Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
human papillomavirus testing in cervical screening. 19957658887
analysis and clinical implications of k-ras gene mutations and infection with human papillomavirus types 16 and 18 in primary adenocarcinoma of the uterine cervix.experimental models indicate that activated ras genes and hpv oncogenic sequences may cooperate in inducing a completely transformed phenotype in epithelial cells. we searched for k-ras gene mutations and hpv type-16 and -18 sequences in 67 primary adenocarcinomas of the uterine cervix by analyzing dnas from formalin-fixed, paraffin-embedded tissue samples. target sequences were amplified by pcr and analyzed by denaturing gradient gel electrophoresis (dgge) and sequencing for the detection of k- ...19957665253
titration of hpv-11 infectivity and antibody neutralization can be measured in vitro.human papillomavirus type 11 (hpv-11), produced from the athymic mouse xenograft system, was shown to infect cultured neonatal human foreskin keratinocytes and the hacat keratinocyte cell line in vitro. infection was documented by the appearance of hpv-11-specific spliced mrna, detected by reverse transcriptase-polymerase chain reaction. purified hpv-11 virions at concentrations of approximately 10(7) particles/ml could successfully evoke infection in this system. infection was completely abroga ...19957665926
sequential activation of cyclin e and cyclin a gene expression by human papillomavirus type 16 e7 through sequences necessary for transformation.to investigate e7-dependent biochemical changes which are involved in cellular transformation, we analyzed the influence of human papillomavirus type 16 (hpv-16) e7 on the expression of cell cycle regulatory proteins. expression of e7 in established rodent fibroblasts (nih 3t3), which was shown to be sufficient for transformation of these cells, leads to constitutive expression of the cyclin e and cyclin a genes in the absence of external growth factors. surprisingly, expression of the cyclin d1 ...19957666540
histogenesis of papillomas of the nose and paranasal sinuses.to determine by review of their histogenesis whether papillomas of the nose and paranasal sinuses are three distinct entities or, as has been favored in the literature, three variations of a single entity.19957668940
prevalence and histologic significance of cervical human papillomavirus dna detected in women at low and high risk for cervical neoplasia.to determine and compare the prevalence and histologic significance of human papillomavirus (hpv) nucleic acids in cervical specimens from women at low (routine hysterectomy) and high (suspicion of cervical neoplasia) risk for cervical neoplasia.19957675368
differential sensitivities of e6 type-specific and l1 consensus primers in the detection of human papillomavirus in anal carcinoma.anogenital malignancy has been increasing in incidence in recent decades. there is strong evidence in the literature suggesting that human papillomavirus (hpv) plays a role in the genesis of anogenital neoplasia. in addition, identification of oncogenic hpv types in anogenital carcinomas may have prognostic significance. the method used to detect hpv infection, however, affects the frequency with which viral dna is identified. we examined tissues from 56 patients with anal squamous cell carcinom ...19957675769
characterization of human aortic smooth muscle cells expressing hpv16 e6 and e7 open reading frames.a comparative study of human papillomavirus type 16 e6e7-transfected and normal human aortic smooth muscle cells by morphological, electron microscopic, immunofluorescent, and biochemical analyses demonstrated that the e6e7-expressing cells retained much of the phenotype of normal aortic smooth muscle cells, including expression of smooth muscle markers and appropriate growth responses to pdgf and heparin. these cells differed from normal vascular smooth muscle cells in that they had slightly al ...19957677186
a cohort study comparing the detection of hpv dna from women who stop and continue to smoke.the objective of this study was to compare the detection of human papillomavirus (hpv) dna in women who stopped smoking, and those who continued to smoke. women entering a smoking cessation programme had tampon specimens collected and analyzed for hpv dna using polymerase chain reaction (pcr) (l1 consensus primers). women with hpv dna in their initial specimens had follow-up specimens collected 1 year later. the stored initial specimens were reanalyzed in the same experiment with the follow-up s ...19957677684
high long-term cure rate justifies routine treatment of cervical intraepithelial neoplasia grade i.the long-term clearance of cervical intraepithelial neoplasia (cin) grade 1 after one treatment was studied retrospectively in 105 patients. treatment was by co2 laser vaporization (74 patients), cone biopsy (8), loop electroexcision (leep) (7), electrodiathermy (1) and hysterectomy (2); 79 of the 92 treated patients (85.9%) had normal cervical cytology and colposcopy on a mean follow-up period of 36.7 months (95% confidence interval between 33.1-40.4 months). life table analysis showed that the ...19957677687
antibodies to human papillomavirus type 16 e7 related to clinicopathological data in patients with cervical carcinoma.to investigate the correlation between antibodies to the transforming protein e7 of human papillomavirus (hpv) type 16 and clinicopathological indices in women with cervical squamous carcinoma.19957629285
comparative analysis of human papillomavirus detection by pcr and non-isotopic in situ hybridisation.aims--to assess the relative diagnostic performance of the polymerase chain reaction (pcr) and non-isotopic in situ hybridisation (nish) and to correlate these data with cytopathological assessment. methods--paired analysis of human papillomavirus (hpv) detection was performed by pcr and nish on exfoliated cervical cells from 122 women attending a routine gynaecological examination. pcr amplification followed by generic and hpv type specific hybridisation was compared with nish on a parallel cer ...19957629286
differential effects by mad and max on transformation by cellular and viral oncoproteins.c-myc is an essential component of the regulatory mechanisms controlling cell growth. max is the obligatory partner of c-myc for all its biological functions analysed to date. recently two max interacting proteins, mad and mxi1, have been identified. it has been suggested that these two proteins modulate c-myc function, in the simplest model by competing with c-myc for the interaction with max. we have analysed different aspects of mad function in comparison to max. native mad/max heterodimers b ...19957630643
polymerase chain reaction. a sensitive indicator of the prevalence of human papillomavirus dna in a population with sexually transmitted disease.a total of 311 cervical samples from first attenders at a sexually transmitted disease clinic assayed for human papillomavirus (hpv) dna with viratype (vt) were analyzed with the polymerase chain reaction (pcr) for hpv using hpv l1 consensus primers and typed using l1 type-specific probes for 6/11, 16, 18 and 33. the prevalence of hpv by pcr was almost double that by vt (23.5% as compared to 12.6%, respectively). the increase was due largely to hpv types other than 6/11, 16, 18 and 33 (61.8%), w ...19957631538
cytologic observations preceding high grade squamous intraepithelial lesions.there is some evidence that a significant proportion (12%) of women, prospectively followed after negative cervical cytologic findings, develop tissue-proven high grade squamous intraepithelial neoplasia (hsil) within a short period (up to 24 months). the present study was undertaken to address the validity of this statement since such a high prevalence of spontaneous hsil, if true, may challenge the value of an annual cervicovaginal cytologic smear examination. cytologic smears collected from 8 ...19957631539
expression of the major capsid protein of human papillomavirus type 16 in escherichia coli.major capsid proteins (mcps) of various papillomaviruses have recently been expressed in heterologous cells as soluble and functional polypeptides. the host cells for producing these proteins have so far been of eukaryotic origin; however, e. coli has potential utility a host, with advantages over eukaryotic cells such as relatively simple culture requirements and greater ease of mutation of expressed sequences. we studied the expression by e. coli of the mcp of human papillomavirus type 16 (hpv ...19957635928
hpv prevalence in cytomorphologically normal cervical scrapes of pregnant women as determined by pcr: the age-related pattern.diverging data exist on human papillomavirus (hpv) prevalence in cytomorphologically normal scrapes during pregnancy. the prevalence of hpv was therefore investigated by polymerase chain reaction method (pcr) in cytomorphologically normal scrapes of 709 pregnant women and 3,948 non-pregnant women visiting the same hospital during the same time period. the prevalence of all types of hpv among pregnant women was 9.6% (68/709) and the high risk hpv types of 16 and 18 were found in 3.1% (22/709). in ...19957636509
mutational analysis of adeno-associated virus rep protein-mediated inhibition of heterologous and homologous promoters.the four rep proteins encoded by adeno-associated virus type 2 (aav-2) inhibit transcription of their own promoters and of several heterologous promoters. to gain insight into the molecular mechanism of rep-mediated transcription repression, we studied the effects of the four rep proteins on the accumulation of mrna transcribed from the human papillomavirus type 18 upstream regulatory region hpv18 urr, the human immunodeficiency virus long terminal repeat, and the aav-2 p5 and p19 promoters by t ...19957636994
efficient expression of the human papillomavirus type 16 l1 protein in epithelial cells by using rev and the rev-responsive element of human immunodeficiency virus or the cis-acting transactivation element of simian retrovirus type 1.production of the human papillomavirus (hpv) late gene products l1 and l2 is limited to terminally differentiated keratinocytes. here, we demonstrate that mrna encoding the hpv-16 l1 capsid protein contains cis-acting rna elements that inhibit expression at the posttranscriptional level. while cytoplasmic l1 mrna is detectable in transfected hela cells, l1 protein is not produced. we have identified at least one major inhibitory element that is located within the l1 open reading frame, whereas a ...19957637007
involvement of prb family in tgf beta-dependent epithelial cell hypertrophy.although renal hypertrophy is often associated with the progressive loss of renal function, the mechanism of hypertrophy is poorly understood. in both primary cultures of rabbit proximal tubules and nrk-52e cells (a renal epithelial cell line), transforming growth factor beta 1 (tgf beta) converted epidermal growth factor (egf)-induced hyperplasia into hypertrophy. tgf beta did not affect egf-induced increases in c-fos mrna abundance or cyclin e protein abundance, but inhibited egf-induced entry ...19957698989
involvement of the p53 tumor suppressor in repair of u.v.-type dna damage.the tumor suppressor p53 plays a central role in the cellular responses to genotoxic stress. besides its well known role in activation of the g1 checkpoint after exposure to agents like ionizing radiation and its role in apoptosis, the possibility exists that p53 may have additional roles, such as in dna repair. for example, p53, is known to bind to single strand dna such as would occur during repair events, and the proteins encoded by two p53-regulated genes have previously been found to bind t ...19957700629
detection of human papillomavirus dna on surgeons' gloves: possible implications for patients with cervical cancer. 19957759166
invasive squamous-cell carcinoma in giant anorectal condyloma (buschke-löwenstein tumor).giant condyloma acuminata, first described by buschke and löwenstein in 1925 as a penile lesion, is extremely rare in the anorectal region. the cauliflower-like tumor behaves clinically in a malignant fashion, although it shows no histomorphological criteria of malignancy. up to the time of writing only 33 cases of anorectal origin, 42% with malignant transformation, have been published. the authors report 2 more cases of squamous-cell carcinoma in giant anorectal condylomata acuminata. buschke- ...19957760649
sequence determination of human papillomavirus type 6a and assembly of virus-like particles in saccharomyces cerevisiae.human papillomavirus 6a (hpv6a), the most abundant hpv6 subtype, was detected in a vulvar condyloma acuminatum. the complete genome of hpv6a was cloned, and its dna sequence was shown to be over 97% identical to the hpv6b sequence. of the eight open reading frames (orfs) of hpv6a, only the imputed amino acid sequence of the major capsid protein l1 was identical to the corresponding hpv6b sequence; all other hpv6a orfs showed amino acid changes compared to the hpv6b orfs. the hpv6a l1 or the l1 + ...19957778283
human papillomavirus and widespread cutaneous carcinoma after puva photochemotherapy.oral psoralen with uv-a (puva) photochemotherapy is known to cause cutaneous malignancies and has been associated with cutaneous immunosuppression. human papillomavirus infection has also been associated with cutaneous malignancies and with immunosuppressed individuals. we therefore sought evidence of human papillomavirus infection in a patient with a long history of puva therapy and multiple cutaneous malignancies.19957778923
overexpression of bcl-xs sensitizes mcf-7 cells to chemotherapy-induced apoptosis.resistance to apoptosis plays an important role in tumors that are refractory to chemotherapy. we report that bcl-xl, which functions like bcl-2 to inhibit apoptosis, is highly expressed in mcf-7 human breast carcinoma cells. we used bcl-xs, a dominant negative inhibitor of bcl-2 and bcl-xl, to demonstrate the role of these genes in modulating chemotherapy-induced apoptosis. bcl-xs overexpressed in mcf-7 cells by stable transfection does not affect viability by itself but induces a marked increa ...19957780958
combined oral carcinogenicity of hpv-16 and benzo(a)pyrene: an in vitro multistep carcinogenesis model.we previously immortalized normal human oral keratinocytes by transfection with recombinant hpv-16 dna and subsequently exposed the cells to benzo(a)pyrene for 7 days. the exposure to benzo(a)pyrene modified the immortalized cells: the modified cells (hok-16b-bap) proliferated in an ordinary culture medium containing physiological calcium level (1.5 mm), but demonstrated only enhanced proliferation capacity without tumor formation in nude mice and failed to show in vitro anchorage-independency. ...19957784058
expression of cytochrome p450 and microsomal epoxide hydrolase in cervical and oral epithelial cells immortalized by human papillomavirus type 16 e6/e7 genes.epidemiological evidence suggests that the presence of human papillomaviruses (hpv), when combined with smoking behaviors, considerably enhances the risk of developing oral, cervical, vulvar, and/or anal carcinomas. it is well established that the cytochrome p450 (cyp), microsomal epoxide hydrolase (meh), and other biotransformation enzymes are important modulators of the bioactivation and detoxification of many environmental chemicals, including constituents of tobacco smoke such as certain nit ...19957788860
establishment and characterization of a human cell line from a squamous carcinoma of the tongue.a human squamous carcinoma cell line, pwh-s1, has been established from the metastatic lymph node of a chinese patient with a squamous cell carcinoma of the tongue. the fibroblast-free culture has been propagated in dmem supplemented with 10% fetal bovine serum for more than 100 passages. pwh-s1 cells showed anchorage-independent growth in 0.3% agar solution. pwh-s1 cell line exhibited a monolayer growth and loss of contact inhibition. the in vitro doubling time of the pwh-s1 cell line was appro ...19957788927
drug therapies for sexually transmitted diseases. clinical and economic considerations.sexually transmitted diseases (stds) are common, and result in immense social and economic costs. in some countries they have a major demographic impact. because many stds facilitate the transmission of hiv, the consequences of stds are further increasing. at the same time, this association between stds and hiv provides one of the ways in which drug therapy should be very cost effective. the perspective taken in this article is a societal one, and broader issues than those directly related to dr ...19957789285
interferons alpha, beta and gamma therapy of anogenital human papillomavirus infections.anogenital condyloma acuminatum (genital warts) is the most commonly diagnosed sexually transmitted viral disease in the united states. at least 14 of the more than 60 types of human papillomaviruses (hpvs) are responsible for condyloma acuminatum. anogenital condyloma acuminatum has a broad spectrum of manifestations in men and women, including subclinical latent infection, clinically apparent warts, abnormal genital cytology and squamous carcinoma. traditional therapeutic modalities include cy ...19957792318
establishment and characterization of a cell line (cumc-3) derived from a human squamous carcinoma of the uterine cervix.a new cell line, cumc-3, has been derived from an invasive nonkeratinizing squamous cell carcinoma of the uterine cervix in a 32-year-old patient. it has been maintained in long-term culture for 59 months, and passaged over 310 times. monolayer-cultured cells were polygonal in shape, showing a pavement-like arrangement and a tendency to pile up without contact inhibition. the epithelial nature of the cultured cumc-3 cells was also confirmed by transmission electron microscopy which demonstrated ...19957705700
human t-cell lymphotropic virus type i and severe neoplasia of the cervix in jamaica.human t-cell lymphotropic virus type i (htlv-i) was associated with carcinoma of the cervix in japan in a recent study that compared hospital cases with healthy population-based controls. to test this relationship in women more alike for cervical neoplasia risk factors (including sexual behavior and human papilloma virus; hpv), we enrolled consecutive patients from a colposcopy clinic in kingston, jamaica (an htlv-i endemic area). patients underwent pap smear, colposcopy, biopsy and cervical swa ...19957705929
human papillomavirus infection is transient in young women: a population-based cohort study.the prevalence of human papillomavirus (hpv) infection in cervical cell scrapes from a cohort of 276 young women was determined by a general two-step polymerase chain reaction. hpv infection fluctuated among young women during a 2-year interval. the total prevalence of hpv infection decreased from 21% to 8.3%. the most prevalent hpv types at enrollment were hpv-16 (3.3%) and hpv-6 (2.9%). at follow-up, the most common type was hpv-16 (2.9%), while no hpv-6 was detected. in 2 women only, the same ...19957706782
the rev protein of human immunodeficiency virus type 1 counteracts the effect of an au-rich negative element in the human papillomavirus type 1 late 3' untranslated region.we have identified a sequence in the late 3' untranslated region of human papillomavirus type 1 mrnas that acts posttranscriptionally to repress gene expression. deletion analysis localized the inhibitory element to an au-rich sequence between nucleotides 6958 and 6984 on the human papillomavirus type 1 genome. this sequence inhibits gene expression in an orientation-dependent manner. upon transfection of eucaryotic cells with plasmids containing this sequence, approximately 4-fold-lower cytopla ...19957707519
human papillomavirus not found in squamous and large cell lung carcinomas by polymerase chain reaction. 19957712451
sperm as a noninvasive gene delivery system for preimplantation embryos.to determine if sperm could be manipulated to be a noninvasive transport carrier for the delivery of gene fragments to the blastocyst.19957720929
human papillomavirus infection in human immunodeficiency virus-seropositive women.to compare the prevalence of human papillomavirus (hpv) infections in women who are seropositive and seronegative for human immunodeficiency virus (hiv), and to determine if associations between hpv and cervical disease are altered in hiv-seropositive women.19957724095
prognostic significance of human papillomavirus dna in vulvar carcinoma.to determine the histopathologic, epidemiologic, and prognostic significance of human papillomavirus (hpv) dna in primary invasive vulvar cancer.19957724101
reconstitution of p53-ubiquitinylation reactions from purified components: the role of human ubiquitin-conjugating enzyme ubc4 and e6-associated protein (e6ap).the e6 protein of the high-risk human papillomaviruses inactivates the tumor suppressor protein p53 by stimulating its ubiquitinylation and subsequent degradation. ubiquitinylation is a multistep process involving a ubiquitin-activating enzyme, one of many distinct ubiquitin-conjugating enzymes, and in certain cases, a ubiquitin ligase. in human papillomavirus-infected cells, e6 and the e6-associated protein are thought to act as a ubiquitin-protein ligase in the ubiquitinylation of p53. here we ...19957724550
an evaluation of human papillomavirus testing for intermediate- and high-risk types as triage before colposcopy.our purpose was to evaluate the role of testing for intermediate- and high-risk human papillomavirus by use of a hybrid capture technique for predicting which patients with abnormal papanicolaou smears are most likely to have squamous intraepithelial lesions or cancer.19957726250
human papillomavirus and esophageal squamous cell carcinoma. 19957729660
human papillomavirus and cervical intraepithelial neoplasia grade ii-iii: a population-based case-control study.the association between certain human papillomaviruses (hpv) and cervical intraepithelial neoplasia (cin) is well documented, but there is uncertainty about the strength of association and the role of co-factors is unclear. this population-based case-control study in norwegian women 20-44 years of age included 103 cases with histologically confirmed cin ii-iii and 234 age-matched and randomly selected controls. cytological specimens from the cervix were analyzed using the polymerase chain reacti ...19957729940
oral and laryngeal papilloma: a pediatric manifestation of sexually transmitted disease?laryngeal and oral papilloma in pediatric patients are generally considered transmitted by maternal fetal transmission. in adults and immunocompromised patients, it is well documented that human papilloma virus (hpv) of the oropharynx is sexually transmitted. the possibility of sexual transmission of oral and laryngeal papilloma in children by oral genital contact is discussed.19957729997
the human papillomavirus (hpv)-6 and hpv-16 e5 proteins co-operate with hpv-16 e7 in the transformation of primary rodent cells.e5 is the smallest transforming protein encoded by the human papillomaviruses (hpvs). it has been shown to promote anchorage-independent growth in established nih 3t3 cells, an activity that is enhanced in the presence of epidermal growth factor (egf). it is thought that this activity of e5 is brought about by an increase in the half-life of stimulated egf receptors, possibly through the perturbation of receptor processing. recent studies have also shown that e5 can co-operate with hpv-16 e7 to ...19957730808
serum antibody against unfused recombinant e7 protein of human papillomavirus type 16 in cervical cancer patients.sera were examined for the presence of antibody against e7 protein of human papillomavirus type 16 (hpv-16) by western blot analysis using the bacterially derived unfused protein. the occurrence rates of anti-e7 antibody against hpv-16 were 14.1% (10/71) in cervical cancer patients, 0% (0/48) in cervical intraepithelial neoplasia patients, and 0% (0/41) in female non-malignant patients. three patients (one with endometrial cancer, one with breast cancer, and one male patient with colon polyp) ou ...19957737906
pathology and management of vulval pain and pruritus.vulval pain and pruritus are symptoms associated with benign or malignant diseases. the paucity of information regarding aetiological factors confounds a logical approach to successful therapeutic and preventative strategies. research continues to address the possible relationship between human papillomavirus and benign, premalignant and malignant vulval conditions. although present in many conditions, oncogenesis caused by human papillomavirus has yet to be proven as a cause of vulvar premalign ...19957742509
binding and internalization of human papillomavirus type 33 virus-like particles by eukaryotic cells.infection of cells by human papillomaviruses (hpvs) associated with malignant genital lesions has not been studied because of the lack of an in vitro system and the unavailability of virions. we have now used virus-like particles (vlps) of hpv type 33 to analyze the initial events in the interaction of the hpv capsid with cell lines. binding of vlps to hela cells was observed in biochemical assays and by immunofluorescence. vlp binding was inhibited by antisera raised against vlps but not by mon ...19957745672
human papillomavirus type 31b late gene expression is regulated through protein kinase c-mediated changes in rna processing.expression of the human papillomavirus (hpv) capsid genes, l1 and l2, as well as amplification of viral dna and virion assembly occur in the terminally differentiated layers of infected stratified squamous epithelium in vivo. these processes can be duplicated in the laboratory through the use of organotypic or raft cultures. when cin612 cells, which contain episomal copies of the high-risk hpv type 31b, are allowed to differentiate in raft cultures, the expression of transcripts encoding the ear ...19957745684
oligomerisation of full length p53 contributes to the interaction with mdm2 but not hpv e6.the tumour suppressor protein p53 normally functions as a tetramer in a defined conformational state. mutations within p53 which contribute to cancer development frequently induce a conformational shift in the protein which correlates with loss of wild type growth suppressor functions. both the cell encoded mdm2 protein and the human papillomavirus oncoprotein e6 can regulate p53 function and we have examined the interaction of these proteins with p53. the e6/p53 association is sensitive to conf ...19957753547
differentiated carcinomas develop as a consequence of the thyroid specific expression of a thyroglobulin-human papillomavirus type 16 e7 transgene.the oncogenic properties of the high risk human papillomaviruses (hpv) e7 protein are attributed to its interaction with the retinoblastoma susceptibility gene product rb1 and other related proteins. we report here the generation of a transgenic model expressing the e7 oncogene of hpv16 in thyroid follicular cells, under control of the bovine thyroglobulin gene promoter. transgenics develop differentiated and functionally regulated thyroid goitres, due to thyroid cell proliferation and accumulat ...19957753555
genetic characterization of the human papillomavirus (hpv) 18 e2 gene in clinical specimens suggests the presence of a subtype with decreased oncogenic potential.hpv 18 is associated with 2 divergent phenotypes: (i) aggressive cervical cancer and a preponderance of cancer relative to cervical intra-epithelial neoplasia (cin) and (ii) benign warty lesions of the cervix. the e2 gene of hpv 18 encodes a regulatory protein that represses viral oncogene transcription and is involved in viral replication. variation within the e2 gene of hpv 18 and its correlation with the morphologic grade of associated lesions were analyzed in a sample of 20 hpv 18-positive c ...19957829247
association of human leukocyte antigen-b1*03 with cervical cancer in japanese women aged 35 years and younger.an association of human leukocyte antigen (hla)-dqw3 alleles with squamous cell carcinoma (scc) of the cervix has been reported in some european populations, but the significance of hla-dqw3 has not been examined in other populations to the authors' knowledge. the interaction between hla-dqw3 and human papillomavirus (hpv) in scc remains to be clarified.19957812922
cis-acting components of human papillomavirus (hpv) dna replication: linker substitution analysis of the hpv type 11 origin.papillomavirus dna replication requires the viral trans-acting factors e1 and e2 in addition to the host cell's general replication machinery. the origins of dna replication in bovine and human papillomavirus genomes have been localized to a specific part of the upstream regulatory region (urr) which includes recognition sites for e1 and e2 proteins. to fine map cis-acting elements influencing human papillomavirus type 11 (hpv-11) dna replication and to determine the relative contributions of su ...19957815528
simultaneous detection by consensus multiplex pcr of high- and low-risk and other types of human papilloma virus in clinical samples.a consensus multiplex pcr (cm-pcr) technique was developed to detect high-risk (hpv 16/18), low-risk (hpv 6/11), and over 40 other types of human papillomavirus (hpv), separately but simultaneously, by mixing three pairs of consensus primers in the same pcr mixture, for gene amplification. simultaneous detection of three groups of hpv dna provides valuable information for clinical practice and this procedure is simple and convenient for routine laboratory examinations. we detected hpv dna sequen ...19957622102
can a test for e6/e7 transcripts of human papillomavirus type 16 serve as a diagnostic tool for the detection of micrometastasis in cervical cancer?tissue from 11 cases of cervical cancer positive for human papillomavirus (hpv) type 16 dna and 69 pelvic lymph nodes from the same patients were examined for hpv 16 dna and mrna from the e6/e7 genes. five of the tumors were squamous, 3 adeno- and 3 adenosquamous carcinoma. from the primary tumors and the extirpated lymph nodes dna and rna or mrna was subjected to pcr and rt-pcr. three transcription profiles (only e6*i, e6*i and e6*ii or full-length e6-e7 plus both of the spliced transcripts) we ...19957622311
interaction of papillomavirus e6 oncoproteins with a putative calcium-binding protein.human papillomaviruses (hpvs) are associated with the majority of cervical cancers and encode a transforming protein, e6, that interacts with the tumor suppressor protein p53. because e6 has p53-independent transforming activity, the yeast two-hybrid system was used to search for other e6-binding proteins. one such protein, e6bp, interacted with cancer-associated hpv e6 and with bovine papillomavirus type 1 (bpv-1) e6. the transforming activity of bpv-1 e6 mutants correlated with their e6bp-bind ...19957624774
prevalence of human papillomavirus among std clinic attenders in jamaica: association of younger age and increased sexual activity.human papillomavirus is the major etiologic agent of cervical cancer. although the incidence of cancer of the cervix is high in jamaica, the prevalence of human papillomavirus among jamaican women has not been defined.19957624812
rare association of human papillomavirus dna with esophageal cancer in japan.to examine whether human papillomavirus (hpv) dna is associated with esophageal cancer, frozen and paraffin-embedded neoplasms of the upper aerodigestive tract, including esophageal cancer, were investigated. dna obtained from frozen specimens and cell lines were analyzed by both polymerase chain reaction (pcr) and southern blot hybridization. dna from paraffin-embedded samples were analyzed strictly by pcr. dna of hpv types 6 and 11 was detected in papillomas of the upper respiratory tract at > ...19957844381
binding of the lxcxe insulin motif to a hexapeptide derived from retinoblastoma protein.peptides corresponding to retinoblastoma protein (rb) fragment 649-654 (lfykkv) were tested for their ability to recognize the lxcxe sequence motif in human papilloma virus type 16e7 protein (hpv-16e7) encompassing e7 residues 21-26 (dlycye) and an identical motif in human insulin comprising insulin b-chain residues 16-21 (ylvcge), respectively. interaction between these complementary peptide sequences was observed by several approaches, including direct and competitive elisa as well as affinity ...19957818556
detection of human papillomavirus dna in colorectal adenomas.to assess the presence of different types of human papillomavirus (hpv) dna in colorectal adenomas.19957802580
vulvar intraepithelial neoplasia: age, morphological phenotype, papillomavirus dna, and coexisting invasive carcinoma.recent studies suggest that subsets of vulvar intraepithelial neoplasia (vin) may be distinguished based on morphological presentation, the presence or absence of human papillomavirus (hpv) nucleic acids, and patient age. we analyzed 65 vin lesions, including 15 with associated squamous cell carcinoma, to determine the relationship between pathological parameters associated with common types of vin (multinucleation, koilocytosis, verruco-papillary morphology, diffuse atypia), rarer variants (dif ...19957860044
risk of acquiring human papillomavirus from the plume produced by the carbon dioxide laser in the treatment of warts.the documented presence of human papillomavirus dna in the plume after carbon dioxide laser treatment of warts has raised questions about the risk of transmission of human papillomavirus to laser surgeons.19957868712
functional p53 protein in human papillomavirus-positive cancer cells.there is accumulating evidence that the p53 protein contributes to tumor suppression by stimulating the transcription of specific cellular genes, such as the cell cycle control gene waf1/clp1. p53-mediated transcriptional activation is inhibited in cotransfection assays by overexpressed e6 protein from cancer-associated human papillomavirus (hpv) types, pointing at a possible molecular mechanism by which these viruses contribute to malignant cell transformation. here we analysed the transcriptio ...19957898934
absence of human papillomavirus in squamous cell carcinomas of nongenital skin from immunocompromised renal transplant patients: a comment. 19957880308
inhibition of growth of normal and human papillomavirus-transformed keratinocytes in monolayer and organotypic cultures by interferon-gamma and tumor necrosis factor-alpha.the growth response of normal and human papillomavirus (hpv)-transformed cervical keratinocytes to interferon-gamma (ifn-gamma) and tumor necrosis factor-alpha was investigated in monolayer and organotypic raft cultures. the proliferation rates of monolayer cultures were assessed by [3h]tdr incorporation and fluorimetric dna titration. the growth of keratinocytes in organotypic cultures was estimated by their ability to stratify on collagen rafts and by immunohistochemistry for ki67 antigen expr ...19957887441
human papillomavirus testing by hybrid capture appears to be useful in triaging women with a cytologic diagnosis of atypical squamous cells of undetermined significance.our purpose was to determine the clinical value of human papillomavirus deoxyribonucleic acid testing with the hybrid capture test, specifically to examine whether human papillomavirus testing could identify which women with papanicolaou smears read as atypical squamous cells of undetermined significance were most likely to have histologically confirmed cervical intraepithelial neoplasia.19957892889
polymorphism of the hpv-16 e6 gene of cervical-carcinoma.polymorphism of the human papillomavirus type 16 (hpv-16) genome has been reported to occur within the noncoding regulatory long control region (lcr) and in the e7 and l1 genes. the current study focuses on the hpv-16 e6 oncogene which interacts with the antioncogenic regulator p53. seventy-eight hpv-16-positive dna samples derived from cervical carcinomas were screened for the presence of polymorphism in the hpv-16 e6 gene by polymerase chain reaction (pcr) linked single stranded conformational ...199521552834
the gene encoding the p53-regulated inhibitor of cdks (pic1) is not expressed in the molt-4 leukemia-cell line with p53 truncated at the carboxyl-terminus, and harbors a nucleotide substitution at codon-31 in several other cancer cell-lines.entry into the cell cycle is governed by cyclins, cyclin-dependent kinases (cdks) and cdk-inhibitors (cdkis). the p53-regulated inhibitor of cdks (pic1) is a universal cdki whose gene expression is directly induced by the p53 tumor suppressor protein. reverse transcription and polymerase chain reaction revealed strong pic1 gene expression in control mrc-5 human embryo lung cells, but relatively weaker bands in a549 lung carcinoma; hep3b, mahlavu, plc/prf/5 hepatocellular carcinoma; siha, caski, ...199521556614
evaluation of two commercially available dna tests for detection of human papillomavirus.this study was designed to compare the sensitivity, specificity, efficiency, positive and negative predictive values, and ease of use for 2 commercially available hybridization kits for detecting human papillomavirus (hpv) dna: oncor southern blot (sb) (oncor, inc., gaithersburg, md) and digene viratype dot blot (db) (digene diagnostics, inc., silver spring, md).199518475405
sexually transmitted and other genital infections in women with cervical human papillomavirus infection.we investigated possible correlations between latent cervical human papillomavirus infection (chpi) and other sexually transmitted diseases (stds).199518476023
isolation of dna from archival papanicolaou stained cytological smears using a simple salting-out procedure.dna from archival papanicolaou stained and unstained cytological smears was successfully isolated using a simple, rapid and inexpensive salting-out procedure. the quality of dna was controlled by polymerase chain reaction (pcr) amplification of segments of the human beta-globin, human beta-actin and human papillomavirus l1 genes. only negligible differences in amplification efficiency were observed between dna isolated from stained and unstained smears. the salting-out procedure is a more rapid ...199516695977
regulation of human papillomavirus transcription by the differentiation-dependent epithelial factor epoc-1/skn-1a.human papillomavirus (hpv) early gene expression is closely linked to the differentiation status of infected epithelial cells. typically, hpv type 16 (hpv16) or hpv18 e6 and e7 transcripts are only barely detectable within the undifferentiated basal cell layer, but their levels increase concomitantly with higher degrees of epithelial cell differentiation in suprabasal cells. a similar differentiation-dependent distribution of expression has been reported for the recently cloned epithelial cell s ...19968523512
comparison of the cervical cytology test using the papnet method and conventional microscopy.from august 1994 to june 1995, laboratories in 28 mexican states and the federal district submitted a total of 10098 diagnosed pap test slides to mexico's national institute of epidemiologic diagnosis and reference (indre) for reexamination by conventional methods and also by the automated papnet system in suffern, new york, u.s.a. the aim was to determine the degree of agreement obtained by these various methods. most of the slides examined (at least 78%) yielded negative results or merely indi ...19969041745
the causal link between hpv and cervical cancer and its implications for prevention of cervical cancer.this article reviews epidemiologic evidence linking human papillomavirus (hpv) to cervical cancer. the authors conclude that over 90% of all cervical cancers can be attributed to certain hpv types-hpv 16 accounting for the largest proportion (roughly 50%) followed by hpv 18 (12%), hpv 45 (8%), and hpv 31 (5%). recognition of this circumstance has far-reaching implications for primary and secondary prevention of this malignancy. at present, prophylactic and therapeutic hpv vaccines are under deve ...19969041748
workshop on screening for cancer of the uterine cervix in central america.this article describes the objectives and content of a workshop held in managua, nicaragua, during november 1995, on screening for cervical cancer. the aims were to discuss cost-effective models of screening in countries with a high incidence of cervical cancer and to reach a consensus on principles for screening that is balanced with a country's resources. the workshop aimed to develop a planning framework and to identify program strengths and weaknesses by country. in 1990 there were 25,000 ...19969041753
national institutes of health consensus development conference statement: cervical cancer, april 1-3, 1996. national institutes of health consensus development panel.the objective was to provide physicians and the general public with a responsible assessment of current screening, prevention, and treatment approaches to cervical cancer.19969023821
cervical human papillomavirus infection and intraepithelial neoplasia: a review.cervical human papillomavirus (hpv) infections and intraepithelial neoplasias are precursors to cervical cancer, the second most common cancer in women worldwide. hpv satisfies the epidemiologic criteria for causality; the role of other cofactors is under study. natural history studies show that most low-grade lesions (productive hpv infections) regress or persist, whereas high-grade lesions (those with integrated hpv dna) progress. immunobiologic studies demonstrate that infection peaks in the ...19969023824
human papillomavirus biology. 19969023844
detection of human viruses in periodontal pockets using polymerase chain reaction.even though viruses have been implicated in the etiology of several medical and dental disorders, little or no data are available on the possible involvement of human viruses in the pathogenesis of human periodontal disease. this study investigated the presence of human cytomegalovirus, epstein-barr virus, herpes simplex virus, human papillomavirus and human immunodeficiency virus (hiv) in crevicular fluid samples from 30 patients with advanced periodontitis and 26 subjects with gingivitis. vira ...19969028252
hybrid capture method for detection of human papillomavirus dna in clinical specimens: a tool for clinical management of equivocal pap smears and for population screening.high- and intermediate-risk types of human papillomavirus (hpv) in concert with cofactors are responsible for over 90% of cervical cancers world-wide. while the pap smear is a valuable cancer prevention tool, its subjective nature leaves it error prone. as a result, hpv dna testing appears to be a needed adjunct to the pap smear. hybrid capture is a simple dna test with high specificity and sensitivity which appears useful for clinical management of equivocal pap tests and shows promise for mass ...19969037955
intra-epithelial and invasive cervical neoplasia during hiv infection.patients affected by human immunodeficiency virus (hiv) infection present an elevated risk of developing cancer. in the last 10 years, the relationship between human papilloma virus (hpv) infection and female cervical intra-epithelial neoplasia (cin) has been established. several studies have described an increased prevalence of both cervical hpv infection and cin among hiv-positive women compared to hiv-negative ones. a high recurrence rate of cin after standard treatment has been noted in hiv- ...19969038602
improved detection of human papillomavirus infection in genital intraepithelial neoplasia in human immunodeficiency virus positive (hiv +) women by polymerase chain reaction-in situ hybridization.the prevalence of genital human papillomavirus (hpv) infection was evaluated in 30 consecutive human immunodeficiency virus (hiv) + women by polymerase chain reaction (pcr)-in situ hybridization (ish) on paraffin-embedded tissue sections and compared with that found with standard ish. biopsies were removed from normal or neoplastic areas in the cervix, vagina, and vulva, and ish was performed with biotinylated or fluorescein isothiocyanate genomic dna probes. one probe was used for hpv screening ...19968727101
hiv-1, hiv-2, human papillomavirus infection and cervical neoplasia in high-risk african women.to determine the effect of hiv-1 and hiv-2 infection on the prevalence of cervical human papillomavirus (hpv) and squamous intraepithelial lesions (sil) in a population of high-risk women in senegal.19968728046
in situ hybridization study on human papillomavirus dna expression in benign and malignant squamous lesions of the esophagus.histologic changes suggesting hpv infection are occasionally found adjacent to squamous cell carcinoma or in squamous papilloma of the esophagus, but the relationship between hpv infection and benign and malignant squamous lesions of the esophagus is not yet dear. the aim of this study was to examine the role of hpv in squamous lesions of the esophagus. microscopic examination with emphasis on hpv infection was done on 15 cases of squamous cell carcinoma and 26 cases of squamous papilloma. in si ...19969008094
properties of hpv-positive and hpv-negative anal carcinomas.evidence of human papillomavirus (hpv) can be found in up to 85 per cent of anal carcinomas. in the vulva, a discrete subset of hpv-positive carcinomas which show koilocytic morphology and distinct clinical features has recently been identified (warty carcinoma). the morphological and prognostic features of hpv-positive and hpv-negative anal carcinomas were compared in this study of the tumour distribution of hpv dna. vulval and anal neoplasia are similar in many ways and we have also looked to ...19969014857
epstein-barr virus nuclear antigen (ebna)3c is an immortalizing oncoprotein with similar properties to adenovirus e1a and papillomavirus e7.epstein-barr virus (ebv) requires six genes to efficiently immortalize human b cells. we have shown that one of these, ebna3c, can cooperate with activated (ha-)ras in co-transfection assays to immortalize and transform rat embryo fibroblasts (refs). ebna3c also augmented transformation by (ha-)ras and a mutant p53 to a similar extent as human papilloma virus e7. as with e7 this effect was not inhibited by cotransfection with the cyclin-dependent kinase inhibitor (cdki), a p16ink4a, which can no ...19969000128
presence of human papillomavirus type 6f in tonsillar condyloma acuminatum and clinically normal tonsillar mucosa.an attempt was made to detect human papillomavirus (hpv) in 8 cases of oral papilloma by southern blot hybridization. hpv type 6 was identified in a case of tonsillar condyloma acuminatum, but neither hpv type 6 nor 11 was found in the 7 other cases of squamous papilloma. the hybridization pattern of the hpv type 6 dna after digestion with restriction enzymes revealed that the hpv was of the 6f subtype. dna samples isolated from the condyloma acuminatum, 3 peripheral specimens of clinically norm ...19969001342
infection of human papillomavirus (hpv) and p53 over-expression in human female genital tract carcinoma.inactivation of p53 gene products either by mutation or by complex formation with e6 oncoprotein encoded by high risk hpv appears to be a common event in cervical carcinogenesis. this study was designed to clarify this association in 41 primary cervical, 15 endometrial, 3 ovarian and one rectal carcinomas. polymerase chain reaction analysis revealed presence of high risk hpv in 36 (88%) cervical, 5 (33%) endometrial and none of ovarian and rectal carcinomas. hpv 16 was found in 14 cervical carci ...19968961706
[human papilloma virus. epidemiologic, carcinogenic, diagnostic and therapeutic aspects].human papillomavirus (hpv) is the most common infection among women. natural history of hpv infection has changed as well as diagnosis and treatment. clinical picture is variable even with spontaneous remission. molecular biology for diagnosis and typification of hpv has changed its treatment. the disease can be treated according to clinical experience. sometimes can not eradicated since just it is impossible make to disappear it from genital tract.19968964543
p53-null cells are more sensitive to ultraviolet light only in the presence of caffeine.we have shown previously that p53(-/-) fibroblasts show greater sensitization by caffeine to the lethal effects of ionizing radiation compared with p53(+/+) cells. recently published data have suggested a possible role of p53 in nucleotide excision repair: an association of p53 and xeroderma pigmentosum group b protein and a greater sensitivity to cisplatin of rko cells transfected with the e6 protein of human papilloma virus (inactivating p53). we show that p53(+/+) and p53(-/-) cells have equa ...19968968086
a neglected lesbian health concern: cervical neoplasia.many health care providers believe that lesbian women do not need routine papanicolaou smears. this erroneous assumption fails to recognize that the majority of lesbian women have had prior heterosexual relationships and thus may be at risk for developing cervical neoplasia. the following case report describes a lesbian woman who denied prior heterosexual intercourse but who developed cervical intraepithelial neoplasia grade 2 (cin 2) associated with a high oncogenic risk human papillomavirus (h ...19968969707
e5 proteins of human papillomavirus types 11 and 16 transactivate the c-fos promoter through the nf1 binding element.human papillomavirus type 11 (hpv-11) and hpv-16 contain an e5 gene that can induce c-fos gene expression in mouse fibroblasts. this study investigated the human c-fos promoter characteristics by mapping the c-fos promoter sequence with several deletion and point mutants that confer responsiveness to e5 of hpv-11 or hpv-16. the mutant studies show that nf1 binding sequences within the c-fos promoter were crucial for the induction of the c-fos gene by e5, and the gel shift assay study suggested t ...19968970980
human papillomavirus vaccines. 19968971215
expression of the major capsid protein of human papillomavirus type 11 in saccharomyces cerevisae.the major capsid protein l1 of papillomaviruses expressed recombinantly or in infected cells has the intrinsic ability to form virus-like particles (vlps) which display conformational epitopes necessary to elicit high-titered, virus-neutralizing antibodies. we have shown previously that the l1 gene of human papillomavirus type 6a (hpv6) can be expressed efficiently in saccharomyces cerevisae (sc) as vlps. however, when we attempted to express the l1 gene cloned from the closely related hpv11 in ...19968973339
the cervico-vaginal epithelium during 20 cycles' use of a combined contraceptive vaginal ring.the aim of the study was to evaluate the influence of a combined contraceptive vaginal ring (ccvr) made of silastic on the cervico-vaginal epithelium during 20 cycles of use. a total of 76 volunteers used the ccvr releasing 0.120 mg etonogestrel and 0.015 mg ethinyloestradiol daily. cytological samples were taken of the vaginal epithelium, the ectocervix and the endocervix before the start, at 4 and 12 months, and at the end of the study. cytology, hormonal profiles, human papilloma virus (hpv) ...19968981130
genital viral infections. studies on human papillomavirus and epstein-barr virus.the concept 'sexually transmitted diseases' (std) was introduced in the nineteen sixties and comprises many diseases of varying importance from scabies to hiv infection. the std family has grown wider and new members will probably join. the starting point of these studies was that patients with hpv infection, attending the std clinic, appeared to increase in number in the late eighties. hpv was analysed with the southern blot technique from portio cervix in a female population attending the std ...19969111847
papillomaviruses and potential copathogens.an in vitro multistage genital epithelial cell model for cervical cancer that parallels the in vivo neoplastic process has been developed using recombinant human papillomavirus (hpv) dna and genital cells. hpv-16-immortalized genital cells are responsive to the genotoxic action of known chemical carcinogens (polycyclic hydrocarbons, alkylating agents or cigarette smoke condensate), but are not converted to malignancy. ras oncogene and human herpes virus-2 did convert hpv immortalized cells to ma ...19968920709
up-regulation of hormone response of human papillomavirus type 16 expression and increased dna-protein binding by consensus mutations of viral glucocorticoid response elements.human papillomaviruses (hpvs) and steroid hormones are linked to the development of cervical cancer. studies from our laboratory and others showed that the steroid glucocorticoid and progesterone hormones activated the expression of hpv type 16. this activation was attributed to the specific interaction of the glucocorticoid receptor (gr) with the three glucocorticoid response elements (gres) in the hpv16 regulatory region. in the present study, we first examined the glucocorticoid response medi ...19968923291
[retrospective study of cervical papillomavirus lesions: early herpes simplex virus proteins as markers of risk for progression].specific types of human papillomavirus (hpv) are currently implicated as etiologic agent of benign and malignant lesions of the genital tract. previous studies highlighted the presence of early proteins (icsp 11/12) of herpes simplex virus type 2 (hsv-2) mainly in hpv infected lesions of the cervix. this paper reports a retrospective study on the clinical course of benign cervical alterations in a 70 women population followed-up for 24 months. hpv and hsv-2 (late and early) antigens were detecte ...19968927276
Refresh
firstprev
...11121314151617181920...
nextlast
Displaying items 1401 - 1500 of 6259