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odorant ligands for the co2 receptor in two anopheles vectors of malaria.exhaled co2 is an important host-seeking cue for anopheles mosquitoes, which is detected by a highly conserved heteromeric receptor consisting of three 7-transmembrane proteins gr22, gr23, and gr24. the co2 receptor neuron has been shown to also respond sensitively to a variety of odorants in aedes aegypti. the detection of co2 is important for upwind navigation and for enhancing the attraction to body heat as well as to skin odorants. the orthologs of the co2 receptor proteins are present in ma ...201930796292
associations between household environmental factors and immature mosquito abundance in quetzaltenango, guatemala.aedes aegypti-borne diseases are becoming major public health problems in tropical and sub-tropical regions. while socioeconomic status has been associated with larval mosquito abundance, the drivers or possible factors mediating this association, such as environmental factors, are yet to be identified. we examined possible associations between proximity to houses and roads and immature mosquito abundance, and assessed whether these factors and mosquito prevention measures mediated any associati ...201931870343
compatibility between leptolegnia chapmanii and diflubenzuron and neem oil for the control of aedes aegypti.leptolegnia chapmanii is pathogenic to mosquito larvae. the compatibility between l. chapmanii and two insect growth regulators (igr), diflubenzuron and neem oil, was evaluated. l. chapmanii was grown on culture media containing different concentrations of each igr. the mycelial growth was significantly reduced with the highest concentrations of igr (f6,98=268, p<0.05). however, the production of zoospores (f6,56=0.93, p>0.05) and the larval mortality of aedes aegypti (f6,56=0.95, p>0.05) were n ...201931866096
a non-conformational qsar study for plant-derived larvicides against zika aedes aegypti l. vector.a set of 263 plant-derived compounds with larvicidal activity against aedes aegypti l. (diptera: culicidae) vector is collected from the literature, and is studied by means of a non-conformational quantitative structure-activity relationships (qsar) approach. the balanced subsets method (bsm) is employed to split the complete dataset into training, validation and test sets. from 26,775 freely available molecular descriptors, the most relevant structural features of compounds affecting the bioact ...201931865579
age- and sex-related abc transporter expression in pyrethroid-susceptible and -resistant aedes aegypti.resistance mechanisms to synthetic insecticides often include point mutations and increased expression of genes encoding detoxification enzymes. since pyrethroids are the main adulticides used against aedes aegypti, which vectors pathogens such as zika virus, understanding resistance to this insecticide class is of significant relevance. we focused on adenosine triphosphate (atp)-binding cassette (abc) transporters in the pyrethroid-resistant puerto rico (pr) strain of ae. aegypti. we investigat ...201931862982
leptolegnia chapmanii como alternativa biológica para el control de aedes aegyptileptolegnia chapmanii is a facultative pathogen of many species of mosquitoes, among which species of the genus aedes, culex and anopheles stand out for their medical and sanitary importance. the potential of l. chapmanii as an alternative to control lies in its virulence, pathogenicity and specificity against the larval stages of mosquitoes, and because of its harmlessness to non-target species such as fish and amphibians, among others. the natural presence of l. chapmanii had been reported in ...201931860189
the first characterization of a cystatin and a cathepsin l-like peptidase from aedes aegypti and their possible role in denv infection by the modulation of apoptosis.recently, a salivary gland transcriptome study demonstrated that the transcripts of a putative cystatin gene (seqid aael013287; aacystatins) from aedes aegypti were increased in denv2-infected mosquitoes and that silencing of the aacystatin gene resulted in an increase in denv titres. in this work, aacystatin was biochemically characterized; the purified recombinant inhibitor was able to inhibit typical cysteine proteases with a ki in the nm range. pulldown assays using aag2 cell extracts identi ...201931857170
insecticide resistance in culex quinquefasciatus say, 1823 in brazil: a review.culex quinquefasciatus is a successful invasive species broadly distributed in subtropical regions, including brazil. it is an extremely annoying mosquito due to its nocturnal biting behavior, in high-density populations and it is a potential bridge between sylvatic arbovirus from birds to man in urban territories. herein, we present a review concerning the methods of chemical control employed against cx. quinquefasciatus in brazil since the 1950's and insecticide resistance data registered in t ...201931852489
deducing the role of virus genome-derived piwi-associated rnas in the mosquito-arbovirus arms race.the p-element-induced wimpy testis (piwi)-associated rna (pirna) pathway is known for its role in the protection of genome integrity in the germline of drosophila melanogaster by silencing transposable elements. the pirnas that target transposons originate from pirna clusters in transposon-rich regions of the drosophila genome and are processed by three piwi family proteins. in aedes aegypti and aedes albopictus mosquitoes, which are two of the most important vectors of arthropod-borne viruses ( ...201931850054
mechanisms of transport of h+, na+ and k+, across the distal gastric caecum of larval aedes aegypti.measured changes in ion fluxes, transepithelial potential (tep) and basolateral membrane potential (vb) in response to ion transporter inhibitors were used to assess the mechanisms of transport of h+, na+ and k+, across the distal gastric caecum of larval aedes aegypti, a vector of yellow fever. preparations were stimulated with 5-hydroxytryptamine (5-ht, 10-6 m) in order to maintain stable rates of h+, na+, and k+ transport across the distal caecum. transepithelial potential (tep), basolateral ...201931846613
effect of wolbachia walbb on a positive-sense rna negev-like virus: a novel virus persistently infecting aedes albopictus mosquitoes and cells.the aedes aegypti mosquito is the primary vector of several medically important arboviruses. the endosymbiotic bacterium, wolbachia pipientis, has emerged as a means of blocking transmission of arboviruses such as dengue and zika viruses. one wolbachia strain that has shown potential in field trials is walbb, a naturally occurring wolbachia strain of the asian tiger mosquito aedes albopictus. when transinfected into ae. aegypti, walbb exhibits strong virus inhibition. in addition to modulating a ...201931846415
antidengue potential of leaf extracts of pavetta tomentosa and tarenna asiatica (rubiaceae) against dengue virus and its vector aedes aegypti (diptera: culicidae).the aim of the present study was to screen the anti-dengue potential of crude leaf extracts of two plants from pavetta tomentosa and tarenna asiatica. for larvicidal assay, the acetone extract of both plants showed maximum effects, with the least lc50 and lc90 values (p. tomentosa (5.968 and 7.493 μg/ml) and t. asiatica (1.288 and 1.992 μg/ml)) and the same extract of both plants exhibited better pupicidal potency. the adulticidal activity of both plants (0-60 min interval periods) recorded best ...201931844692
author correction: cartography of odor chemicals in the dengue vector mosquito (aedes aegypti l., diptera/culicidae).an amendment to this paper has been published and can be accessed via a link at the top of the paper.201931844083
nano-emulsification enhances the larvicidal potential of the essential oil of siparuna guianensis (laurales: siparunaceae) against aedes (stegomyia) aegypti (diptera: culicidae).siparuna guianensis (laurales: siparunaceae) has a terpene-rich essential oil with great potential for larvicides. the poor water miscibility of their compounds makes nano-emulsions of great interest for novel bioactive systems, including for control of aedes aegypti (diptera: culicidae). this species is adapted to urban environments with important role in the epidemiology of some arboviruses such as dengue, chikungunya fever, zika, and urban yellow fever. the aim of the present study was to eva ...201931840745
could land cover influence aedes aegypti mosquito populations?aedes aegypti is mainly found in urban environments, where human activity guarantees the permanent availability of potential larval habitats. the present study aimed to test for a possible association between ae. aegypti infestation and land cover classes. from 23 february to 22 may 2015, immature mosquitoes (except eggs) were sampled in artificial containers identified in dwellings in córdoba city, argentina. the proportion of each land cover class was determined by spot-5 (satellites pour 1'ob ...201931840284
genome sequences of chikungunya virus isolates from an outbreak in southwest bangkok in 2018.an outbreak of chikungunya virus (chikv) infection occurred in southwest bangkok during the 2018 rainy season. the envelope glycoprotein e1 gene sequence of the infecting strain belonged to an east/central/south african lineage with alanine at residue 226. mutations in the predicted e1 (k211e) and e2 (v264a) proteins of chikv were identified in chikv-infected patients and in an aedes aegypti mosquito. analysis of the complete genome sequences showed marked differences from the strains causing pr ...201931834526
distribution of insecticide resistance and mechanisms involved in the arbovirus vector aedes aegypti in laos and implication for vector control.the yellow fever mosquito aedes aegypti is the major vector of dengue, yellow fever, zika, and chikungunya viruses. worldwide vector control is largely based on insecticide treatments but, unfortunately, vector control programs are facing operational challenges due to mosquitoes becoming resistant to commonly used insecticides. in southeast asia, resistance of ae. aegypti to chemical insecticides has been documented in several countries but no data regarding insecticide resistance has been repor ...201931830027
effects of larval diets and temperature regimes on life history traits, energy reserves and temperature tolerance of male aedes aegypti (diptera: culicidae): optimizing rearing techniques for the sterile insect programmes.producing high quality sterile males is vital in aedes aegypti rear-and-release birth control strategies. larval diets, rearing temperatures, and their interactions determine the accumulation rates of essential nutrients in larvae, but these factors have been understudied in relation to mass-rearing techniques for producing eminent males.201931823817
social capital is associated with lower mosquito vector indices: secondary analysis from a cluster randomised controlled trial of community mobilisation for dengue prevention in mexico.control of the aedes aegypti mosquito is central to reducing the risk of dengue, zika, chikungunya, and yellow fever. randomised controlled trials, including the camino verde trial in mexico and nicaragua, demonstrate the convincing impact of community mobilisation interventions on vector indices. these interventions might work through building social capital but little is known about the relationship between social capital and vector indices.201931823786
zika virus: a possible emerging threat for bangladesh!zika virus, a member of flaviviridae is the etiology of zika or zika fever or zika virus (zikv) disease characterized by mild symptoms similar to very mild form of dengue or chikungunya. the virus transmits through aedes mosquitoes, particularly by aedes aegypti. the most dangerous effect of zikv infection is the ability of the virus to cause microcephaly and congenital malformation to the newborn baby if the mother is infected. the neurological disorders including guillain-barré syndrome might ...201931819889
successive blood meals enhance virus dissemination within mosquitoes and increase transmission potential.the recent zika virus (zikv) and chikungunya virus epidemics highlight the explosive nature of arthropod-borne viruses (arboviruses) transmitted by aedes spp. mosquitoes1,2. vector competence and the extrinsic incubation period (eip) are two key entomological parameters used to assess the public health risk posed by arboviruses3. these are typically measured empirically by offering mosquitoes an infectious blood meal and temporally sampling mosquitoes to determine the infection and transmission ...201931819213
natural infection of aedes aegypti by chikungunya and dengue type 2 virus in a transition area of north-northeast brazil.dengue fever, chikungunya, and zika are diseases caused by viruses transmitted by aedes aegypti and aedes albopictus. in brazil, the number of human infections is high, but few studies are performed in mosquito vectors. this study aimed to investigate the presence of zika, dengue and chikungunya viruses in ae. aegypti and ae. albopictus from the municipalities of alto alegre, caxias, codó, and são mateus do maranhão, located in the state of maranhão, northeast brazil. the mosquitoes were collect ...201931817553
dengue virus reduces agpat1 expression to alter phospholipids and enhance infection in aedes aegypti.more than half of the world population is at risk of dengue virus (denv) infection because of the global distribution of its mosquito vectors. denv is an envelope virus that relies on host lipid membranes for its life-cycle. here, we characterized how denv hijacks the mosquito lipidome to identify targets for novel transmission-blocking interventions. to describe metabolic changes throughout the mosquito denv cycle, we deployed a liquid chromatography-high resolution mass spectrometry (lc-hrms) ...201931815960
genetic diversity of laboratory strains and implications for research: the case of aedes aegypti.the yellow fever mosquito (aedes aegypti), is the primary vector of dengue, zika, and chikungunya fever, among other arboviral diseases. it is also a popular laboratory model in vector biology due to its ease of rearing and manipulation in the lab. established laboratory strains have been used worldwide in thousands of studies for decades. laboratory evolution of reference strains and contamination among strains are potential severe problems that could dramatically change experimental outcomes a ...201931815934
glopid-r report on chikungunya, o'nyong-nyong and mayaro virus, part 5: entomological aspects.the glopid-r (global research collaboration for infectious disease preparedness) chikungunya (chikv), o'nyong-nyong (onnv) and mayaro virus (mayv) working group has been established to investigate natural history, epidemiology and clinical aspects of infection by these viruses. here, we present a report dedicated to entomological aspects of chikv, onnv and mayv. recent global expansion of chikungunya virus has been possible because chikv established a transmission cycle in urban settings using a ...201931812638
cryo-electron microscopy reveals that sperm modification coincides with female fertility in the mosquito aedes aegypti.manipulating mosquito reproduction is a promising approach to reducing mosquito populations and the burden of diseases they carry. a thorough understanding of reproductive processes is necessary to develop such strategies, but little is known about how sperm are processed and prepared for fertilization within female mosquitoes. by employing cryo-electron microscopy for the first time to study sperm of the mosquito aedes aegypti, we reveal that sperm shed their entire outer coat, the glycocalyx, ...201931811199
assessment of aedes aegypti (diptera: culicidae) males flight ability for sit application: effect of device design, duration of test, and male age.the sterile insect technique (sit) is a pest control method where large numbers of sterile males are released to induce sterility in wild populations. since a successful sit application depends on the released sterile males being competitive with wild males, standard quality control tests are a necessary component of any sit program. flight ability (ability to fly out from a device) is a reliable indicator of insect quality. based on previous studies, we developed four new tubular devices constr ...201931808821
malaria and dengue mosquito vectors from lao pdr show a lack of the rdl mutant allele responsible for cyclodiene insecticide resistance.the gamma-aminobutyric acid (gaba) receptor, rdl, plays important roles in neuronal signaling and is the target of highly effective insecticides. a mutation in rdl, commonly a296s, underlies resistance to several insecticides such as cyclodienes. even though the use of cyclodienes has been banned, the occurrence of mutations substituting a296 is notably high in mosquitoes from several countries. here, we report a survey investigating the prevalence of the rdl mutant allele in mosquitoes from lao ...201931807752
the consequences of brugia malayi infection on the flight and energy resources of aedes aegypti mosquitoes.evidence from experimental infection studies has shown that infected mosquitoes exhibit altered host-seeking behaviours, with suppression and activation of behaviours dependent on the parasite's development stage. the mechanisms are poorly characterised; however, infections can impact mosquito energy reserves, thereby influencing key life-history traits and behaviours. in addition, filarial infection is likely detrimental to flight due to damage caused by developing worms. this study aimed to ev ...201931804546
finding the sweet spot: what levels of larval mortality lead to compensation or overcompensation in adult production?extrinsic mortality impinging on negatively density-dependent populations can result in no change in the number of survivors (compensation) or an increase (overcompensation) by releasing the population from density-dependent effects on survivorship. the relationship between the level of extrinsic mortality (i.e., percentage of mortality) and the level and likelihood of overcompensation is theoretically important, but rarely investigated. we tested the hypothesis that overcompensation occurs belo ...201931803516
diterpenes and their derivatives as promising agents against dengue virus and dengue vectors: a literature-based review.dengue, a mosquito-borne viral disease, causes about 100 million cases of infection annually. it is a major public concern, and if left untreated or improperly diagnosed, may cause serious health problems or even death. historically, dengue has not considered priorities for pharmaceutical companies made the available treatment options. therefore, medicinal scientists are revealing new insights and enabling novel interventions and approaches to dengue prevention and control. diterpenes, a class o ...201931802573
[river boats as a means of expansion of aedes aegypti towards border zones of the peruvian amazon].to assess the entomological risk of aedes aegypti in boats traversing border river routes in loreto.201931800930
investigating male aedes aegypti (diptera: culicidae) attraction to different oviposition containers using various configurations of the sound gravid aedes trap.aedes aegypti (linnaeus), the primary vectors of the arboviruses dengue virus and zika virus, continue to expand their global distributions. in efforts to better control such species, several mosquito control programs are investigating the efficacy of rearing and releasing millions of altered male aedes throughout landscapes to reduce populations and disease transmission risk. unfortunately, little is known about ae. aegypti, especially male, dispersal behaviors within urban habitats. we deploye ...201931799614
drug repurposing of bromodomain inhibitors as potential novel therapeutic leads for lymphatic filariasis guided by multispecies transcriptomics.to better understand the transcriptomic interplay of organisms associated with lymphatic filariasis, we conducted multispecies transcriptome sequencing (rna-seq) on the filarial nematode brugia malayi, its wolbachia endosymbiont wbm, and its laboratory vector aedes aegypti across the entire b. malayi life cycle. in wbm, transcription of the noncoding 6s rna suggests that it may be a regulator of bacterial cell growth, as its transcript levels correlate with bacterial replication rates. for a. ae ...201931796568
cdte-gsh as luminescent biomarker for labeling the larvicidal action of wsmol lectin in aedes aegypti larvae.mosquito-borne arboviruses compromise human health worldwide. due to resistance to chemical insecticides, natural compounds have been studied to combat mosquitoes. previous works have demonstrated a larvicidal activity of the water-soluble moringa oleifera lectin (wsmol) against aedes aegypti, suggesting a mechanism of action based on the interaction between lectin and chitin present in the larvae's peritrophic matrix. in this work, it was investigated the wsmol activity against aedes aegypti la ...201931796242
inhibition of sodium-hydrogen antiport by antibodies to nha1 in brush border membrane vesicles from whole aedes aegypti larvae.the present research report describes na+/h+ antiport by brush border membrane vesicles isolated from whole larvae of aedes aegypti (aebbmvw). our hypothesis is that acid quenching of acridine orange by aebbmvw is predominantly mediated by na+/h+ antiport via the nha1 component of the aebbmvw in the absence of amino acids and atp. aenha1 is a na+/h+ antiporter that has been postulated to exchange na+ and h+ across the apical plasma membrane in posterior midgut of a. aegypti larvae. its principal ...201930392010
the combinatory effect of cyt1aa flexibility and specificity against dipteran larvae improves the toxicity of bacillus thuringensis kurstaki toxins.cyt1a98 is a novel cytolytic protein, from bupm98 bacillus thuringiensis strain, characterized by its synergistic activity with b. thuringiensis kurstaki toxins against lepidopteran larvae. in this study, we evidenced that cyt1a98 improves the toxicity of b. thuringiensis kurstaki toxins against aedes aegypti larvae. in fact, the strain bns3phtcyt1a98 exhibited a larvicidal activity of about 849-fold of that of bns3phtblue against a. aegypti. the molecular and biochemical characterizations, of c ...201930391590
bioassay and molecular study for detection of insecticide resistance dengue causing mosquito vectors.nowadays, dengue infection creates a major problem across the country. the vector species carrying dengue infection has progressively started to developed resistance against most of the currently used insecticides. hence, a study was carried out in dengue-endemic areas of assam and arunachal pradesh to find the current situation of insecticide susceptibility status of dengue vectors. based on the previous history of dengue incidence, aedes mosquitoes were collected from dibrugarh, kamrup, sivasa ...201930429402
autocidal gravid ovitrap incorporation with attractants for control of gravid and host-seeking aedes aegypti (diptera: culicidae).aedes aegypti linnaeus (diptera: culicidae) is a primary vector of multiple arboviruses including zika, dengue, chikungunya, and yellow fever. after incorporating bg lure or bg lure + octenol, the cdc's autocidal gravid ovitrap (ago) was more effective at collecting and controlling host-seeking female ae. aegypti than gravid female mosquitoes. the addition of octenol to the ago did not increase the number of female mosquitoes captured by the ago, compared with the ago alone. the ago baited with ...201930462293
monitoring insecticide resistance profiles of aedes aegypti (diptera: culicidae) in the sunda islands of indonesia based on diagnostic doses of larvicides.this study was conducted to monitor the susceptibility status of aedes aegypti (linnaeus) larvae in the sunda islands of indonesia against various organophosphates and organochlorines. larval bioassay was performed in accordance with the world health organization standard protocol. field-collected and reference strains of ae. aegypti larvae were tested against diagnostic doses of eight larvicides belonging to organophosphates and organochlorines, namely bromophos (0.050 mg/liter), chlopyrifos (0 ...201930462258
past, present and future of aedes aegypti in its south american southern distribution fringe: what do temperature and population tell us?aedes aegypti (diptera: culicidae) (linnaeus) is currently the major threat among arbovirus vectors in the americas. we examined its past, present, and future distribution patterns in the south american fringe in association with environmental and demographic variables at two spatial scales. we updated the database of the occurrence of ae. aegypti per locality and modelled by glmm the past occurrence (until 2000) and its expansion (2001-2017) as a function of air temperature, precipitation, alti ...201930458122
water channel activity of putative aquaporin-6 present in aedes aegypti.aquaporins (aqps) are integral membrane channels that facilitate the bidirectional transport of water and sometimes other small solutes across biological membranes. aqps are important in mediating environmental adaptations in mosquitoes and are considered as a novel target for the development of effective insecticides against mosquitoes. here, we expressed aedes aegypti aqp6 ( aaaqp6) in human embryonic kidney (hek) 293 cells and analyzed the water permeability by a conventional swelling assay, ...201930456765
saccharomyces cerevisiae (baker's yeast) as an interfering rna expression and delivery system.the broad application of rna interference for disease prevention is dependent upon the production of dsrna in an economically feasible, scalable, and sustainable fashion, as well as the identification of safe and effective methods for rna delivery. current research has sparked interest in the use of saccharomyces cerevisiae for these applications. this review examines the potential for commercial development of yeast interfering rna expression and delivery systems. s. cerevisiae is a genetic mod ...201930474529
ecotoxicity response of chlorpyrifos in aedes aegypti larvae and lactuca sativa seeds after uv/h2o2 and uvc oxidation.chlorpyrifos (cp) is an organophosphate pesticide widely used in agriculture known to cause neurological and immunological effects in addition to interfering in the reproduction and development of organisms. in this study, cp degradation by uv/h2o2 process and uvc radiation was investigated, and the ecotoxicity and phytotoxicity was evaluated using bioassays of aedes aegypti larvae and lactuca sativa seeds. cp degradation was monitored by hplc-dad, and kinetic parameters were calculated for all ...201930471582
coinfection of chikungunya and dengue viruses: a serological study from north western region of punjab, india.dengue and chikungunya (chik) infections appear to be increasing in all parts of india. aedes aegypti mosquitoes are common vectors for dengue virus (denv) and chik virus (chikv). in areas where both viruses cocirculate, they can be transmitted together. there are very few studies discussing the dengue-chik coinfection from punjab region of india. the present study was undertaken to study the clinical features of dengue-chik coinfection and compare with monoinfection.201930498319
volatile composition and biological activities of the leaf essential oil from zanthoxylum limoncello grown in oaxaca, méxico.zanthoxylum limoncello is a native plant from southern mexico which is used as a timber source, condiment and as a traditional medicine. herein, we report on the volatile content of the leaf essential oil and its biological activities. the annual essential oils (2015-2018) contained volatile organic compounds which exhibited a moderate growth inhibitory activity against h. pylori atcc 53504 (mic 121.4-139.7 μg ml-1 ), 26695 (mic 85.5-94.9 μg ml-1 ) and j99 (mic 94.7-110.4 μg ml-1 ). these hydrod ...201930512233
differential suppression of persistent insect specific viruses in trans-infected wmel and wmelpop-cla aedes-derived mosquito lines.wolbachia suppresses the replication of +ssrna viruses such as dengue and zika viruses in aedes aegypti mosquitoes. however, the range of viruses affected by this endosymbiont is yet to be explored. recently, novel insect-specific viruses (isvs) have been described from numerous mosquito species and mosquito-derived cell lines. cell-fusing agent virus (flaviviridae) and phasi charoen-like virus (bunyaviridae) persistently infect the ae. aegypti cell line aag2 which has been used for experimental ...201930503908
[new altitudinal registration of aedes aegypti in the region of piura, peru]. 201930517515
co-occurrence of v1016i and f1534c mutations in the voltage-gated sodium channel and resistance to pyrethroids in aedes aegypti (l.) from the colombian caribbean region.knockdown resistance is conferred primarily by non-synonymous mutations that reduce pyrethroids binding to voltage-gated sodium channels. in 2014, kdr mutation v1016i in aedes aegypti populations resistant to pyrethroids was reported for the first time in colombiα, in 2016 another kdr mutation, f1534c, and in 2018 the mutation v419l. nine populations of a. aegypti, previously characterized as being resistant to λ-cyhalothrin, deltamethrin, cyfluthrin and permethrin, were used for this study. gen ...201930520256
a gut commensal bacterium promotes mosquito permissiveness to arboviruses.mosquitoes are hematophagous vectors that can acquire human viruses in their intestinal tract. here, we define a mosquito gut commensal bacterium that promotes permissiveness to arboviruses. antibiotic depletion of gut bacteria impaired arboviral infection of a lab-adapted aedes aegypti mosquito strain. reconstitution of individual cultivable gut bacteria in antibiotic-treated mosquitoes identified serratia marcescens as a commensal bacterium critical for efficient arboviral acquisition. s. marc ...201930595552
a simple model to predict the potential abundance of aedes aegypti mosquitoes one month in advance.the mosquito aedes (stegomyia) aegypti (l.) is the primary vector of dengue, chikungunya, and zika viruses in the united states. surveillance for adult ae. aegypti is limited, hindering understanding of the mosquito's seasonal patterns and predictions of areas at elevated risk for autochthonous virus transmission. we developed a simple, intuitive empirical model that uses readily available temperature and humidity variables to predict environmental suitability for low, medium, or high potential ...201930594264
the tudor protein veneno assembles the ping-pong amplification complex that produces viral pirnas in aedes mosquitoes.piwi-interacting rnas (pirnas) comprise a class of small rnas best known for suppressing transposable elements in germline tissues. the vector mosquito aedes aegypti encodes seven piwi genes, four of which are somatically expressed. this somatic pirna pathway generates pirnas from viral rna during infection with cytoplasmic rna viruses through ping-pong amplification by the piwi proteins ago3 and piwi5. yet, additional insights into the molecular mechanisms mediating non-canonical pirna producti ...201930566680
container size alters the outcome of interspecific competition between aedes aegypti (diptera: culicidae) and aedes albopictus.aedes aegypti l. and aedes albopictus skuse co-occur in a variety of water-filled containers where they compete for resources. larvae of ae. albopictus skuse often outcompete those of ae. aegypti l., but variation in biotic and abiotic parameters can modify the outcome of this interspecific competition. we tested whether container size can alter the magnitude and direction of intra- and interspecific competition by rearing three ae. aegypti and ae. albopictus larval combinations (100:0, 50:50 an ...201930566608
bioactivity of wild carrot (daucus carota, apiaceae) essential oil against mosquito larvae.invasive alien plants wreak havoc on native ecosystems and using them as a source of biopesticides could improve their management. we examined the toxicity of essential oil of wild carrot (also known as 'queen anne's lace', daucus carota linnaeus), an aggressive invader throughout the united states, against aedes aegypti l., culex pipiens l., and culex restuans theobald larvae. comparisons were made between essential oil extracted from umbels of local populations of wild carrot versus a commerci ...201930566578
seroprevalence of zika virus among asymptomatic pregnant mothers and their newborns in the najran region of southwest saudi arabia.zika virus (zikv) is a teratogenic flavivirus that can cause microcephaly. its main vector, aedes aegypti, has been previ.ously identified in saudi arabia, but no zikv infection has yet been reported. nevertheless, the country is at risk from zikv because it receives many travelers throughout the year, including pilgrims from zikv-endemic countries.201930531174
mechanisms of pyrethroid resistance in aedes (stegomyia) aegypti from colombia.in colombia aedes (stegomyia) aegypti is the main vector of urban arboviruses such as dengue, chikungunya and zika. this urban mosquito has a well-established capacity to develop insecticide resistance to different types of insecticides (pyrethroids, organochlorides, organophosphates), using multiple resistance mechanisms. an understanding of ongoing resistance mechanisms is critical to determining the activities of vector control programs. in order to identify the biochemical and molecular mech ...201930552882
proteins, transcripts, and genetic architecture of seminal fluid and sperm in the mosquito aedes aegypti.the yellow fever mosquito, aedes aegypti,, transmits several viruses causative of serious diseases, including dengue, zika, and chikungunya. some proposed efforts to control this vector involve manipulating reproduction to suppress wild populations or to replace them with disease-resistant mosquitoes. the design of such strategies requires an intimate knowledge of reproductive processes, yet our basic understanding of reproductive genetics in this vector remains largely incomplete. to accelerate ...201930552291
distinctive regulatory properties of pyruvate kinase 1 from aedes aegypti mosquitoes.female aedes aegypti mosquitoes are vectors of arboviruses that cause diseases of public health significance. the discovery of new metabolic targets is crucial for improving mosquito control strategies. we recently demonstrated that glucose oxidation supports ammonia detoxification in a. aegypti. pyruvate kinase (pk, ec 2.7.1.40) catalyzes the last step of the glycolytic pathway. in most organisms, one or more allosteric effectors control pk activity. however, the kinetic properties and structur ...201930578824
production of tetravalent dengue virus envelope protein domain iii based antigens in lettuce chloroplasts and immunologic analysis for future oral vaccine development.dengue fever is a mosquito (aedes aegypti) -transmitted viral disease that is endemic in more than 125 countries around the world. there are four serotypes of the dengue virus (denv 1-4) and a safe and effective dengue vaccine must provide protection against all four serotypes. to date, the first vaccine, dengvaxia (cyd-tdv), is available after many decades' efforts, but only has moderate efficacy. more effective and affordable vaccines are hence required. plants offer promising vaccine producti ...201930578710
comparative efficacy of eucalyptus globulus (labill) hydrodistilled essential oil and temephos as mosquito larvicide.in this study eucalyptus globulus essential oil was tested on major mosquito species and toxicity was compared with temephos. mortality was calculated after 24 h and 48 h post treatment. in 24 h treatment essential oil show strong larvicidal activity with lc50 and lc90 values were 30.198ppm, 103.389ppm for anopheles stephensi, 13.578ppm, 106.755ppm for aedes aegypti; and, 7.469ppm, 32.454ppm for culex quinquefasciatus and 48 h post treatment lc50 and lc90 values were, 12.576, 49.380ppm for anoph ...201930623679
a novel entomological index, aedes aegypti breeding percentage, reveals the geographical spread of the dengue vector in singapore and serves as a spatial risk indicator for dengue.aedes aegypti is an efficient primary vector of dengue, and has a heterogeneous distribution in singapore. aedes albopictus, a poor vector of dengue, is native and ubiquitous on the island. though dengue risk follows the dispersal of ae. aegypti, the spatial distribution of the vector is often poorly characterized. here, based on the ubiquitous presence of ae. albopictus, we developed a novel entomological index, ae. aegypti breeding percentage (bp), to demonstrate the expansion of ae. aegypti i ...201930621762
matching the genetics of released and local aedes aegypti populations is critical to assure wolbachia invasion.traditional vector control approaches such as source reduction and insecticide spraying have limited effect on reducing aedes aegypti population. the endosymbiont wolbachia is pointed as a promising tool to mitigate arbovirus transmission and has been deployed worldwide. models predict a rapid increase on the frequency of wolbachia-positive ae. aegypti mosquitoes in local settings, supported by cytoplasmic incompatibility (ci) and high maternal transmission rate associated with the wmelbr strain ...201930620733
identification and characterization of a mosquito-specific eggshell organizing factor in aedes aegypti mosquitoes.mosquito-borne diseases are responsible for several million human deaths annually around the world. one approach to controlling mosquito populations is to disrupt molecular processes or antagonize novel metabolic targets required for the production of viable eggs. to this end, we focused our efforts on identifying proteins required for completion of embryonic development that are mosquito selective and represent potential targets for vector control. we performed bioinformatic analyses to identif ...201930620728
density-dependent enhanced replication of a densovirus in wolbachia-infected aedes cells is associated with production of pirnas and higher virus-derived sirnas.the endosymbiotic bacterium wolbachia pipientis has been shown to restrict a range of rna viruses in drosophila melanogaster and transinfected dengue mosquito, aedes aegypti. here, we show that wolbachia infection enhances replication of aedes albopictus densovirus (aaldnv-1), a single stranded dna virus, in aedes cell lines in a density-dependent manner. analysis of previously produced small rnas of aag2 cells showed that wolbachia-infected cells produced greater absolute abundance of virus-der ...201930583288
the aedes aegypti imd pathway is a critical component of the mosquito antifungal immune response.successful infection of the insect body by entomopathogenic fungi is the result of complex molecular interactions between the host and the invading pathogenic fungi. the mosquito antifungal response is multifaceted and is regulated in part by the toll and jak-stat pathways. here, we assessed the role of the imd pathway in the mosquito ae. aegypti antifungal immune response when challenged with one of two entomopathogenic fungi, beauveria bassiana and isaria javanica. imd pathway components of th ...201930582948
green synthesis of silver-nanoparticles from annona reticulata leaves aqueous extract and its mosquito larvicidal and anti-microbial activity on human pathogens.silver nanoparticles play a important role in controlling mosquito population as well as multi drug resistant pathogens without causing much harm to humans. in the present study was focused on green synthesis of silver nanoparticles against dengue causing vector (aedes aegypti) and pathogens affecting humans. the synthesized silver nanoparticle was confirmed using uv- absorption spectrum range obtained at 416 nm, xrd, ftir and hr-tem analysis were used to determine the silver nanoparticle morpho ...201930581768
adaptation of aedes aegypti to salinity: characterized by larger anal papillae in larvae. 201930618451
epidemic potential of zika virus in australia: implications for blood transfusion safety.zika virus (zikv) is transfusion-transmissible. in australia the primary vector, aedes aegypti, is established in the north-east, such that local transmission is possible following importation of an index case, which has the potential to impact on blood transfusion safety and public health. we estimated the basic reproduction number (r 0 ) to model the epidemic potential of zikv in australian locations, compared this with the ecologically similar dengue viruses (denv), and examined possible impl ...201930618208
larvicidal and pupicidal activities of eco-friendly phenolic lipid products from anacardium occidentale nutshell against arbovirus vectors.aedes aegypti and culex quinquefasciatus are vectors of diseases that constitute public health problems. the discovery of products capable of inhibiting their development which are less harmful to the environment would have a huge impact on vector control. here, natural cashew nut shell liquid (cnsl), technical cnsl, anacardic acid, cardanol, and cardol were isolated from anacardium occidentale and evaluated for larvicidal and pupicidal activity against ae. aegypti and cx. quinquefasciatus under ...201930610586
assessment of combined tools and strategies for aedes aegypti control with low environmental impact.the control of the mosquito vector aedes aegypti l. (diptera: culicidae) is the main action against dengue, chikungunya, and zika. the excessive use of conventional insecticides has promoted the development of other control methods and strategies with lower environmental impact. we evaluated the effectiveness of applying triflumuron 1 ppm and emptying water-filled containers in a field trial in temperate argentina. both control methods were implemented either individually or combined and regular ...201930607607
alternative strategies for mosquito-borne arbovirus control.mosquito-borne viruses-such as zika, chikungunya, dengue fever, and yellow fever, among others-are of global importance. although vaccine development for prevention of mosquito-borne arbovirus infections has been a focus, mitigation strategies continue to rely on vector control. however, vector control has failed to prevent recent epidemics and arrest expanding geographic distribution of key arboviruses, such as dengue. as a consequence, there has been increasing necessity to further optimize cu ...201930605475
laboratory evaluation of repellency of traditional czech homemade repellents against aedes aegypti.in the czech republic, autochtonous transmission of mosquito borne diseases is not common; however, the need for personal protection should not be underestimated. many people still rely on homemade repellents utilizing recipes based on local folk wisdom that are published annually in local czech media. despite minimal disease risk, nuisance biting and potential allergic responses make it essential to evaluate the chemical composition, effect, and duration of four homemade repellents often used a ...201930602280
igg1 and igg4 antibodies against aedes aegypti salivary proteins and risk for dengue infections.dengue virus (denv) is an arbovirus responsible for a significant number of deaths in latin america. this virus is transmitted through the bite of aedes aegypti, the main mosquito vector, and ae. albopictus. during blood uptake, the mosquito injects its saliva into the host to facilitate the feeding process. mosquito saliva contains potent immunogens capable of inducing antibody production directly related to mosquito bite exposure intensity and disease risk. in this study, we first determined t ...201930601814
fine-scale spatial and temporal dynamics of kdr haplotypes in aedes aegypti from mexico.as resistance to insecticides increases in disease vectors, it has become exceedingly important to monitor populations for susceptibility. most studies of field populations of aedes aegypti have largely characterized resistance patterns at the spatial scale of the city or country, which may not be completely informative given that insecticide application occurs at the scale of the house or city block. phenotypic resistance to pyrethroids dominates in ae. aegypti, and it has been partially explai ...201930626410
microbial exopolymer-capped selenium nanowires - towards new antibacterial, antibiofilm and arbovirus vector larvicides?arboviral diseases and microbial pathogens resistant to commercially available drugs are on the rise. herein, a facile microbial-based approach was developed to synthesize selenium nanowires (se nws) using microbial exopolymer (mep) extracted from the bacillus licheniformis (probiotic bacteria). mep-se nws were characterized using uv-visible, xrd, ftir, hr-tem, fe-sem and edx. an uv-visible peak was detected at 330 nm while xrd spectrum data pointed out the crystalline nature of mep-se nws. ftir ...201930685584
complete nmr chemical shift assignments of odorant binding protein 22 from the yellow fever mosquito, aedes aegypti, bound to arachidonic acid.aedes aegypti mosquitoes are the vector for transmission of dengue, zika and chikungunya viruses. these mosquitos feed exclusively on human hosts for a blood meal. previous studies have established that dengue virus infection of the mosquito results in increased expression of the odorant binding proteins 22 and 10 within the mosquito salivary gland and silencing of these genes dramatically reduces blood-feeding behaviors. odorant binding proteins are implicated in modulating the chemosensory per ...201930684234
toxicity and possible mechanisms of action of honokiol from magnolia denudata seeds against four mosquito species.this study was performed to determine the toxicity and possible mechanism of the larvicidal action of honokiol, extracted from magnolia denudata seeds, and its 10 related compounds against third-instar larvae of insecticide-susceptible culex pipiens pallens, aedes aegypti, and aedes albopictus and anopheles sinensis resistant to deltamethrin and temephos. honokiol (lc50, 6.13-7.37 mg/l) was highly effective against larvae of all of the four mosquito species, although the toxicity of the compound ...201930674912
chemical composition, aedes mosquito larvicidal activity, and repellent activity against triatoma rubrofasciata of severinia monophylla leaf essential oil.aedes aegypti and ae. albopictus are key vectors in the spread of arboviruses such as dengue, chikungunya, yellow fever, and zika. triatoma rubrofasciata is an "assassin bug" whose populations and association with humans have dramatically increased and may represent a serious health concern. control of insect vectors is a logical course of action to prevent the spread of these insect-borne infections. this work presents the leaf essential oil composition, mosquito larvicidal activities, and inse ...201930671730
glycosaminoglycan compositional analysis of relevant tissues in zika virus pathogenesis and in vitro evaluation of heparin as an antiviral against zika virus infection.zika virus (zikv) is an enveloped rna virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women. previously, we established that zikv-ep (envelope protein) binds to human placental chondroitin sulfate (cs), suggesting that cs may be a potential host cell surface receptor in zikv pathogenesis. in this study, we further characterized the gag disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in zikv pathoge ...201930698412
presence of the point mutations val1016gly in the voltage-gated sodium channel detected in a single mosquito from panama.in panama, arboviroses such as dengue fever, and more recently chikungunya fever and zika disease, are transmitted by aedes aegypti and aedes albopictus. their control is based on the elimination of breeding sites and fogging with pyrethroid insecticides. however, one of the significant issues derived from the prolonged use of pyrethroid insecticide is the development of resistance mechanisms, such as knockdown resistance or kdr. the objective of this study was to evaluate the presence of kdr mu ...201930691518
identification of potential inhibitors from pyriproxyfen with insecticidal activity by virtual screening.aedes aegypti is the main vector of dengue fever transmission, yellow fever, zika, and chikungunya in tropical and subtropical regions and it is considered to cause health risks to millions of people in the world. in this study, we search to obtain new molecules with insecticidal potential against ae. aegypti via virtual screening. pyriproxyfen was chosen as a template compound to search molecules in the database zinc_natural_stock (znst) with structural similarity using rocs (rapid overlay of c ...201930691028
factors affecting short-range host-seeking for the yellow fever mosquito (diptera: culicidae).understanding short-range cues (e.g., host odorants, heat, moisture) of host-seeking female aedes aegypti l. is very important for attempts to reduce mosquito bites, to complement current control strategies, and to develop potential spatial repellents. we investigated behavior under semi-field conditions utilizing a new portable uni-port taxis box with a caged chicken host. the combined influences of airflow regimes (0, 1.5, 3, 5, and 6 m/s), distance from host odor (10, 50, 100 cm), host-odor c ...201930689913
predation on eggs of aedes aegypti (diptera: culicidae): temporal dynamics and identification of potential predators during the winter season in a temperate region.in temperate regions, the populations of aedes aegypti (l.) remain in the egg stage during the cold season. the ability of these eggs to survive until the next favorable season is affected by several mortality factors, including the action of predators. in the present study, we analyzed the temporal dynamics of the loss of eggs and identified predators of ae. aegypti eggs along the unfavorable season in a temperate region of argentina. to this end, eggs were exposed in field conditions in pitfal ...201930689912
rapid loss of resistance to satyrization in invasive mosquitoes and the effects of age on interspecific mating frequency.in several areas where aedes aegypti (l.) (diptera: culicidae) and aedes albopictus (skuse) (diptera: culicidae) have come in contact following successful invasions, ae. aegypti have been rapidly displaced by ae. albopictus. recent work has confirmed that mating interference, in the form of satyrization, is likely a driving factor in these competitive displacements. however, in sites of sympatry, ae. aegypti females evolve resistance to satyrization, and in the laboratory, satyrization-susceptib ...201930668807
florida aedes aegypti (diptera: culicidae) and aedes albopictus vector competency for zika virus.zika virus (zikv) first spread into brazil in 2013 and is now present throughout the americas. in 2016, florida witnessed the beginnings of local zikv transmission. no reports of local transmission have been reported for 2018; however, travel related cases continue to be reported. recurrence of local transmission in the united states is a major public health risk in florida where vectors aedes aegypti (diptera: culicidae, linnaeus) and aedes albopictus (diptera: culicidae, skuse) are abundant an ...201930668772
infection pattern of mayaro virus in aedes aegypti (diptera: culicidae) and transmission potential of the virus in mixed infections with chikungunya virus.mayaro virus (mayv; togaviridae; alphavirus) has drawn increasing attention as an arthropod-borne virus with potential to cause outbreaks among the human populations of the western hemisphere. in the tropical regions of central and south america, the virus exists in sylvatic cycles between mosquitoes and primate reservoirs such as marmosets. although forest-dwelling mosquitoes are regarded as important vectors for mayv, it has been shown previously that the virus can infect and potentially be tr ...201930668762
response of aedes aegypti and aedes albopictus (diptera: culicidae) survival, life history, and population growth to oak leaf and acorn detritus.aedes albopictus (skuse) (diptera: culicidae) and aedes aegypti (l.) (diptera: culicidae) are invasive container mosquitoes that are of potential medical importance in the southern united states. seeds (acorns) and leaves from oak trees can contribute seasonally to the detritus of larval container habitats. herein, we examined the effect of acorns and leaves from the southern live oak (quercus virginiana mill.), which has a concomitant range with these mosquitoes, on the population performance o ...201930668756
spermathecal filling in aedes aegypti and aedes albopictus: effects of female and male body sizes and species.the mosquitoes aedes aegypti (l.) (diptera: culicidae) and aedes albopictus (skuse) (diptera: culicidae) are ecologically similar species that have evolved independently in their native ranges, ae. aegypti in africa and ae. albopictus in asia. where their invasive ranges overlap, interspecific mating is facilitated by both species swarming to mate around bloodmeal hosts during daylight. here, we test hypotheses to account for variation in spermathecal filling in females of ae. aegypti and ae. al ...201930668724
arbovirus coinfection and co-transmission: a neglected public health concern?epidemiological synergy between outbreaks of viruses transmitted by aedes aegypti mosquitoes, such as chikungunya, dengue, and zika viruses, has resulted in coinfection of humans with multiple viruses. despite the potential impact on public health, we know only little about the occurrence and consequences of such coinfections. here, we review the impact of coinfection on clinical disease in humans, discuss the possibility for co-transmission from mosquito to human, and describe a role for modeli ...201930668574
entomological and sociobehavioral components of heartworm (dirofilaria immitis) infection in two florida communities with a high or low prevalence of dogs with heartworm infection.objective to assess knowledge, attitudes, and practices regarding heartworms (dirofilaria immitis) and mosquito vectors among residents (dog owners and non-dog owners) in 2 neighborhoods in florida and to perform entomological surveys of mosquito species in these neighborhoods and identify mosquito species infected with heartworm. design cross-sectional study. sample 2,572 mosquitoes and 96 residents of 2 northern florida communities. procedures a 32-item questionnaire was orally administered to ...201930668298
effect of meteorological factors on the seasonal prevalence of dengue vectors in upland hilly and lowland terai regions of nepal.the expansion of dengue vectors from lowland plains to the upland hilly regions of nepal suggests the likelihood of increased risk of dengue. our objective was to assess the effects of meteorological variables on vector indices and populations of dengue vectors in two different ecological regions of nepal. an entomological survey was conducted in kathmandu and lalitpur (upland) and chitwan (lowland) of nepal in three different seasons from july 2015 to may 2016. the effect of meteorological vari ...201930658693
aedes aegypti larvae treated with spinosad produce adults with damaged midgut and reduced fecundity.the mosquito aedes aegypti is the main vector of dengue, chikungunya, zika, and yellow fever viruses, which are responsible for high human morbidity and mortality. the fight against these pathogens is mainly based on the control of the insect vector with the use of insecticides. among insecticides, spinosad bioinsecticide is efficient against a. aegypti larvae and may be an alternative for vector control. here, we investigate the sublethal effects of spinosad during midgut metamorphosis of a. ae ...201930654260
a role for the insulin receptor in the suppression of dengue virus and zika virus in wolbachia-infected mosquito cells.wolbachia-infected mosquitoes are refractory to super-infection with arthropod-borne pathogens, but the role of host cell signaling proteins in pathogen-blocking mechanisms remains to be elucidated. here, we use an antibody microarray approach to provide a comprehensive picture of the signaling response of aedes aegypti-derived cells to wolbachia. this approach identifies the host cell insulin receptor as being downregulated by the bacterium. furthermore, sirna-mediated knockdown and treatment w ...201930650347
extremely long viability of aedes aegypti (diptera: culicidae) eggs stored under normal room condition.the viability of aedes aegypti (linnaeus) eggs was determined by storing the eggs for long period of up to 1,889 d. the viability of eggs declined over time from 88.54 to 4.89% for the storage period of 1-1,883 d (5.15 yr). the percentage of collapsed eggs was inversely proportional to the hatching rate of eggs. in the first day of storage, the eggs remained healthy and intact, and except a few, the eggs were deformed and boat shaped with cleft due to inward shrinkage during long storage of 1,88 ...201930649399
cross-generational effects of heat stress on fitness and wolbachia density in aedes aegypti mosquitoes.aedes aegypti mosquitoes infected with wolbachia symbionts are now being released into the field to control the spread of pathogenic human arboviruses. wolbachia can spread throughout vector populations by inducing cytoplasmic incompatibility and can reduce disease transmission by interfering with virus replication. the success of this strategy depends on the effects of wolbachia on mosquito fitness and the stability of wolbachia infections across generations. wolbachia infections are vulnerable ...201930642130
application of convolutional neural networks for classification of adult mosquitoes in the field.dengue, chikungunya and zika are arboviruses transmitted by mosquitos of the genus aedes and have caused several outbreaks in world over the past ten years. morphological identification of mosquitos is currently restricted due to the small number of adequately trained professionals. we implemented a computational model based on a convolutional neural network (cnn) to extract features from mosquito images to identify adult mosquitoes from the species aedes aegypti, aedes albopictus and culex quin ...201930640961
genome-wide transcriptome profiling reveals genes associated with meiotic drive system of aedes aegypti.aedes aegypti is an important mosquito vector of several arboviruses, including dengue, yellow fever, zika, and chikungunya, which cause significant human morbidity and mortality globally. in certain populations of this mosquito, a native meiotic drive system causes abnormal spermatogenesis that results in highly male-biased progenies from some matings. although the basic genetics and cytogenetics of the drive mechanism were elucidated, very little is known on a transcriptome level about how the ...201930634571
aspergillus flavus (link) toxins reduces the fitness of dengue vector aedes aegypti (linn.) and their non-target toxicity against aquatic predator.mosquito that accountable for dispersal of dengue fever is aedes aegypti linn. and considered to be a chief vector for dengue especially in south asian countries. aspergillus flavus is considered to be wild growing green yellow colonies and synthesis highly regulating aflatoxins (b1, b2, g1 and g2) as a secondary metabolite. mycotoxins of a. flavus showed its efficacy against iii and iv instars of ae. aegypti with more than 90% mortality at the prominent dosage of 2 × 108 conidia/ml. the proxima ...201930633984
impact of sugar composition on meal distribution, longevity, and insecticide toxicity in aedes aegypti.toxic sugar baits (tsbs) are an inexpensive and field-applicable approach to deliver a variety of insecticides to sugar-seeking mosquitoes. we reasoned that carbohydrate chemistry could alter the performance and efficacy of tsbs. in this study, the uptake, distribution, and survival of female aedes aegypti provided with twelve different aqueous sugar meals was recorded. sucrose, a standard control sugar used in mosquito rearing, is always diverted to the ventral diverticulum upon ingestion; but ...201930633897
honeysuckle essential oil as a potential source of ecofriendly larvicides for mosquito control.some plant essential oils have insecticidal properties against mosquitoes and can be harnessed as ecofriendly tools for mosquito control. we conducted bioassays to determine the toxicity of italian honeysuckle (lonicera caprifolium) essential oil and its fractions against larvae of the yellow fever mosquito, aedes aegypti.201930632272
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