate/month) and a. flavus constituted 31% of the total aspergilli. in june (hot, dry & dusty) aspergilli constituted 79% of the total isolates, whilst a. flavus represented 30% from amongst the other aspergilli. a. flavus, a. niger, a. nidulans ( ... | 1988 | 3148861 |
| microbial conversion of alpha-ionone, alpha-methylionone, and alpha-isomethylionone. | alpha-ionone, alpha-methylionone, and alpha-isomethylionone were converted by aspergillus niger jts 191. the individual bioconversion products from alpha-ionone were isolated and identified by spectrometry and organic synthesis. the major products were cis-3-hydroxy-alpha-ionone, trans-3-hydroxy-alpha-ionone, and 3-oxo-alpha-ionone. 2,3-dehydro-alpha-ionone, 3,4-dehydro-beta-ionone, and 1-(6,6-dimethyl-2-methylene-3-cyclohexenyl)-buten-3-one were also identified. analogous bioconversion products ... | 1988 | 16347747 |
| chemical stabilization of glucoamylase from aspergillus niger against thermal inactivation. | the applicability of crosslinking an enzyme to an oxidized polysaccharide by reductive alkylation to enhance thermostability has been investigated for glucoamylase from aspergillus niger. direct covalent coupling of the enzyme to periodate-oxidized dextran in the presence of nabh(3)cn results in a conjugate which has thermal properties similar to those of the native enzyme. our working hypothesis postulates that enhancement of thermostability will result from rigidification of the protein's conf ... | 1988 | 18584602 |
| aerobic fungal cell immobilization in a dual hollow-fiber bioreactor: continuous production of a citric acid. | aspergillus niger b60 was immobilized in a dual hollow-fiber bioreactor (dhfbr) to produce citric acid continuously. the fungi proliferated well in the interstitial region formed by a parallel arrangement of three microporous polypropylene hollow fibers contained within a silicone tube. long-term operation with nitrogen-enriched medium was not possible due to expansion of the silicone tubes by continual cell growth. the fungal growth could be controlled by supplying a nitrogen-deficient medium a ... | 1988 | 18584736 |
| application of fed-batch culture to citric acid production by aspergillus niger: the effects of dilution rate and dissolved oxygen tension. | studies in conventional batch culture confirmed that the maximum citric acid production rate occurred prior to exhaustion of the growth-limiting nutrient, i.e., when the growth rate was nonzero. the effects of dilution rate and the culture dissolved oxygen tension (dot) were studied in chemostat culture. maximum citric acid yield and production rate were observed at low dilution rate (0.017 h(-1)) and high dot value (90% of saturation). these findings were applied to a nitrogen-limited fed batch ... | 1988 | 18584738 |
| the enzymatic hydrolysis and fermentation of pretreated wheat straw to ethanol. | autohydrolysis and ethanol-alkali pulping were used as pretreatment methods of wheat straw for its subsequent saccharification by trichoderma reesei cellulase. the basic hydrolysis parameters, i.e., reaction time, ph, temperature, and enzyme and substrate concentration, were optimized to maximize sugar yields from ethanol-alkali modified straw. thus, a 93% conversion of 2.5% straw material to sugar syrup containing 73% glucose was reached in 48 h using 40 filter paper units/g hydrolyzed substrat ... | 1988 | 18587784 |
| morphology and growth of aspergillus niger atcc 26036 cultivated at several shear rates. | 1988 | 18587793 | |
| the effect of zeolite on the toxicity of lead to fungi. | in order to determine whether clinoptilolite, a naturally occurring zeolite, had any ameliorative effect on lead (pb) toxicity to fungi, a series of growth experiments were performed. three fungi, aspergillus niger, botrytis cinerea, and fusarium culmorum, were grown on appropriately amended solid agar media, and their linear extension rates determined. b. cinerea was 25% inhibited, as compared to a control, at 100 mg dm(-3) pb, and completely inhibited at 1000 mg dm(-3) pb. f. culmorum was comp ... | 1988 | 15092664 |
| decree no. 88-058/pcms/m/mj of 18 february 1988 amending article 12 of decree no. 84-132/pcms/mj of 23 august 1984 applying ordinance no. 84-33 of 23 august 1984. | this decree sets forth amended rules on the documents to be submitted by the foreign wife of a citizen of niger who wishes to obtain niger citizenship by reason of her marriage. it also provides that the documents are to be sent to the ministry of justice within 3 months of her declaration of intent to become a niger citizen. | 1989 | 12344212 |
| immobilization of endo-polygalacturonase from aspergillus niger on various types of macromolecular supports. | endo-polygalacturonase (endo-pg) was immobilized on a wide range of natural and synthetic macromolecular supports and their modified derivatives representing many chemical classes, including esters, amides, phenols, alkyl- and arylamines, and carboxyl derivatives. the immobilization entailed methods of adsorption alone as well as covalent bond formation using glutaraldehyde or carbodiimide or via the diazo-coupling reaction. the most promising system proved to be immobilization on trimalehylchit ... | 1989 | 18587858 |
| evidence for nitrogen catabolite repression during citric acid production by aspergillus niger under phosphate-limited growth conditions. | experiments performed in batch fermentation under phosphate-limited growth conditions showed that the citric acid yield was inversely related to the excess nitrogen concentration in the medium. results from chemostat culture confirmed a negative relationship between the citric acid yield and both the specific growth rate and the nitrogen consumption rate. this is evidence for nitrogen catabolite repression. a fed-batch fermentation performed under dual phosphate/nitrogen limitation produced resu ... | 1989 | 18587892 |
| effects of temperature on lactose hydrolysis by immobilized beta-galactosidase in plug-flow reactor. | the hydrolysis of lactose using immobilized beta-galactosidase (from aspergillus niger) on phenol-formaldehyde resin was studied at temperatures between 8 and 60 degrees c and initial lactose concentrations ranging from 2.5 to 20.0%. a model involving enzyme-galactose complex similar to michaelis-menten kinetics with competitive product (galactose) inhibition is suitable to describe the lactose hydrolysis reaction. a small degree of lack of fit between the model and the data was found to be due ... | 1989 | 18587995 |
| microbial conversion of beta-ionone by immobilized aspergillus niger in the presence of an organic solvent. | aspergillus niger jts191 was capable of the conversion of beta-ionone to a mixture of its derivatives that is utilized to an essential oil of tobacco. the authors attempted this microbial conversion in the presence of an organic solvent to improve its reaction rate. the addition of isooctane accelerated the microbial conversion of beta-ionone. it took three days to complete the reaction whereas without isooctane it took more than six days. the addition of isooctane also improved the resistance o ... | 1989 | 18588037 |
| subsite mapping of aspergillus niger glucoamylases i and ii with malto- and isomaltooligosaccharides. | glucoamylase, industrially derived from aspergillus niger, was chromatographically separated into forms i and ii and purified to near homogeneity. preparations were proved to be free of d-glucosyltransferase by electrophoretic and differential inhibition tests. maximum rates and michaelis constants were obtained for both glucoamylases i and ii with maltooligosaccharides from maltose to maltoheptaose and with isomaltooligosaccharides from isomaltose to isomaltohexaose. subsite maps were calculate ... | 1989 | 18588152 |
| kinetics of the hydrolysis of di- and trisaccharides with aspergillus niger glucoamylases i and ii. | near-homogeneous forms of glucoamylases i and ii, previously purified from an industrial aspergillus niger preparation, were used to hydrolyze a number of di- and trisaccharides linked by alpha-d-glucosidic bonds. maximum rates and michaelis constants were obtained at various temperatures and ph values with glucoamylase i for the disaccharides beta,alpha-trehalose, kojibiose, nigerose, maltose, and isomaltose and the trisaccharides panose and iso-maltotriose, and with glucoamylase ii for maltose ... | 1989 | 18588153 |
| kinetics, equilibria, and modeling of the formation of oligosaccharides from d-glucose with aspergillus niger glucoamylases i and ii. | near-homogeneous forms of glucoamylases i and ii, previously purified from an industrial aspergillus niger preparation, were incubated with d-glucose at a number of temperatures and ph values. kinetics and equilibria of the formation of alpha,beta-trehalose, kojibiose, nigerose, maltose, isomaltose, panose, and isomaltotriose, which with isomaltotetraose were the only products formed, were determined. there was no difference in the abilities of ga i and ga ii to form these products. activation e ... | 1989 | 18588154 |
| hydrodynamics and mass transfer in aspergillus niger fermentations in bubble column and loop bioreactors. | the influence of aspergillus niger broth rheology, bioreactor geometry, and superficial gas velocity on the volumetric liquid phase oxygen transfer coefficient (k(l)a(l)), riser gas holdup (epsilon(gr)), and circulating liquid velocity (u(lr)) was studied in a bubble column (bc) and two external-circulation-loop airlift (eclal) bioreactors. the results are compared to those of previous studies on homogeneous fluids and in particular with a recent study on non-newtonian carboxymethylcellulose (cm ... | 1989 | 18588159 |
| a new graphical method for determining parameters in michaelis-menten-type kinetics for enzymatic lactose hydrolysis. | a new graphical method was developed to determine the kinetic parameters in the michaelis-menten-type equation. this method was then applied to studying the kinetics of lactose hydrolysis by aspergillus niger beta-galactosidase. in this study, the reaction temperature ranged between 8 and 60 degrees c, and the initial lactose concentration ranged between 2.5 and 20%. a kinetic model similar to the conventional michaelis-menten equation with competitive product inhibition by galactose was tested ... | 1989 | 18588163 |
| extraction of zinc from industrial waste by a penicillium sp. | zinc was extracted from a filter residue of a copper works (58.6% zinc) by a penicillium sp. isolated from a metal-containing location. by isotachophoresis citric acid was identified as the leaching agent. citrate was only formed when the leaching substrate was present. this production of citrate was different in several ways from that achieved by aspergillus niger: glucose was utilized before fructose; the initial concentration of zinc was 50 to 500 times higher than usual in citrate fermentati ... | 1989 | 16347908 |
| changes in the specificity of blood groups induced by enzymes from soil fungi. | three strains of cunninghamella elegance (c. elegance), penicillium simplicissimum (p. simpl.), and aspergillus niger (a. niger) were isolated from soil that produced the enzymes acting on blood groups a and b red cells. culture filtrates from these fungi contained alpha-n-acetyl-d-galactosaminidase as an a-decomposing enzyme, which resulted in an almost complete loss of a specificity and an enhancement of blood group h activity as measured by elution tests using monoclonal antibodies. they also ... | 1989 | 2918293 |
| seminal vesicle and coagulating gland growth induced by intraperitoneal inoculation of fungi in mice. | the effect of fungi on the growth of body organs in mice was investigated. single, intraperitoneal injections of yeasts (cryptococcus albidus, saccharomyces cerevisiae, schizosaccharomyces octosporus) or molds (aspergillus niger, geotrichum candidum, mucor haemalis) induced an increase in the mass of seminal vesicles and coagulating glands independent of whole body weight changes in mice. | 1989 | 2919824 |
| fungi in two swimming pools in assiut town, egypt. | 50 water samples from 2 public swimming pools were tested for the presence of fungi using the hair baiting technique and plate count methods. sabouraud's-dextrose agar and littman-oxgall agar media were used for isolation of the fungi. of the keratinolytic fungi 8 species were recorded of which three species were dermatophytes (trichophyton terrestre, 14% of the samples; t. mentagrophytes, 10% and microsporum gypseum, 6%). on littman-oxgall agar the following species were recovered: aspergillus ... | 1989 | 2475993 |
| fungi on the claws of buffalo and cow in egypt. | the frequency of occurrence of fungi in 120 claw samples of buffalo and cow from different localities at assiut governorate was determined. thirty-seven species which belong to twenty genera were collected from the two substrates and the most common genera were chrysosporium (eleven species of chrysosporium were isolated). aspergillus and scopulariopsis. from the preceding genera chrysosporium sulfureum was common on cow claws but c. merdarium was slightly dominant on buffalo claws than on cow c ... | 1989 | 2482351 |
| [in vitro study of the antifungal activity of two chlorine derivatives to be used in antisepsis]. | the activity of two chlorine derivates, sodium hypochlorite in water solution with nacl (product a) and electrolytic chloroxidant (product b) has been tested in vitro against potentially human pathogenic fungi (aspergillus niger, aspergillus fumigatus, microsporum gypseum, candida albicans, cryptococcus neoformans, trichophyton mentagrophytes, microsporum canis, epidermophyton floccosum, trichophyton rubrum, sporotrix schenkii). for a. niger, the relation of the two compounds has also been consi ... | 1989 | 2483652 |
| isolation of human pathogenic fungi from barbers' shop of chhindwara (mp): a preliminary study. | isolation of human pathogenic fungi was made from the barbers' shops of chhindwara. trichophyton mentagrophytes showed highest percentage of occurrence (30%), followed by trichophyton rubrum (26%) and aspergillus fumigatus (18%) whereas, the lowest relative density was exhibited by aspergillus niger and a hyphomycetous fungus (2%). | 1989 | 2486270 |
| effect of antibiotics in controlling the production of cell wall degrading enzymes in vitro and disease development in vivo. | production of cellulolytic, proteolytic and pectolytic enzymes by all the three isolates of a. niger was inhibited by all the tested antibiotics. nystatin (500 ppm) could cause complete inhibition of pg enzyme production, whereas pmg production was checked by amoxicillin, nystatin, chloramphenicol and raficillin at 100 ppm concentration. all the antibiotics at 500 ppm concentration checked the cellulase production in all the three isolates of a. niger. nystatin and raficillin (100 ppm) completel ... | 1989 | 2486272 |
| thermodynamics of the interaction of sodium-n-dodecyl sulphate with aspergillus niger catalase in high ionic strength aqueous solutions. | the thermodynamic parameters for the interaction between sodium n-dodecyl sulphate (sds) and aspergillus niger catalase in aqueous solution at ph 3.2 and 6.4 have been measured by microcalorimetry and equilibrium dialysis over a range of ionic strength from 0.05 to 0.2 at 25 degrees c. binding isotherms have been interpreted in terms of theoretical models (hill equation and wyman binding potential). the gibbs energies of interaction become increasingly negative with increase in ionic strength an ... | 1989 | 2489055 |
| [otomycosis: presentation of 15 cases]. | fifteen cases of otomycosis in 11 women and 4 men attending the clinic hospital in salamanca during the period from 1984 to 1988 are discussed. a. niger was the species isolated on the majority of occasions (73.3%). the commonest form of presentation of otomycosis was simple chronic (73.3%). the clinical pictures was characterized by serous secretion and pruritus. otomycosis with associated bacterial infection (20.0%) was the cause of the most intense symptoms, featuring suppuration and hypoacus ... | 1989 | 2490720 |
| mechanism and binding specificity of beta-glucosidase-catalyzed hydrolysis of cellobiose analogues studied by competition enzyme kinetics monitored by 1h-nmr spectroscopy. | the application of high-resolution 1h-nmr spectroscopy to monitor substrate and product time dependencies in progress curve enzyme kinetics is described with beta-glucosidase-catalyzed hydrolyses of cellobiose analogues as examples. it is demonstrated that inhibition patterns, relative binding specificities and catalytic rates can be inferred from competition experiments with two or more substrates. it could be concluded from competition experiments that substrates which form less stable enzyme- ... | 1989 | 2492229 |
| indolinone derivatives as potential antimicrobial agents. | 1-substituted aminomethyl-3-cyclohexylthiosemicarbazone-2-indolinones (i) were tested for their antibacterial activity against bacillus pumilis, bacillus brevis and bacillus megaterium and antifungal activity against aspergillus flavus, aspergillus fumigatus and aspergillus niger. the majority of the compounds were found to exhibit promising antibacterial and antifungal activities. these compounds were also screened for their antiviral action against tobacco mosaic virus in nicotiana glutinosa p ... | 1989 | 2501946 |
| immunofluorescence staining of hyphae in the histopathological diagnosis of mycoses in cattle. | an immunofluorescence staining system was developed for the identification of mycelial fungi causing systemic mycoses in cattle. antisera to absidia corymbifera, aspergillus fumigatus, aspergillus flavus, and aspergillus niger were raised in rabbits. tissue sections of experimentally infected mice were used to determine the specificity of immune staining with antisera that had been absorbed with heterologous antigens. when 46 lesions from 22 cattle with systemic mycosis were studied, the infecti ... | 1989 | 2502614 |
| etiological agents of otomycosis in nigeria. | a total of 159 suspected cases of otomycosis comprising 101 adults and 58 children, 61 males and 98 females were investigated. of these, 36 cases were confirmed specifically of mycotic etiology on the basis of microscopic demonstration of fungal structures in epithelial debris/plugs and positive culture. another 31 cases positive for fungi by culture but negative for direct microscopy were considered of doubtful fungal etiology. the predominant etiological agents in the confirmed cases were aspe ... | 1989 | 2503721 |
| expression of the escherichia coli beta-glucuronidase gene in industrial and phytopathogenic filamentous fungi. | a chimaeric beta-glucuronidase (gus) gene has been created by ligating the aspergillus nidulans glyceraldehyde 3-phosphate dehydrogenase promoter to the coding sequence of the e. coli uida gene. co-transformation of this vector into a. nidulans, a. niger and the tomato pathogen fulvia fulva (syn. cladosporium fulvum (cooke] resulted in the expression of beta-glucuronidase. gus activity was detected by growth on agar media containing x-gluc and by enzyme assays of mycelial extracts. expression of ... | 1989 | 2504501 |
| covalently bound phosphate residues in bovine milk xanthine oxidase and in glucose oxidase from aspergillus niger: a reevaluation. | the reported presence of covalently bound phosphate residues in flavoproteins has significant implications with regard to the catalytic mechanisms and structural stability of the specific enzymes themselves and in terms of general cellular metabolic regulation. these considerations have led to a reevaluation of the presence of covalently bound phosphorus in the flavoproteins xanthine oxidase (xanthine: oxygen oxidoreductase, ec 1.1.3.22) and glucose oxidase (beta-d-glucose: oxygen 1-oxidoreducta ... | 1989 | 2505251 |
| comparison of immunodiffusion and enzyme linked immunosorbent assay for antibodies to four aspergillus species. | antigenic extracts were prepared from aspergillus fumigatus, a niger, a flavus and a terreus for use in enzyme linked immunosorbent assay (elisa) and immunodiffusion (id) tests for aspergillus antibodies to determine whether the use of antigenic extracts from species other than a fumigatus increased the sensitivity of the elisa. elisa titres correlated well with positive id tests. patient titres by elisa were significantly higher than control titres for all species. patient titres to a niger wer ... | 1989 | 2511230 |
| effect of deglycosylation of n-linked sugar chains on glucose oxidase from aspergillus niger. | endo-beta-n-acetylglucosaminidase from flavobacterium sp. released about 30% of the n-linked sugar chains from the glucose oxidase of aspergillus niger. to elucidate the role of the carbohydrate moiety, the enzymatic properties of native and carbohydrate-depleted glucose oxidases were compared. it was found that their catalytic activities and thermal and ph stabilities were identical. however, the carbohydrate-depleted glucose oxidase was more rapidly precipitated by the addition of trichloroace ... | 1989 | 2511903 |
| synergistic antifungal action of 8-quinolinol and its bischelate with copper(ii) and with mixed ligand chelates composed of copper(ii), 8-quinolinol, and aromatic hydroxy acids. | antifungal studies were made of mixtures of minimal inhibitory concentrations (mics) of 8-quinolinol and its bischelates with copper(ii), zinc(ii), and manganese(ii) and with mixed ligand chelates composed of 8-quinolinol, copper(ii) and a second ligand including salicylic acid, 3-hydroxy-2-naphthoic acid, 3,5-diiodosalicylic acid, and 4-bromo-3-hydroxy-2-naphthoic acid. mixtures of the mics of the bischelates of 8-quinolinol with copper(ii) and zinc(ii) and copper(ii) and manganese(ii), as well ... | 1989 | 2516124 |
| side chain reactivities of glucoamylase g2 from aspergillus niger evaluated by group-specific chemical modifications. | treatment of glucoamylase g2 with large excesses of different group specific reagents resulted in modification of 25% of the histidyl, 15% of the tyrosyl, 20-40% of the arginyl, 30-50% of the lysyl and none of the methionyl residues. the modified groups were not critical since the various derivatives possessed from 50% to 100% residual enzymatic activity and retained the thermostability. carboxamidomethylation occurred specifically at his254 with essentially no change of the kinetic parameters f ... | 1989 | 2516722 |
| amino acid sequence of cytochrome c from aspergillus niger. | cytochrome c from aspergillus niger consists of two forms, a major one (80%) with 111 amino acid residues and a minor one (20%) with 108 residues, missing the three n-terminal residues of the major one. the primary sequence of a. niger cytochrome c was determined by standard spinning-cup edman degradation of purified peptides and of pairs of peptides, from which the desired sequence was readily deduced by subtraction of common sequencies. except for the extension and some variability at the n-te ... | 1989 | 2544196 |
| the relationship between the glucose oxidase subunit structure and its thermostability. | the thermostability of glucose oxidase (beta-d-glucose: oxygen 1-oxidoreductase, ec 1.1.3.4) at 60 degrees c has been studied as a function of its concentration in various media (pure water and pure deuterium oxide). in deuterium oxide, glucose oxidase is more stable than in water, and two kinds of stabilizing effect have been observed: the medium-organization effect and the enzyme-concentration effect. this effect has been related to the glucose oxidase subunit structure. this enzyme contains f ... | 1989 | 2553126 |
| cloning of a new bidirectionally selectable marker for aspergillus strains. | mutants that lack adenosine triphosphate sulfurylase (atpsase; ec 2.7.7.4) are unable to use sulfate as sole source of sulfur and are also resistant to selenate. these mutants, denoted sc-, are readily obtained from any strain of aspergillus niger or aspergillus nidulans by the strong selection for selenate resistance. we have cloned the gene encoding atpsase from a. nidulans by complementation of an sc mutant strain of a. nidulans with a gene library and show that plasmids containing this gene ... | 1989 | 2558969 |
| the oxidative part of the glucose-oxidase reaction. | 1. kinetic parameters of the oxidative part of glucose-oxidase reaction have been measured with 16 different electron-acceptors and glucose as a substrate. 2. in each case, the rate-limiting portion of the oxidative part of reaction was the formation of the e-fadh2.acceptor-complex; this rate was ph-independent around the ph-optimum of the enzyme. 3. in each case, e-fadh2 acceptor-complex was undetectable in the steady-state kinetics, with the exception of cytochrome-c. 4. the rates of redox rea ... | 1989 | 2583344 |
| rhizomucor miehei triglyceride lipase is processed and secreted from transformed aspergillus oryzae. | the cdna encoding the precursor of the rhizomucor miehei triglyceride lipase was inserted in an aspergillus oryzae expression vector. in this vector the expression of the lipase cdna is under control of the aspergillus oryzae alpha-amylase gene promoter and the aspergillus niger glucoamylase gene terminator. the recombinant plasmid was introduced into aspergillus oryzae, and transformed colonies were selected and screened for lipase expression. lipase-positive transformants were grown in a small ... | 1989 | 2586234 |
| [pharmacokinetic studies of the activity of a ph-labile alpha-l-arabinofuranosidase following i.v. injection into tumor-bearing mice]. | in order to increase the selectivity of tumor chemotherapy, graffi et al. have proposed the application of xenogenic enzymes, which are able to split transport forms of carcinostatics under the ph-conditions in the tumor more vigorously than in the normal tissues. this paper describes the distribution within the body and elimination of the activity of the ph-labile alpha-l-arabinofuranosidase from g. myabena compared with the ph-stable enzyme from a. niger, using tumor bearing mice. in vitro, th ... | 1989 | 2589931 |
| enzyme catalysed non-oxidative decarboxylation of aromatic acids. ii. identification of active site residues of 2,3-dihydroxybenzoic acid decarboxylase from aspergillus niger. | in order to understand the mechanism of decarboxylation by 2,3-dihydroxybenzoic acid decarboxylase, chemical modification studies were carried out. specific modification of the amino acid residues with diethylpyrocarbonate, n-bromosuccinimide and n-ethylmaleiimide revealed that at least one residue each of histidine, tryptophan and cysteine were essential for the activity. various substrate analogs which were potential inhibitors significantly protected the enzyme against inactivation. the modif ... | 1989 | 2590221 |