Publications

identification of thermophilic bacteria in solid-waste composting.	the thermophilic microbiota of solid-waste composting, with major emphasis on bacillus spp., was examined with trypticase soy broth (bbl microbiology systems) with 2% agar as the initial plating medium. five 4.5-liter laboratory units at 49 to 69 degrees c were fed a mixture of dried table scraps and shredded newspaper. the composting plants treating refuse at altoona, pa., and refuse-sludge at leicester, england, were also sampled. of 652 randomly picked colonies, 87% were identified as bacillu ...	1985	4083886
cultivation of fungi in synthetic and semi-synthetic liquid medium. i. growth characteristics of the fungi and biochemical properties of the isolated antigenic material.	four allergologically important fungi, viz. aspergillus fumigatus, alternaria penicillium notatum, and cladosporium herbarum, were cultured in a pure synthetic medium and the patterns of growth as characterized by the ph, protein and carbohydrate concentration of the culture fluid, were studied. a fumigatus and p. notatum showed a similar growth pattern, characterized by a rapid decrease in the ph of the culture medium (ph 7.4----4.0), while proteins were slowly released and saccharose poorly co ...	1985	4091234
detection of antigens in lung biopsies by immunoperoxidase staining in extrinsic allergic bronchiolo-alveolitis (eaba).	open lung biopsy specimens of 14 patients with extrinsic allergic bronchiolo-alveolitis (eaba) were studied by immunoperoxidase staining with avidin-biotin-complex method using nonconjugated antisera to micropolyspora faeni, thermoactinomyces vulgaris, aspergillus fumigatus, and aspergillus umbrosus. antigenic intracellular material was found in macrophages and giant cells of granulomas in the lung tissue of 8 patients. this finding supports the hypothesis which suggests that macrophages and t-l ...	1985	2409726
identification of an agent in cultures of aspergillus fumigatus displaying anti-phagocytic and immunomodulating activity in vitro.	when cultured in vitro, aspergillus fumigatus generated a metabolite(s) with anti-phagocytic activity as tested by macrophage adherence to plastic and phagocytosis of particulate matter. the metabolite(s) appeared after 3 d culture and reached a peak concentration after 5-6 d. the action of the anti-phagocytic agent(s) was rapid (5-15 min) and appeared not to alter membrane permeability or cause rapid cell death. treatment of stimulator spleen cells with the agent(s) inhibited their ability to i ...	1985	2410548
a rapid colorimetric assay of fungal viability with the tetrazolium salt mtt.	a rapid colorimetric assay for viability of fungi that uses the tetrazolium salt mtt is described. all live species of a wide range of medically important fungi that were tested reduced mtt, with resultant intracellular formation within several hours of clearly visible purple crystals of mtt-formazan. the assay proved particularly useful in measuring viability of individual hyphae; greater than 99% of live aspergillus fumigatus and rhizopus oryzae hyphae stained purple when incubated with mtt co ...	1985	2413145
bronchial allergy in cystic fibrosis.	thirty-one patients with cystic fibrosis (cf) were thoroughly evaluated for allergy. this included a clinical history, skin tests with twenty-three allergens and bronchial provocation with inhaled allergens and histamine. the bronchial response was measured by whole body plethysmography. of the patients studied, 40% showed a bronchoconstrictor response to inhaled allergens, despite the fact that none had reported asthma in their clinical history. strong skin test reactions (3+ and 4+) and weak r ...	1985	2414033
standardization of allergenic extracts of aspergillus fumigatus. liberation of ige-binding components during cultivation.	during cultivation of aspergillus fumigatus a rapid liberation of ige-binding components was found reaching maximum values during the logarithmic phase of growth (phase i). after a fall in ige-binding titers during phase ii, appearance of additional ige-binding components was noted during the period of lysis of the microorganism (phase iii). these latter allergenic components are different from the phase i ige-binding components, as was shown by crossed-inhibition studies. the number of precipit ...	1985	2578438
[invasive aspergillar pneumopathy during the treatment of microcellular bronchial cancer. efficacy of early antifungal treatment].	the invasive aspergillus pneumonias have been described particularly in chemotherapy for patients with haematological disorders. in respiratory disorders such cases are exceptional. the authors report a case of invasive aspergillus pneumonia, occurring during treatment of a small cell cancer; the rapid commencement of anti-fungal treatment by amphotericin "b" and flucytosine enabled an apparent cure of the tumour by radiotherapy and chemotherapy. the authors stress the difficulty of definitive d ...	1985	3012720
[clinical aspects of pulmonary aspergilloma (31 cases)].		1985	3012754
neutrophil chemotactic responses induced by fresh and swollen rhizopus oryzae spores and aspergillus fumigatus conidia.	with the induction of germination, rhizopus oryzae spores and aspergillus fumigatus conidia activate the complement system and induce neutrophil chemotaxis. in contrast, freshly isolated r. oryzae spores did not induce neutrophil migration into lung tissue of mice after intranasal inoculation. moreover, in microchemotaxis assays neither fresh r. oryzae spores nor a. fumigatus conidia activated sera to stimulate human neutrophil chemotaxis above control migration until at least 10(7) or 10(8) spo ...	1985	3157647
neurological manifestations of the acquired immunodeficiency syndrome (aids): experience at ucsf and review of the literature.	in this review of the acquired immunodeficiency syndrome (aids), the authors have evaluated a total of 352 homosexual patients with aids or generalized lymphadenopathy managed at the university of california, san francisco (ucsf), between 1979 and 1984. of an initial unselected group of 318 patients, 124 (39%) were neurologically symptomatic, and one-third already had their neurological complaints at the time of presentation. an additional 210 aids patients with neurological symptoms have been r ...	1985	2983051
the action of itraconazole and ketoconazole on growth and sterol synthesis in aspergillus fumigatus and aspergillus niger.	growth and sterol synthesis of aspergillus fumigatus and a. niger were studied in control cultures and in the presence of ketoconazole or itraconazole, the latter compound being 100 times more growth inhibitory than the former. sterol synthesis is inhibited more rapidly than any visible fungal outgrowth. this inhibition results in an accumulation of 4,14 dimethyl- and 4,4',14 trimethylsterols. the presence of these membrane-disturbing sterols may result in a pertubation of membrane-bound enzyme ...	1985	2986303
mechanisms of resistance of aspergillus fumigatus conidia to killing by neutrophils in vitro.	despite the critical role for neutrophils in host defenses against invasive aspergillosis, previous studies have established that neutrophils are unable to kill resting conidia of aspergillus fumigatus. the mechanisms of resistance of the conidia were therefore investigated. electron microscopy studies showed the fusion of phagosomes containing a. fumigatus conidia with lysosomes of the neutrophil. resting conidia of a. fumigatus were then compared with those that had been preincubated in broth ...	1985	2989387
[experimental renal infection with aspergillus fumigatus in mice: a chemotherapeutic model of an organic mycosis].		1985	2989682
itraconazole treatment of murine aspergillosis.	icr mice were challenged with conidia of aspergillus fumigatus given either intranasally or intravenously, and treated beginning 1 day later with itraconazole, amphotericin b, or the vehicles for these two agents. mice challenged intravenously and treated with either antifungal drug had prolonged survival over controls, and had lower tissue counts in the kidneys than the controls. however, mice challenged intranasally had neither prolonged survival nor lower lung tissue counts than the controls.	1985	2992106
gas holdup in an external-loop air-lift fermentor with a contaminated gas distributor.	contamination of the porous-plate gas distributor was observed in an air-lift fermentor used for aspergillus fumigatus fermentations. comparisons between gas holdups measured with uncontaminated distributors and the contaminated one showed that the contamination had no effect on gas holdups in water but reduced the holdups in sodium chloride and sodium sulfate solutions, probably due to increased bubble coalescence. a correlation for gas holdups with clean distributors(2) could be used to repres ...	1985	18553787
comparisons between cellulase production by aspergillus fumigatus in agitated vessels and in an air-lift fermentor.	aspergillus fumigatus was cultured in disc-turbine-agitated vessels and in an air-lift fermentor. in the agitated vessels the yield of cellulase was reduced when the agitation rate was increased, although extracellular protein levels rose. the enzyme complex itself was shown to be exceptionally stable under conditions similar to those in the agitated vessels, so probably shear damage to the mycelium had occurred, liberating intracellular contents. these appeared to contain an inhibitor that coul ...	1985	18553798
[comparison of three techniques for the enumeration of aspergillus fumigatus spores in the hatcher as part of an epidemiologic survey of aspergillosis risks in hatchery houses].	methods of enumerating aspergillus fumigatus spores on the down of hatching chicks in swabs of hatcher walls and in lungs of hatching chicks were compared under field conditions as part of an official survey of hatchery houses. correlations and agreements between the results were studied. their lack of accuracy is stressed, especially with the swab technique. this impels the aspergillosis risk to be evaluated from the average of the enumerations obtained simultaneously in a set of 10 hatchers.	1985	18766926
study of lignin biotransformation by aspergillus fumigatus and white-rot fungi using (14)c-labeled and unlabeled kraft lignins.	the biodegradation of lignin by fungi was studied in shake flasks using (14)c-labeled kraft lignin and in a deep-tank fermentor using unlabeled kraft lignin. among the fungi screened, a. fumigatus-isolated in our laboratories-was most potent in lignin biotransformation. dialysis-type fermentation, designed to study possible accumulation of low mw lignin-derived products, showed no such accumulation. recalcitrant carbohydrates like mi-crocrystalline cellulose supported higher lignolytic activity ...	1986	18555341
the activity of ketoconazole and itraconazole against aspergillus fumigatus in mixed cultures with macrophages or leukocytes.		1986	3012331
rast-specific ige in nigerian asthmatic patients.	this first study of the pattern of allergen-specific ige in nigerian asthmatic patients in zaria shows a significant percentage with specific ige to dermatophagoides pteronnyssinus, aspergillus fumigatus, and cynodon dactylon (bermuda grass). total serum ige varied in both urban and rural asthmatics with a pattern of low and high levels in all groups.	1986	3484920
corneal biopsy in the diagnosis of keratomycosis.	in two patients, a 55-year-old man and a 49-year-old man, who had fungal keratitis initially undiagnosed by corneal scrapings the condition was successfully diagnosed by corneal biopsy. we compared corneal biopsy specimens and corneal scraping in the diagnosis of keratomycosis in rabbits with experimental bilateral fungal keratitis caused by fusarium solani, aspergillus fumigatus, and candida albicans. corneal scrapings disclosed three specimens (30%) positive for candida, five (50%) for fusariu ...	1986	3485380
pulmonary infection in cystic fibrosis.		1986	3090153
significance of isolation of aspergillus from the respiratory tract in diagnosis of invasive pulmonary aspergillosis. results from a three-year prospective study.	the isolation of aspergillus species from respiratory secretions has been regarded as being of limited usefulness in the antemortem diagnosis of invasive pulmonary aspergillosis. one hundred and eight consecutive patients were evaluated in whom aspergillus species were isolated from respiratory secretions. invasive aspergillosis was not demonstrated in non-immunosuppressed patients or in patients with solid tumors in the absence of neutropenia. lung tissue was examined in 17 patients with leukem ...	1986	3090879
enzyme-linked immunosorbent assay in the detection of specific antibodies against aspergillus in patient sera.	a biotin-avidin linked enzyme immunoassay (balisa) was used to detect aspergillus fumigatus specific antibodies in the sera of patients with aspergillosis. culture filtrate antigens from a strain of a. fumigatus grown in synthetic medium was used in the elisa. this antigen was characterized by various immunochemical methods including isoelectric focusing, two-dimensional electrophoresis and antigen antibody crossed-immunoelectrophoresis. sera from patients having allergic asthma, allergic bronch ...	1986	3094289
selective partitioning of conidia of some penicillium and aspergillus species in aqueous two-phase systems.	conidia of aspergillus fumigatus, aspergillus flavus, aspergillus niger, penicillium brevi-compactum, penicillium frequentans, penicillium spinulosum, and penicillium verrucosum var. cyclopium were subjected to partition at varying ph values in an aqueous two-phase system containing charged polyethylene glycol. in the system, the partition behavior of the conidia of the penicillium species varied when the ph was raised, while the conidia of the aspergillus species seemed unaffected. p. brevi-com ...	1986	3096203
enzyme profile and immunochemical characterization of aspergillus fumigatus antigens.	we have compared the immunochemical characteristics of culture-filtrate antigens (ag) from aspergillus fumigatus extracted in our laboratory with commercially available ags. a total of 20 different preparations were studied for protein and carbohydrate content, presence of endotoxins, mycotoxins, and hemolytic toxins. these extracts were analyzed by two-dimensional electrophoresis for protein components. the immunogenicity of the preparations was determined by rocket electrophoresis with rabbit ...	1986	3097110
pulmonary aspergilloma. results of surgical treatment.	between 1953 and 1984, 53 patients (40 male and 13 female) underwent thoracotomy for treatment of pulmonary aspergilloma. the median age was 58 years (range 4 to 86 years). either underlying lung disease or immunologic risk factors were present in 49 patients (92%). twenty-one patients (31%) had simple aspergilloma and 32 (47%) had complex aspergilloma. the most common indication for operation was an indeterminate mass, hemoptysis, or severe cough. lobectomy, wedge excision, and pneumonectomy we ...	1986	3097424
a partially purified glycoprotein antigen from aspergillus fumigatus.	culture filtrate antigens of aspergillus fumigatus were fractionated by isoelectric focusing using a ph gradient of 4-6.5. three fractions, namely, 18, 19 and 20 were pooled and subjected to immunochemical analysis. it contained a concanavalin a binding glycoprotein. antibody raised against this component was used to prepare an affinity column of igg-sepharose and was used to purify crude culture filtrate antigens. this component produced three precipitin arcs in crossed immunoelectrophoresis us ...	1986	2419255
evaluation of cross-reactions in histoplasma capsulatum serologic tests.	cross-reactivity in histoplasma serologic tests was evaluated by using sera from patients with histoplasmosis and other infections. serum samples from 127 of 134 (95%) patients with histoplasmosis were judged positive by complement fixation tests, and 121 (90%) showed h bands, m bands, or both by immunodiffusion. of these 134 patients, cross-reactions were seen to blastomyces dermatitidis in 53 patients (40%), to coccidioides immitis in 20 patients (16%), and to aspergillus fumigatus in 3 patien ...	1986	2420821
studies on bronchial hyperreactivity, allergic responsiveness, and asthma in rural and urban children of the highlands of papua new guinea.	the prevalence of asthma and allergic responsiveness in rural and urban children of the highlands of papua new guinea was studied. bronchial provocation studies with histamine demonstrated significant bronchial hyperreactivity in 0.5% (1 in 195) rural and 1.7% (1 in 59) urban children, rates which were significantly lower than those observed in corresponding adult populations (7%). urban children demonstrated a higher incidence of skin test reactivity toward dermatophagoides pteronyssinus, asper ...	1986	2420852
cross-reactive polysaccharides from trypanosoma cruzi and fungi (especially dactylium dendroides).	cross-reactivity between fungal and trypanosoma cruzi polysaccharides, owing to common residues of beta-d-galactofuranose, beta-d-galactopyranose, and alpha-d-mannopyranose, was demonstrated by using rabbit immune sera against t. cruzi epimastigotes and sera from patients with chagas' disease. several chagasic (ch) sera precipitated partly purified galactomannans from aspergillus fumigatus and from t. cruzi epimastigotes and also the galactoglucomannan from dactylium dendroides. reaction of one ...	1986	2426444
[development, symptomatology and treatment of "animal" and "plant" parasites of the central nervous system].	eight cases of today rather rare infections of the brain and spinal cord with animal and plant parasites are described. echinococcus was identified in one patient, cysticercus cellulosis in 4 and in 3 patients various fungi (actinomycosis meyerii, aspergillus fumigatus, nokardiose asteroides) were found. prior to the admission to the hospital none of these patients were correctly diagnosed. animal parasites mimicked the symptomatology of a brain tumour or of a space occupying process. if localis ...	1986	2428102
the effect of gliotoxin upon macrophage function.	gliotoxin, a metabolite of the fungus aspergillus fumigatus, inhibited phagocytosis of particulate matter by rodent macrophages. in addition, adherence to plastic surfaces by peritoneal and alveolar rodent macrophages, human peripheral blood monocytes, mouse secondary fibroblasts and l929 cells was differentially inhibited by gliotoxin. electron microscopy which confirmed the inhibition by gliotoxin of phagocytosis of carbon particles by rodent macrophages also revealed gliotoxin-induced morphol ...	1986	2430903
[effect of the components of the medium on aspergillus fumigatus growth and its formation of gliotoxin].		1986	2478869
separation of mycotoxin-containing sources in grain dust and determination of their mycotoxin potential.	two distinct reservoirs of mycotoxins exist in fungal-infected cereal grains--the fungal spores and the spore-free mycelium-substrate matrix. many fungal spores are of respirable size and the mycelium-substrate matrix can be pulverized to form particles of respirable size during routine handling of grain. in order to determine the contribution of each source to the level of mycotoxin contamination of dust, we developed techniques to harvest and separate mycelium-substrate matrices from spores of ...	1986	3709472
fungi on the hair of large mammals in egypt.	the frequency of occurrence of fungi in 260 hair samples of dog, donkey and cow collected from veterinary medicine hospital of assiut university was determined. twenty-three species which belong to fifteen genera were collected. in low frequency, several dermatophytes (chrysosporium, microsporum, arthroderma and trichophyton) were found as well as some other fungal species pathogenic to man and animals (aspergillus fumigatus, stachybotrys chartarum, scopulariopsis brevicaulis and cephalosporium ...	1986	3713793
superinfection by aspergillus fumigatus of a pulmonary lesion caused by mycobacterium xenopi.	a 36-year-old man had a pulmonary infection with a cavity in the right upper lobe caused by mycobacterium xenopi. a superinfection by aspergillus fumigatus occurred 1 year later. both organisms were found in the resected lung specimen. the patient remained asymptomatic after surgery. the superinfection of healed tuberculous lesions by 'atypical' mycobacteria or aspergillus species is well known but the co-existence of the 2 organisms is very rare.	1986	3715986
antifungal activity of ketoconazole with emphasis on zoophilic fungal pathogens.	the antifungal activity of ketoconazole was studied against fungal pathogens associated with various mycotic infections in animals. a concentration of 10 micrograms of ketoconazole/ml of medium proved fungicidal to trichophyton verrucosum. the same concentration acted as a strong fungistat against the majority of the tested dermatophytes. yeasts were generally more sensitive than yeast-like pathogens. ketoconazole also proved fungicidal against pityrosporon canis and strongly inhibitory on crypt ...	1986	3729122
fowl cholera: protection against pasteurella multocida by ribosome-lipopolysaccharide vaccine.	ribosomal protein from aspergillus fumigatus substituted for intact ribosomes in potentiating the immunogenicity of pasteurella multocida lipopolysaccharide. ribosomal protein behaved as a carrier for the lipopolysaccharide. the basic protein methylated albumin, but not protamine sulfate, substituted for ribosomal protein as a carrier for lipopolysaccharide. synthetic single- and double-stranded polynucleotides did not function as an adjuvant to potentiate the immunogenicity of lipopolysaccharid ...	1986	3729887
detection of allergens in mould and mite preparations by a nitrocellulose electroblotting technique.	the allergen composition of moulds (alternaria alternata and aspergillus fumigatus) and mites (dermatophagoides pteronyssinus and dermatophagoides farinae) was studied by electroblotting. the allergens were separated by sds-gpage and transferred to a nitrocellulose membrane by electroblotting. specific ige antibodies directed against mould and mite allergens were utilized as probes to detect the allergens. the bands were localized on the nitrocellulose membrane by means of enzyme- or isotope-lab ...	1986	3733230
cerebral and orbital aspergillus infection due to invasive aspergillosis of ethmoid sinus.	aspergillus fumigatus infection due to primary infection of the ethmoid sinus affected the brain and orbit of an otherwise healthy elderly woman. primary nasal sinus infection may be a chronic process analogous to aspergilloma of the lung: it may, however, assume a locally invasive course and spread to the orbit or brain, as in the case described. this form of aspergillus infection is rare in europe but common in hot dry areas like the sudan. the infection was probably contracted by our patient ...	1986	3734114
[mycoflora of hay and straw].	studies were carried out on hay and straw to establish the composition of mycoflora in them. they were sampled both at the time of harvesting and after storing for four to five months. it was found that the hay and straw were most often inseminated with spores of the fungus species alternaria tenuis, aspergillus fumigatus, mucor hiemalis, penicillium sp., and more rarely with spores of fusarium, chaetomium, scopulariopsis, cladosporium, trichoderma, and stachybotrys alternans. it was demonstrate ...	1986	3739224
the maternal to fetal transfer of immunoglobulins associated with placental lesions in sheep.	in this study we evaluated maternofetal transmission of immunoglobulins in ewes under conditions of altered placental morphology. intravenous injection of human red blood cells was used to induce immunoglobulins in pregnant ewes. the hemagglutination test was used to detect antibody in maternal serum, fetal and placental fluids. placental injury was induced by intravenous inoculation of escherichia coli endotoxin or spores of aspergillus fumigatus into pregnant ewes at days 99 or 100 of gestatio ...	1986	3742359
[aspergillosis with obstruction of the central airways].	two cases are reported of obstruction of central airways by plugs containing aspergillus fumigatus. in one case the obstruction was located in the right stem bronchus and, despite treatment with amphotericin b, the immunocompromised patient died from invasive disseminated aspergillosis. in the other case the obstruction was in the trachea and, since the immune function of the patient was normal, she responded favorably to treatment by amphotericin b.	1986	3775339
circulating antibodies and antigenic cross-reactivity in hendersonula toruloidea and scytalidium hyalinum infections.	twelve (34%) of thirty-five patients with an active infection on the palms or soles caused by hendersonula toruloidea or scytalidium hyalinum were found to have circulating antibodies to these organisms by counter immunoelectrophoresis or immunodiffusion, compared with 9% of uninfected controls. in every instance there was cross-reactivity between the positive patients' sera and the heterologous non-dermatophyte antigen. using crossed and intermediate gel immunoelectrophoresis it was found that ...	1986	3778813
significance of igg antibodies against environmental microbial antigens in a farming population.	a group of dairy farmers studied 6 years earlier in a field survey was re-surveyed for respiratory symptoms, occupational capability and the presence of antibodies against environmental micro-organisms. specific igg antibodies to micropolyspora faeni, thermoactinomyces vulgaris, aspergillus umbrosus and aspergillus fumigatus were measured by elisa from the serum samples obtained in the two surveys. antibody titres remained constant in 70% of both farmers and controls, but where changes took plac ...	1986	3779941
microascus cinereus (emile-weil & gaudin) curzi--a human pathogen?	microascus cinereus is described accompanying aspergillus fumigatus in human lung tissue. the tissue contained a. fumigatus conidiophores and conidia and m. cinereus perithecia, ascospores, annellophores and conidia. the pathogenicity of m. cinereus is discussed.	1986	3808006
[the mycoflora of corn silage].	maize silage was sampled to investigate the mycotic flora in it, the material for testing being taken at the time of preparing the silage as well 4 to 5 months later. it was found that richest mycoflora developed in the superficial layers of the silage mass, chiefly constituted by fungus species of the aspergillus, penicillium, alternaria, and mucor genera. it was also established that aspergillus fumigatus was the most frequently isolated species. it is believed that the development of the moul ...	1986	3811222
[two resected cases of pulmonary aspergillosis coincidentally accompanied by a solid tumor].		1986	3543449
synthesis and structure-activity relationships of naftifine-related allylamine antimycotics.	naftifine (1) is the first representative of the new antifungal allylamine derivatives. its biological activity is strictly bound to specific structural requirements that are unrelated to those of known antifungals. a tertiary allylamine function seems to be a prerequisite for activity against fungi. by systematic variation of the individual structural elements in 1, detailed structure-activity relationships are defined in which the phenyl ring is the structural feature permitting the widest var ...	1986	3510297
head and neck aspergillosis in patients undergoing bone marrow transplantation. report of four cases and review of the literature.	aspergillus infection can be a major cause of morbidity in immunocompromised patients, especially when there is pulmonary involvement. diagnosis of aspergillosis is often complicated by the varied clinical presentation and compromised medical status of such patients. four cases of head and neck aspergillus infections in bone marrow transplant patients are presented. involvement predominantly was limited to the oral cavity and/or sinuses, and in one case, the ear. two cases were successfully mana ...	1986	3510710
[mycotic placentitis in cattle].	two cases of bovine mycotic placentitis are reported. the two cows did not show any clinical symptoms before or during premature delivery of two clinically normal calves. in both placentas a severe placentitis accompanied by extensive necrosis and a large number of hyphae was present in two placentas. aspergillus fumigatus was isolated in one case. the literature on bovine mycotic placentitis is reviewed in the discussion.	1986	3511563
studies on the association of aspergillus fumigatus with ocular infections in animals.	the occurrence and aetiological significance of aspergillus fumigatus, an opportunistic pathogen, have been studied in 93 animals with various ophthalmological problems. a total of 93 eye swabs collected from 35 mules, 26 dogs, 13 fowl, 11 cattle, five buffaloes and three camels were investigated mycologically for the presence of a fumigatus. the pathogen was isolated in pure and heavy growth from the swabs from two dogs, one bull, one mule and one fowl. the fungus was also demonstrated directly ...	1986	3511605
spinal arachnoiditis due to aspergillus meningitis in a previously healthy patient.	a 30-year-old, previously healthy, non-addicted man presented with a chronic spinal meningitis complicated by arachnoiditis and spinal cord compression. biopsy showed a chronic granulomatous leptomeningitis, in which some cells contained branching septate organisms that were immunostained with an antiserum to aspergillus fumigatus. precipitins to a. fumigatus were detected in cerebrospinal fluid (csf), but not in blood, and aspergillus infection was apparently restricted to the leptomeninges. cl ...	1986	3512781
binding of c-reactive protein to aspergillus fumigatus fractions.	calcium-dependent binding of c-reactive protein (crp) to aspergillus fumigatus was determined by enzyme-linked immunosorbent assay. a homogenate of young hyphae was fractionated by hydrophobic interaction chromatography followed by gel filtration. high crp-binding activity was found in a fraction of mol. wt c. 500,000 which was characterised by strong binding to the hydrophobic column. three fractions of less conspicuous crp-binding activity were identified (c. 500 000, 150 000 and 150 000-50 00 ...	1986	3512843
rapid visualization of three common fungi using fluorescein-conjugated lectins.	the feasibility of using fluorescein-conjugated lectins to visualize and differentiate three fungi commonly involved in ophthalmic mycoses was evaluated. using a panel of fluorescein-conjugated lectins, candida albicans, aspergillus fumigatus, and fusarium solani were rapidly and reproducibly visualized in in vitro culture isolates, as well as in tissue samples and fixed histopathologic specimens taken from experimental mycoses. additionally, aspergillus and fusarium were consistently differenti ...	1986	3514531
histopathologic and antibody responses of rabbits exposed to aerosols containing spores of aspergillus fumigatus: comparison of single and multiple exposures.	resistance to pulmonary aspergillosis was studied in groups of rabbits exposed to aerosolized spores of aspergillus fumigatus for 15 minutes on successive days for a total of 10, 7, or 4 exposures or a single exposure. the results of the study demonstrated that exposure of rabbits to spores for 15 minutes on 10 successive days did not result in an accumulation of viable spores in excess of those present in the lungs of rabbits exposed a single time. the tissue response in the lungs of the rabbit ...	1986	3516034
allergic bronchopulmonary aspergillosis. natural history and classification of early disease by serologic and roentgenographic studies.	eighty-four patients with allergic bronchopulmonary aspergillosis (abpa) were evaluated for a total of 294 patient-years with a mean observation period of 3.7 years and classified by the stage of abpa. the largest percentage of patients were in the stage iv (corticosteroid-dependent asthma stage) group. the next largest percentage were in the stage v (fibrotic, end-stage lung disease) group. of the latter 24 patients, eight had died. in addition, we describe 13 patients with all serologic charac ...	1986	3516103
osteomyelitis and arthritis associated with aspergillus fumigatus in a dog.		1986	3516132
[inactivation of the fungus aspergillus fumigatus].		1986	3516632
serum fungistatic and fungicidal activity in volunteers receiving antifungal agents.	the serum levels of two imidazoles, ketoconazole and bayer n7133, when administered alone or in combination with rifampin, were measured in volunteers and the serum antifungal activity was determined against various fungal strains. serum levels were measured by high pressure liquid chromatography and correlated with serum fungistatic activity as determined in microtiter plates. ketoconazole showed a wide range of serum levels, suggesting individual variations. the addition of two doses of rifamp ...	1986	3516681
inhibition of cellular immunity by products of aspergillus fumigatus.	a fraction, fb, from extracts of aspergillus fumigatus which previously was found to display a high degree of cellular hypersensitivity in skin tests in sensitized guinea-pigs but a low ability to affect transformation of lymphocytes in vitro, was studied. this fraction was found to be capable of inhibiting lymphocyte transformation induced in vitro by tuberculin and by t and b cell mitogens. the inhibitory properties of fb on lymphocyte activation were heat labile, destroyed by proteolysis, and ...	1986	3517279
pulmonary aspergilloma. case reports from malaysia.	pulmonary aspergilloma is by no means uncommon in malaysia. the lack of documentation of its occurrence in malaysia, is mainly due to the lack of clinical awareness, and the absence of facilities for the proper diagnosis of the infection.	1986	3520330
[a case of cerebral aspergilloma following radical operation of a cerebral aneurysm].	a case of aspergillotic abscess with granuloma is reported. a 45-year-old man was admitted to our hospital on apr. 10, 1984 due to the rupture of an aneurysm of the anterior communicating artery. neck clipping of the aneurysm was proposed on apr. 12, 1984, but was not performed because of cardiac arrest with unknown etiology during the operation. neck clipping was performed on apr. 23, 1984. antibiotic therapy was prolonged for about three weeks. about 6 months after surgery, he was readmitted o ...	1986	3520367
[pulmonary aspergillosis in a child with septic granulomatosis].		1986	3520537
[limited aspergillosis of the bronchi].		1986	3520544
[the dot immunobinding assay for specific ige and igg antibodies to dermatophagoides farinae].		1986	3521548
class-specific antibody determination against aspergillus fumigatus by means of the enzyme-linked immunosorbent assay. iii. comparative study: igg, iga, igm elisa titers, precipitating antibodies and ige binding after fractionation of the antigen.	igg, iga and igm elisa antibody titers against aspergillus fumigatus were elevated in sera of patients with aspergilloma and allergic bronchopulmonary aspergillosis (abpa), showing higher titers for the igg antibodies compared with the iga and igm antibodies. no differences were found between titers of identical antibody classes in the two groups of sera. igg and iga elisa titers were highly specific whereas igm elisa showed more unspecific binding of igm antibodies. antibodies, as measured by e ...	1986	3522437
antigens and allergens of aspergillus fumigatus. ii. their further identification and partial characterization of a major allergen (ag 3).	these further studies demonstrated that most antigens and allergens detected by allergic bronchopulmonary aspergillosis (abpa) sera were recognized by rabbit antiserum, and a major allergenic component, ag 3, as previously demonstrated by self-crossed radioimmunoelectrophoresis, was identified in the conventional system. affinocrossed immunoelectrophoresis with concanavalin a indicated that for the patients with abpa, although the major antigens contained alpha-d-manno- or alpha-d-glucopyranosid ...	1986	3522706
antigens and allergens of aspergillus fumigatus. i. characterization by quantitative immunoelectrophoretic techniques.	both the igg (precipitinogen) and ige combining components of aspergillus fumigatus have been studied by quantitative immunoelectrophoretic techniques with sera of patients with allergic bronchopulmonary aspergillosis and specific rabbit antisera. considerable batch-to-batch variation in biochemical properties and in immunologic (both antigenic and allergenic) activity was revealed in identically prepared culture-filtrate antigens of the same strain of a. fumigatus. profiles of crossed immunoele ...	1986	3522708
serum iga antibodies to aspergillus fumigatus in various stages of allergic bronchopulmonary aspergillosis.	with double antibody elisa, serum iga antibodies against aspergillus fumigatus (af) were measured from patients in stages i through v allergic bronchopulmonary aspergillosis (abpa). all sera from patients with abpa demonstrated considerably greater values for iga-af than sera from nonatopic subjects. when this sera was compared with sera from patients with asthma and immediate cutaneous reactivity to aspergillus, the present studies documented markedly elevated serum iga-af in the acute, exacerb ...	1986	3522709
treatment of canine nasal aspergillosis with systemic ketoconazole and topical enilconazole.		1986	3523960
[mycotic lesions of the bronchopulmonary system (experimental study)].		1986	3525333
in vitro killing of spores and hyphae of aspergillus fumigatus and rhizopus oryzae by rabbit neutrophil cationic peptides and bronchoalveolar macrophages.	the fungicidal activity of six rabbit neutrophil cationic peptides (np) against resting (dormant) spores, preincubated (swollen) spores, and hyphae of aspergillus fumigatus and rhizopus oryzae was examined. whereas resting spores of both fungi were totally resistant to killing by up to 500 micrograms of np/ml, preincubated spores and hyphae were sensitive, under optimal conditions, to less than 1 microgram /ml. np-1 and np-2, also found in rabbit bronchoalveolar macrophages, were the most active ...	1986	3525696
aspergillus lung disease.		1986	3525822
[chronic necrotizing pulmonary aspergillosis].		1986	3526022
[empyema caused by aspergillus. apropos of 3 cases].		1986	3526429
fatal aspergillus pneumonia in chronic granulomatous disease.	a 20-year-old man died from aspergillus pneumonia three weeks after heavy exposure to grain dust. lung biopsy and autopsy demonstrated a distinctive form of suppurating granulomatous bronchopneumonia caused by aspergillus fumigatus, which was the sole agent cultured from the tissue. the liver and lymph nodes contained pigmented lipid histiocytes characteristic of chronic granulomatous disease, and subsequently both of the patient's brothers were found to have x-linked chronic granulomatous disea ...	1986	3526863
development of a sandwich elisa to detect igg and igg sub-class antibodies specific for a major antigen (ag 7) of aspergillus fumigatus.	a sandwich elisa has been developed, using an affinity purified monospecific antiserum as a capture antibody, to detect specific igg and igg sub-classes to a major antigen (ag 7) of aspergillus fumigatus in the sera of patients with allergic bronchopulmonary aspergillosis (abpa). significantly elevated levels of specific igg to ag 7 were detected in 97% of abpa sera tested, as compared to control sera and to sera from a. fumigatus skin-prick test positive individuals. igg sub-class antibody leve ...	1986	3527473
primary pulmonary aspergilloma: case report and review of the literature.	we describe a patient with acute leukemia who developed a primary aspergilloma. a combination of surgical resection and parenteral amphotericin b cured the lesion. the pathophysiology, diagnosis and treatment of primary pulmonary aspergilloma are reviewed.	1986	3528050
[mycetoma of the lungs].	mycetomas of the lungs are, as a rule, localized in the upper (62), medial (22) and very rarely (8) in the lower portions of the lungs. most frequent localization is the right lung. operations were fulfilled on 68 of 92 patients. surgery was shown to be the most effective method of treatment of patients with lung mycetomas. it results in complete and clinical recovery.	1986	3529583
aspergillosis--clinical aspects.	allergic bronchopulmonary aspergillosis (abpa) is much more common than originally suspected, can have its onset in childhood and remain undiagnosed for years or decades, at which time it presents in a patient with end-stage fibrotic lung disease. in other patients, abpa may cause a finite number of roentgenographic lesions and not be associated with chronic sputum production or widespread bronchiectasis. clinical symptoms range from the patient being asymptomatic with a new roentgenographic inf ...	1986	3532632
aspergillus fumigatus antigens used in the serodiagnosis of aspergillosis.	the diagnostic antigens in aspergillus serology are mycelial or culture filtrate concentrates. recent work has been directed towards the separation of defined, reproducible products of greater sensitivity and specificity than crude extracts. conventional methods of separation have been used; they include fractional precipitation, detergent extraction, chromatography, and preparative isoelectric focusing. selective staining of the separated fractions has shown many of the more reactive components ...	1986	3532633
allergens of aspergillus fumigatus: identification and characterization of components combining with specific ige antibody.	aspergillus fumigatus-specific ige antibodies, mediating immediate type hypersensitivity reactions are found in a proportion (16-26%) of patients with asthma and their presence is a criterion for diagnosis of allergic bronchopulmonary aspergillosis (abpa). sensitive radiolabelled or enzyme-linked immunoassays are required for detection/quantification of these antibodies in patient's serum, and the fungal components, allergens, to which they combine, may be identified in crossed radio-immunoelect ...	1986	3532634
recent statistical survey of visceral aspergillosis in japan, and experimental studies on the pathogenicity of aspergillus fumigatus in rabbits.	a statistical survey of visceral aspergillosis in japanese pathological autopsies (1972-1981) was carried out. it was found that the infection has increased remarkably in recent years, and the results obtained were briefly described. pathogenicity of several strains of aspergillus fumigatus in rabbits was studied on three occasions in the past 31 years. the pathogenicity appeared to be unchanged even after long subcultures for 13 years, while lowered pathogenicity was noted with long-term subcul ...	1986	3532636
cerebral aspergillosis and encephalomeningocele.	a case of massive cerebral aspergillosis complicating a frontal encephalomeningocele is reported. the etiology and pathogenesis are discussed and the diagnosis, symptoms, and treatment possibilities reviewed.	1986	3536091
[clinical course and diagnosis of chronic aspergillosis of the bronchi and lungs].		1986	3536674
inhibition of complement by culture supernatants of aspergillus fumigatus.	aspergillus fumigatus produced a water-soluble extracellular material that inhibited the opsonization of fungal cells by normal human serum. blastospores of cryptococcus neoformans and conidia from a. fumigatus or aspergillus niger were used as fungal targets for ingestion by human monocytes. the opsonic inhibitor from a. fumigatus was found to decrease binding of complement component c3b to fungal surfaces by selectively interfering with activation of the alternative complement pathway. inhibit ...	1986	3537152
successful management of primary invasive pulmonary aspergillosis.	we present the successful recovery of an immunocompetent patient with invasive pulmonary aspergillosis in the absence of any previous detectable lung damage or underlying systemic disease.	1986	3539169
characterization of a major antigenic component of aspergillus fumigatus.	a component of aspergillus fumigatus, ag 7, previously identified as a major antigen for patients with allergic bronchopulmonary aspergillosis, has been isolated by gel filtration and further purified by affinity chromatography using monospecific antiserum. the antigen, which binds both specific igg and ige antibodies, was shown to be a high molecular weight, 150-200 kd, heat-stable glycoprotein, which binds to concanavalin a, suggesting the presence of alpha-d mannopyrannoside, or alpha-d gluco ...	1986	3539424
allergic bronchopulmonary aspergillosis in asthmatic patients presenting with allergic rhinitis.	the early diagnosis of allergic bronchopulmonary aspergillosis (abpa) is is important because it may allow prevention of evolution to the fibrotic lung disease stage (stage v). in two immunotherapy studies of 62 patients recruited for allergic rhinitis, we conducted studies to exclude abpa in any subject with asthma and positive immediate skin reactivity to aspergillus fumigatus. the evaluation included serum precipitating antibodies against a. fumigatus, total serum ige, blood eosinophilia, ser ...	1986	3949407
[aspergillus pulmonary infection. diagnosis using transthoracic aspiration puncture].		1986	3540481
transepithelial elimination in cutaneous aspergillosis.		1986	3543075
computed tomography of renal aspergillosis.	the ct and sonographic findings of a case of localized, invasive aspergillosis of the kidney are presented.	1987	3543078
cystic fibrosis in adolescents and adults.	three hundred and sixteen patients with cystic fibrosis were seen at the brompton hospital during 1965-83; 178 (56.3%) of them were male and 136 female, and their ages ranged from 12 to 51 years. most patients presented in infancy with respiratory symptoms and malabsorption, but 19 (6%) were diagnosed in adult life, three in their 30s. pulmonary disease was almost universal (99.7%), being responsible for 97% of all deaths and three quarters of hospital admissions. all patients had developed a pr ...	1987	3438896
precipitins against microbes in mouldy hay in the sera of farmers with farmer's lung or chronic bronchitis and of healthy farmers.	the aim of this study was to determine which precipitins against four antigens in 2,440 farmers are associated with the occurrence of farmer's lung (fl). the antigen panel consisted of mycelial antigens of micropolyspora faeni, thermoactinomyces vulgaris, aspergillus fumigatus and aspergillus umbrosus. as reference groups we used healthy farmers and those with chronic bronchitis. for the occurrence of precipitins against the four antigens there was a statistically significant difference between ...	1987	3478211
in-hospital source of airborne penicillium species spores.	between 16 july and 1 october 1984, prospectively monitored corridor air samples from a bone marrow transplant station revealed a marked increase in airborne thermotolerant penicillium spores. simultaneous cultures of outside air showed lower spore counts, which were unchanged before, during, and after the corridor outburst, establishing that the source was within the hospital. although the corridor was equipped with recirculating high-efficiency particulate air filtration units which provided 1 ...	1987	3539981
aspergillus fumigatus-specific antibodies in allergic bronchopulmonary aspergillosis and aspergilloma: evidence for a polyclonal antibody response.	patients with the aspergillus-induced diseases allergic bronchopulmonary aspergillosis (abpa), aspergilloma (fungus ball), and aspergillus skin test-positive asthma were differentiated immunologically by radioimmunoassay based on their total immunoglobulin e (ige) and aspergillus fumigatus-specific ige levels. in this study, a new, highly sensitive biotin-avidin-linked immunosorbent assay was used to evaluate a. fumigatus-specific antibodies of all immunoglobulin classes. studied populations inc ...	1987	3539998
serodiagnosis of invasive aspergillosis in patients with hematologic malignancy: validation of the aspergillus fumigatus antigen radioimmunoassay.	six hundred sixteen sera from 79 hematology patients admitted on 152 occasions were analyzed for validation of the aspergillus fumigatus antigen radioimmunoassay (ria). invasive aspergillosis developed on 24 admissions of 22 patients. maximal antigenic activity was significantly higher in patients with invasive aspergillosis than in controls (p less than .0005). at the level of antigenic activity selected as the cutoff value, the sensitivity of the ria was 74%, the specificity 90%, the positive ...	1987	3540137