Publications

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microorganisms associated with maari, a baobab seed fermented product.a microbiological study was carried out on baobab fermented seeds (maari) obtained from 4 different production sites in burkina faso (mansila, toulfé, ouagadougou and gorgadji). a total of 390 representative isolates comprising 251 aerobic mesophilic bacteria (amb) and 139 lactic acid bacteria (lab) were isolated and identified to species level using a combination of pheno- and genotypic methods including conventional morphological analysis, carbohydrate fermentation profiling, rep-pcr ((gtg)(5) ...201020688411
in vitro multicompartmental bladder model for assessing blockage of urinary catheters: effect of hydrogel coating on dynamics of proteus mirabilis growth.to investigate the effect of a hydrogel coating on the dynamics of bacterial growth in laboratory models of the catheterized bladder. infection of the urinary tract by proteus mirabilis can result in catheter blockage by crystalline biofilm, a common complication in patients undergoing long-term bladder catheterization.201020696356
[characteristic features of urinary tract infection in malnourished children].the aim of this study is to prove a relationship between urinary tract infection and malnutrition in children 0-3 years old, hospitalized in the ivi pediatric clinic, hospital st. mary iaşi, between january 2000 and december 2004.201020700977
adhesion, invasion, and agglutination mediated by two trimeric autotransporters in the human uropathogen proteus mirabilis.fimbriae of the human uropathogen proteus mirabilis are the only characterized surface proteins that contribute to its virulence by mediating adhesion and invasion of the uroepithelia. pmi2122 (aipa) and pmi2575 (taap) are annotated in the genome of strain hi4320 as trimeric autotransporters with "adhesin-like" and "agglutinating adhesin-like" properties, respectively. the c-terminal 62 amino acids (aa) in aipa and 76 aa in taap are homologous to the translocator domains of yada from yersinia en ...201020805336
synthesis and spectrum of the neoglycoside achn-490.achn-490 is a neoglycoside, or "next-generation" aminoglycoside (ag), that has been identified as a potentially useful agent to combat drug-resistant bacteria emerging in hospitals and health care facilities around the world. a focused medicinal chemistry campaign produced a collection of over 400 sisomicin analogs from which achn-490 was selected. we tested achn-490 against two panels of gram-negative and gram-positive pathogens, many of which harbored ag resistance mechanisms. unlike legacy ag ...201020805391
mucinous cystadenoma of ovary in a patient with juvenile polyposis due to 10q23 microdeletion: expansion of phenotype.juvenile polyposis syndrome (jps) is a hereditary condition characterized by development of gastrointestinal polyps, and caused by mutations in smad4 or bmpr1a genes. juvenile polyps can also be found in a related group of syndromes with multisystemic involvement including cowden disease, lhermitte-duclos disease, bannayan-riley-ruvalcaba syndrome, and proteus-like syndrome, all grouped as pten hamartoma tumor syndromes (phts). in all these conditions including jps, polyps manifest in older chil ...201020815035
prevalence of microorganisms of hygienic interest in an organized abattoir in mumbai, india.the magnitude of food-borne illnesses in india is unknown because of lack of surveillance networks. monitoring the prevalence of food-borne pathogens and indicators of contamination in primary production at abattoirs is imperative for creating a data bank and for effective control of such pathogens before they enter the food chain.201020818094
[epidemiology of urinary tract infection in children in marrakech].by its incidence, the polymorphism of its clinical presentation, its potential gravity, the difficulty of realization of urine culture in children, the frequency of the subjacent urologic abnormalities, the urinary tract infection (uti) is an important problem in paediatrics. this work relates to a retrospective study carrying 121 episodes of uti in 111 children taken care in paediatrics services of the teaching hospital mohammed vi in marrakech between march 2005 and march 2009. the age of our ...201020826328
template-based modeling of a psychrophilic lipase: conformational changes, novel structural features and its application in predicting the enantioselectivity of lipase catalyzed transesterification of secondary alcohols.in order to fully explore the structure-function relationship of a proteus lipase (lipk107) that was screened from the soil in our previous study, we have modeled the three-dimensional (3-d) structures of the enzyme in its active and inactive conformations on the basis of crystal structures of burkholderia glumae and pseudomonas aeruginosa lipases in the present study. both homology models suggested that lipk107 possessed a catalytic triad (ser79-asp232-h254), an oxyanion hole (leu13 and gln80) ...201020828637
structural basis of na(+)-independent and cooperative substrate/product antiport in cait.transport of solutes across biological membranes is performed by specialized secondary transport proteins in the lipid bilayer, and is essential for life. here we report the structures of the sodium-independent carnitine/butyrobetaine antiporter cait from proteus mirabilis (pmcait) at 2.3-a and from escherichia coli (eccait) at 3.5-a resolution. cait belongs to the family of betaine/carnitine/choline transporters (bcct), which are mostly na(+) or h(+) dependent, whereas eccait is na(+) and h(+) ...201020829798
bacterial contamination of ram semen, antibiotic sensitivities, and effects on sperm quality during storage at 15°c.this study was designed to determine the degree and type of bacterial contamination of ejaculated semen samples in fertile rams and its consequences on sperm quality during storage. in experiment 1, 68 ejaculates from 36 rams were divided into two aliquots, one of which was used for bacterial culture, while the other one was diluted, stored at 15°c and assessed for plasma membrane integrity and motility at 0, 24 and 48h after dilution. from the 68 ejaculates, 66 were positive for aerobic bacteri ...201020832206
decolorization and biodegradation of reactive blue 13 by proteus mirabilis lag.the decolorization and biodegradation of reactive blue 13 (rb13), a sulphonated reactive azo dye, was achieved under static anoxic condition with a bacterial strain identified as proteus mirabilis lag, which was isolated from a municipal dump site soil near lagos, nigeria. this strain decolorized rb13 (100mg/l) within 5h. the formation of aromatic amine prior to mineralization was supported by fourier transform infrared spectrometry (ftir), which revealed the disappearance of certain peaks, part ...201020832936
enterobacteriaceae act in concert with the gut microbiota to induce spontaneous and maternally transmitted colitis.disruption of homeostasis between the host immune system and the intestinal microbiota leads to inflammatory bowel disease (ibd). whether ibd is instigated by individual species or disruptions of entire microbial communities remains controversial. we characterized the fecal microbial communities in the recently described t-bet(-/-) ×rag2(-/-) ulcerative colitis (truc) model driven by t-bet deficiency in the innate immune system. 16s rrna-based analysis of truc and rag2(-/-) mice revealed distinc ...201020833380
screening of the constituents, antimicrobial and antioxidant activity of endemic origanum hypericifolium o. schwartz & p.h. davis.the chemical compositions, total phenol content, antioxidant and antimicrobial activities with oxidant status of the essential oil from an endemic turkish species, origanum hypericifolium, were investigated. steam distillation (sd) was used to isolate the essential oils, and the chemical analyses were performed by gas chromatography-mass spectrometry (gc-ms). the antimicrobial activity was tested by agar disc diffusion method against morganella morganii (clinic isolate), micrococcus flavus (clin ...201020835958
antibacterial activity of some medicinal mangroves against antibiotic resistant pathogenic bacteria.the antibacterial activity of the leaves and bark of mangrove plants, avicennia marina, a. officinalis, bruguiera sexangula, exoecaria agallocha, lumnitzera racemosa, and rhizophora apiculata was evaluated against antibiotic resistant pathogenic bacteria, staphylococcus aureus and proteus sp. soxhlet extracts of petroleum ether, ethyl acetate, ethanol and water were prepared and evaluated the antibacterial activity using agar diffusion method. most of the plant extracts showed promising antibact ...201020838519
preliminary phytochemical screening and antimicrobial studies of lantana indica roxb.the aim of the present study was to investigate the antimicrobial and preliminary phytochemical properties of lantana indica roxb. the aqueous and organic solvent (ethyl acetate and methanol) extracts from the leaves of lantana indica (verbenaceae) were tested against staphylococcus aureus, bacillus subtilis, steptococcus pyrogens, escherichia coli, proteus vulgaris, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella typhi, aspergillus niger and candida albicans by agar well diffusion met ...201020838528
screening of antibacterial activity of mucus extract of snakehead fish, channa striatus (bloch).the objective of this study is to gain a better understanding of the antimicrobial properties of the mucus extract of snakehead fish, channa striatus against selected human and fish pathogenic microbes.201020707287
lycopersicon esculentum seeds: an industrial byproduct as an antimicrobial agent.lycopersicon esculentum (tomato) fruit is a widely studied matrix. however, only few works focus their attention on its seeds, which constitute a major byproduct of the tomato processing industry. in this study the antimicrobial potential of ten different tomato seed extracts from "bull's heart" and "cherry" varieties were analyzed against gram-positive (staphylococcus aureus, staphylococcus epidermidis, micrococcus luteus, enterococcus faecalis and bacillus cereus) and gram-negative (proteus mi ...201020707344
progressive overgrowth of the cerebriform connective tissue nevus in patients with proteus syndrome.proteus syndrome is a rare overgrowth disorder that almost always affects the skin.201020709429
phylogenetic analysis of the genera proteus, morganella and providencia by comparison of rpob sequences of type and clinical strains suggests the classification of proteus myxofaciens in a new genus cosenzaea gen. nov., as cosenzaea myxofaciens comb. nov.phylogenetic analysis of partial rpob gene sequences of type and clinical strains belonging to different 16s rrna gene-fingerprinting ribogroups within 11 species of enterobacteria of the genera proteus, morganella, and providencia allowed to define rpob clades supported by high bootstrap values and confirmed by >/=2.5% nucleotide divergence. no rpob clade included strains belonging to different species and the majority of the species were confirmed as discrete and homogeneous, since they consti ...201020709916
seasonal variation, chemical composition, and analgesic and antimicrobial activities of the essential oil from leaves of tetradenia riparia (hochst.) codd in southern brazil.the seasonal variation of the chemical composition of the essential oil from fresh leaves of tetradenia riparia (hochst.) codd grown in southern brazil was analyzed by gc-ms, and the analgesic and antimicrobial activities of this oil were assayed. the yield of essential oil ranged from 0.17% to 0.26%, with the maximum amount in winter and the minimum in spring. the results obtained from principal components analysis (pca) revealed the existence of high chemical variability in the different seaso ...201020714310
failure of tigecycline to treat severe clostridium difficile infection.clostridium difficile infection is an emerging and often difficult-to-treat iatrogenic complication. recent data suggest that tigecycline, a novel antibiotic with broad-spectrum antibacterial activity, can be used successfully to treat patients with severe clostridium difficile infection. we report a 70-year-old man who developed severe clostridium difficile infection, was admitted to the intensive care unit and eventually succumbed to complications of his illness despite receiving tigecycline f ...201020715744
efficacy and safety of moxifloxacin in uncomplicated pelvic inflammatory disease: the monalisa study.to evaluate the efficacy and safety of moxifloxacin versus levofloxacin plus metronidazole in uncomplicated pelvic inflammatory disease (upid) in asia.201020716255
antibiotic producing potentials of three freshwater actinomycetes isolated from the eastern cape province of south africa.crude extracts of three actinomycetes species belonging to saccharopolyspora (tr 046 and tr 039) and actinosynnema (tr 024) genera were screened for antibacterial activities against a panel of several bacterial strains. the extracts showed antibacterial activities against both gram-negative and gram-positive test bacteria with inhibition zones ranging from 8 to 28 mm (tr 046); 8 to15 mm (tr 039); and 10 to 13 mm (tr 024). the minimum inhibitory concentrations ranged from 0.078 to 10 mg/ml (tr 04 ...201020717525
[analysis of drug resistance and risk factors of enterobacteriaceae in burn units].to study the risk factors of infection of extended-spectrum beta-lactamases (esbl)-producing strains and drug resistance of enterobacteriaceae that infected burn patients.201020723421
identification and characterization of a phospholipase a2 from the venom of the saw-scaled viper: novel bactericidal and membrane damaging activities.phospholipase a(2) (pla(2)), a common toxic component of snake venom, has been implicated in various pharmacological effects. in this study, a basic myotoxic pla(2), named ectx-i was isolated from echis carinatus snake venom by using gel filtration on superdex g-75, and reverse phase hplc on c18 and c8 sepharose columns. pla(2), ectx-i was 13,861.72 molecular weight as estimated by maldi-tof (15 kd by sds-page), and consisted of 121 amino acid residues cross-linked by seven disulfide bonds. the ...201020723574
inferring the demographic history of the adriatic flexopecten complex.phylogenetic analysis of dna sequences can be a useful tool in taxonomical studies, which has important implications in terms of species traceability. the aim of our study is to solve the controversy regarding the taxonomical status of the flexopecten group, which includes two putative sister species (f. glaber and f. proteus) that co-occur in the adriatic sea and are clearly distinguishable on the basis of morphometric characters. our molecular analysis using three mitochondrial genes (coi, 12s ...201020723607
[effect of methods of application of bacterial ligands on activation of lymphoid organs' effectors in mice].evaluation of differentiation dynamics and localization of various populations of lymphocytes as well as their expression of tlrs during different methods of administration (intranasal, oral, and subcutaneous) of bacterial ligands of opportunistic microorganisms (immunovac-vp-4 vaccine) in experiment on mice.201020737684
purple urine bag syndrome. 201020798099
suprathel-acetic acid matrix versus acticoat and aquacel as an antiseptic dressing: an in vitro study.the treatment of burn wounds is still a challenge regarding the management of antiseptic wound conditioning. especially, in the united states, silver-containing dressings, such as acticoat and aquacel are frequently used. because silver-containing dressings have well-known drawbacks such as an antimicrobial lack against pseudomonas aeruginosa, we sought to develop an alternative dressing method. in previous studies, we could demonstrate the excellent antiseptic properties of acetic acid against ...201020798627
[immunovac-vp4 vaccine used in complex allergen-specific immunotherapy of patients with hay fever].assessment of clinical and immunological effects of use of bacterial therapeutic polycomponent vaccine immunovac-vp4 for allergen-specific immunotherapy (asit) of hay fever.201020799399
brain abscesses during proteus vulgaris bacteremia.proteus vulgaris is only rarely the cause of multiple septic metastases. we describe multiple brain abscesses due to p. vulgaris in an immunocompetent patient successfully treated by antibiotic therapy and colonectomy.201020890628
prevalence and resistance patterns of extended-spectrum and ampc β-lactamase in escherichia coli, klebsiella pneumoniae, proteus mirabilis, and salmonella serovar stanley in a korean tertiary hospital.a total of 100 clinical isolates of escherichia coli (n = 35), klebsiella pneumoniae (n = 63), proteus mirabilis (n = 1), and salmonella serovar stanley (n = 1), showing resistance to cefoxitin, or returning positive in extended-spectrum β-lactamase (esbl) by clinical and laboratory standards institute (clsi) esbl confirmatory method, were studied. the isolates were examined by the boronic acid (ba) disk test, polymerase chain reaction, and pulsed-field gel electrophoresis (pfge) to investigate ...201020854475
prevalence of bacteria and parasites in white ibis in egypt.a field survey was conducted to evaluate the prevalence of bacterial infections among free-living white ibis (nipponia nippon) in which 92 bacterial isolates were recovered from 193 different internal organs of 55 apparently healthy ibis. escherichia coli and salmonella spp. were isolated at rates of 43.6% and 14.5%, respectively. the other bacterial pathogens isolated were shigella spp. (34.5%), enterobacter spp. (21.8%) citrobacter spp. (18.1%), klebsiella pneumonia (16.3), staphylococcus aure ...201020857378
light insensitive silver(i) cyanoximates as antimicrobial agents for indwelling medical devices.ten silver(i) cyanoximates of agl composition (l = nc-c(no)-r, where r is electron withdrawing groups: -cn, -c(o)nr(2), -c(o)r' (alkyl), -c(o)oet, 2-heteroaryl fragments such as 2-pyridyl, 2-benzimidazolyl, 2-benzoxazolyl, 2-benzthiazolyl) were synthesized and characterized using spectroscopic methods and x-ray analysis. crystal structures of four complexes were determined and revealed the formation of two-dimensional (2d) coordination polymers of different complexity in which anions exhibit bri ...201020873734
isolation and characterization of bacteria from the gut of bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion.bombyx mori l. (lepidoptera: bombycidae) have been domesticated and widely used for silk production. it feeds on mulberry leaves. mulberry leaves are mainly composed of pectin, xylan, cellulose and starch. some of the digestive enzymes that degrade these carbohydrates might be produced by gut bacteria. eleven isolates were obtained from the digestive tract of b. mori, including the gram positive bacillus circulans and gram negative proteus vulgaris, klebsiella pneumoniae, escherichia coli, citro ...201020874394
resistance phenotype-genotype correlation and molecular epidemiology of citrobacter, enterobacter, proteus, providencia, salmonella and serratia that carry extended-spectrum β-lactamases with or without plasmid-mediated ampc β-lactamase genes in thailand.extended-spectrum β-lactamases (esbls) and plasmid-mediated ampc β-lactamases (pampcs) have been increasingly reported among less commonly encountered genera of enterobacteriaceae. however, little is known regarding the genetic characteristics of resistance genes and epidemiology of these genera. lack of accurate esbl and pampc detection may adversely affect therapeutic outcomes. this study investigated resistance phenotype-genotype correlation and molecular epidemiology among six genera of ente ...201020880563
detection and pfge analysis of esbl-producing isolates of proteus species isolated from patients at tehran hospitals.the production of extended-spectrum β-lactamases (esbls), in particular tem and ctx-m type, by the strains of proteus spp. is a contributing factor to the emergence of bacterial drug resistance. the aim of this study was to screen and determine of esbls genes among strains of proteus spp. at tehran hospitals.201020885345
antimicrobial activities of single aroma compounds.commercially available aroma samples were evaluated for their olfactory quality by professional perfumers and tested for their antimicrobial activity. agar diffusion and agar-dilution were used as test methods and a set of two gram-positive (staphylococcus aureus and enterococcus faecalis) and four gram-negative bacterial strains (escherichia coli, pseudomonas aeruginosa, proteus vulgaris g, klebsiella pneumoniae and salmonella abony) and a yeast, candida albicans, were the test microorganisms. ...201020922992
proteobactin and a yersiniabactin-related siderophore mediate iron acquisition in proteus mirabilis.proteus mirabilis causes complicated urinary tract infections (utis). while the urinary tract is an iron-limiting environment, iron acquisition remains poorly characterized for this uropathogen. microarray analysis of p. mirabilis hi4320 cultured under iron limitation identified 45 significantly upregulated genes (p ≤ 0.05) that represent 21 putative iron-regulated systems. two gene clusters, pmi0229-0239 and pmi2596-2605, encode putative siderophore systems. pmi0229-0239 encodes a non-ribosomal ...201020923418
novel chalcones and 1,3,5-triphenyl-2-pyrazoline derivatives as antibacterial agents.novel sixteen chalcones and thirteen 1,3,5-triphenyl-2-pyrazolines were synthesized and characterized using ft-ir, hr-mass, nmr (¹h-nmr, ¹³c-nmr, 135 dept, ¹h-¹h cosy and ¹h and ¹³c cosy) and xrd. these compounds were evaluated for their antibacterial activity against six micro-organisms, namely bacillus subtilis ncim 2718, staphylococcus aureus ncim 5021, salmonella typhi ncim 2501, enterobacter aerogenes ncim 5139, pseudomonas aeruginosa ncim 5029, and proteus vulgaris ncim 2813 by twofold dil ...201020925692
extended-spectrum β-lactamases in gram negative bacteria.extended-spectrum β-lactamases (esbls) are a group of plasmid-mediated, diverse, complex and rapidly evolving enzymes that are posing a major therapeutic challenge today in the treatment of hospitalized and community-based patients. infections due to esbl producers range from uncomplicated urinary tract infections to life-threatening sepsis. derived from the older tem is derived from temoniera, a patient from whom the strain was first isolated in greece. β-lactamases, these enzymes share the abi ...201020927289
tigecycline activity: low resistance rates but problematic disc breakpoints revealed by a multicentre sentinel survey in the uk.a multicentre surveillance of tigecycline activity against relevant pathogens in the uk.201020930043
chitosan-based polyherbal toothpaste: as novel oral hygiene product.the objective of the present work was to develop chitosan-based polyherbal toothpaste and evaluate its plaque-reducing potential and efficacy in reduction of dental pathogens.201020930349
essential oil constituents of illicium griffithii and its antimicrobial activity.the essential oil of the fruit of illicium griffithii hook f. et thoms. was extracted using clevenger's apparatus. forty-one compounds were characterized by gas chromatography-mass spectroscopy (gc-ms). 4-methyl-6-(2-propenyl)-1,3-benzodioxole was characterized as the major constituent, followed by linalool amongst the volatile constituents. the essential oil was found to be effective against aspergillus niger, penicillium spp. and saccharomyces cerevisiae and possessed considerable activity aga ...201020931081
a mutational analysis and molecular dynamics simulation of quinolone resistance proteins qnra1 and qnrc from proteus mirabilis.the first report on the transferable, plasmid-mediated quinolone-resistance determinant qnra1 was in 1998. since then, qnr alleles have been discovered worldwide in clinical strains of gram-negative bacilli. qnr proteins confer quinolone resistance, and belong to the pentapeptide repeat protein (prp) family. several prp crystal structures have been solved, but little is known about the functional significance of their structural arrangement.201020932305
understanding interactive characteristics of bioelectricity generation and reductive decolorization using proteus hauseri.this first-attempt study quantitatively explored interactive characteristics of bioelectricity generation and dye decolorization in air-cathode single-chamber microbial fuel cells (mfcs) using indigenous proteus hauseri zmd44. after approx. 15 cycles (30 days) acclimatization in dye-bearing cultures, p. hauseri could express its stable capability of simultaneous bioelectricity generation and color removal (sbp&cr) in mfcs. evidently, appropriate acclimation strategy for formation of the electroc ...201020932743
swab and aspiration specimen collection methods and antibiogram in chronic suppurative otitis media at jos university teaching hospital: which is superior?chronic suppurative otitis media is a very common otologic problem in our environment. appropriate methods for obtaining sample specimens for specific bacteria isolation has generated a lot of controversy. the simplest method available in our environment is the traditional swab method which, however, has been condemned on the basis of introducing contaminants. the objectives of this study were to compare the bacterial yield and the antibiogram of two specimen collection methods: the traditional ...201020935423
domoic acid--a new toxin in the croatian adriatic shellfish toxin profile.this is the first study that presents concentrations of domoic acid detected in the whole shellfish tissue from breeding and harvesting areas along the croatian coast of the adriatic sea during the period 2006 to 2008. shellfish sample analyses after sax cleaning procedures, using a uv-dad-hplc system, showed the presence of domoic acid in four species. the most prevalent of those species were the blue mussel (mytilus galloprovincialis), followed by european flat oyster (ostrea edulis), mediterr ...201020938398
a history of von recklinghausen's nf1.while the study of genetic diseases is a rather recent development in science, von recklinghausen's neurofibromatosis (nf1) has a rich pictorial history, seemingly dating back to the thirteenth century. in 1768, akenside published a scientifically-based description of nf1, recognizing that the monsters of scholars, such as parè and aldrovandi, in fact suffered from a disorder of the nerves. the neuromas of nf1 were first detailed by smith in 1849, but frederick von recklinghausen is credited wit ...201020938857
synergy of nitric oxide and silver sulfadiazine against gram-negative, gram-positive, and antibiotic-resistant pathogens.the synergistic activity between nitric oxide (no) released from diazeniumdiolate-modified proline (proli/no) and silver(i) sulfadiazine (agsd) was evaluated against escherichia coli, enterococcus faecalis, proteus mirabilis, pseudomonas aeruginosa, staphylococcus aureus and staphylococcus epidermidis using a modified broth microdilution technique and a checkerboard-type assay. the combination of no and agsd was defined as synergistic when the fractional bactericidal concentration (fbc) was calc ...201020939612
[the bacteriological profile of bacteriuria in residents of a long-term geriatric facility].little is known on the bacteriological profile of consecutive urine samples in elderly patients institutionalized in nursing homes.201020941920
microbial keratitis in the elderly: a 32-year review.to describe the demographic characteristics, associated factors and causative agents of infectious keratitis in the elderly in a tertiary referral center in são paulo, brazil.201020944931
[time course of microbial colonization of different voice prostheses].for the vocal rehabilitation of laryngectomized patients, voice prostheses are actually one of the best known methods. caused by the surface colonisation with bacteria and fungi the life time of the prostheses is limited to 3-4 months.201020949410
antimicrobial activity of doripenem and other carbapenems against gram-negative pathogens from korea.a total of 950 gram-negative bacterial isolates from patients with bacteremia and urinary tract infections were collected from tertiary-care hospitals in korea. in vitro antimicrobial susceptibility testing was performed using broth microdilution test according to clinical and laboratory standards institute protocol. in general, carbapenems such as doripenem, imipenem, and meropenem were very active against enterobacteriaceae, moraxella catarrhalis, pseudomonas aeruginosa, and acinetobacter sp. ...201020950191
antimicrobial susceptibility of intra-abdominal gram-negative bacilli from europe: smart europe 2008.as part of the worldwide study for monitoring antimicrobial resistance trends (smart), a total of 3,030 clinical isolates of gram-negative bacilli from intra-abdominal infections were collected from 43 hospital centres from 13 european countries during 2008. of 51 species, the most commonly isolated species were escherichia coli (49.3%), followed by klebsiella pneumoniae (10.5%) and pseudomonas aeruginosa (8.6%). respectively, 17.9%, 11.6%, 5.5% and 4.5% of k. pneumoniae, e. coli, proteus mirabi ...201020953653
three enzymatic steps required for the galactosamine incorporation into core lipopolysaccharide.the core lipopolysaccharides (lps) of proteus mirabilis as well as those of klebsiella pneumoniae and serratia marcescens are characterized by the presence of a hexosamine-galacturonic acid disaccharide (αhexn-(1,4)-αgala) attached by an α1,3 linkage to l-glycero-d-manno-heptopyranose ii (l-glycero-α-d-manno-heptosepyranose ii). in k. pneumoniae, s. marcescens, and some p. mirabilis strains, hexn is d-glucosamine, whereas in other p. mirabilis strains, it corresponds to d-galactosamine. previous ...201020959463
survival of phosphate-solubilizing bacteria against dna damaging agents.phosphate-solubilizing bacteria (psbs) were isolated from different plant rhizosphere soils of various agroecological regions of india. these isolates showed synthesis of pyrroloquinoline quinone (pqq), production of gluconic acid, and release of phosphorus from insoluble tricalcium phosphate. the bacterial isolates synthesizing pqq also showed higher tolerance to ultraviolet c radiation and mitomycin c as compared to escherichia coli but were less tolerant than deinococcus radiodurans. unlike e ...201020962905
structure and gene cluster of the o-antigen of escherichia coli o109; chemical and genetic evidences of the presence of l-rhan3n derivatives in the o-antigens of e. coli o109 and o119.o-antigen representing the o-polysaccharide chain of the lipopolysaccharide is the most variable constituent on the cell surface of gram-negative bacteria and a player in their pathogenicity. the o-polysaccharide of escherichia coli o109 was studied by sugar analysis and nuclear magnetic resonance spectroscopy and found to contain a rarely occurring monosaccharide, 2,3-diacetamido-2,3,6-trideoxy-l-mannose (l-rhanac3nac). the following structure of the tetrasaccharide repeating unit of the o-poly ...201020964722
endovascular treatment of disseminated complex aortic vascular malformations in a patient with proteus syndrome. 201020971228
[the incidence of extended spectrum beta-lactamases in proteus mirabilis strains isolated in 2007-2009].proteus sp. rods are opportunistic human pathogens, isolated mainly from urinary tract infections. they are naturally susceptible to most antimicrobials. however, acquisition of genes for extended spectrum beta-lactamases on plasmids and irrational antimicrobial treatment increase amount of multidrug resistant strains and lead to their selection. the aim of this study was to evaluate production of esbls by double disc synergy test and antimicrobial susceptibility of p mirabilis strains by disc d ...201020976952
translational independence between overlapping genes for a restriction endonuclease and its transcriptional regulator.most type ii restriction-modification (rm) systems have two independent enzymes that act on the same dna sequence: a modification methyltransferase that protects target sites, and a restriction endonuclease that cleaves unmethylated target sites. when rm genes enter a new cell, methylation must occur before restriction activity appears, or the host's chromosome is digested. transcriptional mechanisms that delay endonuclease expression have been identified in some rm systems. a substantial subset ...201021092102
[a modified rat model for infection-induced stone formation].to employ a newly modified rat model for infection-induced bladder stone formation.201021092517
c-terminal fragment of amebin promotes actin filament bundling, inhibits acto-myosin atpase activity and is essential for amoeba migration.amebin [formerly termed as apabp-fi; sobczak et al. (2007) biochem. cell biol. 85] is encoded in amoeba proteus by two transcripts, 2672-nt and 1125-nt. a product of the shorter transcript (termed as c-amebin), comprising c-terminal 375 amino-acid-residue fragment of amebin, has been expressed and purified as the recombinant gst-fusion protein. gst-c-amebin bound both to monomeric and filamentous actin. the binding was ca(2+)-independent and promoted filament bundling, as revealed with the trans ...201021094125
[epidemiological and bacteriological characteristics of uropathogen bacteria isolated in a pediatric environment].urinary tract infection (uti) in children is a grave pathology, which requires a fast and effective care. bacteriological and epidemiological data play a determining role in patient's care. we report a retrospective study, which spreads out from january 1st till august 31st, 2009, having concerned hospitalized children for urinary infection in pediatrics service of farhat hached teaching hospital in sousse. our series contained 51 children with a sex ratio of 0.76, an average age of 32 months. t ...201021111582
[occurrence of 16s rrna methylase arma producing enterobacteriaceae in a general hospital in warsaw, poland].resistance to gentamicin, amikacin and kanamycin was screened in 270 clinical isolates of enterobacteriaceae originated from april 19 to may 19, 2010 in a regular hospital in warsaw, poland. most of the isolated bacteria were considered pathogenic. nineteen isolates (7%) were simultaneously resistant to two or three of the tested aminoglycosides. mics of the three aminoglycosides ranged form 128 to 1024 mcg/ml for six isolates. these isolates were suspected to produce 16s rrna methylase. genes e ...201021114012
predation of human pathogens by the predatory bacteria micavibrio aeruginosavorus and bdellovibrio bacteriovorus.the focus of this study was to evaluate the potential use of the predatory bacteria bdellovibrio bacteriovorus and micavibrio aeruginosavorus to control the pathogens associated with human infection.201021114596
ceftaroline activity against pathogens associated with complicated skin and skin structure infections: results from an international surveillance study.to assess the spectrum and potency of ceftaroline, a novel anti-methicillin-resistant staphylococcal cephalosporin, against a 2008 surveillance collection of clinical isolates from patients in the usa and europe.201021115451
clinical aspects and genetic analysis of taiwanese patients with the phenotype of hyper-immunoglobulin e recurrent infection syndromes (hies).hyper-immunoglobulin e recurrent infection syndromes (hies) has characteristic features and identified mutations. this study investigated clinical features and causal candidate mutations in taiwanese patients with the hies phenotype on referral base over 23 million inhabitants.201021120687
laboratory detection of extended-spectrum-beta-lactamase-producing enterobacteriaceae: evaluation of two screening agar plates and two confirmation techniques.the worldwide prevalence of extended-spectrum-beta-lactamase-producing esbl-producing enterobacteriaceae (esbl-e) is increasing, making the need for optimized detection techniques more urgent. in this study we investigated the performance of two esbl-e screening and two esbl-e confirmation techniques. in accordance with the dutch national guidelines (www.wip.nl), a collection of 642 highly resistant enterobacteriaceae strains, as identified by vitek2, was used to test the performances of two scr ...201021123527
necrotising otitis externa: an unusual cause of cranial nerve palsy in a diabetic haemodialysis patient.we present an unusual case of necrotising otitis externa (noe) causing a lower motor neurone facial nerve palsy in a patient with diabetes mellitus and receiving maintenance haemodialysis for end-stage renal disease (esrd). pseudomonas aeruginosa is the most common pathogen isolated in noe, although our case involved the non-typical pathogens aspergillus flavus and proteus mirabilis. we discuss the need for diagnostic rigour and the importance of considering atypical infective pathology in patie ...201021125035
a child with mental retardation and asymmetrical hypertrophy of limbs.a 5-year-old male child presented with progressively increasing asymmetrical overgrowth of limbs, hyperpigmentation on the right half of the body, and mental retardation since 9 months of age.201021132570
photodynamic therapy with a cationic functionalized fullerene rescues mice from fatal wound infections.fullerenes are under intensive study for potential biomedical applications. we have previously reported that a c60 fullerene functionalized with three dimethylpyrrolidinium groups (bf6) is a highly active broad-spectrum antimicrobial photosensitizer in vitro when combined with white-light illumination. we asked whether this high degree of in vitro activity would translate into an in vivo therapeutic effect in two potentially lethal mouse models of infected wounds.201021143031
structure of the o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o60.an o-polysaccharide (o-antigen) was isolated by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o60 and studied by sugar and methylation analyses as well as (1)h and (13)c nmr spectroscopy, including 2d roesy and (1)h,(13)c hmbc experiments in d(2)o and a roesy experiment in a 9:1 h(2)o-d(2)o mixture to reveal correlations for nh protons. it was found that the polysaccharide is built up of linear pentasaccharide repeating units containing an amide of d-glucuronic aci ...201021146157
[comparison of four phenotypic methods to detect extended-spectrum betalactamases].to compare the efficacy of four phenotypic methods for the identification of strains producing extended-spectrum β-lactamases isolated from urine cultures.201021152726
territoriality in proteus: advertisement and aggression. 201021162556
unexpected coregulator range for the global regulator lrp of escherichia coli and proteus mirabilis.the lrp/asnc family of transcription factors links gene regulation to metabolism in bacteria and archaea. members of this family, collectively, respond to a wide range of amino acids as coregulators. in escherichia coli, lrp regulates over 200 genes directly and is well known to respond to leucine and, to a somewhat lesser extent, alanine. we focused on lrp from proteus mirabilis and e. coli, orthologs with 98% identity overall and identical helix-turn-helix motifs, for which a previous study ne ...201021169483
the nationwide study of bacterial pathogens associated with urinary tract infections conducted by the japanese society of chemotherapy.this study was conducted by the japanese society of chemotherapy and is the first nationwide study on bacterial pathogens isolated from patients with urinary tract infections at 28 hospitals throughout japan between january 2008 and june 2008. a total of 688 bacterial strains were isolated from adult patients with urinary tract infections. the strains investigated in this study are as follows: enterococcus faecalis (n = 140), escherichia coli (n = 255), klebsiella pneumoniae (n = 93), proteus mi ...201021174142
synthesis and antimicrobial activity of highly functionalised novel β-lactam grafted spiropyrrolidines and pyrrolizidines.a facile and one-pot synthesis of a series of novel spiropyrrolidines/pyrrolizidines with β-lactam substituent has been accomplished through 1,3-dipolar cycloaddition reaction of alkenyl esters derived from β-lactam aldehyde as dipolarophile with the dipole azomethine ylide derived from 1,2- and 1,3-diketones and secondary amino acids. the synthesized compounds were evaluated for antimicrobial activities and found to exhibit relatively good antibacterial activity at lower concentration against f ...201021177001
asymptomatic bacteriuria among hiv positive pregnant women.the prognostic significance of asymptomatic bacteriuria resides in the observation that the prevalence is, relatively, high in persons with certain medical conditions, such as diabetes mellitus and pregnancy. this prevalence might, even, be higher in patients with human immunodeficiency virus infection. hence, this study set out to determine the prevalence of asymptomatic bacteriuria among symptom free and newly enrolled hiv infected pregnant women attending pmtct unit of antiretroviral clinic o ...201021178431
effect of neem extract against the bacteria isolated from marine fish.marine ornamental fishes are exceedingly valuable due to their high demand in domestic and international markets. there is a growing global interest to rear the fishes in captivity. but problem due to bacteria and fungi are the major hitch in captive condition. since, the use of antibiotics is banned, an attempt was made to ascertain in vitro assay of the neem leaves extract against the bacterial pathogens isolated from infected fishes. bacterial strains isolated from infected regions of the clo ...201021186711
the salmonella genomic island 1 is specifically mobilized in trans by the inca/c multidrug resistance plasmid family.the salmonella genomic island 1 (sgi1) is a salmonella enterica-derived integrative mobilizable element (ime) containing various complex multiple resistance integrons identified in several s. enterica serovars and in proteus mirabilis. previous studies have shown that sgi1 transfers horizontally by in trans mobilization in the presence of the inca/c conjugative helper plasmid pr55.201021187963
thyroid pathology in pten-hamartoma tumor syndrome: characteristic findings of a distinct entity.phosphatase and tensin homolog deleted on chromosome ten (pten)-hamartoma tumor syndrome (phts) is a complex disorder caused by germline inactivating mutations of the pten tumor suppressor gene. phts includes cowden syndrome (cs), bannayan-riley-ruvalcaba syndrome (brrs), and proteus-like syndromes. affected individuals develop both benign and malignant tumors in a variety of tissues, including the thyroid. this study is to better characterize and describe the thyroid pathology within the differ ...201021190448
antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model.the chemical composition and antibacterial activity of essential oils from 10 commonly consumed herbs: citrus aurantium, c. limon, lavandula angustifolia, matricaria chamomilla, mentha piperita, m. spicata, ocimum basilicum, origanum vulgare, thymus vulgaris and salvia officinalis have been determined. the antibacterial activity of these oils and their main components; i.e. camphor, carvacrol, 1,8-cineole, linalool, linalyl acetate, limonene, menthol, a-pinene, b-pinene, and thymol were assayed ...201021030907
an illustrative case of neurofibromatosis type 1 and nf1 microdeletion.we report on a patient with nf1 microdeletion and clinical manifestations that fulfill the diagnostic criteria for neurofibromatosis type 1 but also presenting features reminiscent of proteus syndrome.201021031083
incidence of postoperative eye infections in a private eye hospital in lagos, nigeria.there is a dearth of information on postoperative eye infections in nigeria and most hospitals do not have an infection control program in place.201021033323
occurrence of gram-negative bacteria in hens' eggs depending on their source and storage conditions.the aim of this study was to analyse the qualitative composition of gram-negative microbes, mainly of the family enterobacteriaceae, including pathogenic bacteria such as salmonella, in the albumens and yolks and on the shells of hens' eggs, depending on their source and on the temperature and duration of their storage. a total of 375 table eggs were studied, from a large-scale poultry farm, a small-scale poultry farm and a supermarket. each group was divided into 5 subgroups according to the te ...201021033566
emergence of klebsiella pneumoniae carbapenemase-producing proteus mirabilis in hangzhou, china.carbapenems are used to treat severe infections caused by multi-drug-resistant organisms, however, the emergence of carbapenem-resistant bacterial isolates is becoming an increasing therapeutic challenge. since the first klebsiella (k.) pneumoniae carbapenemase (kpc)-producing k. pneumoniae was reported in 2001, kpc-producing isolates have been found increasingly, specially in enterobacteriaceae. the aim of this study was to characterize the mechanisms of a carbapenem-resistant proteus (p.) mira ...201021034629
16s rdna sequence analysis of culturable marine biofilm forming bacteria from a ship's hull.marine bacteria from the hull of a ship in the form of biofilms or microfouling were isolated, cultured, and identified by phylogenetic analysis using 16s rdna sequences. with an average length of 946 bp, all the 16 sequences were classified using the ribosomal database project (rdp) and were submitted to the national center for biotechnology information. phylogenetic analysis using 16s rdna sequences indicated that the 16 strains belonged to the firmicutes (ik-mb6 exiguobacterium aurantiacum, i ...201021038149
renal stone culture: is it relevant? 201021045482
characterization of epitope specificity of proteus penneri 7 lipopolysaccharide core region.to extend the knowledge on the fragments of proteus penneri lipopolysaccharide core regions, which determine the cross-reactions with specific antibodies, serological studies were performed by use of p. penneri 7 core-specific antiserum and proteus sp. lipopolysaccharides. different reactivity of the tested antiserum with three groups of antigens suggested differences in their core regions' epitope specificity. comparing the results of the serological investigations with the previously determine ...201021060898
[antibacterial antibodies in human immunoglobulins and sera: past and present].to measure levels of several types of antibacterial antibodies in preparations of normal human immunoglobulin as well as in samples of donor sera obtained in 1965 and 2009.201021061576
comparison of bacterial diversity in wheat bran and in the gut of larvae and newly emerged adult of musca domestica (diptera: muscidae) by use of ethidium monoazide reveals bacterial colonization.the objective of the current study is to investigate the bacterial colonization within the gut of the house fly, musca domestica l. (diptera: muscidae), at the larval stage and the bacterial community of the gut of the house fly at the newly emerged adult stage. after using ethidium monoazide to inhibit recovery of nucleic acids from dead bacteria, three polymerase chain reaction (pcr)-amplified 16s rdna libraries from wheat bran, larvae, and newly emerged adults was constructed, analyzed, and c ...201021061987
bacteremia in a multilevel geriatric hospital.bloodstream infection (bsi) is a major cause of morbidity and mortality in hospitals. bacteremia in the elderly remains a diagnostic as well as a therapeutic challenge to the clinician.201021333922
akara ogbomoso: microbiological examination and identification of hazards and critical control points.akara ogbomoso was examined toward the establishment of hazard analysis and critical control point (haccp). the akara was produced in residential buildings with the attendant consequence of contamination. there was ample growth of aerobes, coliforms, staphylococci, shigella and yeast/mold from the samples, water and cowpea pastes. microbial contaminations occur through the processing, which can be corrected through education by adopting good hygienic and manufacturing practices. the critical con ...201021339157
plasmid-mediated quinolone resistance in expanded spectrum beta lactamase producing enterobacteriaceae in morocco.although independently acquired, plasmid-mediated quinolone resistance appears to be linked with extended-spectrum or ampc-type beta-lactamases. since no data are available in african countries, the prevalence of qnr genes at the university hospital ibn rochd, casablanca, morocco, was investigated.201021252459
assessment of effectiveness and safety of ibicella lutea extract in the control of experimental proteus mirabilis urinary tract infection.proteus mirabilis is an important cause of complicated urinary tract infections (uti). like many other microorganisms, p. mirabilis has acquired resistance to many antibiotics. due to the serious effects associated with uropathogenic p. mirabilis and the problems related to the use of antibiotics, alternative strategies for its control must be developed. previously, we studied the effect of ibicella lutea extract, a south american indigenous plant, on in vitro uropathogenicity of p. mirabilis. w ...201021252462
microbial evaluation and public health implications of urine as alternative therapy in clinical pediatric cases: health implication of urine therapy.cultural means of pediatric treatment during ill health is a mainstay in africa, and though urine has been known to contain enteric pathogens, urine therapy is still culturally applicable in some health conditions and also advocated as alternative therapy. the study therefore, is to evaluate the microbial contents and safety of urine.201021293739
synthesis and evaluation of a class of new coumarin triazole derivatives as potential antimicrobial agents.a series of new coumarin-based 1,2,4-triazole derivatives were designed, synthesized and evaluated for their antimicrobial activities in vitro against four gram-positive bacteria (staphylococcus aureus, mrsa, bacillus subtilis and micrococcus luteus), four gram-negative bacteria (escherichia coli, proteus vulgaris, salmonella typhi and shigella dysenteriae) as well as three fungi (candida albicans, saccharomyces cerevisiae and aspergillus fumigatus) by two-fold serial dilution technique. the bio ...201021215620
characterization of the enterobacteriaceae community that developed during storage of minced beef under aerobic or modified atmosphere packaging conditions.the whole cell protein and macrorestriction analysis of dna of enterobacteriaceae isolates recovered from minced beef stored at 0, 5, 10 and 15 °c aerobically and under modified atmosphere packaging consisting of 40% co(2)-30% o(2)-30% n(2) in the presence (map+) and absence (map-) of oregano essential oil were studied. sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) profiles obtained from whole cell protein analysis of the enterobacteriaceae isolates revealed seven groups. ...201021216482
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