Publications

an inquiry into the adoption of the term mycobacterium hansenii.	an inquiry was made among various organs of the public health service of the state of s. paulo, brazil, members of the scientific council and editorial board of "hansenologia internationalis", and professors of dermatology, bacteriology, neurology, pathology and preventive medicine of brazil, having in view the possibility of using the term mycobacterium hansenii in that periodical, as a substitute for mycobacterium leprae. the results were considered favorable to the change and led to the adopt ...	1981	6749680
intra-neural ceroid-like pigment following the treatment of lepromatous leprosy with clofazimine (b663; lamprene).	a 33 year old male nigerian presented with widespread involvement of peripheral nerves, several of which were tender and painful. nerve biopsies confirmed the presence of mycobacterium leprae in both endoneurial and perineurial areas, mainly in foamy macrophages (virchow cells), but there were also large accumulations of an amorphous, acid-fast and alcohol-fast material which was not obviously of bacterial origin. appropriate stains indicated that this had many characteristics of lipofuscin. alt ...	1981	6260900
demonstration of antibodies reacting with different determinants on mycobacterium leprae antigen 7.	a purified preparation of m. leprae antigen 7 was used to investigate the specificity of anti-m. leprae 7 antibodies in leprosy sera in a radioimmunoassay. a solution containing arabinogalactan (ag) and arabinomannan (am) inhibited the antibodies in some sera to a great extent, whereas the antibody activity was virtually unchanged in other sera under the same conditions. these findings indicate that the antibodies are directed against different determinants on m. leprae antigen 7. antibodies aga ...	1981	6168595
evaluation of fluorescent microscopy for detection of mycobacterium leprae.		1981	6166792
antigen-specific suppressor cells in subclinical leprosy infection.	a two-stage in-vitro culture system was used to assay cells which suppress the lympho-proliferative response to mycobacterium leprae (ml). responses to ml, purified protein derivative of tuberculin, and streptokinase-streptodornase were preferentially suppressed by mitomycin-treated cells which had been primed with the same antigen in a 7-day primary culture. healthy subjects exposed to leprosy for more than 3 years showed strong suppression of the response to ml antigens (11 of 12 showed more t ...	1981	6171698
comparison of 22 species of mycobacteria by immunodiffusion against an absorbed reference leprosy serum.	lithium acetate antigenic extracts of 22 species of acetone-treated mycobacteria were tested by immunodiffusion precipitation for reactivity with a pool of sera from treated lepromatous leprosy patients (arls). this arls had been adsorbed with m. bovis (bcg, m. vaccae, cardiolipin, and lecithin to make it specific for m. leprae when used in an indirect immunofluorescence test. the arls produced two precipitin lines with m. leprae extract, one of which formed a line of identity with extracts of m ...	1981	6172393
do anti-leprosy drugs reach mycobacterium leprae in peripheral nerves?		1981	6110969
occurrence of gamma-glutamyl transpeptidase activity in several mycobacteria including mycobacterium leprae.	gamma-glutamyl transpeptidase (gamma-gt) activity, which catalyzes the transfer of the "gamma-glutamyl" group of gamma-glutamyl compounds to several dipeptide and amino acid acceptors, was found to be present in several mycobacteria, including m. leprae, both in cell suspensions and in cell-free sonicates. glycyl d-amino acids were active as acceptors, particularly glycyl-d-alanine and alpha, epsilon-diaminopimelic acid, among the amino acids. two mycobacterial isolates obtained from biopsy mate ...	1981	6114046
studies on the defect in cell-mediated immunity in lepromatous leprosy using hla-d-identical siblings. absence of circulating suppressor cells and evidence that the defect is in the t-lymphocyte, rather than the monocyte, population.	sixteen healthy siblings were identified as hla-d-identical to 12 borderline lepromatous or polar lepromatous leprosy patients by the absence of a mixed lymphocyte reaction (mlr). the peripheral blood mononuclear cells (pbm) of the healthy siblings showed a lymphoproliferative response (delta cpm) to mycobacterium leprae antigens which was about fivefold or more greater than that of the lepromatous patients. lepromatous pbm, with or without mitomycin c treatment, were co-cultured with a constant ...	1982	6176016
production and partial characterization of monoclonal antibodies to mycobacterium leprae.	monoclonal antibodies to mycobacterium leprae were produced by the fusion of balb/c splenocytes and lymph node cells to balb/c myeloma (nsi/1) cells. eleven monoclonal antibodies were characterized as to their reactivity with m. leprae and 18 other mycobacterial species by enzyme-linked immunosorbent assay and immunofluorescence. two monoclonal antibodies reacted only with m. leprae, and the other nine showed unique patterns of reactivity by enzyme-linked immunosorbent assay. one monoclonal anti ...	1982	6179876
comparative ultrastructure of mycobacterium leprae and m. avium grown in experimental hosts.		1982	6180669
taxonomy of mycobacterium leprae and m. lepraemurium.		1982	6180670
a fluorescent staining procedure for determining the viability of mycobacterial cells.	a fluorescent staining procedure has been developed which rapidly, accurately, and economically measures the viability of mycobacterial cells. m. smegmatis and m. phlei have served as prototype organisms to establish conditions which ensure optimal staining. the staining method incorporates the use of the fatty acid ester fluorescein diacetate (fda) and ethidium bromide (eb). non-polar, non-fluorescent fda enters live cells where it is enzymatically hydrolyzed by acetylesterase to polar, fluores ...	1982	6180992
in vitro stimulation of lymphocytes in leprosy patients, healthy contacts of leprosy patients, and subjects not exposed to leprosy. comparison of an antigen fraction prepared from mycobacterium leprae and tuberculin-purified protein derivative.	in vitro lymphocyte stimulation was performed on peripheral blood lymphocytes from 48 leprosy patients, 15 healthy contacts of leprosy patients, and 16 normal controls who lived in a leprosy-free area and who had not been exposed to leprosy. tuberculin ppd and an antigen fraction. mlw 1, prepared from m. leprae, were used as stimulants. the mlw 1 preparation contained one antibody-precipitable component when tested in crossed immunoelectrophoresis against a polyvalent anti-m. leprae immunoglobul ...	1982	6182610
t-cell-dependent polyclonal activation by soluble mycobacterial extracts of b cells in peripheral blood mononuclear cell populations from leprosy patients and normal donors.	soluble extracts of ultrasonically disrupted mycobacteria (including mycobacterium leprae) cause strong t-cell-dependent, cyclosporin a-sensitive, polyclonal b-cell activation, in human peripheral blood mononuclear cell preparations. it is not enhanced by cortisol. cells from cases of lepromatous leprosy show no polyclonal b-cell activation in the presence of extracts of m. leprae, although they respond normally to other mycobacterial species. the polyclonal b-cell activation response to the m. ...	1982	6183191
sds-page analysis of m. leprae protein antigens reacting with antibodies from sera from lepromatous patients and infected armadillos.	studies have been conducted to characterize m. leprae bacilli derived from infected armadillos. first, the proteins of the mycobacterial extracts were fractionated by sds-page. subsequently, the proteins in the gel were electrophoretically transferred on a strip of nitrocellulose paper by the technique of 'electrophoretic blotting'. the separated bacterial protein bands, thus immobilized on the nitrocellulose paper were made to react immunologically with sera from the lepromatous patients, infec ...	1982	6184189
structure and antigenicity of the major specific glycolipid antigen of mycobacterium leprae.	earlier we reported on the presence of a specific phenolic glycolipid (phenolic glycolipid-i) in mycobacterium leprae, and in infected armadillo tissues (hunter, s. w., and brennan, p. j. (1981) j. bacteriol. 147, 728-735). it had an inherent oligosaccharide, composed of 3-o-me-rhamnose, 2,3-di-o-me-rhamnose, and 3,6-di-o-me-glucose, glycosidically linked to the phenol substituent. the structure of the oligosaccharide has now been determined, by partial acid hydrolysis, permethylation, 1h nmr, a ...	1982	6184369
lack of correlation between morphological index and viability as assessed by the uptake of 3h-thymidine by macrophage resident m. leprae.	thirty strains of m. leprae derived from skin biopsies of lepromatous leprosy patients were scored for morphological index (mi) and concurrently maintained for 2 weeks in macrophage cultures containing 3h thymidine. selective and significant incorporation of the radioactive label was observed in cultures containing freshly extracted m. leprae as compared to control cultures with autoclaved bacilli from the same biopsy. the percentage incorporation of 3h thymidine ranged from 103 to 1140%. morpho ...	1982	6184544
effect of purified protein derivative and sonicates of mycobacterium leprae and mycobacterium bovis bcg on thromboplastin response in human monocytes in vitro.	human monocytes isolated from peripheral blood responded with increased thromboplastin expression upon stimulation in vitro with three mycobacterial antigens: tuberculin purified protein derivative and sonicates of mycobacterium bovis bcg and mycobacterium leprae. the stimulating principle of mycobacteria is probably a cell wall constituent since crude extracts of cell walls were 2.5 to 25 times more potent in stimulating thromboplastin synthesis than were whole sonicates. this thromboplastin re ...	1982	6185426
nerve abscess in lepromatous leprosy.		1982	6285081
[modification of the basic stain of the ziehl-neelsen technic in the identification of mycobacterium leprae].	authors analyse the counterstain with methylene blue solution in the usual ziehl-neelsen method. considerations are made on the characteristics of the dye substance. a technique alteration is proposed: a "concomitant alkalization" of the classic aqueous methylene blue solution, by adding some drops of sodium hydroxide solution, 1:500, on the slide at the moment of the staining. by this technique it was observed: a) a larger validity period of the solution; b) an absence of precipitate in the sli ...	1982	6198275
composite skin contact smears in multibacillary leprosy patients.	"composite skin contact smears" technique was used in 20 multibacillary leprosy patients to find out the presence of afb on their intact skin. one hundred and twenty one afb were found in 930 sq.cm. area of skin surface examined. seventy-five afb were solidly staining and 46 were granular. the bacilli were found in all the sites examined. immunofluorescent staining showed m. leprae in one out of four patients. the number of bacilli discharged from the intact skin seems adequate for the transmiss ...	1982	6191122
treatment of sulphone resistant leprosy--a review of sixty one cases.		1982	6191123
defect in the generation of cytotoxic t cells in lepromatous leprosy.	cytotoxic t cells are consistently produced in normal individuals after in vitro stimulation by a pool of mitomycin-treated normal lymphocytes. patients suffering from lepromatous leprosy (ll), presenting with large amounts of mycobacterium leprae and without a history of erythema nodosum leprosum (enl) are unable to generate such cytotoxic t cells, while lepromatous patients with enl which, in the present study were all deprived of m. leprae, react normally.	1982	6214342
imbalances in t cell subpopulations in lepromatous leprosy.	circulating t cells, t helper, and t suppressor cells were investigated in 24 lepromatous patients, using murine hybridoma-derived monoclonal antibodies okt 3, okt 4, and okt 8. six bacillary lepromatous patients without recent enl were studied; in this group, suppressor cells were increased and helper cells diminished, resulting in a decrease in the helper/suppressor (h/s) ratio. nine bacteriologically negative lepromatous patients without recent enl were studied. t cell subsets distribution wa ...	1982	6216219
the cutaneous infiltrates of leprosy: cellular characteristics and the predominant t-cell phenotypes.	we report on the characteristics of cells in the cutaneous lesions and blood of 21 patients with lepromatous, tuberculoid, and intermediate forms of leprosy. a large proportion of the infiltrates in lepromatous lesions consist of macrophages heavily parasitized with mycobacterium leprae. the t cells in the lesions are devoid of okt4/leu 3a-positive ("helper") cells and consist almost exclusively of okt8/leu 2a-positive ("suppressor") populations. in contrast, the tuberculoid infiltrates contain ...	1982	6216407
the epidemiological evaluation, in burma, of the skin test reagent lra6; a cell-free extract from armadillo-derived mycobacterium leprae. part 1: leprosy patients.		1982	6220986
the epidemiological evaluation, in burma, of the skin test reagent lra6; a cell-free extract from armadillo-derived mycobacterium leprae. part 2: close contacts and non-contacts of bacilliferous leprosy patients.		1982	6220987
clofazimine-resistant leprosy, a case report.		1982	6749703
histology of indeterminate leprosy.	skin biopsies from 20 patients with indeterminate leprosy were studied histopathologically. the most common clinical manifestation of the disease was a hypopigmented macule. in most cases, 5% to 10% of the dermis was occupied by the infiltrate. sweat glands were involved in two thirds of the cases, and approximately half the biopsies showed involvement of arrector pili muscle and pilosebaceous glands. ninety-five percent of the cases had involvement of the dermal nerves, with perineural infiltra ...	1982	6749705
tissue response to lepromin, an index of susceptibility of the armadillo to m. leprae infection--a preliminary report.	in this preliminary report the histopathological appearance of the lepromin reaction in armadillos was correlated with their response to infection with m. leprae. three different types of lepromin response were described, namely the lepromatous, the borderline, and the tuberculoid lepromin reactions. it was found that 10 out of the 11 animals with a lepromatous lepromin reaction and the one animal with a borderline lepromin reaction developed disseminated disease. the two with a tuberculoid lepr ...	1982	6749706
bcg vaccination in leprosy.		1982	6749707
co-incident (simultaneous) dapsone sensitive and dapsone resistant leprosy.		1982	6749709
serological activity of purified glycolipid from mycobacterium leprae.		1982	6749710
leprosy and its chemotherapy.		1982	6749788
improved leucocyte migration inhibition response of leucocytes from lepromatous leprosy patients with hapten modified m. leprae.	two acetoacetylated derivatives of mycobacterium leprae with variable hapten groups and a conjugate with tetanus toxoid were prepared. these were tested as antigens along with unmodified m. leprae in the leucocyte migration inhibition response of leucocytes from clinically, bacteriologically and histopathologically confirmed cases of lepromatous leprosy. lmi response was poor with m. leprae, but was significantly enhanced with acetoacetylated m. leprae.	1982	6751637
attempts at cultivation of mycobacterium leprae in cell culture under regulation of redox potential at environment.		1982	6752102
comparison of mycobacterial granulomas in guinea-pig lymph nodes.	a study was made of mycobacterial-induced granulomas in guinea-pig lymph nodes. live bcg (pasteur) induced a granuloma containing epithelioid cells while cobalt irradiated mycobacterium leprae induced a granuloma comprised of phagocytic macrophages. the granulomas were quantitated by measurement of lymph node weight and the areas of infiltration in histological sections. the time course of granuloma formation induced by co-irradiated m. leprae was veary different from the time course of the gran ...	1982	6752360
viability of m. leprae under normal and adverse atmospheric conditions.	harvested bacilli from fresh lepromas were subjected to drying at room temperature for varying periods, were exposed to direct sun-rays, uvr, and were then injected into the foot pads of thymectomised irradiated mice for checking the viability. the organisms could survive uvr for 30 minutes, direct sunlight for 2 hours and room temperature for 7 days. details are presented.	1982	6752575
biochemical correlation of m. vaccae with m. leprae.	the biochemical tests, namely, niacin, catalase, nitrate reduction, tween hydrolysis, tellurite reduction, arylsulfatase and urease tests were carried out for all the mycobacteria which are immunogenically closely related to m. leprae. among them only m. vaccae shows closest relationship with m. leprae when compared with its communicated data. except for the tellurite reduction test which was variable in case of m. leprae, all the other tests were found similar to that of m. leprae. in the next ...	1982	6752576
clinico-pathological study of so-called immune zones in leprosy.	the clinical, bacteriological and histopathological features were studied in 20 cases of leprosy (10 ll + 10 bl) from the so-called immune zones i.e. axilla, groin, and a narrow transverse band of skin over the lumbosacral region of the body. apparently uninvolved skin over the chest was studied as control site. in the so-called immune sites, the clinical lesions of leprosy were noted in 40% of the cases (7 ll + 1 bl), afb (both solid and granular forms) were detected in the smears of 45% cases ...	1982	6752577
discrepancy in the histopathological features of leprosy lesions in the skin and peripheral nerve. report of a preliminary study.	simultaneous biopsies of the skin and a cutaneous nerve were done in 36 randomly selected leprosy patients and the histopathological features of the lesion in the two tissues were compared. a discrepancy was noticed in 21 instances. in 19 of these cases the lesions in the nerve were found to be immunologically more deficient and were also bacillated, even when the skin lesion had cleared or regressed. the implications of the findings are discussed and the need for further studies is emphasized.	1982	6752578
leprosy: the epidemiology of a slow bacterium.		1982	6754406
sensitization or tolerance to mycobacterium leprae antigen by route of injection.	aqueous suspensions of heat-killed mycobacterium leprae in a dose of 10(7) organisms were highly immunogenic when injected intradermally (i.d.). the same dose of bacteria did not sensitize when given intraperitoneally (i.p.) or intravenously (i.v.), and did so only minimally at best when given subcutaneously. the i.d. route was the most immunogenic for sheep erythrocytes also. m. leprae injected i.p. or i.v. stimulated immune tolerance to m. leprae challenge i.d. in older mice (greater than or e ...	1982	6754621
class specific anti-mycobacterium leprae antibody assay in lepromatous leprosy (bl-ll) patients during the first two to four years of dds treatment.	previously, a slight decrease in antibodies against m. leprae antigen 7 was demonstrated after one year of dapsone (dds) treatment in 14 of 15 patients with lepromatous (bl-ll) leprosy. the same patients have now received dds from 11/2 to 4 years (median 3 years) and sera taken at the start, during, and at the end of the observation period have been retested for antibodies against m. leprae antigen 7 by radioimmunoassay and tested for iga-, igm-, and igg-anti-m. leprae antibody activity by solid ...	1982	6754639
significance of antibody studies in leprosy and experimental models of the disease.		1982	6754640
cultivation, the neglected priority.		1982	6754641
a comparison of the bactericidal activity of a series of rifampicins against mycobacterium leprae.	the bactericidal activity against mycobacterium leprae of six 3-(4-methylpiperazin-1-yl-iminomethyl)rifamycin sv (rifampicin) derivatives was compared with that of rifampicin in a modified proportional bactericidal test, using a smaller number of experimental animals and allowing the administration of a single dose of the drugs. no correlation was found between the in vitro activity of the rifamycin derivatives against m. tuberculosis or rapidly growing mycobacteria in vitro and the activity aga ...	1982	7037013
radiolabelled m. leprae resident in human macrophage cultures as an in vitro indicator of effective immunity in human leprosy.	twelve strains of human derived, freshly extracted m. leprae maintained within human macrophages showed a 2.1-13.2-fold increase in the incorporation of 3h-thymidine compared to parallel cultures containing heat-killed bacilli of the same strain. the addition of antigen stimulated lymphokines from five paucibacillary, tuberculoid leprosy patients resulted in the inhibition of the uptake of the radiolabel by 49-87%. minimal, or no, inhibition was noted in the presence of similar culture supernata ...	1982	6756718
iga, igm and igg anti-m. leprae antibodies in babies of leprosy mothers during the first 2 years of life.	iga, igm and igg anti-m. leprae antibody activity was estimated by solid phase radioimmunoassay in repeated serum samples from cord sera to sera taken 2 years after birth from 29 babies of mothers with lepromatous leprosy (group 1) and 16 babies of mothers with tuberculoid leprosy and non-leprosy control mothers (group 2). iga anti-m. leprae antibody activity could be detected in 30% and igm anti-m. leprae antibody activity in 50% of cord sera from group 1, but not in any of the cord sera from g ...	1982	6756719
drug resistance and multi-drug therapy in leprosy.		1982	6757576
solid, fragmented and granular index as one of the parameters in drug trials.	a bacteriological study of the skin smears of 108 bacteriologically positive cases of leprosy, 30 of them untreated, has been undertaken. in each case solid, fragmented and granular (sfg) index, bacteriological index (bi) and morphological index (mi) of skin smears from 4 sites were calculated. the results show that sfg index together with bi can be used as one of the parameters in drug trials. the importance of sfg index in research and as a routine laboratory procedure in leprosy control units ...	1982	6757578
effect of freezing mycobacterium leprae in tissues.	the effects of freezing tissues from m. leprae-infected armadillos as well as biopsy specimens from leprosy patients have been studied. no loss of viability of m. leprae was observed using mouse foot pad technique. similarly, using bioluminescent determinations of bacterial atp, it was shown that these bacilli retain all their metabolic activity during such freezing and thawing process. the practical implications of these findings have been discussed.	1982	6757579
[vaccination against leprosy].		1982	6758711
structure of the wall polysaccharide isolated from a group of corynebacteria.		1982	6759115
the use of subcellular components of bcg for studying host-mycobacterium interaction in relation to leprosy.		1982	6760793
use of a unit gravity sedimentation chamber for the purification of mycobacterium leprae.	the present paper summarizes results concerning a mild isolation method of mycobacterium lepraemurium and m. bovis-bcg from host tissues, and describes the application of such a method in purifying m. leprae (m1) from either infected armadillo tissues or human skin biopsies. this isolation method consists of homogenization, two-phase partition in dextranpolyethylene glycol and finally sedimentation in sucrose gradient using a unit gravity chamber. such a purified m1 preparation appears to be dev ...	1982	6762126
serological approaches for the characterization of catalase in tissue-derived mycobacteria.	cell-free extracts of mycobacterium lepraemurium from mouse liver and m. leprae from armadillo liver were analysed for the presence of any mycobacterial catalase by using the specific inhibitor 3-amino-1,2,4-triazole and seroprecipitation titrations. these studies clearly demonstrated the presence of a "t" type of mycobacterial catalase in m. lepraemurium and placed it, in terms of immunological distance, in a position between m. tuberculosis and m. avium. the results did not reveal any detectab ...	1982	6762127
chemotherapy of the mycobacterial diseases--leprosy and tuberculosis.		1982	6762341
attempts to establish host cells for mycobactrium leprae in vitro by hibridizing mouse macrophages and hela cells.		1982	6762775
adherence of mycobacterium leprae to macrophage as an indicator of pathogen induced membrane changes.		1982	6762986
immunotherapy with a mixture of mycobacterium leprae and bcg in different forms of leprosy and in mitsuda-negative contacts.	a total of 529 weak or non-reactors to m. leprae, including mitsuda-negative contacts and patients with leprosy, were vaccinated once or repeatedly, as necessary, with a mixture of 6 x 10(8) purified, heat-killed m. leprae and 0.01 mg to 0.2 mg of viable bcg. clinical, histopathological and immunological criteria were used to evaluate the response of these individuals. clinical changes, including sharper definition of borders and progressive flattening and regression of lesions, were observed in ...	1982	6763002
in vitro lymphocyte stimulation in patients with lepromatous and borderline tuberculoid leprosy. the effect of dapsone treatment on the response to mycobacterium leprae antigens, tuberculin purified protein derivative and non-mycobacterial stimulants.	lymphocytes from peripheral blood were isolated from leprosy patients and healthy contacts (hc) of leprosy patients and stimulated in vitro with: mycobacterium leprae and a m. leprae cell wall antigen, mlw 1; tuberculin purified protein derivative (ppd); antigens prepared from candida albicans, entamoeba histolytica, leishmania aethiopica, and parotitis virus; the non-specific mitogens phytohemagglutinin (pha) and concanavalin a (con-a). lymphocytes from patients with untreated lepromatous lepro ...	1982	6763003
the morphology of bacilli at different levels of the dermal infiltrate in lepromatous leprosy.	the morphological index (mi) of bacilli at three levels of the granuloma in 20 skin biopsies from untreated lepromatous patients has been recorded. the more superficial part of the granuloma and the subepidermal free zone showed a significantly higher mi, compared to the mid and lower zones. possible reasons for this non-uniform distribution of morphologically intact bacilli are discussed.	1982	6763004
failure to validate the growth of mycobacterium leprae on m-y 14b agar medium.	confirmation experiments on colony formation of m. leprae on the m-y 14b agar medium developed by murohashi and yoshida were carried out for two years independently by individual members of an organized research group, according to the method described by murohashi and yoshida. the results obtained can be summarized as follows: a) no colony production by m. leprae on m-y 14b agar medium was seen. b) no increase in the number of cells of m. leprae on m-y 14b agar medium during cultivation was see ...	1982	6763005
leprosy and tuberculosis.		1982	6763794
leprosy--a disease of the schwann cell.		1982	6763963
quantitative analysis of the sequence of nerve damage in experimental leprosy.		1982	6763964
a study of continuous bacillaemia in borderline and lepromatous type of leprosy.	the present work has established beyond doubt that bacillaemia occurs practically in all cases of borderline and lepromatous leprosy. the number of bacilli in the blood is significantly high, to the tune of 4,000 bacilli per ml. even so, it does not produce any adverse symptoms of septicaemia. special emphasis was laid in the present study for determining the continuity or otherwise of bacillaemia. some of the patients had continuous bacillaemia according to the criteria fixed in the present stu ...	1982	6763965
mouse foot-pad growth patterns of m. leprae in relation to morphological index.		1982	6763966
immune status of subsided cases of tuberculoid (tt) and borderline tuberculoid (bt) leprosy.	a comparative study of lepromin reactions (early and late), m. leprae induced lymphocyte blastogenesis, per cent t cell number in peripheral blood and immunoglobulin (igg, iga and igm) levels have been made in tt-active, tt-subsided, bt-active and bt-subsided leprosy cases. no significant difference has been noted amongst these groups in the above mentioned investigations except in subsided bt cases where 9 out of 11 cases failed to evoke any late skin reaction to dharmendra antigen. in addition ...	1982	6763967
susceptibility of the host--role of non-genetic factors.		1982	6763968
an appraisal of medical research in the treatment and control of leprosy.		1982	6763969
the microscopical pattern of growth of mycobacterium leprae-murium "douglas" in microcolonies.	a thin section technique of ogawa egg yolk culture medium inoculated with mycobacterium leprae-murium was found to reveal microscopical growth of the strain which could not be demonstrated by macroscopical examination. a peculiar structure of the growth, characterized by many lytic spots different in size, was observed indicating the possible presence of a temperate phage which may interfere with the synthesis of nucleic acids needed for the active multiplication of m. leprae-murium.	1982	6764333
dna isolated from mycobacterium leprae: genome size, base ratio, and homology with other related bacteria as determined by optical dna-dna reassociation.	dna derived from mycobacterium leprae (grown in armadillos) was isolated, purified, and analyzed spectrophotometrically. the genome size and guanine-plus-cytosine content of m. leprae were 1.3 x 10(9) and 55.8%, respectively. among selected strains of mycobacterial, nocardial and corynebacterial species, corynebacterium sp. 2628 lb, isolated from a human leprosy patient, showed the highest dna homology with m. leprae. of the dnas derived from mycobacteria, those of m. tuberculosis and m. scroful ...	1982	6764515
[comparison of the stamp specimens with histopathological tissue sections of the ear lobe].		1982	6764762
mast cell and mycobacterium leprae in experimental leprosy.	the association of mast cells and lepra bacilli was studied in the foot pad skin of immunosuppressed mice inoculated with 10(5) lepra bacilli. the mice were killed at intervals varying from 2 1/2-14 months postinoculation. m. leprae are found in large numbers in nerves, blood vessels, muscle fibres, hair follicles, sweat and sebaceous glands. mastocytosis and extensive degranulation are seen at similar sites. since these are the structures most affected in leprosy where the lepra bacilli and deg ...	1982	6764919
[acid-fast bacilli in the water of the lake of aleixo].	the presence of acid-fast bacilli in the waters of the lake of aleixo is recorded. this site is a hanseniasis colony. the physical-chemical environment of this lake is described by an annual cycle and it is intended to relate the quality of the water to the highest or to the lowest frequency of detected mycobacteria. it is suggested that the work in this lake system should be continued towards a more specific characterization of these microorganisms and the determination of their viability.	1982	6764920
chemotherapy of leprosy for control programmes.		1982	6806990
bacteriology of mycobacterium leprae: state of the art paper.		1982	6810740
pyrolysis mass spectrometry: its applicability to mycobacteriology, including mycobacterium leprae.		1982	6810741
ultrastructure of mycobacterium leprae and other acid-fast bacteria as influenced by fixation conditions.	a procedure using aldehydes, oso4, ca++ and uranyl acetate was selected for study of the fixation of mycobacterium leprae in skin biopsies of leprosy patients. the ultrastructural pattern of recognized normal m. leprae cells fixed by the above procedure was characterized, and was found to be similar to that of other acid-fast bacteria fixed by the same procedure, except for the geometry of the membrane profile. under such fixation conditions, this profile is always asymmetric in in vitro-culture ...	1982	6810742
hla-dr-associated genetic control of the type of leprosy in a population from surinam.	the relationship between hla phenotype and leprosy classification was studied in 73 unrelated patients and 92 healthy controls from a mixed negroid-caucasoid population originating from surinam, south america. heterogeneity in the distribution of hla-dr (but not a, b, and c) was detected between tuberculoid (tt* + bt) leprosy and lepromatous (bl + ll*) leprosy patients (p = 0.024). this heterogeneity appeared to be caused almost exclusively by dr3. most significantly, the frequency of dr3 was ...	1982	6811517
[immunotherapy of leprosy in 1981].		1982	6815984
[2 cases of paucibacillary leprosy resistant to dapsone].	two patients are presented with a primary resistant bt leprosy. diagnosis was made on a clinical base: luck of improvement under supervised sulfone treatment and quick regression of the lesions under a short course of rifampicine (8 weekly doses). the two children lived together with their lepromatous mothers who are suspected of dds resistance. where secondary dds resistance is found only in multibacillary leprosy, primary resistance shows up in all clinical forms of leprosy.	1982	6815986
[prevalence of dapsone-resistant leprosy in burundi. temporary results].		1982	6815995
[current data on the bacteriology of leprosy].	microscopic examination and mouse foot-pad inoculation are the main tools in mycobacterium leprae bacteriology. microscopic examination enables to calculate bacteriological (ib) and morphological (im) indexes. the former scores the density of acid-fast organisms in the lesions and the latter scores their viability since only solidly stained acid-fast organisms are likely to be alive. mouse foot-pad inoculation provides the opportunity for studying the growth curve of m. leprae. alterations of th ...	1982	6815996
[mycobacterial strains adapted to culture from human lepromas. identifying characteristics relative to mycobacterium leprae].		1982	6815997
[morphologic and biologic properties of diphtherroid organisms (ldc) isolated in leprosy].	acid-alcohol-fast bacteria are not always detectable in all leprosy lesions. non acid-fast microorganisms may be associated with acid-fast bacteria. the most frequently isolated strains from leprosy lesions are non acid-fast bacteria, morphologically related to c. diptheirae. hence their designation as diphtheroïds or ldc (leprosy derived corynebacteria). their antigenic structure is more closely related to m. leprae and other mycobacteria than to classical corynebacteria. this leads to the hypo ...	1982	6815998
[comparative bactericidal action of rifampicin alone on m. tuberculosis and m. leprae].		1982	6815999
[resistance of mycobacterium leprae to sulfones (dds) and rifampicin in recurring lepromatous leprosy in martinique and guadeloupe since february 1980].		1982	6816000
[the lymphocyte transformation test using phytohemagglutinin, lepromin and m. leprae in lepromin-negative and positive leprosy: prognostic value compared with that of lepromin].		1982	6816002
[employment of a program in the leprosy campaign in mayotte based on the use of rifampicin and clofazimine].		1982	6818815
replication of mycobacterium leprae in hibernating ground squirrels (citellus tridecemlineatus).	subcutaneous injections of 10(6) acid-fast bacteria from human lepromatous tissue to hibernating ground squirrels (citellus tridecemlineatus) is followed by generalized infection of the animals and spontaneous death. the number of mycobacteria is high in the skin of gs during winter and decreases during summer. animals surviving the first hibernation period invariably die during the second. the number of microorganism in the visceral organs is low at all times. the system of infected hibernating ...	1982	6818816
biochemical studies with leprosy-derived corynebacteria.		1982	6818817
biological properties of diphtheroïd bacteria (ldc) isolated from human leprosy lesions.		1982	6818818
[diphtherimorph germs linked to leprous bacillas].		1982	6819754
ribosomal antigens of mycobacterium leprae.		1982	6819797
is diphenoloxidase a constitutive enzyme in mycobacterium leprae?		1982	6821119
isolation of fastidiously growing mycobacteria from armadillo livers infected with mycobacterium leprae.		1982	6890545