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breaking down polystyrene through the application of a two-step thermal degradation and bacterial method to produce usable byproducts.in this work, the objective is to examine a novel approach on the biodegradability of polystyrene is examined. a two-step method for the degradation of polystyrene to chemically useful products has been devised. initially, polystyrene is liquefied via a thermal degradation procedure predominantly into styrene monomers at low temperatures and left to cool down. application of microorganisms in a second step targets the breaking of the polymer into smaller organic molecules. microorganisms tested ...201727440221
the effect of berberine hydrochloride on enterococcus faecalis biofilm formation and dispersion in vitro.enterococcus faecalis (e. faecalis) is one of the major causes of biofilm infections. berberine hydrochloride (bbh) has diverse pharmacological effects; however, the effects and mechanisms of bbh on e. faecalis biofilm formation and dispersion have not been reported. in this study, 99 clinical isolates from the urine samples of patients with urinary tract infections (utis) were collected and identified. ten strains of e. faecalis with biofilm formation ability were studied. bbh inhibited e. faec ...201727242142
pretreatment with a heat-killed probiotic modulates monocyte chemoattractant protein-1 and reduces the pathogenicity of influenza and enterovirus 71 infections.it has been proposed that inactivated probiotics may modulate the host immune system and contribute to mitigation of viral infections. this study demonstrated that administration of heat-killed enterococcus faecalis, a widely used probiotic, can protect host animals against viral infections. the influenza-mediated morbidity and lung inflammation in e. faecalis-treated mice decreased significantly compared with those of the control mice. furthermore, we found that the protection is associated wit ...201727072606
rapid assessment of resistance to antibiotic inhibitors of protein synthesis in the gram-positive pathogens, enterococcus faecalis and streptococcus pneumoniae, based on evaluation of the lytic response.a novel assay for rapid determination of resistance to antibiotic inhibitors of protein synthesis was developed for the gram-positive pathogens, enterococcus faecalis and streptococcus pneumoniae. to this purpose, a lytic response was obtained by a brief incubation with lysozyme or a mixture of lysozyme, triton x-100, and edta for e. faecalis (n = 82) and s. pneumoniae (n = 51), respectively. lysis was quantified by visualizing the released nucleoids. antibiotic-susceptible bacteria treated with ...201727383851
detection of vancomycin resistance in enterococcus species isolated from clinical samples and feces of colonized patients by phenotypic and genotypic methods.the aim of this study was to find out the clinical correlation between the presence of vancomycin-resistant genes (van a and van b) and their expression as detected by phenotypic tests in colonized patients and in clinical isolates.201727166038
efficacy of calcium oxide and calcium hydroxide nanoparticles on the elimination of enterococcus faecalis in human root dentin.the objective of this study was to compare the antibacterial effect of calcium oxide nanoparticles (conps) and calcium hydroxide nanoparticles (chnps) against enterococcus faecalis in a dentinal block model.201727129586
light and temperature effects on bioactivity in diatoms.isolates of five pelagic north atlantic marine diatoms (bacillariophyceae): attheya longicornis, chaetoceros socialis, chaetoceros furcellatus, skeletonema marinoi and porosira glacialis were cultivated in large photobioreactors at two light and two temperature regimes to test if this affected bioactivity. we screened for bioactivity in assays representing five different therapeutic areas: diabetes ii (ptp1b), cancer (melanoma cells, a2058), anti-oxidants (frap), immunomodulation (tnfa) and anti ...201727057087
influence of temperature on the antibacterial activity of sodium hypochlorite.the aim of this study was to compare the antimicrobial activity of 5.25% naocl, hypoclean and chlor-xtra at 20 °c and 45 °c in bovine root dentin. one-hundred-and-seventy dentin tubes prepared from bovine maxillary incisors were infected for 21 days with enterococcus faecalis. the specimens were divided into the following groups: 1. 5.25% naocl 20 °c; 2. hypoclean 20 °c; 3. chlor-xtra 20 °c; 4. 5.25% % naocl 45 °c; 5. hypoclean 45 °c; 6. chlor-xtra 45 °c; 7. positive control; 8. negative control ...201727007342
2-hydroxyisocaproic acid is bactericidal in human dental root canals ex vivo.to compare the activity of 2-hydroxyisocaproic acid (hica), calcium hydroxide (ca(oh)2 ) and chlorhexidine digluconate (chg) against enterococcus faecalis t-75359 (root canal isolate) in the root canals of extracted human teeth.201727004733
effect of laser-activated irrigation on biofilms in artificial root canals.to evaluate the antimicrobial effect of laser-activated irrigation (lai) on biofilms formed in simulated root canals.201727014832
molecular characterization of virulence genes in vancomycin-resistant and vancomycin-sensitive enterococci.the aim of this study was to find out the correlation between presence of virulence (gelatinase [gel e], enterococcal surface protein [esp], cytolysin a [cyl a], hyaluronidase [hyl], and aggregation substance [asa1]) and vancomycin-resistant genes (van a and van b) in enterococci, with their phenotypic expression.201727013840
community acquired enterococcal urinary tract infections and antibiotic resistance profile in north india.urinary tract infections (utis) remain a major problem both in hospitalized and outdoor patients. multidrug-resistant enterococci are emerging as a major nosocomial pathogen with increasing frequency. however, the incidence of community-acquired enterococcal infections and species prevalent in india is not thoroughly investigated.201727013814
enhancing the antibacterial activity of the gold standard intracanal medicament with incorporation of silver zeolite: an in vitro study.enterococcus faecalis is a persistent organism that plays a major role in the etiology of persistent periradicular lesions after root canal treatment has been associated with different forms of periradicular disease including primary endodontic infections and persistent infections. the present study compares the antibacterial activities of calcium hydroxide, calcium hydroxide mixed with silver zeolite, and calcium hydroxide mixed with 2% chlorhexidine against e. faecalis using direct contact tes ...201727011937
antimicrobial effect of three disinfecting agents on resilon cones and their effect on surface topography: an in vitro study.the objective of this study was to evaluate the effectiveness of 5.25% sodium hypochlorite (naocl), 2% chlorhexidine (chx), and 2% peracetic acid (paa) in disinfecting resilon cones and to evaluate topographical changes microscopically under scanning electron microscope (sem) after rapid chemical disinfection.201727099418
effectiveness of n-acetyl cysteine, 2% chlorhexidine, and their combination as intracanal medicaments on enterococcus faecalis biofilm.the purpose of this study was to evaluate the antibacterial efficacies of 2% chlorhexidine (chx), n-acetyl cysteine (nac) and assess their synergistic or antagonist action as intracanal medicament.201726957787
comparative antimicrobial efficacy of herbal alternatives (emblica officinalis, psidium guajava), mtad, and 2.5% sodium hypochlorite against enterococcus faecalis: an in vitro study.the objective of this study was to evaluate the antimicrobial efficacy of herbal alternatives (emblica officinalis, psidium guajava), biopure mtad, and 2.5% sodium hypochlorite against enterococcus faecalis.201726937369
in vitro antimicrobial evaluation of toothpastes with natural compounds.this in vitro study evaluated the antimicrobial effects of commercial toothpastes containing natural compounds.201726929699
a new mechanism of post-transfer editing by aminoacyl-trna synthetases: catalysis of hydrolytic reaction by bacterial-type prolyl-trna synthetase.aminoacyl trna synthetases are enzymes that specifically attach amino acids to cognate trnas for use in the ribosomal stage of translation. for many aminoacyl trna synthetases, the required level of amino acid specificity is achieved either by specific hydrolysis of misactivated aminoacyl-adenylate intermediate (pre-transfer editing) or by hydrolysis of the mischarged aminoacyl-trna (post-transfer editing). to investigate the mechanism of post-transfer editing of alanine by prolyl-trna synthetas ...201726886480
antimicrobial effect of an oxazolidinone, lantibiotic and calcium hydroxide against enterococcus faecalis biofilm: an in vitro study.the aim was to evaluate and compare the antimicrobial efficacy of an oxazolidinone (linezolid [lz]), lantibiotic (nisin), and calcium hydroxide against enterococcus faecalis biofilm formed on tooth substrate after 2 and 7 days.201726752879
antimicrobial efficacy of different root canal sealers by using real-time polymerase chain reaction: an ex vivo study.root canal sealers help to minimize leakage, provides antimicrobial activity to reduce the possibility of residual bacteria, and to resolve periapical lesion.201726752843
lactobacillus helveticus lafti l10 supplementation modulates mucosal and humoral immunity in elite athletes: a randomized, double-blind, placebo-controlled trial.michalickova, dm, kostic-vucicevic, mm, vukasinovic-vesic, md, stojmenovic, tb, dikic, nv, andjelkovic, ms, djordjevic, bi, tanaskovic, bp, and minic, rd. lactobacillus helveticus lafti l10 supplementation modulates mucosal and humoral immunity in elite athletes: a randomized, double-blind, placebo-controlled trial. j strength cond res 31(1): 62-70, 2017-to test the influence of probiotic supplementation on humoral immune response, a double-blind, placebo-controlled trial was conducted. thirty a ...201727100317
drinking water quality and public health in southwestern saudi arabia: the need for a national monitoring program.the aim was to investigate the bacteriological quality of drinking water, and explore the factors involved in the knowledge of the public about the quality of drinking water in najran region, saudi arabia.201725657607
sealing ability of a novel hydrophilic vs. conventional hydrophobic obturation systems: a bacterial leakage study.comparative assessment of apical sealing ability of a novel smart-seal system, resilon, and conventional gutta-percha system using a bacterial leakage model.201725657530
apical extrusion of enterococcus faecalis using three different rotary instrumentation techniques: an in vitro study.to compare the apical extrusion of enterococcus faecalis after instrumentation with three different ni-ti rotary instruments- an in vitro study.201725961619
antibacterial and smear layer removal capability of oregano extract solution.the aim of this in vitro study was to evaluate the antimicrobial effect of oregano extract solution (oes) against enterococcus faecalis within root canals and dentin tubules, and its effect on smear layer.201725713479
an assessment of antibacterial activity of three pulp capping materials on enterococcus faecalis by a direct contact test: an in vitro study.the aim of this in vitro study was to evaluate antimicrobial activities of three different pulp capping materials; biodentine, mineral trioxide aggregate (mta) angelus, and dycal against enterococcus faecalis and their durability with time.201726038657
bactericidal activity of propylene glycol, glycerine, polyethylene glycol 400, and polyethylene glycol 1000 against selected microorganisms.the aim of the present study was to evaluate the bactericidal activity of propylene glycol, glycerine, polyethylene glycol 400 (peg 400), and polyethylene glycol 1000 (peg 1000) against selected microorganisms in vitro.201725992336
comparison of fluid filtration and bacterial leakage techniques for evaluation of microleakage in endodontics.apical leakage assessment is a way to compare the efficiency of a filling material to seal the apical region of the tooth. many microleakage testing techniques have been introduced through the years, but there has been no agreement as to which technique gives the most accurate results. the aim of this study was to compare the accuracy of fluid filtration and bacterial leakage techniques in the assessment of the apical sealing ability of mineral trioxide aggregate (mta) and calcium enriched mixtu ...201725878674
quantitative evaluation of apical extrusion of intracanal bacteria using rotary protaper, k3xf, twisted and hand k-file system: an ex vivo study.the aim of this study was to evaluate the number of intracanal bacteria extruded apically during root canal preparation using rotary protaper, k3xf, twisted, and hand k-file system.201726481889
effect of passive ultrasonic irrigation on enterococcus faecalis from root canals: an ex vivo study.endodontic irrigation aims to clean and disinfect the root canal system. passive ultrasonic irrigation (pui) is based on the use of an ultrasound-activated instrument into the root canal filled with irrigant. the aim of this study was to evaluate, ex vivo, the effectiveness of pui in eliminating enterococcus faecalis from root canals. seventy-five extracted human single-root teeth were used. after root canal preparation, specimens were inoculated with e. faecalis and incubated at 37 °c for 21 da ...201726312969
reduction of enterococcus faecalis in curved root canals after various sizes and tapers of canal preparation.the aim of this study was to evaluate the reduction of enterococcus faecalis in curved root canals after various sizes and tapers of the canal preparation.201726180416
biofilm forming capacity of enterococcus faecalis on gutta-percha points treated with four disinfectants using confocal scanning laser microscope: an in vitro study.the aim of this study was to evaluate and compare the in vitro biofilm forming capacity of enterococcus faecalis on gutta-percha points disinfected with four disinfectants.201726288622
antibiotic resistance pattern of enterococci isolates from nosocomial infections in a tertiary care hospital in eastern india.resistance to commonly used antibiotics by enterococci causing nosocomial infections is of concern, which necessitates judicious, responsible and evidence-based use of antibiotics. the present study was conducted to review the prevalence and identify therapeutic options for nosocomial enterococcal infections in our tertiary care hospital.201726283837
phytochemical screening, antioxidant and antibacterial activities of extracts prepared from different tissues of schinus terebinthifolius raddi that occurs in the coast of bahia, brazil.schinus terebinthifolius is widely used in traditional medicine by brazilian quilombola and indigenous communities for treatment of several diseases. extracts from different tissues are being used to produce creams to treat cervicitis and cervicovaginitis. however, most studies are limited to the assessment of the essential oils and extracts obtained from the leaves.201726246739
antibacterial effect of calcium hydroxide combined with chlorhexidine on enterococcus faecalis: a systematic review and meta-analysis.enterococcus faecalis (e. faecalis) is the most frequently isolated strain in failed endodontic therapy cases since it is resistant to calcium hydroxide (ch). whether a combination of ch and chlorhexidine (chx) is more effective than ch alone against e. faecalis is a matter of controversy. thus, the aim of this study was to conduct a systematic review and meta-analysis of the literature.201725466470
in vitro activity of a polyhexanide-betaine solution against high-risk clones of multidrug-resistant nosocomial pathogens.to determine the in vitro activity of a polyhexanide-betaine solution against collection strains and multidrug-resistant (mdr) nosocomial isolates, including high-risk clones.201727004429
antimicrobial activity of aqueous and organic extracts of a saudi medicinal plant: rumex nervosus.the antimicrobial effect of aerial part of rumex nervosus obtained from the southern region of saudi arabia was evaluated on bacterial strains staphylococcus aureus, methicillin resistant s. aureus, enterococcus faecalis, escherichia coli, pseudomonas aeruginosa, and fungal strain candida albicans.201726681888
evaluation of minimal inhibitory concentration of two new materials using tube dilution method: an in vitro study.the aim of the study is to evaluate and compare the antimicrobial efficacy of two new materials mta plus and biodentine with proroot mta using tube dilution method.201725829698
microbiologic evaluation of matricaria and chlorhexidine against e. faecalis and c. albicans.to evaluate the antimicrobial activity of different concentrations of matricaria chamomilla and chlorhexidine gel against candida albicans and enterococcus faecalis.201726097333
comparative evaluation of antimicrobial efficacy of qmix™ 2 in 1, sodium hypochlorite, and chlorhexidine against enterococcus faecalis and candida albicans.the aim of this study is to compare the antimicrobial efficacy of qmix™ 2 in 1, sodium hypochlorite (naocl), and chlorhexidine (chx) against enterococcus faecalis and candida albicans.201725829691
in vitro antimicrobial activity of auxiliary chemical substances and natural extracts on candida albicans and enterococcus faecalis in root canals.the aim of this study was to evaluate the antimicrobial activity of auxiliary chemical substances and natural extracts on candida albicans and enterococcus faecalis inoculated in root canals.201723739849
procalcitonin levels in bloodstream infections caused by different sources and species of bacteria.the aim of this study was to evaluate procalcitonin (pct) diagnostic accuracy in discriminating gram-negative (gn) from gram-positive (gp) bloodstream infections and determining the relationship between pct levels, infection sites, and pathogen types.201727979420
branimycins b and c, antibiotics produced by the abyssal actinobacterium pseudonocardia carboxydivorans m-227.two new antibiotics, branimycins b (2) and c (3), were produced by fermentation of the abyssal actinobacterium pseudonocardia carboxydivorans m-227, isolated from deep seawater of the avilés submarine canyon. their structures were elucidated by hrms and nmr analyses. these compounds exhibit antibacterial activities against a panel of gram-positive bacteria, including corynebacterium urealyticum, clostridium perfringens, and micrococcus luteus, and against the gram-negative bacterium neisseria me ...201728169531
evaluation of the role of probiotics in endodontic treatment: a preliminary study.the principal goal of endodontics is the prevention of periapical infection. acute and chronic apical periodontitis occur due to the persistence of pathogenic microorganisms such as enterococcus faecalis and candida albicans harboring the root canal systems of the teeth. the concept of the use of probiotics in addressing endodontic disease is new and has not been studied adequately. on the basis of the success of probiotics in periodontal treatment, this preliminary work was performed (a) to eva ...201728316949
evaluation of antimicrobial and antifungal efficacy of chitosan as endodontic irrigant against enterococcus faecalis and candida albicans biofilm formed on tooth substrate.bacterial biofilms formed on the root canal wall are often difficult to remove. this study aimed to evaluate the cytotoxic effect and antibacterial efficacy of chitosan when used as root canal irrigant against e. faecalis and candida albicans biofilm formed on tooth substrate.201728298975
additive and reducing effects between calcium hydroxide and current irrigation solutions.microorganisms should be considered to have the major role in starting and perpetuation of pulpo-periapical diseases. using intracanal medicaments is necessary to gain a bacteria-free environment in the canal system. calcium hydroxide (abbreviated as ca(oh)2), which is the most commonly used medicament in endodontic therapy, has been shown to be effective against primary sources of infection; however, its effectiveness against some microorganisms, such as candida albicans and enterococcus faecal ...201728258273
influence of sucrose on growth and sensitivity of candida albicans alone and in combination with enterococcus faecalis and streptococcus mutans to photodynamic therapy.this study has evaluated the effects of photodynamic inactivation (pdi) using erythrosine as photosensitizer and green light-emitting diode (led) on biofilms of candida albicans alone and in combination with enterococcus faecalis and streptococcus mutans. we have also evaluated the effect of sucrose on biofilm formation and bacterial growth and sensitivity to pdi. biofilms were formed in suspension of 10(6) cells/ml on plates before being grown in broth culture with and without sucrose and incub ...201728389898
antimicrobial efficacy of probiotic-containing toothpastes: an in vitro evaluation.aim to evaluate, in vitro antimicrobial ability of two probiotic toothpastes (one containing lactobacillus paracasei, other containing lactobacillus acidophilus) and one toothpaste without probiotic separately, and in a combination with two different mouthrinses (one containing essential oils and the other containing hexitidine). methods antimicrobial susceptibility was checked by using the ditch method and clinical laboratory standard institute (clsi) guidelines. two different toothpastes with ...201728165440
biological activities of rosmarinus officinalis l. (rosemary) extract as analyzed in microorganisms and cells.r. officinalis l. is an aromatic plant commonly used as condiment and for medicinal purposes. biological activities of its extract were evaluated in this study, as antimicrobial effect on mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory capacity, and genotoxicity. monomicrobial biofilms of candida albicans, staphylococcus aureus, enterococcus faecalis, streptococcus mutans and pseudomonas aeruginosa and polymicrobial biofilms composed of c. albicans with each bacterium were form ...201728093936
antibacterial efficacy of azadirachta indica, mimusops elengi and 2% chx on multispecies dentinal biofilm.to check the antimicrobial activity of azadirachta indica (neem), mimusops elengi (bakul), and chlorhexidine gluconate (chx) on multispecies biofilm of common endodontic pathogens such as streptococcus mutans, enterococcus faecalis, staphylococcus aureus and candida albicans.201726752840
in vitro antimicrobial and cytotoxic effects of anacardium occidentale and mangifera indica in oral care.oral health is an integral and important component of general health. infectious diseases such as caries, periodontal, and gingivitis indicate the onset of imbalance in homeostasis between oral micro biota and host. the present day medicaments used in oral health care have numerous side effects. the uses of herbal plants as an alternative have gained popularity due to side effects of antibiotics and emergence of multidrug resistant strains. anacardium occidentale (cashew) and mangifera indica (m ...201725709341
antimicrobial activity of aspilia latissima (asteraceae).we evaluated the antimicrobial activity of aspilia latissima - an abundant plant from the brazilian pantanal region - against candida albicans, candida parapsilosis, candida krusei, candida tropicalis, pseudomonas aeruginosa, enterococcus faecalis, escherichia coli and staphylococcus aureus. the crude extracts and fractions showed activity in all tested microorganisms. the chloroform fraction of the leaves and roots showed the most antimicrobial activity against s. aureus, with an mic of 500 μg/ ...201726691468
[antibacterial and antifungal activity of salvia apiana against clinically important microorganisms].due to the great global concern regarding bacterial resistance to antibiotics, an ongoing search for new molecules having antibacterial activity is necessary. this study evaluated the antibacterial and anticandidal effects of a hexane extract from the root of salvia apiana. salvia extracts at concentrations of 27, 13.5, 6.8 and 3.4mg/ml caused growth inhibition of staphylococcus aureus, streptococcus pyogenes, enterococcus faecalis and candida albicans. however, no significant effect was observe ...201727595508
evaluation of antibacterial and antifungal activity of new calcium-based cement (biodentine) compared to mta and glass ionomer cement.to evaluate the antibacterial and antifungal properties of calcium-based cement, biodentine (ca3sio2), compared to commercial glass ionomer cements (gics) and mineral trioxide aggregate (mta).201725657526
development of a rapid identification method for the differentiation of enterococcus species using a species-specific multiplex pcr based on comparative genomics.enterococci are lactic acid bacteria that are commonly found in food and in animal gut. since 16 s ribosomal rna (rrna) sequences, genetic markers for bacterial identification, are similar among several enterococcus species, it is very difficult to determine the correct species based on only 16 s rrna sequences. therefore, we developed a rapid method for the identification of different enterococcus species using comparative genomics. we compared 38 genomes of 13 enterococcus species retrieved fr ...201728229213
antimicrobial resistance and virulence profile of enterococci isolated from poultry and cattle sources in nigeria.this study investigated the occurrence, antimicrobial resistance and virulence of enterococcus from poultry and cattle farms. three hundred and ninety samples: cloacal/rectal swabs (n = 260) and manure (n = 130] were processed for recovery of enterococcus species. standard bacteriological methods were used to isolate, identify and characterize enterococcus species for antimicrobial susceptibility and expression of virulence traits. detection of antibiotic resistance and virulence genes was carri ...201727987112
a 10-year profile of enterococcal bloodstream infections at a tertiary-care hospital in japan.the first aim of this study is to characterize the epidemiology of enterococcal bloodstream infections (bsis) at a japanese tertiary-care hospital. the second aim is to identify predictive factors for 30-day mortality.201728385565
safety assessment of commensal enterococci from dogs.enterococci form a complex, diverse, and very important group of bacteria from the technological and food safety aspect, or from the health-improving aspect as probiotics. generally, enterococci are considered to be of low pathogenic potential, which is associated mostly with clinical strains. in these strains, production of virulence factors as well as resistance to many antimicrobial drugs could complicate treatment of nosocomial infections. because there is a lack of information on incidence ...201728316009
maldi-tof mass spectrometry as a useful tool for identification of enterococcus spp. from wild birds and differentiation of closely related species.the aim of this study was to explore the accuracy and feasibility of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) in identifying bacteria from environmental sources, as compared to rpoa gene sequencing, and to evaluate the occurrence of bacteria of the genus enterococcus in wild birds. in addition, a phyloproteomic analysis of certain enterococcus species with spectral relationships was performed. the enterococci were isolated from 25 species of wil ...201728285496
identification and prevalence of tetracycline resistance in enterococci isolated from poultry in ilishan, ogun state, nigeria.tetracycline is one of the most frequently used antibiotics in nigeria both for human and animal infections because of its cheapness and ready availability. the use of tetracycline in animal husbandry could lead to horizontal transfer of tet genes from poultry to human through the gut microbiota, especially enterococci. therefore, this study is designed to identify different enterococcal species from poultry feces in selected farms in ilishan, ogun state, nigeria, determine the prevalence of tet ...201726957873
evaluation of dental adhesive systems incorporating an antibacterial monomer eugenyl methacrylate (egma) for endodontic restorations.the purpose of this study was to incorporate egma, an antibacterial monomer into two commercial dental adhesive systems for their application in endodontic restoration with the aim to disinfect the root canal space before curing and to inhibit bacterial growth on their surfaces after being cured.201728245928
n-acetylcysteine inhibits growth, adhesion and biofilm formation of gram-positive skin pathogens.n-acetylcysteine (nac) is a non-antibiotic drug with antimicrobial properties against biofilm phenotypes of gram-positive and gram-negative bacteria. our aim was to assess the effects of nac on the growth of gram-positive human skin and mucous membrane pathogens in the planktonic and biofilm phases. the minimum inhibitory concentrations (mics) of nac against enterococcus faecalis, corynebacterium ammoniagenes, mycobacterium smegmatis, propionibacterium acnes, staphylococcus aureus, s. epidermidi ...201728237766
prenylated flavonoids from the stems and roots of tripterygium wilfordii.phytochemical investigation on the stems and roots of tripterygium wilfordii led to the isolation and characterization of three new prenylated flavanones, tripteryols a-c (1-3), along with (±)-5,4'-dihydroxy-2'-methoxy-6',6″-dimethypyraro-(2″,3″:7,8)-6-methyflavanone (4), and ((2s)-5,7,4'-trihydroxy-2'-methoxy-8,5'-di(3-methyl-2-butenyl)-6-methylflavanone (5). structures of the compounds 1-5 were elucidated using spectroscopic techniques, such as uv, ir, nmr (1d and 2d), and hresi-ms. tripteryol ...201728389278
comparative genomics of enterococcus spp. isolated from bovine feces.enterococcus is ubiquitous in nature and is a commensal of both the bovine and human gastrointestinal (gi) tract. it is also associated with clinical infections in humans. subtherapeutic administration of antibiotics to cattle selects for antibiotic resistant enterococci in the bovine gi tract. antibiotic resistance genes (args) may be present in enterococci following antibiotic use in cattle. if located on mobile genetic elements (mges) their dissemination between enterococcus species and to pa ...201728270110
[profile of bacterial resistance in pediatric urinary tract infections in 2014].in pediatric units, bacteria-producing extended-spectrum-betalactamase (esbl) have an increasing prevalence among bacteria causing febrile urinary tract infections (utis). the purpose of this study was to evaluate the epidemiology of bacteria resistance patterns observed in utis, in order to assess the current antibiotic treatment protocols. this study is based upon a single-center retrospective chart review of the cytobacteriological urine cultures performed in utis between 1 january and 31 dec ...201728131557
effect of preoperative biliary drainage on bacterial flora in bile of patients with periampullary cancer.patients with obstructive jaundice due to periampullary tumours may undergo preoperative biliary drainage (pbd). the effect of pbd on the microbiome of the biliary system and on postoperative outcome remains unclear.201728121036
searching for a potential antibacterial lead structure against bacterial biofilms among new naphthoquinone compounds.the aims of this study were to design, synthesize and to evaluate 2-hydroxy-3-phenylsulfanylmethyl-[1,4]-naphthoquinones against gram-negative and gram-positive bacterial strains, including methicillin-resistant staphylococcus aureus (mrsa) and its biofilm, to probe for potential lead structures.201727930849
enterocin b3a-b3b produced by lab collected from infant faeces: potential utilization in the food industry for listeria monocytogenes biofilm management.enterococcus faecalis b3a-b3b produces the bacteriocin b3a-b3b with activity against listeria monocytogenes, staphylococcus aureus, methicillin-resistant staphylococcus aureus (mrsa) and clostridium perfringens, but apparently not against fungi or gram-negative bacteria, except for salmonella newport. b3a-b3b enterocin has two different nucleotides but similar amino acid composition to the class iib mr10a-mr10b enterocin. b3a-b3b consists of two peptides of predicted molecular mass of 5176.31 da ...201727878401
the safe enterocin dd14 is a leaderless two-peptide bacteriocin with anti-clostridium perfringens activity.enterococcus faecalis 14, a strain previously isolated from meconium, displayed activity against four clostridium perfringens isolates when co-cultured on agar plates. the anti-clostridium activity was ascribed to the production of enterocin dd14, which was subsequently purified. the minimum inhibitory concentration (mic) of enterocin dd14 against one collection strain and one clinical c. perfringens strain was determined at 50 µg/ml. furthermore, using the intestinal epithelial cell line ipec-1 ...201728104423
postmortem in vitro ethanol production-it could be more common than we think!the blood alcohol concentration (bac) is the most frequent determination in a forensic toxicology laboratory. despite its apparent simplicity, the results interpretation can be complex and always have relevant social and legal implications, particularly in postmortem analysis. in the present report we describe the case of a 55-year-old male with an apparent natural death by myocardial infarction, whose initial bac was 0.18g/l but, in repeated determinations prompted by discrepancies observed in ...201728108144
prevalence of multidrug resistant uropathogenic bacteria in pediatric patients of a tertiary care hospital in eastern india.today, because systemic infections such as urinary tract infection (uti) affect even pediatric patients, antibiotic resistant bacteria have become a constant clinical challenge. in the present study, a total of 1054 urine samples were collected from pediatric patients over 18 months. from these samples, 510 isolates of pathogenic bacteria were collected using hicrome uti agar. antibiotic sensitivity tests of isolates were performed using the kirby-bauer method. two gram-positive bacteria (entero ...201726617250
effect of zingiber officinale and propolis on microorganisms and endotoxins in root canals.the purpose of this study was to evaluate the effectiveness of glycolic propolis (pro) and ginger (gin) extracts, calcium hydroxide (ch), chlorhexidine (clx) gel and their combinations as icms (icms) against candida albicans, enterococcus faecalis, escherichia coli and endotoxins in root canals.201723559108
purification, characterization and functional analysis of the immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus and their antibiofilm properties.the present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus (pp-lec). the pp-lec was purified by affinity chromatography with mannose coupled sepharose cl-4b column and it exhibits single band with a molecular weight of 155 kda in sds-page. the surface morphology of purified pp-lec displays the homogeneous nature of protein. a distinct peak with a retention time of 3.3 min was appeared in high performance liqui ...201728110033
functional studies of e. faecalis rnase j2 and its role in virulence and fitness.post-transcriptional control provides bacterial pathogens a method by which they can rapidly adapt to environmental change. dual exo- and endonucleolytic activities of rnase j enzymes contribute to gram-positive rna processing and decay. first discovered in bacillus subtilis, rnase j1 plays a key role in mrna maturation and degradation, while the function of the paralogue rnase j2 is largely unknown. previously, we discovered that deletion of the enterococcus faecalis rnjb gene significantly att ...201728384222
cationic net charge and counter ion type as antimicrobial activity determinant factors of short lipopeptides.to get a better insight into the antimicrobial potency of short cationic lipopeptides, 35 new entities were synthesized using solid phase peptide strategy. all newly obtained lipopeptides were designed to be positively charged from +1 to +4. this was achieved by introducing basic amino acid - lysine - into the lipopeptide structure and had a hydrophobic fatty acid chain attached. lipopeptides were subjected to microbiological tests using reference strains of gram-negative bacteria: escherichia c ...201728203232
d-amino acids reduce enterococcus faecalis biofilms in vitro and in the presence of antimicrobials used for root canal treatment.enterococcus faecalis is the most frequent species present in post-treatment disease and plays a significant role in persistent periapical infections following root canal treatment. its ability to persist in stressful environments is inter alia, due to its ability to form biofilms. the presence of certain d-amino acids (daas) has previously been shown to reduce formation of bacillus subtilis biofilms. the aims of this investigation were to determine if daas disrupt biofilms in early and late gro ...201728151960
abietane diterpenes from the cones of abies numidica de lannoy ex carrière (pinaceae) and in vitro evaluation of their antimicrobial properties.eight known abietane diterpenes (1-8) were isolated for the first time from abies numidica cones (pinaceae). the structures of all compounds were established by means of 1d and 2d nmr, and uv spectral analyses. the hydromethanolic extract of a. numidica cones was tested for its antimicrobial activity against 17 gram-positive and gram-negative bacteria and against five yeasts by the use of liquid and solid medium and bioautography methods. the best antimicrobial activity was found against gram-po ...201727237830
a critical role for extracellular dna in dental plaque formation.extracellular dna (edna) has been identified in the matrix of many different monospecies biofilms in vitro, including some of those produced by oral bacteria. in many cases, edna stabilizes the structure of monospecies biofilms. here, the authors aimed to determine whether edna is an important component of natural, mixed-species oral biofilms, such as plaque on natural teeth or dental implants. to visualize edna in oral biofilms, approaches for fluorescently stained edna with either anti-dna ant ...201727770039
hydrocarbon-stapled lipopeptides exhibit selective antimicrobial activity.antimicrobial peptides (amps) occur widely in nature and have been studied for their therapeutic potential. amps are of interest due to the large number of possible chemical structural combinations using natural and unnatural amino acids, with varying effects on their biological activities. using physicochemical properties from known naturally occurring amphipathic cationic amps, several hydrocarbon-stapled lipopeptides (hslps) were designed, synthesized, and tested for antimicrobial properties. ...201728073163
combined chemical and physical transformation method with rbcl and sepiolite for the transformation of various bacterial species.dna transformation that delivers plasmid dnas into bacterial cells is fundamental in genetic manipulation to engineer and study bacteria. developed transformation methods to date are optimized to specific bacterial species for high efficiency. thus, there is always a demand for simple and species-independent transformation methods. we herein describe the development of a chemico-physical transformation method that combines a rubidium chloride (rbcl)-based chemical method and sepiolite-based phys ...201728185866
identification of the lyso-form n-acyl intramolecular transferase in low-gc firmicutes.bacterial lipoproteins are embedded in the cell membrane of both gram-positive and gram-negative bacteria where they serve numerous functions central to cell envelope physiology. lipoproteins are tethered to the membrane by an n-acyl-s-(mono/di)-acyl-glyceryl-cysteine anchor that is variably acylated depending on genera. in several low-gc gram-positive firmicutes, a monoacyl-glyceryl-cysteine with an n-terminal fatty acid (known as the lyso form) has been reported, though how it is formed is unk ...201728320885
the antibacterial activity of extracts of nine plant species with good activity against escherichia coli against five other bacteria and cytotoxicity of extracts.the development of antibiotic resistant bacteria stems from a number of factors, including inappropriate use of antibiotics in human and animal health and their prolonged use as growth promoters at sub-clinical doses in poultry and livestock production. we were interested in investigating plants that could be useful in protecting humans or animals against diarrhoea. we decided to work on extracts of nine plant species with good activity against escherichia coli based on earlier work in the phyto ...201728241818
epidihydropinidine, the main piperidine alkaloid compound of norway spruce (picea abies) shows promising antibacterial and anti-candida activity.this study reports for the first time promising antibacterial and antifungal effects of epidihydropinidine, the major piperidine alkaloid in the needles and bark of norway spruce, picea abies (l.) karsten. epidihydropinidine was growth inhibitory against all bacterial and fungal strains used in our investigation, showing the lowest mic value of 5.37μg/ml against pseudomonas aeruginosa, enterococcus faecalis, candida glabrata and c. albicans. epidihydropinidine was nearly three times more active ...201728163074
survival of vaginal microorganisms in three commercially available transport systems.transport systems are used to collect and maintain the viability of microorganisms. two amies media based transport systems, bd cultureswab™ maxv(+) amies medium without charcoal (maxv(+)) and fisherfinest(®) with amies gel transport medium without charcoal (fisherfinest(®)) were compared to a cary-blair media based transport system, starswab(®) anaerobic transport system (starswab(®)), for their capacity to maintain the viability of 17 clinical microorganisms commonly isolated from the vagina ( ...201728242337
antibacterial, antioxidant and anti-proliferative properties and zinc content of five south portugal herbs.crataegus monogyna l. (rosaceae) (cm), equisetum telmateia l. (equisataceae) (et), geranium purpureum vil. (geraniaceae) (gp), mentha suaveolens ehrh. (lamiaceae) (ms), and lavandula stoechas l. spp. luisieri (lamiaceae) (ls) are all medicinal.201727925492
evaluation of health potential of nutritionally enriched kodo millet (eleusine coracana) grown in himachal pradesh, india.in this study, kodo millet grains were phytochemically investigated for their nutritional and antioxidant potential for their use as functional foods. methanolic extracts of grains showed higher phenolic content and antioxidant activity. tlc studies of the extracted polyphenols from kodo millet showed the predominant presence of ferulic acid and cinnamic acid in the millet. further quantification of these polyphenols was done by using hplc, analysing ferulic acid and cinnamic acid. antagonistic ...201727507461
analysis of the expression of nlrp3 and aim2 in periapical lesions with apical periodontitis and microbial analysis outside the apical segment of teeth.to detect the distribution and expression levels of the nucleotide-binding oligomerization domain-like receptor protein 3 (nlrp3) and the absent in melanoma 2 (aim2) inflammasomes in periapical lesions and to analyse the possible microbial stimuli outside of teeth.201728193569
antibacterial activity of tribulus terrestris and its synergistic effect with capsella bursa-pastoris and glycyrrhiza glabra against oral pathogens: an in-vitro study.in this study, antimicrobial activities of an ethanol extract of tribulus terrestris aloneand in combination with capsella bursa-pastoris and glycyrrhiza glabra were examined in vitro against six pathogens namely streptococcus mutans, streptococcus sanguis, actinomyces viscosus, enterococcus faecalis staphylococcus aureus, and escherichia coli.201726101754
evaluating the effect of peptoid lipophilicity on antimicrobial potency, cytotoxicity, and combinatorial library design.growing prevalence of antibiotic resistant bacterial infections necessitates novel antimicrobials, which could be rapidly identified from combinatorial libraries. we report the use of the peptoid library agar diffusion (plad) assay to screen peptoid libraries against the eskape pathogens, including the optimization of assay conditions for each pathogen. work presented here focuses on the tailoring of combinatorial peptoid library design through a detailed study of how peptoid lipophilicity relat ...201728291947
fever and prodromal infections in anti-glomerular basement membrane disease.anti-glomerular basement membrane (gbm) disease is an autoimmune disorder with rapidly progressive glomerulonephritis and alveolar hemorrhage. fever symptom and prodromal infections have been reported in many cases, but still not been elucidated.201728261931
a study of pre-operative presence of micro-organisms in affected knee joints of rheumatoid arthritis patients who need total knee arthroplasty.to evaluate, by way of intraoperative tissue culture and pathological study, the pre-operative presence of micro-organisms in knee joints of patients with rheumatoid arthritis (ra) who need total knee arthroplasty (tka).201727919672
the evaluation of cultivable microbiota profile in patients with secondary endodontic infection before and after photo-activated disinfection.secondary/persistent endodontic infection can be the outcome of failure of endodontic treatment. photo-activated disinfection (pad) can be a useful adjunct to mechanical and antimicrobial agents in eliminating endopathogenic microorganisms. in this study, we evaluated the effect of pad on diversity and count of microbiota related to secondary/persistent endodontic infections.201728249746
novel bioactive root canal sealer to inhibit endodontic multispecies biofilms with remineralizing calcium phosphate ions.the objectives of this study were to: (1) develop a bioactive endodontic sealer via dimethylaminohexadecyl methacrylate (dmahdm), 2-methacryloyloxyethyl phosphorylcholine (mpc) and nanoparticles of amorphous calcium phosphate (nacp) for the first time; and (2) evaluate inhibition of early-stage and mature multispecies endodontic biofilm, bond strength to root canal dentine, and calcium (ca) and phosphate (p) ion release.201728223198
the use of bacteriophages to biocontrol oral biofilms.infections induced by oral biofilms include caries, as well as periodontal, and peri-implant disease, and may influence quality of life, systemic health, and expenditure. as bacterial biofilms are highly resistant and resilient to conventional antibacterial therapy, it has been difficult to combat these infections. an innovative alternative to the biocontrol of oral biofilms could be to use bacteriophages or phages, the viruses of bacteria, which are specific, non-toxic, self-proliferating, and ...201728108235
molecular viability testing of uv-inactivated bacteria.the polymerase chain reaction (pcr) is effective at detecting bacterial dna in samples, but it is unable to differentiate viable bacteria from inactivated cells or free dna fragments. new pcr-based analytical strategies have been developed to address this limitation. molecular viability testing (mvt) correlates bacterial viability with the ability to rapidly synthesize species-specific ribosomal rna precursor (pre-rrna) in response to brief nutritional stimulation. previous studies demonstrated ...201728283525
effects of synbiotics on immunity and disease resistance of narrow-clawed crayfish, astacus leptodactylus leptodactylus (eschscholtz, 1823).the aim of this study was to evaluate the effects of prebiotics (mannanoligosaccharide and xylooligosaccharide), probiotics (enterococcus faecalis and pediococcus acidilactici) and synbiotics for 126 days on the immune responses, hemolymph indices, antioxidant enzymes, and biological responses after a 48-hour aeromonas hydrophila exposure of sub-adult crayfish (11.45 ± 1.87 g). most antibacterial activities were observed in the shell mucus of crayfish fed a diet containing xylooligosaccharide +  ...201728363588
the influence of the autochthonous wastewater microbiota and gene host on the fate of invasive antibiotic resistance genes.the aim of this study was to assess the fate of invasive antibiotic resistance genes (arg) discharged in wastewater. with this objective, antibiotic resistant bacteria (arb) known to harbor specific arg were inoculated in wastewater (hospital effluent, or municipal raw and treated wastewater) and in ultra-pure sterile water microcosms. two sets of wastewater arb isolates were used - set 1, enterococcus faecalis, acinetobacter johnsonii, klebsiella pneumoniae and set 2, enterococcus faecium, acin ...201727697350
antimicrobial assay and genetic screening of selected freshwater cyanobacteria and identification of a biomolecule dihydro-2h-pyran-2-one derivative.explorations of freshwater cyanobacteria as antimicrobial (bacteria, fungi and methicillin-resistant staphylococcus aureus (mrsa) strains) drug resource using bioassay, nrps (non-ribosomal polypeptide synthetase) and pks (polyketide synthase) genes, as well as in silico approach.201728004519
antimicrobial properties of black grape (vitis vinifera l.) peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds.black grape peel possesses a substantial amount of polyphenolic antimicrobial compounds that can be used for controlling the growth of pathogenic microorganisms. the purpose of this study was to assess antibacterial and antifungal activity of black grape peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds, respectively.201726729960
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