Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| brucella requires a functional type iv secretion system to elicit innate immune responses in mice. | the virb operon, encoding a type iv secretion system (t4ss), is essential for intracellular survival and persistent infection by brucella spp. to better understand the role of the t4ss in evading host defence mechanisms and establishing chronic infection, we compared transcriptional profiles of the host response to infection with wild-type and virb mutant brucella strains. analysis of gene expression profiles in murine splenocytes 3 days after inoculation with wild-type brucella strains revealed ... | 2007 | 17441987 |
| brucellosis as a cause of acute febrile illness in egypt. | to develop better estimates of brucellosis incidence, we conducted population-based surveillance for acute febrile illness (afi) in fayoum governorate (population 2347249), egypt during two summer periods (2002 and 2003). all hospitals and a representative sample of community healthcare providers were included. afi patients without obvious etiology were tested for brucellosis by culture and serology. incidence estimates were calculated adjusting for sampling methodology and study period. of 4490 ... | 2007 | 17442354 |
| a recombinant subunit vaccine based on the insertion of 27 amino acids from omp31 to the n-terminus of bls induced a similar degree of protection against b. ovis than rev.1 vaccination. | the development of an effective subunit vaccine against brucellosis is a research area of intense interest. the enzyme lumazine synthase from brucella spp. (bls) is highly immunogenic, presumably due to its decameric arrangement and remarkable stability. in this work we decided to develop a chimera with the scaffold protein bls decorated with 10 copies of a known protective epitope derived from an outer membrane protein of 31kda (omp31) from brucella spp. vaccination of balb/c mice with the chim ... | 2007 | 17442465 |
| tigecycline: its potential for treatment of brucellosis. | the in vitro efficacy and synergistic activity of tigecycline in comparison with other antimicrobials used in brucellosis, were tested for 16 brucella melitensis strains by the e-test method. tigecycline had the lowest minimal inhibitory concentration levels, and rifampin the highest, in the study. tigecycline also provided the better synergistic activity compared to doxycycline according to the fractional inhibitory concentration index. the results of this in vitro study suggest tigecycline as ... | 2007 | 17464866 |
| unusual causes of intrahepatic cholestatic liver disease. | we report five cases with unusual causes of intrahepatic cholestasis, including consumption of teucrium polium (family lamiaceae) in the form of tea, stauffer's syndrome, treatment with tamoxifen citrate for breast cancer, infection with coxiella burnetii (acute q fever), and infection with brucella melitensis (acute brucellosis). | 2007 | 17465487 |
| the liver in brucellosis. | brucellosis involves the liver in varying ways, ranging from benign subclinical increases in serum aminotransferase levels to ominous chronic suppurative disease. data on histopathology of the liver in brucellosis are scarce and contradictory. we sought to determine the liver histologic patterns present in a series of brucellosis patients and review the existing knowledge about liver involvement in this worldwide, prevalent zoonotic infection. | 2007 | 17482524 |
| prosthetic hip infection due to brucella melitensis: case report and literature review. | prosthetic joint infection (pji) due to brucella spp. is extremely rare. we report the case of a prosthetic hip infection due to brucella melitensis in a 51-year-old male patient. the initial presentation was a gluteal abscess. there was radiographic evidence of implant loosening. the patient was cured after prolonged treatment with streptomycin, rifampicin, and doxycycline, followed by 2-stage exchange of the prosthesis. brucella spp. should be considered in the differential diagnosis of pji in ... | 2007 | 17509788 |
| [experimental evaluation of the brucellosis therapeutic vaccine efficacy]. | use of antibiotics can't completely solve the problem of brucellosis treatment, especially its chronic forms, because antibacterial preparations do not eliminate main pathogenetic factor of the disease--sensibilization of the macroorganism. it makes actual the question about complex immuno- and antibacterial therapy. long-term clinical experience proved high effectiveness of a therapeutic brucellosis vaccine. earlier this preparation was manufactured in research institute of vaccines and sera in ... | 2007 | 17523477 |
| development and evaluation of real-time polymerase chain reaction assays on whole blood and paraffin-embedded tissues for rapid diagnosis of human brucellosis. | this study aimed at developing a real-time polymerase chain reaction (pcr) assay for the rapid diagnosis of human brucellosis on clinical specimens. three assays with hybridization probe detection on the lightcycler instrument were developed and compared targeting the 16s-23s internal transcribed spacer region (its) and the genes encoding for omp25 and omp31. all assays showed 100% analytical sensitivity and 100% specificity when tested on 28 consecutive clinical isolates of brucella sp. and 19 ... | 2007 | 17532591 |
| brucella melitensis infection of a branchial cyst. | 2007 | 17548002 | |
| mutations of the quorum sensing-dependent regulator vjbr lead to drastic surface modifications in brucella melitensis. | successful establishment of infection by bacterial pathogens requires fine-tuning of virulence-related genes. quorum sensing (qs) is a global regulation process based on the synthesis of, detection of, and response to small diffusible molecules, called n-acyl-homoserine lactones (ahl), in gram-negative bacteria. in numerous species, qs has been shown to regulate genes involved in the establishment of pathogenic interactions with the host. brucella melitensis produces n-dodecanoyl homoserine lact ... | 2007 | 17557825 |
| poly(d,l-lactide-coglycolide) particles containing gentamicin: pharmacokinetics and pharmacodynamics in brucella melitensis-infected mice. | drug delivery systems containing gentamicin were studied as a treatment against experimental brucellosis in mice. micro- and nanoparticles prepared by using poly(d,l-lactide-coglycolide) (plga) 502h and microparticles made of plga 75:25h were successfully delivered to the liver and the spleen, the target organs for brucella melitensis. both polymers have the same molecular weight but have different lactic acid/glycolic acid ratios. microparticles of plga 502h and 75:25h released their contents i ... | 2007 | 17220415 |
| nasal immunization with recombinant brucella melitensis bp26 and trigger factor with cholera toxin reduces b. melitensis colonization. | bp26 and trigger factor (tf) dna vaccines have previously been shown to protect against brucella infection. in this study, purified bp26 and tf proteins were tested in balb/c mice for immunity and protection. the results showed that intranasal (i.n.) immunization with bp26 and tf in conjunction with cholera toxin (ct) adjuvant elicit both elevated mucosal and systemic immune responses. while nasal immunization with either bp26 or tf elicited elevated antibody responses, co-immunization with both ... | 2007 | 17239499 |
| the use of elisa in a seroprevalence study of brucella antibodies in west bank palestinian women of childbearing age. | 2007 | 17241807 | |
| implantable cardioverter-defibrillator infection due to brucella melitensis: case report and review of brucellosis of cardiac devices. | we report a case of implantable cardioverter-defibrillator infection due to brucella melitensis and review 5 previously reported cases of brucella infection of cardiac devices. device removal, followed by antibiotic therapy for 6 weeks, is probably required for cure. although rare, reports of brucella infection of prosthetic implants and devices have increased in the past decade. brucellosis should be considered in the differential diagnosis of cardiac device infection in patients residing in or ... | 2007 | 17243046 |
| pcr confirmation of cutaneous manifestation due to brucella melitensis. | human brucellosis, a zoonotic disease, is endemic in the belgaum district, karnataka, india. a male patient presented with a generalized itchy rash. blood was sent for venereal disease research laboratory testing. screening was carried out for brucella antibodies following hospital policy and diagnosis was confirmed by pcr. | 2007 | 17244815 |
| medically treated paravertebral brucella abscess presenting with acute torticollis: case report. | atlantoaxial subluxation secondary to a paravertebral (retropharyngeal) abscess is a well known but rare event in children. | 2007 | 17254895 |
| validation of a fluorescence polarization assay (fpa) performed in microplates and comparison with other tests used for diagnosing b. melitensis infection in sheep and goats. | fluorescence polarization assay (fpa) is a relatively new test for the serological diagnosis of brucella spp. infection in animals. fpa, carried out in 96-well microplate format, was validated here for diagnosing b. melitensis infection in sheep and goats. this study included sera from 1933 sheep and goats, from animals reared in naturally infected flocks (verified by culture) and showing a positive reaction to two different tests conducted in parallel. in addition, 2154 sera originating from he ... | 2007 | 17258229 |
| application of the fluorescence polarization assay for detection of caprine antibodies to brucella melitensis in areas of high prevalence and widespread vaccination. | the screening rose bengal test (rbt), the buffered plate agglutination test (bpat), and the confirmatory complement fixation test (cft) are currently approved by the world organization for animal health (oie) for diagnosis of goat brucellosis. however, rbt (at 3% or 8% cell concentration) is known to be affected by vaccinal antibodies. in the present study, mexican and canadian oie tests were compared with the fluorescence polarization assay (fpa), alone or in combination, using indirect and com ... | 2007 | 17267588 |
| cell-mediated immune responses differentiate infections with brucella suis from yersinia enterocolitica serotype o:9 in pigs. | due to almost identical lipopolysaccharide (lps) o-antigens, infections with yersinia enterocolitica serotype o:9 (yeo:9) cause false positive serological reactions (fpsr) in tests for brucella and thus cause problems in national brucella surveillance programs. as lps are strong inducers of antibody responses it was hypothesized that cell-mediated immune responses to non-lps antigens of the two bacteria can be used to separate immune responses to these two biologically very different infections. ... | 2007 | 17270281 |
| prepatellar brucella melitensis bursitis: case report and literature review. | bursal infection is an uncommon clinical presentation of brucellosis. we herein report a case of brucella melitensis bursitis that was successfully treated. other reported cases of brucella bursitis were reviewed. a high degree of suspicion in the right clinical setting is required for the diagnosis of brucella bursitis. | 2007 | 17308857 |
| suitability of partial 16s ribosomal rna gene sequence analysis for the identification of dangerous bacterial pathogens. | in a bioterrorism event a rapid tool is needed to identify relevant dangerous bacteria. the aim of the study was to assess the usefulness of partial 16s rrna gene sequence analysis and the suitability of diverse databases for identifying dangerous bacterial pathogens. | 2007 | 17309636 |
| dissecting aneurysm of the thoracic aorta in a patient with nephrotic syndrome and brucellosis. | we report the case of a 61-year-old man with nephrotic syndrome due to glomerulonephritis and chronic brucellosis complicated by dissecting aortic aneurysm. the patient worked as a veterinarian and was diagnosed for chronic but non-active brucellosis with positive serum test for brucella melitensis in the past. administration of cyclosporine in combination with low dose prednisone resulted at least in proteinuria reduction and partial remission for 3 years. dissecting aortic aneurysm was treated ... | 2007 | 17318348 |
| irreversible papillitis and ophthalmoparesis as a presenting manifestation of neurobrucellosis. | a 35-year-old man presented with a meningeal syndrome and acute onset of visual blurring. clinical investigations revealed bacterial meningitis with bilateral papillitis and ophthalmoparesis. serum and cerebrospinal fluid serology confirmed the diagnosis of chronic active neurobrucellosis. following therapy there was no improvement and he developed optic atrophy. extensive literature review revealed, one case of bilateral irreversible papillitis resulting from neurobrucellosis. however no cases ... | 2007 | 17320277 |
| diagnostic yield of blood clot culture in the accurate diagnosis of enteric fever and human brucellosis. | culture of blood is the most frequent, accurate means of diagnosing bacteremia in enteric fever and brucellosis. however, conventional blood culturing is slow in isolating bacteria causing these diseases. in this work, we evaluated the performance of blood clot culture and conventional whole blood cultures in the accurate diagnosis of enteric fever (253 cases) and human brucellosis (71cases). the blood clot culture was found to be much more sensitive for both salmonella (more by 34.4%, p< 0.001) ... | 2007 | 17323826 |
| brucella melitensis in the aetiology of febrile neutropenia: report of two cases brucellosis and febrile neutropenia. | 2007 | 17343671 | |
| asymptomatic brucella bacteraemia and isolation of brucella melitensis biovar 3 from human breast milk. | brucellosis is a zoonotic disease and virtually all infections derived from exposure to animals or ingestion of unpasteurized dairy products. brucellosis among family members has been reported. however, screening household members of an index case of acute brucellosis is not a routine procedure. a 10-y-old boy was diagnosed with acute brucellosis. unpasteurized goat cheese commonly consumed within the family was thought to be the possible source of the bacteria. the family (parents, sister and b ... | 2007 | 17366048 |
| the lov domain: a chromophore module servicing multiple photoreceptors. | three different families of blue-light receptors have been characterized from higher plants: three cryptochromes, two phototropins, and the three members of the ztl/ado family. phototropins and the ztl/ado proteins have chromophore modules, designated lov domains, that bind flavin mononucleotide and undergo formation of a c(4a) flavin-cysteinyl adduct. all contain the highly conserved amino acid motif gxncrflq. over 90 prokaryote proteins also contain lov domains with this motif upstream from on ... | 2007 | 17380429 |
| polymorphism of the transmembrane region of the mica gene and human brucellosis. | we investigated the polymorphism of the transmembrane region of the mica gene (major histocompatibility complex class i chain-related gene a) in relation to susceptibility to human brucellosis. we typed 114 patients with brucellosis and 121 healthy controls for mica transmembrane polymorphism with polymerase chain reaction methods combined with fluorescent technology. we found a significant decrease in the frequency of the mica-a4 allele in the patients with brucellosis compared with the control ... | 2007 | 17389022 |
| congenital brucellosis: a rare cause of respiratory distress in neonates. | brucellosis represents a rare cause of neonatal infection. in this article we report a very unusual case of congenital infection due to brucella melitensis in a term neonate presenting after birth with severe respiratory distress and radiological manifestations (lobar consolidation and diffuse interstitial infiltrations) compatible with pulmonary involvement. the neonate was successfully treated with trimethoprim-sulfamethoxazole, rifampicin, and gentamicin. | 2007 | 17597440 |
| a dna vaccine coding for the chimera blsomp31 induced a better degree of protection against b. ovis and a similar degree of protection against b. melitensis than rev.1 vaccination. | in the present study, we reported an attempt to improve the immunogenicity and protective capacity of the chimera blsomp31 using a different antigen delivery: dna vaccination. vaccination of balb/c mice with the dna vaccine coding for the chimera blsomp31 (pciblsomp31) provided the best protection level against brucella ovis, which was significantly higher than the given by the co-delivery of both plasmids coding for the whole proteins (pcdnabls+pciomp31) and even higher than the control vaccine ... | 2007 | 17600596 |
| seroprevalence of and risk factors for brucellosis of goats in herds of michoacan, mexico. | our objectives were to estimate the seroprevalence of brucella melitensis, and to identify some risk factors associated with goat seropositivity in michoacan, mexico. blood samples were collected from 5114 animals from 79 herds. sera were tested for antibodies against b. melitensis using the rose bengal plate test and the complement-fixation test. information regarding the herds and each animal sampled were recorded through a personal interview at the farm. we used random-effects multivariable l ... | 2007 | 17604858 |
| actual antibiotic resistance pattern of brucella melitensis in central anatolia. an update from an endemic region. | to test in vitro susceptibilities of brucella melitensis (b. melitensis) blood isolates obtained from an endemic region, by broth microdilution susceptibility test. | 2007 | 17676210 |
| characterisation of human outbreaks of brucellosis and sporadic cases by the use of hyper-variable octameric oligonucleotide fingerprint (hoof) variable number tandem repeats. | hyper-variable octameric oligonucleotide fingerprints (hoofs) enable typing of brucella spp. by targeting the 8-bp tandem repeat in eight loci that vary in number (variable number tandem repeats; vntrs). brucella is one of the most important zoonotic pathogens, because of its public health and economic consequences. to assess the role of hoofs as epidemiological markers for brucella melitensis, which is the main species involved in human brucellosis in spain, 87 sporadic and outbreak isolates we ... | 2007 | 17686139 |
| partial protection against brucella infection in mice by immunization with nonpathogenic alphaproteobacteria. | previous findings indicate that brucella antigens and those from nonpathogenic alphaproteobacteria (npap) are cross-recognized by the immune system. we hypothesized that immunization with npap would protect mice from brucella infection. mice were immunized subcutaneously with heat-killed ochrobactrum anthropi, sinorhizobium meliloti, mesorhizobium loti, agrobacterium tumefaciens, or brucella melitensis h38 (standard positive control) before intravenous challenge with brucella abortus 2308. cross ... | 2007 | 17715332 |
| blue-light-activated histidine kinases: two-component sensors in bacteria. | histidine kinases, used for environmental sensing by bacterial two-component systems, are involved in regulation of bacterial gene expression, chemotaxis, phototaxis, and virulence. flavin-containing domains function as light-sensory modules in plant and algal phototropins and in fungal blue-light receptors. we have discovered that the prokaryotes brucella melitensis, brucella abortus, erythrobacter litoralis, and pseudomonas syringae contain light-activated histidine kinases that bind a flavin ... | 2007 | 17717187 |
| toxic-febrile neurobrucellosis, clinical findings and outcome of treatment of four cases based on our experience. | central nervous system (cns) involvement is uncommon in brucellosis. neurological complications of brucellosis may be divided into 2 major groups: 1) those related to the acute-febrile state that occurs in acute disease (toxic-febrile neurobrucellosis), and 2) those related to actual invasion and localization of the pathogen in the cns (classical neurobrucellosis). in this paper, we present 4 cases of toxic-febrile neurobrucellosis and discuss clinical findings and outcome of treatment in these ... | 2007 | 17852920 |
| brucella peritonitis and leucocytoclastic vasculitis due to brucella melitensis. | brucellosis is a multisystemic disease that rarely leads to a fatal outcome. while reticuloendothelial system organs are mostly affected, peritonitis and posthepatitic cirrhosis are also complications of brucellosis, though they are very rare. brucella spp. can also trigger immunological reactions. we report a case of brucellosis with peritonitis, renal failure and leucocytoclastic vasculitis caused by brucella melitensis, which led to a fatal outcome. brucellosis should be considered in the dif ... | 2007 | 17874004 |
| aerosol infection of balb/c mice with brucella melitensis and brucella abortus and protective efficacy against aerosol challenge. | brucellosis is a zoonotic disease with a worldwide distribution that can be transmitted via intentional or accidental aerosol exposure. in order to engineer superior vaccine strains against brucella species for use in animals as well as in humans, the possibility of challenge infection via aerosol needs to be considered to properly evaluate vaccine efficacy. in this study, we assessed the use of an aerosol chamber to infect deep lung tissue of mice to elicit systemic infections with either bruce ... | 2007 | 17664263 |
| [isolation of brucella melitensis from ejaculate culture of a brucellosis patient with epididymoorchitis]. | brucellosis is a significant public health problem particularly in developing countries. people are frequently infected through milk, milk products, urine and pregnancy material of animals with brucellosis. epididymoorchitis is the most frequent genitourinary complication of brucellosis and is often unilateral. in this report, a 35 years old male patient who was diagnosed as epididymoorchitis based on clinical presentation, laboratory findings and imaging techniques, has been presented. brucella ... | 2007 | 17933260 |
| the three-dimensional crystal structure of the prpf protein of shewanella oneidensis complexed with trans-aconitate: insights into its biological function. | in bacteria, the dehydration of 2-methylcitrate to yield 2-methylaconitate in the 2-methylcitric acid cycle is catalyzed by a cofactor-less (prpd) enzyme or by an aconitase-like (acnd) enzyme. bacteria that use acnd also require the function of the prpf protein, whose function was previously unknown. to gain insights into the function of prpf, the three-dimensional crystal structure of the prpf protein from the bacterium shewanella oneidensis was solved at 2.0 a resolution. the protein fold of p ... | 2007 | 17567742 |
| molecular epidemiological and antibiotic susceptibility characterization of brucella isolates from humans in sicily, italy. | brucellosis is a serious problem in sicily. brucella melitensis was identified as the species most frequently isolated in humans in italy. no data, however, are available about the molecular epidemiological characterization of brucella isolates from humans. we have conducted this study to molecularly characterize clinical isolates of brucella spp. and to evaluate their antimicrobial susceptibilities. twenty brucella isolates were studied. differential growth characteristics and dna polymorphisms ... | 2007 | 17634297 |
| brucellosis in a returned traveler and his wife: probable person-to-person transmission of brucella melitensis. | 2007 | 17883467 | |
| an aerosolized brucella spp. challenge model for laboratory animals. | to characterize the optimal aerosol dosage of brucella abortus strain 2308 (s2308) and b. melitensis (s16m) in a laboratory animal model of brucellosis, dosages of 10(3)-10(10) colony forming units (cfu) were nebulized to mice. although tissue weights were minimally influenced, total cfu per tissues increased beginning at 10(6)-10(7) cfu dosages, with 10(9) cfu appearing to be an optimal dosage for s16m or s2308 aerosol delivery. at 12 weeks after vaccination with 10(7) cfu of b. abortus strain ... | 2007 | 17894637 |
| review of clinical and laboratory features of human brucellosis. | infection with brucella spp. continues to pose a human health risk globally despite strides in eradicating the disease from domestic animals. brucellosis has been an emerging disease since the discovery of brucella melitensis by sir david bruce in 1887. although many countries have eradicated b. abortus from cattle, in some areas b. melitensis and b. suis have emerged as causes of this infection in cattle, leading to human infections. currently b. melitensis remains the principal cause of human ... | 2007 | 17901634 |
| uptake of ciprofloxacin and ofloxacin by 2 brucella strains and their fluoroquinolone-resistant variants under different conditions. an in vitro study. | the existence of an efflux-mediated mechanism involved in the drug accumulation process of the 2 resistant brucella strains was investigated; 2 susceptible brucella strains, brucella melitensis biovar abortus 2308 (s2308) and afbortus 3196 (s3196) strains, were the reference strains cultured on trypticase soy agar (tsa) medium and incubated to gradient concentrations of ciprofloxacin and ofloxacin antibiotics. the uptake level of 2 antibacterial agents in 2 susceptible and their fluoroquinolones ... | 2007 | 17920802 |
| valve replacement for brucella endocarditis: two case reports. | we report two cases of successful treatment of brucella endocarditis. both of them were treated with antibiotics and aortic valve replacement after brucellosis was diagnosed. in one of these cases emergency operation was required. our observations suggest that a combined surgical and medical treatment is the best option for the management of this disease. b. endocarditis should be operated after improvement of clinical status but emergency cardiac surgery may be required if heart failure develop ... | 2008 | 17651833 |
| validation of a competitive elisa for diagnosis of brucella melitensis infection in sheep and goats. | a competitive enzyme-linked immunosorbent assay (celisa) was validated for the serodiagnosis of brucella melitensis infection in small ruminants using 2108 positive and 2154 negative reference sera from sheep and goats. the optimum cut-off values, offering the highest diagnostic sensitivity (dsn) and diagnostic specificity (dsp), determined by receiver operating characteristic analysis, were at 23.6%, 21.8% and 25.0% inhibition of the conjugate control for sheep, goats and both species, respecti ... | 2008 | 17574884 |
| cytotoxicity in macrophages infected with rough brucella mutants is type iv secretion system dependent. | smooth brucella spp. inhibit macrophage apoptosis, whereas rough brucella mutants induce macrophage oncotic and necrotic cell death. however, the mechanisms and genes responsible for brucella cytotoxicity have not been identified. in the current study, a random mutagenesis approach was used to create a mutant bank consisting of 11,354 mutants by mariner transposon mutagenesis using brucella melitensis rough mutant 16m delta manba as the parental strain. subsequent screening identified 56 mutants ... | 2008 | 17938217 |
| brucellosis of the lung: case report and review of the literature. | human brucellosis is a worldwide re-emerging zoonosis. however, its histological appearance has only been occasionally described. we report the case of a young girl who had been suffering from a spontaneous fracture of the eighth thoracic vertebra at the age of 7. at the age of 15, x-ray showed a translucence of the seventh and ninth thoracic vertebra, and additionally, a bi-lateral episcleritis was detected. three months later, she was admitted to the hospital because of perspiration at night a ... | 2008 | 17952458 |
| comparison of seven commercial dna extraction kits for the recovery of brucella dna from spiked human serum samples using real-time pcr. | we compared the relative recovery of extraction of bacterial dna from serum using seven commercial kits (ultraclean dna bloodspin kit, puregene dna purification system, wizard genomic dna purification kit, high pure pcr template preparation kit, gfx genomic blood dna purification kit, nucleospin tissue kit, and qiaamp dna blood mini kit). human serum samples were spiked with known concentrations of brucella melitensis rev 1; the dna was extracted and tested in genus-specific lightcycler polymera ... | 2008 | 17973130 |
| rapid diagnosis of human brucellosis by quantitative real-time pcr: a case report of brucellar spondylitis. | blood samples and a bone biopsy specimen from one patient diagnosed with spondylodiscitis of unknown etiology were analyzed by quantitative real-time pcr to detect brucella melitensis. the high sensitivity and specificity of this assay allowed the diagnosis of brucellar spondylitis within 24 h, a result that we were unable to obtain through the use of conventional methods. | 2008 | 17977995 |
| bacteremia is as unpredictable as clinical manifestations in human brucellosis. | because of the suboptimal recovery rate of brucellae from blood, it has been proposed that cultures of bone marrow, liver tissue, and lymph nodes may improve the recovery rate of the organism. data in support of these recommendations are limited and not clearly convincing, especially that of bone marrow culture. the main purpose of this work was to evaluate the roles of blood, bone marrow, liver, and lymph node cultures in the diagnosis of human brucellosis. | 2008 | 18023384 |
| the bmei0216 gene of brucella melitensis is required for internalization in hela cells. | brucella is an intracellular facultative bacterium able to survive and multiply in professional and non-professional phagocytes. however, its adhesion and invasion mechanisms have not been elucidated yet. in this work, we assess the interruption of a bmei0216 gene of brucella melitensis, by using hela epithelial cells and murine macrophages for invasion and replication assays. the mutation did not affect survival or multiplication within macrophages. likewise, invasion assays with hela cells rev ... | 2008 | 17881185 |
| hepatic abscess: a rare manifestation of brucellosis in children. | hepatic abscess caused by brucellosis is extremely rare in children. we report the case of a 5-year-old girl in whom an abscess of the liver developed during an episode of acute brucellosis. to our knowledge, this is the second reported case of hepatic abscess caused by brucellosis in a child. | 2008 | 17618456 |
| a 70-year-old stock-breeder with tricuspid valve and defibrillator lead brucella endocarditis. | brucella endocarditis is a rare and life threatening complication of brucellosis. it usually involves the aortic valve and successful management requires a combination of medical treatment and valve replacement. we describe a case of tricuspid valve and defibrillator lead brucella endocarditis induced by the implantation of the defibrillator itself. our patient was admitted to hospital with a 2-week history of fever, back pain and night sweats. one month prior to admission, due to episodes of sy ... | 2008 | 17395320 |
| the effects of increased camp content on inflammation, oxidative stress and pde4 transcripts during brucella melitensis infection. | cyclic amp (camp) is a key intracellular second messenger which at increased levels has been shown to have anti-inflammatory and tissue-protective effects. its concentration is determined by the activities of both adenylate cyclase (ac) and the phosphodiesterase (pde) enzymes. the aim of this study was to compare the effects of increased camp and glucocorticoid dexamethasone administration on b. melitensis-induced lipid peroxidation, brucella suppressed antioxidant enzyme activities and pde4 tra ... | 2008 | 17397885 |
| a case of disseminated intravascular coagulation caused by brucella melitensis. | although the haematological abnormalities were common accompaniments of brucellosis, overt disseminated intravascular coagulopathy was reported only in a case at the literature. we report here a case of brucella melitensis sepsis which showed an acute onset with clinical and hematological findings disseminated intravascular coagulopathy | 2008 | 17562127 |
| kinetic study of cytokines production by human peripheral blood mononuclear cells in response to brucella dna. | in spite of reports on cytokines induction by the brucella dna in murine model, there is no comparison between pathogenic and appropriate vaccine strains in human. we investigated the cytokines profile of human peripheral blood mononuclear cells (pbmcs) induced by dna extracted from pathogenic isolates of brucella melitensis and b. abortus as well as rev1 and s19; the appropriate vaccine strains. it was observed that despite differential induction of interleukin(il)-12 and il-10 production, iden ... | 2008 | 17008080 |
| ventriculo-peritoneal shunt infection caused by brucella melitensis. | 2008 | 19068521 | |
| [sacroilitis and osteomyelitis of the humeral bone du to brucella melitensis in an adolescent]. | we describe a case of a 10 year old patient who presented with intermittent fever and pain in the pelvis and elbow region. from the history, imaging and laboratory tests, a diagnosis of brucella sacroiliitis and elbow osteoarthritis was made. the patient was given an antibiotic treatment for 3 months with a progressive improvement of symptoms to complete recovery, and normalization of imaging and laboratory findings. | 2008 | 19180835 |
| in vitro activity of tigecycline, tetracycline and fluoroquinolones against brucella melitensis. | brucellosis is a major zoonotic disease of global importance. forty years after active drug therapy was defined, there is no optimum antibiotic therapy for brucellosis due to relatively high rates of relapse and treatment failure. the aim of this study was to assess the in vitro susceptibility of 60 brucella melitensis isolates obtained from blood and fluids to tigecycline, tetracycline and three quinolones (ciprofloxacin, levofloxacin, moxifloxacin). the minimum inhibitory concentrations (mics) ... | 2008 | 18390405 |
| severe pharyngitis in stockbreeders: an unusual presentation of brucellosis. | abstract brucellosis is a known occupational hazard for shepherds, abattoir workers, veterinarians, dairy industry professionals and personnel in microbiological laboratories. any organ may be affected by brucella species but to date, severe manifestations in the pharynx have never been reported as the prevailing features of brucellosis. we report two cases in stockbreeders who presented with high-grade fever and severe exudative pharyngitis accompanied by severe odynophagia in the first and wit ... | 2008 | 18397911 |
| a study on the use of male animal models for developing a live vaccine for brucellosis. | to study the safety of brucella melitensis wr201, a live vaccine candidate, we compared the course of infection of this strain with that of virulent 16m in male balb/c mice. at various times after oral immunization with strains wr201 or 16m, lungs, liver, spleen, testis, epididymis, inguinal and cervical lymph nodes were removed. tissues were divided for microbiologic culture and histopathological examination. wr201 infection in male balb/c mice had lower intensity and shorter duration than infe ... | 2008 | 18405337 |
| multicenter study of brucellosis in egypt. | brucellosis causes appreciable economic losses in livestock. examination of milk and tissues from animals in egypt for brucella spp. showed increased prevalence rates of serologically reactive animals. all isolates were b. melitensis biovar 3. one brucella sp. was isolated from milk of serologically nonreactive buffaloes. | 2008 | 19046520 |
| acute brucellosis as a cause of infective colitis. | gastroenterological manifestations of human brucellosis are relatively uncommon. a case of brucella melitensis infection in a 39-year-old man accompanied by lesions of the colon, observed by colonoscopic and histopathologic examinations, and a brief review of the relevant literature are reported. | 2008 | 19055193 |
| brucellosis in india - a review. | brucellosis is an important re-emerging zoonosis with a worldwide distribution. it is still an uncontrolled serious public health problem in many developing countries including india. brucellosis in india is yet a very common but often neglected disease. currently, brucella melitensis accounts for most recorded cases globally with cattle emerging as a important reservoir with the few cases of b. suis. isolated cases of non-terrestrial brucellosis and continuing transmission from wild animals hav ... | 2008 | 19208979 |
| identification, characterization, and structure/function analysis of a corrin reductase involved in adenosylcobalamin biosynthesis. | vitamin b(12), the antipernicious anemia factor, is the cyano derivative of adenosylcobalamin, which is one of nature's most complex coenzymes. adenosylcobalamin is made along one of two similar yet distinct metabolic pathways, which are referred to as the aerobic and anaerobic routes. the aerobic pathway for cobalamin biosynthesis proceeds via cobalt insertion into a ring-contracted macrocycle, which is closely followed by adenosylation of the cobalt ion. an important prerequisite for adenosyla ... | 2008 | 18263579 |
| use of mlva-16 typing to trace the source of a laboratory-acquired brucella infection. | 2008 | 18289724 | |
| brucellar meningitis in an infant--evidence for human breast milk transmission. | brucellar meningitis is relatively uncommon, especially in children younger than 1 year. we present another case of meningitis due to brucella melitensis biotype 1 in a 11-month-old infant transmitted by breast milk. this is the first report of successful isolation of b. melitensis from the breast milk in the literature. babies of infected nursing mother should be monitored closely for evidence of infection since the breast milk is the source of transmission of brucellosis. it would be prudent t ... | 2008 | 18292100 |
| putative quorum-sensing regulator blxr of brucella melitensis regulates virulence factors including the type iv secretion system and flagella. | brucella melitensis is an intracellular pathogen that establishes a replicative niche within macrophages. while the intracellular lifestyle of brucella is poorly understood and few virulence factors have been identified, components of a quorum-sensing pathway in brucella have recently been identified. the luxr-type regulatory protein, vjbr, and an n-acylhomoserine lactone signaling molecule are both involved in regulating expression of the virb-encoded type iv secretion system. we have identifie ... | 2008 | 18310341 |
| subversion of toll-like receptor signaling by a unique family of bacterial toll/interleukin-1 receptor domain-containing proteins. | pathogenic microbes have evolved sophisticated molecular strategies to subvert host defenses. here we show that virulent bacteria interfere directly with toll-like receptor (tlr) function by secreting inhibitory homologs of the toll/interleukin-1 receptor (tir) domain. genes encoding tir domain containing-proteins (tcps) were identified in escherichia coli cft073 (tcpc) and brucella melitensis (tcpb). we found that tcpc is common in the most virulent uropathogenic e. coli strains and promotes ba ... | 2008 | 18327267 |
| unusual manifestation of brucellosis: liver abscess and pancytopenia caused by brucella melitensis. | brucellosis is a zoonosis with a worldwide distribution, and still endemic in greece. although hepatic involvement is common, cholecystitis and hepatic abscesses represent rare complications of the disease. hematological manifestations of brucellosis include various abnormalities such as anemia and leucopenia, but the disease rarely presents with pancytopenia. we present a case of liver abscess and pancytopenia caused by brucella melitensis in a 54-year-old worker who consumed unpasteurized dair ... | 2008 | 18334880 |
| brucellosis in camels (camelus dromedarius) in darfur, western sudan. | in a field outbreak of brucellosis in 21 camels mixed with cattle, sheep and goats, five camels, three of which showed clinical signs, were serologically positive. in a subsequent abattoir survey of apparently healthy camels, six animals were seropositive, albeit with titres that tended to be lower than those found in the field outbreak. of the six seropositive slaughtered camels, five were shown to have lymph nodes (prescapular and supramammary) infected with brucellae (brucella melitensis biov ... | 2008 | 18346482 |
| brucella melitensis b115-based complement fixation test to detect antibodies induced by brucella rough strains. | to assess the efficiency of a brucella melitensis b115 rough strain, naturally devoid of anticomplementary activity, used as antigen in a complement fixation test (cft) to detect antibodies induced by brucella strains with rough phenotype, such as brucella abortus rb51, brucella ovis and brucella canis. | 2008 | 18355234 |
| improved performance of brucella melitensis native hapten over brucella abortus ops tracer on goat antibody detection by the fluorescence polarization assay. | the current method for goat brucellosis diagnosis is based on the world organization for animal health (oie) using the screening card test (ct), with antigen at 8% (ct8) or 3% (ct3) of cell concentrations, and the confirmatory complement fixation test (cft). however, these tests do not differentiate antibodies induced by vaccination from those derived from field infections by brucella species or other bacterial agents; in places like mexico, where the prevalence of brucellosis and the vaccinatio ... | 2008 | 18359093 |
| panniculitis as the initial manifestation of brucellosis: a case report. | here, we describe a 38-year-old male with an abrupt manifestation acute panniculitis as unusual presentation of brucellosis. brucellosis is a reemerging disease in turkey, and the disease is primarily transmitted from farm animals to humans. farmers and shepherds are the major risk groups for brucellosis in anatolia. brucellosis may involve almost all systems and organs, including the skin, and may mimic a wide range of illness and syndromes. although the cutaneous manifestation of brucellosis i ... | 2008 | 18360123 |
| immunization with a single dose of a microencapsulated brucella melitensis mutant enhances protection against wild-type challenge. | the development of safe and efficacious immunization systems to prevent brucellosis is needed to overcome the disadvantages of the currently licensed vaccine strains that restrict their use in humans. alginate microspheres coated with a protein of the parasite fasciola hepatica (vitelline protein b [vpb]) and containing live brucella melitensis attenuated mutant vjbr::tn5 (bmeii1116) were evaluated for vaccine efficacy and immunogenicity in mice. a single immunization dose in balb/c mice with th ... | 2008 | 18362129 |
| brucella infection in total knee arthroplasty. case report and revision of the literature. | brucella infection in arthroplasties is a rare event. an unspecific clinical symptomatology is associated with unclear radiographic peri-prosthetic signs of bone halisteresis. only a positive anamnesis, combined with an antibiogram of the joint liquid and a high serum antibody titre, can lead to a definitive diagnosis. we report a case of brucella melitensis infection in a total knee arthroplasty implanted 2 years earlier. with the absence of radiological signs of prosthetic loosening and thanks ... | 2008 | 18369535 |
| [consider brucellosis if the patient suffers of protracted fever! visiting middle east a high-risk factor]. | 2008 | 18376709 | |
| use of the brucella melitensis native hapten to diagnose brucellosis in goats by a rapid, simple, and specific fluorescence polarization assay. | the performance of the fluorescence polarization assay (fpa) using the recently described brucella melitensis native hapten and the brucella abortus o-polysaccharide tracer was evaluated and compared with those of the world organization for animal health tests related to indirect and competitive enzyme-linked immunosorbent assays as classification variables for goat sera obtained from a high-prevalence area where vaccination was performed; test series were also evaluated to increase the final sp ... | 2008 | 18385457 |
| ocular manifestations associated with brucellosis: a 26-year experience in peru. | brucellosis has unusual clinical manifestations. ocular involvement caused by brucellosis remains poorly recognized in areas in which brucellosis is endemic. | 2008 | 18419433 |
| immunopathological responses and kinetics of brucella melitensis rev 1 infection after subcutaneous or conjunctival vaccination in rams. | the innocuousness of the brucella melitensis rev 1 live attenuated vaccine strain has never been fully assessed in rams. the immunopathological responses and the kinetics and distribution of the infection induced by this strain were determined after subcutaneous or conjunctival vaccination in both young (3-4 months old) and adult (12 months old) rams. at regular intervals after vaccination the animals were bled for serological studies, and slaughtered for both pathological and bacteriological ex ... | 2008 | 18423950 |
| ultraviolet c lethal effect on brucella melitensis. | the gram-negative bacteria brucella melitensis was investigated to evaluate its susceptibility to uvc radiation at 254 nm. at an intensity of 18.7 mw/cm2 of uvc, the time required for inactivation of b. melitensis was 240 seconds in both dark and light, whereas it was 120 seconds and 240 seconds in dark and light respectively at an intensity of 19.5 mw/cm2. the results indicate that vaccinal strain of b. melitensis (rev.1) is more sensitive to uvc than wild b. melitensis strain. | 2008 | 18437841 |
| [short communication: investigation of in vitro antibiotic susceptibility of brucella melitensis]. | the aim of this study was to investigate the in vitro susceptibilities of brucella melitensis clinical isolates against antibiotics used in conventional treatment regimens and additionally against quinolones. a total of 65 b. melitensis strains isolated from blood and bone marrow specimens of patients who were admitted to an university hospital localized in a region endemic for brucellosis. minimum inhibitory concentration values (mic50 and mic90) of trimethoprim-sulphamethoxazole (tmp-smx), rif ... | 2008 | 18444570 |
| diagnostic and therapeutic challenges of childhood brucellosis in a nonendemic country. | we sought [corrected] to define the current epidemiology, clinical manifestations, and course of childhood brucellosis in the united states. | 2008 | 18450861 |
| an eternal microbe: brucella dna load persists for years after clinical cure. | despite the continuing high incidence of brucellosis, vague aspects of pathophysiology, diagnosis, and treatment continue to exist, particularly with regard to the ability of brucella species to survive inside the host. | 2008 | 18462106 |
| risk factors associated with camel brucellosis in jordan. | during the period between february, 2004 and december, 2006, a cross-sectional study was performed to investigate some epidemiological aspects related to camel brucellosis in jordan. four hundred twelve camel sera from 37 herds were randomly collected and analyzed using rose bengal plate test and complement fixation test. a structured pre-tested questionnaire was administered to collect information on camel herd health and management. a multivariable logistic regression model was constructed to ... | 2008 | 18484121 |
| cold agglutinin-associated hemolytic anemia due to brucellosis: first case report. | 2008 | 18512765 | |
| intracellular rescuing of a b. melitensis 16m virb mutant by co-infection with a wild type strain. | brucella is a broad-range, facultative intracellular pathogen that can survive and replicate in an endoplasmic reticulum (er)-derived replication niche by preventing fusion of its membrane-bound compartment with late endosomes and lysosomes. this vacuolar hijacking was demonstrated to be dependent on the type iv secretion system virb but no secreted effectors have been identified yet. a virb mutant is unable to reach its er-derived replicative niche and does not multiply intracellularly. in this ... | 2008 | 18547782 |
| [laboratory-acquired brucellosis]. | brucellosis is a rare disease in denmark. we describe one case of laboratory-acquired brucellosis from an index patient to a laboratory technician following exposure to an infected blood culture in a clinical microbiology laboratory. | 2008 | 18565306 |
| in vitro-selected resistance to fluoroquinolones in two brucella strains associated with mutational changes in gyra. | in the present study, brucella melitensis biovar abortus 2308 and brucella abortus 3196 biotype 5 reference strains, which are susceptible to fluoroquinolones, became in vitro-resistant to fluoroquinolones by culture in trypticase soy agar. the quinolone resistance-determining regions (qrdrs) of the gyra and parc genes of the two reference strains were analysed by polymerase chain reaction sequencing analysis to obtain the wild-type sequence. these sequences were then compared with the correspon ... | 2008 | 18583100 |
| virb12 is a serological marker of brucella infection in experimental and natural hosts. | the brucella species type iv secretion system, encoded by the virb1-12 locus, is required for intracellular replication and persistent infection in vivo. the requirement of virb proteins for infection suggests that they are expressed in vivo and may therefore represent serological markers of infection. to test this idea, we purified recombinant virb1, virb5, virb11, and virb12 and tested for their recognition by antibodies in sera from experimentally infected mice and goats by using an indirect ... | 2008 | 18077620 |
| splenic puncture: diagnostic accuracy and safety in infectious diseases. | 2008 | 18082440 | |
| specific detection and differentiation of ochrobactrum anthropi, ochrobactrum intermedium and brucella spp. by a multi-primer pcr that targets the reca gene. | ochrobactrum anthropi, ochrobactrum intermedium and brucella spp. are phenotypically and genetically closely related pathogens that may cause disease with similar clinical presentation. consequently, difficulties in their identification and differentiation have been reported. in this study, a sensitive reca gene-based multi-primer single-target pcr (mp-st-pcr) was developed that allowed the specific detection and differentiation of these clinically relevant pathogens. the specificity of the assa ... | 2008 | 18065669 |
| brucellosis in cases of end-stage renal disease. | patients with brucellosis frequently present with joint and bone pain. however, brucellosis may be overlooked in patients with end-stage renal disease (esrd) who undergo dialysis since amyloidosis due to renal osteodystrophy and beta-2 microglobulinaemia may cause bone pain as well. only four cases of end-stage renal failure accompanied by brucellosis have been reported in the literature. we evaluated clinical and laboratory characteristics and organ involvement of seven brucellosis patients wit ... | 2008 | 18178600 |
| comparison of culture and pcr for the detection of brucella melitensis in blood and lymphoid tissues of serologically positive and negative slaughtered sheep. | to compare the culture and pcr methods for detection of brucella melitensis in blood and lymphoid tissue samples obtained from slaughtered sheep (n = 162) testing positive/negative in serological tests (rose bengal test and serum agglutination test). | 2008 | 18179446 |
| design and implementation of a database for brucella melitensis genome annotation. | the genome sequences of three brucella biovars and of some species close to brucella sp. have become available, leading to new relationship analysis. moreover, the automatic genome annotation of the pathogenic bacteria brucella melitensis has been manually corrected by a consortium of experts, leading to 899 modifications of start sites predictions among the 3198 open reading frames (orfs) examined. this new annotation, coupled with the results of automatic annotation tools of the complete genom ... | 2008 | 18160234 |
| brucella glomerulonephritis resulting in end-stage renal disease: a case report and a brief review of the literature. | brucella glomerulonephritis is a rare condition with only a few reported cases. we review the literature, and describe a 24-year-old female who presented with edema and proteinuria. blood grew brucella melitensis. renal biopsy showed diffuse proliferative glomerulonephritis. the patient progressed to end-stage renal disease despite antibiotic and steroid therapy. | 2008 | 18196469 |
| non-imported brucellosis outbreak from unpasteurized raw milk in moroccan immigrants in spain. | nine cases of brucellosis were identified in the city of elche (comunidad valenciana, spain) in two families of moroccan immigrants. all of the patients had drunk unpasteurized raw milk from goats. brucella melitensis biovar 3 was identified in clinical specimens. preventive measures for brucellosis should be implemented among immigrant populations in spain. | 2008 | 18205974 |