Publications

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[detection of healthy carriers of clostridium perfringens with thermoresistant spores in the personnel working in the kitchens of two roman hospitals]. 19704329407
[septic gas gangrene with unusual focus of infection]. 19704333357
effect of intramuscular or intrahepatic injections of clostridium perfringens on rabbit serum lactate dehydrogenase.serum lactate dehydrogenase (ldh) activity, ldh isoenzyme pattern, phospholipase c activity, phosphorous level, hemoglobin, and erythrocyte osmotic fragility were followed in rabbits after intramuscular (im) or intrahepatic (ih) injections of clostridium perfringens. on the first day after im injection, there was a drop in ldh activity; this was followed by an increase of ldh activity on the third and sixth day. on the seventh day, ldh activity began to decline, and by the ninth day it had almos ...197016557808
mechanism of action of the enteropathogenic factor of clostridium perfringens type a.cell extract of an enteropathogenic strain of clostridium perfringens type a was administered intravenously to lambs, rabbits, and guinea pigs. lambs developed transitory diarrhea, lacrimation, salivation, nasal discharge, lassitude, and dyspnea in 1 to 5 hr after inoculation. large doses of the inoculum caused rapid onset of the clinical signs and subsequent death. examination of dead animals revealed intensely hyperemic small intestinal mucosa and some congestion in the liver, lungs, spleen, a ...197116557924
clostridium perfringens type a food poisoning i. response of the rabbit ileum as an indication of enteropathogenicity of strains of clostridium perfringens in monkeys.diarrhea and vomiting have been experimentally produced in monkeys after oral challenge with viable cells or culture filtrates of certain strains of clostridium perfringens that previously had been shown to produce either fluid accumulation in the ligated ileum or overt diarrhea in the nonligated ileum of the rabbit, or both. strains (or their culture filtrates) which did not produce a response in the rabbit likewise produced no symptoms in monkeys.197116557936
clostridium perfringens type a food poisoning ii. response of the rabbit ileum as an indication of enteropathogenicity of strains of clostridium perfringens in human beings.the effect of feeding human beings individual strains of clostridium perfringens or culture filtrates thereof was examined. the strains selected for challenge included both those which had previously been shown to produce fluid accumulation in the ligated ileum or overt diarrhea when injected into the nonligated ileum of the rabbit, or had produced both, and those which did not regularly produce these responses. challenge doses prepared by allowing each strain to grow in beef stew for 3 hr at 46 ...197116557937
intestinal strangulation in clostridium perfringens-monocontaminated rats.ischemic and hemorrhagic strangulation of closed intestinal segments was studied in germ-free rats individually contaminated with one of seven separate strains of clostridium perfringens type a. the principal findings were that (i) the monocontaminated rats with ischemic or hemorrhagic intestinal strangulation died at the same rapid rate; (ii) intraintestinal blood did not augment the action of c. perfringens in the presence of ischemic strangulation; (iii) although some strains were more toxic ...197116557999
new quantitative, qualitative, and confirmatory media for rapid analysis of food for clostridium perfringens.[this corrects the article on p. 501 in vol. 21.].197116349911
[combined effect of morphocycline and erythromycin on the pathogenic clostridia and experimental anaerobic (gaseous) infection]. 19714333644
[distribution of clostridium perfringens in pasture soil]. 19714333884
[kinetic properties of clostridium welchii sr-12 glutaminase and effect of some ions on its activity]. 19714333906
studies on n-acetylneuraminic acid aldolase.n-acetylneuraminic acid aldolase from clostridium perfringens was irreversibly inactivated by 1mm-bromopyruvate with a half-life of 4.2min at ph7.2 and 37 degrees c. the rate of inactivation was diminished in the presence of pyruvate but not with n-acetyl-d-mannosamine, indicating that the inhibitor acted at, or close to, the pyruvate-binding site. the apparent k(i) for bromopyruvate, calculated from the variation of half-life with inhibitor concentration, was 0.46mm, compared with a competitive ...19714333937
bacteriological studies of fatal clostridium perfringens type-a infection in chickens. 19714334070
human erythrocyte membrane glycoprotein: a re-evaluation of the molecular weight as determined by sds polyacrylamide gel electrophoresis. 19714334138
isolation of glycopeptides from low- and high-density platelet plasma membranes. 19714334583
[the present state concerning food-borne diseases]. 19714334606
inhibition of acylneuraminate pyruvate-lyase: evidence of intermediary schiff's base formation and of a possible role of histidine residues. 19714334714
buoyant density gradient centrifugation of rna and dna in alkali iodide solutions. 19714334851
immunochemical properties of purified polysaccharide fraction of clostridium perfringens type a. 19714334860
[gas gangrene following intramuscular injection]. 19714334927
[cl. welchii as a potential cause of food poisoning]. 19714335191
[durability of clostridium perfringens toxins in tissue of dead animals]. 19714329469
pathogenesis of bacterial diarrheas (second of two parts). 19714329624
[some characteristics of proteolytic systems of cl. perfringens type a]. 19714332196
mechanism of the phosphorylation of thymidine by the culture filtrate of clostridium perfringens and rat liver extract. 19714332208
[aggregating action of phospholipase c of clostridium welchii on human platelets]. 19714332362
[myocardial ultrastructure of a rabbit in the course of poisoning with the toxin of clostridium perfringens type a]. 19714332400
resistance of vegetative cells of clostridium welchii to low ph. 19714332569
identification of n,o-diacetyl-,n-acetyl-, and n-glycolylneuraminyl-(2-3)-lactose in rat urine. 19714332836
purification and characteristics of the enterotoxin of clostridium perfringens type a. 19714333491
rapid detection of clostridium perfringens enterotoxin by a modified ligated intestinal loop technique in rabbits. 19714333492
[intracytoplasmic membrane structures in clostridium perfringens]. 19714333506
[31 cases with anaerobic bacteria as the only isolates]. 19714333548
arthritis in pigs induced by dietary factors. microbiologic, clinical and histologic studies. 19714335983
[biological features of strains of cl. perfringens isolated during toxinfections]. 19714336037
collagenolytic enzymes. 19714336709
[a clostridium perfringens mutant producing coatless spores by lysozyme-dependent germination]. 19714337134
phospholipase c from clostridium perfringens: purification by electrophoresis on acrylamide-agarose gel. 19714337394
reversible resolution of glutamate decarboxylase from clostridium perfringens. 19714337395
[excretion of clostridium perfringens by black flies]. 19714337682
[comparative microbiological studies of contaminated soils. ii. qualitative changes of microbial species]. 19714337692
[anaerobic sepsis following abortion]. 19714338536
[a new class of welchicins]. 19714339610
biological characteristics of clostridium perfringens type a enterotoxin.an enterotoxin with the ability to induce fluid accumulation in rabbit ileal loops, erythema in the skin of guinea pigs, and lethality in mice appears in cell extracts (ce) and culture filtrates (cf) of sporulating cells of some clostridium perfringens type a strains. all activities in ce and cf were eluted simultaneously from a sephadex g-200 column. different elution patterns were obtained for these activities present in ce and cf. rabbit immune serum against cf and the active ce fractions eli ...19714343403
[treatment of gas gangrene]. 19714339150
some investigations concerning the growth characteristics of entamoeba histolytica in monoxenic association in a new culture medium. 19714346868
[biological properties of clostridium, isolated from food products during preservation]. 19714348513
[how to obtain specific pathogen-free mice by the antibiotic decontamination method]. 19714333291
[use of tissue extracts in the treatment of cancer]. 19714349142
incidence of clostridium perfringens in dehydrated soup, gravy, and spaghetti mixes. 19714353455
haemagglutination reaction with avian myeloblastosis virus. 19714397346
an assay for intercellular adhesive specificity.a modification of an assay for intercellular adhesive specificity is described. the method involves the collection of radioactively labeled cells by aggregates of the same (isotypic aggregates) or different (heterotypic aggregates) types of tissue and determination of the number of cells collected by liquid scintillation counting. the use of (32)p to label the tissues permitted a much more rapid estimation of cell collection than was obtained previously. with the use of chick embryo neural retin ...19714398789
[filtration or centrifugation for production of alpha-toxin from fluid clostridium-welchii cultures]. 19714400727
structural analysis of phosphatidylcholine of plant tissue.pure preparations of phosphatidylcholine were isolated from spinach leaf chloroplasts, spinach leaf microsomes, and cauliflower inflorescence. the isolated phosphatidylcholine was treated with snake venom phospholipase a, and the fatty acid distribution and composition of the fatty acid methyl esters prepared from the lysophosphatidylcholine and the freed fatty acid were determined by gas-liquid chromatography. the results showed that saturated fatty acids were preferentially esterified at posit ...19714362139
changes in the microflora of man during long-term confinement.extended space missions may change the human normal microflora, including the intestinal flora. the bacterial composition of the intestinal microflora is an important factor in natural resistance to infection. during the year-long medico-engineering experiment human intestinal microflora was thoroughly studied. it was necessary to collect more detailed information concerning the composition in healthy people. 83 microbiological tests of faeces of 27 healthy male subjects were carried out. it was ...197111942343
emphysematous cholecystitis: a report of five cases. 19714321478
endemic goiter in greece: some new epidemiologic studies. 19714321500
lipid layer in cell membrane. 19714321338
pyrimidine-specific antibodies: reaction with dna's of differing base compositions. 19714322722
tolerance of bacteria to high concentrations of nacl and glycerol in the growth medium.when compared at similar levels of water activity, glycerol was more inhibitory than sodium chloride to relatively salt-tolerant bacteria and less inhibitory than salt to salt-sensitive species.19714322678
lecithin-agar assay for lecithinase antibodies in serum.a technique for assay of lecithinase antibodies in serum was developed in this laboratory by using a lecithin-agar plate diffusion procedure based on a combination of described plate assays. egg yolk lipoprotein composed primarily of lecithin was used as a substrate for reaction with free or non-neutralized lecithinase c after incubation of known amounts of lecithinase c with various dilutions of control and test sera. it was found that the size of the reaction zone was a function of enzyme conc ...19714322282
n-acetylneuraminic acid analogues. ii. the action of n-acetylneuraminic acid aldolase on 8-carbon and 7-carbon analogues. 19714322517
immunological and biological activity of hcg following progressive desialylation. 19714322050
[retroperitoneal clostridium-perfringens-abscess]. 19714325550
purification and some properties of kappa toxin of clostridium perfringens. 19714325624
[purification and some properties of cl. perfringens lecithinase]. 19714325641
an epidemic of food-associated pharyngitis and diarrhea. 19714325668
acute bloat syndrome in stumptailed macaques (macaca arctoides): a report of four cases. 19714325689
intestinal haemorrhage syndrome in artificially-reared lambs. 19714325723
[identification of cl. perfringens types b, c, and f in tissue culture]. 19714325871
studies in the alkaline denaturation of dna and the stability of the helix. 19714325156
[etiology and epizootiology of swine dysentery]. 19714325232
[clostridium intoxications in swine]. 19714325233
effect of clostridium perfringes beta antitoxin and colostral absorption on incidence of calf scours. 19714325252
clostridium welchii septicotoxemia. a review and report of 3 cases. 19714324790
molar ratio of simple sugars in bacterial fractions of cl. perfringens type a. 19714324795
identification of isolates of clostridium perfringens types c and d by agglutination and fluorescent-antibody methods.agglutination and fluorescent-antibody methods were employed for screening clostridium perfringens types c and d from 393 isolates of this organism. all of 50 strains which were isolated in japan and were agglutinable with an antiserum prepared against a stock strain of type c no. 3182 toxigenically belonged to type c, but the antiserum showed no cross-agglutination with any of type c strains isolated in denmark. all of the latter strains, however, were agglutinated by an antiserum prepared agai ...19714324883
medium for toxin production by clostridium perfringens in continuous culture.a tryptone-salts medium for continuous growth and alpha toxin production of clostridium perfringens type a in an adapted chemostat is described. in such steady-state cultures, fermentative and biochemical activity of c. perfringens remained unchanged. toxigenic ability to produce alpha, theta, and nu toxins was preserved.19714324884
comparison of media for the enumeration of clostridium perfringens.for the enumeration of viable vegetative cells and spores of clostridium perfringens, noncommercial (laboratory prepared) sulfite-polymyxin-sulfadiazine (sps) agar, tryptone-sulfite-neomycin (tsn) agar, and shahidi-ferguson-perfringens (sfp) agar were statistically compared to sps agar without antibiotics. the selectivities of these four media were also evaluated on the basis of their ability to inhibit the growth of pure cultures of a variety of other organisms. the average recovery of vegetati ...19714324885
bacteriocins of clostridium perfringens. 1. isolation and preliminary studies. 19714324544
studies on a neuraminidase containing gonadotropin inhibitor substance in bovine pituitary extracts. 19714324681
[gas gangrene after common bile duct exploration]. 19714324705
analysis of clostridium botulinum toxigenic types a, b, and e for fatty and carbohydrate content.lyophilized, 48-hr log-phase vegetative cells were extracted with chloroform-methanol (2:1, v/v) and ethanol-ether (3:1, v/v) and then saponified with methanolic koh. gas-liquid chromatography of the methyl esters of extractable fatty acids revealed distinctive "pattern profiles" of clostridium botulinum toxigenic types "a," "b," and "e." c. perfringens type "a" and escherichia coli strain "b" were also studied in a similar manner and were found to give pattern profiles which were distinct even ...19714324194
new quantitative, qualitative, and confirmatory media for rapid analysis of food for clostridium perfringens.a selective and differential medium, shahidi-ferguson perfringens agar (sfp agar), and a confirmatory medium, lactose-motility agar (lm agar), were developed for the enumeration and identification of clostridium perfringens in foods. these media provide a rapid, specific, and direct diagnosis of c. perfringens. sfp agar contains sodium metabisulfite and ferric ammonium citrate to demonstrate h(2)s production and egg yolk to demonstrate lecithinase production by c. perfringens. on sfp agar, c. pe ...19714324195
the susceptibility of clostridium perfringens type a to cephalosporin c antibiotics in vitro. 19714324251
gas gangrene infections related to compound fractures. 19714324351
dna polymerase activity in homogenates of cells infected with mc29 virus. 19714323849
immunization of sheep against experimental clostridium perfringens type a enteritis. 19714323916
structural changes in cells of clostridium perfringens infected with a short-tailed bacteriophage. 19714323917
[morphological and some histochemical changes in the organism of guinea pigs caused by hyaluronidase from cl. perfringens type a]. 19714329698
effect of phospholipase and trypsin on histidine uptake by mouse brain slices. 19714329756
the binding of ouabain to na + -k + -dependent atpase treated with phospholipase. 19714329761
conformation changes of deoxyribonucleic acid and polydeoxynucleotides in water and ethylene glycol. 19714329872
[bacteriologic control in decubitus ulcer]. 19714329903
purification of neuraminidases from vibrio cholerae, clostridium perfringens and influenza virus by affinity chromatography. 19714330046
insulin-like effect of clostridial phospholipase c, neuraminidase, and other bacterial factors on brown fat cells. 19714330059
[pneumocholecystitis]. 19714330098
thymidine kinase of rat liver. activation by phospholipase c and subcellular distribution in the liver.1. the thymidine kinase activity of rat liver is greatly enhanced on addition of phospholipase c to the assay mixture. 2. most of the thymidine kinase activity of the liver is recovered in the mitochondrial and in the impure ;nuclear' fractions. no activity was detected in purified nuclei prepared in high-density sucrose. 3. a substantial thymidine kinase activity could be detected, with the aid of phospholipase c, in all rat tissues examined.19714330199
a method for the preparation of radioactive fsh with preservation of biologic activity. 19714330528
[sensitizing properties of purified concentrated cl. perfringens toxoids]. 19714330843
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