Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| examination of enterococcus faecalis toxin-antitoxin system toxin fst function utilizing a pheromone-inducible expression vector with tight repression and broad dynamic range. | tools for regulated gene expression in enterococcus faecalis are extremely limited. in this report, we describe the construction of an expression vector for e. faecalis, designated pcie, utilizing the pq pheromone-responsive promoter of plasmid pcf10. we demonstrate that this promoter is tightly repressed, responds to nanogram quantities of the peptide pheromone, and has a large dynamic range. to demonstrate its utility, the promoter was used to control expression of the toxic peptides of two pa ... | 2017 | 28348028 |
| yeasts harbored by vespine wasps in the pacific northwest. | the ecological role of social wasps has been extensively studied, but little is known about symbiotic relationships of these wasps with microbes. recently, it was shown that vespid wasps in europe carry yeasts, predominantly saccharomyces cerevisiae, in their gastrointestinal (gi) tract. interestingly, this niche allowed for sexual recombination of yeasts to occur and the formation of novel hybrid species. our goals were 1) to survey the gi tract of eusocial wasps in the pacific northwest for th ... | 2017 | 28169397 |
| antibacterial effect of azadirachta indica (neem) or curcuma longa (turmeric) against enterococcus faecalis compared with that of 5% sodium hypochlorite or 2% chlorhexidine in vitro. | the purpose of this study was to compare the antibacterial properties of azadirachta indica (neem) or curcuma longa (turmeric) against enterococcus faecalis with those of 5% sodium hypochlorite or 2% chlorhexidine as root canal irrigants in vitro. the activity of neem, chlorhexidine, sodium hypochlorite, or turmeric against e. faecalis was measured on agar plates using the agar diffusion method. the tube dilution method was used to determine the minimum inhibitory concentration (mic) and minimum ... | 2017 | 28724858 |
| biofilm formation in enterococci: genotype-phenotype correlations and inhibition by vancomycin. | enterococci are nosocomial pathogens that can form biofilms, which contribute to their virulence and antibiotic resistance. although many genes involved in biofilm formation have been defined, their distribution among enterococci has not been comprehensively studied on a genome scale, and their diagnostic ability to predict biofilm phenotypes is not fully established. here, we assessed the biofilm-forming ability of 90 enterococcal clinical isolates. major patterns of virulence gene distribution ... | 2017 | 28720810 |
| virulence genes in biofilm producer enterococcus faecalis isolates from root canal infections. | enterococcus faecalis is occurring in opportunistic infections involving the oral cavity. this study aimed to evaluate the presence of e. faecalis virulence genes in dental root canal isolates recovered from advanced chronic periodontitis patients. one hundred e. faecalis isolated from dental root canal during july 2015 to oct 2016. after analysis of biofilm formation by the semi-quantitative determination in 96-well flat bottom polystyrene plates, the presence of asa, esp, efaa, ace, ebpr, gel ... | 2017 | 28719346 |
| screening of oxalate degrading lactic acid bacteria of food origin. | a screening for oxalate degrading abilities was initially carried on within lactic acid bacteria cultures of different food origin. seventy-nine strains were drop-inoculated onto mrs agar plates containing calcium oxalate. by comparing colonies diameters, 31 strains were used to inoculate, in parallel, mrs and mrs modified by sodium oxalate addition. differences in the strains' growth were assessed by colony forming unit counts. for two strains, the growth in oxalate enriched medium was signific ... | 2017 | 28713789 |
| genetic characterisation confirms sporadic occurrence of vancomycin-resistant enterococcus faecalis in copenhagen, denmark. | 2017 | 28709988 | |
| short-term alcohol abstinence improves antibacterial defenses of chronic alcohol-consuming mice against gut bacteria-associated sepsis caused by enterococcus faecalis oral infection. | the effects of short-term alcohol abstinence on host antibacterial resistance against enterococcus faecalis oral infection was investigated in chronic alcohol-consuming mice [mice with 0.1 g/day of 20% ethanol consumption for 12 or 16 weeks (cac-mice)]. these mice were highly susceptible to the infection; however, after 7 days of alcohol abstinence (aacac-mice), their antibacterial resistances were completely restored to the normal mouse level. normal mice inoculated with cac-mouse hepatic macro ... | 2017 | 28708971 |
| virulence and antibiotic resistance of enterococci isolated from healthy breastfed infants. | pathogenic ability has been extensively studied in clinical enterococci, but to a lesser extent in community-derived ones. most studies to date in enterococci from healthy infants have been focused on enterococcus faecalis, despite the growing concern about nosocomial infections caused by e. faecium. in this work, we studied the antibiotic resistance and virulence determinants of 26 e. faecalis and 15 e. faecium intestinal isolates from spanish healthy breastfed infants. overall, commensal enter ... | 2017 | 28708453 |
| evaluation of free or anchored antimicrobial peptides as candidates for the prevention of orthopaedic device-related infections. | the prevention of implant-associated infection, one the most feared complications in orthopaedic surgery, remains a major clinical challenge and urges development of effective methods to prevent bacterial colonization of implanted devices. alpha-helical antimicrobial peptides (amps) may be promising candidates in this respect due to their potent and broad-spectrum antimicrobial activity, their low tendency to elicit resistance and possible retention of efficacy in the immobilized state. the aim ... | 2017 | 28707817 |
| postoperative enterococcus faecalis endophthalmitis: virulence factors leading to poor visual outcome. | purpose postoperative endophthalmitis caused by enterococcus faecalis often results in poor visual outcome due to retinal destruction. we evaluated the virulence factors of enterococcus faecalis clinical isolates and analyzed their relationship with retinal destruction and poor visual outcomes in postoperative bacterial endophthalmitis (pbe) following cataract surgery. | 2017 | 28707018 |
| characterization and application of antilisterial enterocins on model fresh cheese. | enterococcus faecalis strains isolated from an artisanal cheese were selected based on enterocin production against listeria monocytogenes. the strains formed biofilms and presented high hydrophobic character and good autoaggregation and coaggregation capacity with l. monocytogenes. strains l3a21m3 and l3b1k3 presented high survival under gastrointestinal conditions, were able to adhere to human intestinal cells (caco-2 and ht-29), and blocked the adhesion and invasion of l. monocytogenes. the a ... | 2017 | 28703625 |
| restructuring of enterococcus faecalis biofilm architecture in response to antibiotic-induced stress. | bacterial biofilms are intrinsically resistant to antimicrobial treatment, which contributes to microbial persistence in clinical infections. enterococcus faecalis is an opportunistic pathogen that readily forms biofilms and is the most prevalent enterococcal species identified in healthcare-associated infections. since intrinsic resistance to multiple antibiotics is common for enterococci, and antibiotic resistance is elevated in biofilm populations, it is imperative to understand the mechanism ... | 2017 | 28685097 |
| draft genome sequence of enterococcus faecalis atcc baa-2128. | while a part of the native gut microflora, the gram-positive bacterium enterococcus faecalis can lead to serious infections elsewhere in the body. the draft genome of e. faecalis strain atcc baa-2128, isolated from piglet feces, was examined. this draft genome consists of 42 contigs, 12 of which exhibit homology to annotated plasmids. | 2017 | 28684576 |
| role of purified β-1, 3 glucan binding protein (β-gbp) from paratelphusa hydrodromus and their anti-inflammatory, antioxidant and antibiofilm properties. | β- 1, 3-glucan binding protein (β-gbp), a pattern recognition protein (prp), plays a critical role in triggering the innate immune response by detecting β-glucan found on the surface of microbes. in the present study, β-gbp was purified from the haemolymph of rice field crab paratelphusa hydrodromus by affinity column chromatography. the monomeric protein ph-β-gbp appeared as a single band with a molecular weight of approximately 95 kda in sds-page analysis and its purity was determined to be 89 ... | 2017 | 28684323 |
| incidence, clinical characteristics, and outcomes of nosocomial enterococcus spp. bloodstream infections in a tertiary-care hospital in beijing, china: a four-year retrospective study. | enterococcus spp. are the common cause of nosocomial bloodstream infections (bsis) with high morbidity and mortality. the purpose of this study was to characterize the incidence, clinical and microbiological features, and mortality of nosocomial enterococcal bsis at a large chinese tertiary-care hospital in beijing, china. | 2017 | 28680588 |
| the enterococcus faecalis virulence factor elra interacts with the human four-and-a-half lim domains protein 2. | the commensal bacterium enterococcus faecalis is a common cause of nosocomial infections worldwide. the increasing prevalence of multi-antibiotic resistant e. faecalis strains reinforces this public health concern. despite numerous studies highlighting several pathology-related genetic traits, the molecular mechanisms of e. faecalis virulence remain poorly understood. in this work, we studied 23 bacterial proteins that could be considered as virulence factors or involved in the enterococcus inte ... | 2017 | 28676674 |
| stochasticity in the enterococcal sex pheromone response revealed by quantitative analysis of transcription in single cells. | in enterococcus faecalis, sex pheromone-mediated transfer of antibiotic resistance plasmids can occur under unfavorable conditions, for example, when inducing pheromone concentrations are low and inhibiting pheromone concentrations are high. to better understand this paradox, we adapted fluorescence in situ hybridization chain reaction (hcr) methodology for simultaneous quantification of multiple e. faecalis transcripts at the single cell level. we present direct evidence for variability in the ... | 2017 | 28671948 |
| carriage of multidrug resistant enterococcus faecium and enterococcus faecalis among apparently healthy humans. | enterococci are indigenous flora of the gastro-intestinal tracts of animals and humans. recently, interest in two major species, e. faecium and e. faecalis, has heightened because of their ability to cause serious infections and their intrinsic resistance to antimicrobials. this study was aimed at determining the prevalence of e. faecium and e. faecalis in human faecal samples and evaluating the susceptibility of the isolates to antibiotics. | 2017 | 28670644 |
| prevalence and genetic diversity of enterococcus faecalis isolates from mineral water and spring water in china. | enterococcus faecalis is an important opportunistic pathogen which is frequently detected in mineral water and spring water for human consumption and causes human urinary tract infections, endocarditis and neonatal sepsis. the aim of this study was to determine the prevalence, virulence genes, antimicrobial resistance and genetic diversity of e. faecalis from mineral water and spring water in china. of 314 water samples collected from january 2013 to january 2014, 48 samples (15.3%) were contami ... | 2017 | 28670302 |
| determining the optimal dose of 1940-nm thulium fiber laser for assisting the endodontic treatment. | insufficient cleaning, the complex anatomy of the root canal system, inaccessible accessory canals, and inadequate penetration of irrigants through dentinal tubules minimizes the success of the conventional endodontic treatment. laser-assisted endodontic treatment enhances the quality of conventional treatment, but each laser wavelength has its own its own limitations. the optimal parameters for the antibacterial efficiency of a new wavelength, 1940-nm thulium fiber laser, were firstly investiga ... | 2017 | 28669067 |
| urinary tract infections after kidney transplantation: a risk factor analysis of 417 patients. | background the aim of this study was to evaluate the number of urinary tract infections (uti) that occur after kidney transplantation (kt) and to identify possible risk factors for development of a uti. material and methods we retrospectively analyzed all kts performed between january 2012 and december 2013 in the erasmus university medical center, rotterdam. uti was scored if: (1) a patient had a urine culture with no more than 2 species of microorganisms, (2) at least 1 of which was a bacteriu ... | 2017 | 28663538 |
| altered antibacterial activity of curcumin in the presence of serum albumin, plasma and whole blood. | antibacterial effect is one of the major therapeutic activities of plant-derived curcumin. this work evaluated the effect of serum albumin, human plasma, and whole blood on the in vitro activity of curcumin against eight clinical bacterial isolates by standard broth microdilution and plate-counting methods. toxicological effects of curcumin towards human red blood cells (rbcs) and peripheral blood mononuclear cells (pbmcs) were also investigated. curcumin exhibited weak activity against gram-neg ... | 2017 | 28649069 |
| [mitral valve endocarditis diagnosed after panophthalmitis with enterococcus faecalis]. | an 86-year-old man underwent cataract surgery on the left eye, but suddenly he lost his vision three days after surgery. topical treatments for panophthalmitis were started according to international guidelines. later, a broad-range polymerase chain reaction showed enterococcus faecalis in corpus vitreum, and iv antiobiotic treatment was initiated. to exclude endocarditis an echocardiography was performed, and this showed mitral regurgitation with a small mobile vegetation. uncertainty concernin ... | 2017 | 28648168 |
| antibacterial effect of genetically-engineered bacteriophage ϕef11/ϕfl1c(δ36)p(nisa) on dentin infected with antibiotic-resistant enterococcus faecalis. | enterococcus faecalis is a gram-positive facultative anaerobic bacterium, which is present in 30-89% of teeth with postendodontic treatment failures. e. faecalis is capable of penetrating dentinal tubules and surviving as a monoculture after conventional endodontic therapy, indicating that it is resistant to commonly used endodontic disinfection protocols. different e. faecalis strains have shown resistance to several antibiotics, and have been associated with both dental pathology and systemic ... | 2017 | 28646693 |
| phtβ-promoted mobilization of non-conjugative resistance plasmids from enterococcus faecium to enterococcus faecalis. | to analyse the recombination events associated with conjugal mobilization of two multiresistance plasmids, prum17i48 and plag (formerly named pdo1-like), from enterococcus faecium 17i48 to enterococcus faecalis jh2-2. | 2017 | 28645197 |
| methacrylate-based root canal sealer containing chlorexidine and α-tricalcium phosphate. | the aim of this study was to develop and to characterize a methacrylate-based root canal sealer containing chlorhexidine (chx) and α-tricalcium phosphate (α-tcp). experimental dual cure methacrylate-based sealer was produced containing 0, 2.5, or 5 wt% of chx and 0, 25, or 50 wt% of α-tcp. experimental sealers were evaluated based on flow, film thickness, radiopacity, degree of conversion (dc), degradation in water, ph and antibacterial activity. flow ranged from 15.09 ± 0.11 to 17.47 ± 0.42 mm. ... | 2017 | 28644564 |
| exploiting crispr-cas to manipulate enterococcus faecalis populations. | crispr-cas provides a barrier to horizontal gene transfer in prokaryotes. it was previously observed that functional crispr-cas systems are absent from multidrug-resistant (mdr) enterococcus faecalis, which only possess an orphan crispr locus, termed crispr2, lacking cas genes. here, we investigate how the interplay between crispr-cas genome defense and antibiotic selection for mobile genetic elements shapes in vitro e. faecalis populations. we demonstrate that crispr2 can be reactivated for gen ... | 2017 | 28644125 |
| synthesis and antimicrobial activity of chiral quaternary n-spiro ammonium bromides with 3',4'-dihydro-1'h-spiro[isoindoline-2,2'-isoquinoline] skeleton. | a new class of highly functionalized tetrahydroisoquinolines with a quaternary carbon stereocenter was synthesized starting from an easily accessible l-tartaric acid. nine strains of bacteria (staphylococcus aureus, streptococcus pyogenes, streptococcus mutans, streptococcus salivarius, bacillus subtilis, enterococcus faecalis, moraxella catarrhalis, escherichia coli, campylobacter jejuni) were used for the determination of minimal inhibitory concentration (mic) and minimal bactericidal concentr ... | 2017 | 28740363 |
| a prospective randomised trial of isolated pathogens of surgical site infections (ssi). | every surgical wound is colonized by bacteria, but only a small percentage displays symptoms of infection. the distribution of pathogens isolated in surgical site infections has not significantly changed over the last decades. staph. aureus, coag(-) staphylococci, enterococcus spp and e. coli are the main strains appearing. in addition, a continuously rising proportion of surgical site infections caused by resistant bacterial species (mrsa, c. albicans) has been reported. | 2017 | 28761643 |
| implementation of the new virtuo blood culture system: evaluation and comparison to the 3d system using simulated blood cultures. | to evaluate the performances of the newly approved bact/alert virtuo blood culture system for the recovery of bloodstream pathogens and compare it to the bact/alert 3d system. | 2017 | 28583022 |
| comparative antimicrobial efficacy of selected root canal irrigants on commonly isolated microorganisms in endodontic infection. | this study aims to evaluate and compare the antimicrobial efficacy of three selected root canal irrigants (biopure mtad, metronidazole, aztreonam) against microbes commonly isolated from polymicrobial microbiota of root canal infection. | 2017 | 28435359 |
| azathioprine, mercaptopurine, and 5-aminosalicylic acid affect the growth of ibd-associated campylobacter species and other enteric microbes. | campylobacter concisus is a bacterium that is associated with inflammatory bowel disease (ibd). immunosuppressive drugs including azathioprine (aza) and mercaptopurine (mp), and anti-inflammatory drug such as 5-aminosalicylic acid (5-asa) are commonly used to treat patients with ibd. this study aimed to examine the effects of aza, mp, and 5-asa on the growth of ibd-associated bacterial species and to identify bacterial enzymes involved in immunosuppressive drug metabolism. a total of 15 bacteria ... | 2017 | 28424670 |
| efficacy and safety of probiotic-supplemented triple therapy for eradication of helicobacter pylori in children: a systematic review and network meta-analysis. | the aim of this study was to identify the best probiotic supplementation in triple therapy for pediatric population with helicobacter pylori infection. | 2017 | 28681177 |
| [bifidobacterium scardovii isolated from 2 consecutive urine samples]. | bifidobacterium scardovii species consists of facultative anaerobic gram-positive rods whose growth is stimulated by co2 and anaerobiosis. exceptionally it has been associated with infections in humans. an elderly male patient with a urinary tract infection due to b. scardovii and enterococcus faecalis is presented here; both microorganisms were isolated from two consecutive urine samples. the bacillus did not grow on standard media, but on chocolate agar incubated in co2 and on supplemented bru ... | 2017 | 28734711 |
| in vitro evaluation of cytotoxic, anti-proliferative, anti-oxidant, apoptotic, and anti-microbial activities of cladonia pocillum. | the aim of this study was to investigate the anti-proliferative, apoptotic, cytotoxic, and anti-oxidant effects of extracts from the lichen cladonia pocillumon human breast cancer cells (mcf-7), and to characterize the anti-microbial features. mcf-7 cells were treated with methanolic c. pocillum extract for 24h. the cytotoxicity of the extract was tested with mtt. moreover, its anti-proliferative effects were examined with immunocytochemical method. apoptosis and biochemical parameters were det ... | 2017 | 28838343 |
| investigation of effect of 1,8-cineole on antimicrobial activity of chlorhexidine gluconate. | chlorhexidine belongs to a group of medicines called antiseptic antibacterial agents. chlorhexidine is commonly used for the care and clean off the skin, hands, and wounds. in recent years, medicinal and aromatic plants have been used for prevention of disease, maintaining health, and improving disease in traditional and modern medicine as a medicament. according to recent research, cineole is the isolated active agent of eucalyptus oil and possesses antimicrobial activity. it was demonstrated t ... | 2017 | 28827963 |
| proinflammatory mg-63 cells response infection with enterococcus faecalis cps2 evaluated by the expression of tlr-2, il-1β, and inos mrna. | we have previously demonstrated that unencapsulated enterococcus faecalis cps2 inhibits biofilm formation of candida albicans, a fungus commonly found with e. faecalis in periapical lesion. in this study, we compared encapsulated and unencapsulated e. faecalis cps2 strains relationship with osteoblastic (mg-63) cells, whereas e. faecalis atcc 29212 were used as a reference strain. | 2017 | 28800779 |
| antibacterial and antifungal activity of endodontic intracanal medications. | the sterilization of the entire root canal system represents the main goal of every endodontist, given the fact that the control of the microbial flora is the key point of every root canal treatment. the diversity of microorganisms found inside the root canal and also the resistance of some bacterial species to intracanal medications led to a continuous development of new endodontic products. the present study focuses on the comparison of the antibacterial and antifungal properties of different ... | 2017 | 28781531 |
| antimicrobial and immunomodulatory activity of host defense peptides, clavanins and ll-37, in vitro: an endodontic perspective. | endodontic treatment is mainly based on root canal disinfection and its failure may be motivated by microbial resistance. endodontic therapy can be benefitted by host defense peptides (hdps), which are multifunctional molecules that act against persistent infection and inflammation. this study aimed to evaluate the antimicrobial, cytotoxic and immunomodulatory activity of several hdps, namely clavanin a, clavanin a modified (mo) and ll-37, compared to intracanal medication ca(oh)2. hdps and ca(o ... | 2017 | 28712894 |
| thymus vulgaris l. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity. | this study evaluated the biological effects of the t. vulgaris l. extract., such as antimicrobial activity on planktonic cultures and mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory activity and genotoxicity. | 2017 | 28683409 |
| comparison of the antimicrobial efficacy of calcium hydroxide and photodynamic therapy against enterococcus faecalis and candida albicans in teeth with periapical lesions; an in vivo study. | introduction: elimination of pathological microflora of root canal systems is a major goal in endodontic treatment. this study aimed to compare the antimicrobial efficacy of calcium hydroxide as an intracanal medication and antibacterial photodynamic therapy (apdt) against enterococcus faecalis and candida albicans in teeth with periapical (pa) lesions. methods: this in vivo study was conducted on 20 patients with single-rooted mandibular premolar with previously failed endodontic treatment. thi ... | 2017 | 28652899 |
| in vitro evaluation of antimicrobial activity of alimentary canal extracts from the red palm weevil, rhynchophorus ferrugineus olivier larvae. | the invasive red palm weevil, rhynchophorus ferrugineus olivier (coleoptera: curculionidae), is considered one of the world's most devastating insect pests to palm trees. it was observed that larvae of this pest are able to inhibit microbial growth on the rearing media when they start feeding and this observation has led us to study the effect of red palm weevils on various microbial species. the antimicrobial effect of extracts from different parts of the alimentary canal on gram positive bacte ... | 2017 | 28612029 |
| formulation and evaluation of sol-gel drug delivery system for intracanal ph sensitive controlled delivery of chlorhexidine. | persistence of viable micro-organisms even after thorough chemomechanical debridement has been cited as a major cause for endodontic failure. chlorhexidine is a drug, which has shown marked efficacy against enterococcus faecalis and candida spp., which are mostly accounted for endodontic failure and it has demonstrated high degree of substantivity to dentin by adsorption. another issue with chlorhexidine and other intracanal medicaments is the excessive or premature peaking of the drug leading t ... | 2017 | 28571266 |
| antimicrobial and anti-biofilm properties of polypropylene meshes coated with metal-containing dlc thin films. | a promising strategy to reduce nosocomial infections related to prosthetic meshes is the prevention of microbial colonization. to this aim, prosthetic meshes coated with antimicrobial thin films are proposed. commercial polypropylene meshes were coated with metal-containing diamond-like carbon (me-dlc) thin films by the magnetron sputtering technique. several dissimilar metals (silver, cobalt, indium, tungsten, tin, aluminum, chromium, zinc, manganese, tantalum, and titanium) were tested and com ... | 2017 | 28560581 |
| application of hyperosmotic nanoemulsions in wound healing: partial thickness injury model in swine. | objective: in this work, we introduce a novel hyperosmotic nanoemulsion (hne) topical agent for use in wound healing. these topical emulsion complexes combine a lipophilic thymol nanoemulsion with a hyperosmotic saccharide matrix. this combination has been previously shown to possess synergistic antimicrobial activity against a host of common and drug-resistant pathogens in vitro. approach: in this study, we present additional data to assess the safety and efficacy of these emulsions in a partia ... | 2017 | 28507786 |
| microbiological analysis of acute infections of the nail fold on the basis of bait thread test. | an acute infection of the nail fold, called paronychia, is a common clinical problem. the basis for the implementation of the treatment is the result of microbiological examination. due to the rapid and painful course of infection, usually an empirical antimicrobial treatment prior to obtaining microbiological test results is introduced. | 2017 | 28507488 |
| comparative evaluation of antimicrobial efficacy of triple antibiotic paste, calcium hydroxide, and a proton pump inhibitor against resistant root canal pathogens. | the objective of this study is to compare the antimicrobial efficacy of triple antibiotic paste (tap) and a proton pump inhibitor (ppi) (omeprazole) in combination with calcium hydroxide (ch) against enterococcus faecalis and candida albicans. | 2017 | 28435366 |
| efficacy of three different lasers on eradication of enterococcus faecalis and candida albicans biofilms in root canal system. | the objective was to compare the efficacy of three different lasers in disinfection of root canals inoculated with enterococcus faecalis and candida albicans biofilms. | 2017 | 28426292 |
| antimicrobial photodynamic therapy against endodontic enterococcus faecalis and candida albicans mono and mixed biofilms in the presence of photosensitizers: a comparative study with classical endodontic irrigants. | endodontic biofilms eradication from the infected root canal system remains as the primary focus in endodontic field. in this study, it was assessed the efficacy of antimicrobial photodynamic therapy (apdt) with the zn(ii)chlorin e6 methyl ester (zn(ii)e6me) activated by red light against monospecies and mixed biofilms of enterococcus faecalis and candida albicans. the results were compared with the ones obtained with rose bengal (rb), toluidine blue-o (tbo), the synthetic tetracationic porphyri ... | 2017 | 28424663 |
| antimicrobial and antibiofilm activities of mta supplemented with bismuth lipophilic nanoparticles. | the objective of this work was to determine the antimicrobial and antibiofilm properties of mineral trioxide aggregate (mta) supplemented with bismuth lipophilic nanoparticles (bisbal nps). the antimicrobial activity of the composite mta-bisbal nps was determined by the disk diffusion assay, while antibiofilm activity was analyzed by fluorescence microscopy. the cytotoxicity of mta-bisbal nps was determined on human gingival fibroblasts by optical microscopy and crystal violet staining. mta-bisb ... | 2017 | 28420830 |
| nitric oxide charged catheters as a potential strategy for prevention of hospital acquired infections. | catheter-associated hospital-acquired infections (hai's) are caused by biofilm-forming bacteria. using a novel approach, we generated anti-infective barrier on catheters by charging them with nitric oxide (no), a naturally-produced gas molecule. no is slowly released from the catheter upon contact with physiological fluids, and prevents bacterial colonization and biofilm formation onto catheter surfaces. | 2017 | 28410367 |
| enterococcus faecalis bacteriocin entv inhibits hyphal morphogenesis, biofilm formation, and virulence of candida albicans. | enterococcus faecalis, a gram-positive bacterium, and candida albicans, a fungus, occupy overlapping niches as ubiquitous constituents of the gastrointestinal and oral microbiome. both species also are among the most important and problematic, opportunistic nosocomial pathogens. surprisingly, these two species antagonize each other's virulence in both nematode infection and in vitro biofilm models. we report here the identification of the e. faecalis bacteriocin, entv, produced from the entv (ef ... | 2017 | 28396417 |
| honeybee (apis mellifera)-associated bacterial community affected by american foulbrood: detection of paenibacillus larvae via microbiome analysis. | honeybee (apis mellifera l.) workers act as passive vectors of paenibacillus larvae spores, which cause the quarantine disease american foulbrood (afb). we assessed the relative proportions of p. larvae within the honeybee microbiome using metabarcoding analysis of the 16 s rrna gene. the microbiome was analyzed in workers outside of the afb zone (control - afb0), in workers from asymptomatic colonies in an afb apiary (afb1), and in workers from colonies exhibiting clinical afb symptoms (afb2). ... | 2017 | 28698604 |
| vancomycin-resistant enterococci with vana gene in treated municipal wastewater and their association with human hospital strains. | vancomycin-resistant enterococci (vre) are pathogens of increasing medical importance. in brno, czech republic, we collected 37 samples from the effluent of a wastewater treatment plant (wwtp), 21 surface swabs from hospital settings, and 59 fecal samples from hospitalized patients and staff. moreover, we collected 284 gull cloacal swabs from the colony situated 35km downstream the wwtp. samples were cultured selectively. enterococci were identified using maldi-tof ms, phenotypically tested for ... | 2017 | 28763660 |
| the potential of alcohol release doorplates to reduce surface contamination during hand contact. | optimal hand hygiene may be compromised by contact with contaminated environmental surfaces. | 2017 | 28760635 |
| inhibition of clostridium difficile in mice using a mixture of potential probiotic strains enterococcus faecalis nm815, e. faecalis nm915, and e. faecium nm1015: novel candidates to control c. difficile infection (cdi). | this study is aimed at the isolation, identification, and characterization of potential probiotic strains capable of inhibiting clostridium difficile in vitro and in vivo. twenty isolates were isolated from infant fecal samples and screened against c. difficile using their cell-free supernatant. only three isolates showed maximum inhibition from 56.05 to 60.60%, thus they were characterized for probiotic properties and safety. the results obtained approved their tolerance to the gastrointestinal ... | 2017 | 28497217 |
| eco-friendly fabrication of ag nanostructures using the seed extract of pedalium murex, an ancient indian medicinal plant: histopathological effects on the zika virus vector aedes aegypti and inhibition of biofilm-forming pathogenic bacteria. | the control of zika virus mosquito vectors and well as the development of drugs in the fight against biofilm-forming microbial pathogens, are timely and important challenges in current bionanoscience. here we focused on the eco-friendly fabrication of ag nanostructures using the seed extract of pedalium murex, an ancient indian medicinal plant. initial confirmation of ag nanoparticles (agnps) production was showed by a color change from transparent to dark brown. the uv-visible spectrum (476nm), ... | 2017 | 28772238 |
| microbial analysis of root canal and periradicular lesion associated to teeth with endodontic failure. | the quantification of ten microorganisms at the root ends and in the surrounding periradicular lesions was performed. thirty 3 mm samples root ends and 30 samples of the surrounding chronic periapical infection were collected during apical microsurgery. samples were triturated, and the bacterial dna was obtained. the bacterial quantification was performed by using the sybr green system. at least one microorganism was detected in all patients. in both the root end and periapical samples, fusobact ... | 2017 | 28666877 |
| antibiotic activity of emerimicin iv isolated from emericellopsis minima from talcahuano bay, chile. | due to the increasing emergence of resistance of bacterial pathogens to current antibiotics, we have examined the marine fungi present in sea sediments obtained 200 m offshore to discover new antibacterial compounds active against multidrug-resistant bacteria. one strain, identified as emericellopsis minima, was isolated from sediments of talcahuano bay (chile). from the liquid culture of e. minima, we isolated emerimicin iv, a unique fungal peptaibol that exhibited antibacterial activity. the s ... | 2017 | 28669240 |
| conjugal transfer of aac(6')ie-aph(2″)ia gene from native species and mechanism of regulation and cross resistance in enterococcus faecalis mcc3063 by real time-pcr. | high level aminoglycoside resistance (hlar) in the lactic acid bacteria (lab) derived from food animals is detrimental. the aim of this study was to investigate the localization and conjugal transfer of aminoglycoside resistance genes, aac(6')ie-aph(2″)ia and aph(3')iiia in different enterococcus species. the cross resistance patterns in enterococcus faecalis mcc3063 to clinically important aminoglycosides by real time pcr were also studied. southern hybridization experiments revealed the presen ... | 2017 | 28774859 |
| bloodstream infections caused by enterococcus spp: a 10-year retrospective analysis at a tertiary hospital in china. | in order to discover the risk factors for 30-day mortality in bloodstream infections (bsi) caused by enterococcus spp. strains, we explored the clinical and therapeutic profile of patients with enterococcus spp. bsi and the characteristics of this condition. a total of 64 patients with bsi caused by enterococcus spp. who were treated in our hospital between 2006 and 2015 were included in the study. the clinical features of patients, microbiology, and 30-day mortality were collected from the elec ... | 2017 | 28397040 |
| the individual contribution of starter and non-starter lactic acid bacteria to the volatile organic compound composition of caciocavallo palermitano cheese. | the contribution of two starter (lactobacillus delbrueckii and streptococcus thermophilus) and nine non-starter (enterococcus casselliflavus, enterococcus faecalis, enterococcus durans, enterococcus gallinarum, lactobacillus casei, lactobacillus paracasei, lactobacillus rhamnosus, pediococcus acidilactici and pediococcus pentosaceus) species of lactic acid bacteria (lab) to the volatile organic compounds (vocs) of caciocavallo palermitano cheese was investigated. the strains used in this study w ... | 2017 | 28783535 |
| diversity of antagonistic bacteria isolated from medicinal plant peganum harmala l. | the antimicrobial activity of plant extract of peganum harmala, a medicinal plant has been studied already. however, knowledge about bacterial diversity associated with different parts of host plant antagonistic to different human pathogenic bacteria is limited. in this study, bacteria were isolated from root, leaf and fruit of plant. among 188 bacterial isolates isolated from different parts of the plant only 24 were found to be active against different pathogenic bacteria i.e. escherichia coli ... | 2017 | 28855824 |
| effect of conventional irrigation and photoactivated disinfection on enterococcus faecalis in root canals: an in vitro study. | a study was done to evaluate the antimicrobial efficacy of sodium hypochlorite (naocl) and photoactivated disinfection (pad) on enterococcus faecalis. | 2017 | 28855761 |
| enhancing antibacterial effect of sodium hypochlorite by low electric current-assisted sonic agitation. | this research focused on the effects of low electric current (μe)-assisted sonic agitation of sodium hypochlorite on enterococcus faecalis infected human root dentin. | 2017 | 28854274 |
| discovery of phylloseptins that defense against gram-positive bacteria and inhibit the proliferation of the non-small cell lung cancer cell line, from the skin secretions of phyllomedusa frogs. | the growing occurrence of bacterial resistance to conventional antibiotics has called for the development of new classes of antimicrobial agents. antimicrobial peptides (amps) with broad antimicrobial spectrum derived from frog skin secretions have been demonstrated to be promising candidates for new antibiotic development. a proven rich source of these compounds are the skin secretions of the frogs in the phyllomedusa genus. in this study, two novel phylloseptin peptides-phylloseptin-pta and ph ... | 2017 | 28850103 |
| infectious endocarditis from enterococcus faecalis associated with tubular adenoma of the sigmoid colon. | enterococcus faecalis (e. faecalis), a constituent of the gut microbiota, can be associated with both colonic lesions and endocarditis. since this microorganism is one of the endocarditis etiological agents, there is a need for greater study in regard to the association with endocarditis and colonic lesions. | 2017 | 28848681 |
| molecular determinants of the matrix thickening of a dual-species pseudomonas aeruginosa and enterococcus faecalis biofilm. | biofilms are microbial communities that inhabit various surfaces and are surrounded by extracellular matrices (ecms). clinical microbiologists have shown that the majority of chronic infections are caused by biofilms, after introduction of the first biofilm infection model by j.w. costerton and his colleagues (1). however, treatments for chronic biofilm infections are still limited to surgical removal of the infected sites. pseudomonas aeruginosa and enterococcus faecalis are two frequently iden ... | 2017 | 28842537 |
| how proteases from enterococcus faecalis contribute to its resistance to short α-helical antimicrobial peptides. | hyl-20 (gilsslwkklkkiiak-nh2) is an analogue of a natural antimicrobial peptide (amp) previously isolated from the venom of wild bee. we examined its antimicrobial activity against three strains of enterococcus faecalis while focusing on its susceptibility to proteolytic degradation by two known proteases-gelatinase (gele) and serine protease (spre)-which are secreted by these bacterial strains. we found that hyl-20 was primarily deamidated at its c-terminal which made the peptide susceptible to ... | 2017 | 28830077 |
| small alarmone synthetases as novel bacterial rna-binding proteins. | the alarmone nucleotides guanosine pentaphosphate (pppgpp) and tetraphosphate (ppgpp), collectively referred to as (p)ppgpp, are key regulators of bacterial growth, stress adaptation, antibiotic tolerance and pathogenicity. we have recently shown that the small alarmone synthetase (sas) relq from the gram-positive pathogen enterococcus faecalis has an rna-binding activity (beljantseva et al. 2017). relq's activities as an enzyme and as a rna-binding protein are mutually incompatible: binding of ... | 2017 | 28820325 |
| [elimination of entercoccus faecalis with different disinfection methods in root canals in vitro]. | to compare the elimination effect against e.faecalis in root canals with different methods. | 2017 | 28815239 |
| antibacterial properties of nonwoven wound dressings coated with manuka honey or methylglyoxal. | manuka honey (mh) is used as an antibacterial agent in bioactive wound dressings via direct impregnation onto a suitable substrate. mh provides unique antibacterial activity when compared with conventional honeys, owing partly to one of its constituents, methylglyoxal (mgo). aiming to investigate an antibiotic-free antimicrobial strategy, we studied the antibacterial activity of both mh and mgo (at equivalent mgo concentrations) when applied as a physical coating to a nonwoven fabric wound dress ... | 2017 | 28813014 |
| in vitro antiplasmodial activity and prophylactic potentials of extract and fractions of trema orientalis (linn.) stem bark. | trema orientalis (t. orientalis linn) has been used in the management of malaria in the western part of nigeria and despite its application in ethnomedicine, there is dearth of scientific evidence to justify the acclaimed prophylactic antimalarial usage of the plant. the aim of this study is to assess the in vitro antiplasmodial cell-free assay and chemopreventive efficacy of the methanol extract of the stem bark of t. orientalis and its fractions as a prophylactic regimen for malaria prevention ... | 2017 | 28810849 |
| growth- and stress-induced pasta kinase phosphorylation in enterococcus faecalis. | transmembrane ser/thr kinases containing extracellular pasta domains are ubiquitous among actinobacteria and firmicutes. such pasta kinases regulate critical processes including antibiotic resistance, cell division, toxin production, virulence, and are essential for viability in certain organisms. based on in vitro studies with purified extra- and intracellular fragments of pasta kinases, a model for signaling has been proposed, in which the extracellular pasta domains bind as-yet-undefined liga ... | 2017 | 28808126 |
| genomic insights into the pathogenicity and environmental adaptability of enterococcus hirae r17 isolated from pork offered for retail sale. | genetic information about enterococcus hirae is limited, a feature that has compromised our understanding of these clinically challenging bacteria. in this study, comparative analysis was performed of e. hirae r17, a daptomycin-resistant strain isolated from pork purchased from a retail market in beijing, china, and three other enterococcal genomes (enterococcus faecium do, enterococcus faecalis v583, and e. hirae atcc(™) 9790). some 1,412 genes were identified that represented the core genome t ... | 2017 | 28799224 |
| methyl propiolate and 3-butynone: starting points for synthesis of amphiphilic 1,2,3-triazole peptidomimetics for antimicrobial evaluation. | a library of 29 small 1,4-substituted 1,2,3-triazoles was prepared for studies of antimicrobial activity. the pharmacophore model investigated with these substrates was based on small peptidomimetics of antimicrobial peptides and antimicrobials isolated from marine organisms from sub-arctic regions. using methyl 1,2,3-triazole-carboxylates and 1,2,3-triazole methyl ketones prepared through "click" chemistry we were able to synthesize the different cationic amphiphiles through three steps or less ... | 2017 | 28797773 |
| media from macrophages co-incubated with enterococcus faecalis induces epithelial cell monolayer reassembly and altered cell morphology. | signal exchange between intestinal epithelial cells, microbes and local immune cells is an important mechanism of intestinal homeostasis. given that intestinal macrophages are in close proximity to both the intestinal epithelium and the microbiota, their pathologic interactions may result in epithelial damage. the present study demonstrates that co-incubation of murine macrophages with e. faecalis strains producing gelatinase (gele) and serine protease (spre) leads to resultant condition media ( ... | 2017 | 28793333 |
| anti-enterococcal activities of pentacyclic triterpenes. | asiatic (aa) and ursolic (ua) acids are widely studied phytochemicals, but their antimicrobial properties are still poorly understood. therefore our research has focused on their activity against uropathogenic enterococcus faecalis strains. | 2017 | 28791824 |
| characterization of the tertiary structure of the peptidoglycan of enterococcus faecalis. | solid-state nmr spectra of whole cells and isolated cell walls of enterococcus faecalis grown in media containing combinations of (13)c and (15)n specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan. the compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross- ... | 2017 | 28784459 |
| coupling metagenomics with cultivation to select host-specific probiotic microorganisms for subtropical aquaculture. | to demonstrate a non-empirical workflow to select host-specific probiotics for aquaculture industry. | 2017 | 28783248 |
| septic episodes in a premature infant after in utero exposure to rituximab. | rituximab is an increasingly used immunotherapeutic agent for women of reproductive age for treatment of autoimmune diseases, leukemias, and lymphomas. rituximab is a chimeric monoclonal antibody that targets b-cell surface antigen cd20 and can cross the placenta. current evidence of the impact of this medication on the developing fetus is limited, but there is little to suggest that fetal exposure to this medication places an infant at increased risk of immunosuppression and subsequent infectio ... | 2017 | 28768852 |
| microbial load in septic and aseptic procedure rooms. | highly effective measures to prevent surgical wound infections have been established over the last two decades. we studied whether the strict separation of septic and aseptic procedure rooms is still necessary. | 2017 | 28764834 |
| fabrication and appraisal of poly (lactic-co-glycolic acid) - moxifloxacin nanoparticles using vitamin e-tpgs: a potential intracanal drug delivery agent. | nanotechnology is gaining momentum in the search for ideal biomaterials by dental researchers. the expansible nature of endodontology deems exploration, learning and scrutinizing newer avenues which have the potential to be applied and investigated. the popularity of polymers as drug delivery vehicles has opened avenues for their application in the root canal system. this study utilized application of biodegradable polymers as potential drug delivery vehicles against enterococcus faecalis, one o ... | 2017 | 28764283 |
| revisiting nitrofurantoin for vancomycin resistant enterococci. | enterococcal infection has emerged as a major therapeutic challenge. emergence of high level aminoglycoside resistance (hlar) and vancomycin-resistant enterococcus (vre) has further limited the drug therapy in enterococcal infections. however, nitrofuratoin being an old drug reported to have less resistance in comparison to the other classes of antimicrobial agents. | 2017 | 28764160 |
| additive effect of photoactivated disinfection on the antibacterial activity of qmix 2in1 against 6-week enterococcus faecalis biofilms: an in vitro study. | evaluation of the additive effect of photodynamic therapy (pdt) on the antibacterial activity of 2.5% sodium hypochlorite (naocl) and qmix against 6-week enterococcus faecalis biofilms contaminated root canals. | 2017 | 28761252 |
| first case of multidrug-resistant enterococcus faecalis isolated from herding horse in xinjiang, western china. | 2017 | 28760414 | |
| in vitro synergistic activities of cefazolin and nisin a against mastitis pathogens. | first-generation cephalosporins such as cefazolin (cez) have been widely used for mastitis treatment in dairy cattle. however, the use of antibiotics results in the presence of antibiotic residues in milk, which is used for human consumption. nisin a, a bacteriocin produced by lactococcus lactis, has been used as a broad-spectrum food preservative for over 50 years. therefore, a combination of cez and nisin a might provide an extended activity spectrum against mastitis pathogens and reduce the a ... | 2017 | 28757508 |
| efficacy of photon-induced photoacoustic streaming in the reduction of enterococcus faecalis within the root canal: different settings and different sodium hypochlorite concentrations. | the purpose of this study was to determine the effectiveness of laser-activated irrigation by photon-induced photoacoustic streaming (pips) in the reduction of enterococcus faecalis in root canal disinfection, varying laser energy output, and sodium hypochlorite (naocl) concentration. for effective removal of the smear layer, the sequence and resting time of the final irrigation steps were modified compared with the standard pips protocol. | 2017 | 28756961 |
| draft genome sequence of enterococcus faecalis dd14, a bacteriocinogenic lactic acid bacterium with anti-clostridium activity. | we report the draft genome sequence of enterococcus faecalis dd14, a strain isolated from meconium of a healthy newborn at roubaix hospital (france). the strain displayed antagonism against a set of gram-positive bacteria through concomitant production of lactic acid and bacteriocin. the genome has a size of 2,893,365 bp and a 37.3% g+c ratio and is predicted to contain at least 2,755 coding sequences and 62 rnas. | 2017 | 28751398 |
| photodynamic therapy with high-power led mediated by erythrosine eliminates enterococcus faecalis in planktonic forms. | the failure of endodontic treatment is linked to the presence of microorganisms, particularly enterococcus faecalis, in the root canals. this study evaluated the effectiveness of photodynamic therapy (pdt) using erythrosine irradiated by a high-power curing light on a planktonic suspension culture of e. faecalis. | 2017 | 28750753 |
| buwchitin: a ruminal peptide with antimicrobial potential against enterococcus faecalis. | antimicrobial peptides (amps) are gaining popularity as alternatives for treatment of bacterial infections and recent advances in omics technologies provide new platforms for amp discovery. we sought to determine the antibacterial activity of a novel antimicrobial peptide, buwchitin, against enterococcus faecalis. buwchitin was identified from a rumen bacterial metagenome library, cloned, expressed and purified. the antimicrobial activity of the recombinant peptide was assessed using a broth mic ... | 2017 | 28748180 |
| assessment of facultative anaerobes from the root canals of deciduous molars: an in vivo study. | background. the current research aimed to assess the prevalence of facultative anaerobes isolated from the root canals of deciduous molars. methods. the present research enrolled 60 children in the 6‒9-year age group based on clinical and radiographic findings. under aseptic conditions, access cavities were prepared followed by collection of samples from infected root canals with the help of sterile paper points. the samples thus obtained were subjected for microbial assay. results. it was found ... | 2017 | 28748050 |
| effect of different mixing methods on the bacterial microleakage of white portland cement and white mineral trioxide aggregate. | background. of this study was to investigate the effect of different mixing methods (ultrasonic, amalgamator, and conventional) on the bacterial microleakage of white portland cement (wpc) and white mta (tooth-colored formula, dentsply, tulsa dental, tulsa, ok). methods. a hundred human single-rooted permanent teeth were decoronated to obtain 14 mm of root length in all the samples. the root canals were cleaned, shaped and obturated. three millimeters of each root apex were cut off and randomly ... | 2017 | 28748048 |
| determination of disk diffusion and mic quality control ranges for nafithromycin (wck 4873), a new lactone-ketolide. | disk diffusion and mic quality control (qc) ranges were determined for nafithromycin, a new lactone-ketolide, following completion of a nine-laboratory, clinical and laboratory standards institute (clsi) m23-defined tier 2 study. five qc strains consistent with the spectrum of activity of nafithromycin were tested: staphylococcus aureus atcc 25923 (disk only), s. aureus atcc 29213 (broth only), enterococcus faecalis atcc 29212 (broth only), streptococcus pneumoniae atcc 49619 (disk and broth), a ... | 2017 | 28747372 |
| bacterial size matters: multiple mechanisms controlling septum cleavage and diplococcus formation are critical for the virulence of the opportunistic pathogen enterococcus faecalis. | enterococcus faecalis is an opportunistic pathogen frequently isolated in clinical settings. this organism is intrinsically resistant to several clinically relevant antibiotics and can transfer resistance to other pathogens. although e. faecalis has emerged as a major nosocomial pathogen, the mechanisms underlying the virulence of this organism remain elusive. we studied the regulation of daughter cell separation during growth and explored the impact of this process on pathogenesis. we demonstra ... | 2017 | 28742152 |
| characterization of enterococcus faecalis isolates by chicken embryo lethality assay and eric-pcr. | enterococcus faecalis is the major causative agent of amyloid arthropathy in chickens. given the difficulty of estimating the risk from field strains, the embryo lethality assay (ela) is proposed in this study as a model to predict the virulence of 68 avian e. faecalis strains. additionally, enterobacterial repetitive intergenic consensus polymerase chain reaction (eric-pcr) was used to characterize the genetic diversity of the e. faecalis strains. the ela was performed 10 times with subsets of ... | 2017 | 28737107 |
| genetic diversity and antimicrobial resistance profile of enterococcus faecalis isolated from broilers with vertebral osteomyelitis in southeast brazil. | vertebral osteomyelitis (vo) is a worldwide emerging disease that affects broilers. recently, the isolation of enterococcus faecalis in cases of the disease has been described. this study aimed at determining the genetic diversity and antimicrobial resistance profile of 12 enterococcus faecalis strains isolated from broilers with vo. strains were isolated from nine flocks from six farms in a high-density poultry production area in southeast brazil and were evaluated using multilocus sequence typ ... | 2017 | 28737063 |
| the usefulness of biosynthetic vascular graft omniflow ii and autologous veins for the treatment of massive infection of dacron vascular graft with enterococcus faecalis hlar. | infections of vascular grafts are the most severe complications in vascular surgery. we present the case of a 73-year-old male with infection of a dacron prosthesis with a strain of enterococcus faecalis. the patient was treated with replacement of a full prosthesis with a combined graft constructed from biosynthetic vascular graft omniflow and autologous veins. this graft is recommended for implantation in patients with a higher risk of infection. our case is one of the first reported usage of ... | 2017 | 28735332 |
| activity of dalbavancin tested against gram-positive clinical isolates causing skin and skin structure infections in paediatric patients from us hospitals (2014 - 2015). | this study provides an in vitro analysis of dalbavancin activity against isolates causing skin and skin structure infections (sssis) in children. | 2017 | 28735053 |