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anti-inflammatory activities of aedes aegypti cecropins and their protection against murine endotoxin shock.mosquitoes are armed with physiologically active compounds to suppress the host immunity including host inflammatory reaction. however, the specific anti-inflammatory components in mosquitoes remain unknown.201830107813
optimization of cationic (q)-paper for detection of arboviruses in infected mosquitoes.previously (glushakova et al. 2017), a cellulose-based cationic (q) paper derivatized with quaternary ammonium groups was shown to be a convenient platform to collect, preserve, and store nucleic acids (nas) derived from mosquito vectors infected with pathogens for surveillance. nas bind electrostatically to q-paper, but the quantity of na bound depends on the paper's binding capacity. to optimize the original technology for mosquito surveillance, factors that affected na absorbance on q-paper w ...201830099053
discovery and high prevalence of phasi charoen-like virus in field-captured aedes aegypti in south china.arboviruses have caused significant global health concerns during the past decade. in this regard, continuous viral surveillance is essential to timely identify emerging arboviruses and other novel viruses. here, a novel isolate of phasi charoen-like virus (pclv zhanjiang01) was identified from field-captured aedes aegypti mosquitoes in zhanjiang by next generation sequencing. phylogenetic analysis suggested that pclv zhanjiang01 belonged to the genus phasivirus in the family phenuiviridae. the ...201830077072
managing aedes aegypti populations in the first zika transmission zones in the continental united states.the african zika virus swept across the pacific, reaching the new world in 2014. in july, 2016, miami-dade county, florida became the locus of the first mosquito-borne zika transmission zones in the continental united states. control efforts were guided by the centers for disease control and prevention, including aerial and truck sprays of adulticides and larvicides. to improve our understanding of how best to fight zika transmission in an urban environment in the developed world, trap counts of ...201830075097
genetic characterization of chikungunya viruses isolated during the 2015-2017 outbreaks in different states of india, based on their e1 and e2 genes.during 2015-2017, chikungunya virus (chikv) showed a resurgence in several parts of india with karnataka, maharashtra and new delhi accounting for a majority of the cases. e2-e1 gene based characterization revealed indian subcontinent sublineage strains possessing aedes aegypti mosquito-adaptive mutations e1: k211e and e2:v264a, with the 211 site positively selected. novel mutational sites e1: k16e/q, e1: k132q/t, e1: s355t, e2: c19r and e2:s185y could be associated with epitopes or virulence de ...201830073419
a low-cost, battery-powered acoustic trap for surveilling male aedes aegypti during rear-and-release operations.the aedes aegypti mosquito is a primary vector of several serious arboviruses throughout the world and is therefore of great concern to many public health organizations. with vector control methodology pivoting towards rearing and releasing large numbers of genetically modified, sterilized, or wolbachia-infected male mosquitoes to control vector populations, economical surveillance methods for release tracking becomes increasingly necessary. previous work has identified that male ae. aegypti are ...201830071091
rapid evolution and the genomic consequences of selection against interspecific mating.while few species introduced into a new environment become invasive, those that do provide critical information on ecological mechanisms that determine invasions success and the evolutionary responses that follow invasion. aedes albopictus (the asian tiger mosquito) was introduced into the naturalized range of aedes aegypti (the yellow fever mosquito) in the united states in the mid-1980s, resulting in the displacement of a. aegypti in much of the south-eastern united states. the rapid displacem ...201830069966
small females prefer small males: size assortative mating in aedes aegypti mosquitoes.with aedes aegypti mosquitoes now being released in field programmes aimed at disease suppression, there is interest in identifying factors influencing the mating and invasion success of released mosquitoes. one factor that can increase release success is size: released males may benefit competitively from being larger than their field counterparts. however, there could be a risk in releasing only large males if small field females avoid these males and instead prefer small males. here we invest ...201830068363
a gustatory receptor used for rapid detection of tyrophagus putrescentiae in fungi hosts.the storage mite, tyrophagus putrescentiae, found worldwide in many habitats, is an important pest of edible fungi in china. storage mites are tiny and difficult to observe, especially when they occur in fungi composts. in this study, one gustatory receptor protein (tputgr1) was identified from the transcriptome of t. putrescentiae. phylogenetic analysis of grs families from 10 arthropod species revealed that tputgr1 had high homology with the sccagr1 of sarcoptes scabiei and turtgr1-2 of tetran ...201830061635
juvenile hormone-regulated alternative splicing of the taiman gene primes the ecdysteroid response in adult mosquitoes.juvenile hormone (jh) regulates many aspects of insect development and reproduction. in some processes, jh plays a critical role in defining the action of the steroid hormone 20-hydroxyecdysone (20e). in aedes aegypti mosquitoes, jh prepares newly emerged female adults to become competent to synthesize vitellogenin in response to 20e after blood ingestion. the molecular basis of this competence is still not well understood. here, we report that jh regulates pre-mrna splicing of the taiman gene, ...201830061397
comparing the effectiveness of different strains of wolbachia for controlling chikungunya, dengue fever, and zika.once aedes aegypti and aedes albopictus mosquitoes that spread chikungunya virus, dengue virus, and zika virus are infected with wolbachia, they have reduced egg laying rates, reduced transmission abilities, and shorter lifespans. since most infected mosquitoes are only infectious in the last few days of their lives, shortening a mosquito's lifespan by a day or two can greatly reduce their abilities to spread mosquito-borne viral diseases, such as chikungunya, dengue fever, and zika. we develope ...201830059498
modeling the importation and local transmission of vector-borne diseases in florida: the case of zika outbreak in 2016.chikungunya, dengue, and zika viruses are all transmitted by aedes aegypti and aedes albopictus mosquito species, had been imported to florida and caused local outbreaks. we propose a deterministic model to study the importation and local transmission of these mosquito-borne diseases. the purpose is to model and mimic the importation of these viruses to florida via travelers, local infections in domestic mosquitoes by imported travelers, and finally non-travel related transmissions to local huma ...201830053386
modifying the biogents sentinel trap to increase the longevity of captured aedes aegypti.aedes aegypti (l.) (diptera: culicidae) is a vector of viruses causing dengue, zika, chikungunya, and yellow fever and subsequently pose a significant global threat to public health. while sampling live mosquitoes is useful for surveillance purposes, most traps targeting aedes kill captured mosquitoes. the biogents sentinel (bgs) trap, the gold standard for capturing ae. aegypti, is one such trap. in our study, we modified the bgs trap to increase the survival of captured ae. aegypti by replacin ...201830053026
experimental study of the susceptibility of a european aedes albopictus strain to dengue virus under a simulated mediterranean temperature regime.dengue virus (denv) has re-emerged in europe driven by the geographic expansion of the mosquito species aedes albopictus and aedes aegypti (diptera: culicidae) and the introduction of the virus by viraemic travellers. in the present study, the vector competence (vc) of ae. albopictus collected in catalonia (northeast spain) was evaluated for two different denv strains, denv-1 and denv-2, the serotypes responsible for all outbreaks of dengue that have occurred in europe. mosquitoes were reared un ...201830051490
larvicidal effectiveness of azolla pinnata against aedes aegypti (diptera: culicidae) with its effects on larval morphology and visualization of behavioural response.dengue is vector-borne diseases with 390 million infections per year extending over 120 countries of the world. aedes aegypti (l.) (diptera: culicidae) is a primary vector for dengue viral infections for humans. current focus on application of natural product against mosquito vectors has been the main priority for research due to its eco-safety. the extensive use of chemical insecticides has led to severe health problems, environmental pollution, toxic hazards to human and nontarget species, and ...201830050688
the impact of the age of first blood meal and zika virus infection on aedes aegypti egg production and longevity.the impact of senescence and pathogen infection on aedes aegypti life-history traits remains poorly understood. this laboratory study focused on the impact of zika virus (zikv) infection and the age of first blood intake on blood meal and clutch sizes, and more importantly on the egg production ratio per μl of blood. three groups of zikv-infected and uninfected ae. aegypti females that received their first blood meal at 7 (young feeders), 14 (mature feeders) and 21 days old (old feeders) were mo ...201830048481
characterization of dengue virus in aedes aegypti and aedes albopictus spp. of mosquitoes: a study in khyber pakhtunkhwa, pakistan.dengue is a vector-borne disease caused by dengue virus. according to the recent report of cdc that one-third population of the world are at high risk with dengue fever. the prevalence of the dengue hemorrhagic fever was found more in tropical and sub-tropical regions of the world. aedes mosquitoes was reported as the main cause of transmission of dengue virus. so the current study was planned to characterize the virus in aedes mosquitoes collected from different area of pakistan. in current inv ...201830046621
specific human antibody responses to aedes aegypti and aedes polynesiensis saliva: a new epidemiological tool to assess human exposure to disease vectors in the pacific.aedes mosquitoes severely affect the health and wellbeing of human populations by transmitting infectious diseases. in french polynesia, aedes aegypti is the main vector of dengue, chikungunya and zika, and aedes polynesiensis the primary vector of bancroftian filariasis and a secondary vector of arboviruses. tools for assessing the risk of disease transmission or for measuring the efficacy of vector control programmes are scarce. a promising approach to quantify the human-vector contact relies ...201830040826
is autumn the key for dengue epidemics in non endemic regions? the case of argentina.dengue is a major and rapidly increasing public health problem. in argentina, the southern extreme of its distribution in the americas, epidemic transmission takes place during the warm season. since its re-emergence in 1998 two major outbreaks have occurred, the biggest during 2016. to identify the environmental factors that trigger epidemic events, we analyzed the occurrence and magnitude of dengue outbreaks in time and space at different scales in association with climatic, geographic and dem ...201830038860
the effect of skitosnack, an artificial blood meal replacement, on aedes aegypti life history traits and gut microbiota.public health research and vector control frequently require the rearing of large numbers of vector mosquitoes. all target vector mosquito species are anautogenous, meaning that females require vertebrate blood for egg production. vertebrate blood, however, is costly, with a short shelf life. to overcome these constraints, we have developed skitosnack, an artificial blood meal replacement for the mosquito aedes aegypti, the vector of dengue, zika and chikungunya virus. skitosnack contains bovine ...201830038361
classification of containers with aedes aegypti pupae using a neural networks model.this paper discusses the presence of aedes aegypti pupae in different types of containers considering: volume, ph of the container, among other variables.201830036370
acute inhalation toxicity study of n-heneicosane and its combination with diflubenzuron: an attracticide of aedes aegypti.combination of an oviposition pheromone and an insect growth regulator for the control of vectors is an effective approach. there is a need for toxicological evaluation before its introduction. the present study evaluates the acute inhalation toxicity of n-heneicosane and its combination with diflubenzuron in a head-only inhalation exposure chamber made of glass.201830033812
cgti, a novel thermostable kunitz trypsin-inhibitor purified from cassia grandis seeds: purification, characterization and termiticidal activity.cassia grandis trypsin inhibitor (cgti) is a novel plant serine proteinase inhibitor. this study sets out to purify a thermostable inhibitor from the seeds of cassia grandis and to provide biochemical information about a novel peptide belonging to the kunitz family. moreover, toxicity assays against artemia, aedes aegypti larvae-l4 and nasutitermes corniger are evaluated. the purification process was performed using acetone precipitation, trypsin-sepharose-cl4b and superdex-g75. the inhibitor sh ...201830030073
modelling the distribution of the vector aedes aegypti in a central argentine city.aedes aegypti (diptera: culicidae) is an urban mosquito involved in the transmission of numerous viruses, including dengue, chikungunya and zika. in argentina, ae. aegypti is the main vector of dengue virus and has been involved in several outbreaks in regions ranging from northern to central argentina since 2009. in order to evaluate areas of potential vector-borne disease transmission in the city of córdoba, argentina, the present study aimed to identify the environmental, socioeconomic and de ...201830027565
assessment of insecticide resistance in primary dengue vector, aedes aegypti (linn.) from northern districts of west bengal, india.aedes mosquitoes are the major vectors transmitting several arboviral diseases such as dengue, zika and chikungunya worldwide. northern districts of west bengal is home to several epidemics vectored by mosquito including dengue infections, proper control of which depends on efficient vector control. however the onset of insecticide resistance has resulted in failure of vector control approaches. this study was carried out to unveil the level of insecticide resistance prevailing among the primary ...201830026024
copy number variation (cnv) and insecticide resistance in mosquitoes: evolving knowledge or an evolving problem?copy number variation (cnv) in insect genomes is a rich source of potentially adaptive polymorphism which may help overcome the constraints of purifying selection on conserved genes and/or permit elevated transcription. classic studies of amplified esterases and acetylcholinesterase duplication in culex pipiens quantified evolutionary dynamics of cnv driven by insecticidal selection. a more complex and potentially medically impactful form of cnv is found in anopheles gambiae, with both heterogen ...201830025639
melanization of dirofilaria immitis larvae in different culicid species.dirofilaria immitis is an important filarioid transmitted by culicids. the vector role of these arthropods may be influenced by biological events as melanization against d. immitis larvae. this study aimed to detect the occurrence of melanization in culex quinquefasciatus, aedes (stegomyia) aegypti and aedes (stegomyia) albopictus experimentally infected with d. immitis.201830018997
the use of insecticide-treated curtains for control of aedes aegypti and dengue virus transmission in "fraccionamiento" style houses in méxico.dengue, chikungunya, yellow fever, and zika viruses transmitted by aedes aegypti mosquitoes are major public health threats in the tropical and subtropical world. in méxico, construction of large tracts of "fraccionamientos" high density housing to accommodate population growth and urbanization has provided fertile ground for ae. aegypti-transmitted viruses. we investigated the utility of pyrethroid-treated window curtains to reduce both the abundance of ae. aegypti and to prevent dengue virus ( ...201830018645
zika virus outbreak in the pacific: vector competence of regional vectors.in 2013, zika virus (zikv) emerged in french polynesia and spread through the pacific region between 2013 and 2017. several potential aedes mosquitoes may have contributed to the zikv transmission including aedes aegypti, the main arbovirus vector in the region, and aedes polynesiensis, vector of lymphatic filariasis and secondary vector of dengue virus. the aim of this study was to analyze the ability of these two pacific vectors to transmit zikv at a regional scale, through the evaluation and ...201830016372
molecular characterization of the cry gene profile of bacillus thuringiensis isolated from a caribbean region of colombia.in order to characterize native strains of bacillus thuringiensis of the colombian caribbean with toxic effect against insect vectors, 28 samples of bacteria identified as b. thuringiensis were isolated from different soils and muds around the city of valledupar. using a biological test, five isolates of b. thuringiensis showed toxic effect against larvae of aedes aegypti. pcr methods were used to detect cry1, cry2, cry4b, cry10 and cyt1 genes. cry1 and cry2 genes were detected in 35.7% and 32.1 ...201830015421
evidence of vertical transmission of zika virus in field-collected eggs of aedes aegypti in the brazilian amazon.arboviruses are viruses transmitted to humans and other animals by the bite of hematophagous arthropods. infections caused by chikungunya virus (chikv), dengue virus (denv), zika virus (zikv), and the deadlier yellow fever virus (yfv) are current public health problems in several countries, mainly those located in tropical and subtropical regions. one of the main prevention strategies continues to be vector control, with the elimination of breeding sites and surveillance of infested areas. the u ...201830011278
transmission potential of mayaro virus in florida aedes aegypti and aedes albopictus mosquitoes.mayaro virus (mayv) is an emerging mosquito-borne arbovirus present in central and south america that causes arthralgia and febrile illness. domestic mosquitoes aedes aegypti (diptera: culicidae) and aedes albopictus are potential vectors of mayv that may allow for transmission to humans in urban settings. the present paper assesses susceptibility to infection, disseminated infection and transmission potential in florida ae. aegypti and ae. albopictus for mayv. oral infection was significantly h ...201830006976
the occurrence, diversity and blood feeding patterns of potential vectors of dengue and yellow fever in kacheliba, west pokot county, kenya.yellow fever (yf) and dengue (den) viruses are important re-emerging mosquito-borne viruses sharing similar vectors and reservoirs. the last documented yf outbreak in kenya occurred in 1992-95. however, yf virus is re-emerging in bordering countries including uganda, ethiopia and south sudan with the potential for spread to the neighboring regions in kenya. dengue is endemic in kenya with outbreaks being detected in various towns in the north and the coast. this study reports on the aedes (stego ...201830006028
synergy in the adulticidal efficacy of essential oils for the improvement of permethrin toxicity against aedes aegypti l. (diptera: culicidae).in a previous screening program for mosquitocides from local edible plants in thailand, essential oils (eos) of cyperus rotundus, alpinia galanga and cinnamomum verum, were found to possess promising adulticidal activity against aedes aegypti. with the aim of reducing usage of conventional insecticides and improving the management of resistant mosquito populations, this study was designed to determine the potential synergism in the adulticidal efficacy of eos on permethrin toxicity against ae. a ...201830005688
epidemiological significance of dengue virus genetic variation in mosquito infection dynamics.the kinetics of arthropod-borne virus (arbovirus) transmission by their vectors have long been recognized as a powerful determinant of arbovirus epidemiology. the time interval between virus acquisition and transmission by the vector, termed extrinsic incubation period (eip), combines with vector mortality rate and vector competence to determine the proportion of infected vectors that eventually become infectious. however, the dynamic nature of this process, and the amount of natural variation i ...201830005085
the c-type lectin domain gene family in aedes aegypti and their role in arbovirus infection.several medically important flaviviruses that are transmitted by mosquitoes have been shown to bind to the c-type lectin fold that is present in either vertebrate or invertebrate proteins. while in some cases this interaction is part of a neutralizing anti-viral immune response, many reports have implicated this as critical for successful virus entry. despite the establishment of mosquito c-type lectin domain containing proteins (ctldcps) as known host factors in assisting the infectious process ...201830002303
identification of aedes aegypti cis-regulatory elements that promote gene expression in olfactory receptor neurons of distantly related dipteran insects.sophisticated tools for manipulation of gene expression in select neurons, including neurons that regulate sexually dimorphic behaviors, are increasingly available for analysis of genetic model organisms. however, we lack comparable genetic tools for analysis of non-model organisms, including aedes aegypti, a vector mosquito which displays sexually dimorphic behaviors that contribute to pathogen transmission. formaldehyde-assisted isolation of regulatory elements followed by sequencing (faire-se ...201829996889
tropomyosins in mosquito and house dust mite cross-react at the humoral and cellular level.aedes aegypti and dermatophagoides pteronyssinus contain important allergens including cross-reactive tropomyosins. however, the functional and clinical relevance of their cross-reactivity is still debated.201829992665
mosquito arbovirus survey in selected areas of kenya: detection of insect-specific virus.many arboviral outbreaks have occurred in various locations in kenya. entomological surveys are suitable methods for revealing information about circulating arboviruses before human outbreaks are recognized. therefore, mosquitoes were collected in kenya to determine the distribution of arboviruses.201829991925
environmentally-friendly green approach for the production of zinc oxide nanoparticles and their anti-fungal, ovicidal, and larvicidal properties.green synthesis of nanoparticles can be an important alternative compared to conventional physio-chemical synthesis. we utilized scadoxus multiflorus leaf powder aqueous extract as a capping and stabilizing agent for the synthesis of pure zinc oxide nanoparticles (zno nps). further, the synthesized zno nps were subjected to various characterization techniques. transmission electron microscope (tem) analysis showed an irregular spherical shape, with an average particle size of 31 ± 2 nm. furtherm ...201829986435
molecular aspects of the dengue virus infection process: a review.at the present time, dengue is one of the most important arboviruses affecting man, becoming a serious global public health problem, especially in subtropical and tropical countries, where environmental conditions favor the development and proliferation of the mosquito aedes aegypti. dengue is caused by a type of flavivírus, which is an enveloped virus of spherical geometry. nowadays, it is one of the diseases with the highest incidence in brazil, reaching the population of all states, regardles ...201829984641
aedes aegypti in the black sea: recent introduction or ancient remnant?the yellow fever mosquito aedes aegypti transmits viral diseases that have plagued humans for centuries. its ancestral home are forests of africa and ~400-600 years ago it invaded the new world and later europe and asia, causing some of the largest epidemics in human history. the species was rarely detected in countries surrounding the mediterranean sea after the 1950s, but during the last 16 years it re-appeared in madeira, russia and in the eastern coast of the black sea. we genotyped ae. aegy ...201829980229
west nile virus and other mosquito-borne viruses present in eastern europe.eastern europe (ee) has been severely affected by mosquito-borne viruses (moboviruses). in this review, we summarize the epidemiology of moboviruses, with particular attention to west nile virus (wnv). the study of wnv human cases in ee between 2010 and 2016, revealed that the epidemiology of wnv in ee is complex with the combination of introduction of different wnv strains from lineages 1 and 2, and the establishment of endemic cycles. we found a positive correlation between the risk of wnv re- ...201829979950
both living bacteria and eukaryotes in the mosquito gut promote growth of larvae.we recently reported that larval stage aedes aegypti and several other species of mosquitoes grow when living bacteria are present in the gut but do not grow when living bacteria are absent. we further reported that living bacteria induce a hypoxia signal in the gut, which activates hypoxia-induced transcription factors and other processes larvae require for growth. in this study we assessed whether other types of organisms induce mosquito larvae to grow and asked if the density of non-living mi ...201829979680
variation in temephos resistance in field populations of aedes aegypti (diptera: culicidae) in the state of sergipe, northeast brazil.the organophosphate temephos was first used in brazil in the 1960s for the control of aedes aegypti. because of its extensive and longstanding use worldwide, selection for mosquito populations resistant to the chemical has been observed not only in different regions of brazil but also in several parts of the world. the objective of this study was to evaluate the susceptibility of ae. aegypti to the organophosphate temephos, a larvicide used in vector control activities in sergipe/northeast brazi ...201829972557
zika virus: - a review of the main aspects of this type of arbovirosis.the objective of this study was to integrate findings related to zika virus from the scientific literature. an integrative review of medline was conducted, and data search was performed using the health sciences descriptor zika virus and the following filters: full texts available; studies in humans; full articles; and publications in portuguese, french, english, and spanish. after application of the filters, followed by reading of the titles, abstracts, and full texts, 44 studies were included ...201829972554
the effect of nonrandom mating on wolbachia dynamics: implications for population replacement and sterile releases in aedes mosquitoes.wolbachia bacteria are known to cause deviations from random mating and affect sperm competition (sc) in some of their arthropod hosts. because these effects could influence the effectiveness of wolbachia in mosquito population replacement and suppression programs, we developed a theoretical framework to investigate them and we collected relevant data for the wmel infection in aedes aegypti. using incompatibility patterns as a measure of mating success of infected versus uninfected mosquitoes, w ...201829968550
strain-specific pathogenicity and subversion of phenoloxidase activity in the mosquito aedes aegypti by members of the fungal entomopathogenic genus isaria.development of alternative vector control strategies are becoming more pressing given the rapid evolution of insecticide resistance and the rise of vector borne pathogens affecting public health such as dengue, chikungunya and zika. fungal-based biopesticides are promising alternatives to synthetic insecticides because they are ecofriendly and are highly effective at infecting insects through contact. this study evaluated the susceptibility of the yellow fever mosquito ae. aegypti to a range of ...201829967469
continued susceptibility of the wmel wolbachia infection in aedes aegypti to heat stress following field deployment and selection.aedes aegypti mosquitoes infected with the wmel strain of wolbachia are being deployed to control the spread of arboviruses around the world through blockage of viral transmission. blockage by wolbachia in some scenarios may be affected by the susceptibility of wmel to cyclical heat stress during mosquito larval development. we therefore evaluated the potential to generate a heat-resistant strain of wmel in ae. aegypti through artificial laboratory selection and through exposure to field tempera ...201829966368
variation in competence for zikv transmission by aedes aegypti and aedes albopictus in mexico.zikv is a new addition to the arboviruses circulating in the new world, with more than 1 million cases since its introduction in 2015. a growing number of studies have reported vector competence (vc) of aedes mosquitoes from several areas of the world for zikv transmission. some studies have used new world mosquitoes from disparate regions and concluded that these have a variable but relatively low competence for the asian lineage of zikv.201829965958
chikungunya in children: a clinical review.chikungunya (chikv) is an emerging arboviral infection with recent spikes in transmission in the americas. chikungunya is most commonly transmitted by mosquitos, specifically aedes aegypti and aedes albopictus. these mosquitoes are found throughout many parts of the united states. the classic tetrad of symptoms for chikv is fever, symmetric polyarthralgia, maculopapular rash, and nonpurulent conjunctivitis. although the majority (3 of 4) of infected people will be symptomatic, the viral illness ...201829965819
adventitious viruses persistently infect three commonly used mosquito cell lines.mosquito cell lines have been used extensively in research to isolate and propagate arthropod-borne viruses and understand virus-vector interactions. despite their utility as an in vitro tool, these cell lines are poorly defined and may harbor insect-specific viruses. accordingly, we screened four commonly-used mosquito cell lines, c6/36 and u4.4 cells from aedes albopictus, aag2 cells from aedes aegypti, and hsu cells from culex quinquefasciatus, for the presence of adventitious (i.e. exogenous ...201829957338
patterns of occurrence of dengue and chikungunya, and spatial distribution of mosquito vector aedes albopictus in swabi district, pakistan.two principal vector species, aedes aegypti and aedes albopictus, are known for transmission of dengue (den) and chikungunya (chk) in pakistan. we aimed to investigate their spatial and temporal distribution.201829956428
empirical optimization of risk thresholds for dengue: an approach towards entomological management of aedes mosquitoes based on larval indices in the kandy district of sri lanka.larval indices such as premise index (pi), breteau index (bi) and container index (ci) are widely used to interpret the density of dengue vectors in surveillance programmes. these indices may be useful for forecasting disease outbreaks in an area. however, use of the values of these indices as alarm signals is rarely considered in control programmes. therefore, the current study aims to propose threshold values for vector indices based on an empirical modeling approach for the kandy district of ...201829954443
comparison of chikungunya viruses generated using infectious clone or the infectious subgenomic amplicons (isa) method in aedes mosquitoes.reverse genetics systems provide the opportunity to manipulate viral genomes and have been widely used to study rna viruses and to develop new antiviral compounds and vaccine strategies. the recently described method called isa (infectious subgenomic amplicons) gives the possibility to rescue rna viruses in days. we demonstrated in cell culture that the use of the isa method led to a higher genetic diversity of viral populations than that observed using infectious clone technology. however, no r ...201829953474
aedes anphevirus: an insect-specific virus distributed worldwide in aedes aegypti mosquitoes that has complex interplays with wolbachia and dengue virus infection in cells.insect-specific viruses (isvs) of the yellow fever mosquito aedes aegypti have been demonstrated to modulate transmission of arboviruses such as dengue virus (denv) and west nile virus by the mosquito. the diversity and composition of the virome of a. aegypti, however, remains poorly understood. in this study, we characterized aedes anphevirus (aeav), a negative-sense rna virus from the order mononegavirales aeav identified from aedes cell lines was infectious to both a. aegypti and aedes albopi ...201829950416
arthropod evs mediate dengue virus transmission through interaction with a tetraspanin domain containing glycoprotein tsp29fb.dengue virus (denv) is a mosquito-borne flavivirus that causes dengue fever in humans, worldwide. using in vitro cell lines derived from aedes albopictus and aedes aegypti, the primary vectors of denv, we report that denv2/denv3-infected cells secrete extracellular vesicles (evs), including exosomes, containing infectious viral rna and proteins. a full-length denv2 genome, detected in arthropod evs, was infectious to naïve mosquito and mammalian cells, including human-skin keratinocytes and bloo ...201829946031
model-based analysis of experimental data from interconnected, row-configured huts elucidates multifaceted effects of a volatile chemical on aedes aegypti mosquitoes.insecticides used against aedes aegypti and other disease vectors can elicit a multitude of dose-dependent effects on behavioral and bionomic traits. estimating the potential epidemiological impact of a product requires thorough understanding of these effects and their interplay at different dosages. volatile spatial repellent (sr) products come with an additional layer of complexity due to the potential for altered movement of affected mosquitoes and diffusion of volatile particles of the produ ...201829941038
wolbachia enhances insect-specific flavivirus infection in aedes aegypti mosquitoes.mosquitoes transmit a diverse group of human flaviviruses including west nile, dengue, yellow fever, and zika viruses. mosquitoes are also naturally infected with insect-specific flaviviruses (isfs), a subgroup of the family not capable of infecting vertebrates. although isfs are not medically important, they are capable of altering the mosquito's susceptibility to flaviviruses and may alter host fitness. wolbachia is an endosymbiotic bacterium of insects that when present in mosquitoes limits t ...201829938064
using barcoded zika virus to assess virus population structure in vitro and in aedes aegypti mosquitoes.arboviruses such as zika virus (zikv, flaviviridae; flavivirus) must replicate in both mammalian and insect hosts possessing strong immune defenses. accordingly, transmission between and replication within hosts involves genetic bottlenecks, during which viral population size and genetic diversity may be significantly reduced. to help quantify these bottlenecks and their effects, we constructed 4 "barcoded" zikv populations that theoretically contain thousands of barcodes each. after identifying ...201829935423
circulation of chikungunya virus in aedes aegypti in maranhão, northeast brazil.the simultaneous circulation of dengue virus (denv), chikungunya virus (chikv) and zika virus (zikv) arboviruses have placed brazil among the main worldwide endemic areas. brazilian northeast region concentrates the highest incidence of infections caused by chikv and zikv. in maranhão, the second biggest northeastern state, there are cases of human infections caused by these three arboviruses and presence of aedes aegypti and aedes albopictus vectors. in this context, this study aimed to investi ...201829932932
ancient treatment for lice: a source of suggestions for carriers of other infectious diseases?louse infestation is one of the oldest contagious pestilential diseases of humankind, which has recently re-emerged in several developing countries as well as in homeless individuals and migrants. the present work provides the first phase of an historical excursus of louse remedies based on the classics of pharmaceutical literature, codes, pharmacopoeia and treatises. the second phase involves a literature search, based on the principal medical databases (scifinder, pubmed, google scholar, isi-w ...201829932096
evaluation of aedes aegypti (diptera: culicidae) life table attributes upon chikungunya virus replication reveals impact on egg-laying pathways.arthropod-borne virus disease cycles constitute interactions among three primary players-the host, the vector, and the virus-in which the virus needs to interact with the host and the vector to establish its survival. while in the host, some arboviruses replicate aggressively, resulting in host pathogenicity, and manifest as a disease condition. these viruses more often utilize the vectors as reservoirs before they are transmitted to the host and therefore do not amplify to as large titers as th ...201829931258
a high-risk zika and dengue transmission hub: virus detections in mosquitoes at a brazilian university campus.zika virus (zikv) and dengue virus (denv) are mosquito-borne flaviviruses prevalent throughout tropical regions. currently, management of zikv and denv centers on control of the primary vector aedes aegypti. this vector is highly anthropophilic and is therefore prevalent throughout densely urbanised landscapes. a new passive trap for gravid ae. aegypti (gravid aedes trap - gat) was developed for mosquito surveillance. here the different killing agents and the level of transmission of arboviruses ...201829929561
risk factors for the presence of dengue vector mosquitoes, and determinants of their prevalence and larval site selection in dhaka, bangladesh.dengue viruses are responsible for over 100 million infections a year worldwide and are a public health concern in bangladesh. although risk of transmission is high, data on vector population characteristics are scanty in bangladesh; therefore, a comprehensive prediction of the patterns of local virus transmission is not possible. recognizing these gaps, multi-year entomological surveys were carried out in dhaka, where the disease is most frequently reported. the specific objectives of the prese ...201829928055
vector competence of aedes aegypti, culex tarsalis, and culex quinquefasciatus from california for zika virus.zika virus (zikv) has emerged since 2013 as a significant global human health threat following outbreaks in the pacific islands and rapid spread throughout south and central america. severe congenital and neurological sequelae have been linked to zikv infections. assessing the ability of common mosquito species to transmit zikv and characterizing variation in mosquito transmission of different zikv strains is important for estimating regional outbreak potential and for prioritizing local mosquit ...201829927940
hydrophobic nanoprecipitates of β-cyclodextrin/avermectins inclusion compounds reveal insecticide activity against aedes aegypti larvae and low toxicity against fibroblasts.in the present work, hydrophobic nanoprecipitates (hnps) of inclusion complexes formed between β-cyclodextrin (βcd) and the avermectins (avms) named eprinomectin (epri) and ivermectin (iver) were synthesized and characterized, and their larvicidal activity against aedes aegypti and human safety against fibroblasts were evaluated. initially, thermogravimetric analysis/differential thermal analysis data revealed that inclusion increased the thermal stability of avms in the presence of βcd. nuclear ...201829925239
field testing & evaluation of the efficacy & duration of effectiveness of a biolarvicide, bactivec® sc (bacillus thuringiensis var. israelensis sh-14) in bengaluru, india.different formulations of bacillus thuringiensis var. israelensis (bti) have been tested against different mosquito vectors and other insects for their residual activity. in the present study, the efficacy and residual activity of a new formulation of bti (bactivec suspension concentrate) were evaluated against immature stages of anopheles stephensi liston (diptera: culicidae), aedes aegypti linnaeus (diptera: culicidae) and culex quinquefasciatus say (diptera: culicidae), in natural habitats in ...201829923520
development of an urban molecular xenomonitoring system for lymphatic filariasis in the recife metropolitan region, brazil.molecular xenomonitoring (mx)-pathogen detection in the mosquito rather than human-is a promising tool for lymphatic filariasis (lf) surveillance. in the recife metropolitan region (rmr), the last lf focus in brazil, culex quinquefasciatus mosquitoes have been implicated in transmitting wuchereria bancrofti parasites. this paper presents findings on the ideal mosquito collection method, mosquito dispersion, w. bancrofti infection in mosquitoes and w. bancrofti antigen in humans to aid mx develop ...201830325933
response to comments on qureshi et al (j pak med assoc 2017; 67: 1797-1802) seasonal and spatial quantitative changes in aedes aegypti under distinctly different ecological areas of lahore, pakistan. 201830323379
comments on qureshi et al (j pak med assoc 2017; 67: 1797-1802) - seasonal and spatial quantitative changes in aedes aegypti under distinctly different ecological areas of lahore, pakistan. 201830323378
location, seasonal, and functional characteristics of water holding containers with juvenile and pupal aedes aegypti in southern taiwan: a cross-sectional study using hurdle model analyses.aedes aegypti carries several viruses of public health importance, including the dengue virus. dengue is the most rapidly spreading mosquito-borne viral disease in the world. prevention and control of dengue mainly rely on mosquito control as there is no antiviral treatment or a who-approved vaccine. to reduce the ae. aegypti population, studying the characteristics of their habitats is necessary. aedes aegypti prefer breeding in artificial water holding containers in peridomestic or domestic se ...201830321168
effects of alternative blood sources on wolbachia infected aedes aegypti females within and across generations.wolbachia bacteria have been identified as a tool for reducing the transmission of arboviruses transmitted by aedes aegypti. research groups around the world are now mass rearing wolbachia-infected ae. aegypti for deliberate release. we investigated the fitness impact of a crucial element of mass rearing: the blood meal required by female ae. aegypti to lay eggs. although ae. aegypti almost exclusively feed on human blood, it is often difficult to use human blood in disease-endemic settings. whe ...201830314399
genetic variation in insect vectors: death of typology?the issue of typological versus population thinking in biology is briefly introduced and defined. it is then emphasized how population thinking is most relevant and useful in vector biology. three points are made: (1) vectors, as they exist in nature, are genetically very heterogeneous. (2) four examples of how this is relevant in vector biology research are presented: understanding variation in vector competence, gwas, identifying the origin of new introductions of invasive species, and resista ...201830314367
zika virus: a critical analysis and pharmaceutical perspectives.zika virus belongs to family of viruses 'flaviviridae' and spreads mostly by daytime-active aedes mosquitoes. the resulting infection is known as zika fever. it is usually asymptomatic or often causes mild symptoms, which are very similar to dengue fever. the aedes aegypti mosquito is also responsible for dengue and chikungunya viruses. zika virus can spread by crossing the placental barrier from a pregnant mother to a fetus, which can result in microcephaly, severe brain malformations including ...201830311585
human and entomologic investigations of chikungunya outbreak in mandera, northeastern kenya, 2016.chikungunya is a reemerging vector borne pathogen associated with severe morbidity in affected populations. lamu, along the kenyan coast was affected by a major chikungunya outbreak in 2004. twelve years later, we report on entomologic investigations and laboratory confirmed chikungunya cases in northeastern kenya. patient blood samples were received at the kenya medical research institute (kemri) viral hemorrhagic fever laboratory and the immunoglobulin m enzyme linked immunosorbent assay (igm ...201830308064
spatio-temporal distribution of vertically transmitted dengue viruses by aedes aegypti (diptera: culicidae) from arroyo naranjo, havana, cuba.to study the distribution of vertical transmission of dengue viruses in field-collected aedes aegypti larvae in the municipality of arroyo naranjo in havana, cuba.201830307674
evidence that rh proteins in the anal papillae of the freshwater mosquito aedes aegypti are involved in the regulation of acid-base balance in elevated salt and ammonia environments.aedes aegypti commonly inhabit ammonia-rich sewage effluents in tropical regions of the world where the adults are responsible for the spread of disease. studies have shown the importance of the anal papillae of a. aegypti in ion uptake and ammonia excretion. the anal papillae express ammonia transporters and rhesus (rh) proteins which are involved in ammonia excretion and studies have primarily focused on understanding these mechanisms in freshwater. in this study, effects of rearing larvae in ...201830305376
the effect of parasite infection on the recombination rate of the mosquito aedes aegypti.sexual reproduction and meiotic recombination generate new genetic combinations and may thereby help an individual infected by a parasite to protect its offspring from being infected. while this idea is often used to understand the evolutionary forces underlying the maintenance of sex and recombination, it also suggests that infected individuals should increase plastically their rate of recombination. we tested the latter idea with the mosquito aedes aegypti and asked whether females infected by ...201830300349
quantification of permethrin resistance and kdr alleles in florida strains of aedes aegypti (l.) and aedes albopictus (skuse).recent outbreaks of locally transmitted dengue and zika viruses in florida have placed more emphasis on integrated vector management plans for aedes aegypti (l.) and aedes albopictus skuse. adulticiding, primarily with pyrethroids, is often employed for the immediate control of potentially arbovirus-infected mosquitoes during outbreak situations. while pyrethroid resistance is common in ae. aegypti worldwide and testing is recommended by cdc and who, resistance to this class of products has not ...201830356237
an unusual occurrence of stromal keratitis in dengue fever.dengue is a mosquito-borne infection endemic in the tropical and subtropical regions of the world. classic dengue fever is a self-limiting, influenza-like illness transmitted by aedes aegypti mosquito. ophthalmic manifestations though rare can involve both the anterior and posterior segments and are usually associated with the thrombocytopenic state. however, ophthalmic complications such as anterior uveitis and vasculitis suggest immune-mediated pathogenesis. herein, we report a rare case of st ...201830355887
validation of serum aminotransferases levels to define severe dengue fever in children.we aimed to study the pattern of liver-injury in children with dengue fever (df) and validate serum aminotransferase ≥1,000 iu/l as a marker of severe df.201830345242
the sequence of a male-specific genome region containing the sex determination switch in aedes aegypti.aedes aegypti is the principal vector of several important arboviruses. among the methods of vector control to limit transmission of disease are genetic strategies that involve the release of sterile or genetically modified non-biting males, which has generated interest in manipulating mosquito sex ratios. sex determination in ae. aegypti is controlled by a non-recombining y chromosome-like region called the m locus, yet characterisation of this locus has been thwarted by the repetitive nature o ...201830342535
ultrastructural analysis of chikungunya virus dissemination from the midgut of the yellow fever mosquito, aedes aegypti.the transmission cycle of chikungunya virus (chikv) requires that mosquito vectors get persistently infected with the virus, following its oral acqsuisition from a vertebrate host. the mosquito midgut is the initial organ that gets infected with orally acquired chikv. following its replication in the midgut epithelium, the virus exits the midgut and infects secondary tissues including the salivary glands before being transmitted to another host. here, we investigate the pattern of chikv dissemin ...201830340365
the transcriptional response of aedes aegypti with variable extrinsic incubation periods for dengue virus.dengue fever is the most prevalent arboviral disease globally. dengue virus is transmitted primarily by the aedes aegypti mosquito. one measure of the mosquito's efficiency as a vector is the extrinsic incubation period (eip), which is the time between the ingestion of viremic blood and the emergence of virions in the saliva. the longer it takes virus to infect the midgut and traverse to the saliva, the fewer opportunities the mosquito will have to transmit the pathogen over its lifetime. we hav ...201830335126
zikv demonstrates minimal pathologic effects and mosquito infectivity in viremic cynomolgus macaques.to evaluate the effects of zikv infection on non-human primates (nhps), as well as to investigate whether these nhps develop sufficient viremia to infect the major urban vector mosquito, aedes aegypti, four cynomolgus macaques (macaca fascicularis) were subcutaneously infected with 5.0 log10 focus-forming units (ffu) of dna clone-derived zikv strain fss13025 (asian lineage, cambodia, 2010). following infection, the animals were sampled (blood, urine, tears, and saliva), underwent daily health mo ...201830469417
neuronal responses of antennal olfactory sensilla to insect chemical repellents in the yellow fever mosquito, aedes aegypti.the yellow fever mosquito, aedes aegypti, is a vector of many human diseases such as yellow fever, dengue fever, and zika. as insecticide resistance has been widely reported, chemical repellents have been adopted as alternative options for mosquito and mosquito-borne disease control. this study characterized the responses of olfactory receptor neurons (orns) in different types of antennal olfactory sensilla in ae. aegypti to 48 chemicals that exhibited repellent activity in various insect specie ...201830291492
plant essential oils enhance diverse pyrethroids against multiple strains of mosquitoes and inhibit detoxification enzyme processes.mosquito-borne diseases account for the deaths of approximately 700,000 people annually throughout the world, with many more succumbing to the debilitating side effects associated with these etiologic disease agents. this is exacerbated in many countries where the lack of mosquito control and resources to prevent and treat mosquito-borne disease coincide. as populations of mosquito species grow more resistant to currently utilized control chemistries, the need for new and effective chemical mean ...201830287743
aedes aegypti mosquitoes from guadeloupe (french west indies) are able to transmit yellow fever virus.the recent yellow fever epidemic in brazil has raised the concern of outbreaks in neighboring countries, particularly in the caribbean region where the vector aedes aegypti is predominant. this threat comes from the past when in the americas, this disease caused devastating urban epidemics. we report the vector competence of ae. aegypti from guadeloupe for yellow fever virus by determining different parameters describing virus infection, dissemination, and transmission. the results indicate that ...201830265716
efficacy of mosquito coils: cross-resistance to pyrethroids in aedes aegypti (diptera: culicidae) from indonesia.aedes aegypti (l.) (diptera: culicidae) is the primary vector of several arthropod-borne viral infectious diseases globally. relentless vector control efforts are performed to curtail disease transmissions, insecticides remain as the first line of defense in indonesia. with a dearth of publication on the efficacy of mosquito coil in indonesia, this is the first report related to mosquito coil despite its common use in households. ae. aegypti mosquitoes were sampled from nine regencies in indones ...201830265353
nanosilver crystals capped with bauhinia acuminata phytochemicals as new antimicrobials and mosquito larvicides.to develop novel nanoformulated insecticides and antimicrobials, herein we produced ag nanoparticles (agnps) using the bauhinia acuminata leaf extract. this unexpensive aqueous extract acted as a capping and reducing agent for the formation of agnps. we characterized b. acuminata-synthesized agnps by uv-vis and ftir spectroscopy, xrd and tem analyses. uv-vis spectroscopy analysis of b. acuminata-synthesized agnps showed a peak at 441.5 nm. ftir shed light on functional groups from the phytoconst ...201830262272
high functional conservation of takeout family members in a courtship model system.takeout (to) is one of the male-specific genes expressed in the fat body that regulate male courtship behavior, and has been shown to act as a secreted protein in conjunction with courtship circuits. there are 23 takeout family members in drosophila melanogaster, and homologues of this family are distributed across insect species. sequence conservation among family members is low. here we test the functional conservation of takeout family members by examining whether they can rescue the takeout ...201830261021
history of zikv infections in india and management of disease outbreaks.zika virus (zikv) is an emerging arbovirus infection endemic in multiple countries spread from asia, africa to the americas and europe. previously known to cause rare and fairly benign human infections, zikv has become a major international public health emergency after being linked to unexpected neurological complications, that includes fetal brain damage/death and microcephaly in babies born to infected mothers and guillain-barre syndrome (gbs) in adults. it appears that a single genetic mutat ...201830258421
first report of v1016g and s989p knockdown resistant (kdr) mutations in pyrethroid-resistant sri lankan aedes aegypti mosquitoes.dengue is a serious arboviral disease in sri lanka with a large number of dengue fever (df) cases every year. control of the primary vector aedes aegypti depends upon larval habitat source reduction and insecticide application. however, increases in the number of reported cases suggest the inefficiency of current control strategies and the possibility of resistance to currently used insecticides. early detection of mutations in the voltage-gated sodium channel (vgsc) gene that confer knockdown r ...201830257701
c-type lectin-20 interacts with alp1 receptor to reduce cry toxicity in aedes aegypti.aedes aegypti is a crucial vector for human diseases, such as yellow fever, dengue, chikungunya, and zika viruses. today, a major challenge throughout the globe is the insufficient availability of antiviral drugs and vaccines against arboviruses, and toxins produced by bacillus thuringiensis (bt) are still used as biological agents for mosquito control. the use of cry toxins to kill insects mainly depends on the interaction between cry toxins and important toxin receptors, such as alkaline phosp ...201830257487
differential transmission of asian and african zika virus lineages by aedes aegypti from new caledonia.zika virus (zikv) is a flavivirus that is transmitted to humans by aedes mosquitoes. zikv is divided into two phylogenetic lineages, african and asian. in the asian lineage, pacific and american clades have been linked to the recent worldwide outbreak of zikv. the aim of this study was to measure the vector competence of aedes aegypti for seven zikv strains belonging to both lineages. we demonstrate that ae. aegypti from new caledonia (nc), south pacific region, is a low-competence vector for as ...201830254274
sex and species specific hearing mechanisms in mosquito flagellar ears.hearing is essential for the courtship of one of the major carriers of human disease, the mosquito. males locate females through flight-tone recognition and both sexes engage in mid-air acoustic communications, which can take place within swarms containing thousands of individuals. despite the importance of hearing for mosquitoes, its mechanisms are still largely unclear. we here report a multilevel analysis of auditory function across three disease-transmitting mosquitoes (aedes aegypti, anophe ...201830254270
population structure of a vector of human diseases: aedes aegypti in its ancestral range, africa.aedes aegypti, the major vector of dengue, yellow fever, chikungunya, and zika viruses, remains of great medical and public health concern. there is little doubt that the ancestral home of the species is africa. this mosquito invaded the new world 400-500 years ago and later, asia. however, little is known about the genetic structure and history of ae. aegypti across africa, as well as the possible origin(s) of the new world invasion. here, we use ~17,000 genome-wide single nucleotide polymorphi ...201830250667
microbiome interaction networks and community structure from laboratory-reared and field-collected aedes aegypti, aedes albopictus, and culex quinquefasciatus mosquito vectors.microbial interactions are an underappreciated force in shaping insect microbiome communities. although pairwise patterns of symbiont interactions have been identified, we have a poor understanding regarding the scale and the nature of co-occurrence and co-exclusion interactions within the microbiome. to characterize these patterns in mosquitoes, we sequenced the bacterial microbiome of aedes aegypti, ae. albopictus, and culex quinquefasciatus caught in the field or reared in the laboratory and ...201830250462
microcystin and pyriproxyfen are toxic to early stages of development in rhamdia quelen: an experimental and modelling study.the recent increase of freshwater eutrophication has favored cyanobacteria blooms and consequently the increase of toxins such as microcystin-lr in aquatic environments, but few is know about the associated effect of toxin and other compounds. pyriproxyfen is an insecticide indicated by who (world health organization) to control aedes aegypti mosquito (vector of dengue, chikungunya and zika diseases), however, the effects are not well described to non-target species, such as fish. the early life ...201830278392
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