Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| entrococcus faecalis elimination in root canals using silver nanoparticles, photodynamic therapy, diode laser, or laser-activated nanoparticles: an in vitro study. | the aim of this study was to compare the efficacy of silver nanoparticles (agnps), an 810-nm diode laser (dl), conventional photodynamic therapy (pdt) with the use of indocyanine green (icg) photosensitizer, and modified pdt with the use of agnps for the disinfection of root canals inoculated with enterococcus faecalis. | 2017 | 28027821 |
| crip1, a novel immune-related protein, activated by enterococcus faecalis in porcine gastrointestinal epithelial cells. | cysteine-rich intestinal protein 1 (crip1) is an important transcriptional regulation factor during the tumor development. although it was largely studied in the human or mouse, no report has provided functional evidence for it in the swine. to date, the real sequence of porcine crip1 (pocrip1) was also still unknown. in this study, clear characteristics for the pocrip1 were represented. a 552bp pocrip1 cdna was obtained from porcine brain tissue using real time reverse transcriptase pcr. the po ... | 2017 | 27836662 |
| antimicrobial influence of different root canal filling techniques in experimentally infected human root canals. | the aim of the present study was to evaluate the antimicrobial influence of different root canal filling techniques using gutta-percha and an epoxy resin-based sealer in experimentally infected root canals of extracted human teeth. | 2017 | 27834415 |
| intratubular disinfection with tri-antibiotic and calcium hydroxide pastes. | the aim of this study was to compare the in vitro intradentinal antimicrobial ability of the calcium hydroxide and tri-antibiotic pastes. | 2017 | 27866468 |
| effectiveness of final decontamination protocols against enterococcus faecalis and its influence on bond strength of filling material to root canal dentin. | the aim of this study was to evaluate the effectiveness of final decontamination protocols against enterococcus faecalis and their influence on bond strength of filling material to root canal dentin. | 2017 | 27866000 |
| triterpene glycosides from the aerial parts of gouania longipetala. | six previously undescribed triterpenoid saponins, gouaniaside i-vi, were isolated from the aerial parts of gouania longipetala hemsl. (rhamnaceae), in addition to four known triterpenes. the structure elucidation of these compounds was based on analyses of spectroscopic data including 1d- and 2d-nmr and hr-esi-ms techniques. the inhibitory activity of isolated compounds against promyelocytic leukemia hl60 and human erythromyeloblastoid leukemia k562 cell lines was evaluated and jujuboside i exhi ... | 2017 | 27865442 |
| quantification of the d-ala-d-lac-terminated peptidoglycan structure in vancomycin-resistant enterococcus faecalis using a combined solid-state nuclear magnetic resonance and mass spectrometry analysis. | induction of vancomycin resistance in vancomycin-resistant enterococci (vre) involves replacement of the d-ala-d-ala terminus of peptidoglycan (pg) stems with d-ala-d-lac, dramatically reducing the binding affinity of vancomycin for lipid ii. effects from vancomycin resistance induction in enterococcus faecalis (atcc 51299) were characterized using a combined solid-state nuclear magnetic resonance (nmr) and liquid chromatography-mass spectrometry (lc-ms) analysis. solid-state nmr directly measur ... | 2017 | 28040891 |
| a comparison between effect of photodynamic therapy by led and calcium hydroxide therapy for root canal disinfection against enterococcus faecalis: a randomized controlled trial. | insufficient root canal disinfection is one of the main reasons for persistent periapical pathology. photodynamic therapy (pdt) has been proven effective in disinfecting infected root canals. the aim of this study was to evaluate the antimicrobial effect of photo activated disinfection (pad) when using toluidine blue as photosensitizer and a led lamp after the conventional treatment, and comparing it with calcium hydroxide therapy in vivo. | 2017 | 28040502 |
| novel lnu(g) gene conferring resistance to lincomycin by nucleotidylation, located on tn6260 from enterococcus faecalis e531. | to identify a novel putative lincosamide resistance gene determinant in a swine enterococcus faecalis e531 exhibiting a lincosamide resistance/macrolide susceptibility (l r m s ) phenotype and to determine its location and genetic environment. | 2017 | 28039271 |
| synthesis of peptidoglycan fragments from enterococcus faecalis with fmoc-strategy for glycan elongation. | peptidoglycan (pgn) is an essential structural component of the bacterial cell wall conferring cell shape, which can be recognized by host-recognition proteins and receptors as well as bacterial surface proteins. in this work, the pgn partial structures from enterococcus faecalis that contain a tetrasaccharide and an octasaccharide with a unique heptapeptide were synthesized via an fmoc-strategy for elongation of the glycan chains. namely, a 4'-o-fmoc-protected disaccharide was utilized as the k ... | 2017 | 27868361 |
| randomized clinical trial of preoperative skin antisepsis with chlorhexidine gluconate or povidone-iodine. | skin antiseptic agents are used to prevent surgical-site infection (ssi); few trials have reported the superiority of any specific agent in clean-contaminated abdominal surgery. this rct was designed to compare the effectiveness of chlorhexidine gluconate and povidone-iodine. | 2017 | 27879993 |
| pharmacodynamics of ceftaroline plus ampicillin against enterococcus faecalis in an in vitro pharmacokinetic/pharmacodynamic model of simulated endocardial vegetations. | the combination of ampicillin plus ceftaroline has been suggested to be more reliably synergistic against enterococcus faecalis than ampicillin plus ceftriaxone using time-kill methods. the purpose of this study was to determine if this trend persists in a two-compartment model of simulated endocardial vegetations (sev) using clinically relevant pharmacokinetic exposures of these antimicrobials. three clinically derived e. faecalis strains were included in the study. the mics of study antimicrob ... | 2017 | 28096164 |
| ofloxacin loaded gellan/pva nanofibers - synthesis, characterization and evaluation of their gastroretentive/mucoadhesive drug delivery potential. | the purpose of this investigation is to formulate a gastroretentive sustained drug release system for ofloxacin to improve its retention time, pharmacological activity, bioavailability and therapeutic efficacy in the stomach. ofloxacin loaded gellan/poly vinyl alcohol (pva) nanofibers were fabricated using a simple and versatile electrospinning technique. the fabricated nanofibers were evaluated for percent drug encapsulation efficiency and in vitro drug release in simulated gastric medium (ph1. ... | 2017 | 27987752 |
| culture methods impact recovery of antibiotic-resistant enterococci including enterococcus cecorum from pre- and postharvest chicken. | pathogenic strains of enterococcus cecorum (ec) expressing multidrug resistance have emerged. in national antimicrobial resistance monitoring system (narms) data, ec is rarely recovered from chickens. two narms methodologies (fda and usda) were compared with standard culture (sc) techniques for recovery of ec. narms methods failed to detect ec in 58 caecal samples, 20 chicken breast or six whole broiler samples. ec was recovered from 1 of 38 (2·6%) and 2 of 38 (5·2%) preharvest spinal lesions (u ... | 2017 | 27987523 |
| integrated multivariate analysis with nondetects for the development of human sewage source-tracking tools using bacteriophages of enterococcus faecalis. | we developed sewage-specific microbial source tracking (mst) tools using enterococci bacteriophages and evaluated their performance with univariate and multivariate analyses involving data below detection limits. newly isolated enterococci faecalis bacterial strains aim06 (dsm100702) and sr14 (dsm100701) demonstrated 100% specificity and 90% sensitivity to human sewage without detecting 68 animal manure pooled samples of cats, chickens, cows, dogs, ducks, pigs, and pigeons. aim06 and sr14 bacter ... | 2017 | 27983829 |
| rapid assessment of resistance to antibiotic inhibitors of protein synthesis in the gram-positive pathogens, enterococcus faecalis and streptococcus pneumoniae, based on evaluation of the lytic response. | a novel assay for rapid determination of resistance to antibiotic inhibitors of protein synthesis was developed for the gram-positive pathogens, enterococcus faecalis and streptococcus pneumoniae. to this purpose, a lytic response was obtained by a brief incubation with lysozyme or a mixture of lysozyme, triton x-100, and edta for e. faecalis (n = 82) and s. pneumoniae (n = 51), respectively. lysis was quantified by visualizing the released nucleoids. antibiotic-susceptible bacteria treated with ... | 2017 | 27383851 |
| comparative evaluation of antimicrobial effect of herbal root canal irrigants (morinda citrifolia, azadirachta indica, aloe vera) with sodium hypochlorite: an in vitro study. | successful root canal treatment involves the complete elimination of microorganism from the root canal and the three-dimensional obturation of the canal space. enterococcus faecalis is the most commonly found bacteria in failed root canal. chemical irrigation of canals along with biomechanical preparation helps in the elimination of microorganisms. the present study was aimed to evaluate the antimicrobial effect of herbal root canal irrigants (morinda citrifolia, azadirachta indica extract, aloe ... | 2017 | 27382533 |
| probiotic enterococcus faecalis symbioflor(®) down regulates virulence genes of ehec in vitro and decrease pathogenicity in a caenorhabditis elegans model. | enterohemorrhagic e. coli o157:h7 (ehec) shorten the lifespan of caenorhabditis elegans compared to avirulent bacteria. co-feeding ehec with enterococcus faecalis symbioflor(®) significantly increased the worms' lifespan. the transcriptome of ehec grown in vitro with or without symbioflor(®) was analyzed using rna-seq. the analysis revealed downregulation of several virulence-associated genes in the presence of symbioflor(®), including virulence key genes (e.g., lee, flagellum, quorum-sensing). ... | 2017 | 27655246 |
| to defend or not to defend: that's the question. | enterococcus faecalis is an opportunistic pathogen and is one of the leading causes of nosocomial infections. e. faecalis harbors a number of antibiotic resistance genes, and most of these are present on mobile genetic elements (mges) that can be disseminated within the species, as well as to other members of the human microflora. in an article by price and colleagues [v. j. price et al., msphere 1(3):e00064-16, 2016, http://dx.doi.org/10.1128/msphere.00064-16], the authors demonstrated how e. f ... | 2017 | 27306929 |
| synthesis and biological evaluation of lipophilic teicoplanin pseudoaglycon derivatives containing a substituted triazole function. | a series of lipophilic teicoplanin pseudoaglycon derivatives, including alkyl-, aryl-, calixarene- and protected sugar-containing conjugates, were prepared using azide-alkyne click chemistry. out of the conditions applied, the cuso4-ascorbate reagent system proved to be more efficient than the cu(i)i-et3n-mediated reaction. some of the new compounds have high in vitro activity against glycopeptide-resistant gram-positive bacteria, including vana-positive enterococcus faecalis. a few of them also ... | 2017 | 27353163 |
| characteristics of vancomycin-resistant enterococcus strains in the west balkans: a first report. | vancomycin-resistant enterococci are among the major causes of nosocomial infections and represent a growing problem in many european countries. among the most common enterococcal isolates, enterococcus faecium is considered to be the reservoir of vana and vanb-mediated resistance to glycopeptides. enterococci with vana-mediated resistance can transfer resistance genes to other enterococci and gram-positive bacteria. hence, monitoring and surveillance of vancomycin-resistant enterococci (vres) a ... | 2017 | 27351410 |
| multi-drug-resistant enterococcus faecalis among egyptian patients with urinary tract infection. | the prevalence of enterococcus faecalis (e. faecalis) infections among egyptians with urinary tract infection (uti), their antimicrobial susceptibility and mechanisms of resistance are under investigated. in this study, 300 urine samples were collected from uti patients to identify e. faecalis. antimicrobial susceptibility to 18 antimicrobial agents was tested. the presence of aac(6)-ie-aph(2)ia, erm(b) and mef(a/e) genes was examined by pcr. fifty-seven (19%) isolates were identified as e. faec ... | 2017 | 27351108 |
| in vitro comparison of antibacterial properties of bioceramic-based sealer, resin-based sealer and zinc oxide eugenol based sealer and two mineral trioxide aggregates. | our goal of the study was to evaluate the antibacterial properties of endodontic sealers against the e. faecalis. | 2017 | 27403055 |
| crispr-cas and restriction-modification act additively against conjugative antibiotic resistance plasmid transfer in enterococcus faecalis. | enterococcus faecalis is an opportunistic pathogen and a leading cause of nosocomial infections. conjugative pheromone-responsive plasmids are narrow-host-range mobile genetic elements (mges) that are rapid disseminators of antibiotic resistance in the faecalis species. clustered regularly interspaced short palindromic repeat (crispr)-cas and restriction-modification confer acquired and innate immunity, respectively, against mge acquisition in bacteria. most multidrug-resistant e. faecalis isola ... | 2017 | 27303749 |
| lactobacillus helveticus lafti l10 supplementation modulates mucosal and humoral immunity in elite athletes: a randomized, double-blind, placebo-controlled trial. | michalickova, dm, kostic-vucicevic, mm, vukasinovic-vesic, md, stojmenovic, tb, dikic, nv, andjelkovic, ms, djordjevic, bi, tanaskovic, bp, and minic, rd. lactobacillus helveticus lafti l10 supplementation modulates mucosal and humoral immunity in elite athletes: a randomized, double-blind, placebo-controlled trial. j strength cond res 31(1): 62-70, 2017-to test the influence of probiotic supplementation on humoral immune response, a double-blind, placebo-controlled trial was conducted. thirty a ... | 2017 | 27100317 |
| antimicrobial effect of three disinfecting agents on resilon cones and their effect on surface topography: an in vitro study. | the objective of this study was to evaluate the effectiveness of 5.25% sodium hypochlorite (naocl), 2% chlorhexidine (chx), and 2% peracetic acid (paa) in disinfecting resilon cones and to evaluate topographical changes microscopically under scanning electron microscope (sem) after rapid chemical disinfection. | 2017 | 27099418 |
| pretreatment with a heat-killed probiotic modulates monocyte chemoattractant protein-1 and reduces the pathogenicity of influenza and enterovirus 71 infections. | it has been proposed that inactivated probiotics may modulate the host immune system and contribute to mitigation of viral infections. this study demonstrated that administration of heat-killed enterococcus faecalis, a widely used probiotic, can protect host animals against viral infections. the influenza-mediated morbidity and lung inflammation in e. faecalis-treated mice decreased significantly compared with those of the control mice. furthermore, we found that the protection is associated wit ... | 2017 | 27072606 |
| effect of laser-activated irrigation on biofilms in artificial root canals. | to evaluate the antimicrobial effect of laser-activated irrigation (lai) on biofilms formed in simulated root canals. | 2017 | 27014832 |
| molecular characterization of virulence genes in vancomycin-resistant and vancomycin-sensitive enterococci. | the aim of this study was to find out the correlation between presence of virulence (gelatinase [gel e], enterococcal surface protein [esp], cytolysin a [cyl a], hyaluronidase [hyl], and aggregation substance [asa1]) and vancomycin-resistant genes (van a and van b) in enterococci, with their phenotypic expression. | 2017 | 27013840 |
| community acquired enterococcal urinary tract infections and antibiotic resistance profile in north india. | urinary tract infections (utis) remain a major problem both in hospitalized and outdoor patients. multidrug-resistant enterococci are emerging as a major nosocomial pathogen with increasing frequency. however, the incidence of community-acquired enterococcal infections and species prevalent in india is not thoroughly investigated. | 2017 | 27013814 |
| light and temperature effects on bioactivity in diatoms. | isolates of five pelagic north atlantic marine diatoms (bacillariophyceae): attheya longicornis, chaetoceros socialis, chaetoceros furcellatus, skeletonema marinoi and porosira glacialis were cultivated in large photobioreactors at two light and two temperature regimes to test if this affected bioactivity. we screened for bioactivity in assays representing five different therapeutic areas: diabetes ii (ptp1b), cancer (melanoma cells, a2058), anti-oxidants (frap), immunomodulation (tnfa) and anti ... | 2017 | 27057087 |
| the prevalence of aminoglycoside-modifying enzyme and virulence genes among enterococci with high-level aminoglycoside resistance in inner mongolia, china. | this study highlights the prevalence of aminoglycoside-modifying enzyme genes and virulence determinants among clinical enterococci with high-level aminoglycoside resistance in inner mongolia, china. screening for high-level aminoglycoside resistance against 117 enterococcal clinical isolates was performed using the agar-screening method. out of the 117 enterococcal isolates, 46 were selected for further detection and determination of the distribution of aminoglycoside-modifying enzyme-encoding ... | 2017 | 27268115 |
| detection of vancomycin resistance in enterococcus species isolated from clinical samples and feces of colonized patients by phenotypic and genotypic methods. | the aim of this study was to find out the clinical correlation between the presence of vancomycin-resistant genes (van a and van b) and their expression as detected by phenotypic tests in colonized patients and in clinical isolates. | 2017 | 27166038 |
| the effect of berberine hydrochloride on enterococcus faecalis biofilm formation and dispersion in vitro. | enterococcus faecalis (e. faecalis) is one of the major causes of biofilm infections. berberine hydrochloride (bbh) has diverse pharmacological effects; however, the effects and mechanisms of bbh on e. faecalis biofilm formation and dispersion have not been reported. in this study, 99 clinical isolates from the urine samples of patients with urinary tract infections (utis) were collected and identified. ten strains of e. faecalis with biofilm formation ability were studied. bbh inhibited e. faec ... | 2017 | 27242142 |
| effectiveness of n-acetyl cysteine, 2% chlorhexidine, and their combination as intracanal medicaments on enterococcus faecalis biofilm. | the purpose of this study was to evaluate the antibacterial efficacies of 2% chlorhexidine (chx), n-acetyl cysteine (nac) and assess their synergistic or antagonist action as intracanal medicament. | 2017 | 26957787 |
| comparative antimicrobial efficacy of herbal alternatives (emblica officinalis, psidium guajava), mtad, and 2.5% sodium hypochlorite against enterococcus faecalis: an in vitro study. | the objective of this study was to evaluate the antimicrobial efficacy of herbal alternatives (emblica officinalis, psidium guajava), biopure mtad, and 2.5% sodium hypochlorite against enterococcus faecalis. | 2017 | 26937369 |
| in vitro antimicrobial evaluation of toothpastes with natural compounds. | this in vitro study evaluated the antimicrobial effects of commercial toothpastes containing natural compounds. | 2017 | 26929699 |
| enhancing the antibacterial activity of the gold standard intracanal medicament with incorporation of silver zeolite: an in vitro study. | enterococcus faecalis is a persistent organism that plays a major role in the etiology of persistent periradicular lesions after root canal treatment has been associated with different forms of periradicular disease including primary endodontic infections and persistent infections. the present study compares the antibacterial activities of calcium hydroxide, calcium hydroxide mixed with silver zeolite, and calcium hydroxide mixed with 2% chlorhexidine against e. faecalis using direct contact tes ... | 2017 | 27011937 |
| a new mechanism of post-transfer editing by aminoacyl-trna synthetases: catalysis of hydrolytic reaction by bacterial-type prolyl-trna synthetase. | aminoacyl trna synthetases are enzymes that specifically attach amino acids to cognate trnas for use in the ribosomal stage of translation. for many aminoacyl trna synthetases, the required level of amino acid specificity is achieved either by specific hydrolysis of misactivated aminoacyl-adenylate intermediate (pre-transfer editing) or by hydrolysis of the mischarged aminoacyl-trna (post-transfer editing). to investigate the mechanism of post-transfer editing of alanine by prolyl-trna synthetas ... | 2017 | 26886480 |
| efficacy of calcium oxide and calcium hydroxide nanoparticles on the elimination of enterococcus faecalis in human root dentin. | the objective of this study was to compare the antibacterial effect of calcium oxide nanoparticles (conps) and calcium hydroxide nanoparticles (chnps) against enterococcus faecalis in a dentinal block model. | 2017 | 27129586 |
| influence of temperature on the antibacterial activity of sodium hypochlorite. | the aim of this study was to compare the antimicrobial activity of 5.25% naocl, hypoclean and chlor-xtra at 20 °c and 45 °c in bovine root dentin. one-hundred-and-seventy dentin tubes prepared from bovine maxillary incisors were infected for 21 days with enterococcus faecalis. the specimens were divided into the following groups: 1. 5.25% naocl 20 °c; 2. hypoclean 20 °c; 3. chlor-xtra 20 °c; 4. 5.25% % naocl 45 °c; 5. hypoclean 45 °c; 6. chlor-xtra 45 °c; 7. positive control; 8. negative control ... | 2017 | 27007342 |
| 2-hydroxyisocaproic acid is bactericidal in human dental root canals ex vivo. | to compare the activity of 2-hydroxyisocaproic acid (hica), calcium hydroxide (ca(oh)2 ) and chlorhexidine digluconate (chg) against enterococcus faecalis t-75359 (root canal isolate) in the root canals of extracted human teeth. | 2017 | 27004733 |
| antimicrobial effect of an oxazolidinone, lantibiotic and calcium hydroxide against enterococcus faecalis biofilm: an in vitro study. | the aim was to evaluate and compare the antimicrobial efficacy of an oxazolidinone (linezolid [lz]), lantibiotic (nisin), and calcium hydroxide against enterococcus faecalis biofilm formed on tooth substrate after 2 and 7 days. | 2017 | 26752879 |
| antimicrobial efficacy of different root canal sealers by using real-time polymerase chain reaction: an ex vivo study. | root canal sealers help to minimize leakage, provides antimicrobial activity to reduce the possibility of residual bacteria, and to resolve periapical lesion. | 2017 | 26752843 |
| quantitative evaluation of apical extrusion of intracanal bacteria using rotary protaper, k3xf, twisted and hand k-file system: an ex vivo study. | the aim of this study was to evaluate the number of intracanal bacteria extruded apically during root canal preparation using rotary protaper, k3xf, twisted, and hand k-file system. | 2017 | 26481889 |
| effect of passive ultrasonic irrigation on enterococcus faecalis from root canals: an ex vivo study. | endodontic irrigation aims to clean and disinfect the root canal system. passive ultrasonic irrigation (pui) is based on the use of an ultrasound-activated instrument into the root canal filled with irrigant. the aim of this study was to evaluate, ex vivo, the effectiveness of pui in eliminating enterococcus faecalis from root canals. seventy-five extracted human single-root teeth were used. after root canal preparation, specimens were inoculated with e. faecalis and incubated at 37 °c for 21 da ... | 2017 | 26312969 |
| biofilm forming capacity of enterococcus faecalis on gutta-percha points treated with four disinfectants using confocal scanning laser microscope: an in vitro study. | the aim of this study was to evaluate and compare the in vitro biofilm forming capacity of enterococcus faecalis on gutta-percha points disinfected with four disinfectants. | 2017 | 26288622 |
| antibiotic resistance pattern of enterococci isolates from nosocomial infections in a tertiary care hospital in eastern india. | resistance to commonly used antibiotics by enterococci causing nosocomial infections is of concern, which necessitates judicious, responsible and evidence-based use of antibiotics. the present study was conducted to review the prevalence and identify therapeutic options for nosocomial enterococcal infections in our tertiary care hospital. | 2017 | 26283837 |
| phytochemical screening, antioxidant and antibacterial activities of extracts prepared from different tissues of schinus terebinthifolius raddi that occurs in the coast of bahia, brazil. | schinus terebinthifolius is widely used in traditional medicine by brazilian quilombola and indigenous communities for treatment of several diseases. extracts from different tissues are being used to produce creams to treat cervicitis and cervicovaginitis. however, most studies are limited to the assessment of the essential oils and extracts obtained from the leaves. | 2017 | 26246739 |
| an assessment of antibacterial activity of three pulp capping materials on enterococcus faecalis by a direct contact test: an in vitro study. | the aim of this in vitro study was to evaluate antimicrobial activities of three different pulp capping materials; biodentine, mineral trioxide aggregate (mta) angelus, and dycal against enterococcus faecalis and their durability with time. | 2017 | 26038657 |
| physicochemical characterization of water-soluble chitosan derivatives with singlet oxygen quenching and antibacterial capabilities. | new water-soluble chitosan derivatives (wsch) were obtained by maillard reaction (mr) between glucosamine (ga) with both low and medium molecular weight chitosans (ch). the wsch showed larger solubility than the respective ch, while their deacetylation degree (dd) decreased by approximately 12%. infrared spectroscopy experiments of wsch confirmed the formation of imine bonds after mr with intensified pyranose structure, and sugar molecules as polymer branches. however, a 6-times reduction of the ... | 2017 | 28396271 |
| safety evaluation of enterocin producer enterococcus sp. strains isolated from traditional turkish cheeses. | the purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in enterococcus spp. isolated from different cheeses and also investigate some of their virulence factors. enterococcus strains were isolated from 33 different cheeses. enterococcus faecium (6 strains) and enterococcus faecalis (5 strains) enterocin-producing strains were identified by 16s rdna analyses. structural genes enta, entb, entp and entx were detected in some isolates. mu ... | 2017 | 28735309 |
| isolation and characterization of lactic acid bacteria from traditional pickles of himachal pradesh, india. | a total of 15 isolates of lactic acid bacteria (lab) were isolated from traditional pickles of rural and urban areas of himachal pradesh. these isolates were identified as enterococcus faecalis (7), lactobacillus plantarum (3), pediococcus pentosaceus (2), leuconostoc mesenteroides (1), lactococcus lactis (1) and enterococcus sp. (1) on the basis of morphological, biochemical and 16s rdna sequence analysis. all the labs produced exopolysaccharides, have antibiotic susceptibility, and possess act ... | 2017 | 28720951 |
| inactivation of bacterial biofilms by visible light-activated unmodified zno nanorods. | various zinc oxide (zno) nanostructures are widely used for photocatalytic antibacterial applications. since zno possess a wide bandgap, it is believed that only uv light may efficiently assist bacterial inactivation and diverse crystal lattice modifications should be applied in order to narrow the bandgap for efficient visible light absorption. in this work we show that even unmodified zno nanorods grown by aqueous chemical growth technique were found to possess intrinsic defects which can be a ... | 2017 | 28628486 |
| characterization of high exopolysaccharide-producing lactobacillus strains isolated from mustard pickles for potential probiotic applications. | the aim of this study was to characterize high exopolysaccharide (eps)-producing lactic acid bacteria (lab) isolated from mustard pickles in taiwan for potential probiotic applications. among 39 collected lab strains, four most productive eps-producing strains were selected for further analysis. comparative analyses of 16s rdna genes rpoa and phes sequences demonstrated that these strains were members of lactobacillus plantarum-group (lpg). ncd 2, nld 4, slc 13, and nld 16 showed survival rates ... | 2017 | 28617525 |
| seven new lignan glycosides from the branches of alangium kurzii craib var. laxifolium. | seven new lignan glycosides (1-3, 8-10, and 14) and 17 known compounds were isolated from the branches of alangium kurzii craib var. laxifolium. their structures were established by spectroscopic analysis and circular dichroism (cd) and x-ray analysis. the isolated compounds were evaluated for their antibacterial activities against staphylococcus aureus ci1011, streptococcus suis ci1608, salmonella gallinarum ci0912, enterococcus faecalis ci1304, aeromonas hydrophila ci1008, escherichia coli ci1 ... | 2017 | 28733150 |
| do quaternary ammonium monomers induce drug resistance in cariogenic, endodontic and periodontal bacterial species? | antibacterial monomers were developed to combat oral biofilm acids and caries; however, little is known on whether quaternary ammonium monomers (qams) would induce drug resistance in oral bacteria. the objective of this study was to investigate the effects of new antimicrobial monomers dimethylaminohexadecyl methacrylate (dmahdm) and dimethylaminododecyl methacrylate (dmaddm) on the induction of drug resistance in eight species of cariogenic, endodontic and periodontal bacteria for the first tim ... | 2017 | 28755761 |
| validity of somatic cell count as indicator of pathogen-specific intramammary infections. | the objective of this study was to determine whether somatic cell count (scc) was an effective test, with a sensitivity exceeding 85%, to determine species-specific bacterial infections. in addition, the relation between the scc and various udder pathogen groups was investigated. scc thresholds of greater than 200 000 cells/ml were used in quarter and greater than 150 000 cells/ml in composite milk samples. a retrospective study was conducted on a data set for 89 635 quarter and 345 467 composit ... | 2017 | 28470079 |
| the curious case of achromobacter eurydice, a gram-variable pleomorphic bacterium associated with european foulbrood disease in honeybees. | honeybees are prone to parasite and pathogen infestations/infections due to their social colony life. bacterial pathogens in particular lead to destructive infections of the brood. european foulbrood is caused by the bacterium melissococcus plutonius in combination with several other gram-positive bacteria (achromobacter eurydice, bacillus pumilus, brevibacillus laterosporus, enterococcus faecalis, paenibacillus alvei, paenibacillus dendritiformis) involved as secondary invaders following the in ... | 2017 | 28634639 |
| chemical constituents of helichrysum italicum (roth) g. don essential oil and their antimicrobial activity against gram-positive and gram-negative bacteria, filamentous fungi and candida albicans. | the aerial parts of helichrysum italicum (roth) g. don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. the essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). the most o ... | 2017 | 28725151 |
| 1,1ꞌ-carbonyldiimidazole (cdi) mediated facile synthesis, structural characterization, antimicrobial activity and in-silico studies of coumarin-3-carboxamide derivatives. | despite of the availability of variety of antibacterial agents, re-emergance of pathogenic bacteria is still a serious medical concern. so, identification of new, safer, and selective antibacterial agents is the key interest in the medicinal chemistry research. | 2017 | 28641525 |
| cytotoxic and antimicrobial activities of cantharellus cibarius fr. (cantarellaceae). | antibacterial and cytotoxic activities of cyclohexane, dichloromethane, methanol, and aqueous extracts of cantharellus cibarius were tested. broth microdilution assay was performed against 10 bacterial strains (staphylococcus aureus, s. epidermidis, micrococcus luteus, bacillus subtilis, enterococcus feacalis, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella abony), with emphasis on helicobacter pylori. methanol extract was the most active against h. pylori strains wit ... | 2017 | 28613963 |
| antibacterial and anticancer activities of acetone extracts from in vitro cultured lichen-forming fungi. | lichens that were used in traditional medicine for ages produce numerous secondary metabolites, however our knowledge about biological activities of substances secreted by separated bionts is scarce. the main objectives of this study were to isolate and find optimal conditions for the growth of mycelia from three common lichen-forming fungi, i.e. caloplaca pusilla, protoparmeliopsis muralis and xanthoria parietina and to evaluate antibacterial and antiproliferative activities of their acetone ex ... | 2017 | 28592323 |
| ex vivo microbial leakage after using different final irrigation regimens with chlorhexidine. | to assess the influence of final irrigation protocols with chlorhexidine in the coronal leakage of enterococcus faecalis in filled root canals. | 2017 | 23559116 |
| antibacterial effect of azadirachta indica (neem) or curcuma longa (turmeric) against enterococcus faecalis compared with that of 5% sodium hypochlorite or 2% chlorhexidine in vitro. | the purpose of this study was to compare the antibacterial properties of azadirachta indica (neem) or curcuma longa (turmeric) against enterococcus faecalis with those of 5% sodium hypochlorite or 2% chlorhexidine as root canal irrigants in vitro. the activity of neem, chlorhexidine, sodium hypochlorite, or turmeric against e. faecalis was measured on agar plates using the agar diffusion method. the tube dilution method was used to determine the minimum inhibitory concentration (mic) and minimum ... | 2017 | 28724858 |
| biofilm formation in enterococci: genotype-phenotype correlations and inhibition by vancomycin. | enterococci are nosocomial pathogens that can form biofilms, which contribute to their virulence and antibiotic resistance. although many genes involved in biofilm formation have been defined, their distribution among enterococci has not been comprehensively studied on a genome scale, and their diagnostic ability to predict biofilm phenotypes is not fully established. here, we assessed the biofilm-forming ability of 90 enterococcal clinical isolates. major patterns of virulence gene distribution ... | 2017 | 28720810 |
| virulence genes in biofilm producer enterococcus faecalis isolates from root canal infections. | enterococcus faecalis is occurring in opportunistic infections involving the oral cavity. this study aimed to evaluate the presence of e. faecalis virulence genes in dental root canal isolates recovered from advanced chronic periodontitis patients. one hundred e. faecalis isolated from dental root canal during july 2015 to oct 2016. after analysis of biofilm formation by the semi-quantitative determination in 96-well flat bottom polystyrene plates, the presence of asa, esp, efaa, ace, ebpr, gel ... | 2017 | 28719346 |
| diversity of antagonistic bacteria isolated from medicinal plant peganum harmala l. | the antimicrobial activity of plant extract of peganum harmala, a medicinal plant has been studied already. however, knowledge about bacterial diversity associated with different parts of host plant antagonistic to different human pathogenic bacteria is limited. in this study, bacteria were isolated from root, leaf and fruit of plant. among 188 bacterial isolates isolated from different parts of the plant only 24 were found to be active against different pathogenic bacteria i.e. escherichia coli ... | 2017 | 28855824 |
| effect of conventional irrigation and photoactivated disinfection on enterococcus faecalis in root canals: an in vitro study. | a study was done to evaluate the antimicrobial efficacy of sodium hypochlorite (naocl) and photoactivated disinfection (pad) on enterococcus faecalis. | 2017 | 28855761 |
| enhancing antibacterial effect of sodium hypochlorite by low electric current-assisted sonic agitation. | this research focused on the effects of low electric current (μe)-assisted sonic agitation of sodium hypochlorite on enterococcus faecalis infected human root dentin. | 2017 | 28854274 |
| screening of oxalate degrading lactic acid bacteria of food origin. | a screening for oxalate degrading abilities was initially carried on within lactic acid bacteria cultures of different food origin. seventy-nine strains were drop-inoculated onto mrs agar plates containing calcium oxalate. by comparing colonies diameters, 31 strains were used to inoculate, in parallel, mrs and mrs modified by sodium oxalate addition. differences in the strains' growth were assessed by colony forming unit counts. for two strains, the growth in oxalate enriched medium was signific ... | 2017 | 28713789 |
| genetic characterisation confirms sporadic occurrence of vancomycin-resistant enterococcus faecalis in copenhagen, denmark. | 2017 | 28709988 | |
| molecular determinants of the matrix thickening of a dual-species pseudomonas aeruginosa and enterococcus faecalis biofilm. | biofilms are microbial communities that inhabit various surfaces and are surrounded by extracellular matrices (ecms). clinical microbiologists have shown that the majority of chronic infections are caused by biofilms, after introduction of the first biofilm infection model by j.w. costerton and his colleagues (1). however, treatments for chronic biofilm infections are still limited to surgical removal of the infected sites. pseudomonas aeruginosa and enterococcus faecalis are two frequently iden ... | 2017 | 28842537 |
| how proteases from enterococcus faecalis contribute to its resistance to short α-helical antimicrobial peptides. | hyl-20 (gilsslwkklkkiiak-nh2) is an analogue of a natural antimicrobial peptide (amp) previously isolated from the venom of wild bee. we examined its antimicrobial activity against three strains of enterococcus faecalis while focusing on its susceptibility to proteolytic degradation by two known proteases-gelatinase (gele) and serine protease (spre)-which are secreted by these bacterial strains. we found that hyl-20 was primarily deamidated at its c-terminal which made the peptide susceptible to ... | 2017 | 28830077 |
| small alarmone synthetases as novel bacterial rna-binding proteins. | the alarmone nucleotides guanosine pentaphosphate (pppgpp) and tetraphosphate (ppgpp), collectively referred to as (p)ppgpp, are key regulators of bacterial growth, stress adaptation, antibiotic tolerance and pathogenicity. we have recently shown that the small alarmone synthetase (sas) relq from the gram-positive pathogen enterococcus faecalis has an rna-binding activity (beljantseva et al. 2017). relq's activities as an enzyme and as a rna-binding protein are mutually incompatible: binding of ... | 2017 | 28820325 |
| [elimination of entercoccus faecalis with different disinfection methods in root canals in vitro]. | to compare the elimination effect against e.faecalis in root canals with different methods. | 2017 | 28815239 |
| short-term alcohol abstinence improves antibacterial defenses of chronic alcohol-consuming mice against gut bacteria-associated sepsis caused by enterococcus faecalis oral infection. | the effects of short-term alcohol abstinence on host antibacterial resistance against enterococcus faecalis oral infection was investigated in chronic alcohol-consuming mice [mice with 0.1 g/day of 20% ethanol consumption for 12 or 16 weeks (cac-mice)]. these mice were highly susceptible to the infection; however, after 7 days of alcohol abstinence (aacac-mice), their antibacterial resistances were completely restored to the normal mouse level. normal mice inoculated with cac-mouse hepatic macro ... | 2017 | 28708971 |
| virulence and antibiotic resistance of enterococci isolated from healthy breastfed infants. | pathogenic ability has been extensively studied in clinical enterococci, but to a lesser extent in community-derived ones. most studies to date in enterococci from healthy infants have been focused on enterococcus faecalis, despite the growing concern about nosocomial infections caused by e. faecium. in this work, we studied the antibiotic resistance and virulence determinants of 26 e. faecalis and 15 e. faecium intestinal isolates from spanish healthy breastfed infants. overall, commensal enter ... | 2017 | 28708453 |
| growth- and stress-induced pasta kinase phosphorylation in enterococcus faecalis. | transmembrane ser/thr kinases containing extracellular pasta domains are ubiquitous among actinobacteria and firmicutes. such pasta kinases regulate critical processes including antibiotic resistance, cell division, toxin production, virulence, and are essential for viability in certain organisms. based on in vitro studies with purified extra- and intracellular fragments of pasta kinases, a model for signaling has been proposed, in which the extracellular pasta domains bind as-yet-undefined liga ... | 2017 | 28808126 |
| media from macrophages co-incubated with enterococcus faecalis induces epithelial cell monolayer reassembly and altered cell morphology. | signal exchange between intestinal epithelial cells, microbes and local immune cells is an important mechanism of intestinal homeostasis. given that intestinal macrophages are in close proximity to both the intestinal epithelium and the microbiota, their pathologic interactions may result in epithelial damage. the present study demonstrates that co-incubation of murine macrophages with e. faecalis strains producing gelatinase (gele) and serine protease (spre) leads to resultant condition media ( ... | 2017 | 28793333 |
| anti-enterococcal activities of pentacyclic triterpenes. | asiatic (aa) and ursolic (ua) acids are widely studied phytochemicals, but their antimicrobial properties are still poorly understood. therefore our research has focused on their activity against uropathogenic enterococcus faecalis strains. | 2017 | 28791824 |
| characterization of the tertiary structure of the peptidoglycan of enterococcus faecalis. | solid-state nmr spectra of whole cells and isolated cell walls of enterococcus faecalis grown in media containing combinations of (13)c and (15)n specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan. the compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross- ... | 2017 | 28784459 |
| coupling metagenomics with cultivation to select host-specific probiotic microorganisms for subtropical aquaculture. | to demonstrate a non-empirical workflow to select host-specific probiotics for aquaculture industry. | 2017 | 28783248 |
| molecular identification of multiple antibiotic resistant fish pathogenic enterococcus faecalis and their control by medicinal herbs. | the opportunistic fish pathogen, enterococcus faecalis has been reported to cause mass mortality in several fish species in different countries. the objectives of this study were to (i) identify e. faecalis from the diseased fishes through molecular techniques; (ii) assess the antibiotic susceptibility profile of e. faecalis isolates; and (iii) control disease in tilapia fish by treatment with medicinal plant extracts. a total of 48 isolates were phenotypically identified as enterococcus species ... | 2017 | 28623336 |
| bactericidal effect of er:yag laser-activated sodium hypochlorite irrigation against biofilms of enterococcus faecalis isolate from canal of root-filled teeth with periapical lesions. | this study was to evaluate the bactericidal effect of er:yag laser-activated sodium hypochlorite irrigation (er:yag + naocl) on biofilms of enterococcus faecalis clinical isolate. | 2017 | 28622484 |
| design, synthesis, and evaluation of novel tyrosine-based dna gyrase b inhibitors. | the discovery and synthesis of new tyrosine-based inhibitors of dna gyrase b (gyrb), which target its atpase subunit, is reported. twenty-four compounds were synthesized and evaluated for activity against dna gyrase and dna topoisomerase iv. the antibacterial properties of selected gyrb inhibitors were demonstrated by their activity against staphylococcus aureus and enterococcus faecalis in the low micromolar range. the most promising compounds, 8a and 13e, inhibited escherichia coli and s. aure ... | 2017 | 28621824 |
| spectrum and antibiotic resistance of uropathogens between 2004 and 2015 in a tertiary care hospital in hungary. | surveillance of the bacterial spectrum and antibiotic-resistance patterns of locally occurring uropathogens is essential to serve as a basis for empirical treatment of urinary tract infections (utis), as antibiotic-resistance rates may vary geographically with significant differences between countries and regions, and with time. | 2017 | 28621642 |
| evaluation of free or anchored antimicrobial peptides as candidates for the prevention of orthopaedic device-related infections. | the prevention of implant-associated infection, one the most feared complications in orthopaedic surgery, remains a major clinical challenge and urges development of effective methods to prevent bacterial colonization of implanted devices. alpha-helical antimicrobial peptides (amps) may be promising candidates in this respect due to their potent and broad-spectrum antimicrobial activity, their low tendency to elicit resistance and possible retention of efficacy in the immobilized state. the aim ... | 2017 | 28707817 |
| infectious crystalline keratopathy after descemet’s stripping endothelial keratoplasty. | a 68-year-old woman presented with infectious crystalline keratopathy 4 months after she underwent a combined phacoemulsification and descemet's stripping endothelial keratoplasty for fuch's endothelial dystrophy in her left eye. after 5 months of topical moxifloxacin 1%, the infiltrate responded well but had not completely resolved, with the resulting endothelial failure requiring a penetrating keratoplasty 9 months after the initial operation. microbiology identified enterococcus faecalis with ... | 2017 | 28619977 |
| draft genome sequences of six enterococcus faecalis strains isolated from malaysian clinical and environmental origins. | enterococcus faecalis is known to cause a variety of nosocomial infections, including urinary tract infections. antibiotic resistance and virulence properties in this species are of public concern. the draft genome sequences of six e. faecalis strains isolated from clinical and environmental sources in malaysia are presented here. | 2017 | 28619812 |
| evaluation of antimicrobial activity of association of chlorhexidine to photosensitizer used in photodynamic therapy in root canals infected by enterococcus faecalis. | the aim of the present study was to evaluate, in vitro, the influence of the addition of chlorhexidine to photosensitiser in the antimicrobial activity of photodynamic therapy in root canals infected by enterococcus faecalis. | 2017 | 28619615 |
| triterpenoid saponins and other glycosides from the stems and bark of jaffrea xerocarpa and their biological activity. | six previously undescribed triterpenoid saponins and two previously undescribed norlupane triterpenes were isolated with five known saponins, three known lupane derivatives, 17,20-didehydro-20-deoxyjujubogenin, rutin, (±) 3α-o-β-d-glucopyranosyl-lyoniresinol, (±) 4-o-β-d-glucopyranosyl-maesopsin, three phenol glycosides, and uridine from the stems and bark of jaffrea xerocarpa (baill.) h. c. hopkins & pillon (= basionym alphitonia xerocarpus baill.) (rhamnaceae), an endemic tree of new caledonia ... | 2017 | 28614728 |
| signal biosynthesis inhibition with ambuic acid as a strategy to target antibiotic-resistant infections. | there has been major interest by the scientific community in antivirulence approaches against bacterial infections. however, partly due to a lack of viable lead compounds, antivirulence therapeutics have yet to reach the clinic. here we investigate the development of an antivirulence lead targeting quorum sensing signal biosynthesis, a process that is conserved in gram-positive bacterial pathogens. some preliminary studies suggest that the small molecule ambuic acid is a signal biosynthesis inhi ... | 2017 | 28607020 |
| bacterial sepsis in neonates: single centre study in a neonatal intensive care unit in bosnia and herzegovina. | the aim of the study was to evaluate the incidence, mortality, risk factors, aetiology and the susceptibility to antibiotics of the bacteria responsible for sepsis. | 2017 | 28605923 |
| postoperative enterococcus faecalis endophthalmitis: virulence factors leading to poor visual outcome. | purpose postoperative endophthalmitis caused by enterococcus faecalis often results in poor visual outcome due to retinal destruction. we evaluated the virulence factors of enterococcus faecalis clinical isolates and analyzed their relationship with retinal destruction and poor visual outcomes in postoperative bacterial endophthalmitis (pbe) following cataract surgery. | 2017 | 28707018 |
| characterization and application of antilisterial enterocins on model fresh cheese. | enterococcus faecalis strains isolated from an artisanal cheese were selected based on enterocin production against listeria monocytogenes. the strains formed biofilms and presented high hydrophobic character and good autoaggregation and coaggregation capacity with l. monocytogenes. strains l3a21m3 and l3b1k3 presented high survival under gastrointestinal conditions, were able to adhere to human intestinal cells (caco-2 and ht-29), and blocked the adhesion and invasion of l. monocytogenes. the a ... | 2017 | 28703625 |
| restructuring of enterococcus faecalis biofilm architecture in response to antibiotic-induced stress. | bacterial biofilms are intrinsically resistant to antimicrobial treatment, which contributes to microbial persistence in clinical infections. enterococcus faecalis is an opportunistic pathogen that readily forms biofilms and is the most prevalent enterococcal species identified in healthcare-associated infections. since intrinsic resistance to multiple antibiotics is common for enterococci, and antibiotic resistance is elevated in biofilm populations, it is imperative to understand the mechanism ... | 2017 | 28685097 |
| draft genome sequence of enterococcus faecalis atcc baa-2128. | while a part of the native gut microflora, the gram-positive bacterium enterococcus faecalis can lead to serious infections elsewhere in the body. the draft genome of e. faecalis strain atcc baa-2128, isolated from piglet feces, was examined. this draft genome consists of 42 contigs, 12 of which exhibit homology to annotated plasmids. | 2017 | 28684576 |
| a loop-mediated isothermal amplification (lamp) assay for the rapid detection of enterococcus spp. in water. | faecal pollution of water and the resulting potential presence of human enteric pathogens is a predominant threat to public health. microbiological water quality can be assessed by the detection of standard faecal indicator bacteria (sfib) such as e. coli or certain enterococcus species. in recent years, isothermal amplification methods have become a useful alternative to polymerase chain reaction (pcr), allowing molecular diagnostics with simple or no instrumentation. in this study, a novel scr ... | 2017 | 28591662 |
| an entirely solid phase peptide synthesis-based strategy for synthesis of gelatinase biosynthesis-activating pheromone (gbap) analogue libraries: investigating the structure-activity relationships of the enterococcus faecalis quorum sensing signal. | the development of an entirely solid-phase peptide synthesis (spps)-based synthesis of the quorum sensing signal gelatinase biosynthesis-activating pheromone (gbap) from enterococcus faecalis is reported. the method was used to prepare three libraries of analogues to investigate the structure-activity relationships (sars) of the gbap signal. the sar studies revealed new characteristics of the gbap signal and uncovered the most potent quorum sensing activator in e. faecalis known to date. | 2017 | 28590764 |