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telavancin activity in vitro tested against a worldwide collection of gram-positive clinical isolates (2014).telavancin activity was measured against gram-positive pathogens collected worldwide during 2014 using the revised broth microdilution testing method. the results were compared with previous reports.201728735051
in vitro stepwise selection of reduced susceptibility to lipoglycopeptides in enterococci.the propensity of oritavancin to select for stably elevated oritavancin minimum inhibitory concentrations (mics) was studied by serial passaging of strains in broth containing oritavancin for 20days. seven clinical strains of enterococcus faecalis and e. faecium were studied; they included vancomycin-susceptible and both vana and vanb vancomycin-resistant isolates. stepwise oritavancin selection yielded stably elevated oritavancin mics in six of the seven strains, with mic increases ranging from ...201728733126
hypochlorite solution for root canal irrigation that lacks a chlorinated odor.this is an in vitro study to develop a formulation of a hypochlorite solution for root canal irrigation that lacks a chlorinated odor. the antibacterial effect, tissue dissolution efficacy, and the cytotoxicity of the solution were assessed in cell culture and were compared with those of commercial sodium hypochlorite (naocl) solutions.201728729797
in vitro activity of telavancin against clinically important gram-positive pathogens from 69 u.s. medical centers (2015): potency analysis by u.s. census divisions.a total of 8,072 gram-positive isolates collected from 69 medical centers among all 9 u.s. census divisions during the 2015 sentry antimicrobial surveillance program were included. telavancin had minimal inhibitory concentration (mic)50 and mic90 values of 0.03/0.06 μg/ml, respectively, against methicillin-susceptible (mssa) and methicillin-resistant staphylococcus aureus (mrsa). similar activity was also observed among coagulase-negative staphylococci (mic50/90, 0.03/0.06 μg/ml; 100.0% inhibite ...201728727951
soymilk fermentation by enterococcus faecalis vb43 leads to reduction in the immunoreactivity of allergenic proteins β-conglycinin (7s) and glycinin (11s).food allergies represent a serious problem affecting human health and soy proteins rank among the most allergenic proteins from food origin. the proteolytic enzymes produced by lactic acid bacteria (lab) can hydrolyse the major allergens present in soybean, reducing their immunoreactivity. many studies have reported the ability of lab to ferment soy-based products; while the majority of them focus on the improvement of the sensory characteristics and functionality of soy proteins, a lack of info ...201728726509
[distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015: results from a multicenter, retrospective study].objective: to describe the distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015 to provide reference for empirical anti-infection treatment. methods: pathogens were from hematology department of 26 tertiary hospitals in jiangsu province from 2014 to 2015. antimicrobial susceptibility testing was carried out according to a unified protocol using kirby-bauer method or agar dilution method. collection of drug susceptibility results and corresp ...201728810329
efficacy of radiant catalytic ionization to reduce bacterial populations in air and on different surfaces.air contamination by biological agents is often observed in medical or veterinary facilities and industrial plants. bioaerosols may sediment and pose the surface contamination. microorganisms present on them may become a source of infections among humans and food contamination. this study determined the use of oxidative gases, including ozone and peroxide, generated by the radiant catalytic ionization (rci) cell for the inactivation of acinetobacter baumannii, escherichia coli, enterococcus faec ...201728803189
antimicrobial activities of widely consumed herbal teas, alone or in combination with antibiotics: an in vitro study.because of increasing antibiotic resistance, herbal teas are the most popular natural alternatives for the treatment of infectious diseases, and are currently gaining more importance. we examined the antimicrobial activities of 31 herbal teas both alone and in combination with antibiotics or antifungals against some standard and clinical isolates of pseudomonas aeruginosa, acinetobacter baumannii, escherichia coli, klebsiella pneumoniae, enterococcus faecalis, methicillin susceptible/resistant s ...201728761777
antimicrobial susceptibility among gram-positive and gram-negative organisms collected from the latin american region between 2004 and 2015 as part of the tigecycline evaluation and surveillance trial.the in vitro activity of tigecycline and comparator agents was evaluated against gram-positive and gram-negative isolates collected in latin american centers between 2004 and 2015 as part of the tigecycline evaluation and surveillance trial (t.e.s.t.) global surveillance study.201728701170
investigation and analysis of the characteristics and drug sensitivity of bacteria in skin ulcer infections.skin ulcer is a common type of disease affecting patients' health and quality of life, and bacterial infection increases the difficulty of its management.201728689800
patterns of multi-drug resistant bacteria at first culture from patients admitted to a third level university hospital in calabria from 2011 to 2014: implications for empirical therapy and infection control.surveillance of antimicrobial drug resistance is fundamental to guide empirical treatment. however, the european antimicrobial resistance surveillance network provides a general picture, which might not be applicable to clinical settings that are excluded from this survey. we evaluated resistance patterns of eskape isolates over a four-year period in a third level university hospital in the province of catanzaro (southern italy). in this retrospective study, we evaluated the frequency of eskape ...201728603227
evaluation of the in vitro antimicrobial activity of selected saudi scorpion venoms tested against multidrug-resistant micro-organisms.scorpion venoms are a rich source of bioactive peptides with promising clinical value that may lead to the discovery and development of new drugs. the present study was designed to evaluate the in vitro antimicrobial activities of the venoms extracted from three medically important saudi scorpions (androctonus crassicauda, androctonus bicolor and leiurus quinquestriatus).201728587870
antimicrobial efficacy of triple antibiotic-eluting polymer nanofibers against multispecies biofilm.the elimination of microbial flora in cases of immature permanent teeth with necrotic pulp is both key and a challenging goal for the long-term success of regenerative therapy. recent research has focused on the development of cell-friendly intracanal drug delivery systems. this in vitro study aimed to investigate the antimicrobial action of 3-dimensional (3d) tubular-shaped triple antibiotic-eluting nanofibrous constructs against a multispecies biofilm on human dentin. polydioxanone polymer sol ...201728778504
disulfide bonds: a key modification in bacterial extracytoplasmic proteins.disulfide bonds are a common posttranslational modification that contributes to the folding and stability of extracytoplasmic proteins. almost all organisms, from eukaryotes to prokaryotes, have evolved enzymes to make and break these bonds. accurate and efficient disulfide bond formation can be vital for protein function; therefore, the enzymes that catalyze disulfide bond formation are involved in multiple biological processes. recent advances clearly show that oral bacteria also have the abil ...201728797211
physicochemical characterization of water-soluble chitosan derivatives with singlet oxygen quenching and antibacterial capabilities.new water-soluble chitosan derivatives (wsch) were obtained by maillard reaction (mr) between glucosamine (ga) with both low and medium molecular weight chitosans (ch). the wsch showed larger solubility than the respective ch, while their deacetylation degree (dd) decreased by approximately 12%. infrared spectroscopy experiments of wsch confirmed the formation of imine bonds after mr with intensified pyranose structure, and sugar molecules as polymer branches. however, a 6-times reduction of the ...201728396271
ultraviolet-c light as a means of disinfecting anesthesia workstations.anesthesia workstations (aws) are a reservoir for pathogenic organisms potentially associated with surgical site infections. this study examined the effectiveness of the tru-d smartuvc device (tru-d llc, nashville, tn) on bioburden reduction (br) on aws.201728431851
do quaternary ammonium monomers induce drug resistance in cariogenic, endodontic and periodontal bacterial species?antibacterial monomers were developed to combat oral biofilm acids and caries; however, little is known on whether quaternary ammonium monomers (qams) would induce drug resistance in oral bacteria. the objective of this study was to investigate the effects of new antimicrobial monomers dimethylaminohexadecyl methacrylate (dmahdm) and dimethylaminododecyl methacrylate (dmaddm) on the induction of drug resistance in eight species of cariogenic, endodontic and periodontal bacteria for the first tim ...201728755761
validity of somatic cell count as indicator of pathogen-specific intramammary infections.the objective of this study was to determine whether somatic cell count (scc) was an effective test, with a sensitivity exceeding 85%, to determine species-specific bacterial infections. in addition, the relation between the scc and various udder pathogen groups was investigated. scc thresholds of greater than 200 000 cells/ml were used in quarter and greater than 150 000 cells/ml in composite milk samples. a retrospective study was conducted on a data set for 89 635 quarter and 345 467 composit ...201728470079
the curious case of achromobacter eurydice, a gram-variable pleomorphic bacterium associated with european foulbrood disease in honeybees.honeybees are prone to parasite and pathogen infestations/infections due to their social colony life. bacterial pathogens in particular lead to destructive infections of the brood. european foulbrood is caused by the bacterium melissococcus plutonius in combination with several other gram-positive bacteria (achromobacter eurydice, bacillus pumilus, brevibacillus laterosporus, enterococcus faecalis, paenibacillus alvei, paenibacillus dendritiformis) involved as secondary invaders following the in ...201728634639
chemical constituents of helichrysum italicum (roth) g. don essential oil and their antimicrobial activity against gram-positive and gram-negative bacteria, filamentous fungi and candida albicans.the aerial parts of helichrysum italicum (roth) g. don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. the essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). the most o ...201728725151
1,1ꞌ-carbonyldiimidazole (cdi) mediated facile synthesis, structural characterization, antimicrobial activity and in-silico studies of coumarin-3-carboxamide derivatives.despite of the availability of variety of antibacterial agents, re-emergance of pathogenic bacteria is still a serious medical concern. so, identification of new, safer, and selective antibacterial agents is the key interest in the medicinal chemistry research.201728641525
cytotoxic and antimicrobial activities of cantharellus cibarius fr. (cantarellaceae).antibacterial and cytotoxic activities of cyclohexane, dichloromethane, methanol, and aqueous extracts of cantharellus cibarius were tested. broth microdilution assay was performed against 10 bacterial strains (staphylococcus aureus, s. epidermidis, micrococcus luteus, bacillus subtilis, enterococcus feacalis, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella abony), with emphasis on helicobacter pylori. methanol extract was the most active against h. pylori strains wit ...201728613963
antibacterial and anticancer activities of acetone extracts from in vitro cultured lichen-forming fungi.lichens that were used in traditional medicine for ages produce numerous secondary metabolites, however our knowledge about biological activities of substances secreted by separated bionts is scarce. the main objectives of this study were to isolate and find optimal conditions for the growth of mycelia from three common lichen-forming fungi, i.e. caloplaca pusilla, protoparmeliopsis muralis and xanthoria parietina and to evaluate antibacterial and antiproliferative activities of their acetone ex ...201728592323
comparative evaluation of antimicrobial efficacy of mtad, 3% naoci and propolis against e faecalis.the present study sought to compare the antimicrobial efficacy of 3% naocl, biopure mtad (tulsa dentsply, tulsa, ok) and brazilian ethanolic extract of propolis (eep) against enterococcus faecalis (e. faecalis).201727625552
antimicrobial activity of aspilia latissima (asteraceae).we evaluated the antimicrobial activity of aspilia latissima - an abundant plant from the brazilian pantanal region - against candida albicans, candida parapsilosis, candida krusei, candida tropicalis, pseudomonas aeruginosa, enterococcus faecalis, escherichia coli and staphylococcus aureus. the crude extracts and fractions showed activity in all tested microorganisms. the chloroform fraction of the leaves and roots showed the most antimicrobial activity against s. aureus, with an mic of 500 μg/ ...201726691468
antimicrobial activity of aqueous and organic extracts of a saudi medicinal plant: rumex nervosus.the antimicrobial effect of aerial part of rumex nervosus obtained from the southern region of saudi arabia was evaluated on bacterial strains staphylococcus aureus, methicillin resistant s. aureus, enterococcus faecalis, escherichia coli, pseudomonas aeruginosa, and fungal strain candida albicans.201726681888
in vitro antimicrobial and cytotoxic effects of anacardium occidentale and mangifera indica in oral care.oral health is an integral and important component of general health. infectious diseases such as caries, periodontal, and gingivitis indicate the onset of imbalance in homeostasis between oral micro biota and host. the present day medicaments used in oral health care have numerous side effects. the uses of herbal plants as an alternative have gained popularity due to side effects of antibiotics and emergence of multidrug resistant strains. anacardium occidentale (cashew) and mangifera indica (m ...201725709341
evaluation of minimal inhibitory concentration of two new materials using tube dilution method: an in vitro study.the aim of the study is to evaluate and compare the antimicrobial efficacy of two new materials mta plus and biodentine with proroot mta using tube dilution method.201725829698
comparative evaluation of antimicrobial efficacy of qmix™ 2 in 1, sodium hypochlorite, and chlorhexidine against enterococcus faecalis and candida albicans.the aim of this study is to compare the antimicrobial efficacy of qmix™ 2 in 1, sodium hypochlorite (naocl), and chlorhexidine (chx) against enterococcus faecalis and candida albicans.201725829691
evaluation of antibacterial and antifungal activity of new calcium-based cement (biodentine) compared to mta and glass ionomer cement.to evaluate the antibacterial and antifungal properties of calcium-based cement, biodentine (ca3sio2), compared to commercial glass ionomer cements (gics) and mineral trioxide aggregate (mta).201725657526
effect of zingiber officinale and propolis on microorganisms and endotoxins in root canals.the purpose of this study was to evaluate the effectiveness of glycolic propolis (pro) and ginger (gin) extracts, calcium hydroxide (ch), chlorhexidine (clx) gel and their combinations as icms (icms) against candida albicans, enterococcus faecalis, escherichia coli and endotoxins in root canals.201723559108
in vitro antimicrobial activity of auxiliary chemical substances and natural extracts on candida albicans and enterococcus faecalis in root canals.the aim of this study was to evaluate the antimicrobial activity of auxiliary chemical substances and natural extracts on candida albicans and enterococcus faecalis inoculated in root canals.201723739849
influence of sucrose on growth and sensitivity of candida albicans alone and in combination with enterococcus faecalis and streptococcus mutans to photodynamic therapy.this study has evaluated the effects of photodynamic inactivation (pdi) using erythrosine as photosensitizer and green light-emitting diode (led) on biofilms of candida albicans alone and in combination with enterococcus faecalis and streptococcus mutans. we have also evaluated the effect of sucrose on biofilm formation and bacterial growth and sensitivity to pdi. biofilms were formed in suspension of 10(6) cells/ml on plates before being grown in broth culture with and without sucrose and incub ...201728389898
evaluation of the role of probiotics in endodontic treatment: a preliminary study.the principal goal of endodontics is the prevention of periapical infection. acute and chronic apical periodontitis occur due to the persistence of pathogenic microorganisms such as enterococcus faecalis and candida albicans harboring the root canal systems of the teeth. the concept of the use of probiotics in addressing endodontic disease is new and has not been studied adequately. on the basis of the success of probiotics in periodontal treatment, this preliminary work was performed (a) to eva ...201728316949
evaluation of antimicrobial and antifungal efficacy of chitosan as endodontic irrigant against enterococcus faecalis and candida albicans biofilm formed on tooth substrate.bacterial biofilms formed on the root canal wall are often difficult to remove. this study aimed to evaluate the cytotoxic effect and antibacterial efficacy of chitosan when used as root canal irrigant against e. faecalis and candida albicans biofilm formed on tooth substrate.201728298975
additive and reducing effects between calcium hydroxide and current irrigation solutions.microorganisms should be considered to have the major role in starting and perpetuation of pulpo-periapical diseases. using intracanal medicaments is necessary to gain a bacteria-free environment in the canal system. calcium hydroxide (abbreviated as ca(oh)2), which is the most commonly used medicament in endodontic therapy, has been shown to be effective against primary sources of infection; however, its effectiveness against some microorganisms, such as candida albicans and enterococcus faecal ...201728258273
antimicrobial efficacy of probiotic-containing toothpastes: an in vitro evaluation.aim to evaluate, in vitro antimicrobial ability of two probiotic toothpastes (one containing lactobacillus paracasei, other containing lactobacillus acidophilus) and one toothpaste without probiotic separately, and in a combination with two different mouthrinses (one containing essential oils and the other containing hexitidine). methods antimicrobial susceptibility was checked by using the ditch method and clinical laboratory standard institute (clsi) guidelines. two different toothpastes with ...201728165440
biological activities of rosmarinus officinalis l. (rosemary) extract as analyzed in microorganisms and cells.r. officinalis l. is an aromatic plant commonly used as condiment and for medicinal purposes. biological activities of its extract were evaluated in this study, as antimicrobial effect on mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory capacity, and genotoxicity. monomicrobial biofilms of candida albicans, staphylococcus aureus, enterococcus faecalis, streptococcus mutans and pseudomonas aeruginosa and polymicrobial biofilms composed of c. albicans with each bacterium were form ...201728093936
procalcitonin levels in bloodstream infections caused by different sources and species of bacteria.the aim of this study was to evaluate procalcitonin (pct) diagnostic accuracy in discriminating gram-negative (gn) from gram-positive (gp) bloodstream infections and determining the relationship between pct levels, infection sites, and pathogen types.201727979420
antibacterial efficacy of azadirachta indica, mimusops elengi and 2% chx on multispecies dentinal biofilm.to check the antimicrobial activity of azadirachta indica (neem), mimusops elengi (bakul), and chlorhexidine gluconate (chx) on multispecies biofilm of common endodontic pathogens such as streptococcus mutans, enterococcus faecalis, staphylococcus aureus and candida albicans.201726752840
[antibacterial and antifungal activity of salvia apiana against clinically important microorganisms].due to the great global concern regarding bacterial resistance to antibiotics, an ongoing search for new molecules having antibacterial activity is necessary. this study evaluated the antibacterial and anticandidal effects of a hexane extract from the root of salvia apiana. salvia extracts at concentrations of 27, 13.5, 6.8 and 3.4mg/ml caused growth inhibition of staphylococcus aureus, streptococcus pyogenes, enterococcus faecalis and candida albicans. however, no significant effect was observe ...201727595508
microbiologic evaluation of matricaria and chlorhexidine against e. faecalis and c. albicans.to evaluate the antimicrobial activity of different concentrations of matricaria chamomilla and chlorhexidine gel against candida albicans and enterococcus faecalis.201726097333
development of a rapid identification method for the differentiation of enterococcus species using a species-specific multiplex pcr based on comparative genomics.enterococci are lactic acid bacteria that are commonly found in food and in animal gut. since 16 s ribosomal rna (rrna) sequences, genetic markers for bacterial identification, are similar among several enterococcus species, it is very difficult to determine the correct species based on only 16 s rrna sequences. therefore, we developed a rapid method for the identification of different enterococcus species using comparative genomics. we compared 38 genomes of 13 enterococcus species retrieved fr ...201728229213
study on the excision and integration mediated by class 1 integron in enterococcus faecalis.recognized as a mobile genetic element, integron is correlated to the excision and integration of exogenous genes, especially bacterial resistance genes. however, most of the investigations focused on gram-positive bacteria with few exceptions. in this study, enterococcus faecalis was selected to investigate the excision and integration of class 1 integron. a total of eight plasmids were subjected to establish the transformants for excision and integration test. as results showed, positive excis ...201728390978
stimulatory effects of heat-killed enterococcus faecalis on cell-mediated immunity in fish.intracellular bacterial and viral diseases are widespread in the aquaculture industry and cause serious economic losses. development of effective vaccines and adjuvants that can induce cell-mediated immunity is urgently needed for prevention of these diseases. here we report the immunostimulatory effects of probiotic bacteria ''e. faecalis'' in ginbuna crucian carp carassius auratus langsdorfii. intraperitoneal injection of heat-killed e. faecalis induced an increase in cd4-1(+) lymphocytes, cd8 ...201728389237
antagonistic activity of lactobacillus reuteri strains on the adhesion characteristics of selected pathogens.adhesion ability of probiotics is the key factor that decides their colonization in the gastrointestinal tract and potential to inhibit pathogens. therefore, adhesion ability can be considered as a key determinant for probiotic efficacy. presents study documents the antagonistic activity of viable/untreated, lithium chloride (licl) treated or heat-killed forms of eight probiotic lactobacillus reuteri strains on the adhesion characteristics of selected pathogens. all strains investigated were abl ...201728377765
poly(methyl methacrylate) surface modification for surfactant-free real-time toxicity assay on droplet microfluidic platform.microfluidic droplet reactors have many potential uses, from analytical to synthesis. stable operation requires preferential wetting of the channel surface by the continuous phase which is often not fulfilled by materials commonly used for lab-on-chip devices. here we show that a silica nanoparticle (sinp) layer coated onto a poly(methyl methacrylate) (pmma) and other thermoplastics surface enhances its wetting properties by creating nanoroughness, and allows simple grafting of hydrocarbon chain ...201728375597
identification and prevalence of tetracycline resistance in enterococci isolated from poultry in ilishan, ogun state, nigeria.tetracycline is one of the most frequently used antibiotics in nigeria both for human and animal infections because of its cheapness and ready availability. the use of tetracycline in animal husbandry could lead to horizontal transfer of tet genes from poultry to human through the gut microbiota, especially enterococci. therefore, this study is designed to identify different enterococcal species from poultry feces in selected farms in ilishan, ogun state, nigeria, determine the prevalence of tet ...201726957873
exogenous endophthalmitis caused by enterococcus casseliflavus: a case report and discussion regarding treatment of intraocular infection with vancomycin-resistant enterococci.endophthalmitis caused by enterococci is rare, and cases involving vancomycin-resistant enterococci are even more so. due to the poor bioavailability of many antibiotics in the vitreous chamber, special considerations are required when choosing antibiotics to treat these infections. the authors report the first case of exogenous endophthalmitis caused by enterococcus casseliflavus via the unique mechanism of high-velocity water stream trauma from a toy water gun. a previously healthy four-year o ...201726744592
a 10-year profile of enterococcal bloodstream infections at a tertiary-care hospital in japan.the first aim of this study is to characterize the epidemiology of enterococcal bloodstream infections (bsis) at a japanese tertiary-care hospital. the second aim is to identify predictive factors for 30-day mortality.201728385565
safety assessment of commensal enterococci from dogs.enterococci form a complex, diverse, and very important group of bacteria from the technological and food safety aspect, or from the health-improving aspect as probiotics. generally, enterococci are considered to be of low pathogenic potential, which is associated mostly with clinical strains. in these strains, production of virulence factors as well as resistance to many antimicrobial drugs could complicate treatment of nosocomial infections. because there is a lack of information on incidence ...201728316009
maldi-tof mass spectrometry as a useful tool for identification of enterococcus spp. from wild birds and differentiation of closely related species.the aim of this study was to explore the accuracy and feasibility of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) in identifying bacteria from environmental sources, as compared to rpoa gene sequencing, and to evaluate the occurrence of bacteria of the genus enterococcus in wild birds. in addition, a phyloproteomic analysis of certain enterococcus species with spectral relationships was performed. the enterococci were isolated from 25 species of wil ...201728285496
comparative genomics of enterococcus spp. isolated from bovine feces.enterococcus is ubiquitous in nature and is a commensal of both the bovine and human gastrointestinal (gi) tract. it is also associated with clinical infections in humans. subtherapeutic administration of antibiotics to cattle selects for antibiotic resistant enterococci in the bovine gi tract. antibiotic resistance genes (args) may be present in enterococci following antibiotic use in cattle. if located on mobile genetic elements (mges) their dissemination between enterococcus species and to pa ...201728270110
antimicrobial resistance and virulence profile of enterococci isolated from poultry and cattle sources in nigeria.this study investigated the occurrence, antimicrobial resistance and virulence of enterococcus from poultry and cattle farms. three hundred and ninety samples: cloacal/rectal swabs (n = 260) and manure (n = 130] were processed for recovery of enterococcus species. standard bacteriological methods were used to isolate, identify and characterize enterococcus species for antimicrobial susceptibility and expression of virulence traits. detection of antibiotic resistance and virulence genes was carri ...201727987112
[profile of bacterial resistance in pediatric urinary tract infections in 2014].in pediatric units, bacteria-producing extended-spectrum-betalactamase (esbl) have an increasing prevalence among bacteria causing febrile urinary tract infections (utis). the purpose of this study was to evaluate the epidemiology of bacteria resistance patterns observed in utis, in order to assess the current antibiotic treatment protocols. this study is based upon a single-center retrospective chart review of the cytobacteriological urine cultures performed in utis between 1 january and 31 dec ...201728131557
effect of preoperative biliary drainage on bacterial flora in bile of patients with periampullary cancer.patients with obstructive jaundice due to periampullary tumours may undergo preoperative biliary drainage (pbd). the effect of pbd on the microbiome of the biliary system and on postoperative outcome remains unclear.201728121036
searching for a potential antibacterial lead structure against bacterial biofilms among new naphthoquinone compounds.the aims of this study were to design, synthesize and to evaluate 2-hydroxy-3-phenylsulfanylmethyl-[1,4]-naphthoquinones against gram-negative and gram-positive bacterial strains, including methicillin-resistant staphylococcus aureus (mrsa) and its biofilm, to probe for potential lead structures.201727930849
evaluation of dental adhesive systems incorporating an antibacterial monomer eugenyl methacrylate (egma) for endodontic restorations.the purpose of this study was to incorporate egma, an antibacterial monomer into two commercial dental adhesive systems for their application in endodontic restoration with the aim to disinfect the root canal space before curing and to inhibit bacterial growth on their surfaces after being cured.201728245928
n-acetylcysteine inhibits growth, adhesion and biofilm formation of gram-positive skin pathogens.n-acetylcysteine (nac) is a non-antibiotic drug with antimicrobial properties against biofilm phenotypes of gram-positive and gram-negative bacteria. our aim was to assess the effects of nac on the growth of gram-positive human skin and mucous membrane pathogens in the planktonic and biofilm phases. the minimum inhibitory concentrations (mics) of nac against enterococcus faecalis, corynebacterium ammoniagenes, mycobacterium smegmatis, propionibacterium acnes, staphylococcus aureus, s. epidermidi ...201728237766
prenylated flavonoids from the stems and roots of tripterygium wilfordii.phytochemical investigation on the stems and roots of tripterygium wilfordii led to the isolation and characterization of three new prenylated flavanones, tripteryols a-c (1-3), along with (±)-5,4'-dihydroxy-2'-methoxy-6',6″-dimethypyraro-(2″,3″:7,8)-6-methyflavanone (4), and ((2s)-5,7,4'-trihydroxy-2'-methoxy-8,5'-di(3-methyl-2-butenyl)-6-methylflavanone (5). structures of the compounds 1-5 were elucidated using spectroscopic techniques, such as uv, ir, nmr (1d and 2d), and hresi-ms. tripteryol ...201728389278
the safe enterocin dd14 is a leaderless two-peptide bacteriocin with anti-clostridium perfringens activity.enterococcus faecalis 14, a strain previously isolated from meconium, displayed activity against four clostridium perfringens isolates when co-cultured on agar plates. the anti-clostridium activity was ascribed to the production of enterocin dd14, which was subsequently purified. the minimum inhibitory concentration (mic) of enterocin dd14 against one collection strain and one clinical c. perfringens strain was determined at 50 µg/ml. furthermore, using the intestinal epithelial cell line ipec-1 ...201728104423
enterocin b3a-b3b produced by lab collected from infant faeces: potential utilization in the food industry for listeria monocytogenes biofilm management.enterococcus faecalis b3a-b3b produces the bacteriocin b3a-b3b with activity against listeria monocytogenes, staphylococcus aureus, methicillin-resistant staphylococcus aureus (mrsa) and clostridium perfringens, but apparently not against fungi or gram-negative bacteria, except for salmonella newport. b3a-b3b enterocin has two different nucleotides but similar amino acid composition to the class iib mr10a-mr10b enterocin. b3a-b3b consists of two peptides of predicted molecular mass of 5176.31 da ...201727878401
postmortem in vitro ethanol production-it could be more common than we think!the blood alcohol concentration (bac) is the most frequent determination in a forensic toxicology laboratory. despite its apparent simplicity, the results interpretation can be complex and always have relevant social and legal implications, particularly in postmortem analysis. in the present report we describe the case of a 55-year-old male with an apparent natural death by myocardial infarction, whose initial bac was 0.18g/l but, in repeated determinations prompted by discrepancies observed in ...201728108144
prevalence of multidrug resistant uropathogenic bacteria in pediatric patients of a tertiary care hospital in eastern india.today, because systemic infections such as urinary tract infection (uti) affect even pediatric patients, antibiotic resistant bacteria have become a constant clinical challenge. in the present study, a total of 1054 urine samples were collected from pediatric patients over 18 months. from these samples, 510 isolates of pathogenic bacteria were collected using hicrome uti agar. antibiotic sensitivity tests of isolates were performed using the kirby-bauer method. two gram-positive bacteria (entero ...201726617250
branimycins b and c, antibiotics produced by the abyssal actinobacterium pseudonocardia carboxydivorans m-227.two new antibiotics, branimycins b (2) and c (3), were produced by fermentation of the abyssal actinobacterium pseudonocardia carboxydivorans m-227, isolated from deep seawater of the avilés submarine canyon. their structures were elucidated by hrms and nmr analyses. these compounds exhibit antibacterial activities against a panel of gram-positive bacteria, including corynebacterium urealyticum, clostridium perfringens, and micrococcus luteus, and against the gram-negative bacterium neisseria me ...201728169531
purification, characterization and functional analysis of the immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus and their antibiofilm properties.the present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus (pp-lec). the pp-lec was purified by affinity chromatography with mannose coupled sepharose cl-4b column and it exhibits single band with a molecular weight of 155 kda in sds-page. the surface morphology of purified pp-lec displays the homogeneous nature of protein. a distinct peak with a retention time of 3.3 min was appeared in high performance liqui ...201728110033
abietane diterpenes from the cones of abies numidica de lannoy ex carrière (pinaceae) and in vitro evaluation of their antimicrobial properties.eight known abietane diterpenes (1-8) were isolated for the first time from abies numidica cones (pinaceae). the structures of all compounds were established by means of 1d and 2d nmr, and uv spectral analyses. the hydromethanolic extract of a. numidica cones was tested for its antimicrobial activity against 17 gram-positive and gram-negative bacteria and against five yeasts by the use of liquid and solid medium and bioautography methods. the best antimicrobial activity was found against gram-po ...201727237830
a critical role for extracellular dna in dental plaque formation.extracellular dna (edna) has been identified in the matrix of many different monospecies biofilms in vitro, including some of those produced by oral bacteria. in many cases, edna stabilizes the structure of monospecies biofilms. here, the authors aimed to determine whether edna is an important component of natural, mixed-species oral biofilms, such as plaque on natural teeth or dental implants. to visualize edna in oral biofilms, approaches for fluorescently stained edna with either anti-dna ant ...201727770039
functional studies of e. faecalis rnase j2 and its role in virulence and fitness.post-transcriptional control provides bacterial pathogens a method by which they can rapidly adapt to environmental change. dual exo- and endonucleolytic activities of rnase j enzymes contribute to gram-positive rna processing and decay. first discovered in bacillus subtilis, rnase j1 plays a key role in mrna maturation and degradation, while the function of the paralogue rnase j2 is largely unknown. previously, we discovered that deletion of the enterococcus faecalis rnjb gene significantly att ...201728384222
cationic net charge and counter ion type as antimicrobial activity determinant factors of short lipopeptides.to get a better insight into the antimicrobial potency of short cationic lipopeptides, 35 new entities were synthesized using solid phase peptide strategy. all newly obtained lipopeptides were designed to be positively charged from +1 to +4. this was achieved by introducing basic amino acid - lysine - into the lipopeptide structure and had a hydrophobic fatty acid chain attached. lipopeptides were subjected to microbiological tests using reference strains of gram-negative bacteria: escherichia c ...201728203232
d-amino acids reduce enterococcus faecalis biofilms in vitro and in the presence of antimicrobials used for root canal treatment.enterococcus faecalis is the most frequent species present in post-treatment disease and plays a significant role in persistent periapical infections following root canal treatment. its ability to persist in stressful environments is inter alia, due to its ability to form biofilms. the presence of certain d-amino acids (daas) has previously been shown to reduce formation of bacillus subtilis biofilms. the aims of this investigation were to determine if daas disrupt biofilms in early and late gro ...201728151960
combined chemical and physical transformation method with rbcl and sepiolite for the transformation of various bacterial species.dna transformation that delivers plasmid dnas into bacterial cells is fundamental in genetic manipulation to engineer and study bacteria. developed transformation methods to date are optimized to specific bacterial species for high efficiency. thus, there is always a demand for simple and species-independent transformation methods. we herein describe the development of a chemico-physical transformation method that combines a rubidium chloride (rbcl)-based chemical method and sepiolite-based phys ...201728185866
hydrocarbon-stapled lipopeptides exhibit selective antimicrobial activity.antimicrobial peptides (amps) occur widely in nature and have been studied for their therapeutic potential. amps are of interest due to the large number of possible chemical structural combinations using natural and unnatural amino acids, with varying effects on their biological activities. using physicochemical properties from known naturally occurring amphipathic cationic amps, several hydrocarbon-stapled lipopeptides (hslps) were designed, synthesized, and tested for antimicrobial properties. ...201728073163
evaluation of health potential of nutritionally enriched kodo millet (eleusine coracana) grown in himachal pradesh, india.in this study, kodo millet grains were phytochemically investigated for their nutritional and antioxidant potential for their use as functional foods. methanolic extracts of grains showed higher phenolic content and antioxidant activity. tlc studies of the extracted polyphenols from kodo millet showed the predominant presence of ferulic acid and cinnamic acid in the millet. further quantification of these polyphenols was done by using hplc, analysing ferulic acid and cinnamic acid. antagonistic ...201727507461
antibacterial, antioxidant and anti-proliferative properties and zinc content of five south portugal herbs.crataegus monogyna l. (rosaceae) (cm), equisetum telmateia l. (equisataceae) (et), geranium purpureum vil. (geraniaceae) (gp), mentha suaveolens ehrh. (lamiaceae) (ms), and lavandula stoechas l. spp. luisieri (lamiaceae) (ls) are all medicinal.201727925492
identification of the lyso-form n-acyl intramolecular transferase in low-gc firmicutes.bacterial lipoproteins are embedded in the cell membrane of both gram-positive and gram-negative bacteria where they serve numerous functions central to cell envelope physiology. lipoproteins are tethered to the membrane by an n-acyl-s-(mono/di)-acyl-glyceryl-cysteine anchor that is variably acylated depending on genera. in several low-gc gram-positive firmicutes, a monoacyl-glyceryl-cysteine with an n-terminal fatty acid (known as the lyso form) has been reported, though how it is formed is unk ...201728320885
the antibacterial activity of extracts of nine plant species with good activity against escherichia coli against five other bacteria and cytotoxicity of extracts.the development of antibiotic resistant bacteria stems from a number of factors, including inappropriate use of antibiotics in human and animal health and their prolonged use as growth promoters at sub-clinical doses in poultry and livestock production. we were interested in investigating plants that could be useful in protecting humans or animals against diarrhoea. we decided to work on extracts of nine plant species with good activity against escherichia coli based on earlier work in the phyto ...201728241818
epidihydropinidine, the main piperidine alkaloid compound of norway spruce (picea abies) shows promising antibacterial and anti-candida activity.this study reports for the first time promising antibacterial and antifungal effects of epidihydropinidine, the major piperidine alkaloid in the needles and bark of norway spruce, picea abies (l.) karsten. epidihydropinidine was growth inhibitory against all bacterial and fungal strains used in our investigation, showing the lowest mic value of 5.37μg/ml against pseudomonas aeruginosa, enterococcus faecalis, candida glabrata and c. albicans. epidihydropinidine was nearly three times more active ...201728163074
survival of vaginal microorganisms in three commercially available transport systems.transport systems are used to collect and maintain the viability of microorganisms. two amies media based transport systems, bd cultureswab™ maxv(+) amies medium without charcoal (maxv(+)) and fisherfinest(®) with amies gel transport medium without charcoal (fisherfinest(®)) were compared to a cary-blair media based transport system, starswab(®) anaerobic transport system (starswab(®)), for their capacity to maintain the viability of 17 clinical microorganisms commonly isolated from the vagina ( ...201728242337
the evaluation of cultivable microbiota profile in patients with secondary endodontic infection before and after photo-activated disinfection.secondary/persistent endodontic infection can be the outcome of failure of endodontic treatment. photo-activated disinfection (pad) can be a useful adjunct to mechanical and antimicrobial agents in eliminating endopathogenic microorganisms. in this study, we evaluated the effect of pad on diversity and count of microbiota related to secondary/persistent endodontic infections.201728249746
novel bioactive root canal sealer to inhibit endodontic multispecies biofilms with remineralizing calcium phosphate ions.the objectives of this study were to: (1) develop a bioactive endodontic sealer via dimethylaminohexadecyl methacrylate (dmahdm), 2-methacryloyloxyethyl phosphorylcholine (mpc) and nanoparticles of amorphous calcium phosphate (nacp) for the first time; and (2) evaluate inhibition of early-stage and mature multispecies endodontic biofilm, bond strength to root canal dentine, and calcium (ca) and phosphate (p) ion release.201728223198
the use of bacteriophages to biocontrol oral biofilms.infections induced by oral biofilms include caries, as well as periodontal, and peri-implant disease, and may influence quality of life, systemic health, and expenditure. as bacterial biofilms are highly resistant and resilient to conventional antibacterial therapy, it has been difficult to combat these infections. an innovative alternative to the biocontrol of oral biofilms could be to use bacteriophages or phages, the viruses of bacteria, which are specific, non-toxic, self-proliferating, and ...201728108235
analysis of the expression of nlrp3 and aim2 in periapical lesions with apical periodontitis and microbial analysis outside the apical segment of teeth.to detect the distribution and expression levels of the nucleotide-binding oligomerization domain-like receptor protein 3 (nlrp3) and the absent in melanoma 2 (aim2) inflammasomes in periapical lesions and to analyse the possible microbial stimuli outside of teeth.201728193569
the influence of the autochthonous wastewater microbiota and gene host on the fate of invasive antibiotic resistance genes.the aim of this study was to assess the fate of invasive antibiotic resistance genes (arg) discharged in wastewater. with this objective, antibiotic resistant bacteria (arb) known to harbor specific arg were inoculated in wastewater (hospital effluent, or municipal raw and treated wastewater) and in ultra-pure sterile water microcosms. two sets of wastewater arb isolates were used - set 1, enterococcus faecalis, acinetobacter johnsonii, klebsiella pneumoniae and set 2, enterococcus faecium, acin ...201727697350
evaluating the effect of peptoid lipophilicity on antimicrobial potency, cytotoxicity, and combinatorial library design.growing prevalence of antibiotic resistant bacterial infections necessitates novel antimicrobials, which could be rapidly identified from combinatorial libraries. we report the use of the peptoid library agar diffusion (plad) assay to screen peptoid libraries against the eskape pathogens, including the optimization of assay conditions for each pathogen. work presented here focuses on the tailoring of combinatorial peptoid library design through a detailed study of how peptoid lipophilicity relat ...201728291947
fever and prodromal infections in anti-glomerular basement membrane disease.anti-glomerular basement membrane (gbm) disease is an autoimmune disorder with rapidly progressive glomerulonephritis and alveolar hemorrhage. fever symptom and prodromal infections have been reported in many cases, but still not been elucidated.201728261931
a study of pre-operative presence of micro-organisms in affected knee joints of rheumatoid arthritis patients who need total knee arthroplasty.to evaluate, by way of intraoperative tissue culture and pathological study, the pre-operative presence of micro-organisms in knee joints of patients with rheumatoid arthritis (ra) who need total knee arthroplasty (tka).201727919672
in vitro activity of a polyhexanide-betaine solution against high-risk clones of multidrug-resistant nosocomial pathogens.to determine the in vitro activity of a polyhexanide-betaine solution against collection strains and multidrug-resistant (mdr) nosocomial isolates, including high-risk clones.201727004429
antimicrobial assay and genetic screening of selected freshwater cyanobacteria and identification of a biomolecule dihydro-2h-pyran-2-one derivative.explorations of freshwater cyanobacteria as antimicrobial (bacteria, fungi and methicillin-resistant staphylococcus aureus (mrsa) strains) drug resource using bioassay, nrps (non-ribosomal polypeptide synthetase) and pks (polyketide synthase) genes, as well as in silico approach.201728004519
antimicrobial properties of black grape (vitis vinifera l.) peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds.black grape peel possesses a substantial amount of polyphenolic antimicrobial compounds that can be used for controlling the growth of pathogenic microorganisms. the purpose of this study was to assess antibacterial and antifungal activity of black grape peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds, respectively.201726729960
antibacterial activity of tribulus terrestris and its synergistic effect with capsella bursa-pastoris and glycyrrhiza glabra against oral pathogens: an in-vitro study.in this study, antimicrobial activities of an ethanol extract of tribulus terrestris aloneand in combination with capsella bursa-pastoris and glycyrrhiza glabra were examined in vitro against six pathogens namely streptococcus mutans, streptococcus sanguis, actinomyces viscosus, enterococcus faecalis staphylococcus aureus, and escherichia coli.201726101754
effects of synbiotics on immunity and disease resistance of narrow-clawed crayfish, astacus leptodactylus leptodactylus (eschscholtz, 1823).the aim of this study was to evaluate the effects of prebiotics (mannanoligosaccharide and xylooligosaccharide), probiotics (enterococcus faecalis and pediococcus acidilactici) and synbiotics for 126 days on the immune responses, hemolymph indices, antioxidant enzymes, and biological responses after a 48-hour aeromonas hydrophila exposure of sub-adult crayfish (11.45 ± 1.87 g). most antibacterial activities were observed in the shell mucus of crayfish fed a diet containing xylooligosaccharide +  ...201728363588
molecular viability testing of uv-inactivated bacteria.the polymerase chain reaction (pcr) is effective at detecting bacterial dna in samples, but it is unable to differentiate viable bacteria from inactivated cells or free dna fragments. new pcr-based analytical strategies have been developed to address this limitation. molecular viability testing (mvt) correlates bacterial viability with the ability to rapidly synthesize species-specific ribosomal rna precursor (pre-rrna) in response to brief nutritional stimulation. previous studies demonstrated ...201728283525
drinking water quality and public health in southwestern saudi arabia: the need for a national monitoring program.the aim was to investigate the bacteriological quality of drinking water, and explore the factors involved in the knowledge of the public about the quality of drinking water in najran region, saudi arabia.201725657607
sealing ability of a novel hydrophilic vs. conventional hydrophobic obturation systems: a bacterial leakage study.comparative assessment of apical sealing ability of a novel smart-seal system, resilon, and conventional gutta-percha system using a bacterial leakage model.201725657530
antibacterial and smear layer removal capability of oregano extract solution.the aim of this in vitro study was to evaluate the antimicrobial effect of oregano extract solution (oes) against enterococcus faecalis within root canals and dentin tubules, and its effect on smear layer.201725713479
bactericidal activity of propylene glycol, glycerine, polyethylene glycol 400, and polyethylene glycol 1000 against selected microorganisms.the aim of the present study was to evaluate the bactericidal activity of propylene glycol, glycerine, polyethylene glycol 400 (peg 400), and polyethylene glycol 1000 (peg 1000) against selected microorganisms in vitro.201725992336
apical extrusion of enterococcus faecalis using three different rotary instrumentation techniques: an in vitro study.to compare the apical extrusion of enterococcus faecalis after instrumentation with three different ni-ti rotary instruments- an in vitro study.201725961619
comparison of fluid filtration and bacterial leakage techniques for evaluation of microleakage in endodontics.apical leakage assessment is a way to compare the efficiency of a filling material to seal the apical region of the tooth. many microleakage testing techniques have been introduced through the years, but there has been no agreement as to which technique gives the most accurate results. the aim of this study was to compare the accuracy of fluid filtration and bacterial leakage techniques in the assessment of the apical sealing ability of mineral trioxide aggregate (mta) and calcium enriched mixtu ...201725878674
biofilm forming capacity of enterococcus faecalis on gutta-percha points treated with four disinfectants using confocal scanning laser microscope: an in vitro study.the aim of this study was to evaluate and compare the in vitro biofilm forming capacity of enterococcus faecalis on gutta-percha points disinfected with four disinfectants.201726288622
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