Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| components of arabidopsis defense- and ethylene-signaling pathways regulate susceptibility to cauliflower mosaic virus by restricting long-distance movement. | we analyzed the susceptibility of arabidopsis mutants with defects in salicylic acid (sa) and jasmonic acid (ja)/ethylene (et) signaling to infection by cauliflower mosaic virus (camv). mutants cpr1-1 and cpr5-2, in which sa-dependent defense signaling is activated constitutively, were substantially more resistant than the wild type to systemic infection, implicating sa signaling in defense against camv. however, sa-deficient nahg, sid2-2, eds5-1, and pad4-1 did not show enhanced susceptibility. ... | 2007 | 17555274 |
| phosphorylation of the termini of cauliflower mosaic virus precapsid protein is important for productive infection. | cauliflower mosaic virus (camv) coat protein precursor (pre-cp) has 489 amino acids (p57) and is processed by the viral proteinase into three major forms: p44, p39, and p37. the n- and c-terminal extensions of pre-cp are released during maturation by the virus-encoded proteinase. we showed that these extensions are phosphorylated at several sites by host casein kinase ii (ckii). we have identified the phosphorylated amino acids using an in vitro phosphorylation assay and tested the effect of mut ... | 2007 | 17555273 |
| isolation of two highly active soybean (glycine max (l.) merr.) promoters and their characterization using a new automated image collection and analysis system. | a novel automated image collection and analysis system was used to compare two new soybean (glycine max (l.) merr.) promoters with the cauliflower mosaic virus 35s (camv35s) promoter, which was used as an expression standard. for expression comparisons, various permutations of a soybean polyubiquitin (gmubi) promoter, a soybean heat shock protein 90-like (gmhsp90l) promoter and the camv35s promoter were placed upstream of a green fluorescent protein (gfp) gene. dna constructs were introduced via ... | 2007 | 17503049 |
| increased endogenous methyl jasmonate altered leaf and root development in transgenic soybean plants. | methyl jasmonate (meja) is a plant-signaling molecule that regulates plant morphogenesis and expression of plant defense genes. to determine the role of the endogenous meja levels in the development of plants, transgenic soybean [glycine max (l.) merrill] plants harboring ntr1 gene encoding for jasmonic acid carboxyl methyltransferase (jmt) were produced. the activation of ntr1 gene expression resulted in the production of meja. overexpression of the ntr1 cdna under the regulation of cauliflower ... | 2007 | 17498632 |
| antisense expression of 3-oxoacyl-acp reductase affects whole plant productivity and causes collateral changes in activity of fatty acid synthase components. | brassica napus cv westar plants were transformed with 3-oxoacyl-acp reductase (kr) in antisense orientation, driven by either the cauliflower mosaic virus 35s promoter or a seed-specific acyl carrier protein promoter to determine the effects on plant productivity and on the activity of other fatty acid synthase (fas) components. in plants with altered kr activity, total seed yield was reduced in all cases. in less severely affected plant lines, seeds had a normal appearance and composition but t ... | 2007 | 17401135 |
| overexpression of homologous phytochrome genes in tomato: exploring the limits in photoperception. | transgenic tomato [lycopersicon esculentum (=solanum lycopersicum)] lines overexpressing tomato phya, phyb1, or phyb2, under control of the constitutive double-35s promoter from cauliflower mosaic virus (camv) have been generated to test the level of saturation in individual phytochrome-signalling pathways in tomato. western blot analysis confirmed the elevated phytochrome protein levels in dark-grown seedlings of the respective phy overexpressing (phyoe) lines. exposure to 4 h of red light resu ... | 2007 | 17251177 |
| electron-lucent inclusion bodies are structures specialized for aphid transmission of cauliflower mosaic virus. | cauliflower mosaic virus (camv) is transmitted by aphids. for acquisition by the vector, a transmissible complex must form, composed of the virus particle, the viral coat-associated protein p3 and the helper protein p2. however, the components of the transmissible complex are largely separated in infected plant cells: most p3 virions are confined in electron-dense inclusion bodies, whereas p2 is sequestered in electron-lucent inclusion bodies (elibs). this spatial separation controls virus acqui ... | 2007 | 17872542 |
| nodulation studies in the model legume medicago truncatula: advantages of using the constitutive ef1alpha promoter and limitations in detecting fluorescent reporter proteins in nodule tissues. | the cauliflower mosaic virus 35s promoter currently is being used in rnai-based approaches for attenuating host gene expression during legume root nodule development and also for the expression of fluorescent reporters in nodule tissues. in this study, we have evaluated the expression of this promoter in the indeterminate nodules of the model plant medicago truncatula. our results clearly show that the 35s promoter is inactive in both the nodule meristem and in bacteroid-containing cells of the ... | 2007 | 17849706 |
| cauliflower mosaic virus protein p6 is a suppressor of rna silencing. | we infected a transgenic arabidopsis line (gxa), containing an amplicon-silenced 35s : : gfp transgene, with cauliflower mosaic virus (camv), a plant pararetrovirus with a dna genome. systemically infected leaves showed strong gfp fluorescence and amplicon transcripts were detectable in northern blots, indicating that silencing of gfp had been suppressed during camv-infection. transgenic arabidopsis lines expressing camv protein p6, the major genetic determinant of symptom severity, were crossed ... | 2007 | 18024914 |
| a protein key to plant virus transmission at the tip of the insect vector stylet. | hundreds of species of plant viruses, many of them economically important, are transmitted by noncirculative vector transmission (acquisition by attachment of virions to vector mouthparts and inoculation by subsequent release), but virus receptors within the vector remain elusive. here we report evidence for the existence, precise location, and chemical nature of the first receptor for a noncirculative virus, cauliflower mosaic virus, in its insect vector. electron microscopy revealed virus-like ... | 2007 | 17962414 |
| the cytochrome p450 enzyme cyp96a15 is the midchain alkane hydroxylase responsible for formation of secondary alcohols and ketones in stem cuticular wax of arabidopsis. | most aerial surfaces of plants are covered by cuticular wax that is synthesized in epidermal cells. the wax mixture on the inflorescence stems of arabidopsis (arabidopsis thaliana) is dominated by alkanes, secondary alcohols, and ketones, all thought to be formed sequentially in the decarbonylation pathway of wax biosynthesis. here, we used a reverse-genetic approach to identify a cytochrome p450 enzyme (cyp96a15) involved in wax biosynthesis and characterized it as a midchain alkane hydroxylase ... | 2007 | 17905869 |
| physiological roles of the light, oxygen, or voltage domains of phototropin 1 and phototropin 2 in arabidopsis. | phototropins (phot1 and phot2) are plant blue-light receptors that mediate phototropism, chloroplast movement, stomatal opening, rapid inhibition of growth of etiolated seedlings, and leaf expansion in arabidopsis (arabidopsis thaliana). their n-terminal region contains two light, oxygen, or voltage (lov) domains, which bind flavin mononucleotide and form a covalent adduct between a conserved cysteine and the flavin mononucleotide chromophore upon photoexcitation. the c-terminal region contains ... | 2007 | 17085510 |
| plant native tryptophan synthase beta 1 gene is a non-antibiotic selection marker for plant transformation. | gene transformation is an integral tool for plant genetic engineering. all antibiotic resistant genes currently employed are of bacterial origin and their presence in the field is undesirable. therefore, we developed a novel and efficient plant native non-antibiotic selection system for the selection of transgenic plants in the model system arabidopsis. this new system is based on the enhanced expression of arabidopsis tryptophan synthase beta 1 (attsb1) and the use of 5-methyl-tryptophan (5mt, ... | 2007 | 17039373 |
| cauliflower mosaic virus orf vii is not required for aphid transmissibility. | 2007 | 3207505 | |
| expression of xylanase with high specific activity from streptomyces olivaceoviridis a1 in transgenic potato plants (solanum tuberosum l.). | the gene, xynb, from streptomyces olivaceoviridis a1 encoding xylanase, xynb, with a high specific activity for xylan, was transformed into potato (solanum tuberosum l.) by agrobacterium tumefaciens. the integration of xynb into genomic dna was confirmed by pcr and reverse transcriptase-pcr. the gene was expressed under the control of a constitutive double cauliflower mosaic virus (camv) 35s promoter. both sds-page and western blot analysis showed high levels of expression of the 21 kda and 31 k ... | 2007 | 17206370 |
| overexpression of the chimeric gene of the floral regulator constans and the ear motif repressor causes late flowering in arabidopsis. | the transcription factor constans (co) plays a central role in the photoperiod pathway by integrating the circadian clock and light signals into a control for flowering time. co induces flowering locus t (ft) and suppressor of overexpression of co 1 (soc1) expression, and thereby promotes flowering. the ethylene-responsive element-binding factor associated amphiphilic repression (ear) motif was used to construct a constans-ear motif repressor gene (co-rep), which was overexpressed in arabidopsis ... | 2007 | 17219103 |
| the leatl6-associated ubiquitin/proteasome system may contribute to fungal elicitor-activated defense response via the jasmonic acid-dependent signaling pathway in tomato. | the expression of leatl6, an ortholog of arabidopsis atl6 that encodes a ring-h2 finger protein, was induced in tomato roots treated with a cell wall protein fraction (cwp) elicitor of the biocontrol agent pythium oligandrum. the leatl6 protein was expressed as a fusion protein with a maltose-binding protein (mbp) in escherichia coli, and it catalyzed the transfer of ubiquitin to the mbp moiety on incubation with ubiquitin, the ubiquitin-activating enzyme e1, and the ubiquitin-conjugating enzyme ... | 2007 | 17249424 |
| a defect in carbohydrate metabolism ameliorates symptom severity in virus-infected arabidopsis thaliana. | altered starch accumulation is a characteristic biochemical symptom of virus infection in plants. to assess its biological importance, infection of arabidopsis thaliana with turnip vein-clearing virus, cucumber mosaic virus or cauliflower mosaic virus was investigated in plants grown under continuous illumination (under which there is no net breakdown of starch) and in pgm1 mutant plants lacking chloroplastic phosphoglucomutase, an enzyme required for starch biosynthesis. virus-infected wild-typ ... | 2007 | 17170466 |
| nitrogen-dependent posttranscriptional regulation of the ammonium transporter atamt1;1. | ammonium transporter (amt) proteins of the amt family mediate the transport of ammonium across plasma membranes. to investigate whether amts are regulated at the posttranscriptional level, a gene construct consisting of the cauliflower mosaic virus 35s promoter driving the arabidopsis (arabidopsis thaliana) amt1;1 gene was introduced into tobacco (nicotiana tabacum). ectopic expression of atamt1;1 in transgenic tobacco lines led to high transcript levels and protein levels at the plasma membrane ... | 2007 | 17172286 |
| resistance to root-knot nematode in tomato roots expressing a nematicidal bacillus thuringiensis crystal protein. | our laboratory has demonstrated previously that bacillus thuringiensis (bt) crystal (cry) proteins present in the cry5 and cry6 subclades intoxicate free-living nematodes. in this study, we tested whether the expression of nematicidal cry6a in transgenic plants provided protection against plant-parasitic nematodes. as bacterial codon usage is incompatible with expression in plants, two different codon-modified cry6a genes were synthesized for expression in plants. one was designed by maintaining ... | 2007 | 17451491 |
| transgenic nicotiana benthamiana plants resistant to cucumber green mottle mosaic virus based on rna silencing. | rna silencing technology was used to confer resistance to cucumber green mottle mosaic virus (cgmmv). nicotiana benthamiana was transformed with a transgene designed to produce an inverted repeat rna containing cgmmv-coat protein gene (cp) sequences, which were separated by an intron sequence, under the control of the cauliflower mosaic virus 35s promoter. we attempted to confirm the resistance of seven independent transgenic lines; five lines showed resistance to cgmmv infection. the systemic s ... | 2007 | 17453214 |
| isolation and functional analysis of a myb transcription factor gene that is a key regulator for the development of red coloration in apple skin. | red coloration of apple (malus x domestica) skin is an important determinant of consumer preference and marketability. anthocyanins are responsible for this coloration, and their accumulation is positively correlated with the expression level of anthocyanin biosynthetic genes. regulation of expression of these genes is believed to be controlled by myb transcription factors, and the myb transcription factors involved in the activation of anthocyanin biosynthetic genes have been isolated in variou ... | 2007 | 17526919 |
| attenuation of the hypersensitive response by an atpase associated with various cellular activities (aaa) protein through suppression of a small gtpase, adp ribosylation factor, in tobacco plants. | atpase associated with various cellular activities (aaa) proteins are commonly distributed among eukaryotes, and are involved in a multitude of cellular functions. ntaaa1 is one such example, being involved in pathogen response in tobacco plants. when its activity was suppressed in rnai transgenic tobacco plants, an elevated resistance to the pathogenic bacterium pseudomonas syringae was observed in comparison with the wild type. as aaa proteins function through interaction with specific partner ... | 2007 | 17559512 |
| expression of bacillus subtilis proba genes and reduction of feedback inhibition of proline synthesis increases proline production and confers osmotolerance in transgenic arabidopsis. | proline accumulation has been shown to correlate with tolerance to drought and salt stresses in plants. we attempt to introduce the wild-type, mutant, and fusion proba genes derived from bacillus subtilis into arabidopsis thaliana under the control of a strong promoter cauliflower mosaic virus 35s (camv35s). the transgenic plants produced higher level of free proline than control and the overproduction of proline resulted in the increased tolerance to osmotic stress in transgenic plants. besides ... | 2007 | 17562291 |
| expression of a recombinant human sperm-agglutinating mini-antibody in tobacco (nicotiana tabacum l.). | the murine monoclonal antibody (mab) s19 recognizes an n-linked carbohydrate antigen designated sperm agglutination antigen-1 (saga1) located on the membrane protein cd52. this antigen is added to the sperm surface during epididymal maturation. binding of the s19 mab to saga-1 causes the rapid agglutination of sperm and blocks pre-fertilization events. previous studies indicated that the s19 mab may be a potential specific spermicidal agent (termed a spermistatic) capable of replacing current sp ... | 2007 | 17566292 |
| a rice promoter containing both novel positive and negative cis-elements for regulation of green tissue-specific gene expression in transgenic plants. | the tissue-specific expression of transgenes is essential in plant breeding programmes to avoid the fitness costs caused by constitutive expression of a target gene. however, knowledge on the molecular mechanisms of tissue-specific gene expression and practicable tissue-specific promoters is limited. in this study, we identified the cis-acting elements of a tissue-specific promoter from rice, p(d54o), and tested the application of original and modified p(d54o) and its cis-elements in the regulat ... | 2007 | 17596180 |
| indicated detection of two unapproved transgenic rice lines contaminating vermicelli products. | we analyzed the dna fragments extracted from four rice vermicelli products. the bacillus thuringiensis (bt) rice line, which has a construct similar to the gm shanyou 63 line, was detected in some vermicelli products by identification of the junction region sequence between rice act1 promoter and the cry1ac gene, and that between cry1ac and nos. in addition, we also detected a different bt rice line by means of the junction region sequence between the maize ubiquitin promoter and cry1ab gene and ... | 2007 | 17608495 |
| functional analysis of cauliflower mosaic virus 35s promoter: re-evaluation of the role of subdomains b5, b4 and b2 in promoter activity. | the cauliflower mosaic virus 35s (35s) promoter is used extensively for transgene expression in plants. the promoter has been delineated into different subdomains based on deletion analysis and gain-of-function studies. however, cis-elements important for promoter activity have been identified only in the domains b1 (as-2 element), a1 (as-1 element) and minimal promoter (tata box). no cis-elements have been described in subdomains b2-b5, although these are reported to be important for the overal ... | 2007 | 17608668 |
| enhanced resistance and neutralization of defense responses by suppressors of rna silencing. | the effects of transgenic expression of the potato virus y (pvy) hcpro silencing suppressor in tobacco were examined on infection by several viruses. infection by tobacco mosaic virus (tmv) was reduced at 25 degrees c, but not at 33 degrees c. by contrast, systemic infection at 33 degrees c by the tmv expressing green fluorescent protein was promoted by the hcpro. infection by tobacco rattle virus (trv) was restricted to local necrotic lesions by the pvy hcpro. however, this resistance was neutr ... | 2007 | 17617488 |
| heat suppresses activation of an auxin-responsive promoter in cultured guard cell protoplasts of tree tobacco. | cultured guard cell protoplasts (gcp) of tree tobacco (nicotiana glauca) comprise a novel system for investigating the cell signaling mechanisms that lead to acquired thermotolerance and thermoinhibition. at 32 degrees c in a medium containing an auxin (1-naphthaleneacetic acid [naa]) and a cytokinin (6-benzylaminopurine), gcp expand, regenerate cell walls, dedifferentiate, and divide. at 38 degrees c, gcp acquire thermotolerance within 24 h, but their expansion is limited and they neither regen ... | 2007 | 17704234 |
| over-expression of the arabidopsis dre/crt-binding transcription factor dreb2c enhances thermotolerance. | the dehydration responsive element binding protein 2 (dreb2) subgroup belongs to the plant-specific apetala2/ethylene-responsive element binding factor (ap2/erf) family of transcription factors. we have characterized cdna encoding arabidopsis thaliana dreb2c, which is induced by mild heat stress. both an electrophoretic mobility shift assay (emsa) and a yeast one-hybrid assay revealed that dreb2c(145-528) was able to form a complex with the dehydration responsive element/c-repeat (dre/crt; a/gcc ... | 2007 | 17716623 |
| expression of the membrane-associated resistance protein rpw8 enhances basal defense against biotrophic pathogens. | the powdery mildew resistance genes rpw8.1 and rpw8.2 from arabidopsis differ from the other isolated plant resistance (r) genes in their predicted protein domains and their resistance spectrum. the two homologous rpw8 genes encode small proteins featuring a predicted amino-terminal transmembrane anchor domain and a coiled-coil domain and confer resistance to a broad spectrum of powdery mildews. here, we show that arabidopsis plants expressing the rpw8 genes have enhanced resistance to another b ... | 2007 | 17722700 |
| high-efficiency protein expression in plants from agroinfection-compatible tobacco mosaic virus expression vectors. | plants are increasingly being examined as alternative recombinant protein expression systems. recombinant protein expression levels in plants from tobacco mosaic virus (tmv)-based vectors are much higher than those possible from plant promoters. however the common tmv expression vectors are costly, and at times technically challenging, to work with. therefore it was a goal to develop tmv expression vectors that express high levels of recombinant protein and are easier, more reliable, and more co ... | 2007 | 17723150 |
| iron acquisition by phytosiderophores contributes to cadmium tolerance. | based on the ability of phytosiderophores to chelate other heavy metals besides iron (fe), phytosiderophores were suggested to prevent graminaceous plants from cadmium (cd) toxicity. to assess interactions between cd and phytosiderophore-mediated fe acquisition, maize (zea mays) plants were grown hydroponically under limiting fe supply. exposure to cd decreased uptake rates of 59fe(iii)-phytosiderophores and enhanced the expression of the fe-phytosiderophore transporter gene zmys1 in roots as we ... | 2007 | 17337530 |
| the cauliflower mosaic virus (camv) 35s promoter sequence alters the level and patterns of activity of adjacent tissue- and organ-specific gene promoters. | here we report the effect of the 35s promoter sequence on activities of the tissue- and organ-specific gene promoters in tobacco plants. in the absence of the 35s promoter sequence the aap2 promoter is active only in vascular tissues as indicated by expression of the aap2:gus gene. with the 35s promoter sequence in the same t-plasmid, transgenic plants exhibit twofold to fivefold increase in aap2 promoter activity and the promoter becomes active in all tissue types. transgenic plants hosting the ... | 2007 | 17340093 |
| expression of a heat stable phytase from aspergillus fumigatus in tobacco (nicotiana tabacum l. cv. nc89). | aspergillus fumigatus contains a heat-stable phytase of great potential. to determine whether this phytase could be expressed in plants as a functional enzyme, we introduced the phytase gene from a. fumigatus (fphya) in tobacco (nicotiana tabacum l. cv. nc89) by agrobacterium-mediated transformation. phytase expression was controlled by the cauliflower mosaic virus (camv) 35s promoter. secretion of recombinant phytase (tfphya) to the extracellular fluid was established by use of the signal seque ... | 2007 | 17385337 |
| improved expression of recombinant gfp using a replicating vector based on beet curly top virus in leaf-disks and infiltrated nicotiana benthamiana leaves. | recombinant green fluorescent protein (gfp) with a molecular mass of 29 kda was transiently expressed in agrobacterium-inoculated leaf-disks prepared from nicotiana benthamiana plants. expression of gfp from the cauliflower mosaic virus (camv) 35 s promoter within a replicating vector based on the geminivirus beet curly top virus (bctv) was more than 3 times higher than from a control, non-replicating vector. use of the cassava vein mosaic virus (csvmv) promoter in the bctv replicating vector in ... | 2007 | 17294255 |
| a monopartite begomovirus-associated dna beta satellite substitutes for the dna b of a bipartite begomovirus to permit systemic infection. | dna beta is a circular single-stranded satellite dna which co-infects with certain monopartite helper begomoviruses to cause economically important diseases, such as cotton leaf curl disease (clcud). dna beta encodes a single protein, betac1. tomato leaf curl new delhi virus (tolcndv) is a bipartite begomovirus in which both dna a and dna b are required for systemic infection. inoculation of tomato plants with tolcndv dna a alone induced local but not systemic infection, whereas co-inoculation w ... | 2007 | 17872543 |
| [effective expression of the gene encoding an extracellular ribonuclease of zinnia elegans in the sr1 nicotiana tabacum plants]. | complementary dna for the extracellular rnase of zinnia elegans was cloned under control of the cauliflower mosaic virus 35s rna constitutive promoter and transferred into the nicotiana tabacum sr1 plants. primary tobacco transformants were characterized by a high level of rnase activity. | 2007 | 17899821 |
| novel plant transformation vectors containing the superpromoter. | we developed novel plasmids and t-dna binary vectors that incorporate a modified and more useful form of the superpromoter. the superpromoter consists of a trimer of the octopine synthase transcriptional activating element affixed to the mannopine synthase2' (mas2') transcriptional activating element plus minimal promoter. we tested a superpromoter-beta-glucuronidasea fusion gene in stably transformed tobacco (nicotiana tabacum) and maize (zea mays) plants and in transiently transformed maize bl ... | 2007 | 17932307 |
| improving zinc content and antioxidant activity in transgenic tomato plants with expression of mouse metallothionein-i by mt-i gene. | metallothioneins (mts), as a family of low-molecular-weight, cysteine-rich, and metal-binding proteins, show potential for utilization in functional food. tomato plants were transformed with gene constructs that contained mt-i encoding the mouse mt-i, similar in sense orientation with the constitutively active double 35s promoter from cauliflower mosaic virus. three independent transformants, which had copies of the gene in their genomes, were obtained. in these transgenic lines, high-level expr ... | 2007 | 17960876 |
| chloroplast-like organelles were found in enucleate sieve elements of transgenic plants overexpressing a proteinase inhibitor. | sapin2a, a plant proteinase inhibitor from nightshade (solanum americanum), was located to the enucleate sieve elements (ses) of phloem. the expressed sapin2a in transgenic lettuce showed inhibition of plant endogenous trypsin- and chymotrypsin-like activities, suggesting that sapin2a can regulate proteolysis in plant cells. to further investigate the physiological role of sapin2a, we produced transgenic nightshade and lettuce plants overexpressing sapin2a from the cauliflower mosaic virus (camv ... | 2007 | 17986772 |
| overexpression of the apple mpnpr1 gene confers increased disease resistance in malus x domestica. | the npr1 gene plays a pivotal role in systemic acquired resistance in plants. its overexpression in arabidopsis and rice results in increased disease resistance and elevated expression of pathogenesis-related (pr) genes. an npr1 homolog, mpnpr1-1, was cloned from apple (malus x domestica) and overexpressed in two important apple cultivars, galaxy and m26. apple leaf pieces were transformed with the mpnpr1 cdna under the control of the inducible pin2 or constitutive cauliflower mosaic virus (camv ... | 2007 | 17990964 |
| the hypersensitive response to tomato leaf curl new delhi virus nuclear shuttle protein is inhibited by transcriptional activator protein. | the hypersensitive response (hr) is a common feature of plant disease resistance reactions and a type of programmed cell death (pcd). many pathogens are able to modulate pathways involved in cell death. in contrast to animal viruses, inhibitors of pcd activity have not been identified for plant-infecting viruses. previously, we have reported that the nuclear shuttle protein (nsp) of tomato leaf curl new delhi virus (tolcndv) induces an hr in nicotiana tabacum and lycopersicon esculentum plants w ... | 2007 | 17990965 |
| expression of a beta-glucosidase gene results in increased accumulation of salicylic acid in transgenic nicotiana tabacum cv. xanthi-nc nn genotype. | a beta-glucosidase gene (bgla) from butyrivibrio fibrisolvens h17c was cloned into the binary vector pga482 under the control of the 35s cauliflower mosaic virus (camv) promoter. a second construct was generated for accumulation of the bgla gene product in the vacuole of transformed tobacco plants. reverse transcription-polymerase chain reaction analysis demonstrated that the bgla gene was expressed in 71% of cytosol-targeted and 67% of vacuole-targeted transgenic tobacco t(1) plants. t(1) trans ... | 2007 | 17082925 |
| expression and characterization of acidothermus cellulolyticus e1 endoglucanase in transgenic duckweed lemna minor 8627. | endoglucanase e1 from acidothermus cellulolyticus was expressed cytosolically under control of the cauliflower mosaic virus 35s promoter in transgenic duckweed, lemna minor 8627 without any obvious observable phenotypic effects on morphology or rate of growth. the recombinant enzyme co-migrated with the purified catalytic domain fraction of the native e1 protein on western blot analysis, revealing that the cellulose-binding domain was cleaved near or in the linker region. the duckweed-expressed ... | 2007 | 17127051 |
| enhanced cd2+ -selective root-tonoplast-transport in tobaccos expressing arabidopsis cation exchangers. | several arabidopsis cation exchangers (caxs) encode tonoplast-localized transporters that appear to be major contributors to vacuolar accumulation/sequestration of cadmium (cd(2+)), an undesirable pollutant ion that occurs in man largely as a result of dietary consumption of aerial tissues of food plants. but, ion-selectivity of individual cax transporter types remains largely unknown. here, we transformed nicotiana tabacum with several cax genes driven by the cauliflower mosaic virus (camv) 35s ... | 2007 | 16845524 |
| characterization of a novel plant promoter specifically induced by heavy metal and identification of the promoter regions conferring heavy metal responsiveness. | the bean (phaseolus vulgaris) stress-related gene number 2 (pvsr2) gene responds to heavy metals but not to other forms of environmental stresses. to elucidate its heavy metal-regulatory mechanism at the transcriptional level, we isolated and characterized the promoter region (-1623/+48) of pvsr2. deletions from the 5' end revealed that a sequence between -222 and -147 relative to the transcriptional start site was sufficient for heavy metal-specific induction of the promoter region of pvsr2. de ... | 2007 | 16861574 |
| alteration of organic acid metabolism in arabidopsis overexpressing the maize c4 nadp-malic enzyme causes accelerated senescence during extended darkness. | the full-length cdna encoding the maize (zea mays) c(4) nadp-malic enzyme was expressed in arabidopsis (arabidopsis thaliana) under the control of the cauliflower mosaic virus 35s promoter. homozygous transgenic plants (mem) were isolated with activities ranging from 6- to 33-fold of those found in the wild type. the transformants did not show any differences in morphology and development when grown in long days; however, dark-induced senescence progressed more rapidly in mem plants compared to ... | 2007 | 17885087 |
| subcellular localization and functional domain studies of defective kernel1 in maize and arabidopsis suggest a model for aleurone cell fate specification involving crinkly4 and supernumerary aleurone layer1. | defective kernel1 (dek1), which consists of a membrane-spanning region (dek1-mem) and a calpain-like cys proteinase region (dek1-calp), is essential for aleurone cell formation at the surface of maize (zea mays) endosperm. immunolocalization and fm4-64 dye incubation experiments showed that dek1 and crinkly4 (cr4), a receptor kinase implicated in aleurone cell fate specification, colocalized to plasma membrane and endosomes. supernumerary aleurone layer1 (sal1), a negative regulator of aleurone ... | 2007 | 17933905 |
| posttranscriptional regulation of high-affinity sulfate transporters in arabidopsis by sulfur nutrition. | high-affinity sulfate transporters sultr1;1 and sultr1;2 are expressed at epidermis and cortex of arabidopsis (arabidopsis thaliana) roots during sulfur limitation. here, we report that sultr1;1 and sultr1;2 are two essential components of the sulfate uptake system in roots and are regulated at posttranscriptional levels together with the previously reported transcriptional control. double knockout of sultr1;1 and sultr1;2 by t-dna insertion gene disruption resulted in complete lack of sulfate u ... | 2007 | 17720755 |
| tagging of mads domain proteins for chromatin immunoprecipitation. | most transcription factors fulfill their role in complexes and regulate their target genes upon binding to dna motifs located in upstream regions or introns. to date, knowledge about transcription factor target genes and their corresponding transcription factor binding sites are still very limited. two related methods that allow in vivo identification of transcription factor binding sites are chromatin immunoprecipitation (chip) and chromatin affinity purification (chap). for chap, the protein o ... | 2007 | 17868439 |
| the gigantea-regulated microrna172 mediates photoperiodic flowering independent of constans in arabidopsis. | regulated rna metabolism appears to be a critical component of molecular mechanisms directing flowering initiation in plants. a group of rna binding proteins exerts their roles through the autonomous flowering pathway. posttranscriptional mechanisms regulated by micrornas (mirnas) also play a key role in flowering-time control. here, we demonstrate that the gigantea (gi)-regulated mir172 defines a unique genetic pathway that regulates photoperiodic flowering by inducing flowering locus t (ft) in ... | 2007 | 17890372 |
| a putative hydroxysteroid dehydrogenase involved in regulating plant growth and development. | we have functionally characterized an arabidopsis (arabidopsis thaliana) gene athsd1 (at5g50600) that encodes a protein with homology to animal 11-beta-hydroxysteroid dehydrogenase (hsd). transgenic arabidopsis plants overexpressing athsd1 (designated aohsd plants) under the control of the cauliflower mosaic virus 35s promoter showed increased growth and seed yield as well as increased tolerance of saline stress and reduced seed dormancy. in canola (brassica napus), transgenic plants overexpress ... | 2007 | 17616511 |
| roles of arabidopsis cyclin-dependent kinase c complexes in cauliflower mosaic virus infection, plant growth, and development. | the c-terminal domain (ctd) of rna polymerase ii is phosphorylated during the transcription cycle by three cyclin-dependent kinases (cdks): cdk7, cdk8, and cdk9. cdk9 and its interacting cyclin t partners belong to the positive transcription elongation factor b (p-tefb) complexes, which phosphorylate the ctd to promote transcription elongation. we report that arabidopsis thaliana cdk9-like proteins, cdkc;1 and cdkc;2, and their interacting cyclin t partners, cyct1;4 and cyct1;5, play important r ... | 2007 | 17468259 |
| the transcription factor idef1 regulates the response to and tolerance of iron deficiency in plants. | iron is essential for most living organisms and is often the major limiting nutrient for normal growth. plants induce iron utilization systems under conditions of low iron availability, but the molecular mechanisms of gene regulation under iron deficiency remain largely unknown. we identified the rice transcription factor idef1, which specifically binds the iron deficiency-responsive cis-acting element ide1. idef1 belongs to an uncharacterized branch of the plant-specific transcription factor fa ... | 2007 | 18025467 |
| over-expression of udp-glucose pyrophosphorylase in hybrid poplar affects carbon allocation. | the effects of the over-expression of the acetobacter xylinum udp-glucose pyrophosphorylase (ugpase) under the control of the tandem repeat cauliflower mosaic virus promoter (2x35s) on plant metabolism and growth were investigated in hybrid poplar (populus albaxgrandidentata). transcript levels, enzyme activity, growth parameters, leaf morphology, structural and soluble carbohydrates, and soluble metabolite levels were quantified in both transgenic and wild-type trees. transgenic 2x35s::ugpase p ... | 2007 | 18182429 |
| heterologous acidothermus cellulolyticus 1,4-beta-endoglucanase e1 produced within the corn biomass converts corn stover into glucose. | commercial conversion of lignocellulosic biomass to fermentable sugars requires inexpensive bulk production of biologically active cellulase enzymes, which might be achieved through direct production of these enzymes within the biomass crops. transgenic corn plants containing the catalytic domain of acidothermus cellulolyticus e1 endo-1,4-beta glucanase and the bar bialaphos resistance coding sequences were generated after biolistic (biorad hercules, ca) bombardment of immature embryo-derived ce ... | 2007 | 18478390 |
| a new strategy to improve a cauliflower mosaic virus vector. | co-infection of plants with non-overlapping deletion mutants of cauliflower mosaic virus usually leads to the production of the wild-type virus. to prevent this, a pair of mutants with overlapping deletions was constructed. in infected plants both mutant dnas were stably maintained. such mutants with overlapping deletions will be used as a vector to overcome the size limitation of genes to be cloned. | 2007 | 1937020 |
| point mutations in cauliflower mosaic virus gene vi confer host-specific symptom changes. | mutants of cauliflower mosaic virus (camv) strain d4 have been characterized with regard to host-specific phenotypes that resulted from specific changes in the viral dna sequence. both the mutant and the wild-type viruses infect a brassicaceous host, brassica campestris, systemically, giving indistinguishable symptoms. however, in the solanaceous host datura stramonium, which was systemically infectible by the wild-type virus, mutants induced necrotic local lesions at 21 degrees c and above, and ... | 2007 | 2134858 |
| pathogen phytosensing: plants to report plant pathogens. | real-time systems that provide evidence of pathogen contamination in crops can be an important new line of early defense in agricultural centers. plants possess defense mechanisms to protect against pathogen attack. inducible plant defense is controlled by signal transduction pathways, inducible promoters and cis-regulatory elements corresponding to key genes involved in defense, and pathogen-specific responses. identified inducible promoters and cis-acting elements could be utilized in plant se ... | 2008 | 27879840 |
| hibiscus chlorotic ringspot virus coat protein inhibits trans-acting small interfering rna biogenesis in arabidopsis. | many plant and animal viruses have evolved suppressor proteins to block host rna silencing at various stages of the rna silencing pathways. hibiscus chlorotic ringspot virus (hcrsv) coat protein (cp) is capable of suppressing the transiently expressed sense-rna-induced post-transcriptional gene silencing (ptgs) in nicotiana benthamiana. here, constitutively expressed hcrsv cp from transgenic arabidopsis was found to be able to rescue expression of the silenced gus transgene. the hcrsv cp-transge ... | 2008 | 18753245 |
| proximity to the promoter inhibits recognition of cauliflower mosaic virus polyadenylation signal. | the replication of retroviruses and pararetroviruses (such as caulimo- and hepadnaviruses) involves the production of terminally redundant genomic-length rna. the sequence repeated at both ends of the rna (the r region) contains a polyadenylation signal, and for production of full-length rna the version of this at the 5' end of the template must be bypassed by rna polymerase, but the version at the 3' end must be recognized. this implies that the position of the polyadenylation signal determines ... | 2008 | 2366867 |
| large bottleneck size in cauliflower mosaic virus populations during host plant colonization. | the effective size of populations (ne) determines whether selection or genetic drift is the predominant force shaping their genetic structure and evolution. despite their high mutation rate and rapid evolution, this parameter is poorly documented experimentally in viruses, particularly plant viruses. all available studies, however, have demonstrated the existence of huge within-host demographic fluctuations, drastically reducing ne upon systemic invasion of different organs and tissues. notably, ... | 2008 | 18846207 |
| a maltose transporter from apple is expressed in source and sink tissues and complements the arabidopsis maltose export-defective mutant. | prior to the cytosolic synthesis of transport sugars during transitory starch utilization, intermediate products of starch breakdown, such as maltose, must be exported from chloroplasts. recent work in arabidopsis indicates that a novel transporter mediates maltose transfer across the chloroplast inner envelope membrane. we cloned a gene from an apple cdna library that is highly homologous with the arabidopsis maltose transporter, mex1. expression levels of mdmex determined by real-time pcr were ... | 2008 | 18776201 |
| antisense suppression of an acid invertase gene (mai1) in muskmelon alters plant growth and fruit development. | to unravel the roles of soluble acid invertase in muskmelon (cucumis melo l.), its activity in transgenic muskmelon plants was reduced by an antisense approach. for this purpose, a 1038 bp cdna fragment of muskmelon soluble acid invertase was expressed in antisense orientation behind the 35s promoter of the cauliflower mosaic virus. the phenotype of the antisense plants clearly differed from that of control plants. the transgenic plant leaves were markedly smaller, and the stems were obviously t ... | 2008 | 18641398 |
| nuclear import of camv p6 is required for infection and suppression of the rna silencing factor drb4. | replication of cauliflower mosaic virus (camv), a plant double-stranded dna virus, requires the viral translational transactivator protein p6. although p6 is known to form cytoplasmic inclusion bodies (viroplasms) so far considered essential for virus biology, a fraction of the protein is also present in the nucleus. here, we report that monomeric p6 is imported into the nucleus through two importin-alpha-dependent nuclear localization signals, and show that this process is mandatory for camv in ... | 2008 | 18615098 |
| overexpression of an h+-ppase gene from thellungiella halophila in cotton enhances salt tolerance and improves growth and photosynthetic performance. | salinity is one of the major environmental factors limiting plant growth and productivity. an h(+)-ppase gene, tsvp from thellungiella halophila, was transferred into cotton (gossypium hirsutum) in sense and antisense orientations under control of the cauliflower mosaic virus (camv) 35s promoter. southern and northern blotting analysis showed that the sense or antisense tsvp were integrated into the cotton genome and expressed. transgenic plants overexpressing the vacuolar h(+)-ppase were much m ... | 2008 | 18550626 |
| accumulation of isoflavone genistin in transgenic tomato plants overexpressing a soybean isoflavone synthase gene. | isoflavones are legume-specific flavonoids best known for their potential cancer preventive and phytoestrogenic properties. in this study, we attempted to engineer the isoflavone pathway in the popular fruit crop tomato (solanum lycopersicum l). tomato plants were transformed with a soybean (glycine max l) isoflavone synthase (gmifs2) cdna under the control of the cauliflower mosaic virus 35s promoter. lc-ms/ms analysis demonstrated the presence of genistin (genistein 7-o-glucoside) as the major ... | 2008 | 18540614 |
| gaussia-luciferase as a sensitive reporter gene for monitoring promoter activity in the nucleus of the green alga chlamydomonas reinhardtii. | for the model organism chlamydomonas reinhardtii, a codon-adapted gene variant of the extracellular luciferase of gaussia princeps was generated as a sensitive molecular tool to study gene expression from the nuclear genome. in the past, monitoring promoter activity in chlamydomonas employing the commonly used luciferase encoded by renilla reniformis was hampered due to the detection limit of the reporter assay, especially if analyzing weak promoters. in this work, the expression of gaussia-luci ... | 2008 | 18516621 |
| the hd-zip iv gene gahox1 from cotton is a functional homologue of the arabidopsis glabra2. | most of the plant homeodomain-containing proteins play important roles in organ patterning and development, and arabidopsis glabra2 (gl2), a member of the class iv homeodomain-leucine zipper (hd-zip) proteins, is a trichome and non-root hair cell regulator. here we report the analysis of two cotton homeodomain-containing proteins, gahox1 and gahox2, isolated from the diploid cotton gossypium arboreum. both gahox1 and gahox2 belong to the class iv hd-zip family. when expressed under the control o ... | 2008 | 18507789 |
| glycine-rich rna-binding protein 7 affects abiotic stress responses by regulating stomata opening and closing in arabidopsis thaliana. | despite the fact that glycine-rich rna-binding proteins (grps) have been implicated in the responses of plants to environmental stresses, their physiological functions and mechanisms of action in stress responses remain largely unknown. here, we assessed the functional roles of grp7, one of the eight grp family members in arabidopsis thaliana, on seed germination, seedling growth, and stress tolerance under high salinity, drought, or cold stress conditions. the transgenic arabidopsis plants over ... | 2008 | 18410480 |
| electrochemical biosensor for the detection of cauliflower mosaic virus 35 s gene sequences using lead sulfide nanoparticles as oligonucleotide labels. | lead sulfide (pbs) nanoparticles were synthesized in aqueous solution and used as oligonucleotide labels for electrochemical detection of the 35 s promoter from cauliflower mosaic virus (camv) sequence. the pbs nanoparticles were modified with mercaptoacetic acid and could easily be linked with camv 35 s oligonucleotide probe. target dna sequences were covalently linked on a mercaptoacetic acid self-assembled gold electrode, and dna hybridization of target dna with probe dna was completed on the ... | 2008 | 18381196 |
| a new technique for activation tagging in arabidopsis. | we have created and applied to arabidopsis thaliana a new system of two vectors. the first vector (penlox) is intended for activation tagging and contains a multimerized transcriptional enhancer from the cauliflower mosaic virus (camv) 35s gene in t-dna flanked by two loxp-sites and the second vector (pcre) contains the cre gene. using penlox we have generated more than a hundred mutants resistant to the herbicide ammonium glufosinate, and about ten helper-lines resistant to the antibiotic hygro ... | 2008 | 18353569 |
| detection of nonauthorized genetically modified organisms using differential quantitative polymerase chain reaction: application to 35s in maize. | detection of nonauthorized genetically modified organisms (gmos) has always presented an analytical challenge because the complete sequence data needed to detect them are generally unavailable although sequence similarity to known gmos can be expected. a new approach, differential quantitative polymerase chain reaction (pcr), for detection of nonauthorized gmos is presented here. this method is based on the presence of several common elements (e.g., promoter, genes of interest) in different gmos ... | 2008 | 18346452 |
| polyubiquitin promoter-based binary vectors for overexpression and gene silencing in lotus japonicus. | in this study, we compared the transcriptional activities between cauliflower mosaic virus (camv)35s promoter and polyubiquitin (ljubq1) promoter from lotus japonicus using beta-glucuronidase (gus) reporter gene in transgenic plants of l. japonicus. the promoter analysis demonstrated that the ljubq1 promoter possessed higher activity than the camv35s promoter in leaves, stems, roots, nodules, and pollen. finally, we created gateway conversion technology-compatible binary vectors for over-express ... | 2008 | 18321183 |
| expression of exogenous genes under the control of endogenous hsp70 and cab promoters in the closterium peracerosum-strigosum-littorale complex. | a unicellular charophyte alga, closterium peracerosum-strigosum-littorale complex (c. psl. complex), has been studied in order to obtain basic information regarding sexual reproduction in plants. systems for gene introduction and transient expression were developed for endogenous genes using phleomycin resistance (ble) and chlamydomonas green fluorescent protein (cgfp) genes as selection markers. these genes have codon usage similar to that of genes in the c. psl. complex. to drive these genes s ... | 2008 | 18308758 |
| overexpression of the non-canonical aux/iaa genes causes auxin-related aberrant phenotypes in arabidopsis. | degradation of aux/iaa proteins which are triggered by the ubiquitin ligase complex containing the auxin f-box receptors (afbs), is thought to be the primary reaction of auxin signaling. upon auxin perception, afbs bind domain ii of aux/iaa proteins that is conserved in most of the 29 family members in arabidopsis. however, iaa20 and iaa30 lack domain ii. furthermore, iaa31, which forms a single clade with iaa20 and iaa30 in aux/iaa protein family, has a partially conserved domain ii, which cont ... | 2008 | 18298415 |
| quantitative single-letter sequencing: a method for simultaneously monitoring numerous known allelic variants in single dna samples. | pathogens such as fungi, bacteria and especially viruses, are highly variable even within an individual host, intensifying the difficulty of distinguishing and accurately quantifying numerous allelic variants co-existing in a single nucleic acid sample. the majority of currently available techniques are based on real-time pcr or primer extension and often require multiplexing adjustments that impose a practical limitation of the number of alleles that can be monitored simultaneously at a single ... | 2008 | 18291029 |
| assessment of camv-mediated gene silencing and integration of camv into gm plants with a 35s rna promoter. | four gm plant species (arabidopsis thaliana, brassica napus, nicotiana benthamiana and n. tabacum), each expressing the gene encoding the jellyfish green fluorescent protein (gfp) regulated by the cauliflower mosaic virus (camv) 35s rna promoter, were assessed for the extent of transgene silencing and viral genome integration following infection by camv. the first three species are systemic hosts of camv, while n. tabacum is only a local host for a few strains of camv. a generalized systemic sil ... | 2008 | 18289501 |
| overexpression of zeaxanthin epoxidase gene enhances the sensitivity of tomato psii photoinhibition to high light and chilling stress. | a tomato (lycopersicon esculentum mill.) zeaxanthin epoxidase gene (leze) was isolated. the deduced amino acid sequence of leze showed high identities with zeaxanthin epoxidase in other plant species. northern blot analysis showed that the mrna accumulation of leze in the wild-type (wt) was not induced by light and temperature but regulated by the diurnal rhythm. the sense transgenic plants were obtained under the control of the cauliflower mosaic virus 35s promoter (35s-camv). northern and west ... | 2008 | 18275469 |
| constitutive expression of a meiotic recombination protein gene homolog, ostop6a1, from rice confers abiotic stress tolerance in transgenic arabidopsis plants. | plant productivity is greatly influenced by various environmental stresses, such as high salinity and drought. earlier, we reported the isolation of topoisomerase 6 homologs from rice and showed that over expression of ostop6a3 and ostop6b confers abiotic stress tolerance in transgenic arabidopsis plants. in this study, we have assessed the function of nuclear-localized topoisomerase 6 subunit a homolog, ostop6a1, in transgenic arabidopsis plants. the over expression of ostop6a1 in transgenic ar ... | 2008 | 18071708 |
| protoplast isolation and transient gene expression in switchgrass, panicum virgatum l. | transient assay systems using protoplasts have been utilized in several plant species and are a powerful tool for rapid functional gene analysis and biochemical manipulations. a protoplast system has not been used in switchgrass (panicum virgatum l.), even though it is a bioenergy crop that has received considerable attention. here we report the first protocol to isolate large numbers of viable protoplasts from both leaves and roots of two switchgrass genotypes. furthermore, we demonstrate trans ... | 2008 | 18064611 |
| tomato leaf curl virus satellite dna as a gene silencing vector activated by helper virus infection. | tomato leaf curl virus (tlcv) satellite dna (sat-dna) constructs containing functional segments of the cauliflower mosaic virus (camv) 35s promoter, replicate in tobacco in the presence of helper tlcv and silence gus activity in transgenic tobacco plants containing a camv 35s-gus expression cassette. we have analysed these plants for evidence of the hallmarks of silencing. the gus transcript was not detectable in the leaves of gus-silenced tobacco plants. these plants contained sirnas of approxi ... | 2008 | 18514962 |
| transformation by t-dna integration causes highly sterile phenotype independent of transgenes in arabidopsis thaliana. | agrobacterium tumefaciens-mediated gene transformation caused highly sterile phenotype in t1 transgenic populations of arabidopsis thaliana. the phenomenon occurred independent of the genes and construct types used for transformation. the occurring frequency is less than 10% and the phenotype is inheritable. intensive examination elucidated that the sterility is due to indehiscence or delayed dehiscence of the anthers during the flowering stage, resulting from the reduced or disordered endotheci ... | 2008 | 18521611 |
| agrobacterium tumefaciens-induced bacteraemia does not lead to reporter gene expression in mouse organs. | agrobacterium tumefaciens is the main plant biotechnology gene transfer tool with host range which can be extended to non-plant eukaryotic organisms under laboratory conditions. known medical cases of agrobacterium species isolation from bloodstream infections necessitate the assessment of biosafety-related risks of a. tumefaciens encounters with mammalian organisms. here, we studied the survival of a. tumefaciens in bloodstream of mice injected with bacterial cultures. bacterial titers of 10(8) ... | 2008 | 18523638 |
| expression of insect (microdera puntipennis dzungarica) antifreeze protein mpafp149 confers the cold tolerance to transgenic tobacco. | to elucidate the function of antifreeze protein from microdera puntipennis dzhungarica for freezing stress tolerance in plant, the construct of mpafp149 gene with the signal peptide sequence responsible for secreting the native mpafp149 into the apoplast space under control of a cauliflower mosaic virus 35s promoter was introduced into tobacco by agrobacterium tumefaciens-mediated transformation. the observation of immunogold localization by tem (transmission electron microscope) showed that the ... | 2008 | 18528694 |
| construction of an infectious full-length cdna clone of potato virus m. | an infectious full-length cdna clone of potato virus m (pvm) was produced. total rna was extracted from pvm-infected nicotiana hesperis plants and used for cdna synthesis. subsequent rt-pcr produced two dna fragments of about 5.5 and 3.2 kbp, which were ligated downstream of an enhanced 35s cauliflower mosaic virus promoter. after cloning of the enhanced 35s promoter with the pvm sequence into a modified pbin19 plasmid and electroporation of agrobacterium tumefaciens, the agroinoculated pvm full ... | 2008 | 18543062 |
| overexpression of the arabidopsis thaliana eds5 gene enhances resistance to viruses. | the arabidopsis thaliana enhanced disease susceptibility 5 gene (eds5) is required for salicylic acid (sa) synthesis in pathogen-challenged plants. sa and eds5 have an important role in the arabidopsis rcy1 gene-conferred resistance against the yellow strain of cucumber mosaic virus [cmv(y)], a bromoviridae, and hrt-conferred resistance against the tombusviridae, turnip crinkle virus (tcv). eds5 expression and sa accumulation are induced in response to cmv(y) inoculation in the rcy1-bearing ecot ... | 2008 | 18557905 |
| efficient expression of a toxoplasma gondii dense granule gra4 antigen in tobacco leaves. | a his-tagged truncated version of toxoplasma gondii dense granule 4 protein (gra4(163-345)) was transiently expressed in tobacco leaves. two genetic constructions were used to accomplish this goal. in one of them, based in a potato virus x (pvx) amplicon, the sequence encoding his-gra4(163-345) was placed under control of an additional pvx coat protein subgenomic promoter. in the other, the same sequence was fused to an apoplastic transport signal and placed under the direction of the cauliflowe ... | 2008 | 18588877 |
| evaluation of constitutive viral promoters in transgenic soybean roots and nodules. | the efficiency of beta-glucuronidase (gus) expression was evaluated with five viral promoters to identify the most suitable promoter or promoters for use in soybean hairy roots, including applications to study the symbiotic interaction with bradyrhizobium japonicum. levels of gus activity were fluorimetrically and histochemically assayed when the gus (uida) gene was driven by the cauliflower mosaic virus (camv) 35s promoter and enhanced 35s (e35s) promoter, the cassava vein mosaic virus (csvmv) ... | 2008 | 18616399 |
| a novel lipoxygenase gene from developing rice seeds confers dual position specificity and responds to wounding and insect attack. | oslox1 is a novel full-length cdna isolated from developing rice seeds. we have examined its biochemical properties and expression patterns. the protein has dual positional specificity, as it releases both c-9 and c-13 oxidized products in a 4:3 ratio. oslox1 transcripts were detected at low abundance in immature seeds and newly germinated seedlings, but accumulate rapidly and transiently in response to wounding or brown planthopper (bph) attack, reaching a peak 3 h after wounding and 6 h after ... | 2008 | 18185911 |
| translation of ciap2 mrna is mediated exclusively by a stress-modulated ribosome shunt. | during cellular stress, translation persists or increases for a number of stress-responsive proteins, including cellular inhibitor of apoptosis 2 (ciap2). the ciap2 transcript includes a very long (2.78-kb) 5' untranslated region (utr) with an unusually high number of upstream augs (uaugs), i.e., 64, and a stable predicted secondary structure (deltag congruent with -620 kcal/mol) that should completely block conventional scanning-dependent translation initiation. this region did not facilitate i ... | 2008 | 18195037 |
| plastid targeting strategies for cyanophycin synthetase to achieve high-level polymer accumulation in nicotiana tabacum. | the production of biodegradable polymers in transgenic plants is an important challenge in plant biotechnology; nevertheless, it is often accompanied by reduced plant fitness. in order to decrease the phenotypic abnormalities caused by cytosolic production of the biodegradable polymer cyanophycin, and to increase polymer accumulation, four translocation pathway signal sequences for import into chloroplasts were individually fused to the coding region of the cyanophycin synthetase gene (cpha(te)) ... | 2008 | 18282176 |
| isolation of a strong arabidopsis guard cell promoter and its potential as a research tool. | abstract: | 2008 | 18284694 |
| cross-species functionality of pararetroviral elements driving ribosome shunting. | cauliflower mosaic virus (camv) and rice tungro bacilliform virus (rtbv) belong to distinct genera of pararetroviruses infecting dicot and monocot plants, respectively. in both viruses, polycistronic translation of pregenomic (pg) rna is initiated by shunting ribosomes that bypass a large region of the pgrna leader with several short (s)orfs and a stable stem-loop structure. the shunt requires translation of a 5'-proximal sorf terminating near the stem. in camv, mutations knocking out this sorf ... | 2008 | 18286203 |
| excision of selectable marker gene from transgenic tobacco using the gm-gene-deletor system regulated by a heat-inducible promoter. | to excise a selectable marker gene from transgenic plants, a new binary expression vector based on the 'genetically modified (gm)-gene-deletor' system was constructed. in this vector, the gene coding for flp site-specific recombinase under the control of a heat shock-inducible promoter hsp18.2 from arabidopsis thaliana and isopentenyltransferase gene (ipt), as a selectable marker gene under the control of the cauliflower mosaic virus 35s (camv 35s) promoter, were flanked by two loxp/frt fusion s ... | 2008 | 18345518 |
| development of gene expression system in a marine diatom using viral promoters of a wide variety of origin. | promoter sequences of the cytomegalovirus (pcmv), the rous sarcoma virus long terminal repeat (prsv-ltr) and the cauliflower mosaic virus 35s (pcamv35s) were ligated with the beta-glucuronidase (gus) gene, uida, and were introduced into cells of the marine diatom, phaeodactylum tricornutum. transformants were selected on a 100 mg l(-1) zeocin plate, and zeocin-resistant clones were further selected by the occurrence of gus activity. two to 10 gus-positive clones were obtained, and gus activities ... | 2008 | 18346072 |
| [inhibition of agrobacterial oncogenes expression by means of antisense rna]. | plant's infection with soil bacteria agrobacterium tumefaciens lead to tumour formation, so called crown galls. the reason of tumorigenesis is integration of agrobacterial genes for phytohormone synthesis auxins and cytokinins in plant genome, the most important of them are iaam and ipt. obtaining of transgenic plants able to inhibit these genes expression, creates conditions for producing of plants resistant to crown gall disease. with this purpose single and double transformants of tobacco pla ... | 2008 | 18389635 |
| overexpression of a new rice vacuolar antiporter regulating protein osarp improves salt tolerance in tobacco. | we examined the function of the rice (oryza sativa l.) antiporter-regulating protein osarp by overexpressing it in tobacco (nicotiana tabacum l.). in public databases, this protein was annotated as a putative os02g0465900 protein of rice. the osarp gene was introduced into tobacco under the control of the cauliflower mosaic virus 35s promoter. the transformants were selected for their ability to grow on medium containing kanamycin. incorporation of the transgene in the genome of tobacco was conf ... | 2008 | 18420595 |