Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
supersensitive odorant receptor underscores pleiotropic roles of indoles in mosquito ecology.mosquitoes exhibit highly diverse and fast evolving odorant receptors (ors). the indole-sensitive or gene clade, comprised of or2 and or10 is a notable exception on account of its conservation in both mosquito subfamilies. this group of paralogous genes exhibits a complex developmental expression pattern in aedes aegypti: aaegor2 is expressed in both adults and larvae, aaegor10 is adult-specific and a third member named aaegor9 is larva-specific. or2 and or10 have been deorphanized and are selec ...201830733668
current status of aedes aegypti insecticide resistance development from banjarmasin, kalimantan, indonesia.aedes aegypti represents the principal vector of many arthropod-borne diseases in tropical areas worldwide. since mosquito control strategies are mainly based on use of insecticides, resistance development can be expected to occur in frequently exposed ae. aegypti populations. surveillance on resistance development as well as testing of insecticide susceptibility is therefore mandatory and needs further attention by national/international public health authorities. in accordance, we here conduct ...201830671445
insecticidal and repellent properties of novel trifluoromethylphenyl amides ii.this project focused on the design, synthesis, and testing of trifluoromethylphenyl amides (tfmpas) as potential mosquitocides and repellents. fourteen compounds were evaluated for toxicity against larvae and adults of aedes aegypti. several compounds were toxic against aedes aegypti (larval, adult and feeding bioassays) and drosophila melanogaster (glass-surface contact assay), but were much less toxic than fipronil, with toxicity ratios ranging from 100-fold in the larval assay to 100,000-fold ...201830704711
toxicity, mode of action, and synergist potential of flonicamid against mosquitoes.the present study focused on the toxicity of the aphid anti-feedant flonicamid and its main metabolite, 4-trifluoromethylnicotinamide (tfna-am) to aedes aegypti and anopheles gambiae mosquitoes. the compounds were toxic to both species via topical application, resulting in un-coordinated locomotion and leg splaying, with a favorable an. gambiae ld50 value of 35 ng/mg for tfna-am, but no significant lethality to ae. aegypti at 10 μg/female. there was mild cross resistance in the akron-kdr (akdr) ...201830704710
use of chemical probes to explore the toxicological potential of the k+/cl- cotransporter (kcc) as a novel insecticide target to control the primary vector of dengue and zika virus, aedes aegypti.the majority of commercialized insecticides target the insect nervous system and therefore, neural proteins are well-validated targets for insecticide development. considering that only a few neural targets are exploited for insecticidal action and the development of insecticide resistance has reduced the efficacy of current insecticidal classes, we sought to test the toxicological potential of the potassium-chloride cotransporter (kcc). in mammals, kcc proteins have seminal roles in shaping gab ...201830704707
zebrafish embryo toxicity of a binary mixture of pyrethroid insecticides: d-tetramethrin and cyphenothrin.pesguard fg161™, a mixture of d-tetramethrin and cyphenothrin (1:3 ratio), is extensively used to achieve rapid control of adult dengue vector, aedes aegypti, during the disease outbreaks. both d-tetramethrin and cyphenothrin are synthetic pyrethroids that are known to have adverse effects on non-mammalian organisms such as fish. the present study intended to use zebrafish embryo toxicity model to investigate the toxic effect of the above binary mixture on fish. particularly, zebrafish embryo to ...201830687404
scaled deployment of wolbachia to protect the community from dengue and other  aedes transmitted arboviruses.background: a number of new technologies are under development for the control of mosquito transmitted viruses, such as dengue, chikungunya and zika that all require the release of modified mosquitoes into the environment. none of these technologies has been able to demonstrate evidence that they can be implemented at a scale beyond small pilots. here we report the first successful citywide scaled deployment of wolbachia in the northern australian city of townsville. methods: the wmel strain of ...201830596205
larvicidal potential of five selected dragonfly nymphs in sri lanka over aedes aegypti (linnaeus) larvae under laboratory settings.limitations in breeding source reduction practices, development of insecticide resistance in mosquitoes, and ill effects of chemical controlling methods on human and ecosystem health have motivated sri lankan authorities working for dengue control to seek for alternative, ecofriendly, and sustainable approaches for controlling of aedes vectors, to manage dengue epidemics. the present study attempted to investigate the predation efficiency of locally available dragonfly nymphs over aedes aegypti ...201830627580
zika virus infection produces a reduction on aedes aegypti lifespan but no effects on mosquito fecundity and oviposition success.a zika virus (zikv) pandemic started soon after the first autochthonous cases in latin america. although aedes aegypti is pointed as the primary vector in latin america, little is known about the fitness cost due to zikv infection. we investigated the effects of zikv infection on the life-history traits of ae. aegypti females collected in three districts of rio de janeiro, brazil (barra, deodoro, and porto), equidistant ~25 km each other. aedes aegypti mosquitoes were classified into infected (a ...201830619118
long-term exposure of aedes aegypti to bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agents.bacillus thuringiensis svar. israelensis (bti) is an effective and safe biolarvicide to control aedes aegypti. its mode of action based on four protoxins disfavors resistance; however, control in endemic areas that display high mosquito infestation throughout the year requires continuous larvicide applications, which imposes a strong selection pressure. therefore, this study aimed to investigate the effects of an intensive bti exposure on an ae. aegypti strain (recbti), regarding its susceptibil ...201830594214
biochemical effects of petroselinum crispum (umbellifereae) essential oil on the pyrethroid resistant strains of aedes aegypti (diptera: culicidae).in ongoing screening research for edible plants, petroselinum crispum essential oil was considered as a potential bioinsecticide with proven antimosquito activity against both the pyrethroid susceptible and resistant strains of aedes aegypti. due to the comparative mosquitocidal efficacy on these mosquitoes, this plant essential oil is promoted as an attractive candidate for further study in monitoring resistance of mosquito vectors. therefore, the aim of this study was to evaluate the impact of ...201830586929
a comparative study of energy contents in mosquito vectors of malaria and dengue prevailing in jodhpur city (thar desert) of rajasthan state, india.transmission of malaria and dengue in the desert part of india is mainly caused by anopheles stephensi and aedes aegypti respectively. the maintenance and transmission of the pathogens that cause malaria and dengue are dependent on the physiology of the mosquito vectors. we aimed to measure the energy contents in the mosquitoes transmitting malaria and dengue in the desert part of the country.201830584552
combined sterile insect technique and incompatible insect technique: sex separation and quality of sterile aedes aegypti male mosquitoes released in a pilot population suppression trial in thailand.the sterile insect technique (sit), which is based on irradiation-induced sterility, and incompatible insect technique (iit), which is based on wolbachia-induced cytoplasmic incompatibility (a kind of male sterility), have been used as alternative methods to reduce mosquito vector populations. both methods require the release of males to reduce fertile females and suppress the number of natural populations. different techniques of sex separation to obtain only males have been investigated previo ...201830583749
molecular tools to create new strains for mosquito sexing and vector control.vector control programs based on population reduction by matings with mass-released sterile insects require the release of only male mosquitoes, as the release of females, even if sterile, would increase the number of biting and potentially disease-transmitting individuals. while small-scale releases demonstrated the applicability of sterile males releases to control the yellow fever mosquito aedes aegypti, large-scale programs for mosquitoes are currently prevented by the lack of efficient sexi ...201830583736
functional analysis of the promoter of an early zygotic gene klc2 in aedes aegypti.aedes aegypti is an important mosquito vector that transmits arboviruses that cause devastating diseases including zika, dengue fever, yellow fever and chikungunya. improved understanding of gene regulation in the early development of ae. aegypti will facilitate genetic studies and help the development of novel control strategies of this important disease vector.201830583735
sex determination and aedes population control.the global economic cost of aedes-borne diseases, such as dengue, is estimated to be in the billions of dollars annually. in this scenario, a sustained vector control strategy is the only alternative to control dengue, as well as other diseases transmitted by aedes, including zika and chikungunya. the use of transgenic mosquitoes is a promising weapon in the improvement of approaches currently applied in aedes aegypti control. field trials using genetically modified mosquitoes for population con ...201830583731
pure early zygotic genes in the asian malaria mosquito anopheles stephensi.the asian malaria mosquito, anopheles stephensi, is a major urban malaria vector in the middle east and on the indian subcontinent. early zygotic transcription, which marks the maternal-to-zygotic transition, has not been systematically studied in an. stephensi or any other anopheles mosquitoes. improved understanding of early embryonic gene expression in an. stephensi will facilitate genetic and evolutionary studies and help with the development of novel control strategies for this important di ...201830583723
exploring the potential of computer vision analysis of pupae size dimorphism for adaptive sex sorting systems of various vector mosquito species.several mosquito population suppression strategies based on the rearing and release of sterile males have provided promising results. however, the lack of an efficient male selection method has hampered the expansion of these approaches into large-scale operational programmes. currently, most of these programmes targeting aedes mosquitoes rely on sorting methods based on the sexual size dimorphism (ssd) at the pupal stage. the currently available sorting methods have not been developed based on ...201830583722
geographical distribution of aedes aegypti and aedes albopictus (diptera: culicidae) and genetic diversity of invading population of ae. albopictus in the republic of the congo.background: the arbovirus vector, aedes albopictus, originating from asia, has recently invaded african countries, including the republic of the congo, where it was associated with a chikungunya outbreak. up until now, little was known about its distribution in relation to the native aedes aegypti and how the invasion will modify the epidemiology of arboviral diseases. here, we assessed the current distribution of ae. albopictus and ae. aegypti in the republic of the congo and explored the genet ...201830175244
dengue outbreaks and the geographic distribution of dengue vectors in taiwan: a 20-year epidemiological analysis.dengue fever is an important mosquito-borne viral infectious disease that mostly occurs in tropical and subtropical areas of the world. according to epidemiological data from the center for disease control of taiwan, more than 98.62% of outbreaks of indigenous total dengue cases were reported in the southern part of taiwan. southern taiwan is an aggregate area encompassing tainan, kaohsiung, and pingtung, all of which are located below the tropic of cancer (23º35'n). with a few exceptions, dengu ...201830580791
dengue in taiwan: pointing the finger at aedes aegypti.in this issue of the biomedical journal we explore the history of dengue infection in taiwan and what current trends have to say about the vector responsible for transmitting the disease on the island. we focus on original research reporting the development of a new perfusion bioreactor to engineer bone from human cord blood stem cells. finally, we look at trends in osteoporosis in taiwan and how they highlight the success of public health campaigns.201830580790
soluble expression of recombinant midgut zymogen (native propeptide) proteases from the aedes aegypti mosquito utilizing e. coli as a host.studying proteins and enzymes involved in important biological processes in the aedes aegypti mosquito is limited by the quantity that can be directly isolated from the mosquito. adding to this difficulty, digestive enzymes (midgut proteases) involved in metabolizing blood meal proteins require a more oxidizing environment to allow proper folding of disulfide bonds. therefore, recombinant techniques to express foreign proteins in escherichia coli prove to be effective in producing milligram quan ...201830563449
simultaneous detection of different zika virus lineages via molecular computation in a point-of-care assay.we have developed a generalizable "smart molecular diagnostic" capable of accurate point-of-care (poc) detection of variable nucleic acid targets. our isothermal assay relies on multiplex execution of four loop-mediated isothermal amplification reactions, with primers that are degenerate and redundant, thereby increasing the breadth of targets while reducing the probability of amplification failure. an easy-to-read visual answer is computed directly by a multi-input boolean or logic gate (gate o ...201830558136
larval exposure to the bacterial insecticide bti enhances dengue virus susceptibility of adult aedes aegypti mosquitoes.understanding the interactions between pathogens sharing the same host can be complicated for holometabolous animals when larval and adult stages are exposed to distinct pathogens. in medically important insect vectors, the effect of pathogen exposure at the larval stage may influence susceptibility to human pathogens at the adult stage. we addressed this hypothesis in the mosquito aedes aegypti, a major vector of arthropod-borne viruses (arboviruses), such as the dengue virus (denv) and the chi ...201830558130
evolution of maternal and zygotic mrna complements in the early drosophila embryo.the earliest stages of animal development are controlled by maternally deposited mrna transcripts and proteins. once the zygote is able to transcribe its own genome, maternal transcripts are degraded, in a tightly regulated process known as the maternal to zygotic transition (mzt). while this process has been well-studied within model species, we have little knowledge of how the pools of maternal and zygotic transcripts evolve. to characterize the evolutionary dynamics and functional constraints ...201830557299
aedes aegypti galectin competes with cry11aa for binding to alp1 to modulate cry toxicity.the key step for the toxicity of bacillus thuringiensis subsp. israelensis (bti) is the interaction between toxins and putative receptors; thus, many studies focus on identification of new toxin receptors and engineering of toxins with higher affinity/specificity for receptors. in the larvae of aedes aegypti, galectin-14 was one of the genes upregulated by bti treatment. rnai knockdown expression of galectin-14 and feeding recombinant galectin-14-thioredoxin fusion protein significantly affected ...201830556692
evaluation of the leaf essential oil from artemisia vulgaris and its larvicidal and repellent activity against dengue fever vector aedes aegypti-an experimental and molecular docking investigation.aedes aegypti is a mosquito vector that spreads dengue fever and yellow fever worldwide in tropical and subtropical countries. essential oil isolated from artemisia vulgaris is found to have larvicidal and repellent action against this vector. the dried leaves were subjected to hydrodistillation using a clevenger-type apparatus for 4 h. the isolated essential oil was analyzed by using gas chromatography-mass spectrometry, and the major insecticidal compounds were identified as α-humulene (0.72%) ...201830556010
omics approaches to study juvenile hormone synthesis.the juvenile hormones (jhs) are a family of insect acyclic sesquiterpenoids produced by the corpora allata (ca), a pair of endocrine glands connected to the brain. they are involved in the regulation of development, reproduction, behavior, caste determination, diapause, stress response, and numerous polyphenisms. in the post-genomics era, comprehensive analyses using functional 'omics' technologies such as transcriptomics, proteomics and metabolomics have increased our understanding of the activ ...201830551825
genetic variability of the aedes aegypti (diptera: culicidae) mosquito in el salvador, vector of dengue, yellow fever, chikungunya and zika.aedes aegypti is associated with dengue, yellow fever, chikungunya and zika viruses. this vector is widespread in tropical and subtropical areas, and can also occur in temperate areas at higher latitudes. the geographical distribution of ae. aegypti continues to spread due to human activities. this is the first study to examine the population genetic structure of this insect in el salvador, central america.201830547835
susceptible and mcry3a resistant corn rootworm larvae killed by a non-hemolytic bacillus thuringiensis cyt1aa mutant.the western corn rootworm (wcr) diabrotica virgifera virgifera causes substantial damage in corn. genetically modified (gm) plants expressing some bacillus thuringiensis (bt) insecticidal cry proteins efficiently controlled this pest. however, changes in wcr susceptibility to these bt traits have evolved and identification of insecticidal proteins with different modes of action against wcr is necessary. we show here for the first time that cyt1aa from bt exhibits toxicity against wcr besides to ...201830546034
aspergillus terreus (trichocomaceae): a natural, eco-friendly mycoinsecticide for control of malaria, filariasis, dengue vectors and its toxicity assessment against an aquatic model organism artemia nauplii.vector-borne diseases like malaria, filariasis, and dengue are transmitted by mosquitoes and they cause global mortality and morbidity due to an increased resistance against commercial insecticides. the present study was aimed to evaluate the neurobehavioral toxicity, knock-down effect, histopathology, ovicidal, adulticidal, and smoke toxicity effect of aspergillus terreus extract against three mosquito species, namely anopheles stephensi, culex quinquefasciatus, and aedes aegypti (diptera: culi ...201830534070
multispecies transcriptomics data set of brugia malayi, its wolbachia endosymbiont wbm, and aedes aegypti across the b. malayi life cycle.here, we present a comprehensive transcriptomics data set of brugia malayi, its wolbachia endosymbiont wbm, and its vector host. this study samples from 16 stages across the entire b. malayi life cycle, including stage 1 through 4 larvae, adult males and females, embryos, immature microfilariae, and mature microfilariae.201830533772
comparison of landmark- and outline-based geometric morphometrics for discriminating mosquito vectors in ratchaburi province, thailand.it is often challenging to identify mosquito vectors in the field based on morphological features due to their similar morphologies and difficulties in obtaining undamaged samples but is required for their successful control. geometric morphometrics (gm) overcomes this issue by analyzing a suite of traits simultaneously and has the added advantages of being easy to use, low cost, and quick. therefore, this research compared the efficiency and precision of landmark- and outline-based gm technique ...201830533435
dengue outbreaks in hawai'i after wwii - a review of public health response and scientific literature.the four serotypes of dengue virus (denv) cause the most important and common arthropod-borne viral diseases in humans. there have been three major dengue outbreaks in hawai'i since 1946. the most recent and largest outbreak occurred on hawai'i island in 2015-2016. this article reviews the public health response to dengue outbreaks over the period 2001-2016, as well as scientific literature on dengue outbreaks in hawai'i. as summarized in the assessment by the centers for disease control and pre ...201830533283
correction: mosquito pornoscopy: observation and interruption of aedes aegypti copulation to determine female polyandric event and mixed progeny.[this corrects the article doi: 10.1371/journal.pone.0193164.].201830532207
vector competence of biting midges and mosquitoes for shuni virus.shuni virus (shuv) is an orthobunyavirus that belongs to the simbu serogroup. shuv was isolated from diverse species of domesticated animals and wildlife, and is associated with neurological disease, abortions, and congenital malformations. recently, shuv caused outbreaks among ruminants in israel, representing the first incursions outside the african continent. the isolation of shuv from a febrile child in nigeria and seroprevalence among veterinarians in south africa suggests that the virus ma ...201830532189
zika in puerto rico, 2016-2017: ii perspectives on epidemic surveillance and control, health communication, outcomes and lessons.the social reaction to the zika epidemic in puerto rico reached a confrontational climax regarding aerial fumigation with an organophosphate insecticide. the public drama has obscured multiple simultaneous controversies. this and a companion paper, based mostly on print and digital news reports, provide a context and description of the major controversies and examine the outcomes and their lessons for the protection of the public's health. part ii covers the questions on disease surveillance (wh ...201830576574
zika virus in the americas: an environmental health perspective.phylogenetic studies suggest that zikv may have been introduced to brazil, and therefore to the americas, in 2014 during the world spring canoe championship held in the city of rio de janeiro. since then the virus has spread across latin america, caribbean, and north america. it seems clear that aedes aegypti and, to a lesser extent, aedes albopictus are the main vectors of the pathogen. zikv infection symptoms are similar to other flaviviruses such as a dengue infection and therefore can be eas ...201830576572
the pagodas protocol: pediatric assessment group of dengue and aedes saliva protocol to investigate vector-borne determinants of aedes-transmitted arboviral infections in cambodia.mosquito-borne arboviruses, like dengue virus, continue to cause significant global morbidity and mortality, particularly in southeast asia. when the infectious mosquitoes probe into human skin for a blood meal, they deposit saliva containing a myriad of pharmacologically active compounds, some of which alter the immune response and influence host receptivity to infection, and consequently, the establishment of the virus. previous reports have highlighted the complexity of mosquito vector-derive ...201830572920
construction sites in miami-dade county, florida are highly favorable environments for vector mosquitoes.urbanization is increasing globally, and construction sites are an integral part of the urbanization process. it is unknown to what extent construction sites create favorable breeding conditions for mosquitoes. the main objectives of the present study were to identify what species of mosquitoes are present at construction sites and the respective physical features associated with their production. eleven construction sites were cross-sectionally surveyed for the presence of mosquitoes in miami-d ...201830571764
mapping the global potential distributions of two arboviral vectors aedes aegypti and ae. albopictus under changing climate.aedes aegypti and ae. albopictus are the primary vectors that transmit several arboviral diseases, including dengue, chikungunya, and zika. the world is presently experiencing a series of outbreaks of these diseases, so, we still require to better understand the current distributions and possible future shifts of their vectors for successful surveillance and control programs. few studies assessed the influences of climate change on the spatial distributional patterns and abundance of these impor ...201830596764
risk prediction system for dengue transmission based on high resolution weather data.dengue is the fastest spreading vector-borne viral disease, resulting in an estimated 390 million infections annually. precise prediction of many attributes related to dengue is still a challenge due to the complex dynamics of the disease. important attributes to predict include: the risk of and risk factors for an infection; infection severity; and the timing and magnitude of outbreaks. in this work, we build a model for predicting the risk of dengue transmission using high-resolution weather d ...201830521550
the composition of midgut bacteria in aedes aegypti (diptera: culicidae) that are naturally susceptible or refractory to dengue viruses.the composition, abundance, and diversity of midgut bacteria in mosquitoes can influence pathogen transmission. we used 16s rrna microbiome profiling to survey midgut microbial diversity in pooled samples of laboratory colonized dengue-refractory, cali-mib, and dengue-susceptible, cali-s aedes aegypti (linnaeus). the 16s rrna sequences from the sugar-fed midguts of adult females clustered to 63 amplicon sequence variants (asvs), primarily from proteobacteria, firmicutes, flavobacteria, and actin ...201830508201
optimization of double-stranded rnai intrathoracic injection method in aedes aegypti.rna interference is widely used to analyze gene functions via phenotypic knockdown of target transcripts in mosquitoes, which transmit numerous mosquito-borne diseases. functional analysis of mosquito genes is indispensable to understand and reduce transmission of mosquito-borne diseases in mosquitoes. intrathoracic injection of double-stranded rna (dsrna) remains the simplest and most customizable method in mosquitoes for functional analysis of the genes of interest. however, achieving consiste ...201830505333
[costs of an aedes aegypti vector control program in municipalities in colombia: a case study in girón and guadalajara de buga, 2016].the aim of this study was to measure the costs of vector-borne disease control programs at the local level in colombia (2016). a cost analysis was performed for this purpose from the policymaker's perspective in the municipalities (counties) of girón and guadalajara de buga, specifically for the aedes aegypti control program, the principal mosquito vector of dengue, zika, and chikungunya. the analysis involved the quantification of all the costs required for each of the prevention and control st ...201830517314
sequential infection of aedes aegypti mosquitoes with chikungunya virus and zika virus enhances early zika virus transmission.in urban settings, chikungunya, zika, and dengue viruses are transmitted by aedes aegypti mosquitoes. since these viruses co-circulate in several regions, coinfection in humans and vectors may occur, and human coinfections have been frequently reported. yet, little is known about the molecular aspects of virus interactions within hosts and how they contribute to arbovirus transmission dynamics. we have previously shown that aedes aegypti exposed to chikungunya and zika viruses in the same blood ...201830513725
alternative insecticides for larval control of the dengue vector aedes aegypti in lao pdr: insecticide resistance and semi-field trial study.the mosquito aedes aegypti is the primary vector of several arboviruses, such as dengue, chikungunya and zika, and represents a major public health problem in southeast asia. in laos, where dengue is reemerging, several ae. aegypti populations from the capital vientiane have shown resistance to the organophosphate temephos, a commonly-used larvicide for public health interventions.201830509299
a scoping review of published literature on chikungunya virus.chikungunya virus (chikv) has caused several major epidemics globally over the last two decades and is quickly expanding into new areas. although this mosquito-borne disease is self-limiting and is not associated with high mortality, it can lead to severe, chronic and disabling arthritis, thereby posing a heavy burden to healthcare systems. the two main vectors for chikv are aedes aegypti and aedes albopictus (asian tiger mosquito); however, many other mosquito species have been described as com ...201830496207
mosquitoes modulate leg dynamics at takeoff to accommodate surface roughness.insects perform takeoffs from a nearly unquantifiable number of surface permutations and many use their legs to initiate upward movement prior to the onset of wingbeats, including the mosquito. in this study we examine the unprovoked pre-takeoff mechanics of aedes aegypti mosquitoes from two surfaces of contrasting roughness, one with roughness similar to polished glass and the other comparable to the human forearm. using high-speed videography, we find mosquitos exhibit two distinct leg actions ...201830479315
a novel highly divergent strain of cell fusing agent virus (cfav) in mosquitoes from the brazilian amazon region.classical insect-specific flaviviruses (cisfs) have been widely detected in different countries in the last decades. here, we characterize the near full-length genomes of two cisfs detected in mosquitoes collected in the city of macapá, state of amapá, amazon region of brazil. a total of 105 pools of female mosquitos were analyzed by next-generation sequencing (ngs). comparative genomics and phylogenetic analysis identified three strains of cell fusing agent virus (cfav) and two of culex flavivi ...201830477235
void entry by aedes aegypti (diptera: culicidae) mosquitoes is lower than would be expected by a randomized search.insects enter every passible space on the planet. despite our best efforts, flying insects infiltrate slightly open windows in domiciles, automobiles, storage spaces, and more. is this ubiquitous experience a consequence of insect abundance and probability, or are flying insects adept at detecting passageways? there remains a lack of understanding of insect effectiveness in finding passage through the voids and imperfections in physical barriers in response to attractants, a topic particularly c ...201830476211
global spatial assessment of aedes aegypti and culex quinquefasciatus: a scenario of zika virus exposure.zika virus (zikv) is an arbovirus transmitted mainly by aedes aegypti mosquitoes. recent scientific evidence on culex quinquefasciatus has suggested its potential as a vector for zikv, which may change the current risk zones. we aimed to quantify the world population potentially exposed to zikv in a spatially explicit way, considering the primary vector (a. aegypti) and the potential vector (c. quinquefasciatus). our model combined species distribution modelling of mosquito species with spatiall ...201830474578
recent history of aedes aegypti: vector genomics and epidemiology records.aedes aegypti bears the common name "the yellow fever mosquito," although, today, it is of more concern as the major vector of dengue, chikungunya, and, most recently, zika viruses. in the present article, we review recent work on the population genetics of this mosquito in efforts to reconstruct its recent (approximately 600 years) history and relate these findings to epidemiological records of occurrences of diseases transmitted by this species. the two sources of information are remarkably co ...201830464351
longevity of mass-reared, irradiated and packed male anopheles arabiensis and aedes aegypti under simulated environmental field conditions.to ensure the success of a mosquito control programme that integrates the sterile insect technique (sit), it is highly desirable to release sterile males with a maximal lifespan to increase release effectiveness. understanding sterile male survival under field conditions is thus critical for determining the number of males to be released. our study aimed to investigate the effect of mass rearing, irradiation, chilling, packing and release time on irradiated male mosquito longevity.201830463624
vertical transmission of naturally occurring bunyamwera and insect-specific flavivirus infections in mosquitoes from islands and mainland shores of lakes victoria and baringo in kenya.many arboviruses transmitted by mosquitoes have been implicated as causative agents of both human and animal illnesses in east africa. although epidemics of arboviral emerging infectious diseases have risen in frequency in recent years, the extent to which mosquitoes maintain pathogens in circulation during inter-epidemic periods is still poorly understood. this study aimed to investigate whether arboviruses may be maintained by vertical transmission via immature life stages of different mosquit ...201830452443
cyp-mediated permethrin resistance in aedes aegypti and evidence for trans-regulation.aedes aegypti poses a serious risk to human health due to its wide global distribution, high vector competence for several arboviruses, frequent human biting, and ability to thrive in urban environments. pyrethroid insecticides remain the primary means of controlling adult a. aegypti populations during disease outbreaks. as a result of decades of use, pyrethroid resistance is a global problem. cytochrome p450 monooxygenase (cyp)-mediated detoxification is one of the primary mechanisms of pyrethr ...201830452436
air versus water temperature of aquatic habitats in delhi: implications for transmission dynamics of aedes aegypti.the present study was planned to characterize the microclimate experienced by aedes larvae in different breeding habitats by determining the temperature variations in water kept in containers during different months under natural conditions. the study was conducted in three municipal zones of delhi. in each site, four types of container material (plastic, cement, iron and ceramic) were chosen for recording the water temperature in the containers. daily air and water temperatures (mean, maximum a ...201830451475
operational satellite-based temporal modelling of aedes population in argentina.aedes aegypti is a vector for chikungunya, dengue and zika viruses in latin america and is therefore a large public health problem for the region. for this reason, several inter-institutional and multidisciplinary efforts have been made to support vector control actions through the use of geospatial technologies. this study presents the development of an operational system for the application of free access to remotely sensed products capable of assessing the oviposition activity of ae. aegypti ...201830451465
innate immune response of primary human keratinocytes to west nile virus infection and its modulation by mosquito saliva.west nile virus (wnv) is a flavivirus involved in many human infections worldwide. this arthropod-borne virus is directly co-inoculated with mosquito saliva through the epidermis and the dermis during blood meal. wnv starts replicating in the skin before migrating to the draining lymph node, leading to widespread viremia and in some cases to neurological symptoms. skin is a complex organ composed of different cell types that together perform essential functions such as pathogen sensing, barrier ...201830450338
pharmacological inhibition of inward rectifier potassium channels induces lethality in larval aedes aegypti.the inward rectifier potassium (kir) channels play key roles in the physiology of mosquitoes and other insects. our group, among others, previously demonstrated that small molecule inhibitors of kir channels are promising lead molecules for developing new insecticides to control adult female mosquitoes. however, the potential use of kir channel inhibitors as larvicidal agents is unknown. here we tested the hypothesis that pharmacological inhibition of kir channels in the larvae of aedes aegypti, ...201830445675
a comprehensive review of the manifestations and pathogenesis of zika virus in neonates and adults.the zika virus (zikv) has been slowly becoming an epidemic in different parts of the world. since its discovery in 1947, there have been numerous outbreaks affecting many different populations. currently, there is an ongoing threat of zikv in latin america and the united states of america. zikv is mainly spread by the aedesaegypti mosquito and causes non-specific symptoms such as fever, myalgia, and generalized weakness. in addition to these symptoms, it has been noted the zikv is capable of cau ...201830443460
competition among aedes aegypti larvae.adult aedes aegypti mosquitoes are important vectors of human disease. the size of the adult female affects her success, fitness, and ability to transmit diseases. the size of the adults is determined during the aquatic larval stage. competition among larvae for food influences the size of the pupa and thus the adult. in these experiments, the food level (mg/larva) and the density (larvae/vial) both affect intraspecific competition, which shows up as the interaction of the two factors. furthermo ...201830439951
improved reference genome of aedes aegypti informs arbovirus vector control.female aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, zika and chikungunya. progress in understanding the biology of mosquitoes and developing the tools to fight them has been slowed by the lack of a high-quality genome assembly. here we combine diverse technologies to produce the markedly improved, fully re-annotated aaegl5 genome assembly, and demonstrate how it accelerates mosquito science. we anchored phys ...201830429615
restriction of zika virus infection and transmission in aedes aegypti mediated by an insect-specific flavivirus.previous studies demonstrated an insect-specific flavivirus, nhumirim virus (nhuv), can suppress growth of west nile virus (wnv) and decrease transmission rates in nhuv/wnv co-inoculated culex quinquefasciatus. to assess whether nhuv might interfere with transmission of other medically important flaviviruses, the ability of nhuv to suppress viral growth of zika virus (zikv) and dengue-2 virus (denv-2) was assessed in aedes albopictus cells. significant reductions in zikv (100,000-fold) and denv- ...201830429457
conserved motifs in the hypervariable domain of chikungunya virus nsp3 required for transmission by aedes aegypti mosquitoes.chikungunya virus (chikv) is a re-emerging arthropod-borne (arbo)virus that causes chikungunya fever in humans and is predominantly transmitted by aedes aegypti mosquitoes. the chikv replication machinery consists of four non-structural proteins (nsp1-4) that additionally require the presence of a number of host proteins for replication of the viral rna. nsp3 is essential for chikv replication and has a conserved macro, central and c-terminal hypervariable domain (hvd). the hvd is intrinsically ...201830412583
behavioral responses of gravid culex quinquefasciatus, aedes aegypti, and anopheles quadrimaculatus mosquitoes to aquatic macrophyte volatiles.mosquitoes use many cues to assess whether a habitat is conducive for reproduction, possibly including the presence of stimuli from aquatic macrophytes. the effect of water infusions of water hyacinth (eichhornia crassipes), water lettuce (pista stratioles), parrotfeather (myriophyllum aquaticum), and water pennywort (hydrocotyle umbellata) on mosquito oviposition and attraction was investigated. gravid culex quinquefasciatus deposited significantly more egg rafts in water hyacinth, water lettuc ...201830408300
effects of the environmental temperature on aedes aegypti and aedes albopictus mosquitoes: a review.the temperature of the environment is one of the most important abiotic factors affecting the life of insects. as poikilotherms, their body temperature is not constant, and they rely on various strategies to minimize the risk of thermal stress. they have been thus able to colonize a large spectrum of habitats. mosquitoes, such as ae. aegypti and ae. albopictus, vector many pathogens, including dengue, chikungunya, and zika viruses. the spread of these diseases has become a major global health co ...201830404142
anopheles mosquitoes may drive invasion and transmission of mayaro virus across geographically diverse regions.the togavirus (alphavirus) mayaro virus (mayv) was initially described in 1954 from mayaro county (trinidad) and has been responsible for outbreaks in south america and the caribbean. imported mayv cases are on the rise, leading to invasion concerns similar to chikungunya and zika viruses. little is known about the range of mosquito species that are competent mayv vectors. we tested vector competence of 2 mayv genotypes in laboratory strains of six mosquito species (aedes aegypti, anopheles free ...201830403665
insecticide resistance and fitness: the case of four aedes aegypti populations from different brazilian regions.chemical control is still a major strategy to constrain vector density and mitigate pathogen transmission. however, insecticide overuse poses a high selective pressure, favouring the spread of resistance alleles in natural populations. in an insecticide-free environment, a fitness cost is expected in resistant insects when compared to susceptible counterparts. this study investigates whether insecticide resistance to an organophosphate (temephos) and a pyrethroid (deltamethrin) is associated wit ...201830402487
inspiratory muscle training with isokinetic device to help ventilatory weaning in a patient with guillain-barré syndrome by zika virus.zika is an arbovirus infection mainly transmitted by the mosquito aedes aegypti. in 2016, the burden of zika epidemic in brazil was significant. patients affected by zika virus can develop guillain-barré syndrome, evolving to muscle respiratory failure requiring mechanical ventilation. in this setting, delayed recovery of the muscle weakness can result in prolonged weaning, a condition that by itself is related to a high mortality rate. the study is reporting a case of a patient with zika and gu ...201830402297
chemical composition and larvicidal activities of azolla pinnata extracts against aedes (diptera:culicidae).the resistance problem of dengue vectors to different classes of insecticides that are used for public health has raised concerns about vector control programmes. hence, the discovery of alternative compounds that would enhance existing tools is important for overcoming the resistance problem of using insecticides in vectors and ensuring a chemical-free environment. the larvicidal effects of azolla pinnata extracts by using two different extraction methods with methanol solvent against aedes in ...201830399167
yellow fever outbreak in brazil: the puzzle of rapid viral spread and challenges for immunisation.we discuss the complex eco-social factors involved in the puzzle of the unexpected rapid viral spread in the ongoing brazilian yellow fever (yf) outbreak, which has increased the reurbanisation risk of a disease without urban cases in brazil since 1942. indeed, this rapid spatial viral dissemination to the southeast and south regions, now circulating in the atlantic forest fragments close to peri-urban areas of the main brazilian megalopolises (são paulo and rio de janeiro) has led to an exponen ...201830427974
courtship activity, copulation & insemination success in a mosquito vector fed a herbal aphrodisiac: implications for sterile insect technology.in sterile insect technology (sit), mating competitiveness is a pre-condition for the reduction of target pest populations and a crucial parameter for judging efficacy. still, current sit trials are being hindered by decreased effectiveness due to reduced sexual performance of released males. here, we explored the possible role of a herbal aphrodisiac in boosting the mating activity of aedes aegypti.201830425225
the spread of mosquito-borne viruses in modern times: a spatio-temporal analysis of dengue and chikungunya.since the 1970s, mosquito-borne pathogens have spread to previously disease-free areas, as well as causing increased illness in endemic areas. in particular, dengue and chikungunya viruses, transmitted primarily by aedes aegypti and secondarily by aedes albopictus mosquitoes, represent a threat for up to a third of the world population, and are a growing public health concern. in this study, we assess the spatial and temporal factors related to the occurrences of historic dengue and chikungunya ...201830390927
improvement of mosquito identification by maldi-tof ms biotyping using protein signatures from two body parts.matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technology (maldi-tof ms) is an innovative tool that has been shown to be effective for the identification of numerous arthropod groups including mosquitoes. a critical step in the implementation of maldi-tof ms identification is the creation of spectra databases (db) for the species of interest. mosquito legs were the body part most frequently used to create identification db. however, legs are one of the most fragile ...201830390691
toxic activity, molecular modeling and docking simulations of bacillus thuringiensis cry11 toxin variants obtained via dna shuffling.the cry11 family belongs to a large group of δ-endotoxins that share three distinct structural domains. among the dipteran-active toxins referred to as three-domain cry11 toxins, the cry11aa protein from bacillus thuringiensis subsp. israelensis (bti) has been the most extensively studied. despite the potential of bti as an effective biological control agent, the understanding of cry11 toxins remains incomplete. in this study, five cry11 variants obtained via dna shuffling displayed toxic activi ...201830386315
toward implementation of mosquito sterile insect technique: the effect of storage conditions on survival of male aedes aegypti mosquitoes (diptera: culicidae) during transport.sterile insect technique (sit) is a promising, environmentally friendly alternative to the use of pesticides for insect pest control. however, implementing sit with aedes aegypti (linnaeus) mosquitoes presents unique challenges. for example, during transport from the rearing facility to the release site and during the actual release in the field, damage to male mosquitoes should be minimized to preserve their reproductive competitiveness. the short flight range of male ae. aegypti requires elabo ...201830383264
isolation and identification of mosquito biting deterrents from the north american mosquito repelling folk remedy plant, matricaria discoidea dc.matricaria discoidea dc. (asteraceae) has documented use as an insect repellent by blackfoot indians and other indigenous groups of north america. this investigation was conducted to evaluate this practice and systematically identify chemical constituents responsible for any insect repelling effect by utilizing a mosquito (aedes aegypti (linnaeus)) biting deterrent bioactivity-directed purification approach. hydrodistilled oil from dried aerial parts of m. discoidea was the most bioactive crude ...201830379955
control of dengue virus in the midgut of aedes aegypti by ectopic expression of the dsrna-binding protein loqs2.dengue virus (denv) is an arbovirus transmitted to humans by aedes mosquitoes1. in the insect vector, the small interfering rna (sirna) pathway is an important antiviral mechanism against denv2-5. however, it remains unclear when and where the sirna pathway acts during the virus cycle. here, we show that the sirna pathway fails to efficiently silence denv in the midgut of aedes aegypti although it is essential to restrict systemic replication. accumulation of denv-derived sirnas in the midgut re ...201830374169
new sulphated flavonoids and larvicidal activity of helicteres velutina k. schum (sterculiaceae).helicteres velutina k. schum (sterculiaceae), commonly known in brazil as 'pitó', is traditionally used by indigenous peoples as insecticides and repellents. the present work reports on the the phytoconstituents from aerial parts of h. velutina and evaluation of the larvicidal potential of its extract. the compounds were isolated using chromatographic techniques and identified by nmr, ir and lc-hrms. this study led to the isolation of a fatty acid, one aliphatic alcohol, four chlorophyll derivat ...201830373225
wing morphometric variability in aedes aegypti (diptera: culicidae) from different urban built environments.aedes aegypti is the main vector of the dengue, zika and several other arboviruses. it is highly adapted to urbanized environments and can be found worldwide. mosquito population control is considered the best strategy for fighting mosquito-borne diseases, making an understanding of their population dynamics vital for the development of more effective vector control programs. this study therefore sought to investigate how different levels of urbanization affect aedes aegypti populations and modu ...201830367678
generation of axenic aedes aegypti demonstrate live bacteria are not required for mosquito development.the mosquito gut microbiome plays an important role in mosquito development and fitness, providing a promising avenue for novel mosquito control strategies. here we present a method for rearing axenic (bacteria free) aedes aegypti mosquitoes, consisting of feeding sterilized larvae on agar plugs containing a high concentration of liver and yeast extract. this approach allows for the complete development to adulthood while maintaining sterility; however, axenic mosquito's exhibit delayed developm ...201830367055
odor-specific daily rhythms in the olfactory sensitivity and behavior of aedes aegypti mosquitoes.many biological processes and behaviors in mosquitoes display rhythmic patterns, allowing for fine tuning to cyclic environmental conditions. in mosquitoes, vector-host interactions are primarily mediated by olfactory signals. previous studies have established that, in the malaria vector anopheles gambiae, rhythmic expression of odorant binding proteins and takeout proteins in the antenna resulted in a corresponding rhythm in olfactory sensitivity to relevant host odors. however, it remained unc ...201830360532
salivary gland extract from aedes aegypti improves survival in murine polymicrobial sepsis through oxidative mechanisms.sepsis is a systemic disease with life-threatening potential and is characterized by a dysregulated immune response from the host to an infection. the organic dysfunction in sepsis is associated with the production of inflammatory cascades and oxidative stress. previous studies showed that aedes aegypti saliva has anti-inflammatory, immunomodulatory, and antioxidant properties. considering inflammation and the role of oxidative stress in sepsis, we investigated the effect of pretreatment with sa ...201830360497
host blood-meal source has a strong impact on gut microbiota of aedes aegypti.gut microbial communities of mosquitoes can influence vector susceptibility to pathogens, yet the factors that govern their composition remain poorly understood. we investigated the impact of host blood-meal source on gut microbiota of aedes aegypti l. adult mosquitoes were fed on human, rabbit or chicken blood and their gut microbiota compared to those of sugar-fed and newly emerged adults. microbial diversity was significantly reduced in blood-fed and sugar-fed mosquitoes but was restored to t ...201930357406
a statewide survey of container aedes mosquitoes (diptera: culicidae) in north carolina, 2016: a multiagency surveillance response to zika using ovitraps.native and invasive container-inhabiting aedes mosquitoes (diptera: culicidae) play important roles in the transmission of endemic and traveler-introduced arboviruses in the united states. in response to the emergence of zika virus into the americas, we surveyed the distribution of container aedes spp. of public health importance within north carolina during 2016 using ovitraps. a seasonal survey was conducted in 18 counties from the mountains to the coast to identify species incriminated in the ...201930380070
isolation and growth inhibition potential of entomopathogenic nematodes against three public health important mosquito vectors.the prevalence of mosquito vector borne diseases and the resistance of mosquitoes to conventional pesticides have been of important public concern to the mosquito endemic countries. present study was conducted to identify the native bio-larvicidal potential of the entomopathogenic nematodes; steinernema siamkayai (kpr-4) heterohabditis indica (kpr-8), steinernema glaseri and steinernema abbasi. the isolated nematodes were subsequently cultured and evaluated their larvicidal potential against the ...201930414843
preparation and use of a yeast shrna delivery system for gene silencing in mosquito larvae.the mosquito genome projects facilitated research in new facets of mosquito biology, including functional genetic studies in the dengue and zika virus vector aedes aegypti and the primary african malaria vector anopheles gambiae. rna interference (rnai) has facilitated gene silencing experiments in both of these disease vector mosquito species and could one day be applied as a new method of vector control. here, we describe a procedure for the genetic engineering of saccharomyces cerevisiae (bak ...201930414120
inhibition of sodium-hydrogen antiport by antibodies to nha1 in brush border membrane vesicles from whole aedes aegypti larvae.the present research report describes na+/h+ antiport by brush border membrane vesicles isolated from whole larvae of aedes aegypti (aebbmvw). our hypothesis is that acid quenching of acridine orange by aebbmvw is predominantly mediated by na+/h+ antiport via the nha1 component of the aebbmvw in the absence of amino acids and atp. aenha1 is a na+/h+ antiporter that has been postulated to exchange na+ and h+ across the apical plasma membrane in posterior midgut of a. aegypti larvae. its principal ...201930392010
the combinatory effect of cyt1aa flexibility and specificity against dipteran larvae improves the toxicity of bacillus thuringensis kurstaki toxins.cyt1a98 is a novel cytolytic protein, from bupm98 bacillus thuringiensis strain, characterized by its synergistic activity with b. thuringiensis kurstaki toxins against lepidopteran larvae. in this study, we evidenced that cyt1a98 improves the toxicity of b. thuringiensis kurstaki toxins against aedes aegypti larvae. in fact, the strain bns3phtcyt1a98 exhibited a larvicidal activity of about 849-fold of that of bns3phtblue against a. aegypti. the molecular and biochemical characterizations, of c ...201930391590
bioassay and molecular study for detection of insecticide resistance dengue causing mosquito vectors.nowadays, dengue infection creates a major problem across the country. the vector species carrying dengue infection has progressively started to developed resistance against most of the currently used insecticides. hence, a study was carried out in dengue-endemic areas of assam and arunachal pradesh to find the current situation of insecticide susceptibility status of dengue vectors. based on the previous history of dengue incidence, aedes mosquitoes were collected from dibrugarh, kamrup, sivasa ...201930429402
autocidal gravid ovitrap incorporation with attractants for control of gravid and host-seeking aedes aegypti (diptera: culicidae).aedes aegypti linnaeus (diptera: culicidae) is a primary vector of multiple arboviruses including zika, dengue, chikungunya, and yellow fever. after incorporating bg lure or bg lure + octenol, the cdc's autocidal gravid ovitrap (ago) was more effective at collecting and controlling host-seeking female ae. aegypti than gravid female mosquitoes. the addition of octenol to the ago did not increase the number of female mosquitoes captured by the ago, compared with the ago alone. the ago baited with ...201930462293
monitoring insecticide resistance profiles of aedes aegypti (diptera: culicidae) in the sunda islands of indonesia based on diagnostic doses of larvicides.this study was conducted to monitor the susceptibility status of aedes aegypti (linnaeus) larvae in the sunda islands of indonesia against various organophosphates and organochlorines. larval bioassay was performed in accordance with the world health organization standard protocol. field-collected and reference strains of ae. aegypti larvae were tested against diagnostic doses of eight larvicides belonging to organophosphates and organochlorines, namely bromophos (0.050 mg/liter), chlopyrifos (0 ...201930462258
past, present and future of aedes aegypti in its south american southern distribution fringe: what do temperature and population tell us?aedes aegypti (diptera: culicidae) (linnaeus) is currently the major threat among arbovirus vectors in the americas. we examined its past, present, and future distribution patterns in the south american fringe in association with environmental and demographic variables at two spatial scales. we updated the database of the occurrence of ae. aegypti per locality and modelled by glmm the past occurrence (until 2000) and its expansion (2001-2017) as a function of air temperature, precipitation, alti ...201930458122
water channel activity of putative aquaporin-6 present in aedes aegypti.aquaporins (aqps) are integral membrane channels that facilitate the bidirectional transport of water and sometimes other small solutes across biological membranes. aqps are important in mediating environmental adaptations in mosquitoes and are considered as a novel target for the development of effective insecticides against mosquitoes. here, we expressed aedes aegypti aqp6 ( aaaqp6) in human embryonic kidney (hek) 293 cells and analyzed the water permeability by a conventional swelling assay, ...201930456765
saccharomyces cerevisiae (baker's yeast) as an interfering rna expression and delivery system.the broad application of rna interference for disease prevention is dependent upon the production of dsrna in an economically feasible, scalable, and sustainable fashion, as well as the identification of safe and effective methods for rna delivery. current research has sparked interest in the use of saccharomyces cerevisiae for these applications. this review examines the potential for commercial development of yeast interfering rna expression and delivery systems. s. cerevisiae is a genetic mod ...201930474529
ecotoxicity response of chlorpyrifos in aedes aegypti larvae and lactuca sativa seeds after uv/h2o2 and uvc oxidation.chlorpyrifos (cp) is an organophosphate pesticide widely used in agriculture known to cause neurological and immunological effects in addition to interfering in the reproduction and development of organisms. in this study, cp degradation by uv/h2o2 process and uvc radiation was investigated, and the ecotoxicity and phytotoxicity was evaluated using bioassays of aedes aegypti larvae and lactuca sativa seeds. cp degradation was monitored by hplc-dad, and kinetic parameters were calculated for all ...201930471582
coinfection of chikungunya and dengue viruses: a serological study from north western region of punjab, india.dengue and chikungunya (chik) infections appear to be increasing in all parts of india. aedes aegypti mosquitoes are common vectors for dengue virus (denv) and chik virus (chikv). in areas where both viruses cocirculate, they can be transmitted together. there are very few studies discussing the dengue-chik coinfection from punjab region of india. the present study was undertaken to study the clinical features of dengue-chik coinfection and compare with monoinfection.201930498319
volatile composition and biological activities of the leaf essential oil from zanthoxylum limoncello grown in oaxaca, méxico.zanthoxylum limoncello is a native plant from southern mexico which is used as a timber source, condiment and as a traditional medicine. herein, we report on the volatile content of the leaf essential oil and its biological activities. the annual essential oils (2015-2018) contained volatile organic compounds which exhibited a moderate growth inhibitory activity against h. pylori atcc 53504 (mic 121.4-139.7 μg ml-1 ), 26695 (mic 85.5-94.9 μg ml-1 ) and j99 (mic 94.7-110.4 μg ml-1 ). these hydrod ...201930512233
differential suppression of persistent insect specific viruses in trans-infected wmel and wmelpop-cla aedes-derived mosquito lines.wolbachia suppresses the replication of +ssrna viruses such as dengue and zika viruses in aedes aegypti mosquitoes. however, the range of viruses affected by this endosymbiont is yet to be explored. recently, novel insect-specific viruses (isvs) have been described from numerous mosquito species and mosquito-derived cell lines. cell-fusing agent virus (flaviviridae) and phasi charoen-like virus (bunyaviridae) persistently infect the ae. aegypti cell line aag2 which has been used for experimental ...201930503908
Refresh
firstprev
...141142143144145146147148149150
nextlast
Displaying items 14201 - 14300 of 14974