Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| isolation and analysis of the acetate regulatory gene, facb, from aspergillus nidulans. | the facb gene of aspergillus nidulans is thought to be involved in acetate induction of enzymes required for acetate utilization and of the acetamidase encoded by the multiply regulated amds gene. in addition, some evidence suggests that the facb gene has a structural as well as a regulatory role in acetate metabolism. the facb gene was cloned from a cosmid library by complementation of the facb101 loss-of-function mutation. transformants receiving multiple copies of facb displayed stronger grow ... | 1989 | 2685573 |
| aspergillus findings in aids patients suffering from cryptococcosis. | because of the known pathogenicity of cryptococcus neoformans and of aspergilli depending on defined but different immunodeficiencies of the host, the evaluation of their simultaneous cultural detection in specimens of the respiratory tract of aids patients is of epidemiological, diagnostic, pathogenetic and therapeutic interest. in 10 out of 15 aids patients the following species of the genus aspergillus could be isolated either once or repeatedly during the course of cr. neoformans infections ... | 1989 | 2685598 |
| a gene transfer system based on the homologous pyrg gene and efficient expression of bacterial genes in aspergillus oryzae. | a homologous transformation system for aspergillus oryzae is described. the system is based on an a. oryzae strain deficient in orotidine-5'-phosphate decarboxylase (pyrg) and the vector pao4-2, which contains a functional a. oryzae pyrg gene as selection marker. transformation of the a. oryzae pyrg mutant with circular pao4-2 resulted in the appearance of pyr+ transformants at a frequency of up to 20 per micrograms of dna, whereas with linear pao4-2 up to 200 transformants per micrograms dna we ... | 1989 | 2688930 |
| rhizoxin resistant mutants with an altered beta-tubulin gene in aspergillus nidulans. | rhizoxin and ansamitocin p-3 (a maytansinoid compound), potent inhibitors of mammalian brain tubulin assembly, inhibit growth of a variety of fungi including aspergillus nidulans. mutants of a. nidulans, bena10 which is a benomyl resistant beta-tubulin gene mutant and tuba1 which is a benomyl supersensitive alpha-tubulin gene mutant, were both sensitive to rhizoxin and ansamitocin p-3 to the same extent as wild-type strains. we isolated 18 rhizoxin resistant mutants of a. nidulans. all of these ... | 1989 | 2691873 |
| cloning of the crea gene from aspergillus nidulans: a gene involved in carbon catabolite repression. | the crea gene from a. nidulans has been cloned by complementation of a non-revertable mutant allele using a genomic library and marker rescue techniques. the rescued sequence was subcloned and a 2.3 kb fragment identified which complements several crea mutant alleles. northern analyses showed that crea encodes a transcript of approximately 1.8 kb in length and that the levels of this transcript varied by up to two fold depending on the carbon source. transformants containing more than two extra ... | 1989 | 2673558 |
| investigation of the het genes that control heterokaryon incompatibility between members of heterokaryon-compatibility (h-c) groups a and g1 of aspergillus nidulans. | a chromosome assay method was used to determine the heterokaryon compatibility relationships between strains belonging to heterokaryon-compatibility (h-c) groups a and g1 of aspergillus nidulans. a hybrid strain (rd15) was isolated following protoplast fusion of strains 65-5 (h-ca) and 7-141 (h-cg1). the morphology of rd15 was severely abnormal compared to diploid strains of a. nidulans produced from heterokaryon-compatible haploid parents. inocula of rd15 were induced to haploidize on medium co ... | 1990 | 2283501 |
| identification of an amino acid substitution in the bena, beta-tubulin gene of aspergillus nidulans that confers thiabendazole resistance and benomyl supersensitivity. | we are using molecular genetic techniques to identify sites of interaction of beta-tubulin with benzimidizole anti-microtubule agents. we have developed a marker-rescue technique for cloning mutant alleles of the bena, beta-tubulin gene of aspergillus nidulans and have used the technique to clone two mutant bena alleles, bena16 and bena19. these are the only a. nidulans alleles known to confer resistance to the benzimidazole antimicrotubule agent thiabendazole and supersensitivity to other benzi ... | 1990 | 2257633 |
| the identification of mutations in aspergillus nidulans that lead to increased levels of adhii. | there are at least three alcohol dehydrogenases in aspergillus nidulans. adhii has been observed in polyacrylamide gels stained for adh activity but, unlike adhi and adhiii, no physiological function has been attributed to it. this paper describes mutations that have been isolated from strains carrying a deletion in the structural gene for adhi (alca) and its adjacent positively-acting regulatory gene (alcr) that restore some ability to utilise ethanol as a carbon source. the mutations map at th ... | 1990 | 2245474 |
| genetic analysis of amds transformants of aspergillus niger and their use in chromosome mapping. | the aspergillus nidulans gene coding for acetamidase (amds) was introduced into a. niger by transformation. twelve amd+ transformants were analysed genetically. the amds inserts were located in seven different linkage groups. in each transformant the plasmid was integrated in only a single chromosome. our (non-transformed) a. niger strains do not grow on acetamide and are more resistant to fluoroacetamide than the transformants. diploids hemizygous for the amds insert have the amd+ phenotype. we ... | 1990 | 2274031 |
| alcohol dehydrogenase iii in aspergillus nidulans is anaerobically induced and post-transcriptionally regulated. | an alcohol dehydrogenase was shown to be induced in aspergillus nidulans by periods of anaerobic stress. this alcohol dehydrogenase was shown to correspond to the previously described cryptic enzyme, alcohol dehydrogenase iii (mcknight et al. 1985), by analysis of a mutation in the structural gene of alcohol dehydrogenase iii, alcc, created by gene disruption. survival tests on agar plates showed that this enzyme is required for long-term survival under anaerobic conditions. northern blot analys ... | 1990 | 2274033 |
| localization of pyruvate carboxylase in organic acid-producing aspergillus strains. | the localization of pyruvate carboxylase (cytosolic or mitochondrial) was studied in nine different aspergillus species (14 strains). in some species (a. aculeatus, a. flavus, a. foetidus, a. nidulans, a. ochraceus, and a. sojae), the pyruvate carboxylase activity could be detected only in the cytosolic fraction of the cells. pyruvate carboxylase has been found only in the mitochondrial fraction of two strains of aspergillus wentii. in aspergillus oryzae and in five strains of aspergillus niger, ... | 1990 | 2383004 |
| high-efficiency transformation system for the biocontrol agents, trichoderma spp. | we have developed an efficient transformation system based on the use of polyethylene glycol and cacl2 for the biocontrol agents, trichoderma spp. transformation was obtained with the plasmid pan7-1, carrying a bacterial hygromycin-resistance gene as a selectable marker, under the control of aspergillus nidulans heterologous expression signals. the system described here yielded 200-800 transformants per microgram of dna. transformants contained several copies of the plasmid integrated into their ... | 1990 | 2388561 |
| survey of the mycoflora and mycotoxins of cotton seeds and cotton seed products in egypt. | thirty-nine species and 16 fungal genera were isolated from egyptian cotton seeds, cotton seed meal and cotton seed cake on 1% glucose-czapek's agar medium incubated at 28 degrees c. aspergillus was the most frequent genus and it emerged in 87-100% of the samples contributing 70-98% of total fungi in the three substrates tested. the most common species were a. niger, a. flavus, a. fumigatus, a. terreus and rhizopus stolonifer; a. niger, a. fumigatus and penicillium corylophilum; and a. niger, a. ... | 1990 | 2388680 |
| purification, cloning, and primary structure of an enantiomer-selective amidase from brevibacterium sp. strain r312: structural evidence for genetic coupling with nitrile hydratase. | an enantiomer-selective amidase active on several 2-aryl and 2-aryloxy propionamides was identified and purified from brevibacterium sp. strain r312. oligonucleotide probes were designed from limited peptide sequence information and were used to clone the corresponding gene, named amda. highly significant homologies were found at the amino acid level between the deduced sequence of the enantiomer-selective amidase and the sequences of known amidases such as indoleacetamide hydrolases from pseudo ... | 1990 | 2254253 |
| studies on the mycoflora of aswan high dam lake, egypt: monthly variations. | fifty-one species and one variety appertaining to twenty one genera of mesophilic fungi were recovered from the monthly samples of marginal water (44 species, 1 variety and 18 genera) and submerged mud (78 species, 1 variety and 30 genera) of aswan high dam lake during the period from july 1985 to december 1986. the most common species were aspergillus fumigatus, a. flavus, a. terreus, a. niger and penicillium funiculosum. the highest fungal populations were almost detected either in october, in ... | 1990 | 2352135 |
| solubilisation of a cell wall bound invertase in aspergillus nidulans. | aspergillus nidulans produces an extracellular invertase when incubated in the presence of sucrose and about half of the activity produced was found to be associated with the mycelium. sixty percent of this mycelial invertase could be solubilised by simple mechanical disruption. among the agents tested for solubilisation of invertase, proteinase k and dithiothreitol were the most effective. | 1990 | 2272493 |
| complementation of the aspergillus nidulans arg b1 mutation by ornithine transcarbamylase cdna from rat liver. | an aspergillus nidulans strain which is deficient in ornithine transcarbamylase due to the arg b1 mutation was transformed with a plasmid containing the ornithine transcarbamylase cdna from rat liver under the control of the amd s promoter. stable transformants were obtained by selection on arginine free medium indicating complementation of the arg b mutation. proof of expression of the rat enzyme in transformants was obtained by immunoprecipitation of all ornithine transcarbamylase activity fro ... | 1990 | 2189407 |
| molecular basis for determining the sensitivity of eucaryotes to the antimitotic drug rhizoxin. | rhizoxin, an antibiotic, exhibits potent anti-mitotic activity against most eucaryotic cells including those of higher vertebrates, plants and fungi by binding to beta-tubulin. the bena gene of three independently isolated rhizoxin-resistant (rhir) mutants of aspergillus nidulans was cloned, sequenced and compared with that of the wild-type, rhizoxin-sensitive (rhis) strain. in all three rhir mutants, the aac codon for asn-100 of the bena beta-tubulin gene was altered to atc, coding for ile. seq ... | 1990 | 2274023 |
| genetic analysis of aspergillus niger: isolation of chlorate resistance mutants, their use in mitotic mapping and evidence for an eighth linkage group. | this paper describes the use of chlorate resistant mutants in genetic analysis of aspergillus niger. the isolated mutants could be divided into three phenotypic classes on the basis of nitrogen utilization. these were designated nia, nir and cnx as for aspergillus nidulans. all mutations were recessive to their wild-type allele in heterokaryons as well as in heterozygous diploids. the mutations belong to nine different complementation groups. in addition a complex overlapping complementation gro ... | 1990 | 2381424 |
| the complete dna sequence of the mitochondrial genome of podospora anserina. | the complete 94,192 bp sequence of the mitochondrial genome from race s of podospora anserina is presented (1 kb = 10(3) base pairs). three regions unique to race a are also presented bringing the size of this genome to 100,314 bp. race s contains 31 group i introns (33 in race a) and 2 group ii introns (3 in race a). analysis shows that the group i introns can be categorized according to families both with regard to secondary structure and their open reading frames. all identified genes are tra ... | 1990 | 2357736 |
| expression and secretion in aspergillus nidulans and aspergillus niger of a cell surface glycoprotein from the cattle tick, boophilus microplus, by using the fungal amds promoter system. | a cell surface glycoprotein (bm86) from cells of the digestive tract of the cattle tick boophilus microplus, which has been shown to elicit a protective immunological response in vaccinated cattle, was expressed and secreted in the filamentous fungi aspergillus nidulans and aspergillus niger by using the fungal amds promoter system. the cloned gene coded for the bm86 secretory signal and all of the bm86 mature polypeptide except for the hydrophobic carboxy-terminal segment. high levels of bm86 m ... | 1990 | 2275533 |
| an electrophoretic karyotype of aspergillus niger. | an electrophoretic karyotype of aspergillus niger was obtained using contour-clamped homogeneous electric field (chef) gel electrophoresis. chromosome-sized dna was separated into four bands. seven of the eight linkage groups could be correlated with specific chromosomal bands. for this purpose dna preparations from seven transformant strains of a. niger each carrying the heterologous amds gene of aspergillus nidulans on a different chromosome were analysed. some of the assignments were confirme ... | 1990 | 2277644 |
| development of a homologous transformation system for aspergillus parasiticus with the gene encoding nitrate reductase. | the nitrate reductase structural gene (niad) and an niad mutant strain were isolated from aspergillus parasiticus and used to develop a homologous transformation system. a transformation frequency of 110 to 120 transformants per microgram linear dna was obtained with the 10.9 kb plasmid psl82, which contained the niad gene of a. parasiticus. plasmid psl82 was also capable of complementing aspergillus nidulans fgsc a691, a niad mutant, though at lower frequencies. southern hybridization analyses ... | 1990 | 2277647 |
| purification and properties of nadp(+)-dependent glycerol dehydrogenases from aspergillus nidulans and a. niger. | glycerol dehydrogenase, nadp(+)-specific (ec 1.1.1.72), was purified from mycelium of aspergillus nidulans and aspergillus niger using different purification procedures. both enzymes had an mr of approximately 38,000 and were immunologically cross-reactive, but had different amino acid compositions and isoelectric points. for both enzymes, the substrate specificity was limited to glycerol and erythritol for the oxidative reaction and to dihydroxyacetone (dha), diacetyl, methylglyoxal, erythrose ... | 1990 | 2200840 |
| transformation of the rice blast fungus magnaporthe grisea to hygromycin b resistance. | low frequency, integrative transformation of three fertile hermaphroditic strains of magnaporthe grisea has been achieved using plasmid pan7-1 and cosmid pan7-2, which contain an escherichia coli hygromycin b phosphotransferase gene linked to aspergillus nidulans regulatory sequences. | 1990 | 2357737 |
| nucleases in the autolysis of filamentous fungi. | rnase and dnase activities were studied in seven fungi of the subdivisions ascomycotina, zygomycotina and basidiomycotina during their autolysis, and extracellular and intracellular rnase and dnase were found. rnase specific activity reached higher levels than dnase specific activity in the culture liquid and mycelial extract, except in aspergillus nidulans. generally maximal rnase specific activities were observed at the onset of autolysis in the culture liquid. in the mycelial extract an incre ... | 1990 | 2379813 |
| botran and bleomycin induce crossing-over, and bleomycin also increases aneuploidy in diploid strains of aspergillus. | both bleomycin, an antineoplastic drug, and botran (2,6-dichloro-4-nitro-aniline), a fungicide, are known to inhibit growth and induce genetic segregation in diploid tester strains of aspergillus nidulans when present in agar media. to identify primary effects, samples of induced apparent crossover types were analysed in detail. for both compounds, coincident and consecutive events of mitotic crossing-over were found to be very frequent and such events showed a random distribution among isolated ... | 1990 | 1692105 |
| a case of cerebral aspergillosis caused by aspergillus nidulans. clinical, pathologic and mycologic identifications. | a case of cerebral aspergillosis is reported, the presenting symptom was numbness of right face, which worsened after one year. ct-scan showed two enhanced low-density patches in the anterior and basal parts of right temporal lobe. during operation, an abscess in the deep part of right temporal lobe was revealed. the patient gradually felt amaurosis and oculomotor palsy of right eye. about six months later, she died from intracranial hypertension. biopsy, as well as autopsy findings suggested fu ... | 1990 | 2119968 |
| functional expression of 8-hydroxy-5-deazaflavin-dependent dna photolyase from anacystis nidulans in streptomyces coelicolor. | the gene encoding anacystis nidulans 5-deazaflavin-dependent photolyase (phr) was inserted into the streptomyces vector pij385 to form a transcriptional fusion with the neomycin resistance (aph) gene. the resulting plasmid, panpl, was introduced into streptomyces coelicolor, a host which exhibits no detectable photolyase activity and provides 5-deazaflavins. transformants expressed functional photolyase and could be cultured at much higher cell densities than a. nidulans. a two-step affinity pro ... | 1990 | 2120199 |
| analysis of delta-l-alpha-aminoadipyl-l-cysteinyl-d-valine by ion chromatography and pulsed amperometric detection. | a novel high-performance liquid chromatography (hplc) method is presented for the detection and trace level determination of the tripeptide delta-l-alpha-aminoadipyl-l-cysteinyl-d-valine (acv). the tripeptide, an intermediate in penicillin production, is derived from fungal fermentation. the technique relies on ion-exchange separation of the tripeptide on an anion-exchange column followed by detection by reduction on a gold electrode using pulsed amperometry. the sensitivity of direct determinat ... | 1990 | 2120274 |
| spatial and biological characterisation of the complete quinic acid utilisation gene cluster in aspergillus nidulans. | heterologous probing of restriction digests of chromosomal dna from aspergillus nidulans with radioactively labelled probes encoding dehydroshikimate dehydratase (qa-4) and a repressor gene (qa1-s) from neurospora crassa revealed a pattern of hybridisation inconsistent with an equivalent single copy of each gene in a. nidulans. screening of size-selected and total genome a. nidulans dna libraries allowed the isolation of four unique classes of sequence, two of which hybridised to the qa-4 probe, ... | 1990 | 2175387 |
| n-acetyl-6-hydroxytryptophan oxidase, a developmentally controlled phenol oxidase from aspergillus nidulans. | we have purified a specific phenol oxidase which is produced during conidiophore development in the fungus aspergillus nidulans. two active forms (a and b) have molecular masses of 50 and 48 kda respectively; they have identical n-termini (24 residues). we have analysed the metal ion content of the b form; it is unusual in consisting of one zinc and two copper atoms per molecule. a temperature-sensitive mutant (ivob192) produces a thermolabile enzyme, implying that ivob is the structural locus. ... | 1990 | 2126551 |
| the aspergillus nidulans npea locus consists of three contiguous genes required for penicillin biosynthesis. | clones of aspergillus nidulans genomic dna spanning 20 kb have been isolated and shown by a combination of classical and molecular genetic means to represent the npea locus, previously found to be one of four loci (npea, npeb, npec and nped) involved in the synthesis of penicillin. as well as containing the gene encoding the second enzyme for penicillin biosynthesis, namely isopenicillin n synthetase (ipns) (designated ipna), our results show that these clones (psta200, psta201 and psta207) cont ... | 1990 | 2403928 |
| selective overexpression of the qute gene encoding catabolic 3-dehydroquinase in multicopy transformants of aspergillus nidulans. | the three enzymes necessary to catabolize quinate to protocatechuate are inducible by quinic acid, and transcription of their corresponding genes is controlled by the action of a positively acting activator gene and a negatively acting repressor gene. transformed strains of aspergillus nidulans containing multiple copies of the activator gene (quta) but single copies of the other qut genes retain normal regulation of the gene cluster and do not show any overexpression of the three quinic acid ca ... | 1990 | 2405841 |
| homologous transformation of cephalosporium acremonium with the nitrate reductase-encoding gene (niad). | we report the development of a homologous transformation system for cephalosporium acremonium using the niad gene of the nitrate assimilation (na) pathway. mutants in the na pathway were selected on the basis of chlorate resistance by conventional means. screening procedures were developed to differentiate between nitrate reductase apoprotein structural gene mutants (niad) and molybdenum cofactor gene mutants (cnx) as wt c. acremonium, unlike most filamentous fungi, fails to grow on minimal medi ... | 1990 | 2401400 |
| optimized vectors and selection for transformation of neurospora crassa and aspergillus nidulans to bleomycin and phleomycin resistance. | to provide a dominant selectable marker for transformation of neurospora crassa strains lacking specific auxotrophic mutations, we have engineered the bleomycin (bm) resistance-encoding gene (ble) from the bacterial transposon tn5 for expression in n. crassa. the coding region of the ble gene was fused to the promoter and terminator regions of the n. crassa am gene. in some vectors, multiple cloning sites were placed flanking the ble gene to provide a versatile ble cassette. when introduced into ... | 1990 | 1699844 |
| keratinophilic fungi and other moulds associated with air-dust particles from egypt. | one-hundred and eleven species and three species varieties belonging to 39 genera were collected from 50 dust samples on the five media used at 28 degrees c. using the hair-baiting technique with horse hair, 10 species of chrysosporium were isolated: c. asperatum, c. state of arthroderma tuberculatum, c. indicum, c. inops, c. keratinophilum, c. merdarium, c. pannorum, c. queenslandicum, c. tropicum and c. xerophilum. true dermatophytes were isolated: trichophyton verrucosum and trichophyton sp. ... | 1990 | 1702081 |
| characterization and comparison of mitochondrial dnas and rrnas from penicillium urticae and p. chrysogenum. | mitochondrial dna (mt dna) from a patulin producer, penicillium urticae (synonym p. griseofulvum), was 27.8 kb +/- 0.6 kb in size by electron microscopy and 27.2 kb by agarose gel electrophoresis. restriction endonuclease maps for nine restriction enzymes were constructed, and eleven fragments which covered the total range of the mt dna were cloned into the escherichia coli plasmid vector puc19. southern analysis of the native genomes of p. urticae and p. chrysogenum with six of the cloned fragm ... | 1990 | 1697325 |
| a negative regulator of mitosis in aspergillus is a putative membrane-spanning protein. | the temperature-sensitive cell cycle mutation bime7 of aspergillus nidulans causes cells to become blocked in mitosis at a restrictive temperature. previous work has shown that this mitotic block is induced even when cells are arrested in the s or g2 phase. the mitotic block is also observed in cells carrying a null mutation in bime, obtained by molecular disruption of the gene (osmani, s.a., engle, d.b., doonan, j.h., and morris, n.r. (1988) cell 52, 241-251), indicating that a lack of bime fun ... | 1990 | 1697851 |
| comparison and cross-species expression of the acetyl-coa synthetase genes of the ascomycete fungi, aspergillus nidulans and neurospora crassa. | the genes encoding the acetate-inducible enzyme acetyl-coenzyme a synthetase from neurospora crassa and aspergillus nidulans (acu-5 and faca, respectively) have been cloned and their sequences compared. the predicted amino acid sequence of the aspergillus enzyme has 670 amino acid residues and that of the neurospora enzyme either 626 or 606 residues, depending upon which of the two possible initiation codons is used. the amino acid sequences following the second alternative aug show 86% homology ... | 1990 | 1972535 |
| restriction enzyme analysis of mitochondrial dna of the aspergillus flavus group: a. flavus, a. parasiticus, and a. nomius. | mitochondrial dna restriction fragment length polymorphisms were identified that clearly distinguish aspergillus flavus, a. parasiticus, and a. nomius. mitochondrial dnas of a. flavus and a. parasiticus were found to be circular, and their size was estimated size to be 32 kilobases. a restriction map was constructed for the mitochondrial genome of an a. parasiticus isolate by using four restriction endonucleases. four genes tested were found to have the same order as in the mitochondrial genome ... | 1990 | 1976299 |
| use of nuclear dna restriction fragment length polymorphisms to analyze the diversity of the aspergillus flavus group: a. flavus, a. parasiticus, and a. nomius. | recombinant dna clones carrying high-copy or low-copy sequences from aspergillus nidulans and neurospora crassa were used to identify restriction fragment length polymorphisms (rflps) diagnostic for members of the a. flavus group: a. flavus, a. parasiticus, and a. nomius. these fungi were resolved into three distinct categories when they were grouped according to rflp patterns. subgroups within these categories were also evident. this limited rflp analysis of nuclear dna of members of the a. fla ... | 1990 | 1976300 |
| premeiotic disruption of the neurospora crassa malate synthase gene by native and divergent dnas. | repeat-induced point mutation (rip) has been used to generate new mutations in the previously uncharacterised gene for malate synthase in neurospora crassa. molecular clones carrying the am (nadp-glutamate dehydrogenase) gene and the malate synthase gene from either n. crassa or aspergillus nidulans have been introduced into neurospora as ectopic duplicate copies by transformation, selecting for the am+ function in a deletion host. a number of meiotic progeny derived from these transformants wer ... | 1990 | 1979142 |
| the regulatory gene area mediating nitrogen metabolite repression in aspergillus nidulans. mutations affecting specificity of gene activation alter a loop residue of a putative zinc finger. | the regulatory gene area mediating nitrogen metabolite repression in aspergillus nidulans has been sequenced and its transcript mapped and orientated. a single orf can encode a protein of 719 amino acids. a 52 amino acid region including a putative 'zinc finger' strongly resembles putative dna binding regions of the major regulatory protein of erythroid cells. the derived protein sequence also contains a highly acidic region possibly involved in gene activation and 22 copies of the motif s(t)pxx ... | 1990 | 1970293 |
| sequences of isopenicillin n synthetase genes suggest horizontal gene transfer from prokaryotes to eukaryotes. | evolutionary distances between bacterial and fungal isopenicillin n synthetase (ipns) genes have been compared to distances between the corresponding 5s rrna genes. the presence of sequences homologous to the ipns gene has been examined in dnas from representative prokaryotic organisms and ascomycotina. the results of both analyses strongly support two different events of horizontal transfer of the ipns gene from bacteria to filamentous fungi. this is the first example of such a type of transfer ... | 1990 | 1979440 |
| restriction fragment length polymorphisms in isolates of aspergillus fumigatus probed with part of the intergenic spacer region from the ribosomal rna gene complex of aspergillus nidulans. | differences in restriction fragment length polymorphisms (rflps) have been detected in isolates of aspergillus fumigatus. genomic dna from 11 isolates was digested with ecori, separated by electrophoresis, southern blotted and probed with dna from the intergenic spacer or non-transcribed spacer region of the rrna gene complex of aspergillus nidulans. three distinct rflp patterns were detected which differed from the control patterns observed with a. nidulans, aspergillus flavus and aspergillus n ... | 1990 | 1980128 |
| isolation and sequence analysis of a beta-tubulin gene from aspergillus flavus and its use as a selectable marker. | an altered beta-tubulin gene that confers resistance to benomyl [whose active ingredient is 2-(methoxycarbonylamino)benzimidazole (mbc)] was isolated from a dna library of aspergillus flavus and used as a selectable marker for transformation. the beta-tubulin gene was cloned into a plasmid vector containing the pyr-4 gene of neurospora crassa, and transformants were selected either for uracil prototrophy or mbc resistance. transformants selected for uracil prototrophy were of three phenotypic cl ... | 1990 | 2128007 |
| expression of the aspergillus niger glucose oxidase gene in a. niger, a. nidulans and saccharomyces cerevisiae. | we report the cloning of the aspergillus niger glucose oxidase gene and its use to elevate glucose oxidase productivity in a. niger by increasing the gene dosage. in addition, the gene has been introduced into a. nidulans where it provides the novel capacity to produce glucose oxidase. a plasmid, in which dna encoding the mature form of glucose oxidase was preceded by a saccharomyces cerevisiae secretion signal, effected high-level production of extracellular glucose oxidase in this yeast. | 1990 | 2076553 |
| genetic analysis of suppressors of the vea1 mutation in aspergillus nidulans. | light-dependent conidiation in the filamentous ascomycete, aspergillus nidulans, is contingent on the allelic state of the velvet (vea) gene. light dependence is abolished by a mutation in this gene (vea1), which allows conidiation to occur in the absence of light. we have isolated and characterized six extragenic suppressors of vea1 that restore the light-dependent conidiation phenotype. alleles of four genes, defined by complementation tests, were subjected to extensive genetic and phenotypic ... | 1990 | 2076818 |
| fungi of virgin and cultivated soil of salhiah desert, egypt. | 27 species and 13 genera of fungi were identified from virgin and cultivated soil of salhiah. the most abundant species of phosphate solubilizing fungi were aspergillus nidulans, a. niger, a flavus, penicillium lilacinum, p. frequentans and fusarium moniliforme. on cellulose agar the most prevalent species were chaetomium bostrychodes, c. olivaceum, humicola fuscoatra, aspergillus flavus, a. nidulans, a. niger, a. ochraceus, fusarium solani and f. oxysporum. on xylan agar aspergillus fumigatus, ... | 1990 | 2077791 |
| structure and molecular mechanics of ferrirhodin. | c41h64fen9o17.7 1/2h2o, mr = 1146.0, orthorhombic, p2(1)2(1)2(1), a = 9.740 (7), b = 16.764 (10), c = 32.632 (17) a, v = 5328 (6) a3, z = 4, d chi = 1.43 g cm-3, mo k alpha, lambda = 0.71069 a, mu = 3.26 cm-1, f(000) = 2428, t = 138 (2) k, r = 0.0986 for 3543 observed reflections. ferrirhodin, a ferrichrome siderophore (iron transport agent) was isolated from low-iron cultures of aspergillus versicolor and a. nidulans. the compound is isomeric with another microbial siderophore, ferrirubin, but ... | 1990 | 2088417 |
| beta-lactam antibiotic biosynthetic genes have been conserved in clusters in prokaryotes and eukaryotes. | a cosmid clone containing closely linked beta-lactam antibiotic biosynthetic genes was isolated from a gene library of flavobacterium sp. sc 12,154. the location within the cluster of the dna thought to contain the gene for delta-(l-alpha-aminoadipyl)-l-cysteinyl-d-valine synthetase (acvs), the first step in the beta-lactam antibiotic biosynthetic pathway, was identified by a novel method. this dna facilitated the isolation, by cross-hybridization, of the corresponding dna from streptomyces clav ... | 1990 | 2107074 |
| the beta-tubulin gene of epichloë typhina from perennial ryegrass (lolium perenne). | epichloë typhina is a biotrophic fungal pathogen which causes choke disease of pooid grasses. the anamorphic state, acremonium typhinum, is placed in the section albo-lanosa along with related, mutualistic, seed-disseminated endophytes. as an initial study of gene structure and evolution in epichloë and related endophytes, the beta-tubulin gene, tub2, of the perennial ryegrass choke pathogen (etprg) was cloned and sequenced. the coding sequence and the predicted beta-tubulin amino acid sequence ... | 1990 | 2147581 |
| a critical arginine in the large subunit of ribulose bisphosphate carboxylase/oxygenase identified by site-directed mutagenesis. | rapid inactivation by phenylglyoxal of ribulose bisphosphate carboxylase/oxygenase (ribulose-p2 carboxylase) from the cyanobacterium anacystis nidulans suggests the presence of an essential arginine, the modification of which is reduced in the presence of the substrate ribulose bisphosphate. arginine 292 in the large subunit of ribulose-p2 carboxylase from a. nidulans was chosen for site-directed mutagenesis studies on the basis of the complete conservation of this residue in corresponding seque ... | 1990 | 2108139 |
| induced expression of the aspergillus nidulans qute gene introduced by transformation into neurospora crassa. | the qa-2 gene of neurospora crassa encodes catabolic dehydroquinase which catabolizes dehydroquinic acid to dehydroshikimic acid. the qute gene of aspergillus nidulans corresponds to the qa-2 gene of n. crassa. the plasmid peh1 containing the qute gene from a. nidulans was used to transform a qa-2- strain of n. crassa. in southern blot analyses, dnas isolated from these transformants hybridized specifically to the qute gene probe. northern blot analyses indicated that qute mrna was produced in t ... | 1990 | 2148798 |
| of moulds and men, or two fingers are not better than one. | 1990 | 2149222 | |
| brla requires both zinc fingers to induce development. | expression of the aspergillus nidulans brla gene induces a developmental pathway leading to the production of asexual spores. we have introduced mutations into brla that are expected to disrupt either or both cys2-his2 zn(ii) coordination sites postulated to exist in the brla polypeptide. the resultant brla alleles fail to induce either the asexual reproductive pathway or the expression of development-specific genes. these data support the hypothesis that brla encodes a nucleic acid-binding prot ... | 1990 | 2108321 |
| acyl coenzyme a: 6-aminopenicillanic acid acyltransferase from penicillium chrysogenum and aspergillus nidulans. | a study of the final stages of the biosynthesis of the penicillins in penicillium chrysogenum has revealed two types of enzyme. one hydrolyses phenoxymethyl penicillin to 6-aminopenicillanic acid (6-apa). the other, also obtained from aspergillus nidulans, transfers a phenylacetyl group from phenylacetyl coa to 6-apa. the acyltransferase, purified to apparent homogeneity, had a molecular mass of 40 kda. it also catalyses the conversion of isopenicillin n (ipn) to benzylpenicillin (pen g) and hyd ... | 1990 | 2110531 |
| endo-exonuclease of aspergillus nidulans. | endo-exonuclease (ee) has been found in both active and inactive, but trypsin-activatable, forms in aspergillus nidulans. active ee was present mainly in nuclei, mitochondria, and vacuoles, while trypsin-activatable ee was mainly in the cytosol. the active form accounts for over 90% of the neutral deoxyribonuclease activity extracted from mycelia. a single strand (ss) dna-binding ee associated with a 28 kilodalton (kda) polypeptide was partially purified and characterized. it was found to closel ... | 1990 | 2161674 |
| regulation of inorganic sulfate activation in filamentous fungi. allosteric inhibition of atp sulfurylase by 3'-phosphoadenosine-5'-phosphosulfate. | atp sulfurylases from penicillium chrysogenum, penicillium duponti, aspergillus nidulans, and neurospora crassa are strongly inhibited by 3'-phosphoadenosine-5'-phosphosulfate (paps), the product of the second (adenosine-5'-phosphosulfate kinase-catalyzed) reaction in the two-step activation of inorganic sulfate. the v versus [paps] plots are sigmoidal. at physiological concentrations of mgatp (0.17-3 mm) and so4(2-) (0.4-10 mm), the [i]0.5 for paps inhibition of the p. chrysogenum enzyme is 35- ... | 1990 | 2162344 |
| cloning and expression of a hybrid streptomyces clavuligerus cefe gene in penicillium chrysogenum. | a hybrid cefe gene was constructed by juxtaposing promoter sequences from the penicillium chrysogenum pcbc gene to the open reading frame of the streptomyces clavuligerus cefe gene. in s. clavuligerus the cefe gene codes for the enzyme penicillin n expandase [also known as deacetoxycephalosporin c synthetase (daocs)]. to insert the hybrid cefe gene into p. chrysogenum the vector pps65 was constructed; pps65 contains the hybrid cefe gene and the aspergillus nidulans amds gene. the amds gene encod ... | 1990 | 2111228 |
| characterization of cryptic plasmids from marine cyanobacteria and construction of a hybrid plasmid potentially capable of transformation of marine cyanobacterium, synechococcus sp., and its transformation. | among forty strains of marine cyanobacteria isolated in our laboratory, five strains had 1-3 different plasmids. the unicellular marine cyanobacterium, synechococcus sp. nkbg 042902, contains at least three plasmids (psy09, psy10, and psy11). however, these plasmids are cryptic. therefore, a hybrid plasmid pusy02 containing the 1.4-kb hindiii fragment of psy11 and escherichia coli plasmid puc18 was constructed. the plasmid pusy02 transformed both marine synechococcus sp. nkbg042902-yg1116, which ... | 1990 | 2112896 |
| [lung mycoses caused by opportunistic fungi]. | 1990 | 2128860 | |
| mutation of a gene that encodes a kinesin-like protein blocks nuclear division in a. nidulans. | in a. nidulans, the temperature-sensitive cell cycle mutation bimc4 causes an elevated mitotic index at restrictive temperature. under restrictive conditions the mutation interferes with separation of the spindle pole bodies, causes abnormal spindle morphology, and prevents nuclear division. we have cloned and sequenced the wild-type bimc gene. the predicted protein product has homology to drosophila kinesin heavy chain. we conclude that this kinesin-like protein has an important role in nuclear ... | 1990 | 2138511 |
| adenosine triphosphatases in p-fluorophenylalanine sensitive and resistant strains of aspergillus nidulans. | 1990 | 2144842 | |
| immunoprecipitation distinguishes non-overlapping groups of snrnps in schizosaccharomyces pombe. | the large number of snrnas in the fission yeast schizosaccharomyces pombe can be divided into four non-overlapping groups by immunoprecipitation with antibodies directed against mammalian snrnp proteins. 1) of the abundant snrnas, anti-sm sera precipitate only the spliceosomal snrnas u1, u2, u4, u5 and u6. surprisingly, three sm-sera tested distinguish between u2, u4 and u5 and u1 from s.pombe; one precipitating only u1 and two precipitating u2, u4 and u5 but not u1. 2) a group of 11 moderately ... | 1990 | 2144896 |
| nucleotide sequences of the meta-cleavage pathway enzymes 2-hydroxymuconic semialdehyde dehydrogenase and 2-hydroxymuconic semialdehyde hydrolase from pseudomonas cf600. | the nucleotide sequence of a 2493 base pair (bp) region, spanning the coding regions for the meta-cleavage pathway enzymes 2-hydroxymuconic semialdehyde dehydrogenase (hmsd) and 2-hydroxymuconic semialdehyde hydrolase (hmsh), was determined. the deduced protein sequence for hmsd is 486 amino acid residues long with an mr of 51,682. hmsd has homology with a number of aldehyde dehydrogenases from various eukaryotic sources. the deduced protein sequence for hmsh is 283 amino acids long with an mr o ... | 1990 | 2194577 |
| novel potential mitotic motor protein encoded by the fission yeast cut7+ gene. | the structure equivalent to higher eukaryotic centrosomes in fission yeast, the nuclear membrane-bound spindle pole body, is inactive during interphase. on transition from g2 to m phase of the cell cycle, the spindle pole body duplicates; the daughter pole bodies seed microtubules which interdigitate to form a short spindle that elongates to span the nucleus at metaphase. we have identified two loci which, when mutated, block spindle formation. the predicted product of one of these genes, cut7+, ... | 1990 | 2145514 |
| [host-vector systems in filamentous fungi]. | 1990 | 2148397 | |
| cloned manganese superoxide dismutase reduces oxidative stress in escherichia coli and anacystis nidulans. | the mn superoxide dismutase gene of escherichia coli was subcloned into the e. coli-anacystis nidulans shuttle vector psg111 to make the plasmid pmyg1. transformation of e. coli hb101 with pmyg1 resulted in a 6-fold increase in superoxide dismutase activity. there was also induction of mn superoxide dismutase in the transformants upon exposure to paraquat, as evidenced by dramatically increased levels of the mn superoxide dismutase polypeptide in cytoplasmic extracts and a 16-fold further increa ... | 1990 | 2157207 |
| cloning, characterization of the acyl-coa:6-amino penicillanic acid acyltransferase gene of aspergillus nidulans and linkage to the isopenicillin n synthase gene. | the pende gene encoding acyl-coa:6-amino penicillanic acid acyltransferase (aat), the last enzyme of the penicillin biosynthetic pathway, has been cloned from the dna of aspergillus nidulans. the gene contains three introns which are located in the 5' region of the open reading frame. it encodes a protein of 357 amino acids with a molecular weight of 39,240 da. the pende gene of a. nidulans shows 73% similarity at the nucleotide level with the pende gene of penicillium chrysogenum. the a. nidula ... | 1990 | 2166227 |
| efficient integrative transformation of the phytopathogenic fungus alternaria alternata mediated by the repetitive rdna sequences. | an attempt was made to transform alternaria alternata protoplasts using a plasmid vector, pdh25, bearing the escherichia coli hygromycin b (hy) phosphotransferase gene (hph) under the control of the aspergillus nidulans trpc promoter. transformants arose on a selective medium containing 100 micrograms hy/ml. there were two types of transformants, forming large and small colonies on the selective medium. transformation with one microgram of the vector produced an average of 4.5 large colonies and ... | 1990 | 2169442 |
| insertional inactivation and cloning of the wa gene of aspergillus nidulans. | we describe examples of wa gene inactivation (resulting in white conidiospores) obtained during transformation of aspergillus nidulans. one wa- transformant was obtained by transformation with a prn+ plasmid of a strain with green conidia (wa+) which was unable to catabolize l-proline (prn-). this transformant contains a very large number of plasmid copies integrated at a single site inseparable from the wa locus. passage of this transformant through the sexual cycle generated a variety of novel ... | 1990 | 2172077 |
| the upstream region of the ipns gene determines expression during secondary metabolism in aspergillus nidulans. | we have constructed a translational fusion between the isopenicillin-n-synthetase-encoding gene (ipns) of aspergillus nidulans and the lacz gene of escherichia coli. recombinant strains carrying a single copy of the fusion integrated at the ipns locus produced beta-galactosidase (beta gal) during secondary metabolism. integration of the fusion at the argb locus results in a situation in which the only 5'-flanking sequences of the ipns gene upstream from the chimeric fused gene are those included ... | 1990 | 2115487 |
| upstream elements repress premature expression of an aspergillus developmental regulatory gene. | the aspergillus nidulans abaa gene regulates intermediate steps in asexual reproductive development and is itself developmentally regulated. an 822-base-pair dna fragment from the abaa 5'-flanking region is sufficient to drive developmentally appropriate expression of the escherichia coli lacz gene. deletion analysis showed that this fragment contains elements that repress transcription in vegetative cells and immature conidiophores and that activate transcription later during development. a 45- ... | 1990 | 2117702 |
| an inversion truncating the crea gene of aspergillus nidulans results in carbon catabolite derepression. | the cread-30 mutation leading to carbon catabolite derepression in aspergillus nidulans is a pericentric inversion, having one breakpoint within the crea gene on the left arm of chromosome i and the other breakpoint between bing and ya on the right arm. the left-arm breakpoint alters the crea transcript. the likelihood that the inversion truncates crea centrally strengthens a previous proposal that derepression is the phenotype of loss-of-function mutations in crea. | 1990 | 2201871 |
| transformation of aspergillus giganteus to hygromycin b resistance. | a wild strain of a. giganteus was transformed to hygromycin b resistance using a bacterial resistance gene under the control of a. nidulans sequences. stable transformants arose by heterogenous integration, mainly of tandem repeats of vector dna at various sites in the host genome. between 6 and 30 resistant colonies were obtained per microgram dna per 3 x 10(3) viable protoplasts. vector dna could be recovered by transformation of escherichia coli with undigested genomic dna from aspergillus gi ... | 1990 | 2178785 |
| chromosomal mapping of an alcc disruption with respect to amda in aspergillus nidulans. | we report the use of the ribob gene for a gene replacement in the alcc gene of aspergillus nidulans, and show by "reverse genetics" that the alcc gene is very closely linked to the amda gene. | 1990 | 2178787 |
| functional elements in the promoter region of the aspergillus nidulans gpda gene encoding glyceraldehyde-3-phosphate dehydrogenase. | analysis of the promoter region of the highly expressed aspergillus nidulans gpda gene is described. the nucleotide (nt) sequence of a 1.3-kb region upstream from the atg was determined. comparison with promoter regions of other aspergillus and neurospora genes revealed several regions of similar sequence. both random and site-specific mutations were introduced into the promoter region of the gpda gene, and the resulting mutant promoters were fused to the escherichia coli lacz gene. the construc ... | 1990 | 2121607 |
| purification and characterization of cellulolytic enzymes produced by aspergillus nidulans. | three exo-glucanases, two endo-glucanases and two beta-glucosidases were separated and purified from the culture medium of aspergillus nidulans. the optimal assay conditions for all forms of cellulase components ranged from ph 5.0 to 6.0 and 50 degrees c and 65 degrees c for exo-glucanases and endo-glucanases but 35 degrees c and 65 degrees c for beta-glucosidases. a close relation of enzyme stability to their optimal ph range was observed. all the cellulase components were stable for 10 min at ... | 1990 | 2179198 |
| molecular characterization of the acyl-coenzyme a:isopenicillin n acyltransferase gene (pende) from penicillium chrysogenum and aspergillus nidulans and activity of recombinant enzyme in escherichia coli. | the final step in the biosynthesis of beta-lactam antibiotics in penicillium chrysogenum and aspergillus nidulans involves removal of the l-alpha-aminoadipyl side chain from isopenicillin n (ipn) and exchange with a nonpolar side chain. the enzyme catalyzing this reaction, acyl-coenzyme a:isopenicillin n acyltransferase (acyltransferase), was purified from p. chrysogenum and a. nidulans. based on nh2-terminal amino acid sequence information, the acyltransferase gene (pende) from p. chrysogenum a ... | 1990 | 2120195 |
| isolation and molecular characterization of the aspergillus nidulans wa gene. | the walls of aspergillus nidulans conidia contain a green pigment that protects the spores from damage by ultraviolet light. at least two genes, wa and ya, are required for pigment synthesis: ya mutants produce yellow spores, wa mutants produce white spores, and wa mutations are epistatic to ya mutations. we cloned wa by genetic complementation of the wa3 mutation with a cosmid library containing nuclear dna inserts from the wild-type strain. the wa locus was mapped to an 8.5-10.5-kilobase regio ... | 1990 | 2227390 |
| qualitative and quantitative changes in beta-1,4-glucosidase accompanying growth of aspergillus nidulans. | a comparative study of the wild type a. nidulans and a mutant strain aco-t69 (lacking conidia and cleistothecia) revealed the better production of beta-1,4-glucosidase in the former. the relative distribution of the enzyme levels in various morphological structures viz. somatic hyphae, spores and cleistothecia also showed a variation. highest specific activity was found in the cleistothecial extracts. the electrophoretic analysis of the wild type strain demonstrated the presence of three isoenzy ... | 1990 | 2120420 |
| sequence and molecular structure of the aspergillus nidulans ya (laccase i) gene. | 1990 | 2192364 | |
| nucleotide and amino acid sequences of the conidium-specific spoc1-c1d gene from aspergillus nidulans. | 1990 | 2243803 | |
| a mutation which modifies the activity of a translational suppressor in aspergillus nidulans. | a third type of translational fidelity mutation has been induced in aspergillus nidulans. the new mutation enhances growth, in suppressing conditions, of a strain containing suppressor suac109 and antisuppressor asud14 and is called alob8 for its allosuppressor activity. compared with the progenitor strain (asud14, suac109), ribosomes from the new mutant (alob8, asud14, suac109) increase misincorporation of leucine in a poly(u)-dependent homologous cell-free assay. the misreading level is mainta ... | 1990 | 2207157 |
| genotoxicity studies on the organophosphorus insecticide chloracetophone. | chloracetophone (o,o-dimethyl-2,2,2-trichloro-1-(chloroacetoxy)phosphonate), a new insecticide of the organophosphorus group of pesticides, was tested for genotoxicity in a variety of systems with different genetic end-points and varying parameters. the test systems included 2 microbial systems, salmonella and aspergillus for point mutations and mitotic segregation, respectively, and human lymphocyte cultures and mammalian bone marrow cells (from rats and hamsters treated acutely and subacutely) ... | 1990 | 2179715 |
| conidium differentiation in aspergillus nidulans wild-type and wet-white (weta) mutant strains. | conidium (asexual spore) differentiation in wild-type and the wet-white (weta) mutant of aspergillus nidulans was compared in intact chains of successively older conidia. carbohydrate cytochemistry helped define three stages (stages i, ii, and iii) of wild-type conidium maturation on the basis of changes in the ultrastructure and composition of the conidium wall. conidia of the weta6 mutant strain formed normally but failed to mature during stages ii and iii. specifically, the inner wall layer o ... | 1990 | 2180753 |
| characterization of a glycerol kinase mutant of aspergillus niger. | a glycerol-kinase-deficient mutant of aspergillus niger was isolated. genetic analysis revealed that the mutation is located on linkage group vi. the phenotype of this mutant differed from that of a glycerol kinase mutant of aspergillus nidulans in its ability to utilize dihydroxyacetone (dha). the weak growth on glycerol of the a. niger glycerol kinase mutant showed that glycerol phosphorylation is an important step in glycerol catabolism. the mutant could still grow normally on dha because of ... | 1990 | 2230717 |
| screening method for large numbers of dye-adsorbents for enzyme purification. | a method is described by means of which 96 different dye-adsorbents can be tested simultaneously for their ability to bind enzymes and to test their biospecific elution. small amounts of cell-free extract are applied to dye-adsorbents which are packed in a 96-well transplate cartridge. after biospecific elution, the amount of the eluted enzyme is tested in a microtitre plate assay. the method is illustrated by the purification of glycerol dehydrogenase (e.c. 1.1.1.72), 6-phosphogluconate dehydro ... | 1990 | 2205614 |
| a mutation affecting amds expression in aspergillus nidulans contains a triplication of a cis-acting regulatory sequence. | in aspergillus nidulans expression of the acetamidase structural gene, amds, is under the control of at least four regulatory genes including the trans-acting amda regulatory gene. a cis-acting mutation (amdi66) consisting of an 18 bp duplication in the 5' region of the amds gene results in very high levels of acetamidase activity but only in strains carrying semi-dominant mutations in the amda gene. in selecting for increased amds expression in an amdi66 and a+ strain, an a. nidulans strain wit ... | 1990 | 2187153 |
| chloroacetaldehyde is a powerful inducer of mitotic aneuploidy in aspergillus nidulans. | the vinyl chloride metabolite chloroacetaldehyde (caa) was tested for the induction of mitotic chromosome malsegregation in aspergillus nidulans. exposure of germinating conidia to caa (16-64 microm) produced high rates of abnormal colonies with segregation of the whole first chromosome in the diploid strain p1, and abnormal, putative hyperploids in the haploid strain 35, indicating that caa primarily induces abnormal chromosome segregation. comparative assays with the known spindle poison chlor ... | 1990 | 2188069 |
| isozyme polymorphism of endo-beta-1,4-glucanase in aspergillus nidulans. | an electrophoretic survey of the natural populations of aspergillus nidulans, the a. nidulans group, and various species belonging to the genus aspergillus from diverse geographical areas of india was carried out to determine the isozyme polymorphism of endoglucanase. the data revealed the presence of three forms of endoglucanase designated eg i, eg ii, and eg iii. in some isolates, eg i and eg ii were present separately; in others, instead of two separate bands, one thick band was detected, whi ... | 1990 | 2188645 |
| gamma-tubulin is a component of the spindle pole body that is essential for microtubule function in aspergillus nidulans. | we have recently discovered that the mipa gene of a. nidulans encodes gamma-tubulin, a new member of the tubulin superfamily. to determine the function of gamma-tubulin in vivo, we have created a mutation in the mipa gene by integrative transformation, maintained the mutation in a heterokaryon, and determined the phenotype of the mutation in spores produced by the heterokaryon. the mutation is lethal and recessive. it strongly inhibits nuclear division, less strongly inhibits nuclear migration, ... | 1990 | 2194669 |
| abaa controls phialide differentiation in aspergillus nidulans. | aspergillus nidulans is an ascomycetous fungus that reproduces asexually by forming multicellular conidiophores and uninucleate spores called conidia. loss of function mutations in the abacus a (abaa) regulatory locus result in formation of aberrant conidiophores that fail to produce conidia. wild-type conidiophores form two tiers of sterigmata. the first tier, metulae, divide to produce the second tier, phialides. phialides are sporogenous cells that produce conidia through a specialized apical ... | 1990 | 2152124 |
| frequency of spontaneous and induced recessive mutations in a diploid strain of aspergillus nidulans. | the spontaneous and uv-induced frequencies of recessive mutations have been studied in a diploid strain of aspergillus nidulans, by the p-fluoro-phenylalanine (fpa) and 8-azaguanine (8-aza) resistance tests, on either resting or germinating conidia. observed frequencies are in the order of magnitude of those expected, which have been calculated considering the observed mutation frequencies in the haploid strain as well as the mitotic recombination frequencies. we also review some papers which cl ... | 1990 | 2197553 |
| cloning of the dna repair gene, uvsf, by transformation of aspergillus nidulans. | as a first step in the cloning of the dna repair gene uvsf of aspergillus nidulans, uvsf pyrg double mutant strains were transformed with a genomic library which carried the complementing neurospora pyr-4 gene in the vector. rare pyr+ uvs+ cotransformants were obtained on media lacking pyrimidines, overlayed with mms (methyl-methane sulfonate) to which uvsf is hypersensitive. among mms-resistant transformants, southerns revealed two types which showed single bands of different sizes when bglii-d ... | 1990 | 2199316 |
| sequence, organization and expression of the core histone genes of aspergillus nidulans. | the core histone gene family of aspergillus nidulans was characterized. the h2a, h2b and h3 genes are unique in the a. nidulans genome. in contrast there are two h4 genes, h4.1 and h4.2. as previously reported for the h2a gene (may and morris 1987) introns also interrupt the other core histone genes. the h2b gene, like the h2a gene, is interrupted by three introns, the h3 and h4.1 gene are each interrupted by two introns and the h4.2 gene contains one intron. the position of the single intron in ... | 1990 | 2274040 |
| characterization and expression of the unique calmodulin gene of aspergillus nidulans. | complete cdna and genomic clones for the unique calmodulin (cam) gene of the filamentous fungus aspergillus nidulans have been isolated and characterized. the gene contains five introns, of which three are at unique positions relative to other cam genes. the a. nidulans cam gene is transcribed as a single, 0.85-kilobase mrna species that encodes a predicted protein 84% identical (93% similar if conservative changes are considered) to vertebrate cam. the complete cdna was ligated into a lambda pl ... | 1990 | 2199442 |
| the molecular cloning and identification of a gene product specifically required for nuclear movement in aspergillus nidulans. | a temperature-sensitive mutation in the nudc gene (nudc3) of aspergillus nidulans specifically prevents the microtubule-based movement of nuclei in this organism at the restrictive temperature. the mutation does not affect short term growth, nuclear division, or the movement of other subcellular organelles. immunofluorescence analysis of cells blocked at the restrictive temperature, using antitubulin antibodies, shows that the inability of nuclei to move under these conditions is not related to ... | 1990 | 2199460 |