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the global burden of listeriosis: a systematic review and meta-analysis.listeriosis, caused by listeria monocytogenes, is an important foodborne disease that can be difficult to control and commonly results in severe clinical outcomes. we aimed to provide the first estimates of global numbers of illnesses, deaths, and disability-adjusted life-years (dalys) due to listeriosis, by synthesising information and knowledge through a systematic review.201425241232
lmtdrm database: a comprehensive database on thiol metabolic gene/gene products in listeria monocytogenes egde.there are a number of databases on the listeria species and about their genome. however, these databases do not specifically address a set of network that is important in defence mechanism of the bacteria. listeria monocytogenes egde is a well-established intracellular model organism to study host pathogenicity because of its versatility in the host environment. here, we have focused on thiol disulphide redox metabolic network proteins, specifically in l. monocytogenes egde. the thiol redox meta ...201425228549
sodium chloride-induced filamentation and alternative gene expression of fts, murz, and gnd in listeria monocytogenes 08-5923 on vacuum-packaged ham.the aim of this study was to examine the filament formation and differential gene expression of listeria monocytogenes 08-5923 grown on refrigerated vacuum-packaged ham products with various nacl concentrations. filament formation of l. monocytogenes was observed on ham products with 1.35% and 2.35% nacl, which was monitored using flow cytometry by measuring forward light scatter. quantitative real-time pcr was used to study the differential expression of genes in filamented cells of l. monocyto ...201425227637
potentiometric aptasensing of listeria monocytogenes using protamine as an indicator.exposure to pathogens in recreational or drinking water is a serious public health concern. it is important to rapidly determine and identify trace levels of pathogens in real environmental samples. we report here on a label-free potentiometric aptasensor for rapid, sensitive, and selective detection of listeria monocytogenes (lm), a pathogen widely distributed in the environment. an aptamer binds specifically to internalin a, a surface protein present in lm cells. the target-binding event preve ...201425220163
anti-listerial synergism of leaf essential oil of metasequoia glyptostroboides with nisin in whole, low and skim milks.to examine the individual and synergistic anti-listerial effect of nisin and leaf essential oil of metasequoia glyptostroboides (m. glyptostroboides) against one of the leading foodborne pathogens listeria monocytogenes (l. monocytogenes) atcc 19116 in milk samples.201425149373
antibacterial effect of silver nanoparticles against four foodborne pathogens.there is increased demand for improved disinfection methods due to microorganisms resistant to multiple antimicrobial agents. numerous types of disinfectants are available with different properties; but the proper disinfectant must be carefully selected for any specific application to obtain the desired antimicrobial effect.201425147658
impact of sod on the expression of stress-related genes in listeria monocytogenes 4b g with/without paraquat treatment.listeria monocytogenes is a foodborne pathogen that causes listeriosis. paraquat can generate reactive oxygen species (ros) in cells, which results in oxidative stress. it was first shown that 1 mm of paraquat inhibited the growth rate of a superoxide dismutase (sod)-deletion mutant (∆sod) generated from l. monocytogenes 4b g but not in the wild-type, and induced the expression of other resistance genes (kat, fri, perr, sigb, and reca) as well as sod in the wild type. interestingly, without para ...201425146690
combination of pulsed electric fields, mild heat and essential oils as an alternative to the ultrapasteurization of liquid whole egg.the production of microbiologically safe liquid whole egg (lwe) by industrial ultrapasteurization is restricted by the high thermal sensitivity of lwe components. this research proposes an alternative treatment based on the application of pulsed electric fields (pef) and mild heat, in the presence of natural essential oils (eos) or their individual components (ics). the obtained results indicate that the successive application of pef (25kv/ and 100kj/kg) followed by heat (60°c during 3.5') to lw ...201425146463
riboswitches. sequestration of a two-component response regulator by a riboswitch-regulated noncoding rna.riboswitches are ligand-binding elements contained within the 5' untranslated regions of bacterial transcripts, which generally regulate expression of downstream open reading frames. here, we show that in listeria monocytogenes, a riboswitch that binds vitamin b12 controls expression of a noncoding regulatory rna, rli55. rli55, in turn, controls expression of the eut genes, whose products enable ethanolamine utilization and require b12 as a cofactor. defects in ethanolamine utilization, or in it ...201425146292
choice of reference sequence and assembler for alignment of listeria monocytogenes short-read sequence data greatly influences rates of error in snp analyses.the wide availability of whole-genome sequencing (wgs) and an abundance of open-source software have made detection of single-nucleotide polymorphisms (snps) in bacterial genomes an increasingly accessible and effective tool for comparative analyses. thus, ensuring that real nucleotide differences between genomes (i.e., true snps) are detected at high rates and that the influences of errors (such as false positive snps, ambiguously called sites, and gaps) are mitigated is of utmost importance. t ...201425144537
genipin cross-linked nanocomposite films for the immobilization of antimicrobial agent.cellulose nanocrystal (cnc) reinforced chitosan based antimicrobial films were prepared by immobilizing nisin on the surface of the films. nanocomposite films containing 18.65 μg/cm(2) of nisin reduced the count of l. monocytogenes by 6.73 log cfu/g, compared to the control meat samples (8.54 log cfu/g) during storage at 4 °c in a ready-to-eat (rte) meat system. film formulations containing 9.33 μg/cm(2) of nisin increased the lag phase of l. monocytogenes on meat by more than 21 days, whereas f ...201425140839
the first invertebrate rig-i-like receptor (rlr) homolog gene in the pacific oyster crassostrea gigas.retinoic acid-inducible gene i (rig-i)-like receptor (rlr) is a pivotal receptor that detects numerous rna and dna viruses and mediates the innate induction of interferons and pro-inflammatory cytokines upon viral infection. in the present study, we cloned and characterized the first rig-i gene in a marine mollusk, crassostrea gigas, and designated it as cgrig-i. the full-length cgrig-i cdna is 3436 bp, including 5'- and 3'-untranslated regions (utrs) of 93 bp and 286 bp, respectively, and an op ...201425107697
effect of lauric arginate, nisin z, and a combination against several food-related bacteria.the effects of lauric arginate (lae) and nisin z, alone or in combination, on cell damage were investigated against escherichia coli o157:h7, listeria monocytogenes and brochothrix thermosphacta, by scanning electron microscopy (sem) and transmission electron microscopy (tem) observations, efflux of potassium and phosphate ions, and growth inhibition. a combination of lae with nisin z caused severe and dramatic changes in the cytoplasmic membrane and cell lysis of both gram-positive and gram-neg ...201425106040
cyclic di-gmp-dependent signaling pathways in the pathogenic firmicute listeria monocytogenes.we characterized key components and major targets of the c-di-gmp signaling pathways in the foodborne pathogen listeria monocytogenes, identified a new c-di-gmp-inducible exopolysaccharide responsible for motility inhibition, cell aggregation, and enhanced tolerance to disinfectants and desiccation, and provided first insights into the role of c-di-gmp signaling in listerial virulence. genome-wide genetic and biochemical analyses of c-di-gmp signaling pathways revealed that l. monocytogenes has ...201425101646
management of pregnant women with presumptive exposure to listeria monocytogenes.: listeriosis is predominantly a foodborne illness, with sporadic and outbreak-related cases tied to consumption of food contaminated with listeria (listeria monocytogenes). the incidence of listeriosis associated with pregnancy is approximately 13 times higher than in the general population. maternal infection may present as a nonspecific, flu-like illness with fever, myalgia, backache, and headache, often preceded by diarrhea or other gastrointestinal symptoms. however, fetal and neonatal infe ...201425098360
pediococcus pentosaceus sb83 as a potential probiotic incorporated in a liquid system for vaginal delivery.pediococcus pentosaceus sb83 is a bacteriocinogenic culture having potential use as a vaginal probiotic. the objective of this study was to evaluate the behaviour of p. pentosaceus sb83 incorporated in a liquid system for eventually formulating a gel for vaginal delivery. the vaginal probiotic incorporated into glycerol was able to survive in simulated vaginal fluid at normal vaginal ph (4.2) and at ph of vaginal infections (5.5 and 6.5). the probiotic can be stored at 4 °c for a longer period o ...201425097107
small molecule deubiquitinase inhibitors promote macrophage anti-infective capacity.the global spread of anti-microbial resistance requires urgent attention, and diverse alternative strategies have been suggested to address this public health concern. host-directed immunomodulatory therapies represent one approach that could reduce selection for resistant bacterial strains. recently, the small molecule deubiquitinase inhibitor wp1130 was reported as a potential anti-infective drug against important human food-borne pathogens, notably listeria monocytogenes and noroviruses. util ...201425093325
integrated air stream micromixer for performing bioanalytical assays on a plastic chip.this paper describes the design, functioning and use of an integrated mixer that relies on air flux to agitate microliter entities of fluid in an embedded microfluidic cavity. the system was fabricated from multiple layers of a thermoplastic elastomer and features circuits for both liquid and air supply along with pneumatic valves for process control. internally-dyed polymer particles have been used to visualize flow within the fluid phase during agitation. numerical modelling of the micromixer ...201425091476
impact of rli87 gene deletion on response of listeria monocytogenes to environmental stress.listeria monocytogenes (lm) is a zoonotic pathogen that widely adapts to various environments. recent studies have found that noncoding rnas (ncrnas) play regulatory roles in lm responses to environmental stress. to understand the role of ncrna rli87 in the response regulation, a rli87 deletion strain lm-δrli87 was constructed by homologous recombination and tested for stress responses to high temperature, low temperature, high osmotic pressure, alcohol, acidity, alkaline and oxidative environme ...201425091276
genomic characterization and expression analysis of five novel il-17 genes in the pacific oyster, crassostrea gigas.interleukin-17 (il-17) is a proinflammatory cytokine that plays an important role in clearing extracellular bacteria and contributes to the pathology of many autoimmune and allergic conditions. in the present study, five novel il-17 homologs were identified by searching and analyzing the pacific oyster genome. all six cgil-17 members (including a previously reported homolog) contained four conserved cysteines that were used in the formation of disulfide bonds. phylogenetic analysis showed that a ...201425090939
use of antimicrobial films and edible coatings incorporating chemical and biological preservatives to control growth of listeria monocytogenes on cold smoked salmon.the relatively high incidence of listeria monocytogenes in cold smoked salmon (css) is of concern as it is a refrigerated processed food of extended durability (repfed). the objectives of this study were to compare and optimize the antimicrobial effectiveness of films and coatings incorporating nisin (nis) and sodium lactate (sl), sodium diacetate (sd), potassium sorbate (ps), and/or sodium benzoate (sb) in binary or ternary combinations on css. surface treatments incorporating nis (25000 iu/ml) ...201425089272
development of double loop-mediated isothermal amplification to detect listeria monocytogenes in food.in this study, a double loop-mediated isothermal amplification (dlamp) based on two target genes hlya and iap was developed for the rapid detection of listeria monocytogenes in food. the results revealed that the detection time and temperature of our dlamp assay for l. monocytogenes were 15 min and 63 °c respectively, with a sensitivity of 10 fg dna of l. monocytogenes per tube. while normal lamp (nlamp) of hlya or iap was 100 fg dna of l. monocytogenes per tube for 45 min and 63 °c. furthermore ...201425086581
modelling the effect of lactic acid bacteria from starter- and aroma culture on growth of listeria monocytogenes in cottage cheese.four mathematical models were developed and validated for simultaneous growth of mesophilic lactic acid bacteria from added cultures and listeria monocytogenes, during chilled storage of cottage cheese with fresh- or cultured cream dressing. the mathematical models include the effect of temperature, ph, nacl, lactic- and sorbic acid and the interaction between these environmental factors. growth models were developed by combining new and existing cardinal parameter values. subsequently, the refe ...201425086348
diversity and distribution of listeria monocytogenes in meat processing plants.listeria monocytogenes is a major concern for the meat processing industry because many listeriosis outbreaks have been linked to meat product consumption. the aim of this study was to elucidate l. monocytogenes diversity and distribution across different spanish meat processing plants. l. monocytogenes isolates (n = 106) collected from food contact surfaces of meat processing plants and meat products were serotyped and then characterised by multilocus sequence typing (mlst). the isolates were s ...201425084653
efficacy of plant-derived compounds combined with hydrogen peroxide as antimicrobial wash and coating treatment for reducing listeria monocytogenes on cantaloupes.the efficacy of four plant-derived antimicrobials (pdas), namely carvacrol, thymol, β-resorcylic acid, and caprylic acid, with or without hydrogen peroxide (hp), as antimicrobial wash and chitosan based coating for reducing listeria monocytogenes (lm) on cantaloupes was investigated. cantaloupe rind plugs inoculated with lm (10(7) cfu/cm(2)) were washed for 3, 6, 10 min at 25 °c or 1, 3, 5 min at 55 or 65 °c in water, or water containing 2% pdas with or without 2% hp. additionally, inoculated ca ...201425084644
radiation therapy combined with listeria monocytogenes-based cancer vaccine synergize to enhance tumor control in the b16 melanoma model.conceptually, the immune system may profoundly influence the efficacy of radiation therapy. compelling evidence has recently emerged revealing the capacity of local radiation therapy (rt) to induce antitumor immune responses and sparked interest in combining rt with immunotherapy to promote tumor-specific immunity. a listeria monocytogenes (lm)-based cancer vaccine engineered to express tumor-associated antigen has been shown to effectively retard tumor growth by cell-mediated immune mechanisms. ...201425083327
attenuated listeria monocytogenes reprograms m2-polarized tumor-associated macrophages in ovarian cancer leading to inos-mediated tumor cell lysis.a principal mechanism by which tumors evade immune-mediated elimination is through immunosuppression. previous approaches to tumor immunotherapy have focused on modifying the immunosuppressive environment with immune checkpoint inhibitors, cytokine therapy, and other modalities with the intent to generate t-cell based anti-tumor immunity. we hypothesized that transformation of the suppressive ovarian cancer microenvironment could be achieved by introduction of the attenuated δacta/δinlb strain o ...201425083323
the rab5-gef function of rin1 regulates multiple steps during listeria monocytogenes infection.listeria monocytogenes is a food-borne pathogenic bacterium that invades intestinal epithelial cells through a phagocytic pathway that relies on the activation of host cell rab5 gtpases. listeria monocytogenes must subsequently inhibit rab5, however, in order to escape lysosome-mediated destruction. relatively little is known about upstream rab5 regulators during l. monocytogenes entry and phagosome escape processes in epithelial cells. here we identify rin1, a ras effector and rab5-directed gua ...201425082076
modeling the growth of listeria monocytogenes on the surface of smear- or mold-ripened cheese.surface-ripened cheeses are matured by means of manual or mechanical technologies posing a risk of cross-contamination, if any cheeses are contaminated with listeria monocytogenes. in predictive microbiology, primary models are used to describe microbial responses, such as growth rate over time and secondary models explain how those responses change with environmental factors. in this way, primary models were used to assess the growth rate of l. monocytogenes during ripening of the cheeses and t ...201425072033
systemic listeria monocytogenes infection in a 2-year-old immunocompetent child.central nervous system infection and sepsis are the most frequently observed clinical presentations of listeriosis infection; however, they are rare in immunocompetent children beyond the neonatal period. in the presented case, we described gastrointestinal involvement, subacute meningitis, sinusitis and sepsis in a two-year-old previously healthy child with acute infection caused by listeria monocytogenes. we suggest that the infection was probably enhanced by an inappropriate corticosteroid tr ...201425060681
draft genome sequence of listeria monocytogenes strain li0521 (syn. hpb7171), isolated in 1983 during an outbreak in massachusetts caused by contaminated cheese.listeria monocytogenes, a pathogenic food-borne bacterium, is the causative agent of both sporadic and outbreak cases of human listeriosis. here, we present the genome sequence of l. monocytogenes reference strain li0521, isolated during an outbreak involving contaminated cheese, which has been used as the model during several proteomic studies.201425059873
listeria meningitis and resultant symptomatic hydrocephalus complicating infliximab treatment for ulcerative colitis.listeria monocytogenes, an uncommon pathogen, should be considered by clinicians as a source of sepsis and meningitis in the immunocompromised individuals, including those on anti-tnf alpha agents. immunosuppressant agents including biologic therapies have transformed the management of various rheumatological and dermatological conditions.(1) we report a case of life-threatening l. monocytogenes sepsis and meningitis in a 75-year-old man receiving infliximab for severe ulcerative colitis (uc).201425057381
genetic classification of listeria monocytogenes serotype 4b strains, including epidemic clones, isolated from retail meat in the tokyo metropolitan area.a food-borne pathogen, listeria monocytogenes serotype 4b, has been frequently isolated from patients with listeriosis, and numerous outbreaks of listeriosis are associated with this serotype. in the present study, we performed subtyping of l. monocytogenes serotype 4b strains on the basis of genetic analyses. thirty-four isolates of serotype 4b were classified into 8 genotypes, namely genotypes 12, 15, 16, 17, 18, 23, 24, and 25, on the basis of the sequence for the partial iap gene. genetic an ...201425056070
instantlabs listeria monocytogenes food safety kit. performance tested method 051304.the instantlabs listeria monocytogenes food safety kit was validated against the international organization for standardization (iso) reference method 11290-1 for the detection of listeria monocytogenes and other listeria species. the matrixes (stainless steel, sealed concrete, ice cream, whole milk, cheddar cheese, raw shrimp, hot dogs, deli turkey, and lettuce) were inoculated with approximately 1 cfu/test portion of l. monocytogenes to generate fractional positives (5-15) in 20 inoculated sam ...201425051634
instantlabs listeria species food safety kit. performance tested methods 041304.the instantlabs listeria species food safetykitwas validated against the international organization for standardization (iso) reference method 11290-1 for the detection of listeria monocytogenes and other listeria species. the matrixes (stainless steel, sealed concrete, cheddar cheese, raw shrimp, and hot dogs) were inoculated with approximately 1 cfu/test portion of various listeria species to generate fractional positives (5-15) in 20 inoculated samples. enrichments were also fractionally inoc ...201425051633
deletion of the membrane protein lmo0412 increases the virulence of listeria monocytogenes.listeria monocytogenes is a facultative intracellular pathogen that causes gastroenteritis, meningitis, encephalitis and maternofetal infections. 20-30% of eubacterial orfs are predicted to encode membrane proteins. the bacterial cytoplasmic membrane is a macromolecular structure, which plays a key role for the pathogenesis. despite this, little knowledge exists regarding the function of cytoplasmic membrane proteins of listeria during infection. here, we investigated a predicted membrane protei ...201425051038
assessment of oligogalacturonide from citrus pectin as a potential antibacterial agent against foodborne pathogens.foodborne diseases are an important public health problem in the world. the bacterial resistance against presently used antibiotics is becoming a public health issue; hence, the discovery of new antimicrobial agents from natural sources attracts a lot of attention. antibacterial activities of oligogalacturonide from commercial microbial pectic enzyme (cpe) treated citrus pectin, which exhibits antioxidant and antitumor activities, against 4 foodborne pathogens including salmonella typhimurium, s ...201425048440
serogrouping and pulsed-field gel electrophoresis analysis of listeria monocytogenes isolates from cases of human infection in hungary 2004–2012 molecular typing of listeria monocytogenes in hungary.eighty isolates of listeria monocytogenes cultured from human infections in hungary between 2004 and 2012 were serotyped by the pcr technique of doumithet al. [9] and characterised by pulsed-field gel electrophoresis (pfge). most of the isolates belonged to two serogroups: 53 isolates (66.3%) to serovar group 4b,4d,4e and 21isolates (25.8%) to serogroup 1/2a,3a. although many pulsotypes were identified a particular pulsotype proved highly excelling comprising of 31 isolates after digestion by bo ...201425046881
dnase i and proteinase k impair listeria monocytogenes biofilm formation and induce dispersal of pre-existing biofilms.current sanitation methods in the food industry are not always sufficient for prevention or dispersal of listeria monocytogenes biofilms. here, we determined if prevention of adherence or dispersal of existing biofilms could occur if biofilm matrix components were disrupted enzymatically. addition of dnase during biofilm formation reduced attachment (<50% of control) to polystyrene. treatment of established 72h biofilms with 100μg/ml of dnase for 24h induced incomplete biofilm dispersal, with <2 ...201425043896
use of mild irradiation doses to control pathogenic bacteria on meat trimmings for production of patties aiming at provoking minimal changes in quality attributes.the objectives of the present work were to assess the use of moderate doses of gamma irradiation (2 to 5 kgy) and to reduce the risk of pathogen presence without altering the quality attributes of bovine trimmings and of patties made of irradiated trimmings. microbiological indicators (coliforms, pseudomonas spp and mesophilic aerobic counts), physicochemical indicators (ph, color and tiobarbituric acid) and sensory changes were evaluated during storage. 5 kgy irradiation doses slightly increase ...201425042241
inhibitory effects of the κ-casein macropeptide isolated from milk protein on the biofilm formation and virulence of listeria monocytogenes.we demonstrate the inhibitory effects of κ-casein macropeptide (cmp) on the biofilm formation and virulence of listeria monocytogenes scott a. the inhibition of biofilm formation by cmp was initially investigated by using the protocol applied for the 96-well microtiter plate assay. low concentrations of cmp (0.1, 0.2, 0.3, 0.4, and 0.5 mg/ml) that were tested resulted in a profound inhibitory effect on biofilm formation at a concentration of 0.4 mg/ml. cmp also significantly repressed the transc ...201425036840
serial transfer of single-cell-derived immunocompetence reveals stemness of cd8(+) central memory t cells.maintenance of immunological memory has been proposed to rely on stem-cell-like lymphocytes. however, data supporting this hypothesis are focused on the developmental potential of lymphocyte populations and are thus insufficient to establish the functional hallmarks of stemness. here, we investigated self-renewal capacity and multipotency of individual memory lymphocytes by in vivo fate mapping of cd8(+) t cells and their descendants across three generations of serial single-cell adoptive transf ...201425035956
the effect of oleanolic and ursolic acids on the hemolytic properties and biofilm formation of listeria monocytogenes.oleanolic acid and ursolic acid are pentacyclic triterpenoids isolated from a variety of medicinal plants, which have antibacterial activity. listeria monocytogenes is a gram-positive facultative pathogen, being the causative agent of listeriosis. the present study was carried out to evaluate the in vitro effect of sub-inhibitory concentrations of both triterpene acids on the pathogenicity determinants of l. monocytogenes: their hemolytic activity and biofilm forming ability. oleanolic and ursol ...201425033658
bi-phasic growth of listeria monocytogenes in chemically defined medium at low temperatures.the present work reports a novel observation regarding the growth of l. monocytogenes in modified welshimer's broth (mwb) at low temperatures. specifically, the direct monitoring of the growth of l. monocytogenes scott a using plate count data revealed that the pathogen displays a bi-phasic growth pattern in mwb at 7 °c. this bi-phasic growth pattern is masked (not observed) when optical density (od) measurements are used to monitor growth due to the inability of od readings to detect l. monocyt ...201425016210
analysing and modelling the growth behaviour of listeria monocytogenes on rte cooked meat products after a high pressure treatment at 400 mpa.various predictive models are available for high pressure inactivation of listeria monocytogenes in food, but currently available models do not consider the growth kinetics of surviving cells during the subsequent storage of products. therefore, we characterised the growth of l. monocytogenes in sliced cooked meat products after a pressurization treatment. two inoculum levels (10(7) or 10(4) cfu/g) and two physiological states before pressurization (freeze-stressed or cold-adapted) were evaluate ...201425016207
assessment of salmonella and listeria monocytogenes level in ready-to-cook poultry meat: effect of various high pressure treatments and potassium lactate concentrations.the objective of this study was to develop a probabilistic model in order to determine the contamination level of salmonella and listeria monocytogenes in ready-to-cook poultry meat, after a high pressure (hp) treatment. the model included four steps: i) reception of raw meat materials, mincing and mixing meat, ii) partitioning and packaging into 200-g modified atmosphere packs, iii) high pressure treatment of the meat, and iv) storage in chilled conditions until the end of the shelf-life. the m ...201425016206
purification and mass spectrometry based characterization of a pediocin produced by pediococcus acidilactici 13.bacteriocins are antimicrobial peptides produced by several bacterial species. among the bacteriocins pediocin-like bacteriocins have a significant inhibitory activity on the foodborne pathogens especially on listeria monocytogenes. this study aims to select a simple and usable purification method to purify/concentrate the antimicrobial peptide and characterization of the bacteriocin produced by pediococcus acidilactici 13 by using proteomic approaches which is a recent omic technology. for puri ...201425015266
a btla-mediated bait and switch strategy permits listeria expansion in cd8α(+) dcs to promote long-term t cell responses.listeria monocytogenes infected cd8α(+) dcs in the spleen are essential for cd8(+) t cell generation. cd8α(+) dcs are also necessary for listeria expansion and dissemination within the host. the mechanisms that regulate cd8α(+) dcs to allow listeria expansion are unclear. we find that activating the b and t lymphocyte attenuator (btla), a coinhibitory receptor for t cells, suppresses, while blocking btla enhances, both the primary and memory cd8 t cell responses against listeria. btla(-/-) mice ...201425011109
characterization of listeria monocytogenes strains isolated during 2011-2013 from human infections in switzerland.listeria monocytogenes, an emerging foodborne pathogen, can cause in the population at risk severe infections that are associated with high case fatality rates. a total of 93 l. monocytogenes strains isolated from different patients in switzerland from july 2011 to september 2013 were further characterized. septicemia was reported for 74.2% of the patients, meningitis for 10.8%, and abortion for 3.2%. the majority of the strains belonged to serotype 1/2a (n=58) followed by serotype 4b (n=28), 1/ ...201425007293
reservoirs of listeria species in three environmental ecosystems.soil and water are suggested to represent pivotal niches for the transmission of listeria monocytogenes to plant material, animals, and the food chain. in the present study, 467 soil and 68 water samples were collected in 12 distinct geological and ecological sites in austria from 2007 to 2009. listeria was present in 30% and 26% of the investigated soil and water samples, respectively. generally, the most dominant species in soil and water samples were listeria seeligeri, l. innocua, and l. iva ...201425002422
response to comment on "tim-3 directly enhances cd8 t cell responses to acute listeria monocytogenes infection". 201424994908
comment on "tim-3 directly enhances cd8 t cell responses to acute listeria monocytogenes infection". 201424994907
perinatal immunization with vaccine-grade listeria monocytogenes provides protection against murine th2 airway inflammation.asthma is a chronic respiratory disorder that leads to inflammation and narrowing of the airways. its global prevalence has attained epidemic levels and treatment options that reach beyond temporary relief of symptoms are urgently needed. since the processes leading to clinically symptomatic asthma start early in life, we set out to systematically evaluate a neonatal immunotherapeutic based on listeria monocytogenes (lm) for the control of allergic sensitization.201424991458
schistosoma mansoni soluble egg antigens enhance listeria monocytogenes vector hiv-1 vaccine induction of cytotoxic t cells.vaccines are an important public health measure for prevention and treatment of diseases. in addition to the vaccine immunogen, many vaccines incorporate adjuvants to stimulate the recipient's immune system and enhance vaccine-specific responses. while vaccine development has advanced from attenuated organism to recombinant protein or use of plasmid dna, the development of new adjuvants that safely increase immune responses has not kept pace. previous studies have shown that the complex mixture ...201424990901
inactivation kinetics of escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes in ready-to-eat sliced ham by near-infrared heating at different radiation intensities.the aim of this study was to investigate the inactivation kinetics of salmonella enterica serovar typhimurium, escherichia coli o157:h7, and listeria monocytogenes on ready-to-eat sliced ham by near-infrared (nir) heating as a function of the processing parameter, radiation intensity. precooked ham slices inoculated with the three pathogens were treated at different nir intensities (ca. 100, 150, and 200 μw/cm(2)/nm). an increase in the applied radiation intensity resulted in a gradual increase ...201424988034
metal-chelating active packaging film enhances lysozyme inhibition of listeria monocytogenes.several studies have demonstrated that metal chelators enhance the antimicrobial activity of lysozyme. this study examined the effect of metal-chelating active packaging film on the antimicrobial activity of lysozyme against listeria monocytogenes. polypropylene films were surface modified by photoinitiated graft polymerization of acrylic acid (pp-g-paa) from the food contact surface of the films to impart chelating activity based on electrostatic interactions. pp-g-paa exhibited a carboxylic ac ...201424988022
low occurrence of listeria monocytogenes on bovine hides and carcasses in minas gerais state, brazil: molecular characterization and antimicrobial resistance.listeria monocytogenes occurrence was assessed in three slaughterhouses located in minas gerais state, brazil, by analysis of 209 bovine carcasses. four sponge samples were obtained from each carcass in different steps (a, from hide, before bleeding; b, after hide removal; c, after evisceration; and d, after end washing), resulting in a total of 836 samples. the samples were tested for the presence of l. monocytogenes according to the international organization for standardization 11290-1, and p ...201424988021
the receptor for the complement c3a anaphylatoxin (c3ar) provides host protection against listeria monocytogenes-induced apoptosis.listeria monocytogenes is a gram-positive intracellular bacterium that is acquired through tainted food and may lead to systemic infection and possible death. despite the importance of the innate immune system in fighting l. monocytogenes infection, little is known about the role of complement and its activation products, including the potent c3a anaphylatoxin. in a model of systemic l. monocytogenes infection, we show that mice lacking the receptor for c3a (c3ar(-/-)) are significantly more sen ...201424981453
the essential oil profiles and antibacterial activity of six wild cinnamomum species.the essential oil composition of six species of wild cinnamomum found in borneo was investigated. the oils were obtained from bark by hydrodistillation and the volatile chemical profile was obtained via gas chromatography-mass spectrometry (gcms). a total of 65 volatile constituents were identified, where the essential oils of the studied specimens contained high contents of oxygenated monoterpenes. eucalyptol (1.2-31.1%), terpinen-4-ol (7.9-22.1%), eugenol (0.4-37.9%) and α-cadinol (0.4-1.8%) w ...201425918818
[septic vasculitis caused by listeria monocytogenes].septic vasculitis is a medium and small-vessel vasculitis caused by direct action of pathogens, associated with bacteremia. it is an uncommon condition; clinical manifestations include purpura, ulcers and vesicles-pustules. most cases of septic vasculitis are related to meningococcemia. there are no cases reported in medical literature associated to listeria spp. we report a case of a 71 year-old man who presented sepsis by listeria monocytogenes, and then evolved with purpuric skin lesions. ski ...201425679934
[prosthetic hip infection due to listeria monocytogenes: review of the literature and case report].with the ageing of the population, articular prosthetic replacements are becoming more and more frequent. one of the most feared complications is prosthetic infection, mostly due to bacteria of the cutaneous flora. listeria monocytogenes is rarely the cause. this paper describes the management of a hip prosthetic infection due to listeria monocytogenes. the patient was cured with antimicrobial therapy and a two-stage exchange. this case report creates an opportunity to review the literature in t ...201425549378
whole-genome sequencing identifies an atypical listeria monocytogenes strain isolated from pet foods.four listeria isolates, including an atypical strain, were isolated from various pet foods and sequenced. we report here the draft genome sequences of these isolates and a comparative genomic analysis with a closely related human clinical isolate. an analysis of the atypical strain identified a frameshift mutation in the prfa gene.201425540336
isolation of listeria monocytogenes from milks used for iranian traditional cheese in lighvan cheese factories.traditional lighvan cheese is a semi-hard cheese which has a popular market in iran and neighboring countries. the aim of this study was evaluating the contamination of milks used for lighvan cheese making with listeria monocytogenes. raw milk samples were randomly collected from different cheese producing factories (sampling carried out from large milk tanks used cheese making in factories). isolation of l. monocytogenes was performed according to iso 11290 and biochemical tests were done to id ...201425528910
proteomic differences between listeria monocytogenes isolates from food and clinical environments.listeria monocytogenes is an organism associated with a wide range of foods. it causes listeriosis, a severe illness that mainly affects people with weakened immune systems. proteomic profiles of three different l. monocytogenes isolates were studied using 1d sds page, 2de and mass spectrometry. the protein banding patterns generated by 1d sds page of three strains of l. monocytogenes were found to be similar. visual observations from 2de gel maps revealed that certain spots appeared to have int ...201425513735
microbiological challenge testing for listeria monocytogenes in ready-to-eat food: a practical approach.food business operators (fbos) are the primary responsible for the safety of food they place on the market. the definition and validation of the product's shelf-life is an essential part for ensuring microbiological safety of food and health of consumers. in the frame of the regulation (ec) no 2073/2005 on microbiological criteria for foodstuffs, fbos shall conduct shelf-life studies in order to assure that their food does not exceed the food safety criteria throughout the defined shelf-life. in ...201427800369
regulation of autophagy by e3 ubiquitin ligase rnf216 through becn1 ubiquitination.autophagy is an evolutionarily conserved biological process involved in an array of physiological and pathological events. without proper control, autophagy contributes to various disorders, including cancer and autoimmune and inflammatory diseases. it is therefore of vital importance that autophagy is under careful balance. thus, additional regulators undoubtedly deepen our understanding of the working network, and provide potential therapeutic targets for disorders. in this study, we found tha ...201425484083
complete genome sequence of listeria monocytogenes lm60, a strain with an enhanced cold adaptation capacity.the complete genome sequence of listeria monocytogenes lm60, a fast cold-adapting serotype 1/2a human isolate, is presented.201425477407
metal ion homeostasis in listeria monocytogenes and importance in host-pathogen interactions.listeria monocytogenes is responsible for one of the most life-threatening food-borne infections and the leading cause of food-poisoning associated deaths in the uk. infection may be of the unborn/newly born infant where disease may manifest as listeric abortion, stillbirth or late-onset neonatal listeriosis, while in adults, infection usually affects the central nervous system causing meningitis. crucial to the survival of l. monocytogenes, both inside and outside the host, is its ability to ac ...201425476765
influence of temperature, glucose, and iron on sinigrin degradation by salmonella and listeria monocytogenes.factors, including ph, temperature, glucose concentration, and iron compounds, affect the activity of plant myrosinase and, consequently, endogenous glucosinolate degradation. these factors also may affect glucosinolate degradation by bacterial myrosinase. therefore, this study examined the effect of temperature (4 to 21°c), glucose (0.05 to 1.0%), and iron (10 mm ferrous or ferric) on sinigrin degradation by salmonella or listeria monocytogenes cocktails in mueller-hinton broth and the effect o ...201425474062
controlling attachment and growth of listeria monocytogenes in polyvinyl chloride model floor drains using a peroxide chemical, chitosan-arginine, or heat.listeria monocytogenes can colonize a poultry processing plant as a resident in floor drains. limiting growth and attachment to drain surfaces may help lessen the potential for cross-contamination of product. the objective of this study was to compare a hydrogen peroxide-peroxyacetic acid-based chemical to chitosan-arginine or heat to prevent attachment of or destroy existing l. monocytogenes on the inner surface of model floor drains. l. monocytogenes was introduced to result in about 10(9) pla ...201425474061
analysis of listeria monocytogenes strain distribution in a pork slaughter and cutting plant in the province of quebec.following the 2008 canadian listeriosis outbreak associated with ready-to-eat (rte) meat products, regulations on the presence of listeria monocytogenes in rte food production facilities were modified by health canada, confirming the need to control this pathogen, not only in the final product but also in the plant environment. information on the occurrence of this microorganism during the early steps of production, such as the slaughtering process and in the cutting area, is scarce in canada. i ...201425474060
listeria monocytogenes serotype prevalence and biodiversity in diverse food products.the aim of this study was to assess serotype prevalence and biodiversity of listeria monocytogenes strains isolated from diverse food products, i.e., minced pork, fruits, and vegetables. three hundred twenty-six samples previously purchased from supermarkets and street markets within the athens area were studied for l. monocytogenes prevalence. a total of 121 strains were isolated from the 36 samples that were positive for l. monocytogenes. serotyping was performed with multiplex pcr, and biodiv ...201425474059
inactivation of listeria monocytogenes by disinfectants and bacteriophages in suspension and stainless steel carrier tests.to simulate food contact surfaces with pits or cracks, stainless steel plates with grooves (depths between 0.2 and 5 mm) were constructed. these plates were artificially contaminated with listeria monocytogenes in clean conditions, with organic soiling, or after 14 days of biofilm formation after which inactivation of the pathogen by suma tab d4 (sodium dichloroisocyanurate, 240 and 300 mg/liter), suma bac d10 (quaternary ammonium compound, 740 mg/liter), and bacteriophage suspension (listex p10 ...201425474045
identification of listeria monocytogenes determinants required for biofilm formation.listeria monocytogenes is a gram-positive, food-borne pathogen of humans and animals. l. monocytogenes is considered to be a potential public health risk by the u.s. food and drug administration (fda), as this bacterium can easily contaminate ready-to-eat (rte) foods and cause an invasive, life-threatening disease (listeriosis). bacteria can adhere and grow on multiple surfaces and persist within biofilms in food processing plants, providing resistance to sanitizers and other antimicrobial agent ...201425517120
genetic distance in the whole-genome perspective on listeria monocytogenes strains f2-382 and nihs-28 that show similar subtyping results.genome subtyping approaches could provide useful epidemiological information regarding food pathogens. however, the full genomic diversity of strains that show similar subtyping results has not yet been completely explored. most subtyping methods are based on the differences of only a portion of the genome. we investigated two draft genome sequences of listeria monocytogenes strain f2-382 and nihs-28, which have been identified as closely related strains by subtyping (identical multi-virulence-l ...201425492229
suspension of thymic emigration promotes the maintenance of antigen-specific memory t cells and the recall responses.thymic involution is evolutionarily conserved and occurs early in life. however, the physiological significance remains elusive of this seemingly detrimental process. the present study investigated the potential impact of altered thymic output on t cell memory using ovalbumin (ova) expressed by listeria monocytogenes as a model antigen. suspension of thymic emigration by thymectomy was shown to lead to a marked increase in the frequency and total number of ova-specific memory t cells. in contras ...201425450389
comparative analysis of crispr loci in different listeria monocytogenes lineages.listeria monocytogenes, an important food-borne pathogen, causes high mortality rate of listeriosis. pan-genomic comparisons revealed the species genome of l. monocytogenes is highly stable but not completely clonal. the population structure of this species displays at least four evolutionary lineages (i-iv). isolates of different lineages displayed distinct genetic, phenotypic and ecologic characteristics, which appear to affect their ability to be transmitted through foods and to cause human d ...201425445602
[sausage-related listeria monocytogenes infection].infection with listeria monocytogenes is rare and mainly seen in immunosuppressed patients. infection with l. monocytogenes has a mortality rate of 30%. we present a case report of l. monocytogenes bacteraemia and endocarditis in a 70-year-old man with several co-morbidities and following four major surgical procedures. this illustrates the findings and characteristics in one of the 16 patients who died in 2013 and 2014 this summer due to sausage-related l. monocytogenes infection.201425430580
two outbreaks of listeria monocytogenes infection, northern spain.in the province of gipuzkoa, spain (≈700,000 inhabitants), 7-12 episodes of human listeriosis were recorded annually during 2009-2012. however, during january 2013-february 2014, 27 episodes were detected, including 11 pregnancy-associated cases. fifteen cases in 2 epidemiologically unrelated outbreaks were caused by a rare type of listeria monocytogenes, sequence type 87 serotype 1/2b.201425418800
splenectomy alters distribution and turnover but not numbers or protective capacity of de novo generated memory cd8 t-cells.the spleen is a highly compartmentalized lymphoid organ that allows for efficient antigen presentation and activation of immune responses. additionally, the spleen itself functions to remove senescent red blood cells, filter bacteria, and sequester platelets. splenectomy, commonly performed after blunt force trauma or splenomegaly, has been shown to increase risk of certain bacterial and parasitic infections years after removal of the spleen. although previous studies report defects in memory b- ...201425414706
committee opinion no. 614: management of pregnant women with presumptive exposure to listeria monocytogenes.listeriosis is predominantly a foodborne illness, with sporadic and outbreak-related cases tied to consumption of food contaminated with listeria (listeria monocytogenes). the incidence of listeriosis associated with pregnancy is approximately 13 times higher than in the general population. maternal infection may present as a nonspecific, flu-like illness with fever, myalgia, backache, and headache, often preceded by diarrhea or other gastrointestinal symptoms. however, fetal and neonatal infect ...201425411758
prevalence and populations of listeria monocytogenes in meat products retailed in sao paulo, brazil.this study evaluated the prevalence of the populations and serotypes of listeria monocytogenes in 552 refrigerated samples of ground beef, chicken leg, hot dog, and pork sausage collected in supermarkets in the city of sao paulo, sp, brazil, between may 2008 and july 2009. the supermarkets were selected after stratification by geographical region and by random draw. tests for presence and enumeration of l. monocytogenes were based on iso 11290-1:1996/amd.1:2004 and iso 11290-2:1998 methods, resp ...201425407460
analysis of carbon substrates used by listeria monocytogenes during growth in j774a.1 macrophages suggests a bipartite intracellular metabolism.intracellular bacterial pathogens (ibps) are dependent on various nutrients provided by the host cells. different strategies may therefore be necessary to adapt the intracellular metabolism of ibps to the host cells. the specific carbon sources, the catabolic pathways participating in their degradation, and the biosynthetic performances of ibps are still poorly understood. in this report, we have exploited the technique of (13)c-isotopologue profiling to further study the carbon metabolism of li ...201425405102
multivariate analysis reveals differences in biofilm formation capacity among listeria monocytogenes lineages.biofilm formation capacity evaluated under identical conditions differs among listeria monocytogenes lineages. the approach of using one set of factors or one variable at a time fails to explain why some lineages are more prevalent than others in certain environments. this study proposes the use of multivariate analysis to compare biofilm formation by various strains and describes the ecological niches of l. monocytogenes lineages. nutrient availability, temperature, ph and water activity (aw) a ...201425397897
the antimicrobial activity of liquidambar orientalis mill. against food pathogens and antioxidant capacity of leaf extracts.medicinal plants are an important source of substances which are claimed to induce antimicrobial, antimutagenic and antioxidant effects. many plants have been used due to their antimicrobial treatments. antimicrobial and antioxidant activities of l. orientalis have not been reported to the present day. the aim of this work was to investigate of the antimicrobial and antioxidant potentials of different extracts from l. orientalis.201425395700
quantitative proteome analyses identify prfa-responsive proteins and phosphoproteins in listeria monocytogenes.protein phosphorylation is a major mechanism of signal transduction in bacteria. here, we analyzed the proteome and phosphoproteome of a wild-type strain of the food-borne pathogen listeria monocytogenes that was grown in either chemically defined medium or rich medium containing glucose. we then compared these results with those obtained from an isogenic prfa* mutant that produced a constitutively active form of prfa, the main transcriptional activator of virulence genes. in the prfa* mutant gr ...201425383790
intestinal expression of fas and fas ligand is upregulated by bacterial signaling through tlr4 and tlr5, with activation of fas modulating intestinal tlr-mediated inflammation.tlrs play an important role in mediating intestinal inflammation and homeostasis. fas is best studied in terms of its function in apoptosis, but recent studies demonstrate that fas signaling may mediate additional functions such as inflammation. the role of fas, and the fas ligand (fasl), in the intestine is poorly understood. the aim of this study was to evaluate potential cross-talk between tlrs and fas/fasl system in intestinal epithelial cells (iecs). iecs were stimulated with tlr ligands, a ...201425378591
in situ proteolysis, crystallization and preliminary x-ray diffraction analysis of a vhh that binds listeria internalin b.the variable region of camelid heavy-chain antibodies produces the smallest known antibody fragment with antigen-binding capability (a vhh). the vhh r303 binds internalin b (inlb), a virulence factor expressed by the pathogen listeria monocytogenes. inlb is critical for initiation of listeria infection, as it binds a receptor (c-met) on epithelial cells, triggering the entry of bacteria into host cells. inlb is surface-exposed and is required for virulence, hence a vhh targeting inlb has potenti ...201425372824
geographical and meteorological factors associated with isolation of listeria species in new york state produce production and natural environments.listeria species have been isolated from diverse environments, often at considerable prevalence, and are known to persist in food processing facilities. the presence of listeria spp. has been suggested to be a marker for listeria monocytogenes contamination. therefore, a study was conducted to (i) determine the prevalence and diversity of listeria spp. in produce production and natural environments and (ii) identify geographical and/or meteorological factors that affect the isolation of listeria ...201425364926
inactivation of pathogens during aerobic composting of fresh and aged dairy manure and different carbon amendments.two separate studies were conducted to address the condition and the type of feedstocks used during composting of dairy manure. in each study, physical (temperature), chemical (ammonia, volatile acids, and ph), and biological (salmonella, listeria monocytogenes, and escherichia coli o157:h7) parameters were monitored during composting in bioreactors to assess the degree to which they were affected by the experimental variables and, ultimately, the ability of the chemical and physical parameters ...201425364925
genetic and phenotypic characterization of listeria monocytogenes from human clinical cases that occurred in portugal between 2008 and 2012.listeria monocytogenes infection (listeriosis) is an uncommon but severe foodborne illness that affects mainly individuals with recognized underlying conditions: the elderly, immunocompromised individuals, and pregnant women and their fetuses. the aim of this study was to obtain epidemiological data on cases of listeriosis occurring in portugal from 2008 through 2012, collected in hospitals on a voluntary basis. l. monocytogenes isolates were characterized by genoserotyping by multiplex polymera ...201425361468
the listeria small rna rli27 regulates a cell wall protein inside eukaryotic cells by targeting a long 5'-utr variant.listeria monocytogenes is a bacterial pathogen whose genome encodes many cell wall proteins that bind covalently to peptidoglycan. some members of this protein family have a key role in virulence, and recent studies show that some of these, such as lmo0514, are upregulated in bacteria that colonize eukaryotic cells. the regulatory mechanisms that lead to these changes in cell wall proteins remain poorly characterized. here we studied the regulation responsible for increased lmo0514 protein level ...201425356775
listeria infection in chinese pregnant women and neonates from shandong.this study aims to investigate the characters of pregnant women and neonates with listeria monocytogenes infections (l. monocytogenes) in shandong of china.201425356132
bacterial and cellular rnas at work during listeria infection.listeria monocytogenes is an intracellular pathogen that can enter and invade host cells. in the course of its infection, rna-mediated regulatory mechanisms provide a fast and versatile response for both the bacterium and the host. they regulate a variety of processes, such as environment sensing and virulence in pathogenic bacteria, as well as development, cellular differentiation, metabolism and immune responses in eukaryotic cells. the aim of this article is to summarize first the rna-mediate ...201425340833
foodborne illness, australia, circa 2000 and circa 2010.foodborne disease is a major public health problem worldwide. to examine changes in foodborne illness in australia, we estimated the incidence, hospitalizations, and deaths attributed to contaminated food circa 2010 and recalculated estimates from circa 2000. approximately 25% of gastroenteritis cases were caused by contaminated food; to account for uncertainty we used simulation techniques to estimate 90% credible intervals. we estimate that circa 2010, 4.1 million foodborne gastroenteritis cas ...201425340705
of microspheres and microbes: a double-hit strategy for cancer immunotherapy.efficient immunotherapy relies on the rapid generation of elevated amounts of cancer-specific t lymphocytes. we have recently demonstrated that both rapid and potent tumor-targeting immune responses can be induced with a heterologous prime-boost regimen consisting of poly-lactic co-glycolic acid (plga) microsphere-based immunization followed by listeria monocytogenes infection.201425340000
listeria monocytogenes-associated biliary tract infections: a study of 12 consecutive cases and review.at present, little is known regarding listeria monocytogenes-associated biliary tract infection, a rare form of listeriosis.in this article, we will study 12 culture-proven cases reported to the french national reference center for listeria from 1996 to 2013 and review the 8 previously published cases.twenty cases were studied: 17 cholecystitis, 2 cholangitis, and 1 biliary cyst infection. half were men with a median age of 69 years (32-85). comorbidities were present in 80%, including cirrhosis ...201425319439
novel mechanism of hemin capture by hbp2, the hemoglobin-binding hemophore from listeria monocytogenes.iron is an essential nutrient that is required for the growth of the bacterial pathogen listeria monocytogenes. in cell cultures, this microbe secretes hemin/hemoglobin-binding protein 2 (hbp2; lmo2185) protein, which has been proposed to function as a hemophore that scavenges heme from the environment. based on its primary sequence, hbp2 contains three near transporter (neat) domains of unknown function. here we show that each of these domains mediates high affinity binding to ferric heme (hemi ...201425315777
direct incorporation of the nkt-cell activator α-galactosylceramide into a recombinant listeria monocytogenes improves breast cancer vaccine efficacy.immune suppression in the tumour microenvironment remains a major limitation to successful immunotherapy of cancer. in the current study, we analysed whether the natural killer t cell-activating glycolipid α-galactosylceramide could overcome immune suppression and improve vaccination against metastatic breast cancer.201425314062
the listeria monocytogenes transposon tn6188 provides increased tolerance to various quaternary ammonium compounds and ethidium bromide.tolerance of the foodborne pathogen listeria monocytogenes to sublethal concentrations of disinfectants has been frequently reported. particularly, quaternary ammonium compounds (qacs) such as benzalkonium chloride (bc) are often used in disinfectants and also as antiseptics in food industry and hospitals. recently, we described tn6188, a novel transposon in l. monocytogenes harbouring the transporter qach, a molecular mechanism leading to increased tolerance to bc. in this study, we investigate ...201425312720
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