Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| alimentary tract bacteria isolated and identified with api-20e and molecular cloning techniques from australian tropical fruit flies, bactrocera cacuminata and b. tryoni. | bacteria were isolated from the crop and midgut of field collected bactrocera cacuminata (hering) and bactrocera tryoni (froggatt) (diptera: tephritidae). two methods were used, firstly isolation onto two types of bacteriological culture media (pyea and tsa) and identification using the api-20e diagnostic kit, and secondly, analysis of samples using the 16s rrna gene molecular diagnostic method. using the api-20e method, 10 genera and 17 species of bacteria in the family enterobacteriaceae were ... | 2010 | 20883132 |
| the nonribosomal peptide synthetase enzyme ddad tethers n(β)-fumaramoyl-l-2,3-diaminopropionate for fe(ii)/α-ketoglutarate-dependent epoxidation by ddac during dapdiamide antibiotic biosynthesis. | the gene cluster from pantoea agglomerans responsible for biosynthesis of the dapdiamide antibiotics encodes an adenylation-thiolation didomain protein, ddad, and an fe(ii)/α-ketoglutarate-dependent dioxygenase homologue, ddac. here we show that ddad, a nonribosomal peptide synthetase module, activates and sequesters n(β)-fumaramoyl-l-2,3-diaminopropionate as a covalently tethered thioester for subsequent oxidative modification of the fumaramoyl group. ddac catalyzes fe(ii)- and α-ketoglutarate- ... | 2010 | 20945916 |
| genome sequence of the biocontrol agent pantoea vagans strain c9-1. | pantoea vagans is a gram-negative enterobacterial plant epiphyte of a broad range of plants. here we report the 4.89-mb genome sequence of p. vagans strain c9-1 (formerly pantoea agglomerans), which is commercially registered for biological control of fire blight, a disease of pear and apple trees caused by erwinia amylovora. | 2010 | 20952567 |
| etiology and antibacterial susceptibility pattern of community-acquired bacterial ocular infections in a tertiary eye care hospital in south india. | to identify the etiology, incidence and prevalence of ocular bacterial infections, and to assess the in vitro susceptibility of these ocular bacterial isolates to commonly used antibiotics. | 2010 | 20952834 |
| biodegradation of polyether algal toxins--isolation of potential marine bacteria. | marine algal toxins such as brevetoxins, okadaic acid, yessotoxin, and ciguatoxin are polyether compounds. the fate of polyether toxins in the aqueous phase, particularly bacterial biotransformation of the toxins, is poorly understood. an inexpensive and easily available polyether structural analog salinomycin was used for enrichment and isolation of potential polyether toxin degrading aquatic marine bacteria from florida bay area, and from red tide endemic sites in the south florida gulf coast. ... | 2010 | 20954040 |
| new blue pigment produced by pantoea agglomerans and its production characteristics at various temperatures. | a bacterium capable of producing a deep blue pigment was isolated from the environment and identified as pantoea agglomerans. the pigment production characteristics of the bacterium under various conditions were studied. the optimal agar plate ingredients for pigment production by the bacterium were first studied: the optimal ingredients were 5 g/liter glucose, 10 g/liter tryptic soy broth, and 40 g/liter glycerol at ph 6.4. bacterial cells grew on the agar plate during the incubation, while the ... | 2010 | 20971865 |
| development of an efficient real-time quantitative pcr protocol for detection of xanthomonas arboricola pv. pruni in prunus species. | xanthomonas arboricola pv. pruni, the causal agent of bacterial spot disease of stone fruit, is considered a quarantine organism by the european union and the european and mediterranean plant protection organization (eppo). the bacterium can undergo an epiphytic phase and/or be latent and can be transmitted by plant material, but currently, only visual inspections are used to certify plants as being x. arboricola pv. pruni free. a novel and highly sensitive real-time taqman pcr detection protoco ... | 2010 | 21037298 |
| development of an efficient real-time quantitative pcr protocol for detection of xanthomonas arboricola pv. pruni in prunus species. | xanthomonas arboricola pv. pruni, the causal agent of bacterial spot disease of stone fruit, is considered a quarantine organism by the european union and the european and mediterranean plant protection organization (eppo). the bacterium can undergo an epiphytic phase and/or be latent and can be transmitted by plant material, but currently, only visual inspections are used to certify plants as being x. arboricola pv. pruni free. a novel and highly sensitive real-time taqman pcr detection protoco ... | 2010 | 21037298 |
| the bacterial community in 'taberna' a traditional beverage of southern mexico. | to characterize the bacterial community of taberna, an alcoholic traditional beverage from the southern part of mexico produced by the fermentation of the coyol palm sap (acrocomia aculeate). | 2010 | 21039665 |
| micro ecosystems from feed industry surfaces: a survival and biofilm study of salmonella versus host resident flora strains. | the presence of salmonella enterica serovars in feed ingredients, products and processing facilities is a well recognized problem worldwide. in norwegian feed factories, strict control measures are implemented to avoid establishment and spreading of salmonella throughout the processing chain. there is limited knowledge on the presence and survival of the resident microflora in feed production plants. information on interactions between salmonella and other bacteria in feed production plants and ... | 2010 | 21044298 |
| a case of pantoea endophthalmitis. | a previously healthy 50-year-old man was transferred to our hospital for evaluation of acute inflammation in his right eye after ocular trauma while using a grass mower. slit lamp examination showed 1 mm-length full thickness corneal laceration without leakage, 4+ cells and inflammatory membrane in the anterior chamber, 10% hypopyon, posterior synechiae formation, and cataract change. upon orbital computerized tomography, a metallic intraocular foreign body in the lens was identified. vitrectomy ... | 2010 | 21052515 |
| rmtd2, a new allele of a 16s rrna methylase gene, has been present in enterobacteriaceae isolates from argentina for more than a decade. | the first allele of a 16s rrna methyltransferase gene, rmtd2, conferring very high resistance to all clinically available aminoglycosides, was detected in 7/1,064 enterobacteria collected in 2007. rmtd2 was located on a conjugative plasmid in a tn2670-like element inside a structure similar to that of rmtd1 but probably having an independent assembly. rmtd2 has been found since 1996 to 1998 mainly in enterobacter and citrobacter isolates, suggesting a possible reservoir in these genera. this pre ... | 2010 | 21078935 |
| rmtd2, a new allele of a 16s rrna methylase gene, has been present in enterobacteriaceae isolates from argentina for more than a decade. | the first allele of a 16s rrna methyltransferase gene, rmtd2, conferring very high resistance to all clinically available aminoglycosides, was detected in 7/1,064 enterobacteria collected in 2007. rmtd2 was located on a conjugative plasmid in a tn2670-like element inside a structure similar to that of rmtd1 but probably having an independent assembly. rmtd2 has been found since 1996 to 1998 mainly in enterobacter and citrobacter isolates, suggesting a possible reservoir in these genera. this pre ... | 2010 | 21078935 |
| paenibacillus polymyxa jb05-01-1 and its perspectives for food conservation and medical applications. | the aim of this study was to isolate a novel bacterial strain with strong and broad spectrum antibacterial activity from a livestock feed prebiotic supplement. a novel strain, termed paenibacillus polymyxa jb05-01-1, was isolated using traditional microbiological methods and identified on the basis of its phenotypic and biochemical properties as well as its 16s rrna gene sequence. this strain was able to inhibit growth of gram-negative bacteria including escherichia coli rr1, pseudomonas fluores ... | 2010 | 21132276 |
| water microbiology. bacterial pathogens and water. | water is essential to life, but many people do not have access to clean and safe drinking water and many die of waterborne bacterial infections. in this review a general characterization of the most important bacterial diseases transmitted through water-cholera, typhoid fever and bacillary dysentery-is presented, focusing on the biology and ecology of the causal agents and on the diseases' characteristics and their life cycles in the environment. the importance of pathogenic escherichia coli str ... | 2010 | 21139855 |
| characterization of a broad-host-range flagellum-dependent phage that mediates high-efficiency generalized transduction in, and between, serratia and pantoea. | a phage (phiot8) isolated on serratia sp. atcc 39006 was shown to be flagellum-dependent, and to mediate generalized transduction with high efficiency (up to 10(-4) transductants per p.f.u.). phiot8 was shown to have a broad host range because it also infected a strain of pantoea agglomerans isolated from the rhizosphere. transduction of plasmid-borne antibiotic resistance between the two bacterial genera was demonstrated, consistent with purported ecological roles of phages in dissemination of ... | 2010 | 19778959 |
| transgenesis and paratransgenesis to control insect-borne diseases: current status and future challenges. | insect-borne diseases cause significant human morbidity and mortality. current control and preventive methods against vector-borne diseases rely mainly on insecticides. the emergence of insecticide resistance in many disease vectors highlights the necessity to develop new strategies to control these insects. vector transgenesis and paratransgenesis are novel strategies that aim at reducing insect vectorial capacity, or seek to eliminate transmission of pathogens such as plasmodium sp., trypanoso ... | 2010 | 19819346 |
| transgenesis and paratransgenesis to control insect-borne diseases: current status and future challenges. | insect-borne diseases cause significant human morbidity and mortality. current control and preventive methods against vector-borne diseases rely mainly on insecticides. the emergence of insecticide resistance in many disease vectors highlights the necessity to develop new strategies to control these insects. vector transgenesis and paratransgenesis are novel strategies that aim at reducing insect vectorial capacity, or seek to eliminate transmission of pathogens such as plasmodium sp., trypanoso ... | 2010 | 19819346 |
| isolation and characterisation of bacterial colonies from seeds and in vitro cultures of fraxinus spp. from italian sites. | culturable bacteria were isolated from seeds, embryos and contaminated in vitro cultures of ash (fraxinus excelsior l., f. ornus l. and f. angustifolia l.) and were identified using morphological and molecular analyses. fourteen morphologically distinct isolates were recovered from seeds of fraxinus spp. 16s rdna sequencing categorised these isolates into ten separate genera. three strains isolated from contaminated in vitro cultures, pantoea agglomerans, staphylococcus succinus and aerococcus v ... | 2011 | 21143738 |
| clostridiaceae and enterobacteriaceae as active fermenters in earthworm gut content. | the earthworm gut provides ideal in situ conditions for ingested heterotrophic soil bacteria capable of anaerobiosis. high amounts of mucus- and plant-derived saccharides such as glucose are abundant in the earthworm alimentary canal, and high concentrations of molecular hydrogen (h(2)) and organic acids in the alimentary canal are indicative of ongoing fermentations. thus, the central objective of this study was to resolve potential links between fermentations and active fermenters in gut conte ... | 2011 | 20613788 |
| clostridiaceae and enterobacteriaceae as active fermenters in earthworm gut content. | the earthworm gut provides ideal in situ conditions for ingested heterotrophic soil bacteria capable of anaerobiosis. high amounts of mucus- and plant-derived saccharides such as glucose are abundant in the earthworm alimentary canal, and high concentrations of molecular hydrogen (h(2)) and organic acids in the alimentary canal are indicative of ongoing fermentations. thus, the central objective of this study was to resolve potential links between fermentations and active fermenters in gut conte ... | 2011 | 20613788 |
| mechanistically compatible mixtures of bacterial antagonists improve biological control of fire blight of pear. | mixtures of biological control agents can be superior to individual agents in suppressing plant disease, providing enhanced efficacy and reliability from field to field relative to single biocontrol strains. nonetheless, the efficacy of combinations of pseudomonas fluorescens a506, a commercial biological control agent for fire blight of pear, and pantoea vagans strain c9-1 or pantoea agglomerans strain eh252 rarely exceeds that of individual strains. a506 suppresses growth of the pathogen on fl ... | 2011 | 20839962 |
| bacterial and fungal communities of wilted italian ryegrass silage inoculated with and without lactobacillus rhamnosus or lactobacillus buchneri. | to understand the effects of lactic acid bacteria (lab) inoculation on fermentation products, aerobic stability and microbial communities of silage. | 2011 | 21204884 |
| extended-spectrum beta-lactamase gene sequences in gram-negative saprophytes on retail organic and nonorganic spinach. | a substantial proportion of infections caused by drug-resistant gram-negative bacteria (gnb) in community and health care settings are recognized to be caused by evolutionarily related gnb strains. their global spread has been suggested to occur due to human activities, such as food trade and travel. these multidrug-resistant gnb pathogens often harbor mobile drug resistance genes that are highly conserved in their sequences. because they appear across different gnb species, these genes may have ... | 2011 | 21216903 |
| the soxrs response of escherichia coli is directly activated by redox-cycling drugs rather than by superoxide. | when escherichia coli is exposed to redox-cycling drugs, its soxr transcription factor is activated by oxidation of its [2fe-2s] cluster. in aerobic cells these drugs generate superoxide, and because superoxide dismutase (sod) is a member of the soxrs regulon, superoxide was initially thought to be the activator of soxr. its many-gene regulon was therefore believed to comprise a defence against superoxide stress. however, we found that abundant superoxide did not effectively activate soxr in an ... | 2011 | 21226770 |
| comprehensive approaches to molecular biomarker discovery for detection and identification of cronobacter spp. (enterobacter sakazakii) and salmonella spp. | cronobacter spp. (formerly enterobacter sakazakii) and salmonella spp. are increasingly implicated internationally as important microbiological contaminants in low-moisture food products, including powdered infant formula. estimates indicate that 40 to 80% of infants infected with cronobacter sakazakii and/or salmonella in the united states may not survive the illness. a systematic approach, combining literature-based data mining, comparative genome analysis, and the direct sequencing of pcr pro ... | 2011 | 21239552 |
| multiple treatment meta-analysis of products evaluated for control of fire blight in the eastern united states. | the aim of this analysis was to estimate the effect sizes and consistency of products evaluated for fire blight control in the eastern united states over the last decade. because only 3% of the 69 studies published from 2000 to 2008 explicitly presented a measure of within-study variability, a method for estimating the least significant difference (lsd) and, hence the sampling variance, for studies with at least two significant mean separations in the presented mean multiple comparisons was deve ... | 2011 | 21244224 |
| genome mining in streptomyces. elucidation of the role of baeyer-villiger monooxygenases and non-heme iron-dependent dehydrogenase/oxygenases in the final steps of the biosynthesis of pentalenolactone and neopentalenolactone. | the pentalenolactone biosynthetic gene clusters have been cloned and sequenced from two known producers of the sesquiterpenoid antibiotic pentalenolactone, streptomyces exfoliatus uc5319 and streptomyces arenae tu¨469. the recombinant enzymes pene and pnte, from s. exfoliatus and s. arenae, respectively, catalyze the flavin-dependent baeyer-villiger oxidation of 1-deoxy-11-oxopentalenic acid (7) to pentalenolactone d (8). recombinant pend, pntd, and ptld, the latter from streptomyces avermitilis ... | 2011 | 21250661 |
| differences in bacterial diversity of host-associated populations of phylloxera notabilis pergande (hemiptera: phylloxeridae) in pecan and water hickory. | host-associated differentiation (had) is the presence of genetically divergent, host-associated populations. it has been suggested that microbial symbionts of insect herbivores may play a role in had by allowing their insect hosts to use different plant species. the objective of this study was to document if host-associated populations of phylloxera notabilis pergande (hemiptera: phylloxeridae) in pecan and water hickory corresponded with differences in the composition of their associated bacter ... | 2011 | 21261774 |
| antibiotic resistance in bacteria isolated from vegetables with regards to the marketing stage (farm vs. supermarket). | the aim of this study was to elucidate whether and to what extent fresh produce from germany plays a role as a carrier and reservoir of antibiotic resistant bacteria. for this purpose, 1001 vegetables (fruit, root, bulbous vegetables, salads and cereals) were collected from 13 farms and 11 supermarkets in germany and examined bacteriologically. phenotypic resistance of enterobacter cloacae (n=172); enterobacter gergoviae (n=92); pantoea agglomerans (n=96); pseudomonas aeruginosa (n=295); pseudom ... | 2011 | 21700353 |
| comparative analysis of the xopd type iii secretion (t3s) effector family in plant pathogenic bacteria. | xopd is a type iii effector protein that is required for xanthomonas campestris pathovar vesicatoria (xcv) growth in tomato. it is a modular protein consisting of an n-terminal dna-binding domain, two ethylene-responsive element binding factor-associated amphiphilic repression (ear) transcriptional repressor motifs and a c-terminal small ubiquitin-related modifier (sumo) protease. in tomato, xopd functions as a transcriptional repressor, resulting in the suppression of defence responses at late ... | 2011 | 21726373 |
| analysis of al-2 mutations in neurospora. | the orange pigmentation of the fungus neurospora crassa is due to the accumulation of the xanthophyll neurosporaxanthin and precursor carotenoids. two key reactions in the synthesis of these pigments, the formation of phytoene from geranylgeranyl pyrophosphate and the introduction of +¦ cycles in desaturated carotenoid products, are catalyzed by two domains of a bifunctional protein, encoded by the gene al-2. we have determined the sequence of nine al-2 mutant alleles and analyzed the carotenoid ... | 2011 | 21818281 |
| impact of derepressed ampc {beta}-lactamase act-9 on the clinical efficacy of ertapenem. | an in vivo development of pantoea agglomerans mutants (isolates pa2 to pa4) with reduced ertapenem susceptibility from that of isolate pa1 was associated with an inadequate clinical response to ertapenem therapy. all four isolates harbored the bla(act-9) ampc +¦-lactamase gene. however, a loss-of-function mutation in the ampd gene in pa2 to pa4, but not pa1, led to derepressed act-9. the reduced ertapenem susceptibility caused by derepressed act-9 was confirmed with an ampd knockout mutant of pa ... | 2011 | 21690276 |
| Sharing of quorum-sensing signals and role of interspecies communities in a bacterial plant disease. | Pathogenic bacteria interact not only with the host organism but most probably also with the resident microbial flora. In the knot disease of the olive tree (Olea europaea), the causative agent is the bacterium Pseudomonas savastanoi pv. savastanoi (Psv). Two bacterial species, namely Pantoea agglomerans and Erwinia toletana, which are not pathogenic and are olive plant epiphytes and endophytes, have been found very often to be associated with the olive knot. We identified the chemical signals t ... | 2011 | 21677694 |
| septic arthritis due to cellulosimicrobium cellulans. | cellulosimicrobium cellulans has been reported as a rare cause of human pathogenesis. infections mainly occur in immunocompromised patients and very often are associated with a foreign body. we report the first case of septic arthritis caused by c. cellulans in an immunocompetent patient. our patient suffered a penetrating palm tree thorn injury to his left knee 8 weeks before admission. although no foreign objects were found, they were suspected because previous reports suggest a frequent assoc ... | 2011 | 21998421 |
| vim and imp metallo-β-lactamases and other extended-spectrum β-lactamases in escherichia coli and klebsiella pneumoniae from environmental samples in a tunisian hospital. | an extremely drug-resistant enterobacteriaceae species emerged in kasserine hospital, tunisia between 2009 and 2010 causing a local outbreak. we aimed to characterize extended-spectrum β-lactamase (esbl) and metallo-β-lactamase (mbl)-producing enterobacteriaceae from the hospital environment. swabs were collected from ten different wards from kasserine hospital, tunisia. a total of 46 isolates were cultured onto macconkey agar supplemented with ceftazidime to select for esbl-producing enterobact ... | 2011 | 21917010 |
| erwinia amylovora novel plasmid pei70: complete sequence, biogeography, and role in aggressiveness in the fire blight phytopathogen. | comparative genomics of several strains of erwinia amylovora, a plant pathogenic bacterium causal agent of fire blight disease, revealed that its diversity is primarily attributable to the flexible genome comprised of plasmids. we recently identified and sequenced in full a novel 65.8 kb plasmid, called pei70. annotation revealed a lack of known virulence-related genes, but found evidence for a unique integrative conjugative element related to that of other plant and human pathogens. comparative ... | 2011 | 22174857 |
| Salmonella biofilm formation on Aspergillus niger involves cellulose--chitin interactions. | Salmonella cycles between host and nonhost environments, where it can become an active member of complex microbial communities. The role of fungi in the environmental adaptation of enteric pathogens remains relatively unexplored. We have discovered that S. enterica Typhimurium rapidly attaches to and forms biofilms on the hyphae of the common fungus, Aspergillus niger. Several Salmonella enterica serovars displayed a similar interaction, whereas other bacterial species were unable to bind to the ... | 2011 | 22003399 |
| Comparative genomics of the type VI secretion systems of Pantoea and Erwinia species reveals the presence of putative effector islands that may be translocated by the VgrG and Hcp proteins. | ABSTRACT: | 2011 | 22115407 |
| A Microarray Biosensor for Multiplexed Detection of Microbes Using Grating-Coupled Surface Plasmon Resonance Imaging. | Grating-coupled surface plasmon resonance imaging (GCSPRI) utilizes an optical diffraction grating embossed on a gold-coated sensor chip to couple collimated incident light into surface plasmons. The angle at which this coupling occurs is sensitive to the capture of analyte at the chip surface. This approach permits the use of disposable biosensor chips that can be mass-produced at low cost and spotted in microarray format to greatly increase multiplexing capabilities. The current GCSPRI instrum ... | 2011 | 22029256 |
| A Microarray Biosensor for Multiplexed Detection of Microbes Using Grating-Coupled Surface Plasmon Resonance Imaging. | Grating-coupled surface plasmon resonance imaging (GCSPRI) utilizes an optical diffraction grating embossed on a gold-coated sensor chip to couple collimated incident light into surface plasmons. The angle at which this coupling occurs is sensitive to the capture of analyte at the chip surface. This approach permits the use of disposable biosensor chips that can be mass-produced at low cost and spotted in microarray format to greatly increase multiplexing capabilities. The current GCSPRI instrum ... | 2011 | 22029256 |
| Functional characterization of lipopolysaccharide derived from symbiotic bacteria in rice as a macrophage-activating substance. | Lipopolysaccharide derived from a symbiotic bacterium in wheat (Pantoea agglomerans, LPSp) has shown multiple positive effects, such as prophylactic, antiallergic and antitumour effects, without serious side-effects. LPSp has differential biological activities in comparison to other LPS, such as those from Escherichia coli (LPSe). The only difference between LPSp and LPSe is in the O-antigen polysaccharide structure (O-PS). This led us to the hypothesis that the O-PS structure would seem to part ... | 2011 | 21873161 |
| the impact of relative humidity and collection media on mycobacteriophage d29 aerosol. | this study was conducted to evaluate the effect of aerosol generation, methods of sampling, storage conditions and relative humidity on the culturability of the mycobacteriophage d29. the lytic phage d29 can kill mycobacterium tuberculosis and the phage aerosol can be treated as a potential tool for tuberculosis treatment. the culturability of d29 was tested using a test chamber designed for the bioaerosols research against three spray liquids (deionized water, pbs and normal saline), four colle ... | 2011 | 22194291 |
| lactobacillus reuteri-produced cyclic dipeptides quench agr-mediated expression of toxic shock syndrome toxin-1 in staphylococci. | the production of the staphylococcal exotoxin toxic shock syndrome toxin-1 (tsst-1) by staphylococcus aureus has been associated with essentially all cases of menstruation-associated toxic shock syndrome (tss). in this work, we show that the human vaginal isolate lactobacillus reuteri rc-14 produces small signaling molecules that are able to interfere with the staphylococcal quorum-sensing system agr, a key regulator of virulence genes, and repress the expression of tsst-1 in s. aureus mn8, a pr ... | 2011 | 21282650 |
| rapid identification of gram-negative bacteria with and without ctx-m extended-spectrum ß-lactamase from positive blood culture bottles by pcr followed by microchip gel electrophoresis. | we evaluated the usefulness of pcr analysis of the 16s-23s rrna gene internal transcribed spacer (its) and the ctx-m extended-spectrum ß-lactamase (esbl) followed by microchip gel electrophoresis (mge) for direct identification and ctx-m detection of gram-negative bacteria (gnb) from positive blood culture bottles. of 251 gnb isolated from blood cultures containing a single bacterium, 225 (90%) were correctly identified at the species level directly from positive blood culture bottles by compari ... | 2011 | 21289149 |
| characterization of acc deaminase-producing endophytic bacteria isolated from copper-tolerant plants and their potential in promoting the growth and copper accumulation of brassica napus. | one hundred cu-resistant-endophytic bacteria were isolated from cu-tolerant plants grown on cu mine wasteland, of which, eight cu-resistant and 1-aminocyclopropane-1-carboxylate (acc) deaminase-producing endophytic bacteria were obtained based on the acc deaminase activity of the bacteria and characterized with respect to metal resistance, production of acc deaminase, indole-3-acetic acid (iaa) as well as siderophores and mineral phosphate solubilization. ralstonia sp. j1-22-2, pantoea agglomera ... | 2011 | 21315404 |
| optimization of medium for indole-3-acetic acid production using pantoea agglomerans strain pvm. | to optimize the medium components for the production of indole-3-acetic acid (iaa) by isolated bacterium pantoea agglomerans strain pvm. | 2011 | 21332896 |
| characterization of bacillus subtilis hc8, a novel plant-beneficial endophytic strain from giant hogweed. | thirty endophytic bacteria were isolated from various plant species growing near saint-petersburg, russia. based on a screening for various traits, including plant-beneficial properties and dna fragment patterns, potential siblings were removed. the remaining isolates were taxonomically identified using 16s rdna sequences and potential human and plant pathogens were removed. the remaining strains were tested for their ability to promote radish root growth and to protect tomato plants against tom ... | 2011 | 21366893 |
| characterization of nuclear localization signals in the type iii effectors hsvg and hsvb of the gall-forming bacterium pantoea agglomerans. | hsvg and hsvb, two paralogous type iii effectors of the gall-forming bacteria pantoea agglomerans pv. gypsophilae and p. agglomerans pv. betae, determine host specificity on gypsophila and beet, respectively. they were previously shown to be dna-binding proteins imported into host and non-host nuclei and might act as transcriptional activators. sequence analysis of these effectors did not detect canonical nuclear localization signals (nlss), but two basic amino acid clusters designated putative ... | 2011 | 21372093 |
| spatiotemporal development of the bacterial community in a tubular longitudinal microbial fuel cell. | the spatiotemporal development of a bacterial community in an exoelectrogenic biofilm was investigated in sucrose-fed longitudinal tubular microbial fuel cell reactors, consisting of two serially connected modules. the proportional changes in the microbial community composition were assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (dgge) and dna sequencing in order to relate them to the performance and stability of the bioelectrochemical system. the reproducibility o ... | 2011 | 21400098 |
| bacteriophages limelight and limezero of pantoea agglomerans, belonging to the "phikmv-like viruses". | pantoea agglomerans is a common soil bacterium used in the biocontrol of fungi and bacteria but is also an opportunistic human pathogen. it has been described extensively in this context, but knowledge of bacteriophages infecting this species is limited. bacteriophages limezero and limelight of p. agglomerans are lytic phages, isolated from soil samples, belonging to the podoviridae and are the first pantoea phages of this family to be described. the double-stranded dna (dsdna) genomes (43,032 b ... | 2011 | 21421778 |
| improved resistance against botrytis cinerea by grapevine-associated bacteria that induce a prime oxidative burst and phytoalexin production. | bacteria such as pantoea agglomerans (pa-af2), bacillus subtilis (bs-271), acinetobacter lwoffii (al-113), and pseudomonas fluorescens (pf-ct2), originating from the vineyard, can induce defense responses and enhance resistance of grapevine against the fungal pathogen botrytis cinerea. the perception of these bacteria by plant cells or tissues in relation to their activities remains unknown. in this study, we examined the relationships between the activity of each bacterium to induce or prime so ... | 2011 | 21425931 |
| improvement of fitness and efficacy of a fire blight biocontrol agent via nutritional enhancement combined with osmoadaptation. | the efficacy of pseudomonas fluorescens eps62e in the biocontrol of erwinia amylovora was improved by a procedure of physiological adaptation to increase colonization and survival in the phytosphere of rosaceous plants. the procedure consisted of osmoadaptation (oa) and nutritional enhancement (ne). oa was based on saline stress and osmolyte amendment of the growth medium during inoculum preparation. ne consisted of addition of glycine and tween 80 to the formulation. ne and oa increased the gro ... | 2011 | 21441337 |
| unusual causes of peritonitis in a peritoneal dialysis patient: alcaligenes faecalis and pantoea agglomerans. | an 87 -year-old female who was undergoing peritoneal dialysis presented with peritonitis caused by alcaligenes faecalis and pantoea agglomerans in consecutive years. with the following report we discuss the importance of these unusual microorganisms in peritoneal dialysis patients. | 2011 | 21477370 |
| microbiology of animal bite wound infections. | the microbiology of animal bite wound infections in humans is often polymicrobial, with a broad mixture of aerobic and anaerobic microorganisms. bacteria recovered from infected bite wounds are most often reflective of the oral flora of the biting animal, which can also be influenced by the microbiome of their ingested prey and other foods. bacteria may also originate from the victim's own skin or the physical environment at the time of injury. our review has focused on bite wound infections in ... | 2011 | 21482724 |
| pantoea agglomerans pneumonia in a heart-lung transplant recipient: case report and a review of an emerging pathogen in immunocompromised hosts. | a. shubov, p. jagannathan, p. v. chin-hong. pantoea agglomerans pneumonia in a heart-lung transplant recipient: case report and a review of an emerging pathogen in immunocompromised hosts. transpl infect dis 2011. all rights reserved abstract: pantoea agglomerans is a gram-negative rod that is frequently found on the exterior of many plants, fruits, vegetables, and in soil, and it is used as a biopesticide in the agriculture industry. recent reports have implicated p. agglomerans in systemic inf ... | 2011 | 21504526 |
| oral administration of immunopotentiator from pantoea agglomerans 1 (ip-pa1) improves the survival of b16 melanoma-inoculated model mice. | to investigate the usefulness of the immunopotentiator from pantoea agglomerans 1 (ip-pa1) as a supportive drug in melanoma therapy, we analyzed the immunological effects of ip-pa1 on melanoma-inoculated model mice. oral administration of ip-pa1 increased the serum levels of tumor necrosis factor (tnf)-α at 2 h after the administration and interferon (ifn)-γ and il-12 at 12 h after the administration in naïve balb/ccrslc mice as evaluated by elisa. ip-pa1 did not affect the proliferation of mela ... | 2011 | 21512265 |
| enhancement of carotenoids biosynthesis in chlamydomonas reinhardtii by nuclear transformation using a phytoene synthase gene isolated from chlorella zofingiensis. | the isolation and characterization of the phytoene synthase gene from the green microalga chlorella zofingiensis (czpsy), involved in the first step of the carotenoids biosynthetic pathway, have been performed. czpsy gene encodes a polypeptide of 420 amino acids. a single copy of czpsy has been found in c. zofingiensis by southern blot analysis. heterologous genetic complementation in escherichia coli showed the ability of the predicted protein to catalyze the condensation of two molecules of ge ... | 2011 | 21519934 |
| a head-to-head comparison of eneamide and epoxyamide inhibitors of glucosamine-6-phosphate synthase from the dapdiamide biosynthetic pathway. | the dapdiamides make up a family of antibiotics that have been presumed to be cleaved in the target cell to enzyme-inhibitory n-acyl-2,3-diaminopropionate (dap) warheads containing two alternative electrophilic moieties. our prior biosynthetic studies revealed that an eneamide warhead is made first and converted to an epoxyamide via a three-enzyme branch pathway. here we provide a rationale for this logic. we report that the r,r-epoxyamide warhead is a more efficient covalent inactivator of gluc ... | 2011 | 21520904 |
| the t7-related pseudomonas putida phage ϕ15 displays virion-associated biofilm degradation properties. | formation of a protected biofilm environment is recognized as one of the major causes of the increasing antibiotic resistance development and emphasizes the need to develop alternative antibacterial strategies, like phage therapy. this study investigates the in vitro degradation of single-species pseudomonas putida biofilms, ppg1 and rd5pr2, by the novel phage ϕ15, a 't7-like virus' with a virion-associated exopolysaccharide (eps) depolymerase. phage ϕ15 forms plaques surrounded by growing opaqu ... | 2011 | 21526174 |
| effects of bacterial microflora of the lower digestive tract of free-range waterfowl on influenza virus activation. | proteolytic cleavage activation of influenza hemagglutinin (ha0) is required for cell entry via receptor mediated endocytosis. despite numerous studies describing bacterial protease mediated influenza a viral activation in mammals, very little is known about the role of intestinal bacterial flora of birds in hemagglutinin cleavage/activation. therefore, the cloaca of wild waterfowl was examined for 1) representative bacterial types and 2) their ability to cleave in 'trypsin-like' manner the prec ... | 2011 | 21531837 |
| classification of plant associated bacteria using rif, a computationally derived dna marker. | a dna marker that distinguishes plant associated bacteria at the species level and below was derived by comparing six sequenced genomes of xanthomonas, a genus that contains many important phytopathogens. this dna marker comprises a portion of the dnaa replication initiation factor (rif). unlike the rrna genes, dnaa is a single copy gene in the vast majority of sequenced bacterial genomes, and amplification of rif requires genus-specific primers. in silico analysis revealed that rif has equal or ... | 2011 | 21533033 |
| kinetics of bacterial potentiometric titrations: the effect of equilibration time on buffering capacity of pantoea agglomerans suspensions. | several recent studies have made use of continuous acid-base titration data to describe the surface chemistry of bacterial cells as a basis for accurately modelling metal adsorption to bacteria and other biomaterials of potential industrial importance. these studies do not share a common protocol; rather they titrate in different ph ranges and they use different stability criteria to define equilibration time during titration. in the present study we investigate the kinetics of bacterial titrati ... | 2011 | 21543082 |
| stereochemistry and mechanism of a microbial phenylalanine aminomutase. | the stereochemistry of a phenylalanine aminomutase (pam) on the andrimid biosynthetic pathway in pantoea agglomerans (pa) is reported. papam is a member of the 4-methylidene-1h-imidazol-5(4h)-one (mio)-dependent family of catalysts and isomerizes (2s)-α-phenylalanine to (3s)-β-phenylalanine, which is the enantiomer of the product made by the mechanistically similar aminomutase tcpam from taxus plants. the nh(2) and pro-(3s) hydrogen groups at c(α) and c(β), respectively, of the substrate are rem ... | 2011 | 21561099 |
| curtobacterium flaccumfaciens septic arthritis following puncture with a coxspur hawthorn thorn. | curtobacterium species are recognised plant pathogens. we report the first well-documented case of curtobacterium human infection, a child with septic arthritis following puncture with a coxspur hawthorn plant thorn. the organism isolated from synovial tissue and the plant thorn was identified as curtobacterium flaccumfaciens by 16s rrna gene sequence analysis. | 2011 | 21562106 |
| secretion of anti-plasmodium effector proteins from a natural isolate pantoea agglomerans using pelb and hlya secretion signals. | the insect-vectored disease malaria is a major world health problem. new control strategies are needed to supplement the current use of insecticides and medications. a genetic approach can be used to inhibit development of the malaria parasite (plasmodium sp.) in the mosquito host. we hypothesized that pantoea agglomerans, a bacterial symbiont of anopheles mosquitoes, could be engineered to express and secrete anti-plasmodium effector proteins, a strategy termed paratransgenesis. to this end, pl ... | 2011 | 21602368 |
| directed evolution of the nonribosomal peptide synthetase admk generates new andrimid derivatives in vivo. | many lead compounds in the search for new drugs derive from peptides and polyketides whose similar biosynthetic enzymes have been difficult to engineer for production of new derivatives. problems with generating multiple analogs in a single experiment along with lack of high-throughput methods for structure-based screening have slowed progress in this area. here, we use directed evolution and a multiplexed assay to screen a library of >14,000 members to generate three derivatives of the antibact ... | 2011 | 21609841 |
| characterization of a small cryptic plasmid from endophytic pantoea agglomerans and its use in the construction of an expression vector. | a circular cryptic plasmid named ppaga (2,734 bp) was isolated from pantoea agglomerans strain ege6 (an endophytic bacterial isolate from eucalyptus). sequence analysis revealed that the plasmid has a g+c content of 51% and contains four potential orfs, 238(a), 250(b), 131(c), and 129(d) amino acids in length without homology to known proteins. the shuttle vector plgm1 was constructed by combining the ppaga plasmid with pgfpmut3.0 (which harbors a gene encoding green fluorescent protein, gfp), a ... | 2011 | 21637551 |
| rhizosphere competent pantoea agglomerans enhances maize (zea mays) and chickpea (cicer arietinum l.) growth, without altering the rhizosphere functional diversity. | plant growth promoting pantoea agglomerans nbrisrm (nbrisrm) was able to produce 60.4 μg/ml indole acetic acid and solubilize 77.5 μg/ml tri-calcium phosphate under in vitro conditions. addition of 2% nacl (w/v) in the media induced the iaa production and phosphate solubilization by 11% and 7%, respectively. for evaluating the plant growth promotory effect of nbrisrm inoculation a micro plot trial was conducted using maize and chickpea as host plants. the results revealed significant increase in ... | 2011 | 21638110 |
| carotenoid ╬▓-ring hydroxylase and ketolase from marine bacteria-promiscuous enzymes for synthesizing functional xanthophylls. | marine bacteria belonging to genera paracoccus and brevundimonas of the ╬▒-proteobacteria class can produce côéäôéç-type dicyclic carotenoids containing two ╬▓-end groups (╬▓ rings) that are modified with keto and hydroxyl groups. these bacteria produce astaxanthin, adonixanthin, and their derivatives, which are ketolated by carotenoid ╬▓-ring 4(4')-ketolase (4(4')-oxygenase; crtw) and hydroxylated by carotenoid ╬▓-ring 3(3')-hydroxylase (crtz). in addition, the genus brevundimonas possesses a g ... | 2011 | 21673887 |
| 16s rdna-based phylogeny of non-symbiotic bacteria of entorno-pathogenic nematodes from infected insect cadavers. | using 16s rdna gene sequencing technique, three different species of non-symbiotic bacteria of entomopatho-genic nematodes (epns) (steinernema sp. and heterorhabditis sp.) were isolated and identified from infected insect cadavers {galleria mellonella larvae) after 48-hour post infections. sequence similarity analysis revealed that the strains srk3, srk4 and srk5 belong to ochrobactrum cytisi, schineria larvae and ochrobactrum anthropi, respectively. the isolates o. anthropi and s. larvae were f ... | 2011 | 21802047 |
| molecular and physiological properties of bacteriophages from north america and germany affecting the fire blight pathogen erwinia amylovora. | for possible control of fire blight affecting apple and pear trees, we characterized erwinia amylovora phages from north america and germany. the genome size determined by electron microscopy (em) was confirmed by sequence data and major coat proteins were identified from gel bands by mass spectroscopy. by their morphology from em data, fea1h and fea100 were assigned to the podoviridae and fea104 and fea116 to the myoviridae. host ranges were essentially confined to e. amylovora, strains of the ... | 2011 | 21791029 |
| metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist pantoea vagans c9-1. | pantoea vagans is a commercialized biological control agent used against the pome fruit bacterial disease fire blight, caused by erwinia amylovora. compared to other biocontrol agents, relatively little is currently known regarding pantoea genetics. better understanding of antagonist mechanisms of action and ecological fitness is critical to improving efficacy. | 2011 | 21789243 |
| novel virulent and broad host range erwinia amylovora bacteriophages reveal a high degree of mosaicism and relationship to enterobacteriaceae phages. | a diverse set of 24 novel phages infecting the fire blight pathogen erwinia amylovora was isolated from fruit production environments in switzerland. based on initial screening, four phages (l1, m7, s6, and y2) with broad host ranges were selected for detailed characterization and genome sequencing. phage l1 is a member of the podoviridae, with a 39.3 kbp genome featuring invariable genome ends with direct terminal repeats. phage s6, another podovirus was also found to possess direct terminal re ... | 2011 | 21764969 |
| rapid concentration and molecular enrichment approach for sensitive detection of escherichia coli/shigella in potable water samples. | in this work, we used a rapid, simple, and efficient concentration and recovery procedure combined with a dna enrichment method (dubbed crename), that we coupled to an escherichia coli/shigella-specific real-time pcr (rtpcr) assay targeting the tuf gene, to sensitively detect e. coli/shigella in water. this integrated method was compared to the usepa culture-based method 1604 on mi agar in terms of analytical specificity, ubiquity, detection limit, and rapidity. none of the 179 non-e. coli/shige ... | 2011 | 21764965 |
| validation of the intact zwittermicin a biosynthetic gene cluster and discovery of a complementary resistance mechanism in bacillus thuringiensis. | zwittermicin a (zma) is a hybrid polyketide-nonribosomal peptide produced by certain bacillus cereus group strains. it displays broad-spectrum antimicrobial activity. its biosynthetic pathway in b. cereus has been proposed through analysis of the nonribosomal peptide synthetase (nrps) and polyketide synthase (pks) modules involved in zma biosynthesis. in this study, we constructed a bacterial artificial chromosome (bac) library from bacillus thuringiensis subsp. kurstaki strain ybt-1520 genomic ... | 2011 | 21730118 |
| mouse model of hypersensitivity pneumonitis after inhalation exposure to different microbial antigens associated with organic dusts. | the aim of this study was to reproduce in laboratory conditions hypersensitivity pneumonitis (hp) pathogenesis in a new animal model predictive of the human response, and to select the microbial antigen associated with organic dust that exerts the strongest pathogenic effect on the respiratory organ. to achieve this goal, mice of the strain c57bl/6j prone to fibrosis were exposed for 1 hour daily up to 28 days to the inhalation of aerosols of each of the 5 microbial components of organic dusts w ... | 2011 | 21736281 |
| bacterial community composition of biological degreasing systems and health risk assessment for workers. | biological degreasing system is a new technology based on the degradation capabilities of microorganisms to remove oil, grease, or lubricants from metal parts. no data is available about the potential biological health hazards in such system. thus, a health risk assessment linked to the bacterial populations present in this new degreasing technology is, therefore, necessary for workers. we performed both cultural and molecular approaches in several biological degreasing systems for various indus ... | 2011 | 21698403 |
| antibiosis activity of pantoea agglomerans biocontrol strain e325 against erwinia amylovora on apple flower stigmas. | pantoea agglomerans e325, the active ingredient in a commercial product for fire blight control, was previously shown in vitro to produce a unique alkaline- and phosphate-sensitive antibiotic specific to erwinia amylovora. antibiosis was evaluated as a mode of antagonism on flower stigmas using two antibiosis-deficient mutants. on king's medium b, mutants e325ad1 and e325ad2 have stable smooth-butyrous or hypermucoid colony morphologies, respectively, and the parental strain e325 exhibits phenot ... | 2011 | 21679036 |
| quorum sensing and bacterial pathogenicity: from molecules to disease. | quorum sensing in prokaryotic biology refers to the ability of a bacterium to sense information from other cells in the population when they reach a critical concentration (i.e. a quorum) and communicate with them. the "language" used for this intercellular communication is based on small, self-generated signal molecules called as autoinducers. quorum sensing is thought to afford pathogenic bacteriaa mechanism to minimize host immune responses by delaying theproduction of tissue-damaging virulen ... | 2011 | 21701655 |
| exposure to bioaerosols in the selected agricultural facilities of the ukraine and poland - a review. | the aim of this work was to review the studies on bioaerosols which were carried out in the years 1972-2009 in following branches of agricultural industry in the ukraine and poland: animal farms for cows and pigs, animal feed facilities, production of biofuel from rape, herb farms and herb processing facilities. in all facilities were determined: concentration of dust and microorganisms in the air and species composition of microflora. moreover, in polish animal farms, herb farms and herb proces ... | 2011 | 21736265 |
| insights into cross-kingdom plant pathogenic bacteria. | plant and human pathogens have evolved disease factors to successfully exploit their respective hosts. phytopathogens utilize specific determinants that help to breach reinforced cell walls and manipulate plant physiology to facilitate the disease process, while human pathogens use determinants for exploiting mammalian physiology and overcoming highly developed adaptive immune responses. emerging research, however, has highlighted the ability of seemingly dedicated human pathogens to cause plant ... | 2011 | 24710301 |
| a selective chromogenic plate, yeca, for the detection of pathogenic yersinia enterocolitica: specificity, sensitivity, and capacity to detect pathogenic y. enterocolitica from pig tonsils. | a new selective chromogenic plate, yeca, was tested for its specificity, sensitivity, and accuracy to detect pathogenic y. enterocolitica from pig tonsils. we tested a panel of 26 bacterial strains on yeca and compared it to pca, cin, and yecm media. detection of pathogenic y. enterocolitica was carried out on 50 pig tonsils collected in one slaughter house. enrichment was done in psb and itc broths. streaking on yeca and cin was done in direct, after 24h incubation of itc, after 48h incubation ... | 2011 | 22567328 |
| comparison of bruker biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometer to bd phoenix automated microbiology system for identification of gram-negative bacilli. | we compared the bd phoenix automated microbiology system to the bruker biotyper (version 2.0) matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry (ms) system for identification of gram-negative bacilli, using biochemical testing and/or genetic sequencing to resolve discordant results. the bd phoenix correctly identified 363 (83%) and 330 (75%) isolates to the genus and species level, respectively. the bruker biotyper correctly identified 408 (93%) and 360 (82 ... | 2011 | 21209160 |
| advances in electronic-nose technologies developed for biomedical applications. | the research and development of new electronic-nose applications in the biomedical field has accelerated at a phenomenal rate over the past 25 years. many innovative e-nose technologies have provided solutions and applications to a wide variety of complex biomedical and healthcare problems. the purposes of this review are to present a comprehensive analysis of past and recent biomedical research findings and developments of electronic-nose sensor technologies, and to identify current and future ... | 2011 | 22346620 |
| auxin and plant-microbe interactions. | microbial synthesis of the phytohormone auxin has been known for a long time. this property is best documented for bacteria that interact with plants because bacterial auxin can cause interference with the many plant developmental processes regulated by auxin. auxin biosynthesis in bacteria can occur via multiple pathways as has been observed in plants. there is also increasing evidence that indole-3-acetic acid (iaa), the major naturally occurring auxin, is a signaling molecule in microorganism ... | 2011 | 21084388 |
| reiterative recombination for the in vivo assembly of libraries of multigene pathways. | the increasing sophistication of synthetic biology is creating a demand for robust, broadly accessible methodology for constructing multigene pathways inside of the cell. due to the difficulty of rationally designing pathways that function as desired in vivo, there is a further need to assemble libraries of pathways in parallel, in order to facilitate the combinatorial optimization of performance. while some in vitro dna assembly methods can theoretically make libraries of pathways, these techni ... | 2011 | 21876185 |
| multi-locus variable-number tandem repeat profiling of salmonella enterica serovar typhi isolates from blood cultures and gallbladder specimens from makassar, south-sulawesi, indonesia. | multi-locus variable-number tandem repeat analysis differentiated 297 salmonella enterica serovar typhi blood culture isolates from makassar in 76 genotypes and a single unique s. typhi genotype was isolated from the cholecystectomy specimens of four patients with cholelithiasis. the high diversity in s. typhi genotypes circulating in makassar indicates that the number of carriers could be very large, which may complicate disease prevention and control. | 2011 | 21949819 |
| mutation of l-2,3-diaminopropionic acid synthase genes blocks staphyloferrin b synthesis in staphylococcus aureus. | staphylococcus aureus synthesizes two siderophores, staphyloferrin a and staphyloferrin b, that promote iron-restricted growth. previous work on the biosynthesis of staphyloferrin b has focused on the role of the synthetase enzymes, encoded from within the sbna-i operon, which build the siderophore from the precursor molecules citrate, alpha-ketoglutarate and l-2,3-diaminopropionic acid. however, no information yet exists on several other enzymes, expressed from the biosynthetic cluster, that ar ... | 2011 | 21906287 |
| Bacterial skin flora variation and in vitro inhibitory activity against Saprolegnia parasitica in brown and rainbow trout. | Variations in the number and diversity of bacteria from the skin of brown trout Salmo trutta L. and rainbow trout Oncorhynchus mykiss Walbaum were surveyed from different rivers and fish farms in northern Spain. In addition to determining bacterial populations in skin samples of healthy fish, bacterial populations were determined from skin lesions (of brown trout only) infected with Saprolegnia parasitica, the causal agent of saprolegniosis. Mean bacterial counts from skin lesions of brown trout ... | 2011 | 22013752 |
| spm43.1 contributes to acid-resistance of non-symplasmata-forming cells in pantoea agglomerans ys19. | pantoea agglomerans ys19 is a rice endophytic bacterium characterized to form multicellular biofilm-like structures called symplasmata. phenotypic distinctions between symplasmata-forming cells and planktonic cells are crucial for understanding ys19's survival strategies. in this study, a 43.1 kda protein spm43.1 was identified to show significant resistance to the aggregation effect caused by denaturing acidic conditions. maldi-tof analysis data indicated that it is a maltose-binding protein ho ... | 2011 | 22134847 |
| a novel hydrolase identified by genomic-proteomic analysis of phenylurea herbicide mineralization by variovorax sp. strain srs16. | the soil bacterial isolate variovorax sp. strain srs16 mineralizes the phenylurea herbicide linuron. the proposed pathway initiates with hydrolysis of linuron to 3,4-dichloroaniline (dca) and n,o-dimethylhydroxylamine, followed by conversion of dca to krebs cycle intermediates. differential proteomic analysis showed a linuron-dependent upregulation of several enzymes that fit into this pathway, including an amidase (liba), a multicomponent chloroaniline dioxygenase, and enzymes associated with a ... | 2011 | 22003008 |
| lipopolysaccharide ip-pa1 from pantoea agglomerans prevents suppression of macrophage function in stress-induced diseases. | chronic psychological stress impairs health and induces various diseases by causing an imbalance in the immune, neuropsychiatric and endocrine systems. the primary reason for the development of stress-induced disease is suppression of macrophage function, which plays a pivotal role in innate immunity. in fact, surgical stress has been shown to exacerbate opportunistic infections by significantly suppressing macrophage function. conversely, administration of macrophage activating substances befor ... | 2011 | 21873156 |
| evaluation of microorganisms cultured from injured and repressed tissue regeneration sites in endangered giant aquatic ozark hellbender salamanders. | investigation into the causes underlying the rapid, global amphibian decline provides critical insight into the effects of changing ecosystems. hypothesized and confirmed links between amphibian declines, disease, and environmental changes are increasingly represented in published literature. however, there are few long-term amphibian studies that include data on population size, abnormality/injury rates, disease, and habitat variables to adequately assess changes through time. we cultured and i ... | 2011 | 22205979 |
| native microbiota shape insect vector competence for human pathogens. | the resident microbiota of insect vectors can impede transmission of human pathogens. recent studies have highlighted the capacity of endogenous bacteria to decrease viral and parasitic infections in mosquito and tsetse fly vectors by activating their immune responses or directly inhibiting pathogen development. these microbes may prove effective agents for manipulating the vector competence of malaria and other important human pathogens. | 2011 | 22018231 |
| Atomic resolution structure of EhpR: phenazine resistance in Enterobacter agglomerans Eh1087 follows principles of bleomycin/mitomycin C resistance in other bacteria. | The phenazines are redox-active secondary metabolites that a large number of bacterial strains produce and excrete into the environment. They possess antibiotic activity owing to the fact that they can reduce molecular oxygen to toxic reactive oxygen species. In order to take advantage of this activity, phenazine producers need to protect themselves against phenazine toxicity. Whereas it is believed that phenazine-producing pseudomonads possess highly active superoxide dismutases and catalases, ... | 2011 | 21849072 |
| efficient synthesis of hydroxystyrenes via biocatalytic decarboxylation/deacetylation of substituted cinnamic acids by newly isolated pantoea agglomerans strains. | background: decarboxylation of substituted cinnamic acids is a predominantly followed pathway for obtaining hydroxystyrenes-one of the most extensively explored bioactive compounds in the food and flavor industry (e.g. fema gras approved 4-vinylguaiacol). for this, mild and green strategies providing good yields with high product selectivity are needed. results: two newly isolated bacterial strains, i.e. pantoea agglomerans kjlpb4 and p. agglomerans kjpb2, are reported for mild and effective dec ... | 2011 | 21919002 |
| carbohydrate-active enzymes from pigmented bacilli: a genomic approach to assess carbohydrate utilization and degradation. | spore-forming bacilli are gram-positive bacteria commonly found in a variety of natural habitats, including soil, water and the gastro-intestinal (gi)-tract of animals. isolates of various bacillus species produce pigments, mostly carotenoids, with a putative protective role against uv irradiation and oxygen-reactive forms. | 2011 | 21892951 |
| Isolation of Bacteria with Antifungal Activity against the Phytopathogenic Fungi Stenocarpella maydis and Stenocarpella macrospora. | Stenocarpella maydis and Stenocarpella macrospora are the causal agents of ear rot in corn, which is one of the most destructive diseases in this crop worldwide. These fungi are important mycotoxin producers that cause different pathologies in farmed animals and represent an important risk for humans. In this work, 160 strains were isolated from soil of corn crops of which 10 showed antifungal activity against these phytopathogens, which, were identified as: Bacillus subtilis, Pseudomonas spp., ... | 2011 | 22016606 |
| Endospore production allows using spray-drying as a possible formulation system of the biocontrol agent Bacillus subtilis CPA-8. | The role of endospore production by Bacillus subtilis CPA-8 on survival during spray-drying was investigated by comparison with a non-spore-forming biocontrol agent Pantoea agglomerans CPA-2. Endospore formation promoted heat resistance in CPA-8 depending on growth time (72 h cultures were more resistant than 24 h ones). The survival of CPA-8 and CPA-2 after spray-drying was determined after being grown in optimised media for 24 and 72 h. Spray-dried 72 h CPA-8 had the best survival (32%), while ... | 2011 | 22187082 |