Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| saliva: a diagnostic biomarker of periodontal diseases. | early detection of disease plays a crucial role in successful therapy. early diagnosis and management reduces the severity and possible complications of the disease process. to overcome this challenge, medical researchers are devoted to finding molecular disease biomarkers that reveal a hidden lethal threat before the disease becomes complicated. saliva, an important physiologic fluid, containing a highly complex mixture of substances, is rapidly gaining popularity as a diagnostic tool. periodon ... | 2011 | 22368352 |
| epithelial interleukin-8 responses to oral bacterial biofilms. | an in vitro model of bacterial biofilms on rigid gas-permeable contact lenses (rgpls) was developed to challenge oral epithelial cells. this novel model provided seminal data on oral biofilm-host cell interactions, and with selected bacteria, the biofilms were more effective than their planktonic counterparts at stimulating host cell responses. | 2011 | 21832101 |
| translational and clinical applications of salivary diagnostics. | there have been significant advances in techniques for the detection of biomarker signals in the oral cavity (e.g., elisas for proteins, pcr for rna and dna) as well as the engineering and development of microfluidic approaches to make oral-based point-of-care (poc) methods for the diagnosis for both local and systemic conditions a reality. in this section, we focus on three such approaches, namely, periodontal disease management, early markers for systemic diseases, and salivary markers useful ... | 2011 | 21917748 |
| ros-mediated signalling in bacteria: zinc-containing cys-x-x-cys redox centres and iron-based oxidative stress. | bacteria are permanently in contact with reactive oxygen species (ros), both over the course of their life cycle as well that present in their environment. these species cause damage to proteins, lipids, and nucleotides, negatively impacting the organism. to detect these ros molecules and to stimulate the expression of proteins involved in antioxidative stress response, bacteria use a number of different protein-based regulatory and sensory systems. ros-based stress detection mechanisms induce p ... | 2011 | 21977318 |
| ros-mediated signalling in bacteria: zinc-containing cys-x-x-cys redox centres and iron-based oxidative stress. | bacteria are permanently in contact with reactive oxygen species (ros), both over the course of their life cycle as well that present in their environment. these species cause damage to proteins, lipids, and nucleotides, negatively impacting the organism. to detect these ros molecules and to stimulate the expression of proteins involved in antioxidative stress response, bacteria use a number of different protein-based regulatory and sensory systems. ros-based stress detection mechanisms induce p ... | 2011 | 21977318 |
| inflammasomes in cardiovascular diseases. | nod-like receptors (nlrs) constitute a recently identified family of macromolecules that participate in regulation of innate immune responses. to date, 23 members of the nlr family are identified in humans. diverse nlrs are stimulated by a broad range of pathogen- or danger-associated molecular patterns, and collectively function as intracellular pattern recognition receptors (prrs). the most studied inflammasomes are nlrp1 and nlrp3 that process inactive pro-caspase-1 to its active form, allowi ... | 2011 | 22254202 |
| periodontal disease and tooth loss as risks for cancer: a systematic review of the literature. | periodontal disease is a chronic destructive disease which occurs in adults, young people, and children. periodontal disease and periodontal pathogens have been associated with several systemic diseases and more recently, several studies have suggested the relationship between periodontal disease and cancer. studies with adjustment for the effect of smoking exposure, have found significant positive associations with different cancer sites. this review has outlined recent epidemiologic researches ... | 2011 | 26322197 |
| bacterial interactions in dental biofilm. | biofilms are masses of microorganisms that bind to and multiply on a solid surface, typically with a fluid bathing the microbes. the microorganisms that are not attached but are free floating in an aqueous environment are termed planktonic cells. traditionally, microbiology research has addressed results from planktonic bacterial cells. however, many recent studies have indicated that biofilms are the preferred form of growth of most microbes and particularly those of a pathogenic nature. biofil ... | 2011 | 21778817 |
| papillon-lefévre syndrome. | the goal of periodontal therapy has always been regeneration of the lost tissues. however, conventional periodontal therapy has not always been successful in achieving regeneration, especially when it is part of a syndrome. this case report involves a 13-year old male patient with the chief complaint of mobile teeth for over 3 months. his dental history revealed early loss of primary dentition, around 3-4 years of age and that he noticed mobility of permanent incisors and molars at 9-10 years. k ... | 2011 | 22144846 |
| measurement of peroxiredoxin activity. | peroxiredoxins are cysteine-dependent peroxidases that react with hydrogen peroxide, larger hydroperoxide substrates, and peroxynitrite. protocols are provided to measure prx activity with peroxide by (1) a coupled reaction with nadph, thioredoxin reductase, and thioredoxin, (2) the direct monitoring of thioredoxin oxidation, (3) competition with horseradish peroxidase, and (4) peroxide consumption using the fox assay. | 2011 | 21818753 |
| benzyl isothiocyanate, a major component from the roots of salvadora persica is highly active against gram-negative bacteria. | plants produce a number of antimicrobial substances and the roots of the shrub salvadora persica have been demonstrated to possess antimicrobial activity. sticks from the roots of s. persica, miswak sticks, have been used for centuries as a traditional method of cleaning teeth. diverging reports on the chemical nature and antimicrobial repertoire of the chewing sticks from s. persica led us to explore its antibacterial properties against a panel of pathogenic or commensal bacteria and to identif ... | 2011 | 21829688 |
| phenytoin-induced gingival overgrowth: a review of the molecular, immune, and inflammatory features. | gingival overgrowth (go) is a side effect associated with some distinct classes of drugs, such as anticonvulsants, immunosuppressant, and calcium channel blockers. go is characterized by the accumulation of extracellular matrix in gingival connective tissues, particularly collagenous components, with varying degrees of inflammation. one of the main drugs associated with go is the antiepileptic phenytoin, which affects gingival tissues by altering extracellular matrix metabolism. nevertheless, th ... | 2011 | 21991476 |
| evaluation of two mouth rinses in reduction of oral malodor using a spectrophotometric technique. | various mouth rinses have been used in the treatment of halitosis, but most of the evidence for the efficacy of mouth rinses is anecdotal. in the present study, 0.2% chlorhexidine rinse and an essential oil mouth rinse are compared for their efficacy in reducing the breath mercaptan levels. | 2011 | 22028512 |
| gingipains from porphyromonas gingivalis - complex domain structures confer diverse functions. | gingipains, a group of arginine or lysine specific cysteine proteinases (also known as rgpa, rgpb and kgp), have been recognized as major virulence factors in porphyromonas gingivalis. this bacterium is one of a handful of pathogens that cause chronic periodontitis. gingipains are involved in adherence to and colonization of epithelial cells, haemagglutination and haemolysis of erythrocytes, disruption and manipulation of the inflammatory response, and the degradation of host proteins and tissue ... | 2011 | 24466435 |
| critical analysis of biomarkers in the current periodontal practice. | periodontal disease is a chronic microbial infection that triggers inflammation-mediated loss of the periodontal ligament and alveolar bone that supports the teeth. because of the increasing prevalence and associated comorbidities, there is a need for the development of new diagnostic tests that can detect the presence of active disease, predict future disease progression, and evaluate the response to periodontal therapy, thereby improving the clinical management of periodontal patients. the dia ... | 2011 | 21976831 |
| genomes of the most dangerous epidemic bacteria have a virulence repertoire characterized by fewer genes but more toxin-antitoxin modules. | we conducted a comparative genomic study based on a neutral approach to identify genome specificities associated with the virulence capacity of pathogenic bacteria. we also determined whether virulence is dictated by rules, or if it is the result of individual evolutionary histories. we systematically compared the genomes of the 12 most dangerous pandemic bacteria for humans ("bad bugs") to their closest non-epidemic related species ("controls"). | 2011 | 21437250 |
| pathologic crossroads: cardio-vascular diseases, periodontal diseases and calcium antagonists. | during the last decennium a more focused attention has been directed to the presence of chronic inflammation in cardiovascular diseases (cvd), but mainly to the high impact that this one has in generating and fastening the atherosclerotic process. | 2011 | 21505569 |
| periodontal treatment effects on endothelial function and cardiovascular disease biomarkers in subjects with chronic periodontitis: protocol for a randomized clinical trial. | periodontal disease (pd) is an infectious clinical entity characterized by the destruction of supporting tissues of the teeth as the result of a chronic inflammatory response in a susceptible host. it has been proposed that pd as subclinical infection may contribute to the etiology and to the pathogenesis of several systemic diseases including atherosclerosis. a number of epidemiological studies link periodontal disease/edentulism as independent risk factor for acute myocardial infarction, perip ... | 2011 | 21324167 |
| microbial shift and periodontitis. | 2011 | 21134227 | |
| evaluating the stoichiometry of macromolecular complexes using multisignal sedimentation velocity. | gleaning information regarding the molecular physiology of macromolecular complexes requires knowledge of their component stoichiometries. in this work, a relatively new means of analyzing sedimentation velocity (sv) data from the analytical ultracentrifuge is examined in detail. the method depends on collecting concentration profile data simultaneously using multiple signals, like rayleigh interferometry and uv spectrophotometry. if the cosedimenting components of a complex are spectrally disti ... | 2011 | 21256217 |
| defining pathogenic bacterial species in the genomic era. | actual definitions of bacterial species are limited due to the current criteria of definition and the use of restrictive genetic tools. the 16s ribosomal rna sequence, for example, has been widely used as a marker for phylogenetic analyses; however, its use often leads to misleading species definitions. according to the first genetic studies, removing a certain number of genes from pathogenic bacteria removes their capacity to infect hosts. however, more recent studies have demonstrated that the ... | 2011 | 21687765 |
| defining pathogenic bacterial species in the genomic era. | actual definitions of bacterial species are limited due to the current criteria of definition and the use of restrictive genetic tools. the 16s ribosomal rna sequence, for example, has been widely used as a marker for phylogenetic analyses; however, its use often leads to misleading species definitions. according to the first genetic studies, removing a certain number of genes from pathogenic bacteria removes their capacity to infect hosts. however, more recent studies have demonstrated that the ... | 2011 | 21687765 |
| changes in the subgingival biofilm composition after coronally positioned flap. | this study evaluated the effects of coronally positioned flap (cpf) on the subgingival biofilm composition. | 2011 | 21437473 |
| biofilm: a dental microbial infection. | recent advances in research technology have allowed researchers to study bacteria in their natural environment. dental biofilm forms via an ordered sequence of events, resulting in structured and functionally organized species rich microbial community and modern molecular biological techniques have identified about 1000 different bacterial species in the dental biofilm, twice as many as can be cultured. sites for biofilm formation include all kinds of surfaces: natural materials above and below ... | 2011 | 22470238 |
| pulpal perio relations: interdisciplinary diagnostic approach - i. | lesions of pulpal and periodontal origin may perpetuate from either the infections of dental pulp or periodontium or alveolar bone. this review focuses on interdisciplinary diagnostic approach towards lesions of periodontal or endodontic origin. | 2011 | 21772729 |
| probiotics in periodontal health and disease. | macfarlane burnett stated in 1962 that "by the late twentieth century, we can anticipate the virtual elimination of infectious diseases as a significant factor in social life". probiotics have become of interest to researchers in recent times. time has come to shift the paradigm of treatment from specific bacteria elimination to altering bacterial ecology by probiotics. the development of resistance to a range of antibiotics by some important pathogens has raised the possibility of a return to t ... | 2011 | 21772717 |
| saliva as a diagnostic fluid. | salivary diagnostics is a dynamic and emerging field utilizing nanotechnology and molecular diagnostics to aid in the diagnosis of oral and systemic diseases. in this article the author critically reviews the latest advances using oral biomarkers for disease detection. the use of oral fluids is broadening perspectives in clinical diagnosis, disease monitoring, and decision making for patient care. important elements determining the future possibilities and challenges in this field are also discu ... | 2011 | 21094724 |
| filament formation associated with spirochetal infection: a comparative approach to morgellons disease. | bovine digital dermatitis is an emerging infectious disease that causes lameness, decreased milk production, and weight loss in livestock. proliferative stages of bovine digital dermatitis demonstrate keratin filament formation in skin above the hooves in affected animals. the multifactorial etiology of digital dermatitis is not well understood, but spirochetes and other coinfecting microorganisms have been implicated in the pathogenesis of this veterinary illness. morgellons disease is an emerg ... | 2011 | 22253541 |
| molecular evolution of urea amidolyase and urea carboxylase in fungi. | urea amidolyase breaks down urea into ammonia and carbon dioxide in a two-step process, while another enzyme, urease, does this in a one step-process. urea amidolyase has been found only in some fungal species among eukaryotes. it contains two major domains: the amidase and urea carboxylase domains. a shorter form of urea amidolyase is known as urea carboxylase and has no amidase domain. eukaryotic urea carboxylase has been found only in several fungal species and green algae. in order to elucid ... | 2011 | 21447149 |
| identification of surprisingly diverse type iv pili, across a broad range of gram-positive bacteria. | in gram-negative bacteria, type iv pili (tfp) have long been known to play important roles in such diverse biological phenomena as surface adhesion, motility, and dna transfer, with significant consequences for pathogenicity. more recently it became apparent that gram-positive bacteria also express type iv pili; however, little is known about the diversity and abundance of these structures in gram-positives. computational tools for automated identification of type iv pilins are not currently ava ... | 2011 | 22216142 |
| annotation of protein domains reveals remarkable conservation in the functional make up of proteomes across superkingdoms. | the functional repertoire of a cell is largely embodied in its proteome, the collection of proteins encoded in the genome of an organism. the molecular functions of proteins are the direct consequence of their structure and structure can be inferred from sequence using hidden markov models of structural recognition. here we analyze the functional annotation of protein domain structures in almost a thousand sequenced genomes, exploring the functional and structural diversity of proteomes. we find ... | 2011 | 24710297 |
| variations of oral microbiota are associated with pancreatic diseases including pancreatic cancer. | objectivethe associations between oral diseases and increased risk of pancreatic cancer have been reported in several prospective cohort studies. in this study, we measured variations of salivary microbiota and evaluated their potential associations with pancreatic cancer and chronic pancreatitis.methodsthis study was divided into three phases: (1) microbial profiling using the human oral microbe identification microarray to investigate salivary microbiota variation between 10 resectable patient ... | 2011 | 21994333 |
| variations of oral microbiota are associated with pancreatic diseases including pancreatic cancer. | objectivethe associations between oral diseases and increased risk of pancreatic cancer have been reported in several prospective cohort studies. in this study, we measured variations of salivary microbiota and evaluated their potential associations with pancreatic cancer and chronic pancreatitis.methodsthis study was divided into three phases: (1) microbial profiling using the human oral microbe identification microarray to investigate salivary microbiota variation between 10 resectable patient ... | 2011 | 21994333 |
| Treatment of residual pockets with photodynamic therapy, diode laser, or deep scaling. A randomized, split-mouth controlled clinical trial. | The objective of this work was to compare the effects of antimicrobial photodynamic therapy (PDT), diode soft laser therapy (DSL), and thorough deep scaling and root planing (SRP) for treatment of residual pockets. Thirty-two subjects with a history of non-surgical treatment for chronic periodontitis were included. Residual pockets >4 mm and bleeding upon probing were debrided with an ultrasonic device and then subjected to either PDT, DSL, or SRP. Pocket probing depth (PPD), bleeding on probing ... | 2011 | 22105837 |
| human microbiome and hiv/aids. | understanding of the human microbiome continues to grow rapidly; however, reports on changes in the microbiome after hiv infection are still limited. this review surveys the progress made in methodology associated with microbiome studies and highlights the remaining challenges to this field. studies have shown that commensal oral, gut, vaginal, and penile bacteria are vital to the health of the human immune system. our studies on crosstalk among oral and gastrointestinal soluble innate factors, ... | 2011 | 22193889 |
| A New Real-Time PCR Assay for Improved Detection of the Parasite Babesia microti. | Babesiosis is an emerging zoonosis with important public health implications, as the incidence of the disease has risen dramatically over the past decade. Because the current gold standard for detection of Babesia is microscopic examination of blood smears, accurate identification requires trained personnel. Species in the genus cannot be distinguished microscopically, and Babesia can also be confused with the early trophozoite stage (ring forms) of Plasmodium parasites. To allow more accurate d ... | 2011 | 22170915 |
| colonisation of the oral cavity by periodontopathic bacteria in complete denture wearers. | gerodontology 2011; doi: 10.1111/j.1741-2358.2011.00506.x colonisation of the oral cavity by periodontopathic bacteria in complete denture wearers objective: the purpose of this study was to investigate colonisation by periodontopathic bacteria and the sites of colonisation in elderly upper and lower complete denture wearers. we also investigated the relationship between level of oral hygiene and colonisation by periodontopathic bacteria. materials and methods: forty edentulous and 37 dentate ... | 2011 | 21929616 |
| life cycle stage-resolved proteomic analysis of the excretome/secretome from strongyloides ratti--identification of stage-specific proteases. | a wide range of biomolecules, including proteins, are excreted and secreted from helminths and contribute to the parasite's successful establishment, survival, and reproduction in an adverse habitat. excretory and secretory proteins (esp) are active at the interface between parasite and host and comprise potential targets for intervention. the intestinal nematode strongyloides spp. exhibits an exceptional developmental plasticity in its life cycle characterized by parasitic and free-living gener ... | 2011 | 21964353 |
| Comparative analysis of salivary bacterial microbiome diversity in edentulous infants and their mothers or primary care givers using pyrosequencing. | Bacterial contribution to oral disease has been studied in young children, but there is a lack of data addressing the developmental perspective in edentulous infants. Our primary objectives were to use pyrosequencing to phylogenetically characterize the salivary bacterial microbiome of edentulous infants and to make comparisons against their mothers. Saliva samples were collected from 5 edentulous infants (mean age?=?4.6±1.2 mo old) and their mothers or primary care givers (mean age?=?30.8±9.5 y ... | 2011 | 21853142 |
| the capsule of porphyromonas gingivalis leads to a reduction in the host inflammatory response, evasion of phagocytosis, and increase in virulence. | periodontal disease is a chronic oral inflammatory disease that is triggered by bacteria such as porphyromonas gingivalis. p. gingivalis strains exhibit great heterogeneity, with some strains being encapsulated while others are nonencapsulated. although the encapsulated strains have been shown to be more virulent in a mouse abscess model, so far the role of the capsule in p. gingivalis interactions with host cells is not well understood and its role in virulence has not been defined. here, we in ... | 2011 | 21911459 |
| molecular detection of human periodontal pathogens in oral swab specimens from dogs in japan. | periodontal diseases are known to be major diseases in humans, and are also common in dogs. the purpose of the present study was to analyze the distribution of periodontitis-related bacterial species using oral swab specimens collected from 26 pet dogs. the distribution of an animal gingival organism porphyromonas gulae, in addition to 10 human periodontitis-related bacterial species, including porphyromonas gingivalis, treponema denticola, tannerella forsythia, capnocytophaga ochracea, capnocyt ... | 2011 | 21916371 |
| probiotics and periodontal health. | periodontitis is one of the most common chronic inflammatory diseases. the etiology is clearly bacterial and a number of putative bacterial pathogens have been associated with the disease, including aggregatibacter actinomycetemcomitans, tannerella forsythus and porphyromonas gingivalis. comparatively, little attention has been paid to the identification of health-associated and potentially beneficial bacterial species that may reside in the gingival sulcus. probiotic technology represents a bre ... | 2011 | 22514571 |
| relationship between the pathogenic representatives of periodontal pockets microbiocenosis in patients with periodontitis with varying degrees of severity. | periodontitis is a common disease that is considered to be a manifestation of the distortion of the ratio between the normal and conditionally pathogenic microflora of periodontal pockets. in this study, the ratio between the six most important periodontal pathogens and the total microflora of the periodontal pocket in healthy individuals and patients with varying severity of periodontitis was ascertained by quantitative real-time pcr. it was ascertained that the relative content ofporphyromonas ... | 2011 | 22649688 |
| comparison of real-time polymerase chain reaction and dna-strip technology in microbiological evaluation of periodontitis treatment. | the impact of a semiquantitative commercially available test based on dna-strip technology (microident®, hain lifescience, nehren, germany) on diagnosis and treatment of severe chronic periodontitis of 25 periodontitis patients was evaluated in comparison with a quantitative in-house real-time pcr. subgingival plaque samples were collected at baseline as well as at 3, 6, and 12 months later. after extracting dna, aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, tannerella forsyth ... | 2011 | 21146709 |
| a prospective cohort study of periodontal disease measures and cardiovascular disease markers in hiv-infected adults. | abstract the determinants of hiv-associated cardiovascular disease (cvd) are not well understood. periodontal disease (pd) has been linked to cvd but this connection has not been examined in hiv infection. we followed a cohort of hiv-infected adults to ascertain whether pd was associated with carotid artery intima media thickness (imt) and brachial artery flow-mediated dilation (fmd). we performed a longitudinal observational study of hiv-infected adults on haart for <2 years with no known heart ... | 2011 | 21443451 |
| molecular signaling mechanisms of the periopathogen, treponema denticola. | in the healthy subgingiva, oral treponemes account for a small percentage of the total bacteria. however, in diseased periodontal pockets, treponemes thrive and become a dominant component of the bacterial population. oral treponemes are uniquely adept at capitalizing on the environmental conditions that develop with periodontal disease. the molecular basis of adaptive responses of oral treponemes is just beginning to be investigated and defined. the completion of several treponeme genome sequen ... | 2011 | 21447698 |
| comparison of real-time pcr assays for detection of pathogenic leptospira spp. in blood and identification of variations in target sequences. | leptospirosis is considered an underdiagnosed disease. although several pcr-based methods are currently in use, there is little information on their comparability. in this study, four quantitative real-time pcr (qpcr) assays (sybr green and taqman chemistries) targeting the secy, lfb1, and lipl32 genes were evaluated as diagnostic assays. in our hands, these assays can detect between 10(2) and 10(3) bacteria/ml of pure culture, whole-blood, plasma, and serum samples. in three independent experim ... | 2011 | 21471336 |
| oral health behaviors, periodontal disease and pathogens in preeclampsia: a case-control study in korea. | background: the aim of this study was to confirm the association among oral health behaviors, periodontitis and preeclampsia in korean women. methods: this study was designed as a case-control study. sixteen preeclamptic and forty eight non-preeclamptic women after delivery were included in this study from november 2007 to january 2010. information on demographics, health behaviors, obstetric and systemic disease that may influence periodontal condition and preeclampsia was collected. full mouth ... | 2011 | 21486182 |
| microbial shifts during dental biofilm re-development in the absence of oral hygiene in periodontal health and disease. | uzel ng, teles fr, teles rp, song xq, torresyap g, socransky ss, haffajee ad. microbial shifts during dental biofilm re-development in the absence of oral hygiene in periodontal health and disease. j clin peridontol 2011; doi: 10.1111/j.1600-051x.2011.01730.x. abstract: aim: to monitor microbial shifts during dental biofilm re-development. materials and methods: supra- and subgingival plaque samples were taken separately from 28 teeth in 38 healthy and 17 periodontitis subjects at baseline and i ... | 2011 | 21488936 |
| a novel approach in the use of subgingival controlled-release delivery of chlorhexidine in chronic periodontitis: a randomized clinical trial. | background: we aimed to analyze clinical, microbiological, and serological effects of chlorhexidine (chx) chips used as subgingival controlled-release-delivery-device before and immediately after scaling and root-planing (srp). methods: 24 patients presenting with at least 12 teeth with ppd ≥5 mm and bop were assigned in test or control groups. after prophylaxis, chx chips (test) or placebo chips (control) were placed in pockets with ppd ≥5mm. ten days later srp was performed in all teeth with p ... | 2011 | 21491990 |
| gingival tissue transcriptomes in experimental gingivitis. | jönsson d, ramberg p, demmer rt, kebschull m, dahlén g, papapanou pn. gingival tissue transcriptomes in experimental gingivitis. j clin periodontol 2011; doi: 10.1111/j.1600-051x.2011.01719.x. abstract: aims: we investigated the sequential gene expression in the gingiva during the induction and resolution of experimental gingivitis. material and methods: twenty periodontally and systemically healthy non-smoking volunteers participated in a 3-week experimental gingivitis protocol, followed by deb ... | 2011 | 21501207 |
| viral-bacterial associations in acute apical abscesses. | objective: viral-bacterial and bacterial synergism have been suggested to contribute to the pathogenesis of several human diseases. this study sought to investigate the possible associations between 9 candidate endodontic bacterial pathogens and 9 human viruses in samples from acute apical abscesses. study design: dna extracts from purulent exudate aspirates of 33 cases of acute apical abscess were surveyed for the presence of 9 selected bacterial species using a 16s ribosomal rna gene-based nes ... | 2011 | 21507684 |
| inhibition of host- and bacteria-derived proteinases by natural anthocyanins. | santos j, la vd, bergeron c, grenier d. inhibition of host- and bacteria-derived proteinases by natural anthocyanins. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01372.x. © 2011 john wiley & sons a/s background and objectives: host- and bacteria-derived proteinases are considered to play critical roles in periodontitis progression. this study investigated the ability of a blackcurrant extract and its major anthocyanins (cyanidin-3-o-glucoside, cyanidin-3-o-rutinoside and delphinidin-3-o ... | 2011 | 21517858 |
| s100a2 level changes are related to human periodontitis. | periodontitis is an inflammatory disease, which, when severe, can result in tooth loss, that affects the quality of life. s100a2 was previously identified as a component of gingival crevicular fluid (gcf) via proteome analysis, but it has not been investigated whether s100a2 plays a role in periodontitis. in this study, we analyzed mrna expression of s100a2 in gingival tissues from normal and classified periodontal disease patients and compared it to that of s100a8 and s100a9. quantitative real ... | 2011 | 21922197 |
| Early microbial succession in redeveloping dental biofilms in periodontal health and disease. | Teles FR, Teles RP, Uzel NG, Song XQ, Torresyap G, Socransky SS, Haffajee AD. Early microbial succession in redeveloping dental biofilms in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01409.x. © 2011 John Wiley & Sons A/S Background and Objective: The development of dental biofilms after professional plaque removal is very rapid. However, it is not clear whether most bacterial species return at similar rates in periodontally healthy and periodontitis subj ... | 2011 | 21895662 |
| Early microbial succession in redeveloping dental biofilms in periodontal health and disease. | Teles FR, Teles RP, Uzel NG, Song XQ, Torresyap G, Socransky SS, Haffajee AD. Early microbial succession in redeveloping dental biofilms in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01409.x. © 2011 John Wiley & Sons A/S Background and Objective: The development of dental biofilms after professional plaque removal is very rapid. However, it is not clear whether most bacterial species return at similar rates in periodontally healthy and periodontitis subj ... | 2011 | 21895662 |
| composition and localization of treponema denticola outer membrane complexes. | the treponema denticola outer membrane lipoprotein-protease complex (dentilisin) contributes to periodontal disease by degrading extracellular matrix components and disrupting intercellular host signaling pathways. we recently demonstrated that prcb, located upstream of and cotranscribed with prca and prtp, encodes a 22-kda lipoprotein that interacts with prtp and is required for its activity. here we further characterize products of the protease locus and their roles in expression, formation, a ... | 2011 | 21986628 |
| [Role of several periodontopathogenic microorganisms and tlr4 gene Asp299Gly polymorphism in atherosclerosis pathogenesis]. | Establishment of presence of periodonto-pathogenic microorganisms in atherosclerosis plaque and surrounding tissues, and possible relation of development of atherosclerosis and TLR4 gene Asp299Gly polymorphism in ischemic heart disease patients (IHD). | 2011 | 22145357 |
| evidence for graft colonization with periodontal pathogens in lung transplant recipients. | summary bronchiolitis obliterans syndrome (bos) is a major cause of late graft dysfunction in lung transplant recipients. there is increasing evidence that beside alloimmunologic injury also non-alloimmunologic inflammatory conditions may raise the risk of acute and chronic rejection. the oral cavity represents a possible reservoir for pathogenic bacteria due to its close anatomical proximity. in this pilot study, the presence of pathogenic periodontal bacteria in the oral cavity as well as in t ... | 2011 | 22203528 |
| correlation network analysis applied to complex biofilm communities. | the complexity of the human microbiome makes it difficult to reveal organizational principles of the community and even more challenging to generate testable hypotheses. it has been suggested that in the gut microbiome species such as bacteroides thetaiotaomicron are keystone in maintaining the stability and functional adaptability of the microbial community. in this study, we investigate the interspecies associations in a complex microbial biofilm applying systems biology principles. using corr ... | 2011 | 22163302 |
| the relationship of the oral microbiotia to periodontal health and disease. | the oral microbial community represents the best-characterized consortium associated with the human host. there are strong correlations between the qualitative composition of the oral microbiota and clinically healthy or diseased states. however, additional studies are needed to elucidate the mechanisms that define these microbial/host relationships. | 2011 | 22018230 |
| detection of periodontal pathogens in newborns and children with mixed dentition. | we report the age-related prevalence of red complex periodontal pathogens, porphyromonas gingivalis, treponema denticola, and tannerella forsythia, along with four strains of orange complex pathogens. the bacteria present in samples isolated from tongue, cheek, and subgingival sulcus in edentulous newborns and children with mixed dentition were monitored by polymerase chain reaction (pcr). p. gingivalis was not detected in any site of any subject in the two groups tested. however, t. denticola w ... | 2011 | 21928086 |
| a machine-learning approach to identify hydrogenosomal proteins in trichomonas vaginalis. | the protozoan parasite trichomonas vaginalis is the causative agent of trichomoniasis, the most widespread non-viral sexually transmitted disease in humans. it possesses hydrogenosomes - anaerobic mitochondria that generate h(2), co(2), and acetate from pyruvate while converting adp to atp via substrate level phosphorylation. t. vaginalis hydrogenosomes lack a genome and translation machinery, hence they import all their proteins from the cytosol, but to date only 30 imported proteins have been ... | 2011 | 22140228 |
| the antibacterial activity of ll-37 against treponema denticola is dentilisin-protease independent and facilitated by the major outer sheath protein virulence factor. | host defense peptides are innate immunoeffectors that possess both bactericidal activities and immunomodulatory functions. deficiency in the human ll-37 host defense peptide has been previously correlated with severe periodontal disease. treponema denticola is an oral anaerobic spirochete closely associated with the pathogenesis of periodontal disease. the t. denticola major surface protein (msp), involved in adhesion and in cytotoxicity, and the dentilisin serine protease are key virulence fact ... | 2011 | 22184422 |
| relationship between periodontal diseases and preterm birth: recent epidemiological and biological data. | for ten years, the incidence of preterm birth does not decrease in developed countries despite the promotion of public health programs. many risk factors have been identified including ethnicity, age, tobacco, and infection. however, almost 50% of preterm birth causes remain unknown. the periodontal diseases are highly prevalent inflammatory and infectious diseases of tooth supporting tissues leading to an oral disability. they influence negatively general health worsening cardiovascular disease ... | 2011 | 22132334 |
| salivary levels of antibacterial peptide (ll-37/hcap-18) and cotinine in periodontitis patients. | background: the purpose of this study was to examine the relationship between salivary ll-37 levels and clinical severity in chronic periodontitis (cp) patients. the presence/absence of four periodontopathic bacteria and salivary cotinine levels were also examined in order to assess the impact of these factors on ll-37 production. methods: unstimulated salivary samples were collected from 69 cp patients. salivary concentrations of ll-37 and cotinine were measured by enzyme-linked immunosorbent ... | 2011 | 21942788 |
| characterization and scope of s-layer protein o-glycosylation in tannerella forsythia. | cell surface glycosylation is an important element in defining the life of pathogenic bacteria. tannerella forsythia is a gram-negative, anaerobic periodontal pathogen inhabiting the subgingival plaque biofilms. it is completely covered by a two-dimensional crystalline surface layer (s-layer) composed of two glycoproteins. although the s-layer has previously been shown to delay the bacterium's recognition by the innate immune system, we characterize here the s-layer protein o-glycosylation as a ... | 2011 | 21911490 |
| Porphyromonas gingivalis: a clonal pathogen?: Diversities in housekeeping genes and the major fimbriae gene. | The introduction of multilocus sequence typing (MLST) in infectious disease research has allowed standardized typing of bacterial clones. Through multiple markers around the genome, it is possible to determine the sequence type (ST) of bacterial isolates to establish the population structure of a species. For the periodontal pathogen, Porphyromonas gingivalis, the MLST scheme has been established at www.pubmlst.org/pgingivalis, and data from the database indicate a high degree of genetic diversi ... | 2011 | 22125739 |
| Protease inhibitor levels in periodontal health and disease. | Kretschmar S, Yin L, Roberts F, London R, Flemmig TT, Arushanov D, Kaiyala K, Chung WO. Protease inhibitor levels in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01425.x. © 2011 John Wiley & Sons A/S Background and Objective: Our previous study showed that protease inhibitors were attenuated by the periodontal pathogen Porphyromonas gingivalis in cultured gingival epithelial cells. We hypothesize that fewer protease inhibitors would be present in more adva ... | 2011 | 22029638 |
| Protease inhibitor levels in periodontal health and disease. | Kretschmar S, Yin L, Roberts F, London R, Flemmig TT, Arushanov D, Kaiyala K, Chung WO. Protease inhibitor levels in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01425.x. © 2011 John Wiley & Sons A/S Background and Objective: Our previous study showed that protease inhibitors were attenuated by the periodontal pathogen Porphyromonas gingivalis in cultured gingival epithelial cells. We hypothesize that fewer protease inhibitors would be present in more adva ... | 2011 | 22029638 |
| role of sialidase in glycoprotein utilization by tannerella forsythia. | the major bacterial pathogens associated with periodontitis include tannerella forsythia. we previously discovered that sialic acid stimulates biofilm growth of t. forsythia, and that sialidase activity is key to utilization of sialoconjugate sugars and is involved in host-pathogen interactions in vitro. the aim of this work was to assess the influence of the nanh sialidase on initial biofilm adhesion and growth in experiments where the only source of sialic acid was sialoglycoproteins or human ... | 2011 | 21885482 |
| Conservation of the C-type lectin fold for massive sequence variation in a Treponema diversity-generating retroelement. | Anticipatory ligand binding through massive protein sequence variation is rare in biological systems, having been observed only in the vertebrate adaptive immune response and in a phage diversity-generating retroelement (DGR). Earlier work has demonstrated that the prototypical DGR variable protein, major tropism determinant (Mtd), meets the demands of anticipatory ligand binding by novel means through the C-type lectin (CLec) fold. However, because of the low sequence identity among DGR variabl ... | 2011 | 21873231 |
| the effect of spiramycin on porphyromonas gingivalis and other "classic" periopathogens. | in clinical trials, spiramycin has shown additional benefit overscaling and root planing on pocket depth reduction, but its effect on periodontal microbiota was evaluated only by darkfield microscopy. therefore, this study was conducted to determine the effect of spiramycin administration on porphyromonas gingivalis and other periodontopathic bacteria using 16s rarn pcr technique. thirty two non-smoker adults with untreated periodontitis and pocket depth > or = 7 mm. were evaluated to participat ... | 2011 | 22010417 |
| clinical and microbiological evaluation, by real-time pcr, of non-surgical treatment of aggressive periodontitis associated with amoxicillin and metronidazole. | background: to evaluate the clinical and microbiological changes resulting from non-surgical periodontal treatment associated with amoxicillin and metronidazole, in individuals with aggressive periodontitis (agp). methods: 15 subjects with agp received non-surgical periodontal treatment and 45 days after completion of treatment, were treated with antibiotics. clinical data and samples of subgingival plaque were collected at baseline, 45 days after the non-surgical periodontal treatment and 1 mon ... | 2011 | 22060046 |
| symptomatic and asymptomatic apical periodontitis associated with red complex bacteria: clinical and microbiological evaluation. | in this study, the association of red complex (rc) bacteria that include treponema denticola, tannerella forsythia and porphyromonas gingivalis with acute, exacerbated or chronic apical periodontitis was evaluated. seventy-one patients with periapical disease were evaluated by clinical examination and microbiological samples obtained from the root canals were analyzed by a polymerase chain reaction assay. twenty-one (29.6%) samples were positive for rc bacteria, with t. ... | 2011 | 22143381 |
| tobacco smoke augments porphyromonas gingivalis-streptococcus gordonii biofilm formation. | smoking is responsible for the majority of periodontitis cases in the us and smokers are more susceptible than non-smokers to infection by the periodontal pathogen porphyromonas gingivalis. p. gingivalis colonization of the oral cavity is dependent upon its interaction with other plaque bacteria, including streptococcus gordonii. microarray analysis suggested that exposure of p. gingivalis to cigarette smoke extract (cse) increased the expression of the major fimbrial antigen (fima), but not the ... | 2011 | 22110637 |
| genetic organisation, mobility and predicted functions of genes on integrated, mobile genetic elements in sequenced strains of clostridium difficile. | clostridium difficile is the leading cause of hospital-associated diarrhoea in the us and europe. recently the incidence of c. difficile-associated disease has risen dramatically and concomitantly with the emergence of 'hypervirulent' strains associated with more severe disease and increased mortality. c. difficile contains numerous mobile genetic elements, resulting in the potential for a highly plastic genome. in the first sequenced strain, 630, there is one proven conjugative transposon (ctn) ... | 2011 | 21876735 |
| Associations of periodontal microorganisms with salivary proteins and MMP-8 in gingival crevicular fluid. | OBJECTIVE: We investigated in subjects with and without periodontitis, the levels of certain salivary proteins and matrix metalloproteinase-8 (MMP-8) in gingival crevicular fluid (GCF), in relation to the presence of specific periodontal pathogens. METHODS: Clinical parameters were recorded at baseline, in 1985 and in 2009 from 99 subjects; 56 with and 43 without periodontitis (mean age 59.2 ± SD 2.9). Saliva samples collected in 2009 were analysed for salivary albumin, total protein and immunog ... | 2011 | 22103335 |
| competitive selection of lactic acid bacteria that persist in the human oral cavity. | lactic acid bacteria (lab) might offer opportunities as oral probiotics provided candidate strains persist in the mouth. after intake of a mixture of 69 lab, strains of lactobacillus fermentum and lactobacillus salivarius were especially recovered. coaggregation with other microbes is likely not a prerequisite for persistence since l. salivarius strongly coaggregated with typical oral cavity isolates, whereas l. fermentum failed to display this phenotype. | 2011 | 21965405 |
| microbiologic findings 1 year after partial- and full-mouth scaling in the treatment of moderate chronic periodontitis. | to investigate the impact on microbiologic variables of full-mouth scaling (fms) and conventional scaling and root planing (csrp) after 12 months. | 2011 | 21909493 |
| microbiologic changes in subgingival plaque before and during the early period of orthodontic treatment. | abstract objective: to evaluate changes in subgingival microbiota before and during the leveling and alignment orthodontic stage using the polymerase chain reaction (pcr) method. materials and methods: thirty orthodontic patients (17 females and 13 males; aged 16.7 -¦ 6.5-áy) were included in this study. subgingival microbial samples were taken from the disto-buccal gingival crevice of the left upper central incisors, the left lower central incisors, the mesio-buccal gingival crevice of the left ... | 2011 | 21827233 |
| [prevalence of helicobacter pylori detected by real-time pcr in the subgingival plaque of patients with chronic periodontitis]. | helicobacter pylori plays a significant role in gastric disease. the aim of the present pilot study was to evaluate the prevalence of h. pylori detected by real-time polymerase chain reaction (real-time pcr) in the sub-gingival plaque of chronic periodontitis patients and to determine periodontopathogens profile of positive sites at h. pylori. | 2011 | 21688602 |
| clinical, microbiologic, and immunologic factors of orthodontic treatment-induced gingival enlargement. | the aim of this study was to investigate the microbiologic and immunologic factors related to orthodontic treatment-induced gingival enlargement (ge). | 2011 | 21724088 |
| comparative genome analysis and genome-guided physiological analysis of roseobacter litoralis. | roseobacter litoralis och149, the type species of the genus, and roseobacter denitrificans och114 were the first described organisms of the roseobacter clade, an ecologically important group of marine bacteria. both species were isolated from seaweed and are able to perform aerobic anoxygenic photosynthesis. | 2011 | 21693016 |
| alzheimer's disease - a neurospirochetosis. analysis of the evidence following koch's and hill's criteria. | abstract: it is established that chronic spirochetal infection can cause slowly progressive dementia, brain atrophy and amyloid deposition in late neurosyphilis. recently it has been suggested that various types of spirochetes, in an analogous way to treponema pallidum, could cause dementia and may be involved in the pathogenesis of alzheimer's disease (ad). here, we review all data available in the literature on the detection of spirochetes in ad and critically analyze the association and causa ... | 2011 | 21816039 |
| synergistic virulence of porphyromonas gingivalis and treponema denticola in a murine periodontitis model. | chronic periodontitis is characterized by the destruction of the tissues supporting the teeth and has been associated with the presence of a subgingival polymicrobial biofilm containing porphyromonas gingivalis and treponema denticola. we have investigated the potential synergistic virulence of p. gingivalis and t. denticola using a murine experimental model of periodontitis. an inoculation regime of four intra-oral doses of 1 +ù 10(10) p. gingivalis cells induced significant periodontal bone lo ... | 2011 | 21729244 |
| Simultaneous detection of periodontal pathogens in subgingival plaque and placenta of women with hypertension in pregnancy. | BACKGROUND: There are many studies documenting increased prevalence of periodontal infection in women with preeclampsia. But, very few studies have attempted to establish causal relationship between the two. OBJECTIVE: To find out causal circumstantial evidence by isolating specific periodontal pathogens in oral and placental samples. MATERIALS AND METHODS: Antenatal periodontal screening and subgingival plaque collection was carried out in ten women with hypertension in pregnancy and ten normot ... | 2011 | 21830010 |
| Isolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA). | The human oral cavity is host to a complex microbial community estimated to comprise >700 bacterial species, of which at least half are thought to be not yet cultivable in vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowed the isolation of plasmids from human oral supra- and subgingival plaque samples. Thirty-two novel plasmids and a circular molecule that could be an integrase-generated circular intermediate were isolated. | 2011 | 21711368 |
| filifactor alocis has virulence attributes that can enhance its persistence under oxidative stress conditions and mediate invasion of epithelial cells by porphyromonas gingivalis. | filifactor alocis, a gram-positive anaerobic rod, is one of the most abundant bacteria identified in the periodontal pockets of periodontitis patients. there is a gap in our understanding of its pathogenicity and ability to interact with other periodontal pathogens. to evaluate the virulence potential of f. alocis and its ability to interact with porphyromonas gingivalis w83, several clinical isolates of f. alocis were characterized. f. alocis showed nongingipain protease and sialidase activitie ... | 2011 | 21825062 |
| the rela/spot homolog (rsh) superfamily: distribution and functional evolution of ppgpp synthetases and hydrolases across the tree of life. | rela/spot homologue (rsh) proteins, named for their sequence similarity to the rela and spot enzymes of escherichia coli, comprise a superfamily of enzymes that synthesize and/or hydrolyze the alarmone ppgpp, activator of the "stringent" response and regulator of cellular metabolism. the classical "long" rshs rel, rela and spot with the ppgpp hydrolase, synthetase, tgs and act domain architecture have been found across diverse bacteria and plant chloroplasts, while dedicated single domain ppgpp- ... | 2011 | 21858139 |
| polymicrobial periodontal pathogen transcriptomes in calvarial bone and soft tissue. | porphyromonas gingivalis, treponema denticola, and tannerella forsythia are consistently associated with adult periodontitis. this study sought to document the host transcriptome to a p. gingivalis, t. denticola, and t.forsythia challenge as a polymicrobial infection using a murine calvarial model of acute inflammation and bone resorption. mice were infected with p. gingivalis, t. denticola, and t. forsythia over the calvaria, after which the soft tissues and calvarial bones were excised. a muri ... | 2011 | 21896157 |
| deciphering morphological determinants of the helix-shaped leptospira. | leptospira spp. are thin, highly motile, slow-growing spirochetes that can be distinguished from other bacteria on the basis of their unique helical shape. defining the mechanisms by which these bacteria generate and maintain this atypical morphology should greatly enhance our understanding of the fundamental physiology of these pathogens. in this study, we showed that peptidoglycan sacculi from leptospira spp. retain the helical shape of intact cells. interestingly, the distribution of muropept ... | 2011 | 21926230 |
| distinct and complex bacterial profiles in human periodontitis and health revealed by 16s pyrosequencing. | periodontitis has a polymicrobial etiology within the framework of a complex microbial ecosystem. with advances in sequencing technologies, comprehensive studies to elucidate bacterial community differences have recently become possible. we used 454 sequencing of 16s rrna genes to compare subgingival bacterial communities from 29 periodontally healthy controls and 29 subjects with chronic periodontitis. amplicons from both the v1-2 and v4 regions of the 16s gene were sequenced, yielding 1 393 57 ... | 2011 | 22170420 |
| distinct and complex bacterial profiles in human periodontitis and health revealed by 16s pyrosequencing. | periodontitis has a polymicrobial etiology within the framework of a complex microbial ecosystem. with advances in sequencing technologies, comprehensive studies to elucidate bacterial community differences have recently become possible. we used 454 sequencing of 16s rrna genes to compare subgingival bacterial communities from 29 periodontally healthy controls and 29 subjects with chronic periodontitis. amplicons from both the v1-2 and v4 regions of the 16s gene were sequenced, yielding 1 393 57 ... | 2011 | 22170420 |
| Treponema denticola major outer sheath protein induces actin assembly at free barbed ends by a PIP2-dependent uncapping mechanism in fibroblasts. | The major outer sheath protein (Msp) of Treponema denticola perturbs actin dynamics in fibroblasts by inducing actin reorganization, including subcortical actin filament assembly, leading to defective calcium flux, diminished integrin engagement of collagen, and retarded cell migration. Yet, its mechanisms of action are unknown. We challenged Rat-2 fibroblasts with enriched native Msp. Msp activated the small GTPases Rac1, RhoA and Ras, but not Cdc42, yet only Rac1 localized to areas of actin re ... | 2011 | 21901132 |
| Filifactor alocis interactions with gingival epithelial cells. | An association between the gram-positive anaerobe Filifactor alocis and periodontal disease has recently emerged; however, possible pathogenic mechanisms have not been investigated. In this study we examined the responses of primary cultures of gingival epithelial cells (GECs) to infection with F. alocis. Secretion of the pro-inflammatory cytokines interleukin-1ß, interleukin-6 and tumor necrosis factor-a from GECs was stimulated by F. alocis infection. F. alocis also induced apoptosis in GECs t ... | 2011 | 22053964 |
| immune evasion strategies of porphyromonas gingivalis. | porphyromonas gingivalis is strongly correlated with chronic periodontitis. its chronic persistence in the periodontium depends on its ability to evade host immunity without inhibiting the overall inflammatory response, which is actually beneficial for this and other periodontal bacteria. indeed, the inflammatory exudate (gingival crevicular fluid) is a source of essential nutrients, such as peptides and hemin-derived iron. in this review, i discuss how p. gingivalis can promote its adaptive fit ... | 2011 | 22162663 |
| association of red complex, a. actinomycetemcomitans and non-oral bacteria with periodontal diseases. | pathogens related to systemic infections have been detected in the periodontal microbiota. the relationship amongst these pathogens, periodontal bacteria and periodontal clinical status is poorly understood. this study evaluated the association amongst red complex, a. actinomycetemcomitans (a.a) and non-oral pathogenic bacteria in subjects with good periodontal health (ph), gingivitis (g), chronic (cp) and aggressive (ap) periodontitis. | 2011 | 21397893 |
| driving forces enable high-titer anaerobic 1-butanol synthesis in escherichia coli. | 1-butanol, an important chemical feedstock and advanced biofuel, is produced by clostridium species. various efforts have been made to transfer the clostridial 1-butanol pathway into other microorganisms. however, in contrast to similar compounds, only limited titers of 1-butanol were attained. in this work, we constructed a modified clostridial 1-butanol pathway in escherichia coli to provide an irreversible reaction catalyzed by trans-enoyl-coenzyme a (coa) reductase (ter) and created nadh and ... | 2011 | 21398484 |
| saliva/pathogen biomarker signatures and periodontal disease progression. | the purpose of this study was to determine the role of saliva-derived biomarkers and periodontal pathogens during periodontal disease progression (pdp). one hundred human participants were recruited into a 12-month investigation. they were seen bi-monthly for saliva and clinical measures and bi-annually for subtraction radiography, serum and plaque biofilm assessments. saliva and serum were analyzed with protein arrays for 14 pro-inflammatory and bone turnover markers, while qpcr was used for de ... | 2011 | 21406610 |