Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [viruses in the feces of patients with viral hepatitis and other enterovirus infections]. | direct and immune electron microscopy was used to determine the frequency of finding of hepatitis a virus (hav) and other viral agents in feces of patients in relation to the diagnosis and epidemiological situation. hav-containing excretions from patients were analysed ultrastructurally. the highest frequency of hav detection was established in patients in a water-borne and food-borne outbreak of hepatitis a (ha) and was 40.9% and 36.9%, respectively. in patients with ha diagnosis in the period ... | 1984 | 6097043 |
| [immunofluorescent study of the reproduction of human rotavirus in cell culture]. | the mechanism of rotavirus antigen amplification in cell culture was studied by immunofluorescence. reproduction of human rotavirus in cell culture was shown to begin 6 h after inoculation and to be accompanied by a regular increase in the number of antigen-containing cells. a good correlation with the results of electron microscopic studies was demonstrated. the possibility of reliable detection of rotavirus antigen in cell culture by the fluorescent antibody technique at relatively early inter ... | 1984 | 6097047 |
| [detection of rotavirus in the feces of children with diarrhea]. | bacteriological and virological examinations of feces from sick children diagnosed as having an "enteric infection of obscure etiology" were carried out. out of 30 fecal specimens 6 showed positive results in agar gel diffusion test with antiserum to calf diarrhea rotavirus confirmed by electron microscopy. the relationship of virological and bacteriological findings and age of the patients was established. differences in the clinical manifestations of the disease in virus-positive and virus-neg ... | 1984 | 6097048 |
| synergism in experimental mixed infections of newborn colostrum-deprived calves with bovine rotavirus and enterotoxigenic escherichia coli (etec). | 1984 | 6097066 | |
| vaccination of cows with k 99 and rotavirus antigen: potency of k 99 antigen combined with different adjuvants in stimulating milk antibody secretion. | 1984 | 6097069 | |
| pathogenesis and immunoprophylaxis of bovine rotavirus infection. | 1984 | 6097086 | |
| infectious agents associated with diarrhoea in commercial rabbits: a field study. | over a period of 9 months the occurrence of parasites, bacteria and viruses has been examined in diarrhoeic rabbits from 21 commercial rabbitries and related with clinical signs and gross and microscopic lesions. infectious disease agents have been found in 71.5% of the animals. escherichia coli (atec) were found to be attached to the luminal intestinal border of 40% of the rabbits examined. this was associated with moderate to high mortality, caecal oedema, severe swelling of mesenteric lymph n ... | 1984 | 6097152 |
| [experimental infection of fattening swine with rotavirus: study of local immunity using coproantibodies]. | fattened pigs were experimentally infected with porcine rotavirus (osu strain). viral antigens found in the faeces were analysed over a period of two months (elisa method). we have found no correlation between virus excretion and the clinical manifestation of disease. the local immunity has been analysed in the faeces by the coproantibodies antirotavirus. the different classes of antibody, g, m, a and immune complexes have been determined using the elisa method. the level of iga antibodies was h ... | 1984 | 6097155 |
| [viral etiology of gastroenteritis]. | 1984 | 6097282 | |
| epidemiology of rotavirus gastroenteritis in infants from the area of bochum as revealed by electrophoresis of genome rna. | rotaviruses were detected by enzyme-linked immunosorbent assay (elisa) in stool samples from 504 infants admitted with gastroenteritis to the children's hospital of the university bochum in the period from 1979 to 1984. in most infants rotaviruses persisted in the stools for 6-8 days. rotavirus rna was extracted from stool samples from 203 infants. by acrylamide gel electrophoresis, bands characteristic of rotavirus double-stranded rna were detected in 172 (85%) of these 203 samples in which the ... | 1984 | 6097451 |
| [new developments in rotavirus research]. | 1984 | 6097517 | |
| isolation of a rotavirus from canine diarrheal feces. | 1984 | 6097734 | |
| [acute gastroenteritis and breast feeding in gabon. preliminary results]. | 76 diarrheic infants (from 15 days to 2 years) had a stool examination for rotavirus (elisa method) and a stool culture and were classified according to breast feeding or not. this study was performed in a semi-urban region (haut-ogooué, gabon). the preliminary results suggest a protection by breast feeding against rotavirus and enteropathogenic bacteria, rapid elimination of diarrheic agents may be facilitated by human milk. | 1984 | 6097788 |
| an attempt to cultivate human rotavirus in human leukocytes culture (preliminary report). | 1984 | 6097999 | |
| rotavirus in pediatric diarrhoea at siriraj hospital, bangkok, thailand. | between june 1982 and may 1983, rotavirus was found in 29% of 248 pediatric patients between 1 month to 5 years of age at siriraj hospital, bangkok, thailand. among 413 household contacts, rv was detected in 9 (2%) of whom 6 developed diarrhoea. rv was not found in age-matched healthy controls. in a serological study, the serological response was 81% in rotavirus diarrhoea cases (17 out of 21) by elisa. by the elisa blocking test, the seroconversion rate was only 29.6% in rotavirus diarrhoea cas ... | 1984 | 6098022 |
| "enveloped" rotavirus particles detected in culture fluid. | in a negative contrast preparation of human rotavirus culture fluid were found membrane-bound "enveloped" virus particles which had been observed only in thin sections of the infected cells. the "enveloped" particles comprised a single-shelled particle and degenerating membrane structure. | 1984 | 6098053 |
| [enteropathogenicity of bovine coronaviruses under field conditions]. | 1984 | 6098103 | |
| studies on the growth of bovine rotavirus in cell cultures. | 1984 | 6098104 | |
| comparison of three enzyme-linked immunosorbent assays suitable for the detection of antibodies to rotaviruses in epidemiological studies. | three enzyme-linked immunosorbent assays which use commercially available reagents are presented as alternatives to the complement fixation procedure for large-scale detection of rotavirus antibodies. comparison of results in 75 sera tested by indirect, competition and blocking elisa and by complement fixation demonstrated that the elisa techniques were rapid, easy to perform and 26 to 29 times more sensitive than complement fixation; furthermore the elisa techniques permitted extrapolation of a ... | 1984 | 6098473 |
| a bovine rotavirus developed as a candidate vaccine for use in humans. | 1984 | 6098585 | |
| the efficacy of colostrum from cows vaccinated with rotavirus in protecting calves to experimentally induced rotavirus infection. | calves which were continuously fed colostrum from vaccinated cows for the first ten days of life, were fully refractory to experimental infection with strain 81/36 f of bovine rotavirus. by contrast, the response to virus exposure of calves which were treated with normal colostrum was identical to that of the control calves, in that they underwent severe diarrhea and a significant slowing of the growth rate. the antibody titer in the milk of vaccinated cows tends to decline rapidly so that it no ... | 1984 | 6086224 |
| prevalence of antibody to rotavirus in moroccan cattle. | a serological survey was carried out in order to determine the prevalence of antirotavirus antibodies in moroccan cattle under different management conditions. from the 493 serum samples examined, 325 (65.9%) were found positive, using a counter-immunoelectroosmophoresis technique. animals of indigenous breed coming from farms with rapid turnover or large number of animals, or having frequent contacts with imported cattle, had a higher rate of seropositivity; however, positive sera were also fou ... | 1984 | 6086226 |
| an improved enzyme-linked immunosorbent assay for the detection of rotavirus in faeces of neonates. | a direct enzyme-linked immunosorbent assay (eia) for the detection of rotavirus in neonatal stools was developed. rabbit antiserum against sa 11 rotavirus was incorporated as both coating and detector antibody, and rotavirus-negative rabbit serum was applied as a coating antibody control to eliminate false positive results. pretreatment of stools with edta was found to increase both the sensitivity and specificity of the assay. this effect was greatest when 0.25 m edta (tetrasodium salt) was inc ... | 1984 | 6086688 |
| inactivation of human rotavirus, sa11 and other enteric viruses in effluent by disinfectants. | a preparation of infectious human rotavirus, isolated from faeces and resuspended in wastewater effluent, was shown to be inactivated by chlorine, chlorine dioxide, ozone and peracetic acid. infectivity was assayed in ma 104 cells by the detection of cell-associated viral antigen by immunofluorescence. the inactivation curves were similar to those reported for other enteric viruses. human rotavirus was at least as resistant as poliovirus, coxsackievirus, echovirus and f2 coliphage and was striki ... | 1984 | 6086748 |
| the genomes of rotaviruses isolated from chronically infected immunodeficient children. | abnormal rna migration profiles were found in numerous rotavirus samples from two chronically infected children suffering from severe combined immunodeficiency. in both cases additional bands consisting of dsrna were found migrating between rna segments 1 and 7, and in one case rna segment 11 was lost from the profile. hybridization studies using segment-specific cloned cdna probes indicated that some of the additional bands had sequence homologies with normal rotavirus dsrna segments. in most c ... | 1984 | 6086807 |
| antigenic and pathogenic relationships of three bovine rotaviruses and a porcine rotavirus. | the antigenic relationships between three bovine rotaviruses, uk, cp-1 and pp-1, and a porcine rotavirus were investigated; their pathogenicity for piglets was also assessed. after propagation and cloning in cell culture, the four viruses were used to produce convalescent and hyperimmune antisera in gnotobiotic animals. for the uk and pp-1 viruses, reciprocal virus neutralization tests gave ratios of homologous to heterologous titres in the range 57 to greater than 2300. taking a 20-fold differe ... | 1984 | 6086808 |
| oligosaccharide composition of calf rotavirus. | the oligosaccharides of the structural glycoprotein (vp7) of calf rotavirus were characterized. the precursor of vp7 produced in infected cells in the presence of tunicamycin migrated on sds-polyacrylamide gels with an apparent molecular weight 6000 less than the glycosylated glycoprotein. endoglycosidase (endo) h digestion of the mature virus resulted in a decrease of 5000 in the molecular weight of vp7 in two discrete stages. analysis of endo h-treated, 3h-labelled digestion products of vp7 on ... | 1984 | 6086809 |
| propagation of rotaviruses in the presence of chicken serum. | the ability of mammalian rotaviruses to replicate in bsc-1 and cv-1 cell cultures was facilitated by the presence of 4% chicken serum. viral plaques tended to be larger and appear more quickly than in cultures without added chicken serum. it is proposed that chicken serum facilitates plaquing of rotavirus due to its lack of trypsin inhibitors. | 1984 | 6086811 |
| comparison of bovine, simian and human rotavirus structural glycoproteins. | the structural glycoproteins of calf (bdv 486), human (wa) and simian (sa11) rotaviruses were compared for sensitivity to endoglycosidase (endo) h. the calf and human virus glycoproteins were reduced by approximately 5000 molecular weight in two equal stages. the simian rotavirus glycoprotein sustained only one 2500 molecular weight decrease. the effect of the host cell on the viral oligosaccharide composition was examined. endo h digests of calf rotavirus 82-124 extracted from stool and its pro ... | 1984 | 6086812 |
| identification of a bovine rotavirus gene and gene product influencing cellular attachment. | preliminary characterization of naturally occurring temperature-sensitive bovine rotavirus mutants, generated via gene reassortment, indicated that the product of gene segment 10 can influence virus adsorption to ma-104 cells. cell-free translation of this gene segment in a rabbit reticulocyte system was then utilized to identify a polypeptide with a molecular weight of ca. 21,500 as the primary translation product. this identification provides a basis for further studies of rotavirus assembly a ... | 1984 | 6086958 |
| behaviour of campylobacter sputorum subspecies mucosalis in gnotobiotic pigs. | gnotobiotic pigs were dosed orally with campylobacter sputorum subspecies mucosalis, either alone, or combined with rotavirus or non-pathogenic escherichia coli and streptococcus bovis to study the behaviour of c s mucosalis in defined conditions, to assess intracellular parasitism of enterocytes by c s mucosalis, and if possible to establish an experimental model of porcine intestinal adenomatosis. c s mucosalis colonised the gut of gnotobiotic pigs, persisting for up to 47 days after infection ... | 1984 | 6087431 |
| a controlled study of endemic sporadic diarrhoea among adult residents of southern brazil. | the aetiology of sporadic summer diarrhoea and enterotoxin (lt) antibody titres was studied among 43 adult patients in southern brazil who had an acute, untreated diarrhoeal illness and 43 age- and sex-matched controls from the same area. a potential pathogen was identified in 33 of 34 patients and in 17 of 43 controls (p less than 0.01). 10 shigella, one invasive escherichia coli, one salmonella and one entamoeba histolytica were identified in 17 patients with inflammatory diarrhoea (faecal pol ... | 1984 | 6087512 |
| [electron microscopic diagnosis of viral diseases]. | rotaviruses were detected in 58 among 194 children with nonbacterial gastroenteritis whose feces were examined by direct electron microscopy and immune electron microscopy (iem). identification of coxsackie b viruses isolated from patients with infectious-allergic myocarditis by iem and the neutralization tests gave similar results (coxsackie b6). besides, iem detected the accompanying virus types. | 1984 | 6087565 |
| [new type of rotavirus particles found in the stool of patients with adult epidemic diarrhea]. | 1984 | 6088050 | |
| nomenclature of human rotaviruses: designation of subgroups and serotypes. | based on the specificity of subgroup antigens and serotype antigens which are situated, respectively, in the major inner and outer capsid polypeptides, a new nomenclature for human rotaviruses is proposed. the subgroups are designated as i and ii, and the serotypes as 1, 2, 3, 4. | 1984 | 6088101 |
| influence of breast milk on nosocomial rotavirus infections in infants. | to prevent nosocomial rotavirus infections in hospitalized children with various non-gastrointestinal diseases, 30 children (mean age five months) received 200 ml of fresh human milk per day in addition to the normal diet for their age. a matched group of children on formula diet served as a control. fecal samples were routinely screened for rotavirus by a commercial elisa test. in stools containing rotavirus, the virus rna segments were analysed by gel electrophoresis to identify the different ... | 1984 | 6088396 |
| shifts in the electrophoretic pattern on the rna genome of rotaviruses under different electrophoretic conditions. | substantial differences in the rna electrophoretic patterns of rotaviruses were observed when the conditions for gel electrophoresis were varied. electropherotypes which seem alike under one set of conditions, may appear significantly different under other conditions such as distinct proportions of acrylamide and bisacrylamide or different running buffer. different patterns were observed for the same rotavirus rna sample even when different voltages were applied to otherwise identical runs; thes ... | 1984 | 6088565 |
| efficiency of human rotavirus propagation in cell culture. | this study was designed to find methods to reproducibly propagate human rotaviruses from fecal specimens and to determine the relationship between particle numbers and infectivity. growth of virus was initially compared in primary and continuous lines of monkey kidney cells. primary cells (african green and cynomolgus monkey kidney) supported virus growth directly from fecal specimens much more efficiently than did continuous lines of african green (cv-1) or rhesus (ma104) monkey kidney cells. r ... | 1984 | 6088569 |
| detection of rotavirus in human stools by using monoclonal antibody. | a monoclonal antibody, 3f7, that reacts with the common rotavirus antigen on the sixth viral gene product was prepared. it was used in a direct monoclonal antibody radioimmunoassay (ria) as a diagnostic reagent for detection, in 3.5 h, of rotavirus in human pediatric stool specimens. in the 177 samples tested, a concordance of 96% was seen between the monoclonal ria and the well-established and commonly used commercially available rotazyme test. six discrepant specimens that were positive by mon ... | 1984 | 6088573 |
| monoclonal antibodies against bovine immunoglobulins and their use in isotype-specific elisas for rotavirus antibody. | monoclonal antibodies (mca) against bovine immunoglobulin (big) isotypes were produced and characterized. mcas were obtained which react specifically with igg, igg1, igg2 or iga while mcas against igm showed a partial cross-reaction with affinity purified iga. mcas with optimal characteristics for application in elisa were selected and used as conjugates in an indirect double antibody sandwich assay (idas) and as the capturing antibody in an antibody capture assay (aca) for the isotype-specific ... | 1984 | 6088636 |
| nucleotide sequence of human rotavirus genome segment 10, an rna encoding a glycosylated virus protein. | the complete nucleotide sequence of human rotavirus (wa strain) genome segment 10 was determined by using a cloned dna copy. the sequence data indicated that segment 10 is a + t rich (65%) and consists of 750 base pairs. the positive strand of segment 10 contains a single open reading frame that extends 175 codons from the first aug triplet (residues 42 through 44). the amino acid sequence of the segment 10 product was deduced from the nucleotide sequence. there are two distinct glycosylation si ... | 1984 | 6088807 |
| nucleotide sequence of the gene encoding the serotype-specific antigen of human (wa) rotavirus: comparison with the homologous genes from simian sa11 and uk bovine rotaviruses. | the nucleotide sequence of human (wa) rotavirus genome segment 9, which encodes the serotype-specific antigen vp7, has been determined. comparison of the deduced amino acid sequence of wa vp7 protein to the sequences of simian sa11 and uk bovine vp7 proteins shows that the majority of the amino acid differences are clustered between amino acid residues 37 through 49, 65 through 75, 87 through 105, 122 through 126, 146 through 149, 178 through 181, and 208 through 242. a hydrophilicity profile of ... | 1984 | 6088808 |
| [an electron microscopy study of the causes of acute gastroenteritis in children]. | 1984 | 6088921 | |
| [diagnosis of rotavirus infection by isolation of viral rna from feces]. | 1984 | 6089070 | |
| [rotavirus in diarrheas of prolonged course]. | 1984 | 6089275 | |
| prevalence of enteric pathogens in the feces of healthy beef calves. | fecal specimens from 136 healthy beef calves (1 day to 12 weeks of age) were examined for the presence of infectious agents known to cause enteric disease in calves. the calves were selected from 22 herds in which all calves were free of clinically apparent enteric disease. salmonella sp, enterotoxigenic escherichia coli, cryptosporidium, and coronavirus were not detected in any of the calves. three calves were infected with rotavirus and 1 calf was infected with yersinia enterocolitica. campylo ... | 1984 | 6089621 |
| subgrouping of human rotavirus strains by complement fixation, indirect double-antibody sandwich enzyme-linked immunosorbent assay and solid-phase immune electron microscopy. | complement fixation (cf), indirect double-antibody sandwich (das) enzyme-linked immunosorbent assay (elisa) and solid-phase immune electron microscopy (spiem) were compared for their ability to subgroup 73 human rotavirus (hrv) strains from infants and young children with gastroenteritis admitted to one or the other of two different hospitals of northern italy. by both indirect das elisa and spiem all 73 hrv strains were classified into one or the other of two subgroups. by cf only 67 strains co ... | 1984 | 6089702 |
| effect of tunicamycin on human rotavirus morphogenesis and infectivity. brief report. | in the presence of tunicamycin, decreases in the number of double-shelled human rotavirus particles coincided with a reduction in cell lysates infectivity. the majority of particles within the endoplasmic reticulum were membrane bound, "enveloped" particles. | 1984 | 6089709 |
| [virologic and immunologic studies on rotavirus excretion in children]. | 1984 | 6089974 | |
| comparative study of bovine rotavirus isolates by plaque assay. | rotaviruses were isolated on bsc-1 cells from counterimmunoelectrophoresis and/or electron microscopy positive intestinal contents from two asymptomatic and six diarrheic calves from quebec. the plaque assay was performed using these lines and agar overlay medium containing trypsin and deae-dextran. this assay was used to compare the quebec isolates to an attenuated american strain (ncdv) and another strain (th) obtained from france. the ncdv strain produced plaques that were significantly large ... | 1984 | 6089982 |
| prevalence of rotavirus and coronavirus antigens in the feces of normal cows. | the prevalence of rotavirus and coronavirus shedding by adult cows was investigated using capture enzyme-linked immunosorbent assays. fecal samples from 121 cows in a single herd were tested for the presence of rotavirus and coronavirus, either free or complexed with immunoglobulin. free rotavirus was not detected in any samples while rotavirus-immunoglobulin complexes were detected in 53 of 121 (44%) samples tested. in contrast, free coronavirus was detected in six (5%) samples and coronavirus- ... | 1984 | 6089985 |
| the radiographic findings in severe rotavirus-induced colitis. | rotavirus infections are a common cause of childhood gastroenteritis but are rarely severe enough to justify radiographs. however, there appears to be a small subset of children in whom a rotavirus-induced diarrheal illness accompanied by bloody stools is sufficiently severe and protracted to warrant barium studies. we have observed spasm and minute mucosal ulcerations of the colon in three of these children. these findings, under other circumstances, would have prompted the diagnosis of ulcerat ... | 1984 | 6090466 |
| [current virus diseases in horses. diseases in foals and respiratory tract infections]. | at the moment, horse praxis is confronted by two disease complexes which are difficult to fight against as well in prophylaxis as in therapy, but which get an increasing importance. first they concern virus infections of the foals and second primary virus-caused respiratory diseases. foals get infected during the embryonal/fetal development, in the perinatal or postnatal period. normally the infection is caused by latent infected, clinical healthy mares, or in the postnatal period by ubiquitous, ... | 1984 | 6098971 |
| [rotavirus and acute gastroenteritis in children]. | 1984 | 6098983 | |
| [immunofluorescent diagnosis of rota-, corona- and pestivirus infections in calves]. | bovine hyperimmune fluorescent sera were obtained for indication of the rota- and corona-virus infections in calves, which were highly specific and of immunofluorescent titers within the 1:32-64 range. their testing was carried out via the direct immunofluorescence method, using histologic cross-sectioned material and impression preparations of the mucous membrane of calves died of enteritis as well as cell cultures of fetal calf kidney and organic cultures of calf trachea all contaminated, resp ... | 1984 | 6098987 |
| [human rotavirus persistence]. | among 250 tissue specimens collected by aspiration biopsy from the duodenum, small intestine, and rectum of 187 patients, human rotavirus was found in 50 cases in marked cryostate sections by means of fluorescent-serological examinations. at a total rate of findings of 26.7%, rotavirus was repeatedly detected in 5.3% in rebiopsies during more than 10 months, therefore active persistence of human rotavirus must be taken into consideration. | 1984 | 6098998 |
| [light and electron microscopic studies of experimental and spontaneous rotavirus infection in calves]. | 1984 | 6099106 | |
| [comparison of diagnostic methods in rotavirus infection: immunofluorescence, elisa and the latex agglutination test]. | to examine the sensitivity and reliability of a latex agglutination test for detecting rotavirus in faecal samples, we have tested 145 stool specimens from pediatric patients with or without diarrhea, comparing such latex test to immunofluorescent antibody technique (if) and elisa. among all samples positive by if and elisa, 52.9% were positive by latex test. this percentage was raised to 85.7% when only positive samples from patients with diarrhea were considered. we conclude that the latex tes ... | 1984 | 6099580 |
| [virological and bacteriological research on the etiology of gastroenteritis in newborn calves]. | virologic and bacteriologic studies were carried out of samples taken from calves with clinical gastroenteritis and from calves that died at the age of 2 to 10 days. a total of 199 samples were investigated of calves on 48 cattle-breeding farms. in 69 of the cases (34.2 per cent) strains of escherichia coli were isolated, prevailing being those of the o8, o9, o15, o21, o26, o55, and o78 serogroups. three of the strains isolated from fecal samples of clinically affected calves possessed the k99 a ... | 1984 | 6099625 |
| [antirotavirus iga antibody in nasal washes]. | 1984 | 6099628 | |
| ultrastructural localization of rotavirus antigens using colloidal gold. | colloidal gold was used to localize six of the ten known proteins of the simian rotavirus sa11 within infected cells by ultrastructural immunocytochemistry. monospecific or monoclonal antibodies to selected structural and nonstructural proteins were the primary antisera. the major outer capsid glycoprotein, vp7, was associated with nonenveloped particles, with particles de-enveloped by triton x-100 and with both nuclear and cytoplasmic inclusions. the protease-sensitive outer capsid protein, vp3 ... | 1984 | 6099654 |
| comparative study of rotavirus strains of bovine and rabbit origin. | newborn calves were susceptible to infection with a rotavirus strain isolated from rabbits and, conversely, rabbits 4-5 weeks of age became ill and some died when inoculated with the rotavirus strain of bovine origin. however, the latter isolate appeared to be more virulent than the rabbit isolate in either calves or rabbits. moreover, in reciprocal cross protection tests, carried out in calves and in rabbits, the rabbit rotavirus antiserum afforded only weak protection to challenge infection wi ... | 1984 | 6099786 |
| [seroepidemiology of rotavirus in a population of children, goiânia, goiás, brazil]. | 1984 | 6099909 | |
| role of rotavirus as the cause of acute paediatric diarrhoea in calcutta. | of the 245 acute paediatric diarrhoea cases admitted to the infectious diseases hospital, calcutta between july, 1979 and june, 1981, rotavirus was detected in the faeces of 55 (22.4%) patients either as the sole aetiological agent or in association with other enteropathogens. children aged six months to two years were most commonly infected. the virus was detected throughout the year with higher incidence during the winter months of both years. the frequency of detection of major enteropathogen ... | 1984 | 6099923 |
| the molecular biology of rotaviruses. vii. detailed structural analysis of gene 10 of bovine rotavirus. | cdna cloning and nucleotide sequence analysis have allowed detailed structural studies on rna segment 10 of the u.k. bovine rotavirus to be undertaken. the complete sequence of 751 nucleotides was determined and found to contain only a single long open reading frame capable of coding for a protein of 175 amino acids. the gene has an unusually long 3' untranslated region of 184 nucleotides or some 24.5% of the total sequence, whose start was confirmed by analysing the carboxyterminal amino acid o ... | 1984 | 6099939 |
| isolation and propagation of bovine rotavirus in cell culture. | 1984 | 6100726 | |
| comparison of the polypeptides of bovine rotavirus serotypes. | 1984 | 6100727 | |
| effect of s-adenosylmethionine on human rotavirus rna synthesis. | the characteristics of human rotavirus-associated rna polymerase activity have been examined in relation to the effects of ribonucleoside triphosphate analogs and s-adenosylmethionine. these effects were analyzed by testing two forms of activated virus particles: edta- and heat-treated virions. the former lack outer shell proteins, and activation by means of heat treatment does not introduce any apparent modification in virion structure. virus-associated rna polymerase shows similar properties i ... | 1984 | 6090696 |
| infectious diarrhea of infant rats produced by a rotavirus-like agent. | during the investigation of an outbreak of diarrhea in suckling rats, a virus morphologically identical to but antigenically distinct from rotaviruses was identified. the disease was characterized clinically by erythema and cracking and bleeding of the perianal skin associated with the excretion of poorly formed fecal pellets, liquid, and gas. light microscopy-observable changes consisted of small intestinal villous atrophy, villous epithelial necrosis, and villous epithelial syncytial cell form ... | 1984 | 6090714 |
| isolation of human rotaviruses with a distinct rna electrophoretic pattern from indonesia. | 1984 | 6090872 | |
| [experimental rotavirus infection in a newborn calf]. | 1984 | 6091208 | |
| inactivation of simian rotavirus sa11 by chlorine, chlorine dioxide, and monochloramine. | the kinetics of inactivation of simian rotavirus sa11 by chlorine, chlorine dioxide, and monochloramine were studied at 5 degrees c with a purified preparation of single virions and a preparation of cell-associated virions. inactivation of the virus preparations with chlorine and chlorine dioxide was studied at ph 6 and 10. the monochloramine studies were done at ph 8. with 0.5 mg of chlorine per liter at ph 6, more than 4 logs (99.99%) of the single virions were inactivated in less than 15 s. b ... | 1984 | 6091546 |
| isolation of enteroviruses from water, suspended solids, and sediments from galveston bay: survival of poliovirus and rotavirus adsorbed to sediments. | the distribution and quantitation of enteroviruses among water, suspended solids, and compact sediments in a polluted estuary are described. samples were collected sequentially from water, suspended solids, fluffy sediments (uppermost layer of bottom sediments), and compact sediment. a total of 103 samples were examined of which 27 (26%) were positive for virus. polioviruses were recovered most often, followed by coxsackie b viruses and echoviruses 7 and 29. virus was found most often attached t ... | 1984 | 6091548 |
| micro-organisms in gastroenteritis. | we present bacteriological and virological findings together with salient clinical features from a prospective study of 447 children aged under 2 years admitted to hospital with infectious gastroenteritis. putative pathogenic micro-organisms were identified in the stools of 75% of these children. eight identifiably distinct groups of viruses, found on electron microscopy and tissue culture were present in 67% of patients--rotavirus was detected most frequently. pathogenic bacteria (salmonellas, ... | 1984 | 6091568 |
| [rotavirus and infantile gastroenteritis in new caledonia]. | a two year study, from 1981 to 1982, has been conducted in new caledonia to estimate the importance of rotavirus infections in children with gastroenteritis. on 497 stool specimens, 71 (14.2%) were found positive by using elisa test to demonstrate a rotavirus infection. male children and age group six months-two years appeared to be the most affected. rotavirus infections were more numerous during the dry fresh season (august-november). mixed infections with enteroviruses or enterobacteriae were ... | 1984 | 6091934 |
| viral gastroenteritis. | 1984 | 6092272 | |
| rotavirus-specific antibodies in fetal bovine serum and commercial preparations of serum albumin. | rotavirus-specific antibodies were detected in fetal bovine serum, bovine serum albumin, and human serum albumin by radioimmunoprecipitation with the ncdv strain of bovine rotavirus as the detecting antigen. fetal bovine sera neutralized bovine rotavirus in a plaque reduction neutralization test to titers of 1:20 or greater. immunoglobulins purified from fetal bovine serum by protein a-agarose affinity chromatography precipitated rotavirus antigens but did not neutralize bovine rotavirus. rotavi ... | 1984 | 6092417 |
| three-year prospective study of intestinal pathogens in madrid, spain. | during the period july 1980 through june 1983, in a general hospital in madrid, the following organisms were detected from 6,970 patients with gastroenteritis: 710 salmonella spp. 506 campylobacter jejuni, 379 shigella spp., 12 yersinia enterocolitica, 1,466 rotavirus, 134 giardia lamblia, and 4 entamoeba histolytica. chloramphenicol showed good activity against most tested strains of salmonella spp., shigella spp., and c. jejuni. the incidence of salmonella spp. and shigella spp. was very marke ... | 1984 | 6092418 |
| bovine rotavirus serotypes and their significance for immunization. | neutralization assays on calf fecal rotavirus with antisera to two previously described bovine rotavirus serotypes allowed the isolation of four rotaviruses belonging to a distinct third serotype. in a survey of 85 calf isolates, 80 rotaviruses belonged to serotype 1 (91%), 1 belonged to serotype 2 (1%), and 4 belonged to serotype 3 (5%). serotypes 1 and 2 were shown to not cross-protect in a passive immunization experiment in gnotobiotic lambs. ingestion of specific antiserum protected against ... | 1984 | 6092421 |
| radioimmunofocus assay for detection and quantitation of human rotavirus. | a radioimmunofocus assay suitable for quantitation of cell culture-adapted human rotavirus was developed. the method was reproducible, more sensitive than plaque assay, and useful to detect and quantify strains of rotavirus which do not produce plaques. preliminary results also suggested that the technique will be a useful means of serotyping cell culture-adapted strains of the virus. | 1984 | 6092422 |
| importance of enteric adenoviruses 40 and 41 in acute gastroenteritis in infants and young children. | in a prospective 1-year study of acute infantile gastroenteritis, adenoviruses were detected in the stools or by seroconversions, or both, in 56 of 416 (13.5%) ill children. by use of dna restriction enzyme analysis, enzyme immunoassay, and culture techniques, 33 of 56 (59%) adenovirus specimens were identified as enteric adenoviruses 40 and 41 (ad40 and ad41). they were found as the sole recognizable cause of diarrhea in 30 of 416 (7.2%) ill children and in 0 of 200 controls. three additional i ... | 1984 | 6092424 |
| latex immunoassay for rapid detection of rotavirus. | a latex agglutination (la) test was evaluated for the detection of human rotaviruses in stool specimens. both antiserum and immunoglobulin g (igg)-sensitized latex particles were used, with igg-coated beads being more sensitive for human rotavirus antigen detection. latex beads sensitized with anti-simian-sa-11 igg were stable for at least 8 months when stored at 4 degrees c. the sensitivity of the test was compared with that of the rotazyme (abbott laboratories, diagnostics div., north chicago, ... | 1984 | 6092427 |
| changes in antibody avidity after virus infections: detection by an immunosorbent assay in which a mild protein-denaturing agent is employed. | in titrating serum immunoglobulin g antibody to viruses by enzyme-linked immunosorbent assay, we used two rows of wells for serial twofold dilutions of the serum; in one row, a low concentration of a protein denaturant, 0.5 or 1.0 m guanidine hydrochloride, was added to the diluent so that the binding of low-avidity antibodies to viral antigens on the solid phase was inhibited. we then compared the antibody titration curves obtained in the two rows. we found that the addition of the reagent resu ... | 1984 | 6092430 |
| the use of beta-lactamase in enzyme immunoassays for detection of microbial antigens. | the sensitivity and performance characteristics of enzyme immunoassays (eia) depend to a great extent on the kinetics of the enzyme-substrate system used as indicator. we labeled a variety of polyclonal and monoclonal immunoglobulins with purified beta-lactamase and used them in sensitive eia systems for the detection of a number of microbial antigens. polyclonal antibodies to rotavirus, adenovirus, and haemophilus influenzae type b polyribitol phosphate and monoclonal antibodies to dengue virus ... | 1984 | 6092474 |
| production of subgroup-specific monoclonal antibodies against human rotaviruses and their application to an enzyme-linked immunosorbent assay for subgroup determination. | nonneutralizing monoclonal antibodies were prepared against two strains, s2 and yo, of human rotaviruses isolated in cell culture. s2-37 and yo-5 antibodies had subgroup i and subgroup ii specificities, respectively. the remaining antibodies (s2-65, yo-71, yo-89, and yo-156) reacted commonly with all the rotaviruses examined. all of the monoclonal antibodies agglutinated exclusively single-shelled particles and immunoprecipitated 42,000-dalton protein, a major component of inner capsid. using th ... | 1984 | 6092528 |
| rotavirus shedding by newborn children. | we studied the shedding of rotavirus by newborn children in the nurseries of a large maternity hospital in caracas, venezuela, throughout the year 1982. sixty-two (57%) of 108 children examined shed the virus within the first few days of life. four (6%) of the 62 children who shed rotavirus had diarrhea but only one of them required oral rehydration therapy. the rotavirus specimens were identified as subgroup 2 in an elisa subgrouping assay that employs monoclonal antibodies. analysis of the rna ... | 1984 | 6092529 |
| [comparative studies on the rotavirus syndrome following infection with human rotavirus of the subgroup 1 or 2]. | human rotavirus infection which heals spontaneously causes gastroenteritis in newborns and infants. 150 pediatric patients infected with rotavirus as diagnosed by elisa suffered from diarrhoea for an average of 3 days, from vomiting for 1 day, and/or fever for 1-2 days. nowadays this disease is known as "human rotavirus syndrome". human rotaviruses can be divided into at least 4 serotype antigens and some 3 further subgroup antigens. the serotype antigens are only detectable biologically (e.g. b ... | 1984 | 6092948 |
| [detection of rotavirus in patients with malabsorption]. | investigations were carried out to evaluate frequency and pathogenic effects of rotaviruses. for this purpose 53 biopsy specimens were taken from the jejunum, especially of children at an age up to 15. because of their malabsorption the patients have undergone a special gastroenterological treatment for years or they only recently began with it, being suspected of suffering from this disease. rotavirus antigen was indicated in 43% of the cases by means of immunohistology, at which only less part ... | 1984 | 6092960 |
| the commonest rotaviruses from neonatal lamb diarrhoea in england and wales have atypical electropherotypes. | 1984 | 6093328 | |
| cloning of bovine rotavirus (rf strain): nucleotide sequence of the gene coding for the major capsid protein. | the genes of the rf strain of bovine rotavirus have been cloned into pbr 322 following the synthesis and hybridization of cdna transcribed from both strands of in vitro polyadenylated genomic rna. cloned rotavirus dnas were assigned to most of the 11 genomic rna segments by northern blot hybridization. the complete sequence of gene 6 that codes for the major inner capsid protein has been determined. the gene is 1356 nucleotides long and possesses an unique long open reading frame that could enco ... | 1984 | 6093360 |
| comparison of a commercial enzyme-linked immunosorbent assay with electron microscopy, fluorescent antibody, and virus isolation for the detection of bovine and porcine rotavirus. | the purpose in this study was to compare the sensitivity of a commercial enzyme-linked immunosorbent assay (elisa) with electron microscopy (em), fluorescent antibody (fa), and virus isolation (vi) for the detection of bovine and porcine rotavirus (rv). seventy-three bovine and 116 porcine accessions were evaluated by 1 or all 4 diagnostic tests, where suitable specimens were available. for the bovine samples, agreement was 33% between fa and em, 33% between fa and elisa, and 92% between em and ... | 1984 | 6093642 |
| isolation of murine rotavirus in cell cultures. brief report. | murine rotavirus was isolated in primary monkey kidney cells. electrophoretic pattern of genome rna of murine rotavirus was different from that of human rotaviruses. oral administration of cultured murine rotavirus caused mild diarrhea in newborn balb/c mice. | 1984 | 6093743 |
| false positive rotazyme results. | 1984 | 6141413 | |
| comparison of indirect double antibody and double antibody sandwich elisa techniques with latex agglutination test for the diagnosis of human rotavirus infection. | 94 faecal samples from infants and children suffering of acute gastroenteritis were investigated for rotavirus by indirect double antibody sandwich elisa kit (who, geneva), rotavirus elisa kit (dakopatts a/s, copenhagen) and rotalex latex-agglutination kit (orion diagnostica, helsinki). the elisa techniques gave almost identical results and seemed to be of same sensitivity and specificity. rotalex agglutination had an overall agreement of 88% with elisa. it is concluded that strongly positive re ... | 1984 | 6143499 |
| protection of infants against rotavirus diarrhoea by rit 4237 attenuated bovine rotavirus strain vaccine. | a randomised, double-blind, placebo-controlled trial was conducted to evaluate the ability of rit 4237 live attenuated bovine rotavirus (subgroup 1) vaccine strain to protect against natural rotavirus infection in children. 178 infants aged 8 to 11 months received a single oral dose of rit 4237 vaccine or placebo and were followed up serologically and clinically during a subgroup 2 rotavirus epidemic. no side-effects attributable to the vaccine were observed. during the 5 months' observation aft ... | 1984 | 6143964 |
| an enveloped virus in stools of children and adults with gastroenteritis that resembles the breda virus of calves. | pleomorphic virus-like particles about 100 nm in diameter with a fringe of closely applied peplomers (7-9 nm in length) were observed by electron microscopy in the stools of 20 children and adults with gastroenteritis. in most of the samples no other viral or bacterial pathogens were detected. in form and under immune electron microscopy these virus-like particles resembled the breda virus isolated from diarrhoeic calves. these objects may be a viral pathogen of humans. | 1984 | 6143978 |
| waterborne outbreak of rotavirus diarrhoea in adults in china caused by a novel rotavirus. | during two epidemics of acute diarrhoea in china in late 1982/early 1983, more than 12 000 adults in two coal mining districts were affected. the virus isolated from stool samples resembled a rotavirus morphologically. antigenically it lacked the group antigen shared by known rotaviruses. like other rotaviruses it had a double-stranded rna with 11 discrete segments, but the pattern of migration of the segments on polyacrylamide electrophoresis differed from those of other rotaviruses. | 1984 | 6144874 |
| increased "take" rate of oral rotavirus vaccine in infants after milk feeding. | 1984 | 6147727 | |
| electron microscopic investigations of rotavirus morphogenesis in cell cultures. | the replication of simian rotavirus sa11 in gmk cells and of bovine rotavirus in calf kidney cells was studied by electron microscopy. by 30 min post-inoculation (p.i.) sa11 virus was absorbed to the cell membrane in the absence of trypsin and became engulfed into the cell; clusters of viral particles were internalized also into cytoplasmic vacuoles. at 2 hr p.i., viral particles were seen in lysosomes and 6 hr p.i., the first progeny virus was found in the cisternae of endoplasmic reticulum (er ... | 1984 | 6147993 |
| planar lattices in faeces of babies. | 1984 | 6150267 |