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impacts of diurnal temperature and larval density on aquatic development of aedes aegypti.the increasing range of aedes aegypti, vector for zika, dengue, chikungunya, and other viruses, has brought attention to the need to understand the population and transmission dynamics of this mosquito. it is well understood that environmental factors and breeding site characteristics play a role in organismal development and the potential to transmit pathogens. in this study, we observe the impact of larval density in combination with diurnal temperature on the time to pupation, emergence, and ...201829513751
zika virus epidemiology in bolivia: a seroprevalence study in volunteer blood donors.zika virus (zikv), was widely reported in latin america and has been associated with neuropathologies, as microcephaly, but only few seroprevalence studies have been published to date. our objective was to determine the seroprevalence amongst bolivian blood donors and estimate the future potential circulation of the virus.201829513667
swift fabrication of ag nanostructures using a colloidal solution of holostemma ada-kodien (apocynaceae) - antibiofilm potential, insecticidal activity against mosquitoes and non-target impact on water bugs.recent research in entomology and parasitology focused on the efficacy of green fabricated nanomaterials as novel insecticides. in this study, we synthesized poly-dispersed and stable silver nanoparticles (agnps) using the leaf extract of holostemma ada-kodien. the nanostructures were characterized by ultraviolet-visible spectroscopy, fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, energy dispersive x-ray, and x-ray diffraction analysis. t ...201829510358
salivary factor ltrin from aedes aegypti facilitates the transmission of zika virus by interfering with the lymphotoxin-β receptor.pathogens have co-evolved with mosquitoes to optimize transmission to hosts. mosquito salivary-gland extract is known to modulate host immune responses and facilitate pathogen transmission, but the underlying molecular mechanisms of this have remained unknown. in this study, we identified and characterized a prominent 15-kilodalton protein, ltrin, obtained from the salivary glands of the mosquito aedes aegypti. ltrin expression was upregulated in blood-fed mosquitoes, and ltrin facilitated the t ...201829507355
transovarial transmission of dengue 1 virus in aedes aegypti larvae: real-time pcr analysis in a brazilian city with high mosquito population density.transovarial transmission is among the reported factors able to influence environmental maintenance of dengue virus (denv). endemic areas with active transmission of dengue are suitable for studying transovarial transmission. brazil is a country where dengue is endemic and where denv-1 is the most common disease-related virus serotype. this study aimed to identify transovarial transmission of denv-1 in aedes aegypti larvae by reverse-transcriptase nested real-time polymerase chain reaction. betw ...201829505732
wolbachia-mediated virus blocking in mosquito cells is dependent on xrn1-mediated viral rna degradation and influenced by viral replication rate.wolbachia is currently being developed as a novel tool to block the transmission of dengue viruses (denv) by aedes aegypti. a number of mechanisms have been proposed to explain the denv-blocking phenotype in mosquitoes, including competition for fatty acids like cholesterol, manipulation of host mirnas and upregulation of innate immune pathways in the mosquito. we examined the various stages in the denv infection process to better understand the mechanism of wolbachia-mediated virus blocking (wm ...201829494679
first evidence of vertical infection of dengue virus 2 in aedes aegypti mosquitoes from sinaloa, mexico.fourteen pools of aedes aegypti larvae collected within the urban area of culiacán, sinaloa, were analyzed by rt-pcr. the results demonstrate, for the first time, the vertical infection of serotype-2 dengue virus (denv-2) in sinaloa, mexico, suggesting that ae. aegypti acts as a natural reservoir of denv-2 in this region.201829485942
hypericum japonicum: a double-headed sword to combat vector control and cancer.mosquito control with naturally derived herbal insecticides has gained much momentum, with the increased insecticide resistance of vectors and the multiple infectious diseases spread by them. yet, recent studies also suggest that mosquitoes could probably transmit some cancerous cells or cancer-causing viruses from one individual to another between their blood meals. the current research thus focused on the screening and characterization of novel plants with both mosquitocidal and anticancerous ...201829476319
in tune with nature: wolbachia does not prevent pre-copula acoustic communication in aedes aegypti.mosquito-borne diseases are rapidly spreading to vast territories, putting at risk most of the world's population. a key player in this scenario is aedes aegypti, a hematophagous species which hosts and transmits viruses causing dengue and other serious illnesses. since vector control strategies relying only on insecticides have proven unsustainable, an alternative method involving the release of wolbachia-harboring individuals has emerged. its successful implementation vastly depends on how fit ...201829471864
dengue viruses and promising envelope protein domain iii-based vaccines.dengue viruses are emerging mosquito-borne pathogens belonging to flaviviridae family which are transmitted to humans via the bites of infected mosquitoes aedes aegypti and aedes albopictus. because of the wide distribution of these mosquito vectors, more than 2.5 billion people are approximately at risk of dengue infection. dengue viruses cause dengue fever and severe life-threatening illnesses as well as dengue hemorrhagic fever and dengue shock syndrome. all four serotypes of dengue virus can ...201829470620
using adult aedes aegypti females to predict areas at risk for dengue transmission: a spatial case-control study.traditional indices for measuring dengue fever risk in a given area are based on the immature forms of the vector (larvae and pupae surveys). however, this is inefficient because only adult female mosquitoes actually transmit the virus. based on these assumptions, our objective was to evaluate the association between an entomological index obtained from adult mosquito traps and the occurrence of dengue in a hyperendemic area. additionally, we compared its cost to that of the breteau index (bi).201829462598
seasonal distribution and container preference ratio of the dengue fever vector (aedes aegypti, diptera: culicidae) in rawalpindi, pakistan.aedes aegypti (l.) and aedes albopictus (skuse) are known vectors of dengue, chikungunya, and other pathogens; however, their ecology and role in virus transmission has not been well studied in pakistan. here, we report on an intensive survey of potential breeding sites of ae. aegypti in rawalpindi, punjab province, pakistan. the study continued for 11 mo and was divided into three seasons: january to june (pre-monsoon), july to september (monsoon), and october-november (post-monsoon). larval mo ...201829462424
improving vector-borne pathogen surveillance: a laboratory-based study exploring the potential to detect dengue virus and malaria parasites in mosquito saliva.vector-borne pathogen surveillance programmes typically rely on the collection of large numbers of potential vectors followed by screening protocols focused on detecting pathogens in the arthropods. these processes are laborious, time consuming, expensive, and require screening of large numbers of samples. to streamline the surveillance process, increase sample throughput, and improve cost-effectiveness, a method to detect dengue virus and malaria parasites (plasmodium falciparum) by leveraging ...201829460859
entomological and epidemiological aspects of dengue epidemics in fortaleza, ceará, brazil, 2001-2012.to characterize the entomological and epidemiological aspects of dengue epidemics occurred in fortaleza, ceará, brazil, from 2001 to 2012.201829451615
dynamic remodeling of lipids coincides with dengue virus replication in the midgut of aedes aegypti mosquitoes.we describe the first comprehensive analysis of the midgut metabolome of aedes aegypti, the primary mosquito vector for arboviruses such as dengue, zika, chikungunya and yellow fever viruses. transmission of these viruses depends on their ability to infect, replicate and disseminate from several tissues in the mosquito vector. the metabolic environments within these tissues play crucial roles in these processes. since these viruses are enveloped, viral replication, assembly and release occur on ...201829447265
mosquitoes of northwestern uganda.despite evidence of arbovirus activity in northwestern uganda (west nile sub-region), there is very limited information on the mosquito fauna of this region. the only published study reported 52 mosquito species in northwestern uganda but this study took place in 1950 and the information has not been updated for more than 60 yr. in january and june 2011, co2 baited-light traps were used to collect 49,231 mosquitoes from four different locations, paraa (9,487), chobe (20,025), sunguru (759), and ...201829444287
the impact of insecticide applications on the dynamics of resistance: the case of four aedes aegypti populations from different brazilian regions.in the tropics, the utilization of insecticides is still an important strategy for controlling aedes aegypti, the principle vector of dengue, chikungunya and zika viruses. however, increasing insecticide resistance in ae. aegypti populations might hinder insecticide efficacy on a long-term basis. it will be important to understand the dynamics and evolution of insecticide resistance by assessing its frequency and the mechanisms by which it occurs.201829432488
optimal control approach for establishing wmelpop wolbachia infection among wild aedes aegypti populations.wolbachia-based biocontrol has recently emerged as a potential method for prevention and control of dengue and other vector-borne diseases. major vector species, such as aedes aegypti females, when deliberately infected with wolbachia become less capable of getting viral infections and transmitting the virus to human hosts. in this paper, we propose an explicit sex-structured population model that describes an interaction of uninfected (wild) male and female mosquitoes and those deliberately inf ...201829429122
a natural agonist of mosquito trpa1 from the medicinal plant cinnamosma fragrans that is toxic, antifeedant, and repellent to the yellow fever mosquito aedes aegypti.plants produce various secondary metabolites that offer a potential source of novel insecticides and repellents for the control of mosquito vectors. plants of the genus cinnamosma are endemic to, and widely-distributed throughout, the island of madagascar. the barks of these species are commonly used in traditional medicines for treating a wide range of maladies. the therapeutic nature of the bark is thought to be associated with its enrichment of pungent drimane sesquiterpenes, which elicit ant ...201829425195
evidence for chikungunya and dengue transmission in quelimane, mozambique: results from an investigation of a potential outbreak of chikungunya virus.in january 2016, health authorities from zambézia province, mozambique reported the detection of some patients presenting with fever, arthralgia, and a positive result for chikungunya in an igm-based rapid diagnostic test (rdt). we initiated a study to investigate a potential chikungunya outbreak in the city of quelimane.201829415070
aedes aegypti antiviral adaptive response against denv-2.priming is the conceptual term defining memory phenomenon in innate immune response. numerous examples of enhanced secondary immune response have been described in diverse taxa of invertebrates; which naturally lacks memory response. in mosquitoes, a previous non-lethal challenge with some specific pathogens modify their immune response against the same microorganism; developing an improved antimicrobial reaction. in this work, we explore the ability of aedes aegypti to mount a higher antiviral ...201829408269
existing and potential infection risk zones of yellow fever worldwide: a modelling analysis.yellow fever cases are under-reported and the exact distribution of the disease is unknown. an effective vaccine is available but more information is needed about which populations within risk zones should be targeted to implement interventions. substantial outbreaks of yellow fever in angola, democratic republic of the congo, and brazil, coupled with the global expansion of the range of its main urban vector, aedes aegypti, suggest that yellow fever has the propensity to spread further internat ...201829398634
immune response-related genes associated to blocking midgut dengue virus infection in aedes aegypti strains that differ in susceptibility.aedes (stegomyia) aegypti, the principal global vector of dengue viruses, has differences in its' susceptibility to dengue virus infection. we compared the global expression of genes in the midguts of colombian ae. aegypti dengue-susceptible (cali-s) and dengue-refractory (cali-mib) field derived strains after ingesting either a sugarmeal, a bloodmeal, or a bloodmeal containing dengue virus serotype 2 (denv-2). microarray-based transcriptome analysis among treatments indicated a total of 4725 tr ...201829389079
aedes mosquitoes and aedes-borne arboviruses in africa: current and future threats.the zika crisis drew attention to the long-overlooked problem of arboviruses transmitted byaedesmosquitoes in africa. yellow fever, dengue, chikungunya and zika are poorly controlled in africa and often go unrecognized. however, to combat these diseases, both in africa and worldwide, it is crucial that this situation changes. here, we review available data on the distribution of each disease in africa, theiraedesvectors, transmission potential, and challenges and opportunities foraedescontrol. d ...201829382107
bacillus wiedmannii biovar thuringiensis: a specialized mosquitocidal pathogen with plasmids from diverse origins.bacillus cereus sensu lato also known as b. cereus group is composed of an ecologically diverse bacterial group with an increasing number of related species, some of which are medically or agriculturally important. numerous efforts have been undertaken to allow presumptive differentiation of b. cereus group species from one another. fcc41 is a bacillus sp. strain toxic against mosquito species like aedes aegypti, aedes (ochlerotatus) albifasciatus, culex pipiens, culex quinquefasciatus, and culex ...201830285095
using maldi-tof ms to identify mosquitoes collected in mali and their blood meals.matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) has been recently described as an innovative and effective tool for identifying arthropods and mosquito blood meal sources. to test this approach in the context of an entomological survey in the field, mosquitoes were collected from five ecologically distinct areas of mali. we successfully analysed the blood meals from 651 mosquito abdomens crushed on whatman filter paper (wfps) in the field using maldi-t ...201829409547
modulation of host learning in aedes aegypti mosquitoes.how mosquitoes determine which individuals to bite has important epidemiological consequences. this choice is not random; most mosquitoes specialize in one or a few vertebrate host species, and some individuals in a host population are preferred over others. mosquitoes will also blood feed from other hosts when their preferred is no longer abundant, but the mechanisms mediating these shifts between hosts, and preferences for certain individuals within a host species, remain unclear. here, we sho ...201829395917
the spatial and temporal scales of local dengue virus transmission in natural settings: a retrospective analysis.dengue is a vector-borne disease caused by the dengue virus (denv). despite the crucial role of aedes mosquitoes in denv transmission, pure vector indices poorly correlate with human infections. therefore there is great need for a better understanding of the spatial and temporal scales of denv transmission between mosquitoes and humans. here, we have systematically monitored the circulation of denv in individual aedes spp. mosquitoes and human patients from caratinga, a dengue endemic city in th ...201829394906
natural vertical transmission of dengue virus in aedes aegypti and aedes albopictus: a systematic review.dengue is of great concern in various parts of the world, especially in tropical and subtropical countries where the mosquito vectors aedes aegypti and aedes albopictus are present. the transmission of this virus to humans, by what is known as horizontal transmission, occurs through the bite of infected females of one or other of the two mosquito species. furthermore, an infected female or male parent, by what is known as vertical transmission, can transfer this arbovirus to some part of their o ...201829391071
altered vector competence in an experimental mosquito-mouse transmission model of zika infection.few animal models of zika virus (zikv) infection have incorporated arthropod-borne transmission. here, we establish an aedes aegypti mosquito model of zikv infection of mice, and demonstrate altered vector competency among three strains, (orlando, orl, ho chi minh, hcm, and patilas, pat). all strains acquired zikv in their midguts after a blood meal from infected mice, but zikv transmission only occurred in mice fed upon by hcm, and to a lesser extent pat, but not orl, mosquitoes. this defect in ...201829505571
effects of climate change on the spread of zika virus: a public health threat.zika is a vector-borne viral disease transmitted to humans primarily by aedes aegypti mosquitoes. the increased climate instability has contributed to the emergence of infections carried by mosquitoes like dengue, chikungunya and zika. while infection with the zika virus is not new, the recent epidemic of microcephaly in brazil and other countries in south america resulting from the infection of pregnant women with the zika virus raise a number of serious public health concerns. these include th ...201829500926
vector competence of aedes bromeliae and aedes vitattus mosquito populations from kenya for chikungunya virus.kenya has experienced outbreaks of chikungunya in the past years with the most recent outbreak occurring in mandera in the northern region in may 2016 and in mombasa in the coastal region from november 2017 to february 2018. despite the outbreaks in kenya, studies on vector competence have only been conducted on aedes aegypti. however, the role played by other mosquito species in transmission and maintenance of the virus in endemic areas remains unclear. this study sought to determine the possib ...201830321181
functional characterization of mosquito short neuropeptide f receptors.mosquito blood feeding transiently inhibits sugar- and host seeking through neuropeptide signaling. short neuropeptide f (snpf) is one of the neuromodulators involved in this regulation. here, we identified the genes for the snpf precursor and the snpf receptor in the southern house mosquito, culex quinquefasciatus. comparative analyses are made with the genes of the snpf precursor and receptor from two other important vectors, aedes aegypti and anopheles coluzzii. we functionally characterized ...201829550617
quantification through tlc-densitometric analysis, repellency and anticholinesterase activity of the homemade extract of indian cloves.the rise of the mosquitoes-transmitted diseases, like dengue, zika and chikungunya in brazil in the last years has increased concerns on protection against mosquitoes bites. however, the prohibitive prices of the commercially available repellents for the majority of the brazilian population has provoked a search for cheaper solutions, like the use of the homemade ethanolic extract of indian clove (syzygium aromaticum l.) as repellent, which has been reported as quite efficient by the local press ...201828913959
positional stable isotope tracer analysis reveals carbon routes during ammonia metabolism of aedes aegypti mosquitoes.in aedes aegypti females, the ammonia released during blood meal digestion is partially metabolized to facilitate the disposal of excess nitrogen. in this study, we used low- and high-resolution liquid chromatography-mass spectrometry (lc/ms) techniques to investigate the role of glucose during ammonia detoxification. mosquitoes were fed a blood meal supplemented with [1,2-13c2]glucose, and downstream metabolites were measured for 24 h. quantification of [13c] amino acids in the entire mosquito ...201828970248
zika and chikungunya virus detection in naturally infected aedes aegypti in ecuador.the wide and rapid spread of chikungunya (chikv) and zika (zikv) viruses represent a global public health problem, especially for tropical and subtropical environments. the early detection of chikv and zikv in mosquitoes may help to understand the dynamics of the diseases in high-risk areas, and to design data based epidemiological surveillance to activate the preparedness and response of the public health system and vector control programs. this study was done to detect zikv and chikv viruses i ...201828982578
screening of plant extracts and fractions on aedes aegypti larvae found in the state of mato grosso do sul (linnaeus, 1762) (culicidae).the constant use of chemical insecticides for aedes aegypti control has caused resistance in the mosquito populations. thus, the objective of this study was to analyze the larvicidal potential of extracts and fractions of plants on a. aegypti larvae. the analysis included sixty one extracts and twenty five fractions of fifty botanical species at concentrations of 0.25; 0.12; 0.06 to 0.03 mg ml-1; 4 replications and one negative control of dechlorinate water and 1% dmso; and a positive control wi ...201828640344
synthesis and structure-activity relationships of carbohydrazides and 1,3,4-oxadiazole derivatives bearing an imidazolidine moiety against the yellow fever and dengue vector, aedes aegypti.1,3,4-oxadiazole and imidazolidine rings are important heterocyclic compounds exhibiting a variety of biological activities. in this study, novel compounds with oxadiazole and imidazolidine rings were synthesized from 3-(methylsulfonyl)-2-oxoimidazolidine-1-carbonyl chloride and screened for insecticidal activities. the proposed structures of the 17 synthesized compounds were confirmed using elemental analysis, infrared (ir), proton nuclear magnetic resonance (1 h-nmr), and mass spectroscopy.201828869331
transcription profiling for defensins of aedes aegypti (diptera: culicidae) during development and in response to infection with chikungunya and zika viruses.aedes aegypti (l.) is a vector of chikungunya, dengue, yellow fever and zika viruses. these viruses encounter a variety of induced defense responses from the innate immune system of the mosquito. we cloned defensin a from ae. aegypti using laboratory populations originating from key west and orlando, florida. to characterize inducible immune defensin peptides, we examined the defensin a (defa) and defensin c (defc) expression through time course studies using quantitative real-time pcr. we obser ...201828968775
first evidence of zika virus venereal transmission in aedes aegypti mosquitoes.aedes aegypti is considered the main zika virus (zikv) vector, and is thought to be responsible for the 2015-2016 outbreak in brazil. zika positive ae. aegypti males collected in the field suggest that vertical and/or venereal transmission of zikv may occur.201829185595
point-of-use nanobiosensor for detection of dengue virus ns1 antigen in adult aedes aegypti: a potential tool for improved dengue surveillance.dengue virus (denv) and its primary mosquito vectors aedes spp. have spread to every continent except antarctica, causing outbreaks and autochthonous transmission in previously disease-free regions. recently, the spread of other arboviruses carried by invasive aedes spp., such as chikungunya and zika, seem to be following similar trends as denv and have renewed interest in monitoring and modeling arboviruses within mosquito vectors. unfortunately, current commercially available detection methods ...201829182305
mapping the spatial distribution of aedes aegypti and aedes albopictus.mosquito-borne infectious diseases, such as rift valley fever, dengue, chikungunya and zika, have caused mass human death with the transnational expansion fueled by economic globalization. simulating the distribution of the disease vectors is of great importance in formulating public health planning and disease control strategies. in the present study, we simulated the global distribution of aedes aegypti and aedes albopictus at a 5×5km spatial resolution with high-dimensional multidisciplinary ...201829191515
nanotitania crystals induced efficient photocatalytic color degradation, antimicrobial and larvicidal activity.textile industries release tonnes of harmful toxic dyes into the environment, causing severe effects on living organisms, including humans. mosquitoes vectors spread important diseases which cause millions of human deaths worldwide. to control mosquitoes a number of synthetic mosquitocidal agents have been employed but all these pesticides pose harmful effects to human health and non-target species and also led to resistance development in treated vectors. microbial strains are also developing r ...201829241121
[dengue, zika, chikungunya and the development of vaccines].dengue (denv), zika (zikv) and chikungunya (chikv), three arbovirosis transmitted by aedes mosquitoes, have spread in recent decades in humid tropical and subtropical zones. dengue is epidemic in subtropical areas of argentina. denv infection confers lasting immunity against the infecting serotype but increases the risk of serious disease upon reinfection by any of the other three. the recombinant tetravalent vaccine dengvaxia® prevents severe dengue and hospitalization in seropositive subjects. ...201829360072
cytoplasmic glutamine synthetase gene expression regulates larval development in bactrocera dorsalis (hendel).in insects, glutamine synthetase (gs), a key enzyme in the synthesis of glutamine, has been reported to be associated with embryonic development, heat shock response, and fecundity regulation. however, little is known about the influence of gs on postembryonic development. in this study, we demonstrate that blocking the activity of gs in the oriental fruit fly (bactrocera dorsalis) with use of a gs-specific inhibitor (l-methionine s-sulfoximine), led to a significant delay in larval development, ...201829359358
at the origin of a worldwide invasion: unraveling the genetic makeup of the caribbean bridgehead populations of the dengue vector aedes aegypti.human-driven global environmental changes have considerably increased the risk of biological invasions, especially the spread of human parasites and their vectors. among exotic species that have major impacts on public health, the dengue fever mosquito aedes aegypti originating from africa has spread worldwide during the last three centuries. although considerable progress has been recently made in understanding the history of this invasion, the respective roles of human and abiotic factors in s ...201829267872
green synthesis of silver nanoparticles using holarrhena antidysenterica (l.) wall.bark extract and their larvicidal activity against dengue and filariasis vectors.the present study was carried out to evaluate the larvicidal potential of methanol, hexane, acetone, chloroform, and aqueous bark extracts of holarrhena antidysenterica (l.) wall. and silver nanoparticles (agnps) synthesized using aqueous bark extract against the third instar larvae of aedes aegypti l. and culex quinquefasciatus say. agnps were prepared by adding 10 ml of aqueous bark extract in 90 ml of 1 mm silver nitrate (agno3) solution. after 5 min of mixing, a change in color from yellow t ...201829250727
field- and clinically derived estimates of wolbachia-mediated blocking of dengue virus transmission potential in aedes aegypti mosquitoes.the wmel strain of wolbachia can reduce the permissiveness of aedes aegypti mosquitoes to disseminated arboviral infections. here, we report that wmel-infected ae. aegypti (ho chi minh city background), when directly blood-fed on 141 viremic dengue patients, have lower dengue virus (denv) transmission potential and have a longer extrinsic incubation period than their wild-type counterparts. the wmel-infected mosquitoes that are field-reared have even greater relative resistance to denv infection ...201829279375
assessment of a trap based aedes aegypti surveillance program using mathematical modeling.the goal of this study was to assess the goodness-of-fit of theoretical models of population dynamics of aedes aegypti to trap data collected by a long term entomological surveillance program. the carrying capacity k of this vector was estimated at city and neighborhood level. adult mosquito abundance was measured via adults collected weekly by a network of sticky traps (mosquitraps) from january 2008 to december 2011 in vitória, espírito santo, brazil. k was the only free parameter estimated by ...201829304070
hypoxia-induced transcription factor signaling is essential for larval growth of the mosquito aedes aegypti.gut microbes positively affect the physiology of many animals, but the molecular mechanisms underlying these benefits remain poorly understood. we recently reported that bacteria-induced gut hypoxia functions as a signal for growth and molting of the mosquito aedes aegypti in this study, we tested the hypothesis that transduction of a gut hypoxia signal requires hypoxia-induced transcription factors (hifs). expression studies showed that hif-α was stabilized in larvae containing bacteria that in ...201829298915
group b wolbachia strain-dependent inhibition of arboviruses.mosquito-borne viruses, including zika virus (zikv) and dengue virus (denv), are global threats that continue to infect millions annually. historically, efforts to combat the spread of these diseases have sought to eradicate the mosquito population. this has had limited success. recent efforts to combat the spread of these diseases have targeted the mosquito population and the mosquito's ability to transmit viruses by altering the mosquito's microbiome. the introduction of particular strains of ...201829297702
zika virus infection modulates the bacterial diversity associated with aedes aegypti as revealed by metagenomic analysis.zika is a re-emerging infection that has been considered a major threat to global public health. currently at least 100 countries are at risk of zika virus (zikv) transmission. aedes aegypti is the main mosquito vector in the americas. this vector is exposed to, and interacts symbiotically with a variety of microorganisms in its environment, which may result in the formation of a lifetime association. here, the unknown effect that zikv exerts on the dynamic bacterial community harbored by this m ...201829293631
insecticide treated curtains and residual insecticide treatment to control aedes aegypti: an acceptability study in santiago de cuba.within the context of a field trial conducted by the cuban vector control program (aacp), we assessed acceptability of insecticide-treated curtains (itcs) and residual insecticide treatment (rit) with deltamethrin by the community. we also assessed the potential influence of interviewees' risk perceptions for getting dengue and disease severity.201829293501
n-substituted methyl maleamates as larvicidal compounds against aedes aegypti (diptera: culicidae).severe human arboviral diseases can be transmitted by the mosquito aedes aegypti (l.), including dengue, chikungunya, zika, and yellow fever. the use of larvicides in containers that can result as potential breeding places and cannot be eliminated is the main alternative in control programs. however, their continuous and widespread use caused an increase in insecticide-resistant populations of this mosquito. the aim of this study was to evaluate the effect of three n-substituted methyl maleamate ...201829290037
fine-scale landscape genomics helps explain the slow spatial spread of wolbachia through the aedes aegypti population in cairns, australia.the endosymbiotic bacterium wolbachia suppresses the capacity for arbovirus transmission in the mosquito aedes aegypti, and can spread spatially through wild mosquito populations following local introductions. recent introductions in cairns, australia have demonstrated slower than expected spatial spread. potential reasons for this include: (i) barriers to ae. aegypti dispersal; (ii) higher incidence of long-range dispersal; and (iii) intergenerational loss of wolbachia. we investigated these th ...201829358725
current concerns and perspectives on zika virus co-infection with arboviruses and hiv.dissemination of vector-borne viruses, such as zika virus (zikv), in tropical and sub-tropical regions has a complicated impact on the immunopathogenesis of other endemic viruses such as dengue virus (denv), chikungunya virus (chikv) and human immunodeficiency virus (hiv). the consequences of the possible co-infections with these viruses have specifically shown significant impact on the treatment and vaccination strategies. zikv is a mosquito-borne flavivirus from african and asian lineages that ...201829352633
aerial ulv control of aedes aegypti with naled (dibrom) inside simulated rural village and urban cryptic habitats.we conducted aerial fixed wing ultra low volume (ulv) spray trials with naled to investigate penetration of exposed and simulated cryptic habitat within opened buildings, partially sealed buildings, and outdoor locations targeting sentinel adult aedes aegypti mosquitoes in north central florida. mortality was observed in open and closed buildings and outdoors, even in mosquitoes placed in cryptic habitats. observations on the impact of building type, mosquito exposure method such as placement in ...201829352307
reduction in musca domestica fecundity by dsrna-mediated gene knockdown.house flies (musca domestica) are worldwide agricultural pests with estimated control costs at $375 million annually in the u.s. non-target effects and widespread resistance challenge the efficacy of traditional chemical control. double stranded rna (dsrna) has been suggested as a biopesticide for m. domestica but a phenotypic response due to the induction of the rnai pathway has not been demonstrated in adults. in this study female house flies were injected with dsrna targeting actin-5c or ribo ...201829342168
zika virus in salivary glands of five different species of wild-caught mosquitoes from mexico.zika virus (zikv) is a mosquito-borne pathogen, and aedes aegypti has been identified as the main vector of the disease. other mosquito species in the aedes and culex genera have been suggested to have the potential for being competent vectors based on experimental exposition of mosquitoes to an infectious blood meal containing zikv. here, we report the isolation in cell culture of zikv obtained from different body parts of wild-caught female mosquitoes (ae. aegypti, ae. vexans, cx. quinquefasci ...201829339746
wolbachia-induced transcription factor gata4 suppresses ovary-specific genes blastoderm-specific protein 25d and imaginal disc growth factor.the endosymbiotic bacterium wolbachia infects a wide array of insect hosts and has been implicated in a range of biological modifications as a consequence of its infection. previously, it was shown that the transcription factor gata4 was significantly induced in wolbachia wmelpop-cla strain infected aedes aegypti whole mosquitoes and cells. here, we provide evidence that this induction also occurs in mosquito ovaries where the ovary-specific genes blastoderm-specific protein 25d (bsg25d) and ima ...201829336504
vibrational properties of bulk boric acid 2a and 3t polymorphs and their two-dimensional layers: measurements and density functional theory calculations.boric acid (h3bo3) is being used effectively nowadays in traps/baits for the management of aedes aegypti l. and aedes albopictus skuse species of mosquitoes, which are the main spreading vectors worldwide for diseases such as malaria, dengue, and zika. previously, we published results on the structural, electronic, and optical properties of its molecular triclinic h3bo3-2a and trigonal h3bo3-3t polymorphs within the framework of density functional theory (dft). because of the renewed importance ...201829328646
the discovery of arthropod-specific viruses in hematophagous arthropods: an open door to understanding the mechanisms of arbovirus and arthropod evolution?the discovery of an odd virus from hematophagous arthropods 40 years ago by stollar and thomas described cell fusing agent virus in cells derived from aedes aegypti mosquitoes. then came the report of kamiti river virus from ae. macintoshi in 1999, followed by worldwide reports of the discovery of other viruses of mosquitoes, ticks, and midges that replicate only in arthropods and not in vertebrates or in vertebrate cells. these viruses (now totaling at least 64 published) have genomes analogous ...201829324047
dengue hemorrhagic fever virus in saudi arabia: a review.dengue fever is a global disease with a spectrum of clinical manifestation ranging from mild febrile disease to a severe disease in the form of dengue hemorrhagic fever and dengue shock syndrome. dengue virus is one viral hemorrhagic fever that exists in the kingdom of saudi arabia in addition to alkhurma (alkhurma) hemorrhagic fever, chikungunya virus, crimean-congo hemorrhagic fever, and rift valley fever. the disease is limited to the western and south-western regions of saudi arabia, where a ...201829319426
qsar analysis of plant-derived compounds with larvicidal activity against zika aedes aegypti (diptera: culicidae) vector using freely available descriptors.we develop a qsar model for predicting the larvicidal activity of 60 plant-derived molecules against aedes aegypti l. (diptera: culicidae), vector of several diseases such as, dengue, yellow fever, chikungunya and zika virus. the balanced subsets method (bsm) based on k-means cluster analysis (k-mca) is employed to split the data set. the replacement method (rm) variable subset selection technique coupled with multivariable linear regression (mlr) proves to be successful for exploring 18,326 mol ...201829314584
krüppel homologue 1 acts as a repressor and an activator in the transcriptional response to juvenile hormone in adult mosquitoes.krüppel homologue 1 (kr-h1) is a zinc finger transcription factor that is upregulated in insects by juvenile hormone (jh) in metamorphosis and adult reproduction. the molecular function of kr-h1 in reproduction remains largely unknown. here we report that aakr-h1 functions as an important transcription regulator in adult female aedes aegypti mosquitoes. the amount of aakr-h1 protein increases with rising jh levels after adult emergence, reaches its peak at 48 h after eclosion, then decreases gra ...201829314423
aristololactam derivatives from the fruits of aristolochia contorta bunge.three new aristololactam derivatives, aristololactam w-y (1-3), and three known compounds (4-6) were isolated from the fruits of aristolochia contorta bunge. compounds 1 and 2 represent the first example of an n-ch2och3 aristololactam derivative from natural products. their structures were elucidated by 1d/2d nmr and hresims spectra. all of the isolated compounds were evaluated for their insecticidal activity against 4th instar larvae of aedes aegypti. compound 4 displayed insecticidal activity ...201829313366
the genomes of four meyerozymacaribbica isolates and novel insights into the meyerozymaguilliermondii species complex.yeasts of the meyerozyma guilliermondii species complex are widespread in nature and can be isolated from a variety of sources, from the environment to arthropods to hospital patients. to date, the species complex comprises the thoroughly studied and versatile m. guilliermondii, and the hard to distinguish m. caribbica, and candida carpophila here we report the whole-genome sequencing and de novo assembly of four m. caribbica isolates, identified with the most recent molecular techniques, derive ...201829311113
critical low temperature for the survival of aedes aegypti in taiwan.taiwan is geographically located in a region that spans both tropical and subtropical climates (22-25°n and 120-122°e). the taiwan centers for disease control have found that the ecological habitat of aedes aegypti appears only south of 23.5°n. low temperatures may contribute to this particular habitat distribution of ae. aegypti under the influence of the east asian winter monsoon. however, the threshold condition related to critically low temperatures remains unclear because of the lack of lar ...201829310716
multifaceted functional implications of an endogenously expressed trna fragment in the vector mosquito aedes aegypti.the mosquito aedes aegypti is the primary vector of human arboviral diseases caused by dengue, chikungunya and zika viruses. many studies have shown the potential roles of small rna molecules such as microrna, small interfering rna and piwi-interacting rna in vector mosquitoes. the function of trna fragments (trf), the newly discovered class of small rnas, in mosquitoes is not known. in this study, we show that specific trfs are expressed in significantly differential manner between males and fe ...201829364883
the wolbachia strain wau provides highly efficient virus transmission blocking in aedes aegypti.introduced transinfections of the inherited bacteria wolbachia can inhibit transmission of viruses by aedes mosquitoes, and in ae. aegypti are now being deployed for dengue control in a number of countries. only three wolbachia strains from the large number that exist in nature have to date been introduced and characterized in this species. here novel ae. aegypti transinfections were generated using the walba and wau strains. in its native ae. albopictus, walba is maintained at lower density tha ...201829370307
mosquito vector-associated microbiota: metabarcoding bacteria and eukaryotic symbionts across habitat types in thailand endemic for dengue and other arthropod-borne diseases.vector-borne diseases are a major health burden, yet factors affecting their spread are only partially understood. for example, microbial symbionts can impact mosquito reproduction, survival, and vectorial capacity, and hence affect disease transmission. nonetheless, current knowledge of mosquito-associated microbial communities is limited. to characterize the bacterial and eukaryotic microbial communities of multiple vector species collected from different habitat types in disease endemic areas ...201829375803
environmentally benign nanometric neem-laced urea emulsion for controlling mosquito population in environment.the increasing risk of vector-borne diseases and the environmental pollution in the day-to-day life due to the usage of the conventional pesticides makes the role of nanotechnology to come into the action. the current study deals with one of the applications of nanotechnology through the formulation of neem urea nanoemulsion (nune). nune was formulated using neem oil, tween 20, and urea using the microfluidization method. prior to the development of nanoemulsion, the ratio of oil/surfactant/urea ...201829116538
functional characterization of the dual allatostatin-a receptors in mosquitoes.the neuropeptide allatostatin-a (asta) and its cognate receptors (astars) are involved in the modulation of feeding behavior, which in hematophagous insects includes the regulation of the disease vector-related behaviors, host seeking and blood feeding. in mosquitoes and other dipterans, there are two copies of astar, contrasting with the single copy found in other insects. in this study, we identified and cloned the dual astar system of two important disease vectors aedes aegypti and culex quin ...201829103918
genome sequence of a novel strain of a phasi charoen-like virus identified in zhanjiang, china.here, we report the genome sequence of a novel strain of a phasi charoen-like virus, isolated from aedes aegypti mosquitoes caught in zhanjiang province of china. phylogenetic analysis suggested that this virus belongs to a new genus, goukovirus, in the family bunyaviridae this is the first reported genome sequence of a phasi charoen-like virus identified in china.201829326198
behavior of detoxifying enzymes of aedes aegypti exposed to girgensohnine alkaloid analog and cymbopogon flexuosus essential oil.because mosquito control depend on the use of commercial insecticides and resistance has been described in some of them, there is a need to explore new molecules no resistant. in vivo effects of girgensohnine analog 2-(3,4-dimethoxyphenyl)-2-(piperidin-1-yl)acetonitrile dppa and cymbopogon flexuosus essential oil cfeo, on the detoxifying enzymes acetylcholinesterase (ache), glutathione-s-transferase (gst), nonspecific esterases (α- and β-), mixed function oxidases (mfo) and p-npa esterases were ...201829129589
functional characterization of a serine protease inhibitor modulated in the infection of the aedes aegypti with dengue virus.during feeding with blood meal, female aedes aegypti can transmit infectious agents, such as dengue, yellow fever, chikungunya and zika viruses. dengue virus causes human mortality in tropical regions of the world, and there is no specific treatment or vaccine with maximum efficiency being used for these infections. in the vector-virus interaction, the production of several molecules is modulated by both mosquitoes and invading agents. however, little information is available about these molecul ...201829133118
zika virus: what we need to know?zika virus is one of the emerging viruses and is of significant threat to human health globally. it is a mosquito borne flavivirus similar to dengue, yellow fever, and west nile viruses. it was reported about 5 decades ago and then it spreads to different parts of the world. large outbreaks were reported on yap islands in 2007. now it has gained wide attention globally by health communities. major vector for virus transmission is aedes aegypti mosquito. zikv infection is mostly asymptomatic but ...201829131357
bacterial exopolysaccharide (eps)-coated zno nanoparticles showed high antibiofilm activity and larvicidal toxicity against malaria and zika virus vectors.in this study, a novel and effective approach was performed to synthesize zno nanoparticles (zno nps) using the exopolysaccharides (eps) from the probiotic strain bacillus licheniformis dahb1. eps acted as reducing and stabilizing agent for the formation of eps-zno nps by co-precipitation method. structural characterization was investigated by a surface plasma resonance centered at 375nm in uv-vis spectrum. ftir spectrum exhibited functional groups with strong absorption peak at 3814.7-420cm-1. ...201829173489
facile green synthesis of zinc oxide nanoparticles using ulva lactuca seaweed extract and evaluation of their photocatalytic, antibiofilm and insecticidal activity.the bioactivity of semiconductor nanocomplexes has been poorly studied in the field of pesticide science. in this research, the synthesis of zinc nanoparticles was accomplished through new effortless green chemistry process, using the ulva lactuca seaweed extract as a reducing and capping agent. the production of u. lactuca-fabricated zno nanoparticles (ul-zno nps) was characterized by powder x-ray diffraction (xrd), uv-visible, fourier transform infrared (ftir) spectroscopy, selected area elect ...201829169140
complex wolbachia infection dynamics in mosquitoes with imperfect maternal transmission.dengue, malaria, and zika are dangerous diseases primarily transmitted by aedes aegypti, aedes albopictus, and anopheles stephensi. in the last few years, a new disease control method, besides pesticide spraying to kill mosquitoes, has been developed by releasing mosquitoes carrying bacterium wolbachia into the natural areas to infect the wild population of mosquitoes and block disease transmission. the bacterium is transmitted by infected mothers and the maternal transmission was assumed to be ...201829161848
molecular detection and characterization of wolbachia pipientis from culex quinquefasciatus collected from lahore, pakistan.the gram-negative, pleomorphic endosymbiont wolbachia is known to infect a large number of insects and other arthropods naturally. this bacterium modifies the host biology, mainly causing reproductive alterations including feminization, death of male, parthenogenesis, and importantly cytoplasmic incompatibility. wolbachia-induced cytoplasmic incompatibility results in nonviable offspring and vector population suppression. in addition, this bacterium rapidly spreads and propagates within the host ...201829141766
potential role for flubendazole in limiting filariasis transmission: observations of microfilarial sensitivity.flubendazole (flbz) is a potent and efficacious macrofilaricide after parenteral administration. studies in animal models and one trial in patients infected with onchocerca volvulus revealed that flbz elicits minimal effects on microfilariae (mf). severe complications after ivermectin (ivm) treatment of patients with high loa loa microfilaraemia are of great concern. we examined the potential of flbz to rapidly kill l. loa mf, the phenomenon proposed to underlie the complications. mf of l. loa w ...201829141764
the effect of larval diet on adult survival, swarming activity and copulation success in male aedes aegypti (diptera: culicidae).control of aedes aegypti (l.) (diptera: culicidae) populations is vital for reducing the transmission of several pervasive human diseases. the success of new vector control technologies will be influenced by the fitness of laboratory-reared transgenic males. however, there has been relatively little published data on how rearing practices influence male fitness in aedes mosquitoes. in the laboratory, the effect of larval food availability on adult male fitness was tested, using a range of differ ...201829029298
diurnal temperature range and chikungunya virus infection in invasive mosquito vectors.climate strongly influences the geographic distribution and timing of mosquito-borne disease outbreaks. environmental temperature affects phenotypic traits of mosquitoes including vector competence for arboviruses mediated by changes in infection, extrinsic incubation period and in rates of transmission. most experiments, however, are done at constant temperatures. in nature, mosquitoes are more likely to experience daily fluctuations in temperature. here we compare disseminated infection (leg i ...201829040730
the effect of insecticidal stress on reproductive output of susceptible and field strains of aedes aegypti (diptera: culicidae).the phenomenon of high egg reproduction when mortality risk rises is common in mosquitoes. however, the phenomenon may vary between insecticide susceptible and field-collected strains, due to the latter's decreased energy allocation in reproduction in the presence of insecticide resistance. in this study, we evaluated the effect of chlorpyrifos (cp) and temephos (tp) exposure on the oviposition and survival of aedes aegypti (linnaeus) (diptera: culicidae) using a susceptible strain (khsm) and tw ...201829040719
sublethal exposure to deltamethrin reduces the abilities of giant water bugs to prey upon aedes aegypti larvae.freshwater ecosystems provide environmental conditions for many arthropod species, including pests like mosquitoes and beneficial insects. giant water bugs, belostoma anurum (hemiptera: belostomatidae), are aquatic insects that provide biological control of mosquitoes and small vertebrates in freshwater environments. however, the application of insecticides aiming to control mosquitoes can lead to insecticide exposures of aquatic predators that can result in their death or significant reductions ...201829049958
detection and serotyping of dengue viruses in aedes aegypti and aedes albopictus (diptera: culicidae) collected in surabaya, indonesia from 2008 to 2015.aedes aegypti and aedes albopictus are the primary and secondary vectors, respectively, of dengue, the most important arboviral disease in the world. the aim of this study was to detect and serotype dengue viruses (denv) in the vectors ae. aegypti and ae. albopictus in surabaya, indonesia. between 2008 and 2015, 16,605 aedes mosquitoes were collected in 15 sub-districts of surabaya. ae. aegypti was dominant (90.9%), whereas few ae. albopictus were collected (9.1%). a total of 330 pools of adult ...201829093315
gold nanoparticles - against parasites and insect vectors.nanomaterials are currently considered for many biological, biomedical and environmental purposes, due to their outstanding physical and chemical properties. the synthesis of gold nanoparticles (au nps) is of high interest for research in parasitology and entomology, since these nanomaterials showed promising applications, ranging from detection techniques to drug development, against a rather wide range of parasites of public health relevance, as well as on insect vectors. here, i reviewed curr ...201829092797
the bacterium wolbachia exploits host innate immunity to establish a symbiotic relationship with the dengue vector mosquito aedes aegypti.a host's immune system plays a central role in shaping the composition of the microbiota and, in return, resident microbes influence immune responses. symbiotic associations of the maternally transmitted bacterium wolbachia occur with a wide range of arthropods. it is, however, absent from the dengue and zika vector mosquito aedes aegypti in nature. when wolbachia is artificially forced to form symbiosis with this new mosquito host, it boosts the basal immune response and enhances the mosquito's ...201829099491
influence of location and distance of biogents sentinel™ traps from human-occupied experimental huts on aedes aegypti recapture and entry into huts.a mark-release-recapture study was conducted to refine the "push-pull" strategy for controlling the dengue, chikungunya, and zika virus vector aedes aegypti in a peridomestic environment by determining optimal locations and distances from human-occupied experimental huts for placement of the "pull" component (biogents sentinel™ [bgs] traps) to maximize the capture of mosquitoes. the bgs traps were placed at portals of entry (windows or doors) or corners of the experimental huts and at varying di ...201831442172
habitat characteristics for immature stages of aedes aegypti in zanzibar city, tanzania.aedesaegypti is the main vector for dengue, chikungunya, yellow fever, zika, and other arboviruses of public health importance. the presence of ae. aegypti has never been systematically assessed in zanzibar, including its preferred larval habitats. in 2016 we conducted a cross-sectional entomological survey to describe the preferred larval habitats of ae. aegypti in zanzibar city, the main urban area of the zanzibar archipelago. the surveys for container habitats were conducted for a 17-wk perio ...201831442169
rapid immobilization of adult aedes aegypti caused by plant essential oils at sublethal concentrations.many synthetic insecticides cause immobilization in insect pests after they are exposed. this immobilization or knockdown is an important feature of intoxication that contributes to the abatement of pest insect populations, while preventing vectors of disease from biting and spreading pathogenic organisms to susceptible individuals. we have previously demonstrated that certain plant essential oils rapidly immobilize adult female mosquitoes that have been exposed via topical application. to furth ...201831442168
entomological investigation of aedes aegypti in neighborhoods with confirmed human arbovirus infection in puerto rico.the exotic arboviruses chikungunya (chikv) and zika (zikv) recently caused large outbreaks and continue to circulate in puerto rico, prompting entomological investigations at 9 locations with confirmed chikv- or zikv-infected human cases. adult mosquitoes were collected using the centers for disease control and prevention autocidal gravid ovitraps over a 14-day period at each site. mean female aedes aegypti captured per trap-week ranged from 13.47 per trap-week to 1.27 per trap-week. arbovirus-p ...201831442166
repellent activity of carrot seed essential oil and its pure compound, carotol, against mosquitoes.in our natural products screening program for mosquitoes, carrot seed essential oil showed high repellency. the gas chromatography (gc)/flame ionization detector and gc/mass spectrometry analysis of the essential oil revealed the presence of 47 compounds. carotol was more than 75% w/w, followed by muurolene (4.86%), (z)-β-farnesene (2.9%), and diepicedrene (1.1%). systematic bioassay-guided fractionation of the essential oil was performed to identify active repellent compounds. in both klun and ...201831442148
origin of aedes aegypti in clark county, nevada.aedesaegypti is the primary vector for serious diseases, including those caused by chikungunya, dengue, and zika viruses. in 2017, the southern nevada health district first detected this invasive species in clark county, nv, including in the city of las vegas. we analyzed ae. aegypti from the city of north las vegas to determine the likely source of the invasion. we genotyped a sample of ae. aegypti at 12 highly variable microsatellites and analyzed the data in reference to published data from 2 ...201831442147
molecular basis for arbovirus transmission by aedes aegypti mosquitoes.mosquitoes are considered the most important vectors for the transmission of pathogens to humans. aedes aegypti is a unique species, not only by its highly anthropophilic and peridomestic habits but also because it can transmit an important variety of pathogenic viruses. examples are dengue, yellow fever, chikungunya, zika, and mayaro viruses. after ingesting viremic blood, a wide range of mechanisms are activated in the mosquito to counteract viral infection. nevertheless, these arboviruses pos ...201831082816
wing geometry analysis of aedes aegypti (diptera, culicidae), a dengue virus vector, from multiple geographical locations of samut songkhram, thailand.dengue haemorrhagic fever (dhf) is a mosquito-borne disease and remains a major public health problem, especially in tropical and temperate countries. studying wing morphometric of aedes aegypti as a mosquito vector of dhf can help to better understand biological process of the mosquito adaptation to the environment. we aimed to study the geometric morphometric of ae. aegypti from multiple geographical areas.201830918904
effects of multiple transmission pathways on zika dynamics.although the zika virus is transmitted to humans primarily through the bite of infected female aedes aegypti mosquitoes, it can also be sexually and vertically transmitted within both populations. in this study, we develop a new mathematical model of the zika virus which incorporates sexual transmission in humans and mosquitos, vertical transmission in mosquitos, and mosquito to human transmission through bites. analysis of this deterministic model shows that the secondary transmission routes of ...201830839920
green synthesis of copper oxide nanoparticles and mosquito larvicidal activity against dengue, zika and chikungunya causing vector aedes aegypti.in the present study, high purity copper oxide nanoparticles (nps) were synthesised using tridax procumbens leaf extract. green syntheses of nano-mosquitocides rely on plant compounds as reducing and stabilising agents. copper oxide nps were characterised using x-ray diffraction (xrd) analysis, fourier transform infrared (ft-ir), field-emission scanning electron microscopy with energy dispersive spectroscopy, ultraviolet-visible spectrophotometry and fluorescence spectroscopy. xrd studies of the ...201830964011
[communication for arboviral disease prevention: tailoring paho initiatives to the cuban contextcomunicação para prevenção de arboviroses: adequação das iniciativas da opas ao contexto cubano].the purpose of this paper is to describe the opportunities and challenges of training processes in the coordination of regional and national communication initiatives for arboviral disease prevention, using the lessons learned in the cuban context as an example. in february 2016 and december 2017, professional staff from the pedro kourí institute of tropical medicine attended workshops for strengthening communication capacities for arboviral disease prevention in primary health care settings thr ...201831093174
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