Publications

properties and roles of bacterial symbionts of polyvinyl alcohol-utilizing mixed cultures.	from several polyvinyl alcohol (pva)-utilizing mixed cultures, two component bacterial strains essential for pva utilization were isolated, and their properties and roles in pva utilization were studied. each pair of essential component strains consisted of a type i strain, which produced a pva-degrading enzyme and constituted the predominant population of the mixed culture in pva, and a type ii strain, which produced a certain growth stimulant for the former strain. all of the type i strains we ...	1983	6639015
[taxonomic study of free nitrogen-fixing bacteria isolated from the endorhizosphere of rice].	twenty strains of free-living n2-fixing bacteria, isolated from the endorhizosphere of rice in rice soils of senegal, were studied on the basis of 259 morphological, physiological, biochemical and nutritional characters. half of them were gram-negative small rods with polar flagella and showing a strictly respiratory metabolism; they were characteristic of the genus pseudomonas. a first group of 6 strains was related to the p. cepacia-p. marginata group characterized by lophotrichous flagella; t ...	1983	6651126
degradation of chlorophenols by a defined mixed microbial community.	synthetic sewage containing phenol, acetone, and alkanols plus 4-chlorophenol or a mixture of isomeric chlorophenols is completely degraded by a defined mixed culture with pseudomonas sp. strain b13 as a chlorocatechol-dissimilating member of the community. total degradation of the organic carbon was indicated by release of stoichiometric amounts of chloride and low content of dissolved organic carbon in the cell-free effluents. during adaptation to high loads of chlorophenols the initial meta-c ...	1983	6651293
studies on the use of a long-acting oxytetracycline in turkeys: efficacy against experimental infections with alcaligenes faecalis and pasteurella multocida.	a group of 2-week-old turkey poults was inoculated with a pathogenic strain of alcaligenes faecalis. one week later, half the group was injected with a long-acting oxytetracycline formulation. poults were examined post-mortem on days 3, 5, and 10 post-treatment. swabs were taken of the trachea, sinus, and lung of each poult. poults were observed closely for clinical signs, and appropriate tissues were taken for histopathology. results showed no evidence of therapeutic effect on a. faecalis infec ...	1983	6651700
in vitro cytotoxicity of an alcaligenes faecalis and its relationship in in vivo tracheal pathologic changes in turkeys.	two isolates of alcaligenes faecalis from turkeys with respiratory disease were indistinguishable physically, biochemically, and for specific agglutinating antibodies. the isolates differed in in vitro cytotoxicity for turkey tracheal organ cultures and in ability to induce clinical rhinotracheitis in poults. the isolate designated ncdp induced in vitro cytotoxic changes in turkey tracheal organ cultures. additionally, poults inoculated with ncdp developed severe clinical signs of rhinotracheiti ...	1983	6651702
presence of a new cytochrome b - like pigment with a peak at 567 nm in various aerobic bacteria.	several physiological groups of bacteria were examined for the presence of a cytochrome b - like pigment which is demonstrable in dithionite-reduced minus substrate-reduced difference spectra. this pigment is characterized by an unusually high alpha band at 567 nm, a low concentration relative to conventional cytochromes, and an inability to be fully reduced by endogenous substrates or nadh. previous studies with one denitrifying and nondenitrifying species of the genus pseudomonas, in paracoccu ...	1983	6652580
comparative study of antitumor activity and immunomodulatory effect of tetrahydro-2-furanyl and tetrahydro-2-pyranyl (1 leads to 3)-beta-d-glucans.	the effects of tetrahydro-2-furanyl- and tetrahydro-2-pyranyl-ethers of (1 leads to 3)-beta-d-glucans from alcaligenes faecalis var. myxogenes (ifo 13140) on the activities of macrophages and natural killer (nk) cells in either icr or balb/c mice were studied. the derivatives with strong antitumor activity against sarcoma 180 in icr mice induced higher macrophage tumoricidal activity (greater than 40%) than those with low antitumor activity (less than 20%). all derivatives with high or low antit ...	1983	6655547
alcaligenes fecalis septicemia.		1983	6680119
histamine-sensitizing factor of alcaligenes faecalis.	alcaligenes faecalis produced a histamine-sensitizing factor (hsf) in turkey poults and mice, which was detected in poults by an infraorbital sinus test and passive cutaneous anaphylaxis test and in mice by a rectal-temperature differential test. the a. faecalis hsf appeared to be similar to that produced by the genus bordetella and may be partly responsible for the clinical signs of alcaligenes rhinotracheitis in young poults.	1983	6682654
purification of a bacterial organophosphate-hydrolysing phosphatase by cibacron 3ga-sepharose affinity chromatography.	a phosphatase catalysing the hydrolysis of organophosphorus pesticides was purified to homogeneity using cibacron 3ga-sepharose cl 6b affinity chromatography. the enzyme which is localized in the periplasm of the bacterium alcaligenes nc5 was extracted by treating with 0.2m mgcl2, ph 8.4. the enzyme was adsorbed to the cibacron-sepharose at ph 7.0 and eluted with tris-hcl buffer at ph 8.0, with 47 per cent recovery. the enzyme thus obtained was electrophoretically homogeneous. this simple affini ...	1983	6301451
ph dependence of the reduction-oxidation reaction of azurin with cytochrome c-551: role of histidine-35 of azurin in electron transfer.	a fluorescence quenching experiment confirms that in the redox reaction between cytochrome c-551 and azurin, protein complexing is negligible. azurin-ph indicator t-jump experiments show that pseudomonas aeruginosa (ps.) azurin exhibits a slow time constant, tau, in its return to ph equilibrium but alcaligenes faecalis (alc.) azurin does not. the decrease of l/tau with increasing ph shows that the rate-determining process is a slow transformation of the imidazolium form of histidine-35 from a co ...	1983	6303402
purification and properties of the membrane-bound hydrogenase from n2-fixing alcaligenes latus.	the nitrogen-fixing, aerobic hydrogen-oxidizing bacterium alcaligenes latus forms hydrogenase when growing lithoautotrophically with hydrogen as electron donor and carbon dioxide as sole carbon source or when growing heterotrophically with n2 as sole nitrogen source. the hydrogenase is membrane-bound and relatively oxygen-sensitive. the enzymes formed under both conditions are identical on the basis of the following criteria: molecular mass, mobility in polyacrylamide gel electrophoresis, km val ...	1983	6305422
pathway of oxidation of pyruvic oxime by a heterotrophic nitrifier of the genus alcaligenes: evidence against hydrolysis to pyruvate and hydroxylamine.	a heterotrophic nitrifier of the genus alcaligenes, which grows vigorously on pyruvic oxime, was tested by several methods for possible differences or similarities in metabolic performance between pyruvic oxime and its hydrolysis products, pyruvate and hydroxylamine. major differences were observed between pyruvic oxime and one or both of the other reductants with regard to growth yield, rates of reductant uptake, rates of oxygen uptake, sensitivity of their oxidations to inhibition by thiocyana ...	1983	6307157
proton translocation during denitrification by a nitrifying--denitrifying alcaligenes sp.	a heterotrophic nitrifying alcaligenes sp. from soil was grown as a denitrifier on nitrate and subjected to oxidant pulse experiments to ascertain the apparent efficiencies of proton translocations during o2 and nitrogen-oxide respirations. with endogenous substrate as the reducing agent the leads to h+/2e- ratios, extrapolated to zero amount of oxidant per pulse, were 9.4, 3.7, 4.3 and 3.5 for o2, nitrate, nitrite and n2o, respectively. the value for o2 and those for the n-oxides are, respectiv ...	1983	6311094
susceptibility of 324 nonfermentative gram-negative rods to 6 cephalosporins and azthreonam.	susceptibility of 324 isolates of nonfermentative gram-negative bacteria to cephalothin, cefamandole, cefoxitin, ceftazidime, cefsulodin, and azthreonam was determined by agar dilution and disc diffusion techniques. with the exception of moraxella species, first- and second-generation cephalosporins were minimally active against nonfermenters tested. cefsulodin and azthreonam were mainly active against pseudomonas aeruginosa. in contrast, ceftazidime and ceftriaxone exhibited wider activity spec ...	1983	6311492
synergy between the iron chelator deferoxamine and the antimicrobial agents gentamicin, chloramphenicol, cefalothin, cefotiam and cefsulodin.	synergy between the iron chelator deferoxamine in the presence or absence of ascorbic acid and gentamicin, chloramphenicol, cephalothin, cefotiam or cefsulodin, used against staphylococcus aureus, staphylococcus epidermidis, escherichia coli, klebsiella pneumoniae, proteus mirabilis and species of salmonella, enterobacter, pseudomonas and providencia, was determined by measuring the effect of the drugs and combination of drugs on growth of the bacteria in an automated turbidimeter. the combinati ...	1983	6315421
comparative study of the beta-lactamase activity found in achromobacter.	a survey of 21 clinical isolates of achromobacter species demonstrated a high level of beta-lactamase activity in all strains tested. the beta-lactamases were characterized by isoelectric focusing, purification by affinity chromatography, determination of molecular weight, immunological identity, and genetic analysis. at least three distinct patterns of beta-lactamases were found in 19 strains. the kinetic values km and vmax measured by a microacidimetric method showed that all three types of en ...	1983	6193853
effect of various sources of organic carbon and high nitrite and nitrate concentrations on the selection of denitrifying bacteria. ii. continuous cultures in packed bed reactors.	the effect of different organic compounds, nitrites and nitrates at the concentration of 1,000 mg n/l on the quantitative and strain-specific selection of denitrifying bacteria was determined in anaerobic packed bed reactors. both the source of carbon and nitrogen form influenced strain specificity and the frequency of occurrence of denitrifying bacteria. the frequency of denitrifying bacteria within packed bed reactor ranged in different media from 11% (glucose and nitrates) to 100% (methanol a ...	1983	6194668
evolution of l-phenylalanine biosynthesis in rrna homology group i of pseudomonas.	group i pseudomonads exhibit diversity for l-phenylalanine biosynthesis that is a basis for separation of two subgroups. subgroup ib (fluorescent species such as pseudomonas aeruginosa, p. fluorescens, or p. putida) possesses an unregulated overflow pathway to l-phenylalanine, together with a second, regulated pathway. subgroup ia (non-fluorescent species such as p. stutzeri, p. mendocina, or p. alcaligenes) possess only the regulated pathway to l-phenylalanine. thus, subgroup ia species lack an ...	1983	6197946
a cleavage product of mouse c3 adsorbable to (1----3)-beta-d-glucan from alcaligenes faecalis var. myxogenes ifo 13140.	by using affinity chromatography of tak, a gel-forming antitumor (1----3)-beta-d-glucan produced by alcaligenes faecalis var. myxogenes ifo 13140 also known as an activator of the alternative complement pathway (acp), we obtained from mouse serum a cleavage product of c3 (tentatively designated as c3x). binding between c3x and tak seemed to be noncovalent. in contrast to c3, c3x had an intact beta chain (70,000 daltons) and instead of an alpha chain, two peptide chains (40,000 and 30,000 daltons ...	1983	6206261
regulation of hydrogenase formation is temperature sensitive and plasmid coded in alcaligenes eutrophus.	alcaligenes eutrophus grew well autotrophically with molecular hydrogen at 30 degrees c, but failed to grow at 37 degrees c (hox ts). at this temperature the strain grew well heterotrophically with a variety of organic compounds and with formate as an autotrophic substrate, restricting the thermolabile character to hydrogen metabolism. the soluble hydrogenase activity was stable at 37 degrees c. the catalytic properties of the wild-type enzyme were identical to those of a mutant able to grow lit ...	1983	6336732
[improvement of the quality control of thioglycol medium].	the effect of thimerosal used at different concentrations on the growth of s. haemolyticus, strain dick i, and a. faecalis, strain 415, has been studied. a. faecalis, strain 415, in contrast to s. haemolyticus, strain dick i, has been shown to be highly sensitive to thimerosal. the growth and neutralizing properties of a number of lots of thyoglycol medium have been studied with the use of the above-mentioned strains. the advantages of a. faecalis, strain 415, over s. haemolyticus, strain dick i ...	1983	6340394
purification of hydrogenases by affinity chromatography on procion red-agarose.	the agarose-coupled triazine dye procion red he-3b has been demonstrated to be applicable as an affinity gel for the purification of five diverse hydrogenases, namely the soluble, nad-specific and the membrane-bound hydrogenase of alcaligenes eutrophus, the membrane-bound hydrogenase of the n2-fixing alcaligenes latus, the reversible h2-evolving and the unidirectional h2-oxidizing hydrogenase of clostridium pasteurianum. in the case of the soluble hydrogenase of a. eutrophus, chromatography on p ...	1983	6351840
cultivation of the bacterial strain achromobacter delicatulus, producing an exocellular polyglucan-type polysaccharide in the fermentation tank fu-6.	growth processes and biosynthesis of the exocellular polyglucan-type polysaccharide, produced by the bacteria achromobacter delicatulus, were studied in the laboratory fermentation apparatus fu-6 under three completely different aeration systems. the purpose of this study was to find the most economical way of the polysaccharide biosynthesis. the growth rate and the synthesis of the exopolysaccharide were not limited either by the oxygen transfer or glucose content under the conditions examined. ...	1983	6359769
occurrence of deletion plasmids at high rates after conjugative transfer of the plasmids rp4 and rk2 from escherichia coli to alcaligenes eutrophus h16.	the broad host-range incp-1 plasmids rp4 and rk2 were transferred by conjugation from escherichia coli to alcaligenes eutrophus h16. among the transconjugants selected on media containing tetracycline, a considerable number did not express kanamycin resistance. by comparing restriction patterns of plasmids isolated from a large number of transconjugants a variety of different deletion derivatives were found. all of these possess more or less extended deletions always including parts of the tra 1 ...	1983	6360067
fermentation, isolation, characterization and structure of nitrosofungin.	the new antifungal agent nitrosofungin was isolated in high yields from a mixed culture of two organisms consisting of a bacterium of the genus alcaligenes (uc 9152) and streptomyces plicatus uc 8272. the bacterium produces the agent, the streptomycete enhances the production by providing a precursor or an inducer. nitrosofungin in high concentrations inhibits a broad variety of pathogenic fungi in vitro. the agent is relatively non-toxic in small laboratory animals and high blood levels are obt ...	1983	6360971
transfer and expression of klebsiella nif genes in alcaligenes faecalis, a nitrogen-fixing bacterium associated with rice root.	plasmid prd1 carrying klebsiella nif genes was found to be transferable by conjugation from escherichia coli jc5466 (prd1) to alcaligenes faecalis a-15 at a frequency of 5 x 10(-4). nitrogenase activity of four a-15 (prd1) strains tested was found to be higher than that of their parent a-15, as determined by the acetylene reduction assay. a-15-1 was a nif- mutant derived from a-15. after mating with jc5466 (prd1), the nitrogenase activity was restored. prd1 was stable in a. faecalis and could be ...	1983	6361825
production of curdlan-type polysaccharide by alcaligenes faecalis in batch and continuous culture.	the biosynthesis of a thermogelable, extracellular homo-beta-(1 leads to 3)-glucan called "curdlan," has been studied in batch and continuous cultures of alcaligenes faecalis var. myxogenes. curdlan production is associated with the poststationary phase of a nitrogen-depleted, aerobic batch culture. exopolymer is not detected in single-stage, carbon-limited continuous cultures but curdlan can be isolated from the effluent of a nitrogen-limited chemostat operating at a dilution rate (d) of less t ...	1983	6362809
effects of atmosphere of incubation on recovery of bacteria and yeasts from blood cultures in tryptic soy broth.	a comparison was made of the results of blood cultures between 1974 and 1981 in unvented and transiently vented bottles of tryptic soy broth under vacuum with co2. a total of 14,646 isolates were available for statistical analysis. significantly more isolates of staphylococcus epidermidis, staphylococcus aureus, bacillus, escherichia, pseudomonas, klebsiella, serratia, acinetobacter, alcaligenes, neisseria, and candida were recovered from the vented bottle. significantly more isolates of coryneb ...	1983	6370565
use of the api 20e, oxi/ferm, and minitek systems to identify nonfermentative and oxidase-positive fermentative bacteria: seven years of experience.	the api 20e (api) system, the oxi/ferm (oxf) system, and the minitek (min) system were used over a seven-year period to identify clinical isolates of nonfermentative bacteria (nfb) and oxidase-positive fermentative bacteria (opfb). a total of 742 nfb and opfb were tested with the api system, 988 with the oxf system, and 918 with the min system. the organisms represented 34 recognized species, nine alpha-numeric designates, and three species of unnamed nfb. results were compared to those obtained ...	1983	6370567
expression of hydrogenase in alcaligenes spp. is altered by interspecific plasmid exchange.	alcaligenes hydrogenophilus was found to contain a soluble and a particulate hydrogenase whose control and structure differed in part from that in alcaligenes eutrophus. one of at least two plasmids indigenous to a. hydrogenophilus determines hydrogenase genes (hox). the interspecific exchange of hox-encoding plasmids generated transconjugants which expressed the structural and regulatory hox phenotype of the donor.	1984	6370961
alcaligenes eutrophus hydrogenase genes (hox).	mutants of alcaligenes eutrophus h16 lacking catalytically active soluble hydrogenase (hos-) grew very slowly lithoautotrophically with hydrogen. mutants devoid of particulate hydrogenase activity (hop-) were not affected in growth with hydrogen. the use of hos- and hop- mutants as donors of hydrogen-oxidizing ability in crosses with plasmid-free recipients impaired in both hydrogenases (hox-) resulted in transconjugants which had inherited the plasmid and the phenotype of the donor. this indica ...	1984	6370965
evidence for isofunctional enzymes in the degradation of phenol, m- and p-toluate, and p-cresol via catechol meta-cleavage pathways in alcaligenes eutrophus.	a study of the degradation of phenol, p-cresol, and m- and p-toluate by alcaligenes eutrophus 345 has provided evidence that these compounds are metabolized via separate catechol meta-cleavage pathways. analysis of the enzymes synthesized by wild-type and mutant strains and by strains cured of the plasmid pra1000, which encodes m- and p-toluate degradation, indicated that two or more isofunctional enzymes mediated several steps in the pathway. the formation of three catechol 2,3-oxygenases and t ...	1984	6370966
regulation by molecular oxygen and organic substrates of hydrogenase synthesis in alcaligenes eutrophus.	chemoautotrophic growth of alcaligenes eutrophus 17707 is inhibited by 20% oxygen in the gas phase. lowering the oxygen concentration to 4% results in chloramphenicol-sensitive derepression of soluble and membrane-bound hydrogenase activity (and of soluble hydrogenase antigen), showing that oxygen inhibition is due at least in part to repression of hydrogenase synthesis. mutations resulting in derepression of hydrogenase activity (and antigen) under 25% oxygen (ose-) mobilized with a self-transm ...	1984	6376469
a multifunctional fermentative alcohol dehydrogenase from the strict aerobe alcaligenes eutrophus: purification and properties.	a nad (p)-linked alcohol dehydrogenase was isolated from the soluble extract of the strictly respiratory bacterium alcaligenes eutrophus n9a. derepression of the formation of this enzyme occurs only in cells incubated under conditions of restricted oxygen supply for prolonged times. the purification procedure included precipitation by cetyltrimethylammonium bromide and ammonium sulfate and subsequent chromatography on deae-sephacel, cibacron blue f3g-a sepharose and thiol-sepharose. the procedur ...	1984	6378632
hydrogen evolution by strictly aerobic hydrogen bacteria under anaerobic conditions.	when strains and mutants of the strictly aerobic hydrogen-oxidizing bacterium alcaligenes eutrophus are grown heterotrophically on gluconate or fructose and are subsequently exposed to anaerobic conditions in the presence of the organic substrates, molecular hydrogen is evolved. hydrogen evolution started immediately after the suspension was flushed with nitrogen, reached maximum rates of 70 to 100 mumol of h2 per h per g of protein, and continued with slowly decreasing rates for at least 18 h. ...	1984	6378884
evaluation of commercial test systems for the identification of nonfermenters.	three commercially available test systems for the identification of nonfermentative gram-negative rods were compared: api 20ne, flow n/f and minitek nonfermenter system. two hundred strains were identified by conventional means and by each test system. the rate of correct identification of acinetobacter, alcaligenes, flavobacterium and moraxella strains to genus level and of the other genera to species level was 92% with api 20ne, 84% with flow n/f and 75% with minitek nonfermenter system. the n ...	1984	6386458
[lysyl endopeptidase].		1984	6395195
evidence for adherence-dependent cytotoxicity of alcaligenes faecalis in turkey tracheal organ cultures.	a virulent isolate of alcaligenes faecalis was examined in turkey tracheal organ cultures (ttoc) for adherence using immunofluorescent staining and for cytotoxicity using light microscopic observation. treatment of the bacterial culture with trypsin, antiserum specific for a. faecalis, and n-acetylneuraminic acid inhibited the ability of the organism to adhere to ttoc. treatment of the bacterium with d-galactose partly decreased adherence of the bacterium. those treatments that inhibited the adh ...	1984	6395844
differentiation of gram negative rods other than enterobacteriaceae and vibrionaceae by a micromethod for determination of carbon substrate assimilation.	the assimilation techniques described for taxonomic study are unsuitable for routine diagnosis because of the need for purification and standardization of substrates, the great quantity of medium consumed and difficulties in interpreting the results. a standardized micromethod (api strip) for the study of carbon substrate assimilation by bacteria has recently been described (zbl. bakt. hyg., i. abt. orig. a 255 (1983) 479-488). the prototype gallery used consisted of two strips of 32 microtubes ...	1984	6397944
[a differential diagnostic scheme for identifying pigment-free variants of pseudomonas aeruginosa].		1984	6207378
chitynolytic bacteria in water and bottom sediments of two lakes of different trophy.	chitynolytic bacteria were found in greater numbers in the eutrophic lake jeziorak than in the mesotrophic lake jasne. in both lakes higher chitynolytic activity was found in the planktonic than in the benthic bacteria. the capacity of chitin hydrolysis was found in bacteria of the genera achromobacter, bacillus, nocardia, pseudomonas, of the flavobacterium-cytophaga group and of the enterobacteriaceae family. the chitinases synthesized by the studied bacteria were active in the ph range 5.0--8. ...	1984	6209933
partial characterization of the hemagglutinin of alcaligenes faecalis.	the hemagglutinin of alcaligenes faecalis was partially characterized. hemagglutination (ha) was blocked by enzymes inactivating proteins, by heat, and by antisera but not by sugar-blocking substances. pili were not determined to be a factor in ha activity. there was no connection between virulence and ha activity.	1984	6148928
bacterial microflora participating in the removal of nitrogen from industrial wastewaters by nitrification and denitrifaction.	nitrification and denitrification were used to purify industrial nitrogenous-organic wastewaters containing 1,130 mg n/l (mainly in the form of ammonium and urea) and with mean cod 1,050 mg o2/l. the use of a five-step system consisting of two denitrifying beds and three nitrifying activated sludge reactors gave 99% removal of nitrogen and 54% reduction in cod during total retention time of the wastes of 81 hours. the participation of stage i nitrifying bacteria in the activated sludge was 4.4 t ...	1984	6205553
control of autotrophic carbon assimilation in alcaligenes eutrophus by inactivation and reactivation of phosphoribulokinase.	phosphoribulokinase in alcaligenes eutrophus was partially inactivated when an autotrophic culture was shifted to heterotrophic growth with pyruvate as the sole source of carbon and energy. a similar response was observed on addition of various organic substrates to autotrophic cultures during the transition to mixotrophic growth. the extent of inactivation depended on the added substrate. pyruvate or lactate caused the strongest inactivation among the tested substrates. up to 75% of the phospho ...	1984	6317659
effect of nickel on activity and subunit composition of purified hydrogenase from nocardia opaca 1 b.	the nad-reducing hydrogenase of nocardia opaca 1 b was found to be a soluble, cytoplasmic enzyme. n. opaca 1 b does not contain an additional membrane-bound hydrogenase. the soluble enzyme was purified to homogeneity with a yield of 19% and a final specific activity of 45 mumol h2 oxidized min-1 mg protein-1. nad reduction with h2 was completely dependent on the presence of divalent metal ions (ni2+, co2+, mg2+, mn2+) or of high salt concentrations (0.5-1.5 m). the most specific effect was cause ...	1984	6319136
the identification and cloning of genes encoding haloaromatic catabolic enzymes and the construction of hybrid pathways for substrate mineralization.	this paper reviews the genetic basis of haloaromatic biodegradation by bacteria, with a focus on the genetic analysis of alcaligenes eutrophus jmp134, an organism which can utilize 3-chlorobenzoate, 2,4-dichlorophenoxyacetate (2,4-d) and related compounds as sole carbon and energy sources, and pseudomonas sp. b13, a chlorobenzoate degrader. the involvement of transmissible plasmids pjp4 and pwr1, isolated from strains jmp134 and b13, respectively, in chloroaromatic mineralization has been examin ...	1984	6322743
proton nmr spectroscopy of cytochrome c-554 from alcaligenes faecalis.	cytochrome c-554 from the bacterium alcaligenes faecalis (atcc 8750) is a respiratory electron-transport protein homologous to other members of the cytochrome c family. its structure has been studied by 1h nmr spectroscopy in both the ferric and ferrous states. the ferric spectrum is characterized by downfield hyperfine-shifted heme methyl resonances at 46.25, 43.60, 38.40, and 36.73 ppm (25 degrees c, ph 7.1). chemical shifts of these resonances change with temperature opposite to expectations ...	1984	6324856
characterization of a tol-like plasmid from alcaligenes eutrophus that controls expression of a chromosomally encoded p-cresol pathway.	alcaligenes eutrophus wild-type strain 345 metabolizes m- and p-toluate via a catechol meta-cleavage pathway. dna analysis, curing studies, and transfer of this phenotype by conjugation and transformation showed that the degradative genes are encoded on a self-transmissible 85-kilobase plasmid, pra1000. hindiii and xhoi restriction endonuclease analysis of pra1000 showed it to be similar to the archetypal tol plasmid, pwwo, differing in the case of hindiii only by the absence of fragments b and ...	1984	6325399
bacteremia caused by achromobacter species in an immunocompromised host.	a case of bacteremia caused by achromobacter species in an immunocompromised patient is described. the patient responded to antibiotic therapy. detailed antibiotic susceptibility data are presented.	1984	6332118
construction and use of a gene bank of alcaligenes eutrophus in the analysis of ribulose bisphosphate carboxylase genes.	a gene bank of the dna from the hydrogen bacterium alcaligenes eutrophus atcc 17707 was constructed in the broad host range cosmid vector pvk102 and established in escherichia coli. a triparental replica plating procedure was developed to allow rapid screening of large numbers of isolated e. coli gene bank clones for complementation of a. eutrophus mutants. this procedure was used to identify hybrid cosmids that complemented co2 fixation-negative (cfx-), h2 uptake-negative (hup-), and auxotrophi ...	1984	6090401
proton nmr spectroscopy of alcaligenes cytochrome c556.	a high molecular-weight c-type cytochrome was purified from alcaligenes faecalis atcc 8750. its weight was 40,000 daltons by sodium dodecyl sulfate-gel electrophoresis. heme content was determined to be one heme per 40,000 daltons. proton nuclear magnetic resonance-nmr-spectroscopy determined that the ferrous form is low spin. the detection of a methyl resonance at -3 ppm in the ferrous form indicated that methionine is a heme ligand in this state. the nmr spectrum of the ferric form at ph 7.2 r ...	1984	6092534
isolation and characterization of megaplasmid dna from lithoautotrophic bacteria.	a method is described for the preparative isolation of megaplasmids ranging in size from 340 to 700 kb. these plasmids were isolated from chemolithoautotrophic bacteria including the species alcaligenes, pseudomonas, and paracoccus. the procedure was based on alkaline sodium dodecyl sulfate lysis of the cells, followed by heat treatment, salt precipitation, several phenol extractions, dialysis steps, and proteinase and rnase treatment. the various parameters were evaluated and controlled. hydrog ...	1984	6098903
current views on the regulation of autotrophic carbon dioxide fixation via the calvin cycle in bacteria.	the calvin cycle of carbon dioxide fixation constitutes a biosynthetic pathway for the generation of (multi-carbon) intermediates of central metabolism from the one-carbon compound carbon dioxide. the product of this cycle can be used as a precursor for the synthesis of all components of cell material. autotrophic carbon dioxide fixation is energetically expensive and it is therefore not surprising that in the various groups of autotrophic bacteria the operation of the cycle is under strict meta ...	1984	6099093
a portable dna sequence carrying the cohesive site (cos) of bacteriophage lambda and the mob (mobilization) region of the broad-host-range plasmid rk2: a module for the construction of new cosmids.	a polylinker dna sequence carrying the cos site of bacteriophage lambda and the mob (orit) region of the incp group plasmid rk2 was constructed. this composite polylinker has ecori sites at both termini and also unique sites for clai, hindiii, psti and xbai. the cos-mob region is portable with the use of ecori or a combination of ecori with clai, hindiii or xbai. another cos-mob cassette was also constructed from which the cos-mob region can be lifted with hindiii, clai or either of these enzyme ...	1984	6099312
the pathology of an epizootic of acquired immunodeficiency in rhesus macaques.	a syndrome of acquired immunodeficiency within a group of outdoor-housed rhesus macaques (macaca mulatta) with unusually high mortality has been identified at the california primate research center. the cause of death for most of the affected animals included septicemia and/or chronic diarrhea with wasting, often complicated by other problems. in many cases, multiple or unusual infectious agents were isolated or recognized, including cytomegalovirus, cryptosporidium spp., and candida albicans. s ...	1984	6691418
phagocytosis of the legionnaires' disease bacterium (legionella pneumophila) occurs by a novel mechanism: engulfment within a pseudopod coil.	phagocytosis of legionella pneumophila, a bacterial pathogen that multiplies intracellularly in human mononuclear phagocytes and causes legionnaires' disease, occurs by a novel mechanism. a phagocyte pseudopod coils around the bacterium as the organism is internalized. human monocytes, alveolar macrophages, and polymorphonuclear leukocytes all phagocytize l. pneumophila by this unusual process, termed "coiling phagocytosis," and these leukocytes phagocytize not only live l. pneumophila in this w ...	1984	6692469
response of marine bacterioplankton to differential filtration and confinement.	the bacterioplankton community of confined seawater at 25 degrees c changed significantly within 16 h of collection. confinement increased cfu, total cell number (by epifluorescence microscopy), and average cell volume of bacterioplankton and increased the turnover rate of amino acids in seawater sampled at frying pan shoals, n.c. the bacterioplankton community was characterized by two components: differential doubling times during confinement shifted dominance from bacteria which were noncultur ...	1984	6696422
ornithine-containing lipids in thiobacillus a2 and achromobacter sp.	20 bacterial strains (corresponding to 16 species) were screened for ornithine lipids. only two species (thiobacillus a2 and achromobacter sp.) turned out to contain ornithine lipids (2.71 mmol/100 g and 0.38 mmol/100 g bacterial dry weight, respectively). in both ornithine lipids, a 3-hydroxy fatty acid was amide-linked to the alpha-amino group of ornithine, a normal fatty acid was ester-linked to the 3-hydroxy group of the former. the predominant fatty acids were 18:1(11) and 3-hydroxy-20:1(13 ...	1984	6698001
nosocomial colonization and infection by achromobacter xylosoxidans.	achromobacter xylosoxidans, a bacterial species named in 1971, is often isolated from aqueous environments, but little has been reported about its pathogenicity in humans, its epidemiological pattern, and its susceptibility to antibiotics and antiseptics. we were faced with an epidemic caused by this microorganism for 18 months in an intensive care unit. two patients had fatal infections and 37 others were colonized. the source was the deionized water of the hemodialysis system. the 46 isolates ...	1984	6699141
small angle x-ray study on the structure of active and inactive ribulose bisphosphate carboxylase from alcaligenes eutrophus. evidence for a configurational change.	two small angle x-ray scattering curves have been obtained from active and inactive ribulose 1,5-bisphosphate carboxylase from alcaligenes eutrophus. the radius of gyration was calculated to be r = 47.8 +/- 0.1 nm for the active enzyme and r = 49.2 +/- 0.1 nm for the inactive enzyme. the maximum particle dimension amounts to 13.5 +/- 0.5 nm for the active and 15.7 +/- 0.5 nm for the inactive enzyme. a model of the active carboxylase is presented. it is in good agreement with models derived from ...	1984	6707014
cleavage of formate from omega,4-dihydroxyacetophenone. an unusual oxygen-requiring reaction in the bacterial catabolism of 4-hydroxyacetophenone.	an enzyme, from a soil bacterium grown on 4-hydroxyacetophenone, cleaved the side chain of omega,4-dihydroxyacetophenone between the keto group and the carbon atom bearing a hydroxy group to give 4-hydroxybenzoate and formate. the reaction was o2-dependent. partially purified enzyme required no added cofactors for activity.	1984	6712612
differentiation of alcaligenes-like bacteria of avian origin and comparison with alcaligenes spp. reference strains.	although standard biochemical tests used for the identification of alcaligenes spp. revealed only minor differences, the oxidative low-peptone technique clearly differentiated between alcaligenes-like bacteria of avian origin and alcaligenes spp. reference strains. based on their colonial morphology, biochemical profiles, and hemagglutination, the alcaligenes-like bacteria of avian origin were further divided into two subgroups, c1-t1 and c2-t2. colonies of subgroup c1-t1 were nondescript, round ...	1984	6715517
heterotrophic nitrification among denitrifiers.	twelve denitrifying bacteria representing six genera were tested for an ability to nitrify pyruvic oxime heterotrophically. six of these bacteria exhibited appreciable nitrification activity, yielding as much as 5.8 mm nitrite and little or no nitrate when grown in a mineral salts medium containing 7 mm pyruvic oxime and 0.05% yeast extract. of the six active bacteria, four (pseudomonas denitrificans, pseudomonas aeruginosa, and two strains of pseudomonas fluorescens) could grow on yeast extract ...	1984	6721486
bactericidal activity of ethanol against glucose nonfermentative gram-negative bacilli.	the bactericidal effect of ethanol on glucose nonfermentative gram-negative bacilli (nonfermentative bacilli) and other species of micro-organisms was studied with emphasis on the former. at 20 degrees c, 10 to 20% v/v ethanol took 1 h or more to kill thirteen strains of nonfermentative bacilli while 40 to 99.5% concentrations produced a bactericidal effect within 1 min of exposure. eleven strains of glucose fermentative organisms showed a similar tendency to that noted with nonfermentative baci ...	1984	6727697
corneal ulcer due to achromobacter xylosoxidans.	we report a case of corneal ulcer caused by the opportunistic organism achromobacter xylosoxidans which developed during chronic topical steroid treatment of an eye with neovascular glaucoma. a. xylosoxidans has probably been underreported as a cause of ocular infection because of confusion between this organism and other gram-negative organisms, particularly pseudomonas. a. xylosoxidans is resistant to aminoglycosides and some cephalosporins but not carbenicillin. this difference in antibiotic ...	1984	6733072
microbial biodegradation of 4-chlorobiphenyl, a model compound of chlorinated biphenyls.	the biodegradation products of 4-chlorobiphenyl were analyzed in an achromobacter sp. strain and a bacillus brevis strain. both strains generated the same metabolites, with 4-chlorobenzoic acid as the major metabolic product. our results corroborate previous observations whereby most bacterial strains degrade the chlorobiphenyls via a major pathway which proceeds by an hydroxylation in position 2,3 and a meta-1,2 fission. however, we also detected several metabolites whose structure suggests the ...	1984	6742836
different local therapeutic effects of various polysaccharides on mh134 hepatoma in mice and its relation to inflammation induced by the polysaccharides.	the local antitumor activities and inflammation-inducing activities of various antitumor polysaccharides were examined and the relation between the two types of activity was studied. the tested antitumor polysaccharides included mg (a mannoglucan prepared from the culture fluid of microellobosporia grisea), lentinan, bacterial lipopolysaccharide, tak (a glucan from alcaligenes faecalis) and their derivatives. local antitumor activity was tested by intratumoral administration of the polysaccharid ...	1984	6745566
nmr spectroscopic identification of a hexacyanochromate(iii) binding site on pseudomonas azurin.	studies of redox reactions between the blue copper protein azurin and inorganic reagents have suggested the formation of discrete complexes between the reaction partners prior to the actual electron-transfer step. to get an insight into the structural nature of the complexes formed, we have studied the interaction (i) between oxidized pseudomonas aeruginosa azurin and the anion fe(cn)6(3-) and (ii) between reduced azurin and cr(cn)6(3-). at low ionic strengths, stoichiometric binding of one fe(c ...	1984	6426509
occurrence of s-adenosylhomocysteine hydrolase in prokaryote cells. characterization of the enzyme from alcaligenes faecalis and role of the enzyme in the activated methyl cycle.	the occurrence of s-adenosylhomocysteine hydrolase (ec 3.3.1.1) was found in a variety of prokaryotes. these prokaryotes did not exhibit any activities of s-adenosylhomocysteine nucleosidase (ec 3.2.2.9) and s-ribosyl-homocysteine hydrolase (ec 3.3.1.3), which had been the generally accepted prokaryote enzymes for the regeneration of free homocysteine from s-adenosylhomocysteine in the activated methyl cycle. in these prokaryotes s-adenosylhomocysteine hydrolase was suggested to be the only enzy ...	1984	6428887
unusual c3 and c4 metabolism in the chemoautotroph alcaligenes eutrophus.	phosphoenolpyruvate (pep) carboxykinase was identified to be the only c3-carboxylating enzyme in alcaligenes eutrophus. the enzyme requires gdp or inosine diphosphate (gtp or inosine triphosphate) for activity. pyruvate- and other pep-dependent co2-fixing enzyme activities were not detected, regardless of whether the cells were grown autotrophically or heterotrophically. it is suggested that two pathways are present in the organism for the formation of pep from c4 dicarboxylic acids. besides dec ...	1984	6429120
characterization of pseudomonads isolated from diseased fleece.	a total of 59 pseudomonas isolates was obtained from 11 samples of diseased fleece taken from live sheep. all but four of the isolates could be assigned to one of nine pseudomonas species, of which p. aeruginosa, p. alcaligenes, p. mendocina , and p. putida were the most common. p. aeruginosa was found in four of the fleece samples and, when present, appeared to predominate. although several of the isolates of p. aeruginosa lacked the ability to produce pyocyanin (and some produced neither pyocy ...	1984	6430232
[change in the cellular immune response under the influence of lipopolysaccharides].		1984	6443868
comparative cd-113 nuclear magnetic resonance studies of cd(ii)-substituted blue copper proteins.	the 113cd nmr spectra of plastocyanin (spinacea), stellacyanin (rhus vernicifera), and two azurins (pseudomonas aeruginosa and alcaligenes faecalis) have been measured after introducing cd(ii) into the blue copper-binding sites. relative to cd(c1o4)2 the chemical shifts are 432, 380, 372, and 379 ppm, respectively, all of which are found to be reasonable values for binding sites containing a cysteine thiolate ligand. the 113cd resonances of the cadmium derivatives of stellacyanin and the azurins ...	1984	6232270
[distribution of enterobacteria in soil following irrigation with waste water].	the filtration of wastewater after irrigation by sandy soil in the area of the braunschweig sewage utilization association was investigated. for that purpose, the concentrations of gram-negative aerobe rods were determined in soil samples of 15 cm, 30 cm, 45 cm, 60 cm, 75 cm, 90 cm, 105 cm, 120 cm, 135 cm, and 150 cm depth immediately after an irrigation of about 50 mm wastewater during the vegetation (3 series), the vegetationless period (6 series), after one week (3 series) and after two weeks ...	1984	6475379
evidence for plasmid dna in alcaligenes faecalis.	a 10.6 megadalton plasmid was isolated from a virulent strain (nc-d) of alcaligenes faecalis. virulence and antibiotic sensitivity of this strain were compared with those characteristics of a mutant plasmid-free derivative, strain nc-d1. strain nc-d1 was avirulent and lacked the streptomycin and sulfonamide resistances of the parent strain.	1984	6476581
immediate hypersensitivity (anaphylaxis) response in uninfected and alcaligenes faecalis-infected turkey poults.	the immediate hypersensitivity (anaphylaxis) response in uninfected and alcaligenes faecalis-infected turkey poults was studied. the optimum challenge dose of bovine serum albumin (bsa) to elicit an anaphylactic response was 40 mg/kg body weight, and the optimum poult age was 4 to 5 weeks. the response latency time was 7 days. the anaphylactic response was specific for the sensitizing antigen used. no differences were observed between a. faecalis-infected and uninfected poults in the immediate h ...	1984	6487189
evaluation of the rapid nft system for identification of gram-negative, nonfermenting rods.	this study evaluated the ability of the rapid nft system (api system sa, montalieu-vercieu, france) to accurately identify 262 clinically isolated, gram-negative, nonfermentative rods without additional tests. identifications were classified as correct; low discrimination, with a spectrum of two or more possibilities (additional tests necessary for accurate identification); and incorrect. correct identification rates were analyzed in two categories: (i) correct to species or biotype for all orga ...	1984	6490857
characterization of nitrite reductase from a denitrifier, alcaligenes sp. ncib 11015. a novel copper protein.	a copper-containing dissimilatory nitrite reductase [nitric-oxide: ferricytochrome c oxidoreductase, ec 1.7.2.1] was purified from a denitrifier, alcaligenes sp. ncib 11015, by ion-exchange chromatography on cm-cellulose, gel filtration on sephadex g-150, and adsorption on hydroxyapatite. the preparation was homogeneous by sds-polyacrylamide gel electrophoretic criteria, and its enzymatic activity increased considerably by freezing (at -20 degrees c) and thawing. the enzyme consists of two subun ...	1984	6501252
[toxicity of compounds formed after arsenic oxidation by microorganisms].		1984	6519479
alcaligenes faecalis rhinotracheitis in manitoba turkeys.	an outbreak of alcaligenes rhinotracheitis occurred on one premises housing five turkey flocks totaling 25,000 poults. prominent findings were severe respiratory difficulty resulting from excess mucus in the nasopharynx, lachrimation, and tracheal collapse. sinus and tracheal cultures consistently yielded alcaligenes faecalis. an adenovirus was isolated and four flocks became positive for celo virus by agar-gel-precipitin (agp) tests. mortality by flocks ranged from 4% to 48%. treatment was unsu ...	1984	6525132
in vitro cellular migration of leukocytes from turkey poults infected with alcaligenes faecalis.	the ability of peripheral blood leukocytes from young turkey poults to migrate in vitro was investigated. migration from capillary tubes was relatively rapid and was usually complete in 2 hours. leukocyte migration was significantly enhanced in alcaligenes faecalis-infected turkeys compared with uninfected controls at 1, 2, 5, and 6 weeks of age.	1984	6525134
prevalence of alcaligenes faecalis in north carolina broiler flocks and its relationship to respiratory disease.	in order to assess the role of alcaligenes faecalis in respiratory disease of broilers, a study was conducted to determine the prevalence of this bacterium in north carolina broilers and to determine the relationship of a. faecalis infection to clinical disease. our studies showed that a. faecalis is prevalent in north carolina commercial broilers during the winter months. bacteriological examination of turbinates and tracheas revealed that almost 40% of individual birds between 35 and 45 days o ...	1984	6525135
[sensitivity of non-fermenting gram-negative rods to antibiotics].		1984	6528605
[nitrogen metabolism in growing swine receiving a bacterial protein supplement (alcaligenes eutrophus) instead of soybean meal].	in a balance trial with 10 pigs (mean body mass 50 kg) the influence of a bacterial protein supplement (alcaligenes eutrophus) on n-metabolism was investigated. the bacteria were included into the diet at levels of 7 and 14% at the expense of extracted soyabean meal. thus bacterial "pure protein" (bacterial non-nucleic acid n x x 6.25) amounted to 30 and 60% of the protein of the ration. consuming 2 kg of feed dry matter per day the animals of the control group (i) and the experimental groups (i ...	1984	6539585
experimental studies on turkey coryza.	disease with severe respiratory symptoms, nasal discharge, swelling of sinuses, conjunctivitis with foamy discharge and mortality has been observed in turkey flocks in israel. of the pathogenic agents isolated from field cases, three, paramyxovirus, yucaipa (yucaipa virus), alcaligenes faecalis (a. faecalis) and mycoplasma gallisepticum (mg) were used to infect turkey poults, singly or in combination, in our attempt to reproduce the field disease symptoms. yucaipa virus alone caused mild symptom ...	1984	18766831
body temperature response of turkey poults infected with alcaligenes faecalis.	experiments were conducted to investigate the colonic temperature (tc) response to alcaligenes faecalis infection in turkeys. one-day-old poults were inoculated intranasally with 0.05 ml of a 24 hour brain heart infusion (bhi) broth culture with a bacterial concentration of 1.0 x 10(9) cells/ml. within 2 hours after inoculation tc was depressed significantly in challenged poults. this depression was significant through day 14 in experiment 1 and day 6 in experiment 2. further studies with steril ...	1984	18766888
enzymatic properties of immobilized alcaligenes faecalis cells with cell-associated beta-glucosidase activity.	enzymatic properties of alcaligenes faecalis cells immobilized in polyacrylamide were characterized and compared with those reported for the extracted enzyme, and with those measured for free cells. many of the properties reflected those of the extracted enzyme rather than those measured in the free whole cells prior to immobilization, suggesting cell disruption during immobilization. these properties included the ph activity profile, a slightly broader ph stability profile, and the activation e ...	1984	18553373
kinetics of growth of the hydrogen-oxidizing bacterium alcaligenes eutrophus (atcc 17707) in chemostat culture.	the hydrogen-oxidizing bacterium, alcaligenes eutrophus (atcc 17707), was grown in chemostat culture with gas-phase (hydrogen, oxygen, and carbon dioxide) and liquid-phase (mineral nutrients) feedstreams; data were used to generate an analytical form for the specific growth rate equation. model parameters obtained include monod rate parameters for dissolved hydrogen and oxygen gases, yield coefficients, and specific maintenance rates under conditions of hydrogen or oxygen limitations. these valu ...	1984	18553444
characterization of intracellular accumulation of poly-beta-hydroxybutyrate (phb) in individual cells of alcaligenes eutrophus h16 by flow cytometry.	poly-beta-hydroxybutyrate (phb) accumulates in individual cells of alcaligenes eutrophus in the form of refractile bodies which alter the light-scattering properties of individual cells. flow cytometry has been applied to measure the distributions of single-cell light-scattering intensity in alc. eutrophus populations during batch cultivation of the organism. these measurements clearly identify heterogeneities in the inoculum which influence the lag interval prior to beginning of exponential gro ...	1984	18553486
tropospheric sources of nox: lightning and biology.	laboratory experiments to quantify the global production of nox (no + no2) in the troposphere due to atmospheric lightning and biogenic activity in soil are presented. these laboratory experiments, as well as other studies, suggest that the global production of nox by lightning probably ranges between 2 and 20 mt(n)y-1 of no and is strongly dependent on the total energy deposited by lightning, a quantity not well-known. in our laboratory experiments, nitrifying micro-organisms is soil were fou ...	1984	11540827
degradation of the herbicide mecoprop [2-(2-methyl-4-chlorophenoxy)propionic acid] by a synergistic microbial community.	a microbial community isolated from wheat root systems was capable of growth on mecoprop as the sole carbon and energy source. when exposed to fresh herbicide additions, the community was able to shorten the lag phase from 30 days to less than 24 h. the community comprised two pseudomonas species, an alcaligenes species, a flavobacterium species, and acinetobacter calcoaceticus. none of the pure cultures was capable of growing on mecoprop. certain combinations of two or more community constituen ...	1985	16346731
effects of chemical speciation on the mineralization of organic compounds by microorganisms.	the mineralization of 1.0 to 100 ng each of four complexing compounds-oxalate, citrate, nitrilotriacetate (nta), and edta-per ml was tested in media prepared in accordance with equilibrium calculations by a computer program so that the h, ca, mg, fe, or al complex (chemical species) was predominant. sewage microorganisms mineralized calcium citrate more rapidly than iron, aluminum, or hydrogen citrate, and magnesium citrate was degraded slowest. aluminum, hydrogen, and iron oxalates were mineral ...	1985	16346854
the effect of combined and separate infection by alcaligenes faecalis and paramyxovirus (yucaipa) on the surface morphology of the trachea in turkey poults.	sixty-seven 1-day-old turkey poults were inoculated in the infra orbital sinus with 3 x 10(8) alcaligenes faecalis bacteria and/or by 10(5.8)eid 50/ ml of yucaipa virus. twenty-five similar birds served as controls. identification of the agents inoculated was made during the development of disease by means of isolation and serological tests. during the development of the disease, the surface morphology of the trachea in the affected animals revealed cellular oedema associated with a mucus coatin ...	1985	18766948
[the exocellular o-antigen polysaccharide of alcaligenes faecalis].		1985	2477670
quantitative selection of denitrifying bacteria in continuous cultures and requirement for organic carbon. i. starch.	a mixed population of bacteria from bottom sludge of nitrogen wastewater reservoir was incubated in continuous culture in medium containing 1000 mg nitrate nitrogen/l and starch. maximal efficiency of denitrification was 5 mg n/l/h. marked changes in participation of denitrifying bacteria (16-76%) among total number of bacteria was observed, this being dependent on the ratio of starch concentration (cs) to nitrogen concentration (cn) in the medium. the optimal cs/cn ratio ensuring highest partic ...	1985	2579531
nucleotide sequence, secondary structure and evolution of the 5s ribosomal rna from five bacterial species.	the nucleotide sequences of the 5s ribosomal rnas of the bacteria agrobacterium tumefaciens, alcaligenes faecalis, pseudomonas cepacia, aquaspirillum serpens and acinetobacter calcoaceticus have been determined. the sequences fit in a generally accepted model for 5s rna secondary structure. however, a closer comparative examination of these and other bacterial 5s rna primary structures reveals the potential of additional base pairing and of multiple equilibria between a set of slightly different ...	1985	2408888
[identification of pseudomonas and other similar gram-negative non-glucose-fermenting bacteria using a minimal number of tests].		1985	2409345
quantitative selection of denitrifying bacteria in continuous cultures and requirement for organic carbon. ii. maltose.	a mixed population of bacteria from industrial nitrogen wastewaters was incubated in continuous culture in medium containing 1000-1300 mg nitrate nitrogen per litre and maltose as a source of organic carbon. maximal efficiency of denitrification was 8.6 mg n/1/h. the participation of denitrifying bacteria in the culture varied from 0% to about 80%, depending on the ratio of maltose concentration (cm) to nitrogen concentration (cn) in the medium. the optimal cm/cn ratio ensuring the greatest sele ...	1985	2421546
prevention of bacterial contamination of water in dental units.	a study of the bacterial contamination of water in dental units was conducted in five dental practitioners' clinics and one university dental hospital in tokyo. various bacteria in numbers of 10(2)-10(5)/ml were detected in the water delivered from all 42 of the dental units examined, though very few bacteria were detected in the tap water upstream of the units. most of the organisms isolated from the water were gram-negative bacilli, predominantly alcaligenes, achromobacter and pseudomonas spp. ...	1985	2859327