Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| a reappraisal of the complement fixation test using soluble mycobacterium avium antigen for the detection of m. paratuberculosis infection in cattle. | serums from 263 cattle suspected of having paratuberculosis on the basis of clinical signs, were tested for antibodies to mycobacterium paratuberculosis with a complement fixation test (cft) employing a heat extracted, soluble m. avium antigen. microscopic examination confirmed that 172 (65.4%) clinically affected animals had paratuberculosis, the remainder being disease-free. the specificity and sensitivity of the cft was 92.3% and 74.4% respectively. phenol treatment of serums before testing w ... | 1986 | 3753338 |
| cartilage-inducing factor-a. apparent identity to transforming growth factor-beta. | comparison of the sequence of the n-terminal 30 amino acids of cartilage-inducing factor-a (cif-a) from bovine demineralized bone with the corresponding sequence of human transforming growth factor-beta (tgf-beta) revealed 100% identity. furthermore, cif-a stimulated normal rat kidney fibroblasts to become anchorage-independent and form colonies in soft agar (in the presence of epidermal growth factor) in a manner similar to tgf-beta. similarly, tgf-beta from human platelets induced rat muscle m ... | 1986 | 3754555 |
| cadmium toxicity in kidney cells. resistance induced by short term pretreatment in vitro and in vivo. | epithelial cells from the kidney were freshly isolated from rats pretreated by daily subcutaneous doses of cdcl2 in vivo (0.5-2 mg cd/kg x 5). such cells were incubated in vitro in media with different concentrations of cadmium chloride (0-200 micrograms cd/ml). there was no inhibition of cell growth in such cells. however, in cells isolated from non-treated rats, in vitro exposure to the same concentrations of cdcl2 caused a dose dependent decrease in viability. when cells, isolated from non-tr ... | 1986 | 3754681 |
| stabilization of intracellular recordings in the cat spinal cord using high-frequency ventilation. | stabilization of intracellular recordings by a substantial reduction of respiratory motion may be accomplished by the use of high-frequency ventilation (600-800 breaths/min). the technique is a simple one and enables maintenance of relatively constant blood gas tensions. although the improved stability was demonstrated in experiments performed on alpha motoneurones in the thoracic spinal cord, it is anticipated that a reduced respiratory movement should benefit recordings in other spinal and sup ... | 1986 | 3756629 |
| the effect of c1 spinal cord transection or bilateral adrenal vein ligation on thioridazine-induced arrhythmia and death in the cat. | the phenothiazine thioridazine 1 mg/kg/min was infused intravenously into three groups of cats: (1) thioridazine alone (n = 5), (2) after bilateral adrenal ligation (n = 4), and (3) after spinal cord section at the atlanto-occipital junction (c1; n = 6). the times to arrhythmia and death with thioridazine alone were 47.8 +/- 7.8 and 72.8 +/- 5.6 minutes respectively. after bilateral adrenal ligation, arrhythmia and death occurred at 41.1 +/- 5.2 and 53.1 +/- 5.8 minutes, respectively, which show ... | 1986 | 3760244 |
| [hemodynamic effects of naloxone in man in brain death]. | 1986 | 3766888 | |
| evaluation of methods for enumeration of vibrio parahaemolyticus from seafood. | the efficiency of several enrichment broths in recovering vibrio parahaemolyticus inoculated into fish homogenates was studied. recovery by the most probable number technique was very low in all the broths, while direct plating on thiosulfate citrate bile salt sucrose agar yielded better recovery. a decrease in the enrichment time to 8 from 18 h did not improve recovery. at concentrations exceeding 2.5 micrograms/ml, polymyxin was inhibitory to v. parahaemolyticus. | 1986 | 3767361 |
| functional coupling between transient declines in blood glucose and feeding behavior: temporal relationships. | to assess the strength and time course of the functional coupling between transient declines in blood glucose and meal initiation, access to food was prevented throughout declines followed by restoration of access to food when glucose returned to baseline. neither preventing access to nor the absence of food affected the time course of the decline in blood glucose and the latency to food seeking behavior. when access to food was restored six to eight minutes after the blood glucose returned to b ... | 1986 | 3768746 |
| [regeneration of changes induced by a single dose of bromhexine in the epithelium of the respiratory tract]. | 1986 | 3769026 | |
| role of glucagon in splanchnic hyperemia of chronic portal hypertension. | the role of glucagon as a blood-borne mediator of the hyperdynamic circulation associated with chronic portal venous hypertension was assessed in the rat portal vein stenosis model. selective removal of pancreatic glucagon from the circulation was achieved by intravenous infusion of a highly specific glucagon antiserum. blood flow to splanchnic organs, kidneys, and testicles was measured with radioactive microspheres, and the reference-sample method. glucagon antiserum had no effect on blood flo ... | 1986 | 3777172 |
| participation of iron on the growth inhibition of pathogenic strains of mycobacterium avium and m. paratuberculosis in serum. | serum, which is low in freely-available iron, was bacteriostatic for m. avium and m. paratuberculosis irrespective of the ability of these organisms to produce the iron-chelators mycobactin and exochelin for iron acquisition. bacteriostasis was overcome when the serum was supplemented with exochelin or mycobactin, but only if the mycobacteria had previously adapted to growth in a low iron environment. the results imply that for studies on mycobacterial pathogenicity the test cultures should be g ... | 1986 | 3788345 |
| occurrence and distribution of halophilic vibrios in subtropical coastal waters of hong kong. | the summer occurrence and distribution of halophilic vibrios in the subtropical coastal waters of hong kong were investigated. the density of vibrios in six sample sites ranged from 90 to 6,700 per ml, which made up 0.41 to 40% of the total bacterial populations of these sample sites. the sucrose-positive vibrios were found to be much more common (88% of total vibrios) than the sucrose-negative ones. a total of 48 strains belonging to six vibrio species were fully characterized. among these, vib ... | 1986 | 3789725 |
| low-density lipoprotein apheresis and regression of atherosclerotic plaque in vitro. | short-term organ culture and primary cell culture of human aorta were used to study the effect of selective removal of low-density lipoproteins (ldls) from the surrounding medium (ldl apheresis) on the lipid content of cultured tissue and cells, respectively. ldl apheresis was performed by passing the culture medium through immunosorbent containing agarose-bound goat antibodies against human ldl. ldl apheresis promoted the decrease of lipids of all classes in the cultured atherosclerotic plaque. ... | 1986 | 3800702 |
| furosemide lowers intracranial pressure by inhibiting csf production. | furosemide administration effectively lowers intracranial pressure in newborn preterm and term rabbit pups. this effect may be due to the diuretic action of the drug, its ability to inhibit cerebrospinal fluid production or to a combination of both. to test these possibilities newborn rabbits were either injected with furosemide and left unmolested for 6 hours, or anaesthetized and subjected to ventriculocisternal perfusions. during the 6 hour postnatal period the decrease in body weight was 8 t ... | 1986 | 3811613 |
| ultrastructural changes in the sarcoplasmic reticulum in acute myocardial ischemia. | we investigated the morphologic changes in the sarcoplasmic reticulum (sr) in acute myocardial ischemia, induced by occlusion of the coronary artery in the canine heart, by freeze-fracture of sr in situ and in vitro, and they were compared to the alterations in ca++-stimulated atpase activity and protein composition of the isolated sr. both sr in situ and the isolated sr exhibited typical intramembranous particles with diameters of 70 to 90 a in freeze-fracture replicas. the intramembranous part ... | 1986 | 2948034 |
| the iridovirus frog virus 3: a model for trans-acting proteins. | the amphibian iridovirus, frog virus 3 (fv3), produces at least two trans-activating proteins that stimulate the expression of viral genes. one of these proteins induces transcription from the promoter of an immediate-early fv3 gene, whereas the other induces transcription from exogenously methylated dna. these proteins may serve as a model for both viral and cellular trans-acting factors. | 1986 | 3153144 |
| [dna-dna homology and phenotypic characterization of environmental strains of vibrio parahaemolyticus and vibrio pelagius]. | a taxonomic study by means of dna homology and the most recent phenetic criteria has been carried out in 11 environmental strains of vibrio parahaemolyticus and 4 of vibrio pelagius, previously ascribed to the respective phenons in a numerical taxonomy study. the strains of v. parahaemolyticus showed genetic homogeneity, a guanine plus cytosine content (g + c) of 46% and 61% homology with v. parahaemolyticus type strain attc 17802. they exhibited phenetic atypicalities but could be ascribed to t ... | 1986 | 3271060 |
| identification of an autonomously replicating sequence near a histone gene of physarum polycephalum. | fragments of dna which function as autonomous replication sequences in yeast were cloned from physarum polycephalum. the ars activity is located in a 1.2 kbp fragment extending 1.5 kbp to 2.7 kbp upstream of the 5' end of a histone h4 gene. our recent finding that a replication origin is located at a distance less than 3 kbp of this histone gene suggests that the ars element identified coincides with a specialized replication origin and can be used to direct chromosome replication in physarum po ... | 1986 | 3442825 |
| [human gingival pigmentation due to electrochemical degradation. immunological study of biopsies using monoclonal antibodies]. | 1986 | 3464603 | |
| comparison of the nucleotide sequences of the genes for the thermostable direct hemolysin and the thermolabile hemolysin from vibrio parahaemolyticus. | the nucleotide sequences of genes encoding the thermostable direct (tsd) hemolysin and the thermolabile (tl) hemolysin of vibrio parahaemolyticus were determined. from the nucleotide sequence of the tsd hemolysin gene, it was revealed that the preprotein and the mature protein consisted of 189 amino acids and 165 amino acids, and that the molecular weights were 21.1 kda or 18.5 kda, respectively. our data regarding tsd hemolysin were in complete agreement with previously published data. from the ... | 1986 | 3508495 |
| bacterial diarrhoea. | bacterial infections are important causes of diarrhoea in infants and children, particularly in developing countries and in other settings where standards of personal and community hygiene are low. knowledge of bacterial diarrhoeas has been significantly expanded in recent years by the finding that many episodes of acute diarrhoea are due to infections with bacteria which produce enterotoxins that interfere with intestinal fluid and electrolyte transport (the 'enterotoxigenic' diarrhoeas). sever ... | 1986 | 3514008 |
| effect of 1,25-dihydroxyvitamin d3 on insulin secretion: direct or mediated? | in order to clarify the role of vitamin d (d) in regulating insulin secretion, we studied the effect of long term (10 days) and short term (3 days) supplementation with d and/or calcium (ca) on insulin secretion from the isolated, perfused pancreas of d- and ca-deficient rats. the influence of the nutritional state induced by d deficiency was also evaluated. the long term supplementation of either d, ca, or both restored the body weight and improved insulin secretion induced by high glucose conc ... | 1986 | 3516655 |
| feasibility of same-day identification of members of the family vibrionaceae by the api 20e system. | sixty isolates, comprising nine species of the family vibrionaceae, were tested with the api 20e 5-h same-day procedure (analytab products, plainview, n.y.). included were 27 aeromonas hydrophila isolates, 10 aeromonas sobria isolates, 7 aeromonas caviae isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolate. the 5-h profile numbers were specific fo ... | 1986 | 3517061 |
| guanidine hydrochloride denaturation studies of mutant forms of staphylococcal nuclease. | several mutant forms of staphylococcal nuclease with one or two defined amino acid substitutions have been purified, and the effects of the altered amino acid sequence on the stability of the folded conformation have been analyzed by guanidine hydrochloride denaturation. two nuc- mutations, which greatly reduced the level of enzyme activity accumulated in e coli colonies carrying a recombinant plasmid with the mutant nuc gene (ie, a nuc- phenotype), both result in protein unfolding at significan ... | 1986 | 3519625 |
| natural killer and t-cell potentiation by monoclonal igg against natural killer cell fcr(igg) or the t3 complex. | treatment of human natural killer (nk) cells with monoclonal antibodies of the igg isotype against nk cell-fcr(igg) increased lysis of most haematopoietic target cell lines with high or intermediate background nk susceptibility. treatment of normal non-adherent lymphocytes with an igg anti-t3 monoclonal antibody also increased lysis against the same target cells. potentiating anti-fcr antibodies rapidly modulated fcr activity and the capacity of the cells to act as antibody-dependent killers, al ... | 1986 | 3520802 |
| production of extracellular enzymes and cytotoxicity by vibrio vulnificus. | thirty-three strains of vibrio vulnificus of clinical and environmental origin were examined for production of 12 extracellular enzymes of potential importance to the virulence of this bacterium. strains of vibrio vulnificus were consistent in their production of protease, mucinase, lipase, chondroitinase, hyaluronidase, dnase, sulfatase, and hemolysin. no differences between clinical and environmental isolates were noted. although none of the enzymes appeared to correlate with the ability of th ... | 1986 | 3522090 |
| etiology and epidemiology of travelers' diarrhea in asia. | travelers' diarrhea in asia has been studied among peace corps volunteers in thailand, japanese travelers, foreign residents in bangladesh, guests in hotels, and members of various tour groups. rates of diarrheal attack of greater than 50% during four- to six-week sojourns were reported for these groups. among travelers with diarrhea, the most commonly isolated pathogen was enterotoxigenic escherichia coli (20%-34%), followed by salmonella (11%-15%), shigella (4%-7%), campylobacter (2%-5%), and ... | 1986 | 3523709 |
| reversible and irreversible neuronal damage caused by excitatory amino acid analogues in rat cerebellar slices. | slice preparations of the developing rat cerebellum were used to investigate the light and electron microscopic correlates of reversible and irreversible neuronal injury caused by the neurotoxic excitatory amino acid receptor agonists, kainate and n-methyl-d-aspartate. the slices were examined after various periods of exposure to the agonists (up to 30 min) with or without a 90 min recovery period in agonist-free medium. n-methyl-d-aspartate (100 microm) caused necrosis of deep nuclear neurons a ... | 1986 | 3526173 |
| reversible and irreversible neuronal damage caused by excitatory amino acid analogues in rat cerebellar slices. | slice preparations of the developing rat cerebellum were used to investigate the light and electron microscopic correlates of reversible and irreversible neuronal injury caused by the neurotoxic excitatory amino acid receptor agonists, kainate and n-methyl-d-aspartate. the slices were examined after various periods of exposure to the agonists (up to 30 min) with or without a 90 min recovery period in agonist-free medium. n-methyl-d-aspartate (100 microm) caused necrosis of deep nuclear neurons a ... | 1986 | 3526173 |
| [effect of dichroa febrifuga l. on chloroquinsensible and chloroquinresistant malaria parasites]. | 1986 | 3528514 | |
| mechanisms of plasmid-mediated antibiotic resistances in vibrio parahaemolyticus. | the mechanisms of drug resistance of clinical isolate, vibrio (v.) parahaemolyticus st550, resistant to chloramphenicol (cp), aminoglycoside antibiotics (ags) and beta-lactam antibiotics were investigated. the mechanisms of resistance to cp, ags and beta-lactam antibiotics were dependent on chloramphenicol acetyltransferase (cat), aminoglycoside-3"-adenylyltransferase aad(3") and aminoglycoside-3'-phosphotransferase aph(3') and tem type penicillinase, respectively. | 1986 | 3528768 |
| the immunohistochemical detection of lymph node metastases from infiltrating lobular carcinoma of the breast. | immunological markers improve specificity and accuracy of cell detection, therefore it is important to evaluate their usefulness in improving standard histological procedures. this study investigates whether immunocytochemical techniques increase the accuracy of detection, in axillary lymph nodes, of metastatic cells from infiltrating breast lobular carcinoma (ilc). fifty cases of ilc reported to be node-negative were selected. new serial sections were cut from a total of 767 lymph nodes, staine ... | 1986 | 3535864 |
| experience with isoxicam and catabolin. | 1 synovial cells produce a protein factor, catabolin, that induces chondrocytes to resorb their own matrix with the release of glycosaminoglycan (gag). 2 studies were performed to determine the effect of isoxicam, a new nsaid, on gag production in a catabolin system utilizing tissue culture of bovine nasal septum in a medium containing pooled synovial tissue from young pigs. 3 using fresh medium containing no synovial tissue, the release of gag by inherent cartilage breakdown was 49.7% after 8 d ... | 1986 | 3497659 |
| management of opportunistic infections complicating the acquired immunodeficiency syndrome. | therapy of opportunistic infection in patients with the acquired immunodeficiency syndrome is frustrating, and there is no convincing evidence that aggressive treatment and/or prophylaxis other than for pneumocystis infection can significantly prolong life. while much clinical effort is expended on treating sequential life-threatening infections, the overall course is usually progressively downhill. thus, any real impact on the disease should be aimed at the causative viral agent, because it is ... | 1986 | 3485749 |
| isotope exchange at equilibrium studies with rat muscle adenylosuccinate synthetase. | the kinetic mechanism of rat muscle adenylosuccinate synthetase was studied by determining the rates of isotope exchange at equilibrium. a random sequential binding mechanism was indicated for both the forward and reverse reactions. aspartate, adenylosuccinate, gdp, and pi were determined to bind in rapid equilibrium. gtp exchanges with both gdp and pi at the same rate, which is similar to the exchange rate of imp with adenylosuccinate. aspartate exchanges with adenylosuccinate at a higher rate ... | 1986 | 3542024 |
| consequences of treatment of gastrointestinal infections. | diarrhoeal disease is a common problem in developing countries. as a result of recent advances in diagnostic methodology, the causative agents can now be identified in most cases of acute diarrhoeal diseases. enteric bacterial pathogens are the common cause of gastroenteritis in developing countries. appropriate uses of antibiotics in selected cases of diarrhoea will decrease symptoms or reduce faecal shedding of the organism and prevent spread of infection. antimicrobial agents improve the diar ... | 1986 | 3547620 |
| degenerative lesions of a coronary chemoreceptor and nearby neural elements in the hearts of victims of sudden death. | a coronary chemoreceptor and its neighboring nerves and ganglia were studied by serial sections of tissue taken from the hearts of sixteen victims of sudden unexpected death. either focal or extensive inflammatory destruction was present in the chemoreceptors of 13 of the 16 hearts, and similar abnormalities of local nerves and ganglia were present in all 16 hearts. since an identical coronary chemoreceptor in the dog has previously been shown to be the site of origin of a powerful reflex with m ... | 1986 | 3837961 |
| the primary structure of the hemoglobin of the european souslik (citellus citellus, rodentia). | the complete primary structures of two hemoglobin components of the european souslik (citellus citellus) are presented. the two hemoglobins have identical alpha-chains but differ in the amino-acid sequence of their beta-chains. the chain separation was achieved by chromatography on carboxymethyl-cellulose cm-52. amino-acid sequences were established by automatic liquid-phase and gas-phase edman degradation of the globin chains, of their tryptic peptides and of a peptide resulting from acidic hyd ... | 1986 | 3814354 |
| role of chitin in the survival of vibrio parahaemolyticus at different temperatures. | washed cells of vibrio parahaemolyticus declined in numbers when incubated in phosphate-buffered saline for 6 h at different temperatures. addition of chitin flakes to phosphate-buffered saline not only helped the organism to survive, but also resulted in an increase in cell numbers, particularly at 10 degrees c. the effect of chitin could not be simulated by n-acetylglucosamine, yeast extract, starch, or casein. | 1986 | 3815158 |
| evaluation of the hereditary syrian hamster cardiomyopathy by 31p nuclear magnetic resonance spectroscopy: improvement after acute verapamil therapy. | the relation between metabolic and functional derangement in various cardiomyopathies has not been well characterized. this information was specifically sought in a spontaneous cardiomyopathic model. metabolic and hemodynamic parameters were obtained in glucose-perfused beating hearts of 180-200-day-old cardiomyopathic syrian hamsters and age-matched healthy animals. this period in the cardiomyopathic hamster lifetime is intermediary between the necrotic phase and the appearance of heart failure ... | 1986 | 3815756 |
| [neuronal organization of conditioned inhibition]. | 1986 | 3817182 | |
| processing-independent in vitro translocation of cytochrome p-450(scc) precursor across mitochondrial membranes. | in the presence of a membrane-permeable metal chelator, bovine adrenal cortex mitochondria imported p-450(scc) precursor without processing of the amino-terminal extension peptide. the imported precursor was bound to the matrix side surface of the inner membrane. when the inhibition due to the metal chelator was removed, the imported precursor was processed to the mature form. unprocessed precursor was also detected in mitochondria when the import reaction was carried out at relatively low tempe ... | 1986 | 3818579 |
| similarity principles and intrinsic geometries: contrasting approaches to interspecies scaling. | we criticize standard allometric approaches on the grounds that they emphasize scaling to one variable at a time, whereas chemically reactive hydrodynamic systems involved in pharmacokinetic phenomena are of higher dimension. we show that attempts based on mechanical similitude to set a dosage that would be equivalent across species (for example, from mouse to humans) lead to ambiguous results. another failing of standard allometry may be its incapability to accommodate the neoteny of homo sapie ... | 1986 | 3820095 |
| role of common house flies in transmission of v. parahaemolyticus infection in calcutta slum in relation to carrier rate of the said pathogen. | 1986 | 3610299 | |
| comparative evaluation of identification systems for testing methicillin-resistant strains of staphylococcus aureus. | several commercial systems are available to distinguish between staphylococcus aureus and the coagulase-negative species of the micrococcaceae family. four latex agglutination systems (accu-staph, serostat, staphaurex, and staphylatex) and two hemagglutination systems (hemastaph and staphyloslide) were compared for their performance in the rapid identification of 232 isolates of staphylococci, including 114 of methicillin-resistant s. aureus. accu-staph, staphaurex, and staphyloslide correctly i ... | 1986 | 3638308 |
| video display terminals--how they affect the health of clerical workers. | 1987 | 3647778 | |
| [contamination of seafood by vibrio parahaemolyticus in taiwan]. | from october 1984 to september 1985, a total of 770 seafood samples, collected from the retail markets of 8 coastal cities in taiwan, were tested for the contamination of vibrio parahaemolyticus. the results showed that 352 samples (45.7%) were contaminated by v. parahaemolyticus. the detection frequencies of various samples were as follows: 40.0% fish samples, 22.3% fish fillets, 44.4% shrimps and 47.8% crabs of the crustacea group, 68.7% bivalve shellfish and 31.9% non-bivalve shellfish of the ... | 1987 | 3652783 |
| the effect of corneal contact lenses on the oxygen tension in the anterior chamber of the rabbit eye. | the oxygen tension in the aqueous humor in the anterior chamber of rabbit eyes was measured continuously with a polarographic electrode. the normal oxygen tension in the anterior chamber was 23 +/- 2 mm hg (mean +/- sd, n = 4). a contact lens was then placed on the cornea for at least 10 min and the drop in oxygen tension recorded. a hard polymethylmethacrylate lens reduced the oxygen tension by 16 +/- 4 mm hg, and a larger hydroxyethylmethacrylate soft lens (soflens) decreased oxygen tension by ... | 1987 | 3654146 |
| the l- and r-type isozymes of rat pyruvate kinase are produced from a single gene by use of different promoters. | cdna clones for rat r-type pyruvate kinase and a genomic clone encoding both l- and r-type isozyme mrnas were isolated. their sequences were compared with that of the l-type isozyme cdna to determine the sequences of mrna and protein of the r-type isozyme and the organizations of the l- and r-type genes. results showed that the r-type isozyme mrna had an identical nucleotide sequence to that of the l-type except in the 5'-terminal region including the coding sequence and the length of the 3'-unt ... | 1987 | 3654663 |
| synthesis of 7a-substituted benzoylaminoalkyl-hexahydro-1h-pyrrolizines and evaluation of their antiarrhythmic activity. | several 7a-substituted benzoylaminoalkyl-hexahydro-1 h-pyrrolizines were synthesized by acylations of 7a-aminoalkyl-hexahydro-1 h-pyrrolizines. all compounds synthesized were evaluated for their antiarrhythmic activities using the chloroform-mouse method, and some of them were found to have significant antiarrhythmic activities, comparable to that of procainamide. | 1987 | 3656104 |
| endogenous bursting by rat supraoptic neuroendocrine cells is calcium dependent. | 1. phasic bursting by magnocellular neuroendocrine cells (m.n.c.s) in vivo causes increased vasopressin release from axon terminals in the neurohypophysis. in the supraoptic nucleus of the coronal hypothalamic slice thirty-two of sixty-five m.n.c.s recorded intracellularly displayed repetitive bursting, either spontaneously or during a low level of tonic current injection. 2. of the thirty-two repetitive bursters, twenty-four received no apparent patterned synaptic input and the phasic burst beh ... | 1987 | 3656152 |
| metabolic fate in the dog of the nitroxide moiety in a compound with potential utility as a contrast agent in mri. | nitroxides, paramagnetic compounds with demonstrated effectiveness as contrast agents in proton magnetic resonance imaging, shorten the relaxation times of protons and therefore cause an increase in image intensity in tissues into which they distribute. in this study, the metabolic fate of the nitroxide moiety was examined in the dog using a pyrrolidine nitroxide derivative, 2,2,5,5-tetramethylpyrrolidine-1-oxyl-3-carboxylic acid, for which contrast-enhancing properties have been previously stud ... | 1987 | 3657496 |
| metabolic fate in the dog of the nitroxide moiety in a compound with potential utility as a contrast agent in mri. | nitroxides, paramagnetic compounds with demonstrated effectiveness as contrast agents in proton magnetic resonance imaging, shorten the relaxation times of protons and therefore cause an increase in image intensity in tissues into which they distribute. in this study, the metabolic fate of the nitroxide moiety was examined in the dog using a pyrrolidine nitroxide derivative, 2,2,5,5-tetramethylpyrrolidine-1-oxyl-3-carboxylic acid, for which contrast-enhancing properties have been previously stud ... | 1987 | 3657496 |
| na+ modulates the k+ permeability and the membrane potential of alkalophilic bacillus. | in the absence of na+ in the medium, the membrane potential of obligately alkalophilic bacillus cells was found to be decreased by the addition of k+ to the medium, whereas k+ addition in the presence of na+ had no effect. rb+ showed essentially the same effect as k+. the decreased membrane potential was quickly restored by lowering the k+ concentration in the medium or by adding na+ or li+ to the medium. thus, in the absence of na+, the membrane potential of alkalophilic bacillus seems to be af ... | 1987 | 3663674 |
| liposome as an adjuvant for the production of estradiol antibodies. | mice immunized with liposome associated estradiol-bovine serum albumin conjugate (e2-bsa) showed a significantly higher estradiol-specific immune response than did mice injected with the free conjugate. the conjugation of estradiol with bsa is imperative, since injection of liposomes with estradiol in the lipid bilayer was found to be ineffective in inducing an immune response. entrapment of e2-bsa in neutral and positively charged liposomes was found to be a potent mode of immunostimulation, ev ... | 1987 | 3666803 |
| crystal violet ring response as a marker for in vitro detection of pathogenic vibrio parahaemolyticus & shigella spp. | 1987 | 3666851 | |
| [roentgeno-morphological characteristics of post-traumatic deformities in children]. | 1987 | 3670811 | |
| acute diarrhoea in adults: a prospective study. | a prospective study of 74 adults with acute diarrhoea was carried out in sydney in 1984-1985 to determine the infective agents that were involved and their relationship to clinical, epidemiological and laboratory features. thirty-four potential pathogens were identified in 32 (43.2%) patients. these included, in order of frequency: campylobacter spp., rotavirus, clostridium difficile, salmonella enteritidis, aeromonas hydrophila, vibrio parahaemolyticus, adenovirus, a small round virus and giard ... | 1987 | 3683260 |
| age-dependent activation of glucocorticoid receptors in the cerebral hemispheres of male rats. | the binding of [3h]dexamethasone-receptor complexes to purified nuclei was studied in the cerebral hemispheres of immature (3-week-old) and mature (26-week-old) long-evans male rats to determine the age-related changes, if any, in the physicochemical properties of glucocorticoid receptors. our data show that heat activation (for 45 min at 25 degrees c) significantly enhances the nuclear binding of [3h]dexamethasone-receptor complexes in rats of both ages, with a greater magnitude in immature rat ... | 1987 | 3690338 |
| [evaluation of cefuzonam in the pediatric field]. | cefuzonam (l-105, czon) was studied in pediatric infections. a summary of the results it as follows: for recently isolated staphylococcus aureus strains, peak mics of czon were distributed between 0.39 and 0.78 micrograms/ml showing a greater susceptibility of s. aureus to czon than to cefoperazone (cpz), latamoxef (lmox), and cefmenoxime (cmx). peak mics of czon for escherichia coli were 0.10-0.20 micrograms/ml, similar to those of cpz, lmox, and cmx. ampicillin (abpc)-resistant strains were al ... | 1987 | 3613082 |
| pentapeptide nuclear localization signal in adenovirus e1a. | the adenovirus e1a gene products are nuclear proteins important in transcriptional control of viral functions during infection. by producing normal e1a proteins and derivatives of e1a in bacteria and microinjecting these proteins into cultured cells, we were able to examine their ability to localize to the nucleus. we showed that a short peptide sequence at the carboxyl terminus of e1a is necessary for the rapid (30-min) nuclear localization of that protein. additionally, we showed that just the ... | 1987 | 3614197 |
| [ideal experimental microsurgical methods in vasovasostomy and vasoepididymostomy]. | the vasovasostomy with the double-layered end-to-end anastomosis by silber is a microsurgical ideal method. we think we found experimentally an ideal method for the vasoepididymostomy with the open end-to-side anastomosis and direct spermato-tight microsurgical suture. specialties of the species rat and dog have to be observed. restrictions of the anastomotic region lead to infertility. | 1987 | 3617849 |
| colorimetric assay for methanesulfinic acid in biological samples. | we describe a simple colorimetric method to measure 30 to 300 microm concentrations of sulfinic acids in biologic samples. the procedure employs the coupling reaction of an aromatic diazonium salt (ar--n = n+) with the sulfinic acids (rsooh) to produce a colored diazosulfone derivative (ar-n = n-soor), which can be selectively extracted into an organic solvent. linearity as well as noninterference by liver homogenate, phenols, amines, and thousandfold or greater excesses of sulfate, thiol, and d ... | 1987 | 3619031 |
| [antibiotic prophylaxis in surgery. biological basis for the use of 2d-generation cephalosporins]. | 1987 | 3619351 | |
| [a study of serological typing distribution on vibrio parahaemolyticus along the off-shore of china]. | 1987 | 3621317 | |
| fluorometric detection of 2-amino-3-methylimidazo[4,5-f]quinoline, 2-amino-3,4-dimethylimidazo[4,5-f]quinoline and their n-acetylated metabolites excreted by the rat. | a sensitive fluorometric method has been developed for the determination of carcinogenic amino imidazoazaarenes (aia compounds) which are formed during the cooking of food. they are separated by t.l.c. followed by spraying with dimethylsulfoxide and visualization by long-wave u.v. light. quantification is done by fluorescence scanning. this method was applied to the determination of the metabolites in urine and feces from rats given 5 mg/kg body wt i.p. of 2-amino-3-methylimidazo[4,5-f]quinoline ... | 1987 | 3621465 |
| defective biosynthesis of proteolipid protein in pelizaeus-merzbacher disease. | the brain of an 18-year-old patient with pelizaeus-merzbacher disease was examined by standard neuropathological and biochemical methods and by immunocytochemical and immunochemical techniques. analysis revealed a lack of myelin-specific lipids, but showed a residual immunoreactivity for myelin basic protein, myelin-associated glycoprotein, and 2',3'-cyclic nucleotide-3'-phosphodiesterase. examination by immunocytochemistry and enzyme-linked immunosorbent assay showed an absence of proteolipid a ... | 1987 | 3827224 |
| adult respiratory distress syndrome. a live e coli septic primate model. | 1987 | 3548404 | |
| the morphology and distribution of serotonin-like immunoreactive fibers in the cat dorsal column nuclei. | fibers showing serotonin-like immunoreactivity (5-ht-li) are demonstrated in the gracile, cuneate and external cuneate nuclei of the cat using avidin-biotinylated horseradish peroxidase and indirect fluorescence immunohistochemical methods. 5-ht-li fibers are located in all cytoarchitectural subdivisions of the gracile and cuneate nuclei but are restricted to the medial half of the external cuneate nucleus. in all nuclei, 5-ht-li fibers consist of long, varicose strands showing a wide range in t ... | 1987 | 3554006 |
| calcitonin gene-related peptide in the ventral tegmental area: selective modulation of prefrontal cortical dopamine metabolism. | the distribution of calcitonin gene-related peptide (cgrp) in the a10 dopamine (da) cell group region of the ventral tegmental area (vta) of the rat was examined using immunohistochemical techniques. cgrp-like immunoreactivity was localized to axons innervating the rostral and dorsal vta. direct administration of cgrp to the vta of the rat resulted in a dose-related increase in da utilization in the medial prefrontal cortex, but not other mesocortical, mesolimbic, or striatal da terminal field r ... | 1987 | 3554009 |
| restorative dentistry. | various restorative methods like composite crowns, parapulpar pin reconstructions or vast crowns and bridges, are evaluated against each other. also new methods for repair of jaw-fractures in dogs and cats by means of parapulpar pins and composites are discussed. | 1987 | 3554609 |
| ranking possible carcinogenic hazards. | this review discusses reasons why animal cancer tests cannot be used to predict absolute human risks. such tests, however, may be used to indicate that some chemicals might be of greater concern than others. possible hazards to humans from a variety of rodent carcinogens are ranked by an index that relates the potency of each carcinogen in rodents to the exposure in humans. this ranking suggests that carcinogenic hazards from current levels of pesticide residues or water pollution are likely to ... | 1987 | 3563506 |
| decreased sialic acid content of erythrocytes in patients with aplastic anaemia. | the sialic acid content of erythrocytes from patients with aplastic anaemia was determined and compared with those in patients with several haematological disorders and healthy individuals. the sialic acid was released enzymatically with vibrio cholerae sialidase and quantitated by the thiobarbituric acid method (aminoff, 1961). the sialic acid content of normal erythrocytes was 538 +/- 31 nmol/ml of packed erythrocytes. that of erythrocytes from patients with aplastic anaemia was 480 +/- 35 nmo ... | 1987 | 3567088 |
| the end-systolic pressure-volume relationship in conscious dogs. | the end-systolic pressure-volume relationship (espvr) has been shown to be an afterload-insensitive descriptor of ventricular inotropic state in the isolated heart. the purpose of this study was to examine the effects of changes in afterload, heart rate, intravascular volume, autonomic tone, and inotropic state on the espvr in conscious dogs. in 30 dogs, left ventricular and pleural pressures were measured with micromanometers, and left ventricular volume was assessed with global ultrasonic crys ... | 1987 | 3568332 |
| flow-cytometric characterization of stimulation, free radical formation, peroxidase activity and phagocytosis of human granulocytes with 2,7-dichlorofluorescein (dcf). | a standardized four-step assay for the flow cytometric determination of the oxidative activity of human polymorphonuclear leukocytes (pmnl) from normal human individuals and from septic patients was developed, using 2,7-dichlorofluorescin-diacetate (dcfh-da) as indicator for the intracellular formation of h2o2 and free radicals. spontaneous h2o2 and free radical formation was measured by preincubation of buffy coat pmnls from fresh peripheral venous blood at 37 degrees c and ph 7.4 with 10 micro ... | 1987 | 3569301 |
| amino acid sequence of thermostable direct hemolysin produced by vibrio parahaemolyticus. | the complete amino acid sequence of the subunit of thermostable direct hemolysin, a dimeric protein composed of identical subunits isolated from vibrio parahaemolyticus, was determined by sequencing brcn-peptides, their tryptic peptides, and overlaps obtained by achromobacter protease i digestion. the subunit consists of 165 amino acid residues with the sole disulfide bond between cys 151 and cys 161. it is deduced that the biologically active hemolysin is formed by noncovalent association of su ... | 1987 | 3571196 |
| a signal sequence domain essential for processing, but not import, of mitochondrial pre-ornithine carbamyl transferase. | studies using deletion mutagenesis indicate that a processing recognition site lies proximal to the normal cleavage position between gln32 and ser33 of pre-ornithine carbamyl transferase (poct). poct cdna was manipulated to delete codons specifying amino acids 22-30 of the signal sequence. the mutant precursor, designated poct delta 22-30, was imported to the matrix compartment by purified mitochondria, but remained largely unprocessed; the low level of processing that was observed did not invol ... | 1987 | 3571328 |
| further improvement of high-speed nmr flow-velocity measurement using a differential phase-encoding technique. | an nmr imaging technique applicable to high-velocity flow imaging is described. by subtraction of two phase images obtained by varying the flow-encoding gradient, it is possible to extract much larger ranges of velocity components. this differential phase-encoding technique is tested by experiments in a phantom as well as a human volunteer. | 1987 | 3574062 |
| pulsatile release of antipyretic neuropeptide alpha-msh from septum of rabbit during fever. | the effects on fever of central administration of alpha-melanocyte-stimulating hormone (alpha-msh) and its antiserum, the specific increase in central concentration of alpha-msh during fever, and related results indicate that this neuropeptide is important to central nervous system (cns) regulation of the febrile response. the present experiments were designed to establish whether alpha-msh is released from septal tissue during fever and, if so, in what temporal course relative to the febrile re ... | 1987 | 3591986 |
| laser influence on the rate of degeneration of damaged nerves. | the sciatic nerves were sectioned and sutured back. the rate of degeneration at the point of suture was studied. laser treatment increased the rate of degeneration of the damaged fibers. | 1987 | 3592253 |
| na+/adenosine co-transport in vibrio parahaemolyticus. | adenosine transport in vibrio parahaemolyticus was studied. na+ greatly stimulated adenosine uptake. addition of adenosine to a cell suspension under anaerobic conditions elicited na+ uptake, and the na+ uptake was inhibited by monensin, an na+ ionophore. imposition of an electrochemical potential of na+ or a membrane potential in energy-depleted cells elicited adenosine uptake. therefore, adenosine transport in this organism was concluded to proceed by an na+/adenosine co-transport mechanism. t ... | 1987 | 3607042 |
| serological studies of british leptospiral isolates of the sejroe serogroup. iii. the distribution of leptospires of the sejroe serogroup in the british isles. | some 94 strains of leptospires belonging to the sejroe serogroup isolated in the british isles were identified to the serovar level using specific factor sera. seventy strains were identified as leptospira interrogans serovar hardjo, 66 from cattle, 2 from pigs and 1 each from a sheep foetus and a human. twenty-four strains were identified as l. interrogans serovar saxkoebing, most strains were isolated from either wood mice, bank or field voles but strains were also isolated from badgers, a fox ... | 1987 | 3609169 |
| expression of "retinal" contrast gain control by neurons of the cat's lateral geniculate nucleus. | this paper describes the temporal tuning of cells in the lateral geniculate nucleus of the cat (27 x cells, 51 y cells) and how this changes with stimulus contrast. drifting sinusoidal gratings of optimal spatial frequency were presented at 7 temporal frequencies (0.5, 1, 2, 4, 8, 16 and 32 hz) and 4 contrasts (10, 20, 40, 80%). for some cells response growth at all temporal frequencies was proportional to changes in contrast. thus, their temporal tuning functions, on log-log axes, were displace ... | 1987 | 3609203 |
| extent of fiber regeneration after peripheral nerve repair: silicone splint vs. suture, gap repair vs. graft. | the degree of regeneration in surgically repaired sciatic nerves in rats was measured using a simple new electrophysiologic method: comparison of the size of nerve responses evoked by stimulation distal and proximal to the anastomosis. five different repair procedures were evaluated. after simple end-to-end suture anastomosis, about 40% of the severed parent fibers regenerated past the suture line. the result was substantially improved when the anastomosis was covered with a newly designed thinw ... | 1987 | 3609219 |
| the complete amino acid sequence of ribosomal protein s12 from bacillus stearothermophilus. | the amino acid sequence of ribosomal protein s12 from bacillus stearothermophilus has been completely determined. the sequence data were mainly obtained by manual sequencing of peptides derived from digestion with trypsin, staphylococcus aureas protease and pepsin. a few overlaps of tryptic peptides were established by dna sequence analysis of a chromosomal fragment containing the rpsl gene coding for ribosomal protein s12. the protein contains 138 amino acid residues and has an mr of 15,208. co ... | 1987 | 3542563 |
| trimethoprim used for selective decontamination of the digestive tract in rats: possible route of excretion. | selective elimination of enterobacteriaceae species from the digestive tract of rats has been accomplished by oral treatment with trimethoprim (235 mg/kg body weight/day) within 6 days. in the present study it was investigated whether this elimination was mainly due to antimicrobial activity of trimethoprim excreted with the gastrointestinal mucus or mainly by non-absorbed trimethoprim in the lumen contents. by means of whole gut irrigation (wgi) the lumen contents were washed out, followed by m ... | 1987 | 3497436 |
| the effects of backward pairings on the conditioning of the albino rabbit's jaw movement and nictitating membrane responses. | 1987 | 3502901 | |
| murine "housekeeping" enzyme (genetic locus: idh-1) is regulated in an allele-specific manner. | the murine "housekeeping" enzyme, cytosolic nadp-isocitrate dehydrogenase (e.c.1.1.1.42) (genetic locus: idh-1), exhibited a complex pattern of allele-specific expression. protein electrophoresis on cellulose-acetate gels and determination of relative enzymatic activity by means of densitometry revealed that in heart tissue (but not liver tissue) of certain hybrid crosses the aa-homodimer was underrepresented relative to total enzymatic activity, and the degree of underrepresentation changed dur ... | 1987 | 3502970 |
| a quantitative morphological examination of bovine vulval skin glands. | in adult cows the volumes of sweat and sebaceous glands per unit skin surface area (ssa) in the vulva were greater (p less than 0.01) than in the neck, lip or eyelids. within this perineal region skin gland volumes were greater dorsally than ventrally (p less than 0.01). adult cows had much greater (p less than 0.01) volumes of perineal skin glands per unit ssa than 8 weeks or 6 weeks old heifer calves or steers. there was little or no difference between animal categories in neck skin gland dime ... | 1987 | 3503046 |
| report of the committee on the genetic constitution of chromosomes 10, 11, and 12. | 1987 | 3507274 | |
| plasmid patterns, serotyping and kanagawa phenomenon of vibrio parahaemolyticus isolates from man, water and fish in calcutta, india. | 1987 | 3507428 | |
| enzyme-linked immunosorbent assay to detect shiga toxin of shigella dysenteriae and related toxins. | a sandwich enzyme-linked immunosorbent assay (elisa) was developed for detection of shiga toxin. four species of shigella, escherichia coli, and vibro parahaemolyticus were tested for production of shiga or shiga-like toxin by elisa and vero cell bioassay. in the elisa, most strains of s. dysenteriae and some strains of e. coli isolated from traveler's diarrhea were positive. these elisa-positive strains were positive by vero cell bioassay without exception. some e. coli strains and most v. para ... | 1987 | 3539985 |
| immunohistochemical demonstration of pyloric gland-type cells with low-pepsinogen isozyme 1 in preneoplastic and neoplastic tissues of rat stomachs treated with n-methyl-n'-nitro-n-nitrosoguanidine. | the appearance of pyloric gland-type cells with a low pepsinogen isozyme 1 (pg 1) content in the stomach mucosa of f344/du rats during stomach carcinogenesis was examined by a combination of paradoxical concanavalin a (con a) staining and immunohistochemical staining for pg 1. male f344 rats were given drinking water containing 100 micrograms n-methyl-n'-nitro-n-nitrosoguanidine [(mnng) cas: 70-25-7]/ml for 30 weeks and then normal tap water and were killed in week 10, 20, 30, 40, 50, or 70. unt ... | 1987 | 3470552 |
| reflections on infections by the sea. or another form of seasickness. | 1987 | 3473305 | |
| actions of prostaglandins e2 and f2 alpha on release of 14c-labelled mucins from rat submandibular salivary acini in vitro. | mucin secretion was studied in vitro using well-characterized preparations of isolated acini. pge2 significantly (p less than 0.05) increased release of [14c]-glucosamine labelled mucins at the highest concentration tested (10(-5) m), but was ineffective at lower doses (10(-9)-10(-6) m). pgf2 alpha had no effect on mucin secretion over this concentration range. pge2 (10(-9)-10(-5) m) did not modify isoproterenol stimulated mucin secretion. the cyclooxygenase inhibitor indomethacin (10(-7)-10(-5) ... | 1987 | 3482153 |
| surveillance of food poisoning and other food-borne diseases in singapore. | the more important food-borne diseases in singapore are food poisoning, enteric fever, cholera and hepatitis a. bacterial food poisoning caused by staphylococcus aureus, salmonella and vibrio parahaemolyticus is prevalent, but chemical poisoning due to ingestion of toxic plants and animals is uncommon. enteric fevers have been brought under control. an increasing proportion of the cases reported in recent years were imported from southeast asia and the indian subcontinent. cholera continues to b ... | 1987 | 3446000 |
| observation on a 65-kilodalton protein isolated from kanagawa positive strains of vibrio parahaemolyticus. | crude hemolysin from four kp+ strains of vibrio parahaemolyticus belonging to serotype 02:k3 exhibited a major protein band (molecular weight, 65 kilodaltons (kda] in addition to a previously known thermostable direct hemolysin band (molecular weight, 21 kda) in sds - polyacrylamide slab gel electrophoresis. these strains showed maximum virulence leading to 100% mouse lethality within 2-6 h. it is hypothesized that this 65-kda protein may play a vital role in the pathogenesis of the disease caus ... | 1987 | 3446347 |
| screening of vibrio parahaemolyticus isolates for plasmids. | 1987 | 3450594 | |
| hemolytic activity of and lethal toxin production by environmental strains of vibrio parahaemolyticus. | repeated subculturing of kanagawa-negative strains of vibrio parahaemolyticus on wagatsuma agar induced the production of a hemolysin which was not the thermostable direct hemolysin. crude hemolysin exhibited a 30 to 40% lethal toxicity in mice after intraperitoneal injection. a 21-kilodalton protein band was observed with all the environmental isolates in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. results suggested that a certain percentage of environmental strains of v. parahae ... | 1987 | 3426228 |
| specificity of a mycobacterium kansasii phenolic glycolipid (mycoside a) immunoserum. | the specificity of mycobacterium kansasii anti-mycoside a antiserum prepared in rabbits injected with purified samples of the phenolic glycolipid was evaluated by an enzyme-linked immunosorbent assay. chloroform-methanol extracts from representative strains of 23 mycobacterial species and 50 strains of m. kansasii showed that all strains of m. kansasii and the representative strain of m. gastri formed the antigen, whereas none of the remaining species (including m. leprae) formed it. consequentl ... | 1987 | 3429620 |