Publications

skin microflora and bacterial infections of the skin.	the skin is a milieu for controlled bacterial growth. skin supports the growth of commensal bacteria, which protect the host from pathogenic bacteria. environmental and local factors, host immunity, and organism adherence and virulence are intricately related to cutaneous infection. resident gram-positive bacteria include staphylococcus, micrococcus, and corynebacterium sp. staphylococcus aureus and streptococcus pyogenes are notoriously pathogenic in the skin. in order for bacteria to be pathog ...	2001	11924823
consistency of autoradiographic dna repair in cells treated "in vitro" with n-diazoacetylglycine amide. a preliminary approach to quantitate the absolute amount of dna repair.		2001	98772
[medical management of tachycardias (author's transl)].	guidelines for a step-wise plan of treatment of tachycardias have been compiled based on clinical empirical experience and with the aid of surface electrocardiograms, intracardial electrograms and stimulation techniques. the plan is primarily with the aid of surface electrocardiograms, intracardial electrograms and stimulation techniques. the plan is primarily oriented with respect to the antiarrhythmic efficacy, the adverse reactions and the practicability of the respective agents. any type of ...	2001	93075
visual aids in nutrition education. a guide to their preparation and use.		2001	95011
[volvulus of the afferent loop after billroth ii operation (author's transl)].	a case report is given of a patient, who had a volvulus of the afferent loop after billroth ii operation; on relaparotomy it was found, that this loop had slipped cranially through the mesocolic slit, twisted, and become gangreneous, due to bad adjustement of the slit. increased levels of serum amylase initially had given rise to the suspicion, that the deteriorating condition of the patient was due to pancreatitis. the patient recovered fully after resection had been done and an y-roux-anastomo ...	2001	94934
perinatal mortality.		2001	93220
[diagnostic and therapy of postoperative cardiac low-output-syndrome by means of intraaortic balloon pumping (iabp) or external counterpulsation (ecp)--a comparison (author's transl)].	a beneficial influence on the myocardial energy imbalance of the hypoxaemically damaged heart can be reached with either intraaortic balloon pumping (iabp) or external counterpulsation (ecp). in the realm of our acquired clinical experience with two groups of patients, the technical demands of these cardiac assist devices are discussed and distinguished from one another in relation to different indications.	2001	1084037
ventricular arrhythmias: some pathophysiological aspects and therapeutic problems.		2001	95451
origin of maternal serum alpha-fetoprotein.		2001	93217
an improved immunoelectrophoretic methodology for the evaluation of protein caloric malnutrition.	an improved technique of two dimensional immunoelectrophoresis of serum proteins on gelatinized cellulose acetate for routine evaluation of protein nutritional state is described. the method, which allows the estimation of more than 30 protein fractions, proves extremely useful in monitoring the effectiveness of total parenteral nutrition and protein metabolic conditions. sera samples from healthy subjects and cachectical neoplastic patients have been examined and preliminary results are reporte ...	2001	92995
[evolution of the free thyroxine index during pregnancy].		2001	95054
severe handicap in the newborn: treatment, non-treatment, or assisted death.		2001	92640
[evaluation of the beta-galactosidase test for the identification of enterobacteria].		2001	92602
[surgery of hepatic hydatic cyst. per-operative complications. possibility of anaphylactic etiology (author's transl)].	among fifty cases hepatic hydatid cysts, we have observed four serious complications during operation. these complications appeared at the time of the puncture of the cysts with a severe shock in three cases. anaphylactic etiology can be suspected but not proved without specific tests. because the severity of these accidents (one death), anesthesiologist must be aware of the possibility of such complications: it is important to recognize anaphylactic reactions during surgical procedure to instit ...	2001	95469
[antigen-specific t-cell factors in the regulation of the immune response (author's transl)].	we have examined in this brief review several facets of antigen-specific t-cell mediators found independently by different investigators. these factors have many common properties: (1) they are produced as a consequence of antigen stimulation; (2) they are obtained from t-cell populations and not from b cells or macrophages; their synthesis by t cells, however, has not been formally demonstrated; (3) they are antigen-specific and are selectively adsorbed by antigen-sepharose columns; (4) they la ...	2001	95085
paediatric surgeons are necessary.		2001	93224
ataxia-telangiectasia and hepatocellular carcinoma.	ataxia-telangiectasia (at) is a multi-system disease involving the cerebellum, cutaneous blood vessels and the immune system including both cellular and humoral components. it also involves hematological, endocrine and peripheral nervous systems. this disease is often associated with abnormal liver function tests, such as, raised alkaline phosphatase and various nonspecific histological changes in the liver. high incidence of various malignancies involving lymphoreticular, gastrointestinal and m ...	2001	92892
anticonvulsant action of a 1,5-benzodiazepine, clobazam, in reflex epilepsy.	clobazam, an anxiolytic 1,5-benzodiazepine, has been evaluated as an anticonvulsant in 2 animal models. in mice showing sound induced seizures, clobazam, 1--4 mg/kg, i.p., blocked seizure responses for 1--2 hr. in senegalese baboons papio papio showing photically induced myoclonus or seizures, clobazam, 2--12 mg/kg, i.v., totally prevented such responses for up to 6 hr. in baboons pretreated with allylglycine, 170--185 mg/kg, a similar but briefer protection was induced by clobazam. neurological ...	2001	98317
[long-term results of synchronized radiotherapy in inoperable orofacial squamous cell carcinoma].		2001	95490
reconstructive operations on tendons and a tendo-ligamentous apparatus.		2001	92871
water-treated stimulatory cells in the unidirectional mixed lymphocyte culture.		2002	129896
[an invariant of plasma viscosity].	determination of "m" invariant cannot lead to an absolute measurement of plasmatic viscosity, because "m" increases with temperature. it allows however to compare data determined in various conditions.	2002	126722
[details of a denounced physician (e.n.t.) suspected of bodily injury, and the following expert judgement (author's transl)].	an otologist was denounced and the patient's case history was confiscated because after tonsillectomy an absecess and a thrombosis of the vena jugularis interna had developed. the procedure and the medical aspect how at first being judged by the lawyer and the public prosecutor are described. on the base of the given medical opinion the court quashed proceedings. because of the growing number of denouncements this casuistic paper ends with four guiding principles to protect against unjustified m ...	2002	135139
[treatment of serous otitis].		2002	134426
[2 cases of sudden death of twins with idiopathic cardiomegaly].		2002	129892
[work of the department of children's ophthalmological services concerning the treatment of strabismus and amblyopia].		2002	128723
differences in length of hospital stay for medicaid and blue cross patients and the effect of intensity of services.		2002	113844
long-term treatment with beta-blockers after myocardial infarction.	162 patients discharged from hospital after mycardial infarction were randomly allocated to two groups, one received alprenolol 400 mg daily and the other served as the control. the period of follow-up was two years and all other treatment given was standardized. the two groups did not differ with respect to risk factors for myocardial infarction, the course of the acute infarct or treatment during follow-up. after two years one patient in the group treated with alprenolol had died suddenly as c ...	2002	134897
[the role of the auxilliary health personnel in the prevention of iatrogenic quadriceps contracture].		2002	120064
editorial: health care crisis.		2002	124076
[acid glycosaminoglycans in localized pleural mesothelioma--histochemical and biochemical study].		2002	135110
a fish hook and liver disease: revisiting an old enemy.	vibrio vulnificus is an uncommon but potentially devastating pathogen. early recognition with prompt antimicrobial therapy and surgical treatment are key factors for a favorable outcome. patients with diseases of the liver represent the group at highest risk of infection. however, clinicians are often unaware of underlying liver disease in these patients at the time of presentation. we present a case of fulminant v. vulnificus infection in a patient with previously undiagnosed liver disease.	2002	11892879
upper extremity infections following common carp fish (cyprinus carpio) handling.	upper extremity infection caused by aquatic pathogens on fish is a well recognized clinical entity. we report five consecutive cases of upper extremity infections, ranging from a simple localized reaction to a life-threatening systemic illness, which developed after handling common carp fish (cyprinus carpio). in four cases, infection occurred following a penetrating injury by either the bones or the fin spines of the fish. vibrio vulnificus was isolated from wound aspirates in four cases. early ...	2002	11895351
[recurrence in soft tissues of a giant cell tumor of the femoral condyle].	a case of recurrence with osteogenesis only in the soft tissues of a giant cell tumour is reported.	2002	128069
clinical comparison between glibornuride (glutril) and tolbutamide in maturity-onset diabetes: a controlled double-blind trial.		2002	134878
specificity of a heme-assimilating system of vibrio vulnificus to synthetic heme compounds.	vibrio vulnificus strain l-180, a clinical isolate, can obtain iron from a synthetic heme, iron-tetra(4-sulfonatophenyl)porphyrin (fe-tpps), as well as from a natural heme, protoheme. this assimilation of iron bound to tpps was demonstrated to be a common property of v. vulnificus by testing a total of 27 strains isolated from both clinical and environmental sources. strain l-180 could also utilize fe-tcpp, but not fe-tmpyp, as a sole iron source. tpps or its complex with a metal ion reduced bac ...	2002	11934497
vibrio vulnificus septicaemia.	vibrio vulnificus is an opportunistic pathogen capable of causing a fulminant septicaemia in susceptible patients. underlying chronic diseases such as liver impairment and immunosuppression are important factors contributing to the severity of the infection and outcome. early suspicion and diagnosis with appropriate antibiotic therapy is important as delay can adversely affect outcome. for those who develop tissue necrotizing fasciitis, early surgical debridement is recommended to allow better p ...	2002	11939447
vibrio vulnificus and the high risk. patient: a potentially fatal disease.		2002	11942106
identification of the cadba operon from vibrio vulnificus and its influence on survival to acid stress.	by a transposon-tagging method, cadba genes encoding a lysine/cadaverin antiporter and a lysine decarboxylase were identified and cloned from vibrio vulnificus. the deduced amino acid sequences of cadba were 64-97% similar to those reported from other enterobacteriaceae. functions of cadba genes on acid tolerance were assessed by comparing acid tolerances of v. vulnificus and its isogenic mutants, whose cadba genes were separately inactivated by allelic exchanges. the results demonstrated that g ...	2002	11959444
vibrio vulnificus necrotising fasciitis and septicaemia.		2002	11999222
vibrio vulnificus septicemia and leg ulcer.	vibrio vulnificus is a gram-negative bacteria that can cause septicemia, wound infection, or a self-limiting diarrhea. this infection typically presents as an extremely virulent infection in patients with underlying liver disease 1 to 2 days after exposure. we report a case of v vulnificus septicemia, cellulitis, and leg ulceration in a patient who had symptoms develop after exposure to brackish water (19 days before admission) or after ingestion of raw oysters (10 days before admission). the lo ...	2002	12004295
cytotoxic mechanism of vibrio vulnificus cytolysin in cpae cells.	vibrio vulnificus is an estuarian bacterium that causes septicemia and serious wound infection. the cytolysin, one of the important virulence determinants in v. vulnificus infection, has been reported to have lethal activity primarily by increasing pulmonary vascular permeability. in the present study, we investigated the cytotoxic mechanism of v. vulnificus cytolysin in cultured pulmonary artery endothelial (cpae) cells, which are possible target cells of cytolysin in vivo. v. vulnificus cytoly ...	2002	12005177
clearing mechanisms of vibrio vulnificus biotype i in the black tiger shrimp penaeus monodon.	vibrio species' infections are a common sequelae to environmental stress or other disease processes in shrimp, but the mechanism by which the shrimp eliminate the bacteria is poorly understood. in this study, the penetration, fate and the clearing of v. vulnificus were investigated in penaeus monodon. a bacterial disease isolate from a shrimp farm was identified as v. vulnificus biotype i. polyclonal antiserum was raised in rabbits against the bacterium and the specificity was verified by elisa ...	2002	12005240
an investigation into the changed physiological state of vibrio bacteria as a survival mechanism in response to cold temperatures and studies on their sensitivity to heating and freezing.	to induce pathogenic vibrio bacteria into a changed physiological state, in response to cold temperatures in sea water, and assess their sensitivity to heating and freezing, as compared with normal cells.	2002	12010547
occurrence of pathogenic vibrios in coastal areas of france.	this study was carried out to investigate the occurrence of potentially pathogenic species of vibrio in french marine and estuarine environments.	2002	12010553
proinflammatory cytokine profile in vibrio vulnificus septicemic patients' sera.	vibrio vulnificus causes a fulminant and frequently fatal septicemia in susceptible hosts. the present study was designed to evaluate the proinflammatory cytokine profile in v. vulnificus septicemia patients' sera and the effect of doxycycline therapy on the levels of proinflammatory cytokines. levels of proinflammatory cytokines, tumor necrosis factor (tnf)-alpha, interleukin (il)-1beta and il-6, were measured in the sera of v. vulnificus septicemic patients and normal healthy volunteers using ...	2002	12052568
[a successful treatment of vibrio vulnificus infection].	a 65-year-old male patient with a history of alcoholism visited our outpatient clinic complaining of nausea and diarrhea followed by dizziness. erythema and swelling with partial exfoliation on the right forearm to hand and right thigh were noticed. vibrio vulnificus was isolated from the purulent discharge of the skin. due to urgent and intensive treatment of bacterial shock and antimicrobial drugs, the patient fully recovered three months later. we believe that the patient survived from this f ...	2002	12073576
induction of an outer membrane protein of 78 kda in vibrio vulnificus cultured in the presence of desferrioxamine b under iron-limiting conditions.	although vibrio vulnificus is known to be able to utilize ferrioxamine b as an iron source, its outer membrane receptor remains to be determined. in this study, we found that v. vulnificus expressed a new outer membrane protein of 78 kda when grown in the presence of desferrioxamine b under iron-limiting conditions. the desferrioxamine b-dependent iron uptake was only observed in bacterial cells expressing this protein. furthermore, non-denaturing polyacrylamide gel electrophoresis followed by a ...	2002	12076789
a rapid and improved method for the detection of vibrio parahaemolyticus and vibrio vulnificus strains grown on hydrophobic grid membrane filters.	dna probe-based detection methods were developed and characterized as an alternative to time-consuming and less specific conventional protocols. digoxigenin-labeled probes were prepared by polymerase chain reaction amplification of the targeted sequences in the specific amplicons generated from genomic dna. specific probes with high yields were generated for the detection of the tlh gene of vibrio parahaemolyticus and the cth gene of v. vulnificus. colony (southern) hybridization analyses were c ...	2002	12092721
resistance of cold- and starvation-stressed vibrio vulnificus to heat and freeze-thaw exposure.	the effects of cold storage and starvation on the subsequent heat resistance and freeze-thaw resistance of vibrio vulnificus were studied. three strains of v. vulnificus were evaluated. cold stress had no effect on freeze-thaw resistance (p > 0.05). starvation enhanced freeze-thaw resistance for one strain compared to controls (p < 0.05). v. vulnificus was not heat resistant; control populations were inactivated within 12 min at 47 degrees c. starvation increased heat tolerance for one strain, b ...	2002	12092731
litr, a new transcriptional activator in vibrio fischeri, regulates luminescence and symbiotic light organ colonization.	vibrio fischeri is the bacterial symbiont within the light-emitting organ of the sepiolid squid euprymna scolopes. upon colonizing juvenile squids, bacterial symbionts grow on host-supplied nutrients, while providing a bioluminescence that the host uses during its nocturnal activities. mutant bacterial strains that are unable to emit light have been shown to be defective in normal colonization. a 606 bp open reading frame was cloned from v. fischeri that encoded a protein, which we named litr, t ...	2002	12100554
vibrio vulnificus infection in patients with liver disease: report of five autopsy cases.	five autopsy cases of vibrio vulnificus infection with liver disease are reported. all five patients ate raw seafood 24 h before the onset of illness. the clinical presentation was of primary septicemia, with positive cultures in both the blood and cutaneous lesions. stool cultures were positive for the organism in one patient with gastrointestinal symptoms. autopsy examination revealed liver cirrhosis in three cases and alcoholic liver disease in two; all showed portal hypertension. gastrointes ...	2002	12111206
the inherent characteristics and dna polymorphism of vibrio cholerae and other vibrios.	to investigate the inherent characteristics of vibrio cholerae (v. cholerae) and other vibrios and their relationship.	2002	12133305
wound infection due to vibrio vulnificus in spain.	vibrio vulnificus is a gram-negative rod that can cause septicaemia and skin lesions, usually in patients with underlying illnesses such as chronic liver disease or diabetes mellitus. infections caused by this bacterium are unusual in spain. a case of skin infection due to vibrio vulnificus is reported in a patient whose abraded skin on his left leg came into contact with seawater. the patient died suddenly, probably due to septicaemia or bacteraemia caused by this organism. vibrio vulnificus in ...	2002	12172745
vibrio vulnificus infections can be avoided.		2002	12183871
fulminant wound infections due to vibrio vulnificus.		2002	12183878
[basic studies on vibrio vulnificus infection: isolation of v. vulnificus from sea water, sea mud, and oysters].	to clarify the environmental distribution of vibrio vulnificus, sea water, sea mud, and oysters were examined at 13 sites, i.e. 4 sites in the tokyo bay (eastern japan) and 9 sites (5 sites for oysters) in tokushima prefecture (western japan). 1. v. vulnificus was isolated from 80 (54.8%) of the 146 samples of sea water examined. it was isolated from 19 (41.3%) of the 46 samples from western japan and 61 (61.0%) of the 100 samples from eastern japan. 2. it was isolated from 40 (40.8%) of the 98 ...	2002	12212318
cholesterol induce oligomerization of vibrio vulnificus cytolysin specifically.	vibrio vulnificus cytolysin (vvc) has been implicated as one of the important virulence determinants of v. vulnificus that causes serious septicemia and wound infection. an attempt was made to investigate that vvc could act as a ligand which stimulates intracellular signaling systems. cholesterol dose-dependently blocked vvc hemolytic activity through oli-gomerization of cytolysin. among cholesterol derivatives including 7-dehydrocholesterol, cholesteryl esters, deoxycholate, and cholestane test ...	2002	12216116
[vibrio vulnificus: an infrequent cause of septic shock].	vibrio vulnificus is a lactose positive gram negative rod that lives in warm seas and can infect wounds and produce sepsis. its infection is acquired after eating oysters or other filtering marine organisms. we report a 53 years old diabetic male who started with fever after a voyage to central america. he was admitted febrile, hypotense, dehydrated and polypneic. painful erythematous lesions and lumps were observed in his upper and lower limbs. after 72 hours of evolution, the lesions became vi ...	2002	12235904
an aeromonas salmonicida type iv pilin is required for virulence in rainbow trout oncorhynchus mykiss.	aeromonas salmonicida expresses a large number of proven and suspected virulence factors including bacterial surface proteins, extracellular degradative enzymes, and toxins. we report the isolation and characterization of a 4-gene cluster, tapabcd, from virulent a. salmonicida a450 that encodes proteins homologous to components required for type iv pilus biogenesis. one gene, tapa, encodes a protein with high homology to type iv pilus subunit proteins from many gram-negative bacterial pathogens, ...	2002	12240967
necrotising fasciitis caused by vibrio vulnificus in a child without known risk factors.		2002	12269260
infectious outbreaks associated with bivalve shellfish consumption: a worldwide perspective.	outbreaks of shellfish-associated infection have been reported for more than a century. since the early 1970s, the global consumption of shellfish has increased considerably--and with it, the reports of outbreaks of infection. most of these reports have originated from the united states, but europe and, to a lesser extent, asia and australia have also been represented. the majority of outbreaks have been linked to oysters, followed by clams and mussels. hepatitis a virus caused the largest ever ...	2002	12355378
promoter analysis and regulatory characteristics of vvhba encoding cytolytic hemolysin of vibrio vulnificus.	cytolytic hemolysin, a gene product of vvha, is a putative virulence factor of the pathogenic bacterium vibrio vulnificus. we have previously shown that hemolysin production is repressed by adding glucose to culture media and that production can be restored by adding camp. in this study, hemolysin activity and the level of vvh transcript were determined to reach a maximum in late exponential phase and were repressed when cells entered stationary phase. northern blot and primer extension analyses ...	2002	12356775
phage therapy of local and systemic disease caused by vibrio vulnificus in iron-dextran-treated mice.	vibrio vulnificus is a gram-negative bacterium that contaminates filter-feeding shellfish such as oysters. after ingestion of contaminated oysters, predisposed people may experience highly lethal septicemia. contamination of wounds with the bacteria can result in devastating necrotizing fasciitis, which can progress to septicemia. the extremely rapid progression of these diseases can render antibiotic treatment ineffective, and death is a frequent outcome. in this study, we examined the potentia ...	2002	12379704
in vitro and in vivo activities of newer fluoroquinolones against vibrio vulnificus.	the mics of six fluoroquinolones as well as minocycline and cefotaxime for 46 clinical isolates of vibrio vulnificus were determined by the agar dilution method. all the drugs tested had good activities against all isolates, with the mics at which 90% of the isolates tested were inhibited (mic(90)s) by five of the fluoroquinolones ranging between 0.03 and 0.06 micro g/ml. the mic(90) of lomefloxacin, on the other hand, was 0.12 micro g/ml. time-kill studies were conducted with these agents and a ...	2002	12384368
seasonality of chesapeake bay bacterioplankton species.	bacteria, gamma-subclass of proteobacteria, vibrio-photobacterium, vibrio vulnificus, vibrio cholerae-vibrio mimicus, and vibrio cincinnatiensis in water samples collected from the choptank river in chesapeake bay from 15 april to 16 december 1996 were enumerated using a fluorescent oligonucleotide direct-counting (fodc) procedure. fodc results obtained using a bacteria taxon-specific probe ranged from one-third the number of to the same number as that obtained by the acridine orange direct coun ...	2002	12406742
bacteria of the gamma-subclass proteobacteria associated with zooplankton in chesapeake bay.	the seasonal abundance of gamma-subclass proteobacteria, vibrio-photobacterium, vibrio cholerae-vibrio mimicus, vibrio cincinnatiensis, and vibrio vulnificus in the choptank river of chesapeake bay associated with zooplankton was monitored from april to december 1996. large (>202- microm) and small (64- to 202- microm) size classes of zooplankton were collected, and the bacteria associated with each of the zooplankton size classes were enumerated by fluorescent oligonucleotide direct count. larg ...	2002	12406743
detection of cytotoxin-hemolysin mrna in nonculturable populations of environmental and clinical vibrio vulnificus strains in artificial seawater.	the objective of this study was to develop a molecular detection method that better estimates the potential risk associated with the presence of vibrio vulnificus. for that purpose, we applied seminested reverse transcription-pcr (rt-pcr) to viable but nonculturable (vbnc) populations of v. vulnificus and targeted the cytotoxin-hemolysin virulence gene vvha. three strains, two environmental, if vv10 and if vv18, and one clinical, c7184, were used in this study. artificial seawater, inoculated wi ...	2002	12406760
induction of nitric oxide synthase expression by vibrio vulnificus cytolysin.	the pore-forming cytolysin of vibrio vulnificus (vvc) causes severe hypotension and vasodilatation in vivo. under the condition of bacterial sepsis, large amounts of nitric oxide (no) produced by inducible no synthase (inos) can contribute to host-induced tissue damage causing hypotension and septic shock. in this study, we investigated the effect of purified vvc on no production in mouse peritoneal macrophages. vvc induced no production in the presence of interferon-gamma. increased no producti ...	2002	11798187
unusual portal of entry of vibrio vulnificus: evidence of its prolonged survival on the skin.	vibrio vulnificus biotype 3 is an emerging pathogen isolated from cultures of skin and blood samples obtained from patients who were directly injured by the fins of tilapia fish bred in artificial ponds. we describe a patient infected with this pathogen after being punctured by a wire. meticulous anamnestic investigation demonstrated for the first time that this pathogen can survive on the skin for at least 24 hours.	2002	11807684
vibrio vulnificus and vibrio parahaemolyticus in u.s. retail shell oysters: a national survey from june 1998 to july 1999.	from june 1998 to july 1999, 370 lots of oysters in the shell were sampled at 275 different establishments (71%, restaurants or oyster bars; 27%, retail seafood markets: and 2%, wholesale seafood markets) in coastal and inland markets throughout the united states. the oysters were harvested from the gulf (49%). pacific (14%), mid-atlantic (18%), and north atlantic (11%) coasts of the united states and from canada (8%). densities of vibrio vulnificus and vibrio parahaemolyticus were determined us ...	2002	11808810
isolation and preliminary characterization of bacteriocins produced by vibrio vulnificus.	the aim of this work was to isolate bacteriocins from the environment that would be effective in neutralizing vibrio vulnificus in seafood.	2002	11849361
[an autopsied case of septicemia due to vibrio vulnificus].	a 63-year-old male with liver cirrhosis due to type-c hepatitis virus was admitted on june 14, 1999 to our hospital with complaints of dyspnea, and blisters, swelling and purpuras on his legs. he had consumed raw fish one or two days before. he was already in a state of shock with sepsis and disseminated intravascular coagulation shortly after the admission. although treatment with mepm and mino for sepsis, and daltepalin sodium, antithrombin iii and gabexate mesilate for disseminated intravascu ...	2002	11852476
vibrio vulnificus cytolysin induces hyperadhesiveness of pulmonary endothelial cells for neutrophils through endothelial p-selectin: a mechanism for pulmonary damage by vibrio vulnificus cytolysin.	vibrio vulnificus cytolysin forms transmembrane pores that are permeable to calcium ions in pulmonary endothelial cells, and has been suggested as an important virulence factor that sequestrate neutrophils primarily in the lung. to elucidate the mechanism we investigated whether the cytolysin affect the expression of endothelial p-selectin and adhesiveness of pulmonary endothelial cells for neutrophils. the cytolysin increased the adhesiveness of cpae cell, a pulmonary endothelial cell line, for ...	2002	12515397
sequence polymorphism of the 16s rrna gene of vibrio vulnificus is a possible indicator of strain virulence.	vibrio vulnificus exhibits considerable strain-to-strain variation in virulence. attempts to associate phenotypic or genotypic characteristics with strain virulence have been largely unsuccessful. based on a 17-nucleotide difference throughout the sequence of the small subunit 16s rrna gene, there are two major groups of v. vulnificus designated types a and b. in a survey of the 16s rrna genotype in 67 v. vulnificus human clinical and nonclinical strains, we determined that the majority of noncl ...	2003	12517889
use of diacetyl to reduce the load of vibrio vulnificus in the eastern oyster, crassostrea virginica.	vibrio vulnificus is a highly virulent human pathogen that occurs naturally among the microflora of oysters. this organism has two portals of entry into humans, one of which is ingestion. oysters containing v. vulnificus consumed in a raw or undercooked state often serve as a vehicle for the transmission of this organism. previous studies conducted in our laboratory have examined various generally recognized as safe compounds and have determined that diacetyl, a component of butter, is among the ...	2003	12540179
comparative genome analysis of vibrio vulnificus, a marine pathogen.	the halophile vibrio vulnificus is an etiologic agent of human mortality from seafood-borne infections. we applied whole-genome sequencing and comparative analysis to investigate the evolution of this pathogen. the genome of biotype 1 strain, v. vulnificus yj016, was sequenced and includes two chromosomes of estimated 3377 kbp and 1857 kbp in size, and a plasmid of 48,508 bp. a super-integron (si) was identified, and the si region spans 139 kbp and contains 188 gene cassettes. in contrast to non ...	2003	14656965
real-time pcr analysis of vibrio vulnificus from oysters.	vibrio vulnificus is an opportunistic human pathogen commonly found in estuarine environments. infections are associated with raw oyster consumption and can produce rapidly fatal septicemia in susceptible individuals. standard enumeration of this organism in shellfish or seawater is laborious and inaccurate; therefore, more efficient assays are needed. an oligonucleotide probe derived from the cytolysin gene, vvha, was previously used for colony hybridizations to enumerate v. vulnificus. however ...	2003	14660359
sensitivity of vibrio species in phosphate-buffered saline and in oysters to high-pressure processing.	multiple strains of vibrio vulnificus, vibrio parahaemolyticus, and vibrio cholerae non-o1 were tested in phosphate-buffered saline for their sensitivity to high-pressure processing (hpp). variability in sensitivity among strains was observed for all species; this variability decreased at higher pressures. v. vulnificus was the species that was most sensitive to treatment at 200 mpa (decimal reduction time [d] = 26 s), and v. cholerae was the species that was most resistant to treatment at 200 m ...	2003	14672224
abundance of potentially pathogenic micro-organisms in penaeus monodon larvae rearing systems in india.	monodon baculovirls (mbv), external fouling organisms (efo) and bacteria (especially vibrio species) were monitored during 1996-1997 at nine different penaeus monodon rearing hatcheries in india. total cultivable heterotrophic bacteria, vibrio-like-bacteria, presumptive vibrio harveyi, vibrio anguillarum, vibrio vulnificus counts were determined from shrimp eggs, post larvae, rearing tank water, source sea water, feed (artemia nauplii and microencapsulated feed). the mbv infected post larvae and ...	2003	14717450
vibrio vulnificus induces macrophage apoptosis in vitro and in vivo.	in this study, we compared the apoptotic activities of clinical and environmental isolates of vibrio vulnificus toward macrophages in vitro and in vivo. the clinical isolates induced apoptosis in macrophage-like cells in vitro and in macrophages in vivo. this suggests that macrophage apoptosis may be important for the clinical virulence of v. vulnificus.	2003	12496206
identification of genetic loci required for capsular expression in vibrio vulnificus.	transposon mutagenesis of an encapsulated, virulent strain of vibrio vulnificus 1003(o) led to the identification of four genetic regions that are essential to capsular polysaccharide (cps) expression and virulence. of the four regions, three are believed to be part of a capsule gene locus comprised of biosynthesis, polymerization, and transport genes clustered on a single chromosomal fragment. genes indicating a wzy-dependent system of polymerization and transmembrane export are present, sugges ...	2003	12595419
characterization of microbial communities from coastal waters using microarrays.	molecular methods, including dna probes, were used to identify and enumerate pathogenic vibrio species in the chesapeake bay; our data indicated that vibrio vulnificus exhibits seasonal fluctuations in number. our work included a characterization of total microbial communities from the bay; development of microarrays that identify and quantify the diversity of those communities; and observation of temporal changes in those communities. to identify members of the microbial community, we amplified ...	2003	12620025
histamine-releasing reaction induced by the n-terminal domain of vibrio vulnificus metalloprotease.	a zinc metalloprotease secreted by vibrio vulnificus, an opportunistic human pathogen causing septicemia and wound infection, stimulates exocytotic histamine release from rat mast cells. this protease consists of two functional domains: the n-terminal domain that catalyzes proteolytic reaction and the c-terminal domain that promotes the association with a protein substrate or cell membrane. like the intact protease, the n-terminal domain alone also induced histamine release from rat peritoneal m ...	2003	12628443
isolation of mutants of vibrio anguillarum defective in haeme utilisation and cloning of huva, a gene coding for an outer membrane protein involved in the use of haeme as iron source.	the isolation of vibrio anguillarum mutants lacking the ability to use haemin and haemoglobin as the only iron sources, as well as the identification of a gene involved in haeme utilisation are described. one of the isolated mutants defective in haeme utilisation lacked an iron-regulated outer membrane protein of 79-kda. although growth on haeme as iron source was completely abolished, the haemin and haemoglobin binding activities remained intact in the mutant, suggesting that the absent protein ...	2003	12647036
detection of pathogenic bacteria in shellfish using multiplex pcr followed by covalink nh microwell plate sandwich hybridization.	outbreak of diseases associated with consumption of raw shellfish especially oysters is a major concern to the seafood industry and public health agencies. a multiplex pcr amplification of targeted gene segments followed by dna-dna sandwich hybridization was optimized to detect the etiologic agents. first, a multiplex pcr amplification of hns, spvb, vvh, ctx and tl was developed enabling simultaneous detection of total salmonella enterica serotype typhimurium, vibrio vulnificus, vibrio cholerae ...	2003	12654491
[a life saving case of vibrio vulnificus (septic type)].	after eating raw shellfish, 62-year-old man suffered from watery diarrhea and leg pain. the disease developed to septicemia and necrotizing fasciitis of bilateral lower extremities. vibrio vulnificus was isolated from wound. he was treated with antibiotics, surgical debridement and hyperbaric oxygen therapy, and consequently was saved. effect of hyperbaric oxygen therapy on v. vulnificus infection was discussed.	2003	12708010
[a case of septicemia type vibrio vulnificus infection with necrotizing fasciitis rescued by lower extremity amputation].	we report a case of septicemia type vibrio vulnificus infection. the patient was a 74-year-old man who had liver cirrhosis and hepatocelluler carcinoma. he felt a pain in the right femoral lesion after eating raw shellfish (japanese "umitake") two days ago. he was admitted to our emergency center due to his shock status and thrombocytopenia two days after the onset. we diagnosed necrotizing fasciitis due to vibrio vulnificus infection, his life was saved by emergency amputation of the right lowe ...	2003	12708011
flagellar basal body flg operon as a virulence determinant of vibrio vulnificus.	vibrio vulnificus, a halophilic estuarine bacterium causing a rapidly progressing fatal septicemia, is highly cytotoxic to eukaryotic cells. to identify new virulence factors associated with cytotoxicity, we constructed a mariner-based transposon (tn himar1) library of the highly virulent clinical isolate mo6-24/o having a double mutation in the hemolysin and protease genes. the himar1 mutant library was extensively screened for the mutants showing decreased cytotoxicity to hela cells. we select ...	2003	12711330
[vibrios (vibrio cholerae, v. parahaemolyticus, v. vulnificus)].		2003	12718054
[noncholera vibrio infections (v. parahaemolyticus, v. vulnificus and others)].		2003	12718068
molecular characterisation and antimicrobial resistance of vibrio vulnificus and vibrio alginolyticus isolated from mussels (mytilus galloprovincialis).	twelve vibrio vulnificus biotype 1 and 11 vibrio alginolyticus isolated from mussels in italy were analysed by antimicrobial resistance, plasmid profiles, random amplification of polymorphic dna (rapd), and single enzyme amplified fragment length polymorphism (saflp). plasmid dna was detected in three v. vulnificus and four v. alginolyticus cultures. all isolates were resistant to at least two antimicrobial agents: all isolates were resistant to ampicillin, carbenicillin and streptomycin, except ...	2003	12747419
infections with vibrio vulnificus.	v. vulnificus is an uncommon cause of soft tissue infection and primary septicemia, especially in patients with hepatic disease or who patients who are immunocompromised. the mortality of infection in these patients is extremely high despite timely antibiotic therapy. it is important to consider the possibility of infection with v. vulnificus in patients who present with high fever and rapidly progressive sepsis and have a history of consumption of raw seafood (especially oysters) or exposure of ...	2003	12757246
health department liability for non-enforcement.		2003	12762123
response of vibrio parahaemolyticus 03:k6 to a hot water/cold shock pasteurization process.	vibrio vulnificus and v. parahaemolyticus are natural inhabitants of estuarine environments world wide. pathogenic strains of these bacteria are often transmitted to humans through consumption of raw oysters, which flourish in the same estuaries. previous studies reported the effective use of hot water pasteurization followed by cold shock to eliminate from raw oysters naturally and artificially incurred environmental strains of v. vulnificus and v. parahaemolyticus common to the gulf of mexico. ...	2003	12775474
vibrio vulnificus infection of the lower limb after fish spine injuries.	five patients with soft tissue infection of the lower extremity caused by vibrio vulnificus after penetrating injuries by fish spines are described. despite previously reported dismal rates of morbidity and mortality associated with vibrio septicemia, early measures including wide-spectrum intravenous antibiotics and surgical excision of devitalized tissues resulted in complete resolution in all cases. although penetrating injury is the common route of infection, indirect seeding may occur by th ...	2003	12782885
the kinetics of antibody production in mucus and serum of european eel (anguilla anguilla l.) after vaccination against vibrio vulnificus: development of a new method for antibody quantification in skin mucus.	vibrio vulnificus serovar e, a bacterial pathogen for eels cultured in intensive systems, is transmitted through water and enters into new hosts mainly via gills. the main objective of this work was to study the kinetics of antibody production to v. vulnificus in serum and mucus and their relationship with protection after vaccination. to quantify local mucus antibodies, a new "in situ" dot blot immunoassay using image analysis has been developed. this assay was applied to measure antibody produ ...	2003	12787687
intraspecific diversity of vibrio vulnificus in galveston bay water and oysters as determined by randomly amplified polymorphic dna pcr.	randomly amplified polymorphic dna (rapd) pcr was used to analyze the temporal and spatial intraspecific diversity of 208 vibrio vulnificus strains isolated from galveston bay water and oysters at five different sites between june 2000 and june 2001. v. vulnificus was not detected during the winter months (december through february). the densities of v. vulnificus in water and oysters were positively correlated with water temperature. cluster analysis of rapd pcr profiles of the 208 v. vulnificu ...	2003	12788713
population genetics of vibrio vulnificus: identification of two divisions and a distinct eel-pathogenic clone.	genetic relationships among 62 vibrio vulnificus strains of different geographical and host origins were analyzed by multilocus enzyme electrophoresis (mlee), random amplification of polymorphic dna (rapd), and sequence analyses of the reca and glna genes. out of 15 genetic loci analyzed by mlee, 11 were polymorphic. cluster analysis identified 43 distinct electrophoretic types (ets) separating the v. vulnificus population into two divisions (divisions i and ii). one et (et 35) included all indo ...	2003	12788717
ecology of vibrio vulnificus in estuarine waters of eastern north carolina.	while several studies on the ecology of vibrio vulnificus in gulf coast environments have been reported, there is little information on the distribution of this pathogen in east coast waters. thus, we conducted a multiyear study on the ecology of v. vulnificus in estuarine waters of the eastern united states, employing extensive multiple regression analyses to reveal the major environmental factors controlling the presence of this pathogen, and of vibrio spp., in these environments. monthly fiel ...	2003	12788759