Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| nucleotide sequence and transcriptional analysis of the aercaera region of aeromonas sobria encoding aerolysin and its regulatory region. | the nucleotide sequence of a 2510 base pair chromosomal fragment containing the aerolysin gene aera, and its regulatory region aerc, from a clinical isolate of aeromonas sobria was determined. the aerolysin gene coded for a 54.5 kd polypeptide and had a g + c content of 59%, indicating that it is endogenous to the genus aeromonas. in contrast, the aerc region was characterized by its high a + t content (61%) and the presence of a core motif, aataaaa, repeated eight times within 300 base pairs. a ... | 1988 | 2459581 |
| molecular cloning, characterization, and nucleotide sequence of an extracellular amylase gene from aeromonas hydrophila. | the structural gene for excreted amylase from aeromonas hydrophila jmp636 has been cloned within a 2.1-kilobase smai fragment of dna. the amylase gene is transcribed from its own promoter in escherichia coli, producing a gene product of mr 49,000. the amylase gene product is secreted to the periplasm of e. coli; however, it is not excreted. nucleotide sequencing revealed an open reading frame of 1,392 base pairs corresponding to a protein of 464 amino acid residues. a potential signal peptide of ... | 1988 | 2449422 |
| comparison of four hemolysin-producing organisms (escherichia coli, serratia marcescens, aeromonas hydrophila, and listeria monocytogenes) for release of inflammatory mediators from various cells. | we investigated the role of various hemolysin-producing strains (escherichia coli, serratia marcescens, aeromonas hydrophila, and listeria monocytogenes) in induction of inflammatory mediators, e.g., histamine release from rat mast cells as well as the chemiluminescence response and the release of lipoxygenase transformation products from human polymorphonuclear neutrophils. our data show that the hemolysin-positive bacteria as well as the hemolysin-positive culture supernatants were active in i ... | 1988 | 2451679 |
| shigella flexneri invasion plasmid antigens b and c: epitope location and characterization with monoclonal antibodies. | invasion plasmid antigens b (ipab) and c (ipac) are associated with the ability of shigellae to invade cultured mammalian cells. monoclonal antibodies against ipab and ipac polypeptides were produced and used in a whole-cell enzyme-linked immunosorbent assay to show that both ipab and ipac polypeptides were exposed on the surface of virulent shigellae. moreover, these surface epitopes were shown to be highly conserved among different serotypes of shigella spp. and enteroinvasive escherichia coli ... | 1988 | 2459066 |
| virulence factors from aeromonas species. | isolates (116) of aeromonas were obtained from various sources and subjected to tests to establish their virulence factors. a high number of the isolates (69.8%) were found to be enterotoxigenic. the isolates from snails had more enterotoxigenic strains (73.3%), while those from cattle faeces had the lowest (33.3%). haemolysin production was found to be high (60.3%) amongst the isolates, and human isolates gave the highest number of haemolysin producing strains (70.6%), while the least number (3 ... | 1988 | 2468071 |
| s-layer of high-virulence aeromonas hydrophila. | 1988 | 2479668 | |
| serogrouping of the aeromonas hydrophila group. | 1988 | 2479669 | |
| gastro-enteritis in australian children: studies on the aetiology of acute diarrhoea. | a prospective, year-long study of the microbiology of childhood gastro-enteritis in perth showed that (i) aeromonas-associated diarrhoea was the commonest recognized bacterial cause for acute diarrhoea, especially in summer, (ii) campylobacter infections were the next most common bacterial cause, (iii) rotavirus and parasites were not significant causes of sporadic acute childhood diarrhoea, and (iv) enteric bacterial pathogens and intestinal parasites were often isolated from aboriginal childre ... | 1988 | 2456732 |
| molecular cloning of a phospholipid-cholesterol acyltransferase from aeromonas hydrophila. sequence homologies with lecithin-cholesterol acyltransferase and other lipases. | we have determined the nucleotide sequence of a gene encoding aeromonas hydrophila phospholipid-cholesterol acyltransferase, an enzyme which shares many properties with mammalian lecithin:cholesterol acyltransferase. the derived amino acid sequence of the protein contains two regions which are homologous to the proposed active sites and binding sites of the plasma acyltransferase and to similar sequences in other interfacially acting lipolytic enzymes. the amino terminus is preceded by a typical ... | 1988 | 3280033 |
| aeromonas eucrenophila species nova aeromonas caviae a later and illegitimate synonym of aeromonas punctata. | aeromonas caviae is a later and illegitimate synonym of aeromonas punctata. in order to secure continuity in literature, it is proposed to use the trivial term "caviae type" in connection with the species name a. punctata temporarily especially for strains isolated in connection to diarrhoea in children. aeromonas eucrenophila sp. nov. is described, type strain in ncmb 74. the new aerogenic psychrotrophic aeromonas species is separated genotypically from aeromonas hydrophila and aeromonas puncta ... | 1988 | 3394446 |
| seasonal distribution of aeromonas hydrophila in river water and isolation from river fish. | the seasonal distribution of aeromonas hydrophila in water and recovery rate from live river fish was investigated. the highest isolation rates of a. hydrophila occurred in water during the late winter followed by a progressive decline in density during the summer and monsoon seasons. the organism was recovered from fish throughout the period from which it was concluded that they form a reservoir which is unrelated to their density in water. the enterotoxigenicity of some environmental strains w ... | 1988 | 3220801 |
| aeromonas infection following a snake bite: a case report. | a twelve year-old thai boy with fulminating aeromonas hydrophila infection of the right arm following a snake bite was reported. he recovered uneventfully after appropriate antimicrobial therapy and surgical intervention. | 1988 | 3227407 |
| correlation of the suicide phenomenon in aeromonas species with virulence and enteropathogenicity. | certain strains of mesophilic aeromonads (aeromonas hydrophila, a. sorbria, and a. caviae), when grown in broth containing 0.5% glucose, undergo growth inhibition concomitant with acetate accumulation. because these strains are nonviable after 24 h, this phenomenon is termed suicide. we investigated suicidal strains of aeromonas species as a means of understanding animal virulence and enteropathogenicity. to assess virulence, batches of five white mice were inoculated intraperitoneally with 10(7 ... | 1988 | 3230136 |
| preparation of hemolysin from aeromonas. | 1988 | 3231101 | |
| immunoglobulin binding by the regular surface array of aeromonas salmonicida. | the cell surface of aeromonas salmonicida is covered by a regular surface array composed of a single species of protein, the a-protein (phipps, b. m., trust, t. j., ishiguro, e. e., and kay, w. w. (1983) biochemistry 22, 2934-2939). the array, known as the a-layer, is the key virulence factor for this organism. cells containing the a-layer specifically bound rabbit igg and human igm with high affinity (kd = 1.0 x 10(-6) m and 3.3 x 10(-6) m, respectively), but neither isogenic a-protein-deficien ... | 1988 | 2967820 |
| virulence properties and enterotoxin production of aeromonas strains isolated from fish. | the biological activities in vivo and in vitro of 59 motile aeromonas spp. isolated from fish and water tanks were simultaneously analyzed in poikilothermic and homoiothermic systems. a total of 64.3% of the isolates tested were pathogenic for fish, and 62% of aeromonas hydrophila and a. sobria isolates either virulent or nonvirulent for fish were enterotoxigenic. although the majority of the strains were proteolytic and amylolytic and produced dnase, other activities, such as elastase and staph ... | 1988 | 2972627 |
| an unusual case of pyogenic meningitis due to aeromonas sobria. | 1988 | 3382842 | |
| production of monoclonal antibody against aeromonas hydrophila haemolysin. | two hybridoma cell lines that produce monoclonal antibodies against aeromonas hydrophila haemolysin were established by fusion of myeloma and spleen cells obtained from a mouse immunised with haemolysin detoxified with tetranitromethane. enzyme-linked immunosorbent assay (elisa) showed that the two purified monoclonal antibodies, b7 and b11, recognised the same epitope on the haemolysin molecule. antibody b7 neutralised the haemolytic and enterotoxic activities of the haemolysin. it is concluded ... | 1988 | 3346901 |
| aeromonas hydrophila cellulitis. a case report. | 1988 | 3385616 | |
| aeromonas-associated gastroenteritis in children. | during a 20-month period 55 strains of aeromonas species were isolated from 53 children with diarrhea. the isolation rate of 2.5% for aeromonas compared with the rates of 4.5% for shigella, 3.3% for salmonella, 2.7% for campylobacter and 0.05% for yersinia. in 45 children aeromonas was the sole bacterial enteropathogen identified. aeromonas was also isolated from 2 (0.5%) of 380 asymptomatic children. despite its known lack of identifiable virulence properties, aeromonas caviae was the most prev ... | 1988 | 3340460 |
| aeromonas hydrophila wound infection in a patient with cyclic neutropenia following a piranha bite. | 1988 | 3340462 | |
| effect of incubation temperature on growth and soluble protein profiles of motile aeromonas strains. | motile aeromonas spp. are said to have an optimal growth temperature of 28 degrees c. we performed growth studies on 24 isolates at 29 and 37 degrees c and found that the optimal growth temperature was not necessarily 28 degrees c. we also detected temperature-dependent differences in soluble protein production in some of these strains. | 1988 | 3343335 |
| [prevalence of aeromonas spp. pediatric gastroenteritis]. | a selective medium and biochemical tests were used to search for aeromonas spp. in the stools of 536 children, more than 90% of whom had "gastroenteritis", seen at sainte-justine hospital, montreal, in june or july 1986. the organism was found in seven children, six of whom had bloody diarrhea. one of the six, a 6 1/2-year-old boy, required intravenous alimentation and later manifested ulcerative colitis; the other five recovered. the remaining child, a 14-year-old boy, had synovitis of the knee ... | 1988 | 3355951 |
| structure of an s layer on a pathogenic strain of aeromonas hydrophila. | negative staining revealed a tetragonal surface array (s layer) on all the members of a serogroup of aeromonas hydrophila which possess high virulence for fish. the s layers were similar on all the strains examined, with unit cell dimensions of approximately 12 nm. a single representative strain, strain tf7, was selected for further analysis. freeze-cleaved and etched preparations and sections for electron microscopy showed that the s layer was the outermost component of the cell envelope. this ... | 1988 | 3372478 |
| isolation and biochemical characterization of the s-layer protein from a pathogenic aeromonas hydrophila strain. | the regular surface protein array (s layer) present on aeromonas hydrophila tf7 is composed of a single species of protein of apparent molecular weight 52,000. this protein was extracted from whole cells by treatment with 0.2 m glycine hydrochloride (ph 3.0). the protein was purified to homogeneity by ion-exchange chromatography and reverse-phase high-performance liquid chromatography. amino acid composition analysis showed that the protein contained 520 residues per molecule, 41% of which were ... | 1988 | 3372479 |
| isolation of aeromonas salmonicida from masu salmon in the republic of korea. | the isolation and identification of the gram-negative fish pathogen, aeromonas salmonicida salmonicida, from masu salmon (oncorhynchus masou) at the yang yang salmon hatchery in the republic of korea is described. the bacterium possessed certain characteristics in common with isolates from fish in japan. it is an important cause of mortality among cultured salmonids in the republic of korea. this is the first report of this fish pathogen from the asian mainland. | 1988 | 3373645 |
| the phylogenetic origin of the bifunctional tyrosine-pathway protein in the enteric lineage of bacteria. | because bifunctional enzymes are distinctive and highly conserved products of relatively infrequent gene-fusion events, they are particularly useful markers to identify clusters of organisms at different hierarchical levels of a phylogenetic tree. within the subdivision of gram-negative bacteria known as superfamily b, there are two distinctive types of tyrosine-pathway dehydrogenases: (1) a broad-specificity dehydrogenase (recently termed cyclohexadienyl dehydrogenase [cdh]) that can utilize ei ... | 1988 | 3386529 |
| screening of bacteria with antiviral activity from fresh water salmonid hatcheries. | bacteria isolated from two salmonid hatcheries were screened for antiviral activity against infectious hematopoietic necrosis virus (ihnv) to ascertain the presence of bacteria with anti-ihnv activity in the aquatic environment. out of 710 bacterial isolates from the water and sediment samples, 190 strains showed anti-ihnv activities of more than 50% plaque reduction. these antiviral activities were detected predominantly in pseudomonas, aeromonas/vibrio, and coryneforms. in one hatchery, the ba ... | 1988 | 3374405 |
| [analysis of degradative plasmids by pyrolysis gas liquid chromatography]. | 1988 | 3388828 | |
| oxolinic acid in the trout: bioavailability and tissue residues. | a study was performed on the serum bioavailability and tissue elimination of oxolinic acid in the trout. the compound was added to the diet and administered at a dosage-level of 12 mg/kg/day for 7 consecutive days. the study utilized an analytical technique, high performance liquid chromatography, which has been described in detail here. the results obtained demonstrate that serum concentrations higher than the mic for the control of the target pathogens (aeromonas and yersinia) can by sustained ... | 1988 | 3400972 |
| [frequency of aeromonas species in the stools of healthy and diarrheic patients]. | during two test periods (summer 1985 and january-august 1986) a total of 7713 stool specimens were examined for the occurrence of aeromonas spp. the isolation rate of the asymptomatic group was about 0.3%, the same rate was found in adults with diarrhea. in the stool specimens of 364 children 8 aeromonas spp. could be found. a. caviae was isolated in most cases of diarrhea. data obtained suggest that it seems to be useful to screen all diarrhoic stools of children for the occurrence of aeromonas ... | 1988 | 3402999 |
| surface properties of autoagglutinating mesophilic aeromonads. | the surface characteristics of 24 autoagglutinating (aa+) mesophilic aeromonads were investigated. one group of 16 was found to be highly related serologically by their reactive pattern against o antisera generated against three reference strains. subsequent characterization of 11 of these isolates (group 1) indicated that they had the following properties in common: precipitation after boiling (pab+), membership of serogroup o:11 (typing scheme of sakazaki and shimada), resistance to lysis by b ... | 1988 | 3417353 |
| [antibiotic resistance of several gram-negative bacteria from clinical and dissection material from the institute for poultry diseases of ludwig maximilian university, munich]. | a survey is given on antibiograms carried out with important bacterial strains isolated from birds during 1987. as compared with the results from earlier years no significant changes could be shown, the sensitivity ranging from 42.4% to 95.9%. the genera bordetella and aeromonas were included for the first time. | 1988 | 3420603 |
| the effects of gamma irradiation on the lymphoid organs of rainbow trout and subsequent susceptibility to fish pathogens. | fish were irradiated with 60co gamma rays at doses ranging from 10 to 50 gy. lethal doses were determined in fishes of different ages. for a given dose, fry and fingerlings were more susceptible than subadults. whatever the irradiation dose was, the fish displayed a sharp decrease in blood leucocyte count. at the lowest doses, this acute leucopaenia was reversible. the cellular damage in the lymphoid organs was particularly obvious in the thymus. the depletion of lymphoid cells from immunocompet ... | 1988 | 3388762 |
| antibiotic resistance patterns of aeromonas spp. isolated from environmental and clinical sources. | microbiologists from the international centre for diarrhoeal disease research, bangladesh (icddr,b) collected water, sediment, plant, and plankton samples from a lake and a river in dhaka, bangladesh to isolate aeromonas spp. they also took clinical isolates from patients at the dhaka hospital of icddr,b. the microbiologists identified at least 154 isolates of aeromonas and analyzed them for antibiotic susceptibility using the standard agar disc diffusion method. 92.8% of the environmental is ... | 1988 | 12342624 |
| [aeromonas spp. and plesiomonas shigelloides in bivalves, mud, and water from the gulf of nicoya, costa rica]. | bivalves, mud, and surface water were collected at three different sites of the gulf of nicoya, costa rica, in search of aeromonas spp. and plesiomonas shigelloides. for their isolation, these bacteria were enriched in alkaline peptone water and streaked on macconkey agar and on brilliant green bile inositol agar. this was followed by the biochemical tests necessary for their identification. thirty-five strains of a. hydrophila, 58 of a. caviae, 43 of a. sobria, and 7 of p. shigelloides were iso ... | 1989 | 9700365 |
| bacterial colonization of cod (gadus morhua l.) and halibut (hippoglossus hippoglossus) eggs in marine aquaculture. | aquaculture has brought about increased interest in mass production of marine fish larvae. problems such as poor egg quality and mass mortality of fish larvae have been prevalent. the intensive incubation techniques that often result in bacterial overgrowth on fish eggs could affect the commensal relationship between the indigenous microflora and opportunistic pathogens and subsequently hamper egg development, hatching, larval health, and ongrowth. little information about the adherent microflor ... | 1989 | 16347937 |
| solubilization of fish proteins using immobilized microbial cells. | cells of bacillus megaterium, aeromonas hydrophila, and pseudomonas marinoglutinosa were immobilized in calcium alginate. the immobilized cells secreted protease when held in fish meat suspension in water. the enzyme synthesis by the entrapped cells was supported by small amounts of soluble nutrients present in the meat. the secreted protease solubilized the fish meat, solubilization being optimum at ph range of 7.5 to 9.5 and at 50 degrees c. under these conditions immobilized b. megaterium was ... | 1989 | 18588026 |
| occurrence of aeromonas hydrophila in wild birds. | aeromonas hydrophila was isolated from 135 of a total of 1226 wild birds. it was the only bacterial species in eight birds, while other bacteria, mainly enterobacteriaceae, staphylococci and/or streptococci were identified in all other birds. the rate of isolation from aquatic birds (18.5%) was higher (p<0.001) than from birds of terrestrial habitats (3.4%). infection may also depend on dietary habits: 7.0% of the granivorous and herbivorous species, 8.4% of the omnivores and 12.0% of carnivorou ... | 1989 | 18679900 |
| [pleural empyema caused by aeromonas hydrophila]. | 1989 | 2490662 | |
| [endocarditis caused by aeromonas hydrophila]. | 1989 | 2490663 | |
| cutaneous manifestations of bacterial sepsis. | in short, bacterial sepsis is associated with a number of peripheral manifestations involving the skin and soft tissues. the pathogenesis of the lesions observed is not fully understood and is almost certainly multifactorial. in ecthyma gangrenosum, the presence of large numbers of gram-negative bacilli in the walls of small blood vessels without a substantial inflammatory response suggests that either the bacteria themselves or bacterial products are responsible for tissue damage. endotoxin pro ... | 1989 | 2523895 |
| [intestinal infections caused by aeromonas]. | we have identified 38 instances of intestinal infection by aeromonas and 4 by plesiomonas in 16,184 coprocultures carried out during a 4 year period in the hcu of zaragoza. the low prevalence can be related with the fact that specific media for the recovery of these organisms were not used. the species aeromonas sobria (47.36%) and a. caviae (39.47%) were more common than a. hydrophila (13.15%). aeromonas were isolated from patients with and without intestinal disease. the occurrence of gastroin ... | 1989 | 2490673 |
| [application of the heiberg biotypes in the diagnosis of species in the mesophilic aeromonas group]. | 1989 | 2490869 | |
| [bacterial etiologies of acute infantile diarrhea in dakar]. | systematic research into all the bacterial etiological agents responsible for acute diarrhoea was undertaken on 276 diarrhoea stools collected from children aged 0-5 years. particular attention was given to finding bacterial agents usually overlooked during routine checks: campylobacter, vibrionaceae and yersinia enterocolitica. | 1989 | 2491376 |
| genetics and molecular biology of siderophore-mediated iron transport in bacteria. | the possession of specialized iron transport systems may be crucial for bacteria to override the iron limitation imposed by the host or the environment. one of the most commonly found strategies evolved by microorganisms is the production of siderophores, low-molecular-weight iron chelators that have very high constants of association for their complexes with iron. thus, siderophores act as extracellular solubilizing agents for iron from minerals or organic compounds, such as transferrin and lac ... | 1989 | 2531838 |
| hygienic status of food handlers. | the study demonstrated bacterial species on hands and nails of food-handlers before and after hand-washing. those were staphylococcus spp., streptococcus spp., micrococcus spp., bacillus spp., diphtheroid, aeromonas hydrophila, klebsiella pneumoniae, acinetobacter, enterobacter cloacae, escherichia coli, pseudomonas spp., proteus mirabilis, serratia spp., citrobacter freundii. before hand washing, each food-handler harboured one to eight bacterial species. after hand-washing (eight with water fr ... | 1989 | 2584903 |
| physiological control of amonabactin biosynthesis in aeromonas hydrophila. | amonabactin is a siderophore from aeromonas hydrophila which is produced in two biologically active forms composed of the phenolate 2,3-dihydroxybenzoic acid (dhb), lysine, glycine, and either trytophan (amonabactin t) or phenylalanine (amonabactin p). amonabactin biosynthetic mutants (generated by chemical mutagenesis) that either produced no amonabactin or overproduced the siderophore were isolated and identified on chrome azurol s siderophore detection agar. amonabactin-negative mutants were ... | 1989 | 2535179 |
| [infectious gastroenteritis in the immunocompetent child. significance of cryptosporidium spp. and aeromonas ssp]. | over a one-year period we have examined 390 stool specimens from 210 children hospitalized for infectious gastroenteritis or in whom infectious gastroenteritis occurred during hospitalization. the most prevalent pathogens were rotaviruses (22.4%), followed by enteric salmonella (8.1%), campylobacter jejuni/coli (2.4%), cryptosporidium spp. (2.4%) and aeromonas spp. (1.9%). both cryptosporidium spp. and aeromonas spp. are important pathogens of infectious gastroenteritis in immunocompetent childr ... | 1989 | 2540525 |
| isolation and characterization of monoclonal antibodies against alkaline phosphatase of pseudomonas aeruginosa. | monoclonal antibodies against the alkaline phosphatase of pseudomonas aeruginosa were produced from spleen cells of balb/c mice primed with purified alkaline phosphatase of p. aeruginosa atcc 10145 and sp20/ag-14 myeloma cells. the eight stable clones established produced antibodies that reacted by enzyme-linked immunosorbent and indirect immunofluorescence assays with all bacterial strains of p. aeruginosa, including the 17 serotypes and two nontypable strains. three of the clones cross-reacted ... | 1989 | 2501343 |
| aeromonas: the forgotten pathogen. | 1989 | 2504945 | |
| lomefloxacin (sc 47111 or ny-198), a new difluorinated quinolone: comparison of the in vitro activity with other broad spectrum antimicrobials against enterobacteriaceae, acinetobacter spp, aeromonas spp, and pseudomonas aeruginosa. | using a broth microdilution method, we compared the in vitro activity of lomefloxacin to other broad spectrum antimicrobials against clinical strains of enterobacteriaceae, acinetobacter spp, aeromonas spp, and p. aeruginosa. against the enterobacteriaceae and a. hydrophila, lomefloxacin showed excellent activity with mic50 values ranging from 0.12-0.5 micrograms/ml and mic90 values ranging from 0.12-1.0 micrograms/ml. by comparison, lomefloxacin had superior activity to nalidixic acid and diflo ... | 1989 | 2507219 |
| complexing of copper in drinking water samples to enhance recovery of aeromonas and other bacteria. | the presence of copper in drinking water samples at concentrations as low as 10 micrograms/l resulted in a rapid die-off of aeromonads. coliform bacteria, heterotrophic plate count bacteria and faecal streptococci were also sensitive to copper but to a lesser degree than aeromonads. the effect was particularly noticeable in soft water (less than 3 meq/l ca + mg) and at ph-values below 8.0. the toxic effect of copper concentrations up to 500 micrograms/l could be neutralized for a period of up to ... | 1989 | 2512276 |
| protection of rainbow trout against vibriosis and furunculosis by the use of attenuated strains of vibrio anguillarum. | the fish pathogen vibrio anguillarum causes a lethal infection in rainbow trout (salmo gairdneri). three different avirulent mutants, constructed by transposon insertion mutagenesis (van20 and van70) or as antibiotic-resistant mutants (van1000), were isolated by screening 200 individual isolated mutants for avirulence. when used as live vaccines, all three avirulent mutants were able to induce protective immunity against the homologous as well as a heterologous strain of v. anguillarum. when van ... | 1989 | 2548446 |
| influence of modified-atmosphere storage on the growth of uninjured and heat-injured aeromonas hydrophila. | the growth of uninjured and heat-injured aeromonas hydrophila incubated at 5 degrees c (22 days) and 30 degrees c (31 h) under air, n2, and co2 was investigated. at 30 degrees c, the growth patterns of cells on brain heart infusion agar incubated under air and n2 were similar, although slight differences in the lengths of the lag phases and the final populations were detected. the lag phases of cells incubated under air and n2 were substantially longer at 5 degrees c than at 30 degrees c. the po ... | 1989 | 2516434 |
| purification of igg from serum with caprylic acid and ammonium sulphate precipitation is not superior to ammonium sulphate precipitation alone. | immunoglobulin g (igg) from bovine serum raised against aeromonas salmonicida was purified by ammonium sulphate precipitation (asp) or caprylic acid treatment followed by ammonium sulphate precipitation (caas). purity of igg samples prepared by both methods were examined by high performance gel permeation chromatography, electrophoresis and antibody activity assay. results suggest that igg prepared by asp is better than that obtained by caas method in terms of the yield of the igg monomers and t ... | 1989 | 2612135 |
| evolutionary relationships among bacterial carbamoyltransferases. | an immunological approach was used for the study of ornithine carbamoyltransferase (otcase) evolution in bacteria. antisera were prepared against the anabolic and catabolic otcases of pseudomonas aeruginosa and aeromonas formicans as well as against otcase and putrescine carbamoyltransferases from streptococcus faecalis; these antisera were then tested against the unpurified otcases, either anabolic or catabolic, of 34 bacterial strains. extensive cross-reactions were observed between the antise ... | 1989 | 2516870 |
| purification and characterization of aeromonas hydrophila beta-hemolysin. | aeromonas hydrophila hemolysin was excreted in our culture conditions during the stationary growth phase. the toxin was purified to homogeneity by a three-step method: ultrafiltration, acid precipitation in the presence of rna and anion exchange chromatography with fplc apparatus. beta-hemolysin is a protein not associated with lipids, carbohydrates or nucleic acids whose subunit mol. wt is 51,000. the mol. wt determined by polyacrylamide gel electrophoresis suggests that the molecule is in a tr ... | 1989 | 2516922 |
| comparative in vitro activities of piperacillin-tazobactam and ticarcillin-clavulanate. | the in vitro activities of ticarcillin, piperacillin, clavulanic acid, tazobactam, ticarcillin-clavulanate, and piperacillin-tazobactam against 819 bacterial isolates were compared. the two beta-lactamase inhibitors, clavulanic acid and tazobactam, had little useful antibacterial activity but enhanced the activities of the penicillins against beta-lactamase-producing strains of haemophilus influenzae, branhamella catarrhalis, and methicillin-susceptible staphylococcus aureus; all strains were su ... | 1989 | 2552904 |
| enterotoxin induced diarrhoea--an update. | the pathogenic personality or the criteria required to be a successful pathogen, of enteric bacteria includes, among others, the ability to produce potent proteins which by different intracellular mechanisms elicit what we overtly see as diarrhoea. enteropathogens belonging to several genera like vibrio, escherichia, shigella, salmonella, campylobacter, aeromonas and yersinia include species capable of elaborating strikingly similar exotoxins which seem to share common mechanisms of action invol ... | 1989 | 2554547 |
| [bacterial isolation in infants hospitalized for acute diarrhea]. | bacteria were investigated in stools of 156 children under two years of age admitted to the pediatric wards of a general hospital at the western metropolitan area of santiago, chile, because of acute diarrhea with only one stool sample. a known pathogenic agent was isolated from 115 cases (73.7%), this being a bacteria in 87/115 (75.6%). most prevalent microorganisms were enteropathogenic escherichia coli (epec). (65.5%), specially serogroups 0111, 0119 and 055, enterotoxigenic escherichia coli ... | 1989 | 2520838 |
| recovery of viruses and bacteria in waters off bondi beach: a pilot study. | a pilot study was conducted between february and april, 1989, on the occurrence of sewage-derived viruses and bacteria in the beach and nearshore waters off bondi, sydney. enteroviruses were isolated from 41% of a total of 66 sewage, sea-water, grease and sediment samples. poliovirus vaccine strains accounted for 78% of the isolates. adenoviruses were isolated four times and coxsackievirus b was isolated twice in samples that were collected away from the bathing area. rotavirus and hepatitis a v ... | 1989 | 2556634 |
| aeromonas species in aquaria: a reservoir of gastrointestinal infections? | to assess the role of aquaria in the epidemiology of aeromonas associated gastroenteritis, the prevalence and antibiotic susceptibility of aeromonads in ornamental aquaria were determined and compared to that of isolates obtained from patients with gastroenteritis. thirty-eight (76%) of 50 aquaria, including those located in our hospital, yielded 61 isolates: 24 (39.3%) aeromonas sorbria, 18 (29.0%) a. hydrophila, 15 (24.6%) a. caviae, and four (6.5%) which could not be speciated. in comparison, ... | 1989 | 2567310 |
| amonabactin, a novel tryptophan- or phenylalanine-containing phenolate siderophore in aeromonas hydrophila. | aeromonas hydrophila 495a2 excreted two forms of amonabactin, a new phenolate siderophore composed of 2,3-dihydroxybenzoic acid, lysine, glycine, and either tryptophan (amonabactin t) or phenylalanine (amonabactin p). supplementing cultures with l-tryptophan (0.3 mm) caused exclusive synthesis of amonabactin t, whereas supplements of l-phenylalanine (0.3 to 30 mm) gave predominant production of amonabactin p. the two forms of amonabactin were separately purified by a combination of production an ... | 1989 | 2522922 |
| [the occurrence of escherichia coli, aeromonas hydrophila, plesiomonas shigelloides and clostridium perfringens in the intestinal flora of gray herons (ardea cinerea)]. | the flora of the large intestine of 92 grey herons was examined for the frequency of aerobic and microaerobic growing bacteria. clostridium perfringens, aeromonas hydrophila, plesiomonas shigelloides and e. coli were isolated from 55%, 48%, 14% and 35% of the birds, respectively. it could be demonstrated that the findings of these bacteria in the intestinal flora are depending on the age of the birds. the percentage of carriers of clostridium perfringens, aeromonas hydrophila and plesiomonas shi ... | 1989 | 2548355 |
| characterization of a pilus produced by aeromonas hydrophila. | a pilus produced by aeromonas hydrophila was purified and partially characterized. the pilin monomers had an apparent molecular weight of 17,000. agglutination studies indicated serological cross-reactivity in the pili of a. hydrophila strains. presence of pili did not correlate with hydrophobicity or haemagglutinating ability of the bacteria. | 1989 | 2569431 |
| motile aeromonas infection of striped (grey) mullet mugil cephalus. | infection of striped mullet (mugil cephalus) with aeromonas hydrophila results in an acute septicemic disease. the disease can be experimentally induced by intramuscular injection, skin or gill scarification or by the oral route using pellets purposely seeded with bacteria. the organism was isolated from the blood 1-2 days after infection and from all organs 24 hr or longer after infection. the disease is characterized by early inflammatory and proliferative changes and later necrotic changes. e ... | 1989 | 2575883 |
| antimicrobial susceptibility of sixty human fecal isolates of aeromonas species. | the mics of 21 antimicrobial agents were determined for 60 strains of aeromonas spp. isolated from human feces. all isolates tested were susceptible to aztreonam, tetracycline, imipenem, moxalactam, pipemidic acid, gentamicin, trimethoprim-sulfamethoxazole, pefloxacin and ciprofloxacin. resistance to erythromycin and streptomycin was observed in all 60 strains. aeromonas caviae was less susceptible to cefamandole, cefotaxime, norfloxacin, chloramphenicol, tetracycline, sulfamethoxazole and trime ... | 1989 | 2496995 |
| conjugation system of incc plasmid ra1, and the interaction of ra1 pili with specific rna phage c-1. | ra1 was the only incc plasmid that was slightly temperature-sensitive for replication and transfer. at 30 degrees c, ra1 determined constitutive synthesis of conjugative pili and yet was transfer-repressed. attachment of shaft-adsorbing rna phage c-1 virions prevented the probable retraction of pili under heat stimulus (55 degrees c). electron microscopy showed single adsorbed virions at pilus bases where they were thought to have stopped retraction. | 1989 | 2576151 |
| bombesin: an activator of specific aeromonas antibody secretion in rat intestine. | the effect of bombesin (bbs) in modulating the secretion of specific aeromonas antibodies in rat intestine was determined. rats were immunized with the culture supernatant of aeromonas hydrophila, isolate ssu. this culture supernatant contained a number of toxins that may be considered virulence factors. after 24 days of immunization, rats were anesthetized and a 10-cm intestinal segment was perfused with phosphate-buffered saline. the effluents were collected for measurement of iga and igg by t ... | 1989 | 2582984 |
| chronic colitis after aeromonas infection. | 1989 | 2583575 | |
| aeromonas hydrophila strains with moderate virulence. | a group of aeromonas hydrophila strains was obtained from very different origins which showed very similar characteristics (lipopolysaccharide, outer-membrane protein profile, toxin production, bacteriophage sensitivity and moderate virulence), besides the heterogenicity observed among the a. hydrophila strains. this group of a. hydrophila strains with moderate virulence can be easily recognized by their bacteriophage sensitivity pattern. | 1989 | 2593869 |
| in-vitro activity of lomefloxacin in comparison with pefloxacin and ofloxacin. | the in-vitro activity of lomefloxacin (sc 47111, ny-198) was investigated by the determination of mics in agar and in broth, of mbcs in broth, of killing curves and of the duration of the post-antibiotic effect. mics measured in broth and in agar were almost identical. lomefloxacin was two- to eight-fold less active against gram-positive bacteria than ofloxacin. its activity against staphylococcus aureus was independent of resistance to penicillin and oxacillin. the activity of lomefloxacin, ofl ... | 1989 | 2496071 |
| [infectious diarrhea in the adult]. | infectious diarrhoeas are usually divided into two types; toxinogenic and invasive. invasive diarrhoeas are copious and responsible for dehydration which is the principal clinical sign; mucosal lesions and bacteraemia are absent. the most typical of toxinogenic diarrhoeas is cholera, but enterotoxicogenic e. coli and aeromonas infections have similar clinical features. in invasive diarrhoeas the responsible microorganisms penetrate into the epithelial cells of the intestine, producing fever and ... | 1989 | 2602888 |
| mixed infections in childhood diarrhoea: results of a community study in kiambu district, kenya. | one thousand four hundred and twenty diarrhoea specimens from 846 children aged 0 to 60 months were collected and analysed for bacteria, parasites and rotavirus over a 16 month period, from june 1985 to september 1986 inclusive. the study was conducted in 4 villages situated in kiambu district, kenya. all the specimens were analysed for rotavirus and parasites, including cryptosporidium. the majority of the specimens were analysed for enteropathogenic escherichia coli (epec), shigella, salmonell ... | 1989 | 2606013 |
| oral cholera vaccines containing b-subunit-killed whole cells and killed whole cells only. ii. field evaluation of cross-protection against other members of the vibrionaceae family. | because of demonstrable cross-reactivity of cellular antigens contained in b subunit-killed whole-cell (bs-wc) and killed whole-cell-only (wc) oral cholera vaccines with antigens of various non-cholera species of the family vibrionaceae (ncv), the protection conferred by the vaccines against diarrhoea associated with ncv was evaluated during a randomized, double-blind field trial in bangladesh. children aged 2-15 years and women aged greater than 15 years (62,285 in number) received three doses ... | 1989 | 2665350 |
| some observations on the faecal carriage of mesophilic aeromonas species in cows and pigs. | replicate faecal samples from healthy individual pigs and cows were examined for the presence of aeromonas sp. over a 12-month period. aeromonads were found to be minor components of the faecal flora, only 8.8% of 520 samples from pigs and 4.6% of 481 samples from cows proving positive. isolation rates in both groups of animals were seasonal. a hydrophila (62% of the isolates) was the predominant species in cows, followed by a. caviae (32%) and a. sobria (15%). this pattern was also recorded in ... | 1989 | 2606161 |
| serogroups of aeromonas species from clinical and environmental sources in calcutta, india. | using a recently developed somatic o-antigen serogrouping scheme, 118 isolates of aeromonas spp. were serogrouped, 68 of which were isolated from diarrhoea patients admitted to the infectious diseases hospital and b.c. roy children's hospital, calcutta, from march 1987 to february 1988 and 50 randomly selected from an environmental study conducted during the same period. the isolates were typed into 23 different serogroups which represent 35.9% of the total 64 o-serogroups in the typing scheme. ... | 1989 | 2607101 |
| isolation of medically significant vibrio species from riverine sources in south east queensland. | vibrio and vibrio-like bacteria were isolated from water, sediments, plants and faeces from eight riverine sites in south east queensland, australia, using filtrations, enrichments and selective growth on thiosulphate-citrate-bile salts-sucrose (tcbs) and simidu agars. isolates (402 in toto) were classified by numerical analysis of phenotypic characteristics and comparison with fifteen reference cultures. of the isolates 41 (10.2%) were identified as vibrio cholerae, 33 (8.2%) as v. fluvialis an ... | 1989 | 2607994 |
| [are aeromonas species a cause of diarrheal diseases in yugoslavia?]. | isolation and identification of aeromonas spp. have been performed in 1,610 stools in the period from january 1987 to april 1988. of 1300 stool specimens taken from the patients with diarrheal syndrome (540 stools of children and 760 stools of adults) aeromonas spp. were isolated only in 4 patients (0.30%, p less than 0.001). positive stools were found in 3 adult patients and one child. in 310 examined stools of healthy adult persons aeromonas spp. were not isolated. a significantly higher incid ... | 1989 | 2609506 |
| incidence of aeromonads in samples from an abattoir processing lambs. | two hundred and thirty three samples of lamb carcasses, livers, kidneys and faeces, collected at a local abattoir, were examined to determine the incidence of motile aeromonas spp. wash water from the abattoir was also tested. direct plating on starch ampicillin agar and enrichment in alkaline peptone water were used. the incidence of aeromonads was low. they were detected only after enrichment in 5/47 faecal samples and 11/50 carcass samples. the 41 strains of aeromonas isolated were identified ... | 1989 | 2613594 |
| [studies on bacterial agents in acute diarrheal disease (1985-1987)]. | a three-year epidemiological study (from january 1985 to december 1987) was carried out on sporadic cases of acute diarrhea. a total of 2889 fecal specimens in cary-blair transport medium were examined for bacterial enteric pathogens, and 832 strains of fifteen species were isolated from 739 specimens, 73 patients having two or more pathogens. c. jejuni shared 51.7%, salmonella spp. 18.3%, v. parahaemolyticus 10.3%, and aeromonas spp. 15.7% of total fecal specimens. isolation rates of c. jejuni ... | 1989 | 2614097 |
| isolation of aeromonas spp. from water by using anaerobic incubation. | a membrane filtration method incorporating a combination of anaerobic and aerobic incubation has been developed for the enumeration of aeromonas spp. in drinking water. the use of anaerobic incubation improved the detection of aeromonas spp. by reducing the growth of nonaeromonads. the confirmation rate of presumptive aeromonas spp. identified on the initial isolation agar exceeded 92%. | 1989 | 2679380 |
| aeromonas endocarditis in a patient with chronic hepatitis-b infection. | aeromonas, a genus of gram-negative bacteria normally found in water and soil, is well established as a pathogen in the animal kingdom. often considered as a pathogen of low virulence, its role in human infections has recently been recognised. aeromonas infections in humans range from cellulitis to septicaemia. endocarditis is rare. we describe here a patient with a chronic liver disease with aeromonas bacteremia and endocarditis. | 1989 | 2617304 |
| [long-term cryopreservation of microbial strains relevant to food hygiene]. | experiments on long-time cryopreservation at -20 degrees c were conducted, using 42 microbial strains of relevance to food hygiene. blood slant agar was coated with 10% glycerol solution, so that all strains were in re-culturing conditions, following 12 months of storage. re-culturing of 18 in 20 of the microbial genera used in the experiment was possible after 24 months of storage, if strains had been kept only in 10% glycerol solution. the method proved suitable particularly for strain keeping ... | 1989 | 2619482 |
| bacterial and viral causes of acute diarrhoea in children in kuwait. | bacterial and viral causes of acute diarrhoea were studied prospectively for one year in 343 hospitalised young children in kuwait. in 288 (84%) patients, one or more pathogens were identified compared with 12 of 86 (13.9%) children admitted with diseases other than diarrhoea (p less than 0.01). forty-four (12.9%) of the patients were infected with two or more pathogens. viral agents detected in the stools were rotaviruses (40.2%), enteric adenoviruses (1.7%), and enteroviruses (1.5%). enterobac ... | 1989 | 2632640 |
| aeromonas spp. in foods: a significant cause of food poisoning? | from a total of 563 samples of various foodstuffs purchased from retail outlets in the reading area 287 were found to contain mesophilic aeromonas spp. the types of sample which were most frequently contaminated were poultry (79.3%) and offal (84.3%). of three media compared for their efficiency in recovering aeromonas spp. after enrichment in alkaline peptone water, difco aeromonas was the most efficient. cytotoxin was produced by approximately 50% of the a. hydrophila and a. sobria strains but ... | 1989 | 2641273 |
| toxin production by strains of aeromonas hydrophila grown in laboratory media and prawn purée. | toxin production by four strains of aeromonas hydrophila grown at 30 and 37 degrees c in two laboratory media and prawn purée was studied. three different cell lines were used to test for cytotoxic activity, haemolytic activity was tested against rabbit and guinea pig erythrocytes, proteolytic activity was assayed with azo-casein and enterotoxic activity using the suckling mouse assay. results showed reduced cytotoxic and haemolytic activities in prawn purée compared with the two media, but in m ... | 1989 | 2641490 |
| effect of temperature on hemolysin production in aeromonas spp. isolated from warm and cold environments. | the effect of growth temperature on the hemolysin production in 97 aeromonas isolates was studied. only 9% of the isolates obtained from low-temperature sources such as refrigerated foods, drinking water and trout aquaculture were able to produce high hemolysin titers (titer greater than 128) at 37 degrees c compared with 65% of the isolates from warm sources such as human clinical cases and warm water aquaculture (p less than 0.001). in contrast, 61% of the low-temperature isolates were produci ... | 1989 | 2641493 |
| enterotoxigenicity and drug sensitivity of aeromonas hydrophila isolated from well water in sweden: a case study. | a large number of aeromonas spp. have been found in drinking water from a drilled well in sweden. isolates identified as a. hydrophila were tested for production of enterotoxin, hemolysin, enzymes and for resistant patterns to different antibiotics. the enterotoxin-producing a. hydrophila could be responsible for the long-term diarrhoeal case of a 1 1/2 year old child who consumed the contaminated water. | 1989 | 2641888 |
| automated biochemical identification of bacterial fish pathogens using the abbott quantum ii. | the quantum ii, originally designed by abbott diagnostics for automated rapid identification of members of enterobacteriaceae, was adapted for the identification of bacterial fish pathogens. the instrument operates as a spectrophotometer at a wavelength of 492.600 nm. a sample cartridge containing 20 inoculated biochemical chambers is inserted in the path of the analyzing beam. reactions are converted into a 7-digit octal biocode, relayed via a sensor to the memory module, and compared to biocod ... | 1989 | 2644450 |
| aeromonas as a human pathogen. | although the first aeromonas strain was described by zimmermann as early as in 1890, it took 60 years until caselitz established human pathogenicity of strains then called "vibrio jamaicensis". since then, and especially in the last 10 years, there have been increasing numbers of reports on different infections caused by members of the genus aeromonas. these include sepsis; meningitis; cellulitis; necrotizing fasciitis; ecthyma gangrenosum; pneumonia; peritonitis; conjunctivitis; corneal ulcer; ... | 1989 | 2649316 |
| [significance of the occurrence of aeromonas of various biotypes in the third world]. | in investigations carried out in the refugee camps of the polisario (south algeria), in the neighbourhood of the inhabitants of the waste disposal sites of cairo and in the ayoun el atrous and nema (mauritania) regions also in the bida and minna hospitals. aeromonas hydrophila and aeromonas spec. were repeatedly found in the investigated material. therefore, it was of particular interest for us to investigate this type of organism. | 1989 | 2641444 |
| [water studies of inhabitants of refuse disposal sites in cairo. 1]. | in two waste disposal sites in cairo, drinking water samples from springs, drinking vessels and kitchens were investigated. total germ count lay seldom below one thousand and ten million pro ml. apart from various faecal germs, aeromonas hydrophila was also found. the high occurrence of diarrhoeal diseases among the inhabitants of the waste disposal sites can be traced with certainty to the extremely bad drinking water quality. | 1989 | 2641445 |
| the germ-spectrum problem and antibiotic-resistance in bida and minna hospitals--nigeria. | in the present paper the occurrence of different species of pseudomonas, of acinetobacter calcoaceticum var. anitratum and aeromonas hydrophila in hospitals is described. the possible human pathogenic significance is discussed. | 1989 | 2599385 |
| extracellular secretion of cloned aerolysin and phospholipase by aeromonas salmonicida. | the promoterless structural genes for aerolysin and the extracellular phospholipase of aeromonas hydrophila were inserted into a multi-host-range expression vector and transferred into aeromonas salmonicida and escherichia coli. in both species, gene expression was under the control of the inducible tac promoter of the vector. neither the phospholipase nor the aerolysin was released by intact e. coli. instead, both proteins accumulated in the periplasm, leading to reduced growth and eventual cel ... | 1989 | 2708312 |
| haemagglutinating activity of aeromonas spp. from different sources; attempted use as a typing system. | the haemagglutinating ability of 141 isolates of aeromonas spp. for human, horse and guinea-pig erythrocytes was examined. although the majority of isolates (136/141) agglutinated human group o erythrocytes, all the eight possible patterns of agglutination were observed. haemagglutination of human group o erythrocytes, but not horse or guinea-pig, was associated with the ability to agglutinate yeast cells (saccharomyces) and with aggregation in a low concentration of ammonium sulphate. haemagglu ... | 1989 | 2649385 |
| bacterial growth and toxin production in ileostomy effluents. | escherichia coli (2), vibrio cholerae (2) and aeromonas sobria (1) strains were examined for their ability to grow and produce toxins in samples of ileostomy fluid. three categories of response were observed: no detectable growth, growth without detectable toxin, and growth with detectable toxin. clear differences were apparent between samples of ileostomy fluid obtained from different individuals and between samples obtained from the same individual at different times. the patterns of response ... | 1989 | 2649680 |
| characterization of aeromonas salmonicida mutants with low-level resistance to multiple antibiotics. | aeromonas salmonicida mutants selected for low-level resistance to small-molecular-mass antibiotics occur at frequencies that suggest point mutations and exhibit pleiotropic effects such as a multiple low-level antibiotic resistance, changes in outer membrane protein profiles, and loss of major exoprotease activity. multiple low-level resistance appeared as the result of decreased outer membrane permeability associated with a change from a 38.5- to a 37-kilodalton (kda) outer membrane protein. t ... | 1989 | 2653212 |
| enteroaggregative escherichia coli associated with persistent diarrhea in a cohort of rural children in india. | a cohort of 452 rural children was followed longitudinally for 13 mo to ascertain the role of hep-2 cell adherent escherichia coli and other pathogens in causing acute (less than or equal to 14 d) and persistent (greater than 14 d) diarrhea. aeromonas, campylobacter jejuni, e. coli manifesting localized adherence to hep-2 cells and enterotoxigenic e. coli were significantly associated with acute diarrhea. e. coli strains that exhibit aggregative adherence, so-called enteroaggregative e. coli, a ... | 1989 | 2656875 |