Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [metal concentrations in fishes of the rednitz river]. | we investigated 39 fish species (eel, brown trout, chub, carp bream, roach, perch, pike etc.) from the river rednitz (north bavaria) for content of the metals mercury, lead, cadmium, arsenic, selenium and zinc. the residue tolerance level for mercury (1 mg/kg) and the standard value of the federal public health office for lead (0.5 mg/kg) and cadmium (0.05 mg/kg) have not been exceeded in any case. because a small selenium concentration gives protection against the toxicity of mercury, we also h ... | 1985 | 4024745 |
| a comparative study of tropomyosin from vertebrate ventricles. | a comparative study of vertebrate ventricle tropomyosin has been carried out from the viewpoint of molecular evolution. the ventricles containing one-component tropomyosin were generally known, and in this paper those containing two components were also found in 8 species among mammals, reptiles, amphibia, and fish, but not among birds. the two components were concluded to be authentic tropomyosin and not artifacts since they showed lower electrophoretic mobilities in the presence of urea, and t ... | 1985 | 4030730 |
| [phylo- and ontogenic characteristics of lipid peroxidation in the retina of vertebrates]. | phylo- and ontogenetic aspects of lipid peroxidation and antioxidative enzyme system in the retina of vertebrates were studied. it was established that both the intensity of lipid peroxidation and the activity of glutathione peroxidase in the retina of different vertebrate animals (carp, frog, tortoise, pigeon, rabbit) considerably diminished with evolution. the differences in the intensity of lipid peroxidation and the activity of glutathione peroxidase between dark- and light-adapted retinas a ... | 1985 | 4052609 |
| ca2+-specific fluorescence changes in n-dansylaminoethyl-labelled oncomodulin. | rat oncomodulin (isoelectric point (pi) = 3.9) and homologous carp parvalbumin (pi = 3.95) was labelled at cys-18 which is located in the ab loop, with the fluorescent reagent n-dansylaziridine. the fluorescence spectrum of the labelled parvalbumin was insensitive to both mg2+ and ca2+ binding. in contrast the dansyl fluorescence emission spectrum of labelled oncomodulin was quenched approximately 20% by ca2+ in the presence of physiological concentrations of mg2+. approximately 90% of the ca2+- ... | 1985 | 4075234 |
| antigen localization in the lymphoid organs of carp (cyprinus carpio). | a brief morphological description is given of the spleen, head kidney and trunk kidney, the three peripheral lymphoid organs of carp. an antigen-localization study was carried out using aeromonas hydrophila (a cellular bacterial antigen) and an indirect immunofluorescence test. examination of the lymphoid organs at various times after injection (up to 12 months) showed that antigen was at first present in splenic ellipsoids and in solitary phagocytic cells in the spleen, head and trunk kidney. l ... | 1985 | 4075373 |
| spectroscopic studies of protein-heme interactions accompanying the allosteric transition in methemoglobins. | resonance raman, optical absorption, and circular dichroism spectroscopic techniques have been used to examine the effect of the addition of inositol hexaphosphate (ihp) to a series of carp and human methemoglobin derivatives. markers of spin equilibrium in the high-frequency region (1450-1650 cm-1) of the resonance raman spectrum yield high/low-spin ratios consistent with direct magnetic susceptibility measurements. changes in the low-frequency region (100-600 cm-1) of the resonance raman spect ... | 1985 | 4084499 |
| purification and partial characterization of the glutathione s-transferases in carp liver, and their interaction with 2,4-dichlorophenoxyacetic acid and 1,4-benzoquinone. | the hepatic glutathione s-transferase (gst) activity in the cytosol of the freshwater fish carp (cyprinus carpio) was enriched by glutathione affinity chromatography. the anionic (gst a1-a3) and cationic (gst c1-c3) isoenzymes were then separated in two chromatofocusing steps. sds electrophoresis showed gst c1 to be a heterodimer with subunits of mr 25,000 and 28,000, and all other isoenzymes to be homodimers with subunits of mr 25,400. they were partially characterized by different biochemical ... | 1985 | 4091850 |
| electrophoretic and immunological study of myosin light chains from freshwater teleost fishes. | white muscle myosin light chains from nine freshwater teleosts show a qualitative and quantitative variability on page without phylogenetic correlation. they look different from their higher vertebrate counterparts mainly with regard to electric charge and relative amounts of alkali light chains corresponding to various contents of isoenzymic forms of white muscle myosin. antibodies against carp white myosin lc1 recognize almost entirely white muscle lc1 from the other fishes and to a lesser deg ... | 1985 | 4092438 |
| [specificity of carp antihuman myeloma protein sera depending on the holding temperature]. | while in carps kept in warm water (28 degrees c) and immunized with a human myeloma protein a challenge leads to an increase, the antibody titres in carps kept in cold water (14 degrees c) are changed only unsignificantly. antibody titres of carps immunized once respectively three times and kept in cold water are not markedly different. in testing the specificity of the antisera taken from each of the three groups no differences could be recognized. with carp anti-iggzei sera using the passive h ... | 1985 | 2936221 |
| so-called interplexiform cells immunoreactive to tyrosine hydroxylase or somatostatin in rat retina. | the morphology of so-called interplexiform (ip) cells immunoreactive to tyrosine hydroxylase (th) or somatostatin (som) in the rat retina was described in comparison with those in the carp retina. in frozen cross-sections of the rat retina, many processes of th-like immunoreactive cells were found to extend toward the outer plexiform layer (opl), forming a thin layer of network fibers. a few of them further extended into the photoreceptor cell layer; such fibers were never found in the carp reti ... | 1985 | 2864980 |
| effects of acidic amino acid antagonists upon the spectral properties of carp horizontal cells: circuitry of the outer retina. | the acidic amino acid receptor antagonists, alpha-methylglutamate and alpha-aminoadipate, were applied to the carp retina to study their effects upon the spectral properties of horizontal cells and to elucidate the synaptic connections between horizontal cells and cones. application of these antagonists strongly hyperpolarized the l-type cone horizontal cells and reduced the responses of these horizontal cells to red light more than to blue light. application of co2+ ions to the retina, a proced ... | 1985 | 2865339 |
| histaminergic mechanisms in the vertebrate visual system. | this paper presents and summarizes our recent results on histamine (hi) in vertebrate visual system. the data include: 1. hi distribution, localization, synthesis, uptake and metabolism in retina, optic nerve, choroid and brain of carp, hen, rabbit, cow, 2. distribution of hi in human eyes, 3. 3h-mepyramine binding in the bovine retina, and hi dependent camp generating system in the rabbit eye, 4. effect of light stimulation on hi content in the rabbit retina, optic nerve and brain, 5. diurnal v ... | 1985 | 2870485 |
| production of monoclonal antibodies directed against antigenic determinants common to the alpha- and beta-chain of bovine brain s-100 protein. | balb/c mice were immunized with s-100 protein, which was isolated from bovine brain. the first fusion resulted in the cloning of three stable hybridoma lines (s1-61-64, s1-61-65, s1-87-4) that produced monoclonal antibodies against s-100 protein. the hybridoma lines obtained from a second fusion (s2-20, s2-95) were not stable and antibody production ceased early during cloning. immunoblotting results showed that all antibodies reacted with antigenic determinants shared by both the alpha- and bet ... | 1985 | 2578587 |
| phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding. | of eight monoclonal antibodies raised against human transferrin, one (h.tf-14) cross reacted with pig and rabbit transferrins and one (h.tf-1) showed cross-reactivity with horse and dog transferrins. while rabbit and pig transferrins exhibited the same patterns of binding to molt-3 cell receptors as human and horse transferrins, binding of mouse and dog transferrins was weaker and bovine and carp transferrins gave entirely negative results. the results of these competitive binding experiments we ... | 1985 | 2578807 |
| adenohypophysial cell types in an indian freshwater major carp, catla catla (hamilton). | the different adenocytes in an indian freshwater major carp, catla catla (hamilton) have been studied by employing various modern staining techniques. altogether, 8 different cell types have been reported in the adenohypophysis of the aforesaid fish. variations in the staining intensities of the cell types with the different techniques adopted have been detailed. | 1985 | 2581848 |
| localization of immunoreactive synthetic atrial natriuretic factor (anf) in the heart of various animal species. | the localization of two synthetic fragments of the c-terminal portion of atrial natriuretic factor: arg 101-tyr 126 which displays full biological activity and leu 94-arg 109 which is completely devoid of biological activity, has been investigated by immunohisto- and immunocytochemical methods in the heart of mammals (rat, mouse, guinea pig, hamster, rabbit, cat, dog, man) and nonmammalian vertebrates toad (bufo marinus), frog (rana catesbeiana), fish (cyprinus carpio, puntius schwanenfeldi, cic ... | 1985 | 3158698 |
| reexamination of the evidence for paramagnetism in oxy- and carbonmonoxyhemoglobins. | experiments have been carried out in an attempt to confirm previous reports of paramagnetism in the oxy- and carbonmonoxy derivatives of human and carp hemoglobin. when care is taken to ensure complete saturation of the hemoglobins with ligand and the diamagnetic contributions of all of the buffers are carefully evaluated, these hemoglobin derivatives are found to have the same gram susceptibilities as exhibited by a set of metal free proteins, suggesting that they are fully diamagnetic. | 1985 | 2982138 |
| human chorionic gonadotrophin and immature fish ovary: characterization and mechanism of the in vitro stimulation of cyclic adenosine monophosphate accumulation. | human chorionic gonadotrophin (hcg) increased cyclic adenosine monophosphate (camp) in pieces of eel ovary in vitro. although the ed50 (about 0.1 micrograms/ml) was the same as for carp gonadotrophin (cgth), the maximal stimulation (close to 3 times basal level) was about 10 times lower than that for the fish hormone. the mechanism of this atypical action of hcg was studied using different approaches. human cg did not inhibit the action of cgth. desensitization experiments (cgth or hcg being inj ... | 1985 | 2985465 |
| purification and properties of carp (cyprinus carpio) muscle calpain ii (high-ca2+-requiring form of calpain). | calpain (ca2+-dependent cysteine proteinase) was purified to apparent homogeneity from carp muscle by the method of deae-cellulose, hydroxylapatite and ultrogel aca 34 column chromatographies. the purified enzyme is classified as calpain ii (high-ca2+-requiring form of calpain) from the effects of ca2+ concentration, ph and the antibiotics on the activity. carp muscle calpain ii was inhibited by rat liver calpastatin, the specific inhibitor for calpain. it is probable that the calpain-calpastati ... | 1985 | 2992871 |
| mutual inhibitory effect of calpastatins on calpains from carp muscle, carp erythrocytes and rat liver. | the inhibitory effect of calpastatin (specific inhibitor for calpain) on calpain (ca2+-dependent cysteine proteinase, ec 3.4.22.17) was examined using carp muscle, carp erythrocytes and rat liver preparations. a mutual inhibitory effect between calpains and calpastatins from different tissues and species was observed. the conservation of the inhibitory effect of calpastatin on calpain among vertebrates suggests that the calpain-calpastatin system may play a biologically fundamental and common ro ... | 1985 | 2992872 |
| viral diseases of fish: first report of carp pox in golden ide (leuciscus idus) in north america. | carp pox, a putative viral disease exotic to north america, occurred in golden ide 1 yr after the fish were imported into the united states from the federal republic of germany. the raised, white, plaque-like lesions, which occurred on about 5% of the fish, healed spontaneously and caused no mortality. electron micrographs showed herpesvirus-like particles associated with lesion specimens; however, no infectious viruses were detected in tests with seven warmwater fish cell lines. | 1985 | 2993681 |
| uptake and distribution of radioiodine, and the effect of ambient nitrate, in some fish species. | 125i uptake by the thyroid was most pronounced in the smelt (osmerus eperlanus), perch (perca fluviatilis), rainbow trout (salmo gairdneri), roach (rutilus rutilus) and bleak (alburnus alburnus). unlike other tissues, only the muscle in the species studied no relative accumulation of 125i as compared to the ambient water. the crusian carp (cyprinus carassius) indicated the lowest levels of accumulation of 125i among the species tested. thyroid radioiodine concentration was independent of the len ... | 1985 | 2858311 |
| surface markers of male germ cells in early development of carp (cyprinus carpio l.) and the blood-testis barrier in fish. a study with monoclonal antibodies and horseradish peroxidase (hrp). | 1985 | 4028174 | |
| peripheral and central distribution of major branches of the facial taste nerve in the carp. | the major pathways of the peripheral facial taste system in the carp, cyprinus carpio, are the maxillary (max), mandibular (mand), palatine (pal) and recurrent nerve rami. the peripheral distribution of the sensory fibers of these branches (b) was determined by means of electrophysiological techniques. max.b., mand.b. and pal.b., each of which arises from the gasserian-geniculate ganglionic complexes, were found to innervate respectively, the upper lip and the adjacent skin, the internal and ext ... | 1985 | 3978434 |
| uptake and transport of intact macromolecules in the intestinal epithelium of carp (cyprinus carpio l.) and the possible immunological implications. | two protein antigens, horseradish peroxidase (hrp) and ferritin, have been administered to the digestive tract of carp. electron-microscopical observations reveal considerable absorption of both antigens in the second segment of the gut (from 70 to 95% of the total length) and also, although to a lesser extent, in the first segment (from 0 to 70% of the total length). even when administered physiologically with food, a large amount of ferritin is absorbed by enterocytes in the second gut segment ... | 1985 | 3986879 |
| separation and partial characterization by high-performance liquid chromatography and radioimmunoassay of different forms of melanocyte-stimulating hormone from fish (cyprinidae) neurointermediate lobes. | from neurointermediate lobe (nil) extracts of two species of cyprinidae, carassius auratus and cyprinus carpio, several peptides were separated by high-performance liquid chromatography (hplc) on a c18 mubondapak column eluted with a methanol/acetic acid/triethylamine mixture. monitoring all fractions by radioimmunoassay (ria) with an antibody against melanocyte-stimulating hormone (msh) c terminal gave positive reactions for fractions 7, 11-12, 15-16, 23-24, and 25-27. for further characterizat ... | 1985 | 2984088 |
| erythrocytic magnesium in freshwater fishes. | erythrocytic magnesium in rainbow and speckled trout (salmo gairdneri, salvelinus fontinalis) carp (cyprinus carpio) and goldfish (carassius auratus) was examined by correlation analyses involving weight, hemoglobin content, plasma magnesium, erythrocytic sodium, potassium, calcium, chloride and unidentified anion concentration and chloride equilibrium potential, and in relation to possible diurnal and seasonal variation as well as with respect to constant, diurnally cycling and progressively in ... | 1985 | 4046640 |
| the structure of the kidney from the freshwater teleost carassius auratus. | the structure of the kidney of the crucian carp (carassius auratus; a freshwater teleost, cypriniformes) was studied by means of reconstruction from serial paraffin and semithin sections. in c. auratus, the wolffian duct traverses the entire kidney. at various levels collecting ducts of different length and thickness join the wolffian duct at right angles. each collecting duct accepts a large number of connecting tubules, which are established by the joining of many nephrons. a regular pattern c ... | 1985 | 3985356 |
| crystal structure study of opsanus tau parvalbumin by multiwavelength anomalous diffraction. | the crystal structure of a small calcium-binding protein, the parvalbumin iiif from opsanus tau in which tb was substituted for ca, has been analysed by multiwavelength anomalous diffraction. data at a resolution of 2.3 a were collected at three wavelengths near the l3 absorption edge of tb (1.645-1.650 a), using the synchrotron radiation emitted by a storage ring and a multiwire proportional counter. the phases of the reflections were determined from this single derivative, without native data. ... | 1985 | 3965297 |
| the stratum album centrale in the optic tectum of cyprinus carpio. | the most outstanding characteristics of the stratum album centrale of the optic tectum of cyprinus carpio were analyzed by light and electron microscopy, with special reference to the morphology and ultrastructure of the neurons with cell bodies in this stratum. neurons belonging to the stratum album centrale itself are described, both vertical and horizontal in development, together with others which are found in this and the periventricular stratum. the possible involvement of this zone in the ... | 1985 | 3939080 |
| ultrastructural immunocytochemistry of gonadotrophs in the goldfish pituitary gland. | the immunocytochemical distribution of gonadotropin (gth) in the goldfish pituitary gland was studied applying the peroxidase-antiperoxidase (pap) method and the protein a-gold technique at light- and electron-microscopic levels, respectively, with an antiserum raised against silver carp gth. in the light-microscopic immunocytochemistry, pas-positive cells in the proximal pars distalis showed strong reaction with the antiserum. gold particles wee concentrated both on globules (large) and on gran ... | 1985 | 3884161 |
| effect of carp gonadotropin (cgth) and a fraction unabsorbed on concanavalin a-sepharose obtained from cgth on vitellogenesis in the hypophysectomized marine teleost gobius niger. | to investigate whether a differential potency on vitellogenesis exists between the carp gonadotropin (cgth) and fraction i-cgth (proteins from cgth unbound on concanavalin a-sepharose, which represent 5% of cgth in weight), hypophysectomized gobius niger were treated with the two hormonal preparations at the same level. vitellogenesis was checked for synthesis of vitellogenin and yolk incorporation in the ovary by means of immunological studies and histological techniques (light and electron mic ... | 1985 | 3882520 |
| daily changes in the gonadotropin levels and response of carp oocytes to hypophysial homogenate. | daily changes in carp gonadotropin levels in adult female carp and daily changes in carp oocyte sensitivity to carp hypophysial homogenate, in vitro and in vivo, were investigated. a total of three series of experiments were carried out. gonadotropin levels were radioimmunologically determined. the results of series 1 and 2 experiments were subjected to statistical analysis with the use of cosinors circle and ellipse of errors. it has been found that in the mature female carp in the pre-spawning ... | 1985 | 3870844 |
| correlations between photoresponse and morphology of amacrine cells in the carp retina. | 1985 | 3866153 | |
| electrical properties of horizontal cell network in the carp retina. | 1985 | 3866150 | |
| purification and some properties of a trypsin inhibitor from carp (cyprinus carpio) muscle. | a trypsin inhibitor was purified from carp muscle to apparent homogeneity by the successive chromatographies of deae-cellulose, deae-sepharose cl-6b, con a-sepharose, ultrogel aca 44 and hydroxylapatite. the mol. wt of the inhibitor was estimated to be 58,000 by sds-polyacrylamide gel electrophoresis or 50,000 by gel filtration. the inhibitor seemed to form a 1:1 stoichiometric complex with trypsin, alpha-chymotrypsin and elastase, respectively. carp muscle trypsin inhibitor was likely to be ide ... | 1985 | 3846514 |
| a comparison of quantitative ultrastructural and contractile characteristics of muscle fibre types of the perch, perca fluviatilis l. | quantitative ultrastructural and physiological parameters were investigated in three types of muscle fibres of perca fluviatilis: white fibres from the m. levator operculi anterior, pink (intermediate) fibres of the m. hyohyoideus and deep red fibres of the m. levator operculi anterior. times to peak tension and half relaxation times of isometric twitches increased in the mentioned order. the extent of contact between the t system and the sarcoplasmic reticulum and the relative volume and surfac ... | 1985 | 3837036 |
| [monoclonal antibodies against differentiation antigens of carp thymocytes (cyprinus carpio l.)]. | using the cell fusion technique we have produced monoclonal antibodies directed against carp thymocytes. the monoclonals revealed a different ability for binding to carp lymphocytes of various origin. the antibodies reacting only with lymphocytes seem to react with a membrane structure of a molecular weight of 200 000 to 250 000 daltons. | 1985 | 3158176 |
| detection and some properties of calpain ii (high-ca2+-requiring form of calpain) in carp (cyprinus carpio) erythrocytes. | in order to examine the existence of calpain i, a low (micromolar)-ca2+-requiring form of calpain, in fish tissues, carp erythrocytes were chosen as the experimental material, since only calpain i is known to exist in mammalian erythrocytes. by deae-cellulose chromatography, calpain and calpastatin (specific inhibitor for calpain) were separated from carp erythrocyte hemolysate. carp erythrocyte calpain is classified as calpain ii, a high (millimolar)-ca2+-requiring form of calpain, from the res ... | 1985 | 2992873 |
| latency of horizontal cell response in the carp retina. | the response latency of horizontal cells in the carp retina was investigated by means of the stimulus-response crosscorrelation using a randomly modulated light of various wavelengths. r/g type cells produced two response components with some difference in latency; short-latency hyperpolarising response to green light and long-latency depolarising one to red light. the latency difference could not be made smaller than 14 ms by increasing the intensity of test light. these results were reconciled ... | 1985 | 2992160 |
| [synaptic mechanisms of the responses of olfactory bulb neurons of the carp to odorants]. | analysis of synaptic responses to some odorants in 16 secondary olfactory neurons and 5 granule cells of carp olfactory bulb revealed that the periodic discharges of secondary neurons, synchronous with the induced waves, were generated on the basis of dendrodendritic inhibitory interaction with granule interneurons. the mechanisms of tonic, asynchronous discharges in some secondary neurons remained not quite clear. | 1985 | 2983240 |
| isoproterenol-induced desensitization to the positive inotropic effect of isoproterenol in ventricular strips isolated from carp heart (cyprinus carpio). | whether the positive inotropic effect of isoproterenol in ventricular strips of carp heart is altered by previous exposure to the agonist was studied. isoproterenol produced a concentration-dependent positive inotropic effect in these preparations which was competitively antagonized by propranolol. isoproterenol dose-response curves were shifted significantly downward and to the right after previous treatment with and removal of isoproterenol. the desensitization could be demonstrated after 5 mi ... | 1985 | 2866913 |
| the effects of pentachlorophenol, phenol and other pollutants on the liver of carp (cyprinus carpio l.). | liver tissue of carp was kept in roller tubes and the basal and epinephrine-induced release of glucose after 6 or 24 hr incubation were measured. the amount of liver glycogen after incubation was also determined. the liver was taken from carp treated in vivo with pollutants, mainly pcp or phenol, or was exposed to these pollutants in vitro. treatment of carp in vivo with 10-10(4) micrograms/l phenol reduced the basal and the epinephrine-induced release of glucose from the liver. treatment with l ... | 1985 | 2866901 |
| some observations on the influence of stress on ecg patterns in oreochromis mossambicus and cyprinus carpio. | electrocardiograms of undisturbed, resting fish as well as disturbed (stressed) fish were recorded by biotelemetry. both fish species showed marked changes in heartbeat patterns in response to stress with tilapia exhibiting the most pronounced changes. a theory is advanced to account for the marked changes in electrocardiogram patterns of disturbed fishes. | 1985 | 2866875 |
| effect of pesticides on the ldh activity and isoenzyme pattern of carp (cyprinus carpio l.) sera. | copper sulphate (cuso4), paraquat (pq) and methidathion (md) all caused tissue necrosis as demonstrated by increased levels of lactate dehydrogenase (ldh, ec 1.1.1.27) in blood sera. the main target of damage is shown by the isoenzymes appearing in the blood sera. the cuso4 treatment damaged mainly the heart and skeletal muscles. this is indicated by the increased ldh level and the m and h subunits appearing in the isoenzyme pattern of the serum. pq damaged first of all the gills and methidathio ... | 1985 | 2865066 |
| a thrombelastographic study of the effect of stress on the blood coagulation in cyprinus carpio (cyprinidae) and oreochromis mossambicus (cichlidae). | the normal blood coagulation in cyprinus carpio and oreochromis mossambicus was investigated by thrombelastography and standard thrombelastographs were obtained for each species. the effect of stress on blood coagulation was thereafter determined. the thrombelastograms indicated hypercoagulability under stress conditions. a prominent observation is the differences in the elasticity of the clot under various conditions. fibrinolysis occurred in all cases, except in the case of cyprinus carpio aft ... | 1985 | 2864206 |
| the effect of paraquat on the peroxide metabolism enzymes in erythrocytes of freshwater fish species. | the toxic effects of 10 ppm paraquat in vivo on the enzymes superoxide dismutase (sod), catalase (c), peroxidase (p), glutathione peroxidase (gsh-px) and on lipid peroxidation (lp) were estimated in erythrocytes of the carp, the tench and the crucian carp. paraquat caused activity enhancement of the peroxide metabolism enzymes and increase of the lipid peroxidation in the carp and the crucian carp. the enzyme activities and lipid peroxidation were dependent on the species and on the length of th ... | 1985 | 2861964 |
| accumulation of pollutants in fish. | the amount of radioactivity which derived from 14c-labeled pollutants was determined in liver, kidney, intestine, blood, muscle and gills of carp, exposed for 6, 24 and 72 hr to high external concentrations of urea, methanol, atrazine and pcp. the results allowed one to calculate roughly the uptake rate for these compounds. it was low for urea (0.055 micrograms/g per hr), higher for methanol (0.12) and atrazine (0.16) and highest for pcp (1.5). the bioaccumulation factors (bfs) were determined f ... | 1985 | 2861946 |
| induction of metal-binding proteins by zinc is accompanied by reduction of other proteins including antibodies. | exposure of carp lymphoid cells to zinc causes formation of metal-binding proteins (metallothioneins). metallothionein induction is accompanied by a suppression of the production of other proteins. antibodies are among the proteins which are decreased by zinc exposure. similar results from in vivo experiments indicate that zinc might affect even fish living in water still regarded as unpolluted. | 1985 | 2861052 |
| membrane lipid environment of carp brain and liver mitochondrial monoamine oxidase. | delipidation of carp liver mitochondria by treatment with methyl ethyl ketone (mek) or triton x-100 and then perchlorate greatly reduced mao activities. treatment with only triton x-100 resulted in less reduction in activity. the km values of the remaining activities were similar regardless of these treatments. the sensitivities towards clorgyline and l-deprenyl of the remaining activity in the triton x-100-treated residue and the phospholipase c-treated carp brain mitochondria were found to be ... | 1985 | 2861003 |
| sodium-dependent absorption of inorganic phosphate by the carp intestine. | concentrative absorption of inorganic phosphate observed in the stripped and everted intestine of the carp disappeared, when na concentration of the ringer solution was reduced from 150 to 7 mm, or when orthophosphate was replaced with metaphosphate. incubation in the ringer solution gassed with n2 instead of o2 also resulted in loss of the pi absorption. dinitrophenol significantly decreased the unidirectional pi flux from mucosa to serosa (jms) and increased the flux from serosa to mucosa (jsm ... | 1985 | 2858323 |
| in vitro absorption of inorganic phosphate and other electrolytes in the carp intestine. | net movements of water, na, cl, k, ca, mg and pi were measured in the stripped and everted intestine of the carp. a high concentrative absorbtion of pi was observed, whereas na and cl were absorbed almost isotonically. the rate of pi absorption was greater in the middle part than in the anterior or posterior part of the intestine. | 1985 | 2858287 |
| chemical analysis of internal environmental response of carp puntius stigma to ddt. | exposure of puntius stigma to sublethal dose of ddt (0.05 ppm) for 15 days decreased the liver concentrations of protein and dna from 65 +/- 0.91 to 63 +/- 0.78 mg/100 mg and 669 +/- 17 to 575 +/- 14 micrograms/100 mg, respectively whereas rna increased from 8214 +/- 318 to 8929 +/- 209 micrograms/100 mg. biotransformation products of this hydrocarbon interfered in the pathways of protein biosynthesis and induced proteolysis in hepatic tissues. the resulting changes were explained in the light o ... | 1985 | 2416702 |
| bioassay of fish gonadotrophin by ovarian mitochondrial cholesterol depletion. | murrel (channa punctatus bloch) ovarian tissues were incubated in vitro with or without piscine gonadotrophins and then subjected to subcellular fractionation followed by nonesterified cholesterol (cholesterol) assay. gonadotrophin from salmon (sg-g100), tilapia (tl1ms), and sturgeon (s27ms) depleted cholesterol in the mitochondrial fraction, whereas cholesterol remained unchanged in other subcellular fractions. aminoglutethimide, an inhibitor of mitochondrial cholesterol side-chain cleavage, bl ... | 1985 | 3988028 |
| dopamine inhibits calcium-independent gamma-[3h]aminobutyric acid release induced by kainate and high k+ in the fish retina. | kainic acid (ka) at micromolar concentrations stimulated the release of gamma-[3h]aminobutyric acid [( 3h]gaba) from a particulate fraction of the carp (cyprinus carpio) retina. the ka action was dose-dependent but ca2+-independent. a similar response was elicited by another glutamate receptor agonist, quisqualic acid, and high k+, but not by an aspartate agonist, n-methyl-d-aspartic acid. the stimulatory action of ka on the [3h]gaba release was selectively blocked by the ka blockers gamma-d-glu ... | 1985 | 3882885 |
| retinal growth in carp of the same age: density and number of dopamine neurons. | research has shown that an increase in the size of retina with fish growth is accompanied by a reduction in the density of monoamine neurons along with an increase in their cell number per retina. the age of fish, however, has not been considered in relation to the above. in the present study, juvenile carp (6.4 +/- 0.7 cm in body length), hatched 2 months earlier, were reared for 10 months under 2 different sets of conditions; one group was kept in a small aquarium at room temperature (rt, 9-26 ... | 1985 | 3986607 |
| structural studies of calcium-binding proteins using nuclear magnetic resonance. | lanthanide-shifted 1h nuclear magnetic resonance (nmr) spectroscopy has been used to compare the structure in solution of the ef-hand calcium-binding domains of four parvalbumins (isoelectric ph[pi] 3.95, 4.25, and 4.37 from carp, and pi from buffalo fish). these four parvalbumins are shown by nmr to have very similar structures at the level of resolution typical of x-ray structures. at the higher resolution possible by the lanthanide nmr technique, specific differences are noted between the pi ... | 1985 | 3978195 |
| [mitraspora cyprini fujita, 1912 (protozoa myxosporea bütschli, 1881) in the kidneys of carp from lower saxony pond farms]. | 1985 | 3977820 | |
| a bifurcate axon of horizontal cells in the carp retina. | in flatmounts of the carp retina, among 368 cone-connected horizontal cells, which were singly marked by intracellular lucifer yellow under various experimental conditions, 15 cells (4.1%) were found to possess a bifurcate axon and two terminals. the lengths of single and bifurcate axonal processes (an axon plus its terminal) are all comparable, ranging from 400 to 600 micron. | 1985 | 3991048 |
| bile acids. lxxvii. large-scale preparation of 5 alpha-anhydrocyprinol from carp bile. | an improved method of preparation of 5 alpha-anhydrocyprinol from large quantities of carp bile is reported. the following materials were obtained by this method: 5 alpha-anhydrocyprinol, 5 alpha-cholestane-3 alpha,7 alpha,12 alpha,25-tetrol, 5 alpha-cholestane 3 alpha,7 alpha,12 alpha,26-tetrol, an unidentified sterol from the neutral lipid fraction, allocholic acid and allochenodeoxycholic acid from the bile acid fraction. yields of 5 alpha-anhydrocyprinol and bile acids vary in different batc ... | 1985 | 4088981 |
| studies of the heterogeneity of carp liver acid phosphatases: acid phosphatase--i. subunit structure and carbohydrate composition. | the three molecular forms of the carp liver acid phosphatase (acpase) were shown to be dimeric proteins, two of them differing in molecular weights. an activating effect of cona binding on the acpases has been observed. acpase i and acpase ii showed a mol. wt of 122,500 and of 58,884 +/- 3000 for their subunits. it is assumed that acpase i is a sialylated derivative of acpase ii. acpase iii has a mol. wt of 93,132 and the two subunits of 46,556 +/- 4000. a homogeneous acpase i was obtained and i ... | 1985 | 4085217 |
| effects of temperature and transfer from seawater to freshwater on blood microrheology in pacific salmon. | blood of pacific salmon was studied with particular interest in red blood cell (rbc) deformability in relation to migration. blood samples were taken via cardiac puncture or chronic cannula placed in the dorsal aorta and heparinized. as an index of rbc deformability the mean passage time of single rbcs through micropores of 8 micron in diameter and 10 micron in length was determined under a pressure difference of 10 cmh2o. despite about 100 mosmol/l difference in plasma osmolality, there was no ... | 1985 | 4068373 |
| a quantitative study of the nucleus rotundus of the carp. | the present work describes the results of a quantitative study of the nucleus rotundus of c. carpio aimed at determining the volume of the nucleus as a whole and the partial volumes corresponding to the rostral and caudal zones. the overall neuronal density of the nucleus and the density of light and dark cells which forms the neuronal population of the nucleus rotundus were also determined; the light cells were the most represented. most cells were found in the rostral portion while the caudal ... | 1985 | 4067283 |
| temperature dependence of the dielectric properties of blood. | the aim of the work was to investigate the temperature effect in physiological range on the dielectric properties of a fish (cyprinus carpio) blood. by applying an empirical non-damaging method and statistical analysis of the dielectric data, it is observed that a heating of carp blood induces an almost linear value variation of the electrical conductivity and permittivity of the intracellular matter of carp red blood cells. the dielectric data, however, reveal anomalous behaviour for conductivi ... | 1985 | 4043438 |
| effect of thiol-reactive reagents on the permeability of fish erythrocyte membrane for spin-labelled non-electrolytes. | carp erythrocytes were treated with p-chloromercuribenzoate or n-ethylmaleimide. it was observed that these thiol-group inhibitors decreased the transport of spin-labelled hydrophilic compound, 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl, and increased the transport rate of more hydrophobic 2,2,6,6-tetramethylpiperidine-1-oxyl. | 1985 | 4036446 |
| morphometry of muscle fibre types in the carp (cyprinus carpio l.). relationships between structural and contractile characteristics. | ultrastructural parameters of muscle fibre types of the carp (cyprinus carpio l.) were measured and compared with their contractile properties. in red fibres, which are slower than pink fibres, the relative length of the junction between the t system and the sarcoplasmic reticulum (t-sr junction) is smaller and the z lines are thicker than in pink fibres. pink fibres have a smaller relative length of t-sr junction than white fibres from the axial muscles. the two types of red fibres present in c ... | 1985 | 4028116 |
| biochemical compartmentation of fish tissues vii non-specific phosphomonoesterases in vital organs. | the viscera were compartmentalised in relation to non-specific phosphomonoesterase in a major carp, a catfish and a murrel. activity was highest in the middle region of the kidney and lowest in the cephalic region of muscle. the observed variations in various regions of the viscera are discussed in relation to feeding habits, growth rate and biochemical make-up of the viscera. | 1985 | 4013773 |
| spatial orientation of horizontal cell axon terminals in the carp retina. | in flatmounts of the carp (cyprinus carpio) retina, 646 horizontal cells were singly marked by intracellular lucifer yellow ch (ly) in the presence of dopamine or amphetamine, agents which were useful for restricting ly to single injected cells. most axon terminals of cone-connected horizontal cells have a tendency to orient either radially or tangentially in the retinal field with respect to the optic disc as a center. although the fluorescent cellular (dendritic field) area greatly varied depe ... | 1985 | 4075138 |
| the differentiation of germ cells and gonads during development of carp (cyprinus carpio l.). a study with anti-carp sperm monoclonal antibodies. | gonadal development, germ cell differentiation and the appearance of membrane antigenic determinants, specific for male and female germ cells during gonadogenesis, was studied in larval and juvenile carp (cyprinus carpio l.) until 25 weeks after fertilization. indirect immunofluorescence studies with four monoclonal antibodies raised against carp spermatozoa revealed that monoclonal antibody wcs 29 stained the outer membranes of primordial germ cells in larvae from 3 days after fertilization. th ... | 1985 | 2422310 |
| 13c and 113cd nmr studies of the chelation of metal ions by the calcium binding protein parvalbumin. | 13c nmr spectra are presented for the calcium binding protein parvalbumin (pi 4.25) from carp muscle in several different metal bound forms: with ca2+ in both the cd and ef calcium binding sites, with cd2+ in both sites, with 113cd2+ in both sites, and with 113cd2+ in the cd site and lu3+ in the ef site. the different metals differentially shift the 13c nmr resonances of the protein ligands involved in chelation of the metal ion. in addition, direct 13c-113cd spin-spin coupling is observed which ... | 1985 | 3932596 |
| responsiveness and receptive field size of carp horizontal cells are reduced by prolonged darkness and dopamine. | in the fish retina the interplexiform cells contain dopamine and provide a centrifugal pathway from the inner plexiform layer to horizontal cells of the outer plexiform layer. dopamine application reduced the responsiveness and receptive field size of cone horizontal cells, as did a prolonged period of complete darkness. other results suggest that the interplexiform cells may release dopamine after a prolonged period in the dark. the interplexiform-horizontal cell system may modify the strength ... | 1985 | 4035351 |
| light and electron microscopic identification of gonadotrophic cells in the pituitary gland of the goldfish by means of immunocytochemistry. | immunocytochemical techniques were used at the light and electron microscopical levels in order to localize and to characterize the gonadotrophs in the goldfish pituitary gland by means of antibodies to carp gonadotrophin (c-gth) or its subunit (c-gth beta). at the light microscopical level antibodies to c-gth reacted weakly with cells located in the rostral pars distalis (rpd) and strongly with cells of the proximal pars distalis (ppd). the labeling was restricted to the proximal pars distalis ... | 1985 | 4043726 |
| h+ and cl- ion equilibrium across the red cell membrane in the carp. | h2o and electrolyte distribution were studied in carp erythrocytes at various ph values achieved by co2/o2 equilibration in vitro. intracellular ph was measured by means of glass electrode and by dmo-14c. rh+, rcl- and rdmo- varied linearly and in a comparable manner with phe. at phe = 7.8, rh+, rcl- and rdmo- were 0.21, 0.29 and 0.30. rcl- and rdmo- were closely correlated and exhibited only minor differences. rh+ was closely correlated with rcl- and rdmo-, but was, however, significantly lower ... | 1985 | 4048671 |
| a predicted structure of calmodulin suggests an electrostatic basis for its function. | by using interactive computer graphics, two models for calmodulin have been constructed based on the structures of two functionally and structurally related proteins, intestinal calcium-binding protein and carp parvalbumin. the two models have been compared and contrasted to the parent proteins with respect to proportion of solvent-exposed hydrophobic residues, solvent-accessible surface area, and side-chain packing. electrostatic potential surfaces generated for the models suggest a probable bi ... | 1985 | 3860834 |
| occurrence of n-acylethanolamine phospholipids in fish brain and spinal cord. | n-acylethanolamine phospholipids were identified in the central nervous system of the fresh water fish, pike (esox lucius) and carp (cyprinus carpio), at levels ranging from 0.1 to 0.9% of total phospholipid. the n-acylethanolamine phospholipids of carp brain were isolated and characterized by chemical, biochemical and spectroscopic methods. two major species, 1,2-diacyl-sn-glycero-3-phospho(n-acyl)ethanolamines (approx. 30%) and 1-o-(1'-alkenyl)-2-acyl-sn-glycero-3-phospho(n-acyl)ethanolamines ... | 1985 | 4016139 |
| tonotopic organization in the midbrain of a teleost fish. | recordings from small clusters of units within the auditory midbrain of the japanese carp, cyprinus carpio, provide the first evidence for tonotopy within the teleost brain. these findings suggest that place mechanisms of frequency coding and tonotopy, previously thought to have evolved first in amphibians, may be general and ancient features of the vertebrate auditory system. | 1985 | 4027605 |
| the influence of antibiotics on the immune system. ii. modulation of fish leukocyte responses in culture. | we report the immunomodulating effect of two tetracycline analogues (oxytetracycline and doxycycline) on mitogenic and allogeneic stimulation of carp (cyprinus carpio) leukocytes in vitro. both drugs interfered with 3h-thymidine incorporation into the dna of phytohaemagglutinin-stimulated pronephric leukocytes in a dose-dependent manner. low concentrations of oxytetracycline delayed the mitogenic response, but did not reduce it. in addition, several other antibiotics were tested for their possib ... | 1985 | 2930938 |
| electron probe x-ray microanalysis of post-tetanic ca2+ and mg2+ movements across the sarcoplasmic reticulum in situ. | ca2+ and mg2+ movements across the sarcoplasmic reticulum (sr) of frog skeletal muscle fibers were measured in situ by electron probe microanalysis of muscles rapidly frozen following a tetanus. at 400 ms following a 1.2-s tetanus at room temperature, the force had relaxed to base-line, and 0.3 mmol of ca2+/liter of cytoplasmic h2o had been pumped by the sr, indicating that the in situ pumping of the sr ca-atpase is sufficiently high to account for the removal of ca2+ from the ca2+-specific site ... | 1985 | 3158652 |
| reexamination of photoreceptor-bipolar connectivity patterns in carp retina: hrp-em and golgi-em studies. | on- and off-center bipolar cells were identified in the carp retina by means of intracellular recording, intracellular injection of hrp, and golgi silver-chromate impregnation. light and electron microscopy revealed that these functionally different bipolar cells make synaptic contacts with both rods and cones, and that both on- and off-center cells can be further divided into two subtypes (i and ii) according to the relationship between the position of their dendritic processes and the synaptic ... | 1985 | 4056093 |
| uptake and transport of ferritin in the epithelium of carp (cyprinus carpio l.) and the possible immunological implications. | 1985 | 4028172 | |
| kinetic differences at low temperatures between r and t state carbon monoxide-carp hemoglobin. | we use the low-temperature recombination kinetics of carbon monoxide with carp hemoglobin to determine that the r and t states of hemoglobin exhibit different low-temperature geminate recombination kinetics. the peak of the fitted gaussian activation energy spectrum is at 1.5 kcal/mol for r state and 1.8 kcal/mol for t state. the distribution in activation energies is fit well by the agmon-hopfield linear strain model. the t state is fit with a stronger elastic constant than r, and has a larger ... | 1985 | 4016198 |
| studies on the sequence of 2,6-diisopropylnaphthalene metabolite formation using carp hepatocyte. | metabolism of 2,6-diisopropylnaphthalene (2,6-dipn) was studied in freshly isolated carp hepatocytes with special reference to cytochrome p-450-mediated oxidation. the viability of isolated hepatocytes obtained by use of ca2+-free and collagenase-containing hanks buffer was 93%, judging from both trypan blue penetration and lactic dehydrogenase (ldh) leakage. 2,6-dipn was metabolized to form several oxidized products such as the tertiary hydroxy, the primary hydroxy, and two types of dihydroxy d ... | 1985 | 4006835 |
| an example of interaction between environmental pollutants: modification of thiram toxicity to freshwater organisms by nitrites or nitrates in relation to nitrosamine synthesis. | thiram, a dithiocarbamate fungicide, is known to evolve to dimethylnitrosamine (dmna) when associated with nitrites. conditions of appearance of that carcinogenic compound have been studied in short-term experiments by association of the fungicide, nitrates or nitrites, and species representative of freshwater biota. dmna has been estimated by glc equipped with a specific detector. chlorella vulgaris can rapidly produce nitrites from nitrates and dmna is obtained in presence of thiram. daphnia m ... | 1985 | 4006832 |
| the endocrine activity of isolated follicular cells of the carp ovary in primary culture. | oocytes were isolated from the ovaries of carp females of one breed. interstitial tissue was mechanically removed in order to obtain single oocytes surrounded only by the follicular envelope. such a preparation was trypsinized at room temperature and also with greater success at 37 degrees c. the follicular cell suspension contained both cells and cell clumps. it was the mixture of theca (t) and granulosa (g) cells. the temperature of 37 degrees c was preferable for the successful growth of the ... | 1985 | 3859457 |
| fish lymphokines: the production of growth factors in supernatants of mitogen-stimulated pronephros cells of carp (cyprinus carpio). | 1985 | 3161628 | |
| ganglion cells of the terminal nerve: morphology and electrophysiology. | the ganglion cells of the terminal nerve (tn cells) in the carp were identified using intracellular recording and staining techniques. the tn cells showed characteristic location and morphology as well as characteristic responses after electrical shocks to the olfactory nerve and tracts. these features of the tn cells were distinct from those of the mitral or other cells in the olfactory bulb. | 1985 | 4005539 |
| [electrical characteristics of the epithelial tissue and cells of the carp and frog gallbladder]. | transepithelial potential and resistance, the ratio of resistance of the apical to the basal cell membranes (ra/rb) were studied during transmural current clamps in isolated frog and fish gall-bladder (gb) with ringer's solution on both sides. the findings show that: 1) the difference between the mucosal and serosal membrane potentials (mps) was 3-8 mv as determined by transmural potential; 2) the ratio ra/rb depends on the mp value: it was less than 1 if mp was about 30 mv, and more than 1 if m ... | 1985 | 4018298 |
| effect of temperature, 17 alpha-hydroxy-20 beta-dihydroprogesterone and prostaglandin f2 alpha on carp oocyte maturation and ovulation in vitro. | two series of experiments were carried out. in series i, studies were conducted on the effect of 17 alpha-hydroxy-20 beta-dihydroprogesterone (17 alpha 20 beta p; 1 microgram/ml of substrate) on the migration of germinal vesicle (gv) and its breakdown (bd) in the oocytes of carp ovarian fragments, kept at 5, 10, 15, 20, and 25 degrees c. for investigations ovarian fragments were taken both from intact mature females and from those injected with carp hypophysial homogenate (chh) at a dose of 1 mg ... | 1985 | 3858205 |
| temperature acclimation: improved sustained swimming performance in carp at low temperatures. | at low temperatures, the reduction in mechanical power output of the aerobic muscle forces cold-blooded animals, such as carp, to recruit their rapidly fatiguing anaerobic fibers at relatively slow swimming speeds. previous experimental data have suggested that changes in the biochemistry and morphology of the aerobic muscle during cold acclimation might increase its output of mechanical power. the present experiments show that, because of these changes, carp can swim faster at low temperature u ... | 1985 | 17779642 |
| hormonal changes during induced ovulation of the carp, cyprinus carpio. | the changes in plasma concentrations of eight steroids (testosterone, testosterone glucuronide, estradiol, estradiol glucuronide, 17-hydroxyprogesterone, 17,20 beta-dihydroxy-4-pregnen-3-one (17,20 beta p), deoxycorticosterone, and cortisol) have been followed in three individual carp, cyprinus carpio, during ovulation induced by carp pituitary extract. deoxycorticosterone and estradiol glucuronide were not detectable and small amounts of 17,20 beta p were found only in one fish. a priming injec ... | 1985 | 4063009 |
| gill arch movements and the function of the dorsal gill arch muscles in the carp. | the activity coordination of the dorsal gill arch muscles in a teleost, the carp, is described and the effect of their contraction in combination with the respiratory pump movements is analysed. based on their origin and insertion the dorsal branchial arch muscles can be divided into three groups: the external branchial arch levators, connecting the branchial arches to the neurocranium, the internal branchial arch levators, connecting the pharyngobranchials to the neurocranium and the dorsal obl ... | 1985 | 4001606 |
| a comparison of physiological changes in carp, cyprinus carpio, induced by several pollutants at sublethal concentrations. i. the dependency on exposure time. | carp were exposed to 10 different pollutants at sublethal concentrations for 6, 24, or 72 hr. blood, liver, and white muscle samples were taken after the exposure time together with samples of control handled fish. serum concentrations of glucose, cortisol protein, and cholesterol were determined. similarly, the liver and muscle glycogen contents were measured. the effects of the following pollutants were examined: aldrin (100 micrograms/liter), atrazine (100 micrograms/liter), ddt (50 microgram ... | 1985 | 3987597 |
| changes in tension generation and atpase activity in skinned muscle fibres of the carp following temperature acclimation. | common carp (cyprinus carpio l.) were acclimated to either 7 degrees c or 23 degrees c for 1-2 months. skinned fibre preparations were isolated from the white myotomal muscle, and atpase activity measured during maximal isometric contractions. at 7 degrees c, fibres from the cold acclimated fish were found to generate more force than those from warm acclimated fish (123.1 and 97.2kn m-2 respectively), and more "work" (force x time integral) was obtained for each atp hydrolysed. atp turnover per ... | 1985 | 3160006 |
| l-glutamate depolarizes on-off transient type of amacrine cells in the carp retina: an ionophoretic study. | amacrine cells generating the on-off transient type of photoresponse, which is most frequently encountered, were identified by intracellular recording and staining with a fluorescent dye lucifer yellow in the carp (cyprinus carpio) retina. l-glutamate applied ionophoretically slowly depolarized the amacrine cells by about 10 mv and reduced their photoresponses during the application. such changes gradually recovered following the cessation of l-glutamate injection. the results indicate that this ... | 1985 | 2858255 |
| telencephalic afferent nuclei in the carp diencephalon, with special reference to fiber connections of the nucleus preglomerulosus pars lateralis. | fiber connections of the nucleus preglomerulosus pars lateralis (pgl), which primarily provides afferent inputs to the telencephalon, were examined in carp by means of horseradish peroxidase tracing methods. the major afferent sources of pgl are the bilateral nucleus tuberis anterior and a few projections are found deriving from the ipsilateral nucleus ventromedialis thalami, nucleus centralis posterior, dorsal periventricular hypothalamus, and torus semicircularis. axons arising in the pgl can ... | 1986 | 2429725 |
| gap-junctional contacts of luminosity-type horizontal cells in the carp retina: a novel pathway of signal conduction from the cell body to the axon terminal. | l-type horizontal cells (271) of the carp retina were studied with the aid of intracellular injections of lucifer yellow and horseradish peroxidase (hrp). coupling among these cells was revealed by passage of the dye out of the injected cell. thick processes that crossed axons filled with lucifer yellow also contained the stain. these thick processes appear to be the axon terminals of other horizontal cells. serial reconstruction of an axon terminal by electron microscopy revealed two groups of ... | 1986 | 3734163 |
| the time-course of appearance and net accumulation of horseradish peroxidase (hrp) presented orally to juvenile carp cyprinus carpio (l.). | a sensitive enzyme-linked immunosorbent assay (elisa) technique was used in order to determine horseradish peroxidase (hrp) uptake from the carp gut. hrp was detected in blood plasma and various tissues within 15 min of oral intubation. the time-course of net accumulation (uptake-degradation) over a 2 hr period was recorded. the presence of hrp reached a maximum in the body tissues approximately 1 hr after intubation and on a microgram/g wet weight basis the order of accumulation within the tiss ... | 1986 | 2875835 |
| internally transposed signal sequence of carp preproinsulin retains its functions with the signal recognition particle. | it is shown that the signal sequence of carp preproinsulin is functional with the dog pancreatic signal recognition particle (srp) both when present at its normal location at the amino-terminus of the protein or when engineered to an internal location. inhibition of translation by srp in the absence of microsomal membranes, reconstitution by srp of the translocation competence of high-salt inactivated microsomes and signal peptide cleavage all occur with the signal sequence being preceded by a h ... | 1986 | 3000822 |
| a signal sequence is required for the functions of the signal recognition particle. | it is shown that removal of the signal sequence of carp preproinsulin by gene technology abolishes the interaction between the polypeptide chain and the signal recognition particle with respect to both its functions: the inhibition of translation by the signal recognition particle in the absence of microsomal membranes, and the reconstitution of the protein translocation competence of high-salt inactivated microsomes. it is concluded that a signal sequence is absolutely required for the function ... | 1986 | 3004463 |
| complete nucleotide sequence of the matrix protein mrna of vesicular stomatitis virus (new jersey serotype). | the complete nucleotide sequence of the mrna of the matrix (m) protein of vesicular stomatitis virus [new jersey serotype, vsv(nj)] was derived from a cdna clone and mrna. the mrna is 758 nucleotides long (excluding polyadenylic acid) and encodes a protein of 229 amino acids. the predicted amino acid sequence was compared with that of the corresponding protein of indiana serotype [vsv(ind)] and a fish rhabdovirus, spring viremia of carp virus (svcv). an amino acid identity of 62% was found betwe ... | 1986 | 3006343 |