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precopulatory acoustic interactions of the new world malaria vector anopheles albimanus (diptera: culicidae).anopheles albimanus is a malaria vector in central america, northern south america and the caribbean. although a public health threat, an. albimanus precopulatory mating behaviors are unknown. acoustics play important roles in mosquito communication, where flight tones allow males to detect and attract potential mates. the importance of sound in precopulatory interactions has been demonstrated in toxorhynchites brevipalpis, aedes aegypti, culex quinquefasciatus and anopheles gambiae; convergence ...201931370863
experimental assessment of zika virus mechanical transmission by aedes aegypti.the pandemic emergence of several mosquito-borne viruses highlights the need to understand the different ways in which they can be transmitted by vectors to human hosts. in this study, we evaluated the propensity of aedes aegypti to transmit mechanically zika virus (zikv) using an experimental design. mosquitoes were allowed to feed on zikv-infected blood and were then rapidly transferred to feed on zikv-free blood until they finished their meal. the uninfected blood meals, the mosquito abdomens ...201931370135
infection and transmission of cache valley virus by aedes albopictus and aedes aegypti mosquitoes.cache valley virus (cvv; bunyavirales, peribunyaviridae) is a mosquito-borne arbovirus endemic in north america. although severe diseases are mainly observed in pregnant ruminants, cvv has also been recognized as a zoonotic pathogen that can cause fatal encephalitis in humans. human exposures to cvv and its related subtypes occur frequently under different ecological conditions in the new world; however, neurotropic disease is rarely reported. high prevalence rates of neutralizing antibodies hav ...201931366369
larvicidal activity of essential oil from vitex negundo and vitex trifolia on dengue vector mosquito aedes aegypti.the ability of vitex trifolia and vitex negundo essential oils to kill aedes aegypti and culex quinquefasciatus larvae was evaluated.201931365621
spatial diffusion of the 2015-2016 zika, dengue and chikungunya epidemics in rio de janeiro municipality, brazil.different countries, especially brazil, that have faced recurrent dengue epidemics for decades and chikungunya epidemics since 2014, have had to restructure their health services to combat a triple epidemic of arboviruses - zika, dengue and chikungunya - transmitted by the same vector, mainly aedes aegypti, in 2015-2016. several efforts have been made to better understand these three arboviruses. spatial analysis plays an important role in the knowledge of disease dynamics. the knowledge of the ...201931364556
no evidence of zika, dengue, or chikungunya virus infection in field-caught mosquitoes from the recife metropolitan region, brazil, 2015.background: the recife metropolitan region (rmr), north-eastern brazil, was the epicentre of the 2015 zika virus (zikv) epidemic, which was followed by a 2016 chikungunya virus (chikv) epidemic. it historically has amongst the highest incidence of dengue virus (denv) infections and is the only remaining focus of lymphatic filariasis (lf) in brazil. in early 2015, a molecular xenomonitoring surveillance project focused on culex (cx.) quinquefasciatus commenced to inform lf elimination activities. ...201931363498
the ecological and epidemiological consequences of reproductive interference between the vectors aedes aegypti and aedes albopictus.vector ecology is integral to understanding the transmission of vector-borne diseases, with processes such as reproduction and competition pivotal in determining vector presence and abundance. the arbovirus vectors aedes aegypti and aedes albopictus compete as larvae, but this mechanism is insufficient to explain patterns of coexistence and exclusion. inviable interspecies matings-known as reproductive interference-is another candidate mechanism. here, we analyse mathematical models of mosquito ...201931362626
the challenge of invasive mosquito vectors in the u.k. during 2016-2018: a summary of the surveillance and control of aedes albopictus.mosquito-borne diseases resulting from the expansion of two key vectors, aedes aegypti and aedes albopictus (diptera: culicidae), continue to challenge whole regions and continents around the globe. in recent years there have been human cases of disease associated with chikungunya, dengue and zika viruses. in europe, the expansion of ae. albopictus has resulted in local transmission of chikungunya and dengue viruses. this paper considers the risk that ae. aegypti and ae. albopictus represent for ...201931361038
comparative evaluation of an improved test method for bioefficacy of insecticidal fabrics against dengue and malaria vectors.insecticidal fabrics are important personal protective measures against mosquitoes, ticks and other disease vectors. in the absence of internationally accepted guidelines, bioefficacy tests have been carried out using continuous exposure and three minutes exposure bioassay methods. recently, we have reported an improved method for bioefficacy testing of insecticidal fabrics, which involves continuous exposure of mosquitoes to the test fabrics. the present paper reports the comparative evaluation ...201931358045
serological data shows low levels of chikungunya exposure in senegalese nomadic pastoralists.the chikungunya virus (chikv) is spread by aedes aegypti and ae. albopictus mosquitos worldwide; infection can lead to disease including joint pain, fever, and rash, with some convalescent persons experiencing chronic symptoms. historically, chikv transmission has occurred in africa and asia, but recent outbreaks have taken place in europe, indonesia, and the americas. from september to october 2014, a survey was undertaken with nomadic pastoralists residing in the northeast departments of seneg ...201931357631
larvicidal and enzymatic inhibition effects of annona muricata seed extract and main constituent annonacin against aedes aegypti and aedes albopictus (diptera: culicidae).the mosquitoes aedes aegypti and aedes albopictus are vectors of arboviruses that cause dengue, zika and chikungunya. bioactive compounds from plants are environmentally sustainable alternatives to control these vectors and thus the arboviruses transmitted by them. the present study evaluated the larvicidal activity of an acetogenin-rich fraction (acerf) and its main constituent annonacin obtained from annona muricata seeds on ae. aegypti and ae. albopictus. the larvicidal assays were performed ...201931357557
mosquito-borne viruses and insect-specific viruses revealed in field-collected mosquitoes by a monitoring tool adapted from a microbial detection array.several mosquito-borne diseases affecting humans are emerging or reemerging in the united states. the early detection of pathogens in mosquito populations is essential to prevent and control the spread of these diseases. in this study, we tested the potential applicability of the lawrence livermore microbial detection array (llmda) to enhance biosurveillance by detecting microbes present in aedes aegypti, aedes albopictus, and culex mosquitoes, which are major vector species globally, including ...201931350319
in vitro and in vivo toxicity assessment of selenium nanoparticles with significant larvicidal and bacteriostatic properties.in the present study, we investigated the larvicidal and bacteriostatic activity of biosynthesized selenium nanoparticles using aqueous berry extract of murraya koenigii (mk-se nps). the synthesized mk-se nps were characterized using uv-visible spectroscopy, x-ray diffraction (xrd), fourier transform infrared (ftir) spectroscopy, transmission electron microscopy (tem), scanning electron microscopy (sem) and energy dispersive x-ray (edx) analysis. xrd analysis revealed the crystalline nature of m ...201931349432
odorant inhibition in mosquito olfaction.how chemical signals are integrated at the peripheral sensory system of insects is still an enigma. here we show that when coexpressed with orco in xenopus oocytes, an odorant receptor from the southern house mosquito, cquior32, generated inward (regular) currents when challenged with cyclohexanone and methyl salicylate, whereas eucalyptol and fenchone elicited inhibitory (upward) currents. responses of cquior32-cquiorco-expressing oocytes to odorants were reduced in a dose-dependent fashion by ...201931349189
effect of chlorine and temperature on larvicidal activity of cuban bacillus thuringiensis isolates.the efficacy of biolarvicides may be influenced by species of mosquito, larval age and density, temperature, water quality, bacterial formulation, and others. the aim of this study was to evaluate the influence of temperature and chlorine on larvicidal activity of bacillus thuringiensis cuban isolates against aedes aegypti.201931346534
monitoring indole alkaloid production by penicillium digitatum during infection process in citrus by mass spectrometry imaging and molecular networking.green mold, caused by penicillium digitatum, is the most destructive post-harvest disease in citrus. secondary metabolites produced by fungal phytopathogens have been associated with toxicity to their respective host through the interaction with a wide range of cell targets. natural products have also been described as important molecules for biocontrol and competition in their respective environment. for p. digitatum, the production of indole alkaloids, tryptoquialanines a and b, have been repo ...201931345413
revisiting alkali metals as a tool to characterize patterns of mosquito dispersal and oviposition.mark-recapture methods constitute a set of classical ecological tools that are used to collect information on species dispersal and population size. these methods have advanced knowledge in disparate scientific fields, from conservation biology to pest control. gathering information on the dispersal of mosquito species, such as aedes aegypti, has become critical since the recognition of their role as vectors of pathogens. here, we evaluate a method to mark mosquitoes that exploits the rare alkal ...201931344901
autocidal gravid ovitraps protect humans from chikungunya virus infection by reducing aedes aegypti mosquito populations.public health responses to outbreaks of dengue, chikungunya, and zika virus have been stymied by the inability to control the primary vector, aedes aegypti mosquitos. consequently, the need for novel approaches to aedes vector control is urgent. placement of three autocidal gravid ovitraps (ago traps) in ~85% of homes in a community was previously shown to sustainably reduce the density of female ae. aegypti by >80%. following the introduction of chikungunya virus (chikv) to puerto rico, we cond ...201931344040
detection of wolbachia in field-collected aedes aegypti mosquitoes in metropolitan manila, philippines.recent reports reveal the presence of wolbachia in ae. aegypti. our study presents additional support for wolbachia infection in ae. aegypti by screening field-collected adult mosquitoes using two wolbachia-specific molecular makers.201931340862
corrigendum to: "screening aedes aegypti (diptera: culicidae) populations from pernambuco, brazil for resistance to temephos, diflubenzuron, and cypermethrin and characterization of potential resistance mechanisms". 201931340382
correlation between cephalic circumference at birth and ocular alterations in patients with microcephaly potentially associated with zika virus infection.the zika virus (zikv) is an arbovirus isolated for the first time in 1947 and transmitted to humans by the aedes aegypti mosquito. in brasil, it was first detected in may 2015. since then, zikv has been identified as the etiological agent of acute exanthematous disease in brasil, and neuropediatricians of the recife warned about an epidemic of microcephaly, and the brazilian ministry of health confirmed the association between zikv and congenital malformations and neurological syndromes. the eye ...201931340324
efficacy of heterorhabdits indica lpp35 against aedes aegypti in domiciliary oviposition sites.entomopathogenic nematodes have been evaluated for control of mosquito species for decades. depending on the nematode and mosquito involved, mortality rates of larvae (l) may reach 100% in vitro. nonetheless, nematode efficacy at oviposition sites has rarely been assessed. heterorhabditis indica lpp35 has been shown to kill over 75% of aedes aegypti l3/l4 in cups and bottles outdoors. to assess its efficacy in indoor oviposition sites, different types/sizes of floor drains and pot saucers, and 6 ...201931339672
ctlga9 interacts with alp1 and apn receptors to modulate cry11aa toxicity in aedes aegypti.the mosquito aedes aegypti is associated with the spread of many viral diseases in humans, including dengue virus (denvs), yellow fever virus (yfv), zika virus (zikv), and chikungunya virus (chikv). bacillus thuringiensis (bt) is widely used as a biopesticide, which produces cry toxins for mosquito control. the cry toxins bind mainly to important receptors, including alkaline phosphatase (alp) and aminopeptidase-n (apn). this work investigated the function of a c-type lectin, ctlga9, in a. aegyp ...201931339308
age- and sex-related abc transporter expression in pyrethroid-susceptible and -resistant aedes aegypti.resistance mechanisms to synthetic insecticides often include point mutations and increased expression of genes encoding detoxification enzymes. since pyrethroids are the main adulticides used against aedes aegypti, which vectors pathogens such as zika virus, understanding resistance to this insecticide class is of significant relevance. we focused on adenosine triphosphate (atp)-binding cassette (abc) transporters in the pyrethroid-resistant puerto rico (pr) strain of ae. aegypti. we investigat ...201931862982
leptolegnia chapmanii como alternativa biológica para el control de aedes aegyptileptolegnia chapmanii is a facultative pathogen of many species of mosquitoes, among which species of the genus aedes, culex and anopheles stand out for their medical and sanitary importance. the potential of l. chapmanii as an alternative to control lies in its virulence, pathogenicity and specificity against the larval stages of mosquitoes, and because of its harmlessness to non-target species such as fish and amphibians, among others. the natural presence of l. chapmanii had been reported in ...201931860189
the first characterization of a cystatin and a cathepsin l-like peptidase from aedes aegypti and their possible role in denv infection by the modulation of apoptosis.recently, a salivary gland transcriptome study demonstrated that the transcripts of a putative cystatin gene (seqid aael013287; aacystatins) from aedes aegypti were increased in denv2-infected mosquitoes and that silencing of the aacystatin gene resulted in an increase in denv titres. in this work, aacystatin was biochemically characterized; the purified recombinant inhibitor was able to inhibit typical cysteine proteases with a ki in the nm range. pulldown assays using aag2 cell extracts identi ...201931857170
insecticide resistance in culex quinquefasciatus say, 1823 in brazil: a review.culex quinquefasciatus is a successful invasive species broadly distributed in subtropical regions, including brazil. it is an extremely annoying mosquito due to its nocturnal biting behavior, in high-density populations and it is a potential bridge between sylvatic arbovirus from birds to man in urban territories. herein, we present a review concerning the methods of chemical control employed against cx. quinquefasciatus in brazil since the 1950's and insecticide resistance data registered in t ...201931852489
deducing the role of virus genome-derived piwi-associated rnas in the mosquito-arbovirus arms race.the p-element-induced wimpy testis (piwi)-associated rna (pirna) pathway is known for its role in the protection of genome integrity in the germline of drosophila melanogaster by silencing transposable elements. the pirnas that target transposons originate from pirna clusters in transposon-rich regions of the drosophila genome and are processed by three piwi family proteins. in aedes aegypti and aedes albopictus mosquitoes, which are two of the most important vectors of arthropod-borne viruses ( ...201931850054
mechanisms of transport of h+, na+ and k+, across the distal gastric caecum of larval aedes aegypti.measured changes in ion fluxes, transepithelial potential (tep) and basolateral membrane potential (vb) in response to ion transporter inhibitors were used to assess the mechanisms of transport of h+, na+ and k+, across the distal gastric caecum of larval aedes aegypti, a vector of yellow fever. preparations were stimulated with 5-hydroxytryptamine (5-ht, 10-6 m) in order to maintain stable rates of h+, na+, and k+ transport across the distal caecum. transepithelial potential (tep), basolateral ...201931846613
effect of wolbachia walbb on a positive-sense rna negev-like virus: a novel virus persistently infecting aedes albopictus mosquitoes and cells.the aedes aegypti mosquito is the primary vector of several medically important arboviruses. the endosymbiotic bacterium, wolbachia pipientis, has emerged as a means of blocking transmission of arboviruses such as dengue and zika viruses. one wolbachia strain that has shown potential in field trials is walbb, a naturally occurring wolbachia strain of the asian tiger mosquito aedes albopictus. when transinfected into ae. aegypti, walbb exhibits strong virus inhibition. in addition to modulating a ...201931846415
antidengue potential of leaf extracts of pavetta tomentosa and tarenna asiatica (rubiaceae) against dengue virus and its vector aedes aegypti (diptera: culicidae).the aim of the present study was to screen the anti-dengue potential of crude leaf extracts of two plants from pavetta tomentosa and tarenna asiatica. for larvicidal assay, the acetone extract of both plants showed maximum effects, with the least lc50 and lc90 values (p. tomentosa (5.968 and 7.493 μg/ml) and t. asiatica (1.288 and 1.992 μg/ml)) and the same extract of both plants exhibited better pupicidal potency. the adulticidal activity of both plants (0-60 min interval periods) recorded best ...201931844692
author correction: cartography of odor chemicals in the dengue vector mosquito (aedes aegypti l., diptera/culicidae).an amendment to this paper has been published and can be accessed via a link at the top of the paper.201931844083
nano-emulsification enhances the larvicidal potential of the essential oil of siparuna guianensis (laurales: siparunaceae) against aedes (stegomyia) aegypti (diptera: culicidae).siparuna guianensis (laurales: siparunaceae) has a terpene-rich essential oil with great potential for larvicides. the poor water miscibility of their compounds makes nano-emulsions of great interest for novel bioactive systems, including for control of aedes aegypti (diptera: culicidae). this species is adapted to urban environments with important role in the epidemiology of some arboviruses such as dengue, chikungunya fever, zika, and urban yellow fever. the aim of the present study was to eva ...201931840745
could land cover influence aedes aegypti mosquito populations?aedes aegypti is mainly found in urban environments, where human activity guarantees the permanent availability of potential larval habitats. the present study aimed to test for a possible association between ae. aegypti infestation and land cover classes. from 23 february to 22 may 2015, immature mosquitoes (except eggs) were sampled in artificial containers identified in dwellings in córdoba city, argentina. the proportion of each land cover class was determined by spot-5 (satellites pour 1'ob ...201931840284
genome sequences of chikungunya virus isolates from an outbreak in southwest bangkok in 2018.an outbreak of chikungunya virus (chikv) infection occurred in southwest bangkok during the 2018 rainy season. the envelope glycoprotein e1 gene sequence of the infecting strain belonged to an east/central/south african lineage with alanine at residue 226. mutations in the predicted e1 (k211e) and e2 (v264a) proteins of chikv were identified in chikv-infected patients and in an aedes aegypti mosquito. analysis of the complete genome sequences showed marked differences from the strains causing pr ...201931834526
distribution of insecticide resistance and mechanisms involved in the arbovirus vector aedes aegypti in laos and implication for vector control.the yellow fever mosquito aedes aegypti is the major vector of dengue, yellow fever, zika, and chikungunya viruses. worldwide vector control is largely based on insecticide treatments but, unfortunately, vector control programs are facing operational challenges due to mosquitoes becoming resistant to commonly used insecticides. in southeast asia, resistance of ae. aegypti to chemical insecticides has been documented in several countries but no data regarding insecticide resistance has been repor ...201931830027
effects of larval diets and temperature regimes on life history traits, energy reserves and temperature tolerance of male aedes aegypti (diptera: culicidae): optimizing rearing techniques for the sterile insect programmes.producing high quality sterile males is vital in aedes aegypti rear-and-release birth control strategies. larval diets, rearing temperatures, and their interactions determine the accumulation rates of essential nutrients in larvae, but these factors have been understudied in relation to mass-rearing techniques for producing eminent males.201931823817
social capital is associated with lower mosquito vector indices: secondary analysis from a cluster randomised controlled trial of community mobilisation for dengue prevention in mexico.control of the aedes aegypti mosquito is central to reducing the risk of dengue, zika, chikungunya, and yellow fever. randomised controlled trials, including the camino verde trial in mexico and nicaragua, demonstrate the convincing impact of community mobilisation interventions on vector indices. these interventions might work through building social capital but little is known about the relationship between social capital and vector indices.201931823786
zika virus: a possible emerging threat for bangladesh!zika virus, a member of flaviviridae is the etiology of zika or zika fever or zika virus (zikv) disease characterized by mild symptoms similar to very mild form of dengue or chikungunya. the virus transmits through aedes mosquitoes, particularly by aedes aegypti. the most dangerous effect of zikv infection is the ability of the virus to cause microcephaly and congenital malformation to the newborn baby if the mother is infected. the neurological disorders including guillain-barré syndrome might ...201931819889
successive blood meals enhance virus dissemination within mosquitoes and increase transmission potential.the recent zika virus (zikv) and chikungunya virus epidemics highlight the explosive nature of arthropod-borne viruses (arboviruses) transmitted by aedes spp. mosquitoes1,2. vector competence and the extrinsic incubation period (eip) are two key entomological parameters used to assess the public health risk posed by arboviruses3. these are typically measured empirically by offering mosquitoes an infectious blood meal and temporally sampling mosquitoes to determine the infection and transmission ...201931819213
natural infection of aedes aegypti by chikungunya and dengue type 2 virus in a transition area of north-northeast brazil.dengue fever, chikungunya, and zika are diseases caused by viruses transmitted by aedes aegypti and aedes albopictus. in brazil, the number of human infections is high, but few studies are performed in mosquito vectors. this study aimed to investigate the presence of zika, dengue and chikungunya viruses in ae. aegypti and ae. albopictus from the municipalities of alto alegre, caxias, codó, and são mateus do maranhão, located in the state of maranhão, northeast brazil. the mosquitoes were collect ...201931817553
dengue virus reduces agpat1 expression to alter phospholipids and enhance infection in aedes aegypti.more than half of the world population is at risk of dengue virus (denv) infection because of the global distribution of its mosquito vectors. denv is an envelope virus that relies on host lipid membranes for its life-cycle. here, we characterized how denv hijacks the mosquito lipidome to identify targets for novel transmission-blocking interventions. to describe metabolic changes throughout the mosquito denv cycle, we deployed a liquid chromatography-high resolution mass spectrometry (lc-hrms) ...201931815960
genetic diversity of laboratory strains and implications for research: the case of aedes aegypti.the yellow fever mosquito (aedes aegypti), is the primary vector of dengue, zika, and chikungunya fever, among other arboviral diseases. it is also a popular laboratory model in vector biology due to its ease of rearing and manipulation in the lab. established laboratory strains have been used worldwide in thousands of studies for decades. laboratory evolution of reference strains and contamination among strains are potential severe problems that could dramatically change experimental outcomes a ...201931815934
glopid-r report on chikungunya, o'nyong-nyong and mayaro virus, part 5: entomological aspects.the glopid-r (global research collaboration for infectious disease preparedness) chikungunya (chikv), o'nyong-nyong (onnv) and mayaro virus (mayv) working group has been established to investigate natural history, epidemiology and clinical aspects of infection by these viruses. here, we present a report dedicated to entomological aspects of chikv, onnv and mayv. recent global expansion of chikungunya virus has been possible because chikv established a transmission cycle in urban settings using a ...201931812638
cryo-electron microscopy reveals that sperm modification coincides with female fertility in the mosquito aedes aegypti.manipulating mosquito reproduction is a promising approach to reducing mosquito populations and the burden of diseases they carry. a thorough understanding of reproductive processes is necessary to develop such strategies, but little is known about how sperm are processed and prepared for fertilization within female mosquitoes. by employing cryo-electron microscopy for the first time to study sperm of the mosquito aedes aegypti, we reveal that sperm shed their entire outer coat, the glycocalyx, ...201931811199
assessment of aedes aegypti (diptera: culicidae) males flight ability for sit application: effect of device design, duration of test, and male age.the sterile insect technique (sit) is a pest control method where large numbers of sterile males are released to induce sterility in wild populations. since a successful sit application depends on the released sterile males being competitive with wild males, standard quality control tests are a necessary component of any sit program. flight ability (ability to fly out from a device) is a reliable indicator of insect quality. based on previous studies, we developed four new tubular devices constr ...201931808821
malaria and dengue mosquito vectors from lao pdr show a lack of the rdl mutant allele responsible for cyclodiene insecticide resistance.the gamma-aminobutyric acid (gaba) receptor, rdl, plays important roles in neuronal signaling and is the target of highly effective insecticides. a mutation in rdl, commonly a296s, underlies resistance to several insecticides such as cyclodienes. even though the use of cyclodienes has been banned, the occurrence of mutations substituting a296 is notably high in mosquitoes from several countries. here, we report a survey investigating the prevalence of the rdl mutant allele in mosquitoes from lao ...201931807752
the consequences of brugia malayi infection on the flight and energy resources of aedes aegypti mosquitoes.evidence from experimental infection studies has shown that infected mosquitoes exhibit altered host-seeking behaviours, with suppression and activation of behaviours dependent on the parasite's development stage. the mechanisms are poorly characterised; however, infections can impact mosquito energy reserves, thereby influencing key life-history traits and behaviours. in addition, filarial infection is likely detrimental to flight due to damage caused by developing worms. this study aimed to ev ...201931804546
finding the sweet spot: what levels of larval mortality lead to compensation or overcompensation in adult production?extrinsic mortality impinging on negatively density-dependent populations can result in no change in the number of survivors (compensation) or an increase (overcompensation) by releasing the population from density-dependent effects on survivorship. the relationship between the level of extrinsic mortality (i.e., percentage of mortality) and the level and likelihood of overcompensation is theoretically important, but rarely investigated. we tested the hypothesis that overcompensation occurs belo ...201931803516
diterpenes and their derivatives as promising agents against dengue virus and dengue vectors: a literature-based review.dengue, a mosquito-borne viral disease, causes about 100 million cases of infection annually. it is a major public concern, and if left untreated or improperly diagnosed, may cause serious health problems or even death. historically, dengue has not considered priorities for pharmaceutical companies made the available treatment options. therefore, medicinal scientists are revealing new insights and enabling novel interventions and approaches to dengue prevention and control. diterpenes, a class o ...201931802573
[river boats as a means of expansion of aedes aegypti towards border zones of the peruvian amazon].to assess the entomological risk of aedes aegypti in boats traversing border river routes in loreto.201931800930
investigating male aedes aegypti (diptera: culicidae) attraction to different oviposition containers using various configurations of the sound gravid aedes trap.aedes aegypti (linnaeus), the primary vectors of the arboviruses dengue virus and zika virus, continue to expand their global distributions. in efforts to better control such species, several mosquito control programs are investigating the efficacy of rearing and releasing millions of altered male aedes throughout landscapes to reduce populations and disease transmission risk. unfortunately, little is known about ae. aegypti, especially male, dispersal behaviors within urban habitats. we deploye ...201931799614
drug repurposing of bromodomain inhibitors as potential novel therapeutic leads for lymphatic filariasis guided by multispecies transcriptomics.to better understand the transcriptomic interplay of organisms associated with lymphatic filariasis, we conducted multispecies transcriptome sequencing (rna-seq) on the filarial nematode brugia malayi, its wolbachia endosymbiont wbm, and its laboratory vector aedes aegypti across the entire b. malayi life cycle. in wbm, transcription of the noncoding 6s rna suggests that it may be a regulator of bacterial cell growth, as its transcript levels correlate with bacterial replication rates. for a. ae ...201931796568
cdte-gsh as luminescent biomarker for labeling the larvicidal action of wsmol lectin in aedes aegypti larvae.mosquito-borne arboviruses compromise human health worldwide. due to resistance to chemical insecticides, natural compounds have been studied to combat mosquitoes. previous works have demonstrated a larvicidal activity of the water-soluble moringa oleifera lectin (wsmol) against aedes aegypti, suggesting a mechanism of action based on the interaction between lectin and chitin present in the larvae's peritrophic matrix. in this work, it was investigated the wsmol activity against aedes aegypti la ...201931796242
associations between household environmental factors and immature mosquito abundance in quetzaltenango, guatemala.aedes aegypti-borne diseases are becoming major public health problems in tropical and sub-tropical regions. while socioeconomic status has been associated with larval mosquito abundance, the drivers or possible factors mediating this association, such as environmental factors, are yet to be identified. we examined possible associations between proximity to houses and roads and immature mosquito abundance, and assessed whether these factors and mosquito prevention measures mediated any associati ...201931870343
compatibility between leptolegnia chapmanii and diflubenzuron and neem oil for the control of aedes aegypti.leptolegnia chapmanii is pathogenic to mosquito larvae. the compatibility between l. chapmanii and two insect growth regulators (igr), diflubenzuron and neem oil, was evaluated. l. chapmanii was grown on culture media containing different concentrations of each igr. the mycelial growth was significantly reduced with the highest concentrations of igr (f6,98=268, p<0.05). however, the production of zoospores (f6,56=0.93, p>0.05) and the larval mortality of aedes aegypti (f6,56=0.95, p>0.05) were n ...201931866096
a non-conformational qsar study for plant-derived larvicides against zika aedes aegypti l. vector.a set of 263 plant-derived compounds with larvicidal activity against aedes aegypti l. (diptera: culicidae) vector is collected from the literature, and is studied by means of a non-conformational quantitative structure-activity relationships (qsar) approach. the balanced subsets method (bsm) is employed to split the complete dataset into training, validation and test sets. from 26,775 freely available molecular descriptors, the most relevant structural features of compounds affecting the bioact ...201931865579
intra-host growth kinetics of dengue virus in the mosquito aedes aegypti.dengue virus (denv) transmission by mosquitoes is a time-dependent process that begins with the consumption of an infectious blood-meal. denv infection then proceeds stepwise through the mosquito from the midgut to the carcass, and ultimately to the salivary glands, where it is secreted into saliva and then transmitted anew on a subsequent bite. we examined viral kinetics in tissues of the aedes aegypti mosquito over a finely graded time course, and as per previous studies, found that initial vi ...201931790509
crispr/cas9-mediated gene deletion of the ompa gene in symbiotic cedecea neteri impairs biofilm formation and reduces gut colonization of aedes aegypti mosquitoes.symbiotic bacteria are pervasive in mosquitoes and their presence can influence many host phenotypes that affect vectoral capacity. while it is evident that environmental and host genetic factors contribute in shaping the microbiome of mosquitoes, we have a poor understanding regarding how bacterial genetics affects colonization of the mosquito gut. the crispr/cas9 gene editing system is a powerful tool to alter bacterial genomes facilitating investigations into host-microbe interactions but has ...201931790395
reversal of resistance to the larvicide temephos in an aedes aegypti (diptera: culicidae) laboratory strain from cuba.the objective of this investigation was to know whether the organophosphate temephos resistance developed in larvae from a laboratory strain of aedes aegypti (linnaeus, 1762) from cuba could be reversed. the resistant laboratory strain of ae. aegypti, named san-f6, was left without temephos selection pressure for 12 generations. the level of temephos resistance was determined using who bioassays and mechanisms of metabolic resistance were determined based on enzyme activity levels detected by bi ...201931788689
the expanding pattern of aedes aegypti in southern yunnan, china: insights from microsatellite and mitochondrial dna markers.aedes aegypti, the vector of dengue fever, was first reported in yunnan in 2002. now, this species is found in nine counties in border areas of south-west yunnan. related dengue fever outbreaks have been reported since 2013. the population genetics of ae. aegypti in these areas were studied to explain the expansion history of this species.201931775906
the enemy of my enemy: multiple interacting selection pressures lead to unexpected anti-predator responses.despite being a major selective force, predation can induce puzzling variability in anti-predator responses-from lack of predator aversion to lifelong predator-induced fear. this variability is hypothesised to result from variation in the trade-offs associated with avoiding predators. but critical information on fitness outcomes of these trade-offs associated with anti-predator behaviours is lacking. we tested this trade-off hypothesis in aedes aegypti, by examining oviposition site selection de ...201931773313
assessments of influences of a prolific gut fungus (zancudomyces culisetae) on larval and adult mosquito (aedes aegypti) - associated microbiota.adult mosquitoes inherit a bacterial community from larvae via transstadial transmission, an understudied process that may influence host-microbe interactions. microbes contribute to important host life history traits, and analyzing transmitted microbial communities, the interrelationship between larval and adult-associated microbiota, and factors influencing host-microbe relationships are targets for research. during its larval stage, the yellow fever mosquito (aedes aegypti) hosts the trichomy ...201931757825
infected mosquitoes have altered behavior to repellents: a systematic review and meta-analysis.here we conducted a systematic review and meta-analysis to reach a consensus on whether infected and uninfected mosquitoes respond differently to repellents. after screening 2,316 published studies, theses, and conference abstracts, we identified 18 studies that tested whether infection status modulated the effectiveness of repellents. thirteen of these studies had outcomes available for meta-analysis, and overall, seven repellents were tested (typically deet with 62% of outcomes), six mosquito ...201931755530
arbovirus emergence in the temperate city of córdoba, argentina, 2009-2018.the distribution of arbovirus disease transmission is expanding from the tropics and subtropics into temperate regions worldwide. the temperate city of córdoba, argentina has been experiencing the emergence of dengue virus, transmitted by the mosquito aedes aegypti, since 2009, when autochthonous transmission of the virus was first recorded in the city. the aim of this work is to characterize the emergence of dengue and related arboviruses (zika and chikungunya) in córdoba since 2009. herein, we ...201931754110
stable isotope marking of laboratory-reared aedes aegypti (diptera: culicidae).the use of stable isotope enrichment to mark mosquitoes has provided a tool to study the biology of vector species. in this study, we evaluated isotopic marking of aedes aegypti (l.) (diptera: culicidae) in a laboratory setting. we determined the optimal dosage for marking adult ae. aegypti mosquitoes with 13c and 15n. additionally, ae. aegypti mosquitoes were single and dually marked with 13c and 15n for up to 60 d postemergence without changes to adult body size or transgenerational marking. t ...201931751467
chikungunya virus populations experience diversity- dependent attenuation and purifying intra-vector selection in californian aedes aegypti mosquitoes.chikungunya virus (togaviridae, alphavirus; chikv) is a mosquito-borne global health threat that has been transmitted transiently in the southeastern united states. a primary chikv mosquito vector, aedes aegypti, was recently established in the populous state of california, but the vector competence of californian mosquitoes is unknown. explosive chikv epidemics since 2004 have been associated with the acquisition of mosquito-adaptive mutations that enhance transmission by ae. aegypti or ae. alb ...201931751338
a lamp assay for the rapid and robust assessment of wolbachia infection in aedes aegypti under field and laboratory conditions.with wolbachia-based arbovirus control programs being scaled and operationalised around the world, cost effective and reliable detection of wolbachia in field samples and laboratory stocks is essential for quality control. here we validate a modified loop-mediated isothermal amplification (lamp) assay for routine scoring of wolbachia in mosquitoes from laboratory cultures and the field, applicable to any setting. we show that this assay is a rapid and robust method for highly sensitive and speci ...201931747432
vector distribution and transmission risk of the zika virus in south and central america.zika is of great medical relevance due to its rapid geographical spread in 2015 and 2016 in south america and its serious implications, for example, certain birth defects. recent epidemics urgently require a better understanding of geographic patterns of the zika virus transmission risk. this study aims to map the zika virus transmission risk in south and central america. we applied the maximum entropy approach, which is common for species distribution modelling, but is now also widely in use fo ...201931745446
computational and experimental insights into the chemosensory navigation of aedes aegypti mosquito larvae.mosquitoes are prolific disease vectors that affect public health around the world. although many studies have investigated search strategies used by host-seeking adult mosquitoes, little is known about larval search behaviour. larval behaviour affects adult body size and fecundity, and thus the capacity of individual mosquitoes to find hosts and transmit disease. understanding vector survival at all life stages is crucial for improving disease control. in this study, we use experimental and com ...201931744443
adulticidal activity of metarhizium anisopliae s.l. (hypocreales: clavicipitaceae) native strains from argentina against aedes aegypti (diptera: culicidae).the adulticidal activity of six fungal strains of metarhizium anisopliae (metschn.) sorokin s.l. (hypocreales: clavicipitaceae) against aedes aegypti (l.) (diptera: culicidae) was assessed. these strains (cep 085, cep 087, cep 120, cep 350, cep 404, and cep 423) were isolated from soil samples or nondipteran hosts collected from areas in argentina where ae. aegypti is distributed. bioassays were performed with four conidial concentrations plus a control of each fungal strain to determine the let ...201931742608
vector competence of aedes aegypti and culex quinquefasciatus from the metropolitan area of guadalajara, jalisco, mexico for zika virus.zika virus (zikv) is a mosquito-borne pathogen discovered in the late 40's in uganda during a surveillance program for yellow fever. by 2014 the virus reached eastern island in the americas, and two years later, the virus spread to almost all countries and territories of the americas. the mosquito aedes aegypti has been identified as the main vector of the disease, and several researchers have also studied the vector competence of culex quinquefasciatus in virus transmission. the aim of the pres ...201931740702
electrophysiological responses of eighteen species of insects to fire ant alarm pheromone.olfaction plays a dominant role in insect communication. alarm pheromones, which alert other insects of the same species of impending danger, are a major class of releaser pheromones. the major components of alarm pheromones in red imported fire ants, honeybees and aphids have been identified as 2-ethyl-3,6-dimethylpyrazine (2e-3,6-dp), isopentyl acetate (ipa), and e-β-farnesene (eβf), respectively. in this study, electroantennography (eag) responses to edp (a mixture of 2-ethyl-3,6-dimethylpyra ...201931739452
zika virus dissemination from the midgut of aedes aegypti is facilitated by bloodmeal-mediated structural modification of the midgut basal lamina.the arboviral disease cycle requires that key tissues in the arthropod vector become persistently infected with the virus. the midgut is the first organ in the mosquito that needs to be productively infected with an orally acquired virus. following midgut infection, the virus then disseminates to secondary tissues including the salivary glands. once these are productively infected, the mosquito is able to transmit the virus to a vertebrate host. recently, we described the midgut dissemination pa ...201931739432
zika virus seroprevalence in blood donors from the northeastern region of são paulo state, brazil, between 2015 and 2017.although primarily transmitted by aedes aegypti mosquitos, zika virus (zikv) can also be transmitted by blood transfusion, due to the fact that some of the infected donors can establish asymptomatic viremia. zikv seroprevalence in brazilian blood donors is unknown. the main reason for this gap in the knowledge originates from the difficulty in evaluating zikv humoral immunity due to antigenic cross-reactivity between the different brazilian flaviviruses and, in particular, dengue virus (denv). t ...201931738944
pyriproxyfen treated surface exposure exhibits reproductive disruption in dengue vector aedes aegypti.reduced susceptibility of mosquito vectors to currently used insecticides hampers control interventions. recently pyriproxyfen, an insect growth regulator has been demonstrated to effectively reduce the reproductive potential in vector mosquitoes.201931738760
aedes aegypti immune response and its potential impact on dengue virus transmission.dengue virus (denv) transmission to human populations requires infection of vector mosquitoes as an essential component of the transmission process. denv transmission leads to infections that range from asymptomatic to life-threatening pathologies, such as dengue hemorrhagic fever and dengue shock syndrome. aedes aegypti is the principal vector of denv, and its vector competence consists of the intrinsic factors, genes, molecules, and pathways that allow infection, replication, and dissemination ...201931738698
winning the tug-of-war between effector gene design and pathogen evolution in vector population replacement strategies.while efforts to control malaria with available tools have stagnated, and arbovirus outbreaks persist around the globe, the advent of clustered regularly interspaced short palindromic repeat (crispr)-based gene editing has provided exciting new opportunities for genetics-based strategies to control these diseases. in one such strategy, called "population replacement", mosquitoes, and other disease vectors are engineered with effector genes that render them unable to transmit pathogens. these eff ...201931737050
insight into mosquito gnrh-related neuropeptide receptor specificity revealed through analysis of naturally occurring and synthetic analogs of this neuropeptide family.adipokinetic hormone (akh), corazonin (crz), and the akh/crz-related peptide (acp) are neuropeptides considered homologous to the vertebrate gonadotropin-releasing hormone (gnrh). all three aedes aegypti gnrh-related neuropeptide receptors have been characterized and functionally deorphanized. individually they exhibit high specificity for their native ligands, prompting us to investigate the contribution of ligand structures in conferring receptor specificity for two of these receptors. here, w ...201931736879
zika virus threshold determines transmission by european aedes albopictus mosquitoes.since its emergence in yap island in 2007, zika virus (zikv) has affected all continents except europe. despite the hundreds of cases imported to european countries from zikv-infested regions, no local cases have been reported in localities where the zikv-competent mosquito aedes albopictus is well established. here we analysed the vector competence of european aedes (aegypti and albopictus) mosquitoes to different genotypes of zikv. we demonstrate that ae. albopictus from france was less suscep ...201931735122
an impressive capacity for cold tolerance plasticity protects against ionoregulatory collapse in the disease vector aedes aegypti.the mosquito aedes aegypti is largely confined to tropical and subtropical regions, but its range has recently been spreading to colder climates. as insect biogeography is tied to environmental temperature, understanding the limits of a. aegypti thermal tolerance and their capacity for phenotypic plasticity is important in predicting the spread of this species. in this study, we report on the chill coma onset (cco) and recovery time (ccrt), as well as low-temperature survival phenotypes of larva ...201931732503
insecticidal and antifeedant activities of malagasy medicinal plant (cinnamosma sp.) extracts and drimane-type sesquiterpenes against aedes aegypti mosquitoes.the overuse of insecticides with limited modes of action has led to resistance in mosquito vectors. thus, insecticides with novel modes of action are needed. secondary metabolites in madagascan plants of the genus cinnamosma (canellaceae) are commonly used in traditional remedies and known to elicit antifeedant and toxic effects in insect pests. here we test the hypothesis that extracts of cinnamosma sp. enriched in drimane sesquiterpenes are toxic and/or antifeedant to the yellow fever mosquito ...201931731570
sylvatic dengue virus type 4 in aedes aegypti and aedes albopictus mosquitoes in an urban setting in peninsular malaysia.dengue fever is endemic in malaysia, contributing to significant economic and health burden in the country. aedes aegypti and ae. albopictus are the main vectors of the dengue virus (denv), which circulates in sylvatic and human transmission cycles and has been present in malaysia for decades. the study investigated the presence and distribution of denv in urban localities in the klang valley, peninsular malaysia. a total of 364 ae. aegypti and 1,025 ae. albopictus larvae, and 10 ae. aegypti and ...201931730672
expansion of aedes africanus (diptera: culicidae), a sylvatic vector of arboviruses, into an urban environment of abidjan, côte d'ivoire.in 2008, an outbreak of yellow fever occurred in abidjan. the entomological investigations confirm that abidjan is at risk of yellow fever with a suspicion of the national park of banco (npb) forest as a likely area of re-emergence. this study aims to assess the dispersion of sylvatic vectors of arboviruses from the npb forest to the surrounding areas (andokoi and sagbé). the sampling was done in the rainy season using the who layer-traps technique. among the six species of aedes sampled, aedes ...201931729805
a new approach to improve acoustic trapping effectiveness for aedes aegypti (diptera: culicidae).monitoring mosquito populations is essential to designing and implementing control strategies. recent strategies based on releasing biologically modified mosquitoes have increased the need to effectively monitor mosquito abundance. unfortunately, existing surveillance traps are of limited value due to their high cost and low capture rates. here, we report the results of experiments designed to evaluate the effectiveness of an acoustic trap prototype. stimuli synthesized from recordings of ae. ae ...201931729803
modifying the autocidal gravid ovitrap (ago) with a powered suction fan and additional lures to increase the collections of released aedes aegypti and a natural population of ae. albopictus (diptera: culicidae). 201931729800
the effects of temperature and shading on mortality and development rates of aedes aegypti (diptera: culicidae).urbanization has caused an increase in favorable habitats for aedes aegypti (diptera: culicidae), given their ability to reproduce in small and often non-degradable artificial water-containers. while much work has been done on ae. aegypti biology and ecology in urban landscapes, the role of shading on immature stages as an independent factor from temperature, and any possible interactions between these factors, remains unexamined. we assessed how temperature and shading affected egg hatch-rate, ...201931729799
parity and gonotrophic discordance of females of aedes aegypti and aedes albopictus (diptera: culicidae) in the city of são paulo, sp, brazil.the objective of this study was to assess the parity, presence of blood in the stomach, and the gonotrophic discordance of females of aedes aegypti and aedes albopictus captured in two areas of the city of são paulo. the captures were undertaken monthly, by aspiration, in the period from january, 2015 to august, 2017. all the females of the two species had their midguts and ovaries dissected to determine the presence of blood and the parity/stage of maturation. with regard to parity, 27% and 34% ...201931729798
state-wide survey of aedes aegypti and aedes albopictus (diptera: culicidae) in florida.aedes aegypti and aedes albopictus are invasive mosquito species with geographic ranges that have oscillated within florida since their presence was first documented. local transmission of dengue, chikungunya, and zika viruses serves as evidence of the public health importance of these two species. it is important to have detailed knowledge of their distribution to aid in mosquito control efforts and understand the risk of arbovirus transmission to humans. through a partnership involving the uni ...201931729793
spatial-temporal distribution of aedes (stegomyia) aegypti and locations of recycling units in southeastern brazil.dengue is an arbovirus disease that threatens approximately 200 million people annually worldwide. aedes (stegomyia) aegypti (linnaeus, 1762) is anthropophilic mosquito, extremely well adapted to the urban environment and utilizes varied habitats for egg-laying and development. this study analysed the distribution of mosquito larvae and eggs in urban area of santa bárbara d'oeste, são paulo, brazil. the spatial correlation between locations in which people store recyclable materials and the dist ...201931727179
corrigendum: supersensitive odorant receptor underscores pleiotropic roles of indoles in mosquito ecology.[this corrects the article doi: 10.3389/fncel.2018.00533.].201931762737
semi-field assessment of spinosad in combination with altosid briquet and dudim dt tablets against aedes aegypti mosquito larvae reared in pond water.this study aims at evaluating the larvicidal efficacy of slow-release formulations of bacterial insecticide spinosad blended with two insect growth regulators (igrs), altosid xr-briquets and dudim dt tablets against mosquito larvae of aedes aegypti. this insect is the cause of dengue arthropod-borne viral disease. treatment based on vector control using chemicals poses risks to human and environment, while the strategy based on the use of natural products poses lower risks. the results of the pr ...201931762652
establishment of wolbachia strain walbb in malaysian populations of aedes aegypti for dengue control.dengue has enormous health impacts globally. a novel approach to decrease dengue incidence involves the introduction of wolbachia endosymbionts that block dengue virus transmission into populations of the primary vector mosquito, aedes aegypti. the wmel wolbachia strain has previously been trialed in open releases of ae. aegypti; however, the walbb strain has been shown to maintain higher density than wmel at high larval rearing temperatures. releases of ae. aegypti mosquitoes carrying walbb wer ...201931761702
co2 per se activates carbon dioxide receptors.carbon dioxide has been used in traps for more than six decades to monitor mosquito populations and help make informed vector management decisions. co2 is sensed by gustatory receptors (grs) housed in neurons in the maxillary palps. co2-sensitive grs have been identified from the vinegar fly and mosquitoes, but it remains to be resolved whether these receptors respond to co2 or bicarbonate. as opposed to the vinegar fly, mosquitoes have three gr subunits, but it is assumed that subunits gr1 and ...201931760135
identification of zika virus in immature phases of aedes aegypti and aedes albopictus: a surveillance strategy for outbreak anticipation.a progressive increase in the circulation of arboviruses in tropical countries has been observed, accounting for 700,000 yearly deaths in the world. the main objective of this article was to identify the presence of zika (zikv), dengue (denv), and chikungunya (chikv) viruses in immature stages of aedes aegypti and ae. albopictus. household collections of immature phases of the vectors were carried out in the years 2015 and 2016. a total of 2902 dwellings were visited and the rate of infestation ...201931721902
genomic epidemiology supports multiple introductions and cryptic transmission of zika virus in colombia.colombia was the second most affected country during the american zika virus (zikv) epidemic, with over 109,000 reported cases. despite the scale of the outbreak, limited genomic sequence data were available from colombia. we sought to sequence additional samples and use genomic epidemiology to describe zikv dynamics in colombia.201931718580
the influence of new surveillance data on predictive species distribution modeling of aedes aegypti and aedes albopictus in the united states.the recent emergence or reemergence of various vector-borne diseases makes the knowledge of disease vectors' presence and distribution of paramount concern for protecting national human and animal health. while several studies have modeled aedes aegypti or aedes albopictus distributions in the past five years, studies at a large scale can miss the complexities that contribute to a species' distribution. many localities in the united states have lacked or had sporadic surveillance conducted for t ...201931718099
chemical compositions and mosquito larvicidal activities of essential oils from piper species growing wild in central vietnam.mosquitoes are the deadliest animals on earth and are the vectors of several neglected tropical diseases. recently, essential oils have emerged as potential renewable, cost-effective, and environmentally benign alternatives to synthetic pesticides for control of mosquitoes. in this work, thirteen species of piper were collected from different areas of central vietnam. the essential oils were obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. the essential oils we ...201931717867
dna damage response and repair in perspective: aedes aegypti, drosophila melanogaster and homo sapiens.the maintenance of genomic integrity is the responsibility of a complex network, denominated the dna damage response (ddr), which controls the lesion detection and dna repair. the main repair pathways are base excision repair (ber), nucleotide excision repair (ner), mismatch repair (mmr), homologous recombination repair (hr) and non-homologous end joining repair (nhej). they correct double-strand breaks (dsb), single-strand breaks, mismatches and others, or when the damage is quite extensive and ...201931711518
potential of aedes albopictus and aedes aegypti (diptera: culicidae) to transmit yellow fever virus in urban areas in central africa.yellow fever (yf) remains a major public health issue in sub-saharan africa and south america, despite the availability of an effective vaccine. in africa, most yf outbreaks are reported in west africa. however, urban outbreaks occurred in 2016 in both angola and the democratic republic of congo (drc), and imported cases were reported in chinese workers coming back from africa. in central africa, cameroon and the republic of congo host a high proportion of non-vaccinated populations increasing t ...201931711378
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