Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| persistent infection of hepatitis e virus transmitted by blood transfusion in a patient with t-cell lymphoma. | aim: with advent of reverse-transcription (rt)/polymerase chain reaction (pcr) for detection of the hepatitis e viral genome, we carried out retrospective examinations. methods: serum samples collected from 68 patients diagnosed as viral hepatitis with unknown etiology were tested for viral markers of hepatitis virus. results: two of them were found positive for hepatitis e viral rna. while the clinical course of one patient (patient 1) was typical as acute hepatitis e, another patient (patient ... | 2007 | 17300706 |
| a case of acute hepatitis e associated with multidrug hypersensitivity and cytomegalovirus reactivation. | a 65-year-old japanese man was hospitalized because of acute hepatitis and severe cholestasis due to hepatitis e virus (hev) infection combined with a drug reaction to a cold preparation. he died of disseminated intravascular coagulation and severe intestinal bleeding due to systemic cytomegalovirus reactivation following the development of severe eruptions with marked eosinophilia due to drug hypersensitivity to taurine and ursodeoxycholate preparations. the close interaction between viral infe ... | 2007 | 17300712 |
| new enteroviruses, ev-93 and ev-94, associated with acute flaccid paralysis in the democratic republic of the congo. | surveillance of acute flaccid paralysis often identifies enteroviruses not typeable by virus neutralization in cell culture. during 2000 and 2001, 186 isolates from 138 children with acute flaccid paralysis in the democratic republic of the congo were sent for typing to the national reference centre for enteroviruses in lyon, france. the 5' utr of the viral genome could be amplified by pcr for 158 isolates from 114 patients. isolates from 89 patients were neutralizable, and contained non-polio e ... | 2007 | 17311342 |
| increased hepatitis e virus prevalence on dutch pig farms from 33 to 55% by using appropriate internal quality controls for rt-pcr. | pigs have been suggested to be a potential reservoir for locally acquired human hepatitis e virus (hev) infections in the netherlands. to study possible trends in hev prevalence in the dutch pig population, 97 pig farms have been screened for the presence of hev in stools. the prevalence rate of hev was estimated at 55% (53/97) in 2005, indicating a significant increase as compared to the prevalence rate of 22% (25/115) as was reported in 1999. the current data suggest that this increase is due ... | 2007 | 17320980 |
| detection of swine hepatitis e virus in the porcine hepatic lesion in jeju island. | swine hepatitis e virus (hev) is an emerging zoonotic pathogen due to its close genomic similarity to human hev. the prevalence of swine hev in the hepatic lesion of pigs from the jeju island was investigated by reverse transcriptase polymerase chain reaction (rt-pcr). in total, 40 pigs with hepatitis lesions were selected from 19 different farms, based on examination by microscopy. rtpcr findings revealed swine hev in 22 cases (55%), including 18 suckling pigs and 4 growing pigs. several histop ... | 2007 | 17322774 |
| enterovirus 94, a proposed new serotype in human enterovirus species d. | the genus enterovirus (family picornaviridae) contains five species with strains isolated from humans: human enterovirus a (hev-a), hev-b, hev-c, hev-d and poliovirus. in this study, a proposed new serotype of hev-d was characterized. four virus strains were isolated from sewage in egypt and one strain from acute flaccid paralysis cases in the democratic republic of the congo. the complete genome of one environmental isolate, the complete coding sequence of one clinical isolate and complete vp1 ... | 2007 | 17325357 |
| development and evaluation of an efficient cell-culture system for hepatitis e virus. | using a faecal suspension with high load of hepatitis e virus (hev) (2.0x10(7) copies ml-1, genotype 3), we developed an efficient cell-culture system for hev in a hepatocarcinoma cell line (plc/prf/5). hev progeny released in the culture medium were passaged five times successively in plc/prf/5 cells. the initial day of appearance and load of hev detectable in the culture supernatant after inoculation were dependent on the titre of seed virus in the inoculum. when 6.4x10(4) copies of hev were i ... | 2007 | 17325363 |
| detection and characterization of infectious hepatitis e virus from commercial pig livers sold in local grocery stores in the usa. | hepatitis e virus (hev) is a zoonotic pathogen of which pigs are reservoirs. to determine the presence of hev rna in commercial pig livers sold in local grocery stores in the usa, 127 packages of commercial pig liver were purchased and tested by a universal rt-pcr assay capable of detecting all four known hev genotypes. among the 127 livers tested, 14 were positive for hev rna. sequence and phylogenetic analyses revealed that the 14 isolates all belonged to genotype 3. an animal study was subseq ... | 2007 | 17325364 |
| safety and efficacy of a recombinant hepatitis e vaccine. | hepatitis e virus (hev) is an important cause of viral hepatitis. we evaluated the safety and efficacy of an hev recombinant protein (rhev) vaccine in a phase 2, randomized, double-blind, placebo-controlled trial. | 2007 | 17329696 |
| hepatitis e vaccine--ready for prime time? | 2007 | 17329703 | |
| liver histology in patients with sporadic acute hepatitis e: a study of 11 patients from south-west france. | hepatitis e virus is a ribonucleic acid (rna) enterically transmitted virus that causes both epidemics and sporadic cases of acute hepatitis e in many countries of asia and africa. domestically acquired (non-travel-associated) hepatitis e has been reported recently in many industrialized countries including the usa, europe, and japan. there is little information available on liver histology in these patients. we report a series of 11 patients with sporadic acute hepatitis e and needle liver hist ... | 2007 | 17333266 |
| using improved technology for filter paper-based blood collection to survey wild sika deer for antibodies to hepatitis e virus. | recent reports from japan implicated wild sika deer (cervus nippon) in the zoonotic transmission of hepatitis e to humans. seroprevalence studies were performed to determine if imported feral populations of sika deer in maryland and virginia posed a similar risk of transmitting hepatitis e virus (hev). hunters collected blood on filter paper discs from freshly killed deer. the discs were desiccated and delivered to a collection point. the dried filters were weighed to estimate the amount of bloo ... | 2007 | 17336401 |
| [hepatitis e virus antibodies in the macaques and in the personnel serving the macaques of the adler apery]. | there is evidence for the rather high detection rate of antibodies against hepatic e virus (hev) was rather high in the macaques from the adler apery in 1999-2005. anti-hev was detected in 232 (57.3%) out of 405 examined rhesus macaques (macaca mulata) and in 16 (16%) out of 100 java ones (m. fascicularis). the detection rate of anti-hev ranged from 12.5 to 89.5%% among the rhesus macaques and from 5.9 to 37.5% among the java ones. class m anti-hev was found only in 3 (4.3%) out of 69 java macaq ... | 2007 | 17338232 |
| acute polyarthritis revealing hepatitis e. | we report a case of acute hepatitis e occurring in a 51-year-old french woman, revealed by an abrupt onset of polyarthritis involving the ankles and knees followed by the wrists and fingers. polyarthritis lasted up to 3 months without recurrence. our case was characterised by a 9-month prolonged viraemia with persistence of specific igm in the serum. the hepatitis e virus belonged to genotype 3 and may have been contracted in france or during travel to hyperendemic areas. our case shows that acu ... | 2007 | 17340044 |
| use of serological assays for diagnosis of hepatitis e virus genotype 1 and 3 infections in a setting of low endemicity. | because of the occurrence of genotype 3 hepatitis e virus (hev) in regions of low endemicity, it is important to validate the currently used serological assays for diagnosing infections with viruses belonging to this lineage, since these assays only use antigens derived from genotype 1 and 2 viruses. we evaluated the genelabs enzyme-linked immunosorbent assay (elisa) and the recomblot from mikrogen for the detection of hev-specific immunoglobulin m (igm) and igg under conditions of low endemicit ... | 2007 | 17360853 |
| wild boar as an important reservoir of hepatitis e virus in western japan. | 2007 | 17362297 | |
| prevalence of hepatitis e virus among wild boar in the ehime area of western japan. | aims: transmission of hepatitis e virus (hev) from wild boar to humans has been reported, particularly from japan. we attempted to clarify this issue. methods: we assessed the igg class antibodies against hev (anti-hev) in serum samples taken from 406 boar living in the ehime area of western japan from 2001 to 2004, of which 392 were captured in the wild (wild-caught boar) and 14 had been kept in a breeding farm (bred boar). results: anti-hev positive rate in the bred boar (10/14, 71.4%) was sig ... | 2007 | 17362304 |
| genetic variability and evolution of hepatitis e virus. | hepatitis e virus (hev) is the sole member of the genus hepevirus in the family hepeviridae. hev is transmitted primarily by the fecal-oral route, and water-borne epidemics are characteristic of hepatitis e in many developing countries in asia, africa and latin america where sanitation conditions are suboptimal. accumulating lines of evidence indicate that hev-associated hepatitis also occurs domestically among individuals in industrialized countries, that there are animal reservoirs of hev such ... | 2007 | 17363102 |
| [expression of orf2 protein of hev genotype iv in hansenula polymorpha]. | hepatitis e, an acute infectious disease transmitted via the fecal-oral route, is caused by hepatitis e virus. however, no effective treatment currently exists for hepatitis e, and the only epidemic control approach is vaccination. but so for there are no commercial vaccine for hepatitis e available in the world. to find a new expression system to develop recombinant hepatitis e vaccine, in this study the expression system of methylotrophic yeast hansenula polymorpha was used to express the gene ... | 2007 | 17366891 |
| presence and significance of transfusion-transmitted virus infection in iranian patients on maintenance hemodialysis. | transfusion-transmitted virus (ttv), a recently discovered dna virus, was first identified in patients with non-a to -g hepatitis following blood transfusion. transmission is generally via the parenteral route but recent data suggest that ttv can also be transmitted by the fecal-oral route. | 2007 | 17446957 |
| ongoing subclinical infection of hepatitis e virus among blood donors with an elevated alanine aminotransferase level in japan. | ongoing subclinical infection of hepatitis e virus (hev) has not been fully studied. in the present study, serum samples were collected from 6700 voluntary blood donors with an elevated alanine aminotransferase (alt) level of 61-476 iu/l at a japanese red cross blood center, and were tested for the presence of igg, igm and iga classes of antibodies to hev (anti-hev) by in-house elisa and hev rna by nested rt-pcr. overall, 479 blood donors (7.1%) were positive for anti-hev igg, including 8 donors ... | 2007 | 17457924 |
| presence of hepatitis e virus in a naturally infected swine herd from nursery to slaughter. | hepatitis e virus has recently been recognized as having zoonotic potential and could be transmitted from pig to human. pigs are identified as a potential animal reservoir and hev is highly prevalent in the swine population around the world. in this study, the presence of hev was investigated in 51 subjects reared on a simulated commercial farm setting from the age of 2 weeks up to slaughter. samples were collected on four occasions: at 2, 8, and 18 weeks and between 22-29 weeks of age. anti-hev ... | 2007 | 17459506 |
| immunogenicity of candidate hepatitis e virus dna vaccine expressing complete and truncated orf2 in mice. | hepatitis e virus (hev) is a major cause of enterically transmitted acute hepatitis of adults in developing nations. our present studies show that, the complete orf2 gene (1-660 amino acids, a.a.) coding for capsid protein of hev as candidate dna vaccine induced significant specific humoral and cellular immune responses in mice. gene gun based dna administration led to higher seroconversion rates and hev-specific antibody titers as against needle-injection method. the region (458-607a.a.) within ... | 2007 | 17459540 |
| prevalence of anti-hepatitis e virus antibodies in french blood donors. | accumulating evidence suggests that hepatitis e virus (hev) infection is an emerging disease in regions where hev is nonendemic. in france, the prevalence of anti-hev antibodies in the general population has never been studied. using blood donors' samples, we have found a prevalence of 3.20%, which is similar to that of other industrialized countries. | 2007 | 17460057 |
| [priming with an hev th epitope can improve the humoral immunogenicity of its native protein]. | a dominant h-2d restricted th epitope p34 was found to be contained in recombinant particulate hepatitis e virus (hev) vaccine hev 239. in this paper, the cellular immune response induced in p34 immunized balb/c mice were studied and the priming effect of p34 was characterized. groups of balb/c mice were subcutaneously (s. c.) immunized with p34, splenocytes were then stimulated with p34 and hev 239 protein, cellular immune response was assayed by ifn-gamma-elispot, flow cytometry and t cell pro ... | 2007 | 17460907 |
| [epidemiological surveillance for viral pollution of rivers in toyama prefecture]. | virus pollution of three rivers in toyama prefecture was surveyed over a long period in order to predict and to prevent water-born infection. | 2007 | 17461029 |
| helicobacter pylori, hepatitis viruses a, c, e, antibodies and hbsag - prevalence and associated risk factors in pediatric communities of karachi. | objective: to document the prevalence of helicobacter pylori (h. pylori), hepatitis a virus (hav), hepatitis c virus (hcv), hepatitis e virus (hev) antibodies and hepatitis b virus surface antigen (hbsag), in the pediatric age group of low socioeconomic urban communities of karachi and to identify risk factors associated with these infections. design: cross-sectional survey. place and duration of study: three selected squatter settlements of karachi during april 2002 to december 2004. patients a ... | 2007 | 17462174 |
| locally acquired hepatitis e in the netherlands: associated with the consumption of raw pig meat? | we here present 2 patients who developed hepatitis e, without having been abroad or in contact with anyone who did, indicating locally acquired hepatitis e. we point out that the consumption of raw pig meat could be of relevance in hev-associated hepatitides in the netherlands. | 2007 | 17464870 |
| envelope-receptor interactions in nipah virus pathobiology. | nipah (niv) and hendra (hev) viruses are members of the newly defined henipavirus genus of the paramyxoviridae. nipah virus (niv) is an emergent paramyxovirus that causes fatal encephalitis in up to 70% of infected patients, and there is increasing evidence of human-to-human transmission. niv is designated a priority pathogen in the niaid biodefense research agenda, and could be a devastating agent of agrobioterrorism if used against the pig farming industry. endothelial syncytium is a pathognom ... | 2007 | 17470911 |
| characterization of monoclonal antibodies to hepatitis e virus (hev) capsid protein and identification of binding activity. | twenty-seven monoclonal antibodies (mabs) recognizing the open reading frame 2 structural protein of the pakistan strain of hepatitis e virus (hev) were generated by conventional hybridoma technique. these mabs were characterized by elisa, affinity-capture reverse transcriptase-polymerase chain reaction (ac/rt-pcr), immune electron microscopy (iem), and a rt-pcr based seroneutralization assay. twenty-seven mabs were positive by elisa. by ac/rt-pcr, 24 mabs bound to pakistan and namibia hev strai ... | 2007 | 17487571 |
| the hepatitis e virus orf3 protein protects cells from mitochondrial depolarization and death. | the biology and pathogenesis of hepatitis e virus are poorly understood due to the lack of an in vitro culture or infection models. the viral orf3 protein activates the cellular mitogen-activated protein kinase pathway and is likely to modulate the host cell environment for efficient viral replication. we screened for cellular genes whose transcription was differentially up-regulated in an orf3-expressing stable cell line (orf3/4). the gene for mitochondrial voltage-dependent anion channel (vdac ... | 2007 | 17488721 |
| hepatitis e in new zealand. | locally acquired hepatitis e virus (hev) infection has been described in a number of developed countries and is thought to be zoonotic from pigs. little is known about hepatitis e in humans in new zealand (nz) but 90% of nz pigs show evidence of hev infection. the aim of this study was to investigate the epidemiology of hepatitis e infection in nz by documenting hev immunoglobulin (ig) g seroprevalence in nz blood donors, testing patients with unexplained hepatitis for hev, and comparing genetic ... | 2007 | 17489963 |
| transmission dynamics of hepatitis e among swine: potential impact upon human infection. | hepatitis e virus (hev) infection is a zoonosis for which pigs play a role as a reservoir. in japan, the infection has been enzootic in swine. clarifying the detailed mechanisms of transmission within farms is required in order to facilitate an understanding of the age-specific patterns of infection, especially just prior to slaughter. | 2007 | 17493260 |
| evaluation of human (genotype 1) and swine (genotype 4)-orf2-based elisas for anti-hev igm and igg detection in an endemic country and search for type 4 human hev infections. | open reading frame 2 proteins (orf2) from swine (genotype 4, s-orf2) and human (genotype 1, h-orf2) hepatitis e virus (hev) having 91.4% identity at amino acid level were expressed using baculovirus expression system. comparison of elisas based on the two proteins yielded identical results when sequential serum samples from monkeys and pigs experimentally infected with genotypes 1 and 4 hev, respectively, were tested. samples from patients (n = 258) suffering from non-a, non-b hepatitis during o ... | 2007 | 17501765 |
| seroepidemiology of hepatitis e virus in patients with non-a, non-b, non-c hepatitis in hungary. | many cases of acute hepatitis remain undiagnosed and the hepatitis e virus (hev) is emerging in industrialized countries. the aim of this study was to assess the role hev as causative agent in acute non-a, non-b, and non-c hepatitis patients in hungary. 10.5% of the 264 acute non-a, non-b, and non-c hepatitis patients tested had anti-hev igg and 1.9% had anti-hev igm as tested by elisa. after confirmation by western blot 6.1% of the acute non-a, non-b, and non-c hepatitis patients had anti-hev i ... | 2007 | 17516521 |
| unchanged high prevalence of antibodies to hepatitis e virus (hev) and hev rna among blood donors with an elevated alanine aminotransferase level in japan during 1991-2006. | hepatitis e is rare in japan but is occurring more frequently than previously thought. to investigate whether de novo subclinical infection of hepatitis e virus (hev) has recently increased in japan, hev rna was assayed in serum samples obtained from 4019 japanese voluntary blood donors with alanine aminotransferase (alt) of > or =61 iu/l, who are likely to have ongoing hev infection, during 1991-2006. the overall rates of igg-class antibody to hev (anti-hev igg), anti-hev igm/iga and hev rna am ... | 2007 | 17533550 |
| impact of viral hepatitis on apo- and lipoprotein status in blood. | to investigate the effect of viral hepatitis on serum levels of apo- and lipoproteins in different forms of liver diseases. | 2007 | 17541298 |
| prevalence of antibodies to hepatitis e virus among male blood donors in tabriz, islamic republic of iran. | serum samples from 399 voluntary male blood donors were tested for anti-hepatitis e virus (hev) igg during july and august 2004 at a blood transfusion centre in tabriz city. the prevalence of anti-hev igg was 7.8% (95% ci: 5.2-10.4). risk factors for infection included age and a low educational level. seroprevalence increased significantly with age, from 3.3% in subjects under 30 years of age to 37.5% in individuals 50 years and over. illiterate individuals were at significantly higher risk for ... | 2007 | 17546911 |
| [characterization of antigenic epitopes of orf2 encoded proteins of hepatitis e virus genotype 4 identified in china]. | to characterize antigenic epitopes of hepatitis e virus (hev) genotype 4 that was first identified in china a few years ago, a recombinant protein, p166chn, encoded by hev genotype 4 orf2 was used to prepare anti-p166chn mcabs. simultaneously, twenty n- or c-terminal truncated p166chn proteins were generated. immunoreactivity between the mcabs and the truncated proteins as well as seven p166 recombinant proteins derived from different hev genotypes and subgenotypes was detected by indirect elisa ... | 2007 | 17548336 |
| hepatitis e vaccine. | 2007 | 17554127 | |
| acute viral hepatitis e and japanese encephalitis: an unusual co-occurrence. | 2007 | 17558086 | |
| hepatitis e vaccine. | 2007 | 17561526 | |
| transfusion-associated hepatitis e, france. | 2007 | 17561564 | |
| prevalence of hepatitis e virus antibodies in patients with chronic hepatitis b and chronic hepatitis c. | to investigate the prevalence of hepatitis e virus (hev) among patients with chronic hepatitis b and chronic hepatitis c, serum samples were collected between january and december 2004 from patients with chronic hepatitis b and chronic hepatitis c. | 2007 | 17570930 |
| construction and characterization of the chimeric antibody 8c11 to the hepatitis e virus. | homodimers of the truncated hepatitis e virus (hev) capsid proteins, e2 and p239, were conformed to model the dominant antigenic determinants of hev. using e2 as an immunogen, two neutralizing monoclonal antibodies (mabs), namely 8c11 and 8h3, were produced. we constructed a mouse-human chimeric antibody derived from 8c11 and its expression in chinese hamster ovary (cho) cells. cdnas encoding variable regions of heavy and light chains were isolated from hybridoma cells and inserted into mammalia ... | 2007 | 17645530 |
| [a sero-epidemiology study on hepatitis e virus infection in fujian province]. | to understand the sero-prevalence of hepatitis e virus (hev) infection among different populations and animals in fujian province. | 2007 | 17649675 |
| the histology of acute autochthonous hepatitis e virus infection. | to document the histological appearances of liver biopsies in autochthonous hepatitis e virus (hev) infection. | 2007 | 17650215 |
| molecular determinants of antiviral potency of paramyxovirus entry inhibitors. | hendra virus (hev) and nipah virus (niv) constitute the henipavirus genus of paramyxoviruses, both fatal in humans and with the potential for subversion as agents of bioterrorism. binding of the hev/niv attachment protein (g) to its receptor triggers a series of conformational changes in the fusion protein (f), ultimately leading to formation of a postfusion six-helix bundle (6hb) structure and fusion of the viral and cellular membranes. the ectodomain of paramyxovirus f proteins contains two co ... | 2007 | 17652384 |
| single amino acid changes in the nipah and hendra virus attachment glycoproteins distinguish ephrinb2 from ephrinb3 usage. | the henipaviruses, nipah virus (niv) and hendra virus (hev), are lethal emerging paramyxoviruses. ephrinb2 and ephrinb3 have been identified as receptors for henipavirus entry. niv and hev share similar cellular tropisms and likely use an identical receptor set, although a quantitative comparison of receptor usage by niv and hev has not been reported. here we show that (i) soluble niv attachment protein g (sniv-g) bound to cell surface-expressed ephrinb3 with a 30-fold higher affinity than that ... | 2007 | 17652392 |
| hev prevalence in the general population and among workers at zoonotic risk in latium region. | hepatitis e virus (hev) infections are responsible for large waterborne outbreaks in developing countries. sporadic cases in the developed world are mainly imported from endemic areas. hev has been suggested to be a zoonotic infection, for which pigs may be the reservoir; specific swine strains of hev have been identified. humans are susceptible to infections with swine strains. the aim of this study was to analyse whether italian pig farmers, veterinarians and abattoir workers are more exposed ... | 2007 | 17658105 |
| selective suppression of nf-kbp65 in hepatitis virus-infected pregnant women manifesting severe liver damage and high mortality. | fulminant hepatitis in asian pregnant women is generally caused by hepatitis e virus infection, and extremely high mortality is most common in them. decreased cell-mediated immunity is considered a major cause of death in these cases, but what exactly influences decreased immunity and high mortality specifically during pregnancy is not known. we used electrophoretic mobility shift assays, immunoblotting, and immunohistochemical analysis to study the expression and dna binding activity of nf-kb p ... | 2007 | 17660862 |
| hepatitis a, b, c, d, e, g: an update. | acute and chronic liver diseases are an assortment of disorders brought to the clinician's attention by abnormal liver function tests or specific signs and symptoms. the differential diagnosis includes disorders that have primary or secondary liver involvement. this paper will be limited to the epidemiology, clinical manifestations, diagnosis, treatment, and prevention of the different viral liver diseases: a, b, c, d, e and g. | 2007 | 17684813 |
| latent infection of a new alphanodavirus in an insect cell line. | insect bti-tn-5b1-4 (tn5) cells have been used extensively with recombinant baculoviruses to express foreign genes. when a recombinant baculovirus containing the hepatitis e virus capsid protein gene was used to infect tn5 cells, unknown virus particles in addition to the anticipated hepatitis e virus-like particles were produced in the infected cells. the unknown virus particles were 35 nm in diameter and contained rna that was highly homologous to full-length rna1 (3,107 bp) and rna2 (1,383 bp ... | 2007 | 17686877 |
| a score method for comparison of partial genomic regions in their representatives of full-length genome of hepatitis e virus for genotyping. | in this article, a statistical score method for comparison of partial genomic regions in their representatives of full-length genome of hepatitis e virus (hev) for genotyping was developed. the critical values of the statistics for hypothesis testing were generated through monte carlo simulations. it was found that fragment iii might be a statistically representative region for the whole genome of hev for genotyping. based on both the full-length sequence and fragment iii, the same four genotype ... | 2007 | 17687190 |
| [hepatitis e virus: zoonotic implications]. | the hepatitis e virus (hev) is transmitted primarily by the feco-oral route throughout contaminated water and/or food, and is one of the main causes of acute hepatitis worldwide. hepatitis e shows a high mobility but a low mortality rate, except in pregnant women, where it can be as high as 30%. hev causes sporadic cases and epidemic outbreaks, mainly in africa, asia and central america. in europe, there is an increase in the number of reported autochthonous cases no related with travel to endem ... | 2007 | 17692200 |
| prevalence and genetic characterization of hepatitis e virus in paired samples of feces and serum from naturally infected pigs. | this study describes the distribution of hepatitis e virus (hev) in a naturally infected swine population and the genetic relatedness of hev strains on swine farms in spain. of fecal and serum samples collected from 131 pigs and manure-ditch samples collected from 17 farms, hev was detected in 16%, 14%, and 59%, respectively, for an overall prevalence rate of 23%. the maximum prevalence rates for feces and serum were in pigs 5 to 12 wk old. a high prevalence of the virus in feces (18%) was obser ... | 2007 | 17695601 |
| [acute hepatitis e]. | 2007 | 17700753 | |
| acute pancreatitis in acute viral hepatitis. | to elucidate the frequency and characteristics of pancreatic involvement in the course of acute (nonfulminant) viral hepatitis. | 2007 | 17963301 |
| new trends in emerging pathogens. | the emergence of pathogens is the result of a number of impact in all parts of the food chain. the emerging technologies in food production explain how new pathogens can establish themselves in the food chain and compromise food safety. the impact of the food technology is analysed for several bacteria, such as yersinia, campylobacter, arcobacter, helicobacter pullorum, enterobacter sakazakii, mycobacterium avium spp. paratuberculosis, prions related to vcjd and others. the importance of the abi ... | 2007 | 17976849 |
| first human cases of hepatitis e infection with genotype 3c strains. | 2007 | 17981498 | |
| autochthonous hepatitis e infection in a slaughterhouse worker. | we report the first hepatitis e infection case detected in a slaughterhouse worker. the identified strain belonged to genotype 3, subtype 3f. partial sequence analysis of the strain isolated from his serum showed a percentage of nucleotide homology ranging from 83.4% up to 97.3% compared with european human and swine strains, respectively. these findings point strongly to hepatitis e virus as a vocationally acquired illness by means of the manipulation of infected organs from pigs. | 2007 | 17984349 |
| [presence of anti hepatitis e virus antibodies in swine: is it an animal reservoir for hepatitis e?]. | swine hepatitis e virus (hev) has a cross-reaction with human anti-hev antibodies. therefore, pigs could be an animal reservoir, rendering hepatitis e as a zoonosis. the spread of this infection among infected pigs across countries would be possible through trading. previously, using an anti-human conjugate, we detected anti-hev antobodies in adult pigs in chile. | 2007 | 17989856 |
| evaluation of anti-hepatitis e virus (hev) immunoglobulin a in a serological screening for hev infection. | several formulations of serological diagnostic kits were developed recently in japan for detecting hepatitis e virus (hev) infection. the present study was conducted to evaluate a novel anti-hev serological kit based on detection of class a immunoglobulin antibody (anti-hev iga). | 2007 | 18008036 |
| complete genome sequences for nine simian enteroviruses. | analysis of the vp1 capsid-coding sequences of the simian picornaviruses has suggested that baboon enterovirus (baev), sv19, sv43 and sv46 belong to the species human enterovirus a (hev-a) and sa5 belongs to hev-b, whereas sv4/a2 plaque virus (two isolates of a single serotype), sv6 and n125/n203 (two isolates of a single serotype) appear to represent new species in the genus. we have further characterized by complete genomic sequencing the genetic relationships among the simian enteroviruses se ... | 2007 | 18024906 |
| hepatitis e. | 2007 | 18043454 | |
| acute hepatitis e with severe jaundice: report of three cases. | hepatitis e virus (hev) is the main cause of enterically transmitted non-a hepatitis worldwide. infection is endemic in developing countries. disease course is benign, and severe jaundice is rarely reported. three patients presented to our department with symptomatic acute hepatitis. two of them had recently travelled to endemic areas. jaundice was very marked in all patients. hev infection was documented by hev antibodies and by hev-rna detection in serum and stools. in the autochthonous case, ... | 2007 | 18049173 |
| hepatitis e virus infection in thai troops deployed with u.n. peacekeeping forces. | the hepatitis e virus (hev) is thought to be endemic throughout much of the world, particularly where sanitary infrastructure remains inadequate. hev has been considered a military health threat and has been reported in several military environments. this study determined hev seroconversion (defined by a 4-fold increase in antibody titers) occurring in thai soldiers deployed to the hev-endemic areas of east timor, afghanistan, and iraq, as part of the u. n. multinational forces. with an average ... | 2007 | 18062401 |
| identification of genotype 4 hepatitis e virus strains from a patient with acute hepatitis e and farm pigs in bali, indonesia. | a previous study revealed that antibodies to hepatitis e virus (hev) (anti-hev) are highly prevalent among healthy individuals and farm pigs in bali, indonesia, and suggested that hev infection may occur via zoonosis among balinese people. however, there were no reports of acute hepatitis e in bali. to elucidate whether balinese hev strains recovered from infected humans and pigs have significant sequence similarity, serum samples obtained from 57 patients (age, mean +/- standard deviation, 31.1 ... | 2007 | 17596841 |
| analysis of the full-length genome of hepatitis e virus isolates obtained from farm pigs in mongolia. | although no outbreaks of hepatitis e have been reported in mongolia, a significant proportion of the general population had antibodies to hepatitis e virus (hev). to investigate whether pigs are possible reservoirs of hev in mongolia, serum samples obtained from 243 2- or 3-month-old pigs on four swine farms surrounding ulaanbaatar, the capital city of mongolia, were tested for the presence of anti-hev antibodies and hev rna. overall, 223 pigs (91.8%) tested positive for anti-hev, while 89 pigs ... | 2007 | 17597486 |
| maternal and fetal outcomes in pregnant women with acute hepatitis e virus infection. | hepatitis e virus (hev) infection is known to cause severe liver disease in pregnant women. it is unclear whether obstetric and fetal outcomes are worse in pregnant women with hev infection than in women with other forms of viral hepatitis. | 2007 | 17606958 |
| acute immune thrombocytopenia associated with hepatitis e in an adult. | 2007 | 17616970 | |
| serological evidence of hepatitis e virus infection in an indigenous north american population. | hepatitis e virus (hev) infections are thought to be uncommon in north america. recently, hev transmission has been reported following the consumption of deer meat. because deer are closely related to caribou and caribou meat is a staple of the canadian inuit and the american eskimo diet, the present study explored the seroprevalence of hev infection in an isolated canadian inuit community. | 2007 | 17637946 |
| prolonged fecal shedding of hepatitis e virus (hev) during sporadic acute hepatitis e: evaluation of infectivity of hev in fecal specimens in a cell culture system. | to investigate the duration of fecal shedding and changing loads of hepatitis e virus (hev) in feces and serum from patients with acute hev infection, hev rna was quantitated in periodic serum and fecal specimens obtained from 11 patients with sporadic acute hepatitis e. all 11 patients had detectable hev rna in serum at admission, with the highest viral load being 1.9 x 10(3) to 1.7 x 10(7) copies/ml, and hev viremia lasted until days 17 to 48 (mean, 28.3) after the onset of hepatitis. even at ... | 2007 | 17728471 |
| the emergence of porcine circovirus 2b genotype (pcv-2b) in swine in canada. | since late 2004, the swine industry in the province of quebec has experienced a significant increase in death rate related to postweaning multisystemic wasting syndrome (pmws). to explain this phenomenon, 2 hypotheses were formulated: 1) the presence of a 2nd pathogen could be exacerbating the porcine circovirus 2 (pcv-2) infection, or 2) a new and more virulent pcv-2 strain could be infecting swine. in 2005, 13 pmws cases were submitted to the quebec provincial diagnostic laboratory and pcv-2 w ... | 2007 | 17824323 |
| universal detection of hepatitis e virus by two real-time pcr assays: taqman and primer-probe energy transfer. | hepatitis e virus (hev) is a major cause of food- and waterborne diseases in countries with poor sanitation. furthermore, travellers to such countries are also at risk of contracting the virus. noteworthily, during the last decade an increasing number of non-travel-related cases were recorded even in countries with high sanitary standards. an alternative, direct route of infection, from animals to humans (zoonotic transmission) is suspected to be the cause of recent cases of hepatitis e. in orde ... | 2007 | 17825434 |
| the role of hepatitis e virus testing in drug-induced liver injury. | locally acquired hepatitis e is an emerging infection in developed countries and can be misdiagnosed as drug-induced liver injury. | 2007 | 17850420 |
| characterization of hepatitis e virus from outbreak and sporadic cases in turkmenistan. | large outbreaks and sporadic cases of hepatitis e have been reported in central asia. we assessed the genetic relatedness of hepatitis e virus (hev) strains from outbreak and sporadic cases in turkmenistan. specimens from outbreak and sporadic cases of acute hepatitis non-a, non-b were tested by reverse transcription (rt)-polymerase chain reaction (pcr) to identify the presence of hev rna; nucleotide sequences were analyzed. hev rna was detected from 23/156 (15%) outbreak cases and 2/23 (9%) spo ... | 2007 | 17854031 |
| efficient recombinant hepatitis e virus vaccine: mission accomplished? | 2007 | 17879359 | |
| [detection of the virus of hepatitis e isolates and elucidation of the possibility of their replication in vitro]. | blood serum samples collected from patients with acute hepatitis symptoms admitted to infectious disease hospitals of novosibirsk, barnaul, and irkutsk were studied. the serum samples were tested for the igm and igg antibodies to hev using elisa. seropositive samples were tested using rt-pcr for hev rna. two hev strains were isolated, and thus hev infection was identified for west siberia. one of this strains is classified as hev genotype i; the other, as genotype iii. cell culturing of these st ... | 2007 | 17886472 |
| detection of hepatitis e virus in italian pig herds. | 2007 | 17890772 | |
| [recombinant hev caspid protein p239 specifically attached on hepg2 cells and blocked the infection of wild-type hev on liver cells]. | by using western blot and immunofluorescence assays, the recombinant hev capsid protein p239 was found specifically attached to the hepg2 cell surface and entered to the cytoplasm with the increase of incubation temperature. pre-mixture of wild-type hev with p239 blocked the infectivity of the virus on primary cultured human hepatocytes and hepg2 cells, indicating that p239 and hev competed the same targeting site on these cells. these data provide evidence that p239 has a similar cell surface s ... | 2007 | 17894238 |
| cloning of full genome sequence of hepatitis e virus of shanghai swine isolate using race method. | genotype 4 hepatitis e virus (hev) was reportedly transmitted freely between humans and swine in eastern china. the full-length genomic sequence of shanghai swine isolate (sh-sw-zs1) recovered from feces sample of a pig which was infected with hev rna positive swine serum was determined using rt-pcr and race (rapid amplification of cdna ends) methods. the full genome of the sh-sw-zs1 isolate was 7265 nucleotides in length and phylogenetic analysis indicated that this isolate belonged to genotype ... | 2007 | 17925017 |
| presence of hepatitis e virus in sewage in northern india: frequency and seasonal pattern. | outbreaks of acute hepatitis e, associated with consumption of contaminated drinking water, are frequent in india. sewage is a major source for contamination of surface water. data on the presence of hepatitis e virus (hev) in sewage in india are limited. the aim of this study was to look for the presence of hev rna in concentrates of sewage specimens collected from a major open sewage drain in lucknow, india during august 2004 to july 2006, by the polymerase chain reaction, using primers specif ... | 2007 | 17935174 |
| comparison of real-time fluorescent rt-pcr and conventional rt-pcr for the detection of hepatitis e virus genotypes prevalent in china. | to compare the specificity and sensitivity of a real-time fluorescent rt-pcr assay with conventional rt-pcr, sera from 110 healthy blood donors, 120 patients with a clinical diagnosis of chronic hepatitis b, and 416 patients with non-a-c acute hepatitis, as well as serial dilutions of hev genotypes 1 and 4, were tested with both assays. all samples from healthy blood donors and patients with chronic hepatitis b were negative by both assays. real-time rt-pcr could detect the same final dilution o ... | 2007 | 17935186 |
| [a case of fulminant hepatitis e treated with artificial liver support]. | a 40-year-old man, who had suffered from general malaise and brown urine during his stay in china, was admitted with remarkable jaundice and hepatocellular disorders soon after he returned to japan. because his coagulation test results worsened, he was transferred to our hospital. no evidence of hepatitis a-d virus infection, autoimmune hepatitis, or metabolic disorders was noticed. his prothrombin time was extended (18%), grade ii encephalopathy appeared on the second hospital day, and fulminan ... | 2007 | 17283417 |
| [recent etiology and clinical features of acute viral hepatitis in a single center of korea]. | the etiology of acute viral hepatitis in korea has been dynamically changing during the recent years. the aim of this study was to investigate the recent etiology and the clinical features of acute viral hepatitis in a single center of korea. | 2007 | 18159147 |
| epidemiology of hepatitis b, c, and e viruses and human immunodeficiency virus infections in tahuna, sangihe-talaud archipelago, indonesia. | the epidemiology of hepatitis b, c,e viruses (hbv, hcv, hev) and human immunodeficiency virus(hiv) has been obscure in indonesia, particularly remote areas. | 2007 | 18185013 |
| [attention-getting zoonoses: hepatitis e]. | 2007 | 18069292 | |
| the incidence of sporadic viral hepatitis in north india: a preliminary study. | viral hepatitis is one of the major causes of mortality and morbidity in developing countries. hepatitis e virus (hev) among the major etiological agents is responsible for both sporadic and epidemic outbreaks. the epidemic outbreak is water-borne whereas the sporadic outbreak is possibly through contact. various diagnostic tools at times fail to pinpoint the cause of viral hepatitis. this study was carried out to evaluate the utility of elisa and nested reverse transcriptase polymerase chain re ... | 2007 | 18086624 |
| outbreak of acute viral hepatitis due to hepatitis e virus in hyderabad. | a waterborne outbreak of viral hepatitis occurred in the old city of hyderabad from march through august 2005. an attempt was made to study the outbreak clinically, serologically, and etiologically. | 2007 | 18087089 |
| [the molecular differences between genotype 1 and genotype 4 of hev in their neutralization region]. | hev is classified into h (human) group and z (zoonosis) group according to its compatible host. h group contains genotype 1 and genotype 2 hev isolates which infect human only; z group contains genotype 3 and genotype 4 hev isolates which infect both human and animals. after analysis of amino acid sequences between orf2 aa368 and aa606, four group-conserved sites that were all located in the neutralization region of orf2 were identified. they are aa483, aa492, aa497 and aa599. mutation analysis ... | 2007 | 18092682 |
| acute liver failure caused by hepatitis e virus and paracetamol. | acute liver failure is characterized by severe and sudden liver cell dysfunction leading to hepatic encephalopathy and hepatic coagulopathy in a person without history of liver disease in the past. this catastrophic illness can rapidly progress to coma and death from cerebral edema and multi organ dysfunction. it carries a high mortality rate if liver transplantation is not carried out. in west, paracetamol is the main cause of hepatotoxicity whereas in the east viral hepatitis tops the list. th ... | 2007 | 18094741 |
| hepatitis e virus rna in commercial porcine livers in the netherlands. | human hepatitis e virus (hev) infections by genotype 3 strains in industrialized countries are hypothesized to be caused by pigs. to examine this hypothesis, the potential health risks of transmission routes should be examined. possible foodborne transmission was studied by quantifying the presence and infectivity of hev in commercial porcine livers in the netherlands. a comparison of four tissue disruption and seven rna extraction methods revealed that mechanical disruption followed by silica-b ... | 2007 | 18095450 |
| possible zoonotic transmission of hepatitis e from pet pig to its owner. | hepatitis e is transmitted mainly by water or food, but in industrialized countries, all routes of transmission have not been identified. we describe possible zoonotic transmission of hepatitis e virus that involved direct contact between a pet pig and its owner. | 2007 | 18214190 |
| [autochthonous hepatitis e in france and consumption of raw pig meat]. | viral hepatitis e is an endemic infection in developing countries. emerging cases of autochthonous hepatitis e have been observed in developed countries, especially in france. transmission route of those cases remains unknown and contamination may occur from an animal reservoir. we report two new cases of hepatitis e simultaneously diagnosed in a couple after a trip in southern france. diagnosis was based on detection of anti-hev igm and hev rna in sera of the two patients. epidemiologic investi ... | 2007 | 18176364 |
| [an analysis of the clinical characteristics of patients with chronic hepatitis b superinfected with acute hepatitis e]. | to investigate clinical features of the patients with hepatitis b superinfected with acute hepatitis e (ahe). | 2007 | 18322591 |
| macaque models of human infectious disease. | macaques have served as models for more than 70 human infectious diseases of diverse etiologies, including a multitude of agents-bacteria, viruses, fungi, parasites, prions. the remarkable diversity of human infectious diseases that have been modeled in the macaque includes global, childhood, and tropical diseases as well as newly emergent, sexually transmitted, oncogenic, degenerative neurologic, potential bioterrorism, and miscellaneous other diseases. historically, macaques played a major rol ... | 2008 | 18323583 |
| seroepidemiology and molecular characterization of hepatitis e virus in jilin, china. | in order to develop region-specific diagnostic assays and prevent hepatitis e virus (hev), it is essential to understand epidemiology and genotypic variation within different populations. however, the epidemiological data of hev infections in jilin is shortage. | 2008 | 18330507 |
| [viral hepatitis in travellers]. | considering the geographical asymmetric distribution of viral hepatitis a, b and e, having a much higher prevalence in the less developed world, travellers from developed countries are exposed to a considerable and often underestimated risk of hepatitis infection. in fact a significant percentage of viral hepatitis occurring in developed countries is travel related. this results from globalization and increased mobility from tourism, international work, humanitarian and religious missions or oth ... | 2008 | 18331700 |
| [seroprevalences of hepatitis a and e infections in tunisia]. | viral hepatitis a (hav) and e (hev) infections are still frequent in many regions of the world, particularly in developing countries where sanitary conditions and socioeconomic level are frequently low. in this work, we have studied seroprevalences of these two infections in tunisian children, teenagers and young adults. | 2008 | 18178327 |