Publications

[skin prick test using two different allergen prick reagents, a comparative study in shanghai].	to compare the results of skin prick test with allergens of allergopharma company or alk company in shanghai area.	2014	25185289
[efficacy of individualized sublingual immunotherapy with dermatophagoides farinae drops on patients with allergic rhinitis of different age groups].	to evaluate the efficacy of personal sublingual immunotherapy (slit) with dermatophagoides to study the efficacy of dermatophagoides farinae drops for allergic rhinitis (ar) of different age groups.	2014	25185278
efficacy and safety of house dust mite sublingual immunotherapy in monosensitized and polysensitized children with respiratory allergic diseases.	the efficacy of single-allergen-specific immunotherapy in polysensitized subjects is a matter of debate. this study aimed to investigate the efficacy of house dust mite (hdm) sublingual immunotherapy (slit) in monosensitized and polysensitized children.	2014	25145986
gene expression in the skin of dogs sensitized to the house dust mite dermatophagoides farinae.	atopic dermatitis is a multifactorial allergic skin disease in humans and dogs. genetic predisposition, immunologic hyperreactivity, a defective skin barrier, and environmental factors play a role in its pathogenesis. the aim of this study was to analyze gene expression in the skin of dogs sensitized to house dust mite antigens. skin biopsy samples were collected from six sensitized and six nonsensitized beagle dogs before and 6 hr and 24 hr after challenge using skin patches with allergen or sa ...	2014	25098772
house dust mite sublingual immunotherapy is safe and appears to be effective in moderate, persistent asthma.	the efficacy and safety of sublingual immunotherapy in house dust mite-induced asthma have yet to be firmly established. we report the results of a double-blind, placebo-controlled, randomized clinical trial performed in mainland china.	2014	25056584
salvia plebeia suppresses atopic dermatitis-like skin lesions.	salvia plebeia r. br. (lamiaceae) has been used for folk medicines in asian countries, including korea and china, to treat skin inflammatory diseases and asthma. in this study, we investigated the effects of s. plebeia extract (spe) on atopic dermatitis (ad)-like skin lesions and defined underlying mechanisms of action. we established an ad model in balb/c mice by repeated local exposure of house dust mite extract (dermatophagoides farinae extract, dfe) and 2,4-dinitrochlorobenzene (dncb) to the ...	2014	25004886
[effect of immunotherapy with recombinant allergen group 3 from dermatophagoides farinae in asthma mice].	to explore the effect of specific immunotherapy with major 3 group recombinant allergen rder f 3 of dermatophagoides farinae in murine asthma model.	2014	25518591
[experimental study on the der f 1 mrna molecules derived from dermatophagoides farinae for specific immunotherapy on murine model of asthma].	to investigate the effect of der f 1 mrna molecules for specific immunotherapy on murine model of asthma.	2014	25518589
increased il-22 level in allergic rhinitis significantly correlates with clinical severity.	il-22 regulates various processes and has been linked to diverse effects. however, the importance of il-22 in the pathogenesis of allergic rhinitis (ar) remains poorly understood. this study sought to evaluate the levels of il-22 and il-17a in ar patients and their association with clinical severity ofn ar.	2014	25514475
group 1 allergen genes in two species of house dust mites, dermatophagoides farinae and d. pteronyssinus (acari: pyroglyphidae): direct sequencing, characterization and polymorphism.	group 1 allergens of dermatophagoides farinae (der f 1) and d. pteronyssinus (der p 1) dominate overall allergic responses in house dust mite allergy patients. the need for accurate identification and characterization of representative variants of group 1 allergens in any given geographic locality has been emphasized for development of appropriate allergen extracts. regional amino acid sequence polymorphism has been described but the extent of this polymorphism is not well understood. such data ...	2014	25494056
[choosing the best panel of aeroallergen prick-tests for patients in lebanon with rhinitis and asthma: a retrospective study of 2350 patients].	the aim of this study was to evaluate the profile of skin prick-test reactivity to different aeroallergens in patients with rhinitis and asthma in lebanon and its geographic variation within the country. it was also to suggest a minimal panel of allergens that should be used to detect sensitized patients.	2014	25433589
food allergy enhances allergic asthma in mice.	atopic march refers to the typical transition from a food allergy in early childhood to allergic asthma in older children and adults. however the precise interplay of events involving gut, skin and pulmonary inflammation in this process is not completely understood.	2014	25433406
the leaves of broussonetia kazinoki siebold inhibit atopic dermatitis-like response on mite allergen-treated nc/nga mice.	broussonetia kazinoki siebold. (b. kazinoki) has long been used in the manufacture of paper in asian countries. although b. kazinoki leaves (bk) have been employed in dermatological therapy, use of bk has not been tested in patients with atopic dermatitis (ad). using nc/nga mice, which are genetically predisposed to develop ad-like skin lesions, we confirmed the efficacy of bk in ad treatment. bk extract was applied topically to dermatophagoides farinae-induced ad-like lesions in nc/nga mice, an ...	2014	25414775
influence of asian dust particles on immune adjuvant effects and airway inflammation in asthma model mice.	an asian dust storm (ads) contains airborne particles that affect conditions such as asthma, but the mechanism of exacerbation is unclear. the objective of this study was to compare immune adjuvant effects and airway inflammation induced by airborne particles collected on ads days and the original ads soil (cj-1 soil) in asthma model mice.	2014	25386753
the serine protease inhibitor, 4-(2-aminoethyl) benzene sulfonyl fluoride hydrochloride, reduces allergic inflammation in a house dust mite allergic rhinitis mouse model.	serine protease inhibitors are involved in immune development, anti-inflammatory mechanisms, and tissue repair. in the present study, the serine protease inhibitor 4-(2-aminoethyl) benzene sulfonyl fluoride hydrochloride (aebsf) was evaluated for its prophylactic and therapeutic applications in a mouse model of allergic rhinitis (ar).	2014	25374756
a dna vaccine encoding a chimeric allergen derived from major group 1 allergens of dust mite can be used for specific immunotherapy.	immunization with dna-based constructs has been shown to be against the antigen and the response is skewed in such a way as to ameliorate the symptoms of allergic disease. this approach is particularly useful in the treatment of allergic inflammatory diseases, such as asthma. the major group 1 allergen from house dust mites is one of the triggers of allergic asthma. this study explores whether a chimeric gene r8, derived from the major group 1 allergen of house dust mite species (dermatophagoide ...	2014	25337189
[effects of sublingual immunotherapy on serum il-17 and il-35 levels in children with allergic rhinitis or asthma].	to study the clinical effect and mechanisms of specific sublingual immunotherapy (slit) for the treatment of allergic rhinitis or asthma in children.	2014	25523566
damp molecules s100a9 and s100a8 activated by il-17a and house-dust mites are increased in atopic dermatitis.	s100a9 and s100a8 are called damage-associated molecular pattern (damp) molecules because of their pro-inflammatory properties. few studies have evaluated s100a9 and s100a8 function as damp molecules in atopic dermatitis (ad). we investigated how house-dust mites affect s100a9 and s100a8 expression in th2 cytokine- and th17 cytokine-treated keratinocytes, and how secretion of these molecules affects keratinocyte-derived cytokines. finally, we evaluated expression of these damp molecules in ad pa ...	2014	25308296
oxidative stress status in umbilical cord blood from neonates born to mothers with atopic asthma.	our objective was to assess the oxidative stress status and analyse the relationship between an oxidant/antioxidant imbalance and the mediator release properties of cord blood basophils from neonates born to mothers with atopic asthma.	2014	23735076
the efficacy of sublingual immunotherapy with dermatophagoides farinae vaccine in a murine atopic dermatitis model.	sublingual immunotherapy (slit) is a potential treatment for house dust mite-induced atopic dermatitis (ad). however, the mechanisms of action are not clear.	2015	25258013
allergy to dust mites may contribute to early onset and severity of alopecia areata.	a higher risk of allergic diseases such as rhinitis, asthma and atopic eczema (atopic dermatitis) has been reported for patients with alopecia areata (aa) compared with the general population, but the significance of this is still largely unclear.	2015	25252126
human umbilical cord blood mesenchymal stem cell-derived pge2 and tgf-β1 alleviate atopic dermatitis by reducing mast cell degranulation.	mesenchymal stem cell (msc) is a promising tool for the therapy of immune disorders. however, their efficacy and mechanisms in treating allergic skin disorders are less verified. we sought to investigate the therapeutic efficacy of human umbilical cord blood-derived mscs (hucb-mscs) against murine atopic dermatitis (ad) and to explore distinct mechanisms that regulate their efficacy. ad was induced in mice by the topical application of dermatophagoides farinae. naïve or activated-hucb-mscs were ...	2015	25522163
luffa cylindrica suppresses development of dermatophagoides farinae-induced atopic dermatitis-like skin lesions in nc/nga mice.	the fruit pulp of luffa cylindrica roemer (cucurbitaceae) (lc) has been used to induce hemostasis, resolve phlegm and clear fever in traditional korean medicine. however, the efficacy of lc has not been examined in atopic dermatitis (ad).	2015	25327534
the draft genome, transcriptome, and microbiome of dermatophagoides farinae reveal a broad spectrum of dust mite allergens.	a sequenced house dust mite (hdm) genome would advance our understanding of hdm allergens, a common cause of human allergies.	2015	25445830
in vitro evaluation of allergen potencies of commercial house dust mite sublingual immunotherapy reagents.	the clinical efficacy of allergen-immunotherapy is known to be dose dependent. however, optimal maintenance dosage has not yet been determined for sublingual immunotherapy (slit). furthermore, since companies adopt their own units for expression of allergenicity, the allergen concentrations of individual reagents cannot be compared easily. we sought to measure and compare the allergenicities of 3 commercially available house dust mite (hdm) slit regents and a subcutaneous immunotherapy reagent.	2015	25729619
three-year follow-up results of sublingual immunotherapy in patients with allergic rhinitis sensitized to house dust mites.	this study investigated the long-term efficacy, safety, and compliance associated with sublingual immunotherapy (slit) in korean patients with allergic rhinitis sensitized to house dust mites.	2015	25729618
belching, regurgitation, chest tightness and dyspnea: not gastroesophageal reflux disease but asthma.	belching is a common symptom of gastroesophageal reflux disease. if the symptoms are not relieved after anti-reflux treatment, another etiology should be considered. here, we report a case of a 43-year-old man who presented with belching, regurgitation, chest tightness and dyspnea for 18 mo, which became gradually more severe. gastroscopic examination suggested superficial gastritis. twenty-four-hour esophageal ph monitoring showed that the demeester score was 11.4, in the normal range. high-res ...	2015	25663791
asian sand dust aggregate causes atopic dermatitis-like symptoms in nc/nga mice.	asian sand dust (asd) originates from the arid and semiarid areas of china, and epidemiologic studies have shown that asd exposure is associated with various allergic and respiratory symptoms. however, few studies have been performed to assess the relationship between skin inflammation and asd exposure.	2015	25642251
reproductive biology of euroglyphus maynei with comparisons to dermatophagoides farinae and d. pteronyssinus.	the reproductive biology of the house dust mite, euroglyphus maynei, is not well studied. this mite is usually less common in homes than dermatophagoides farinae and d. pteronyssinus. when it is present, it usually co-inhabits with the dermatophagoides spp. and is more restricted in geographical distribution. in this study, the duration of the life cycle (egg to adult) at 23 and 30 °c at 75% relative humidity (rh) and fecundity at 23 °c and 75% rh were determined for e. maynei and the data were ...	2015	25633266
in silico structural analysis of group 3, 6 and 9 allergens from dermatophagoides farinae.	dermatophagoides farinae (hughes; acari: pyroglyphidae) are the predominant source of dust mite allergens, which provoke allergic diseases, such as rhinitis, asthma and eczema. of the 30 allergen groups produced by d. farinae, the der f 3, der f 6 and der f 9 allergens are all trypsin‑associated proteins, however little else is currently known about them. the present study used in silico tools to compare the amino acid sequences, and predict the secondary and tertiary structures of der f 3, der ...	2015	25572027
[the impact of subcutaneous immunotherapy with dermatophagoides farinae and dermatophagoides pteronyssinus on the quality of life of patients with allergic rhinitis and asthma].	the prevalence of asthma and allergic rhinitis in colombia is increasing at the same rate as it is in other parts of the world. it has been determined that allergen-specific subcutaneous immunotherapy is effective in subjects with allergic rhinitis and asthma that are sensitized to house dust mites: dermatophagoides farinae and dermatophagoides pteronyssinus .	2015	24967933
toxicity of hiba oil constituents and spray formulations to american house dust mites and copra mites.	dermatophagoides farinae and tyrophagus putrescentiae are recognised as an important source of allergens. an assessment was made of the toxicity of hiba, thujopsis dolabrata var. hondai, oil and 13 organic compounds and the control efficacy of four experimental spray formulations containing the oil (5-30 g l(-1) spray) against both mite species.	2015	24916027
[frequency of sensitization to mites, cockroach and shrimp in adults with respiratory allergy].	sensitization to mites and cockroach are a common cause of respiratory allergy, especially in children. there is little information about its cross-reactivity with shrimp, which is common in adult patients.	2015	24915617
[sensitization to house dust and storage mites in allergic adults from the south of mexico city].	mites are the most common cause of respiratory allergy. sensitization to house dust mites is estimated at 30%. families euroglyphus and dermatophagoides, are the most important. recently, storage mites, from the families acaridae and glyciphagidae, have become more important as a cause of allergic respiratory diseases.	2015	24008067
characterization of arginine kinase, anovel allergen of dermatophagoides farinae (der f 20).	to characterize a novel allergen, the dermatophagoides farinae-derived arginine kinase (der f 20).	2015	26885278
dose-dependent immunological responses after a 6-month course of sublingual house dust mite immunotherapy in patients with allergic rhinitis.	house dust mite (hdm) immunotherapy has proven efficacy in treating allergic rhinitis (ar) symptoms. this trial evaluated the dose-response relationship of slitoneultra® hdm mix based on immunological parameters and safety in subjects with moderate-to-severe hdm ar not controlled by symptomatic medication.	2015	26789997
[clinical efficacy and safety of rush immunotherapy in patients with allergic rhinitis].	to evaluate the efficacy and safety of rush immunotherapy (rit) for patients with persistent allergic rhinitis (ar).	2015	26696201
[effects of house dust mite sublingual immunotherapy in children with allergic rhinitis and asthma].	to evaluate the efficacy of slit with dermatophagoides farinae drops in children with allergic rhinitis and allergic asthma according to its different duration.	2015	26696198
[onset time and efficacy of sublingual immunotherapy with dermatophagoides farinae drops in children with allergic rhinitis].	to investigate the onset time and efficacy of sublingual immunotherapy (slit) with dermatophagoides farinae drops in children with house dust mites (hdm)-induced allergic rhinitis (ar).	2015	26696197
[factors associated with the presence of allergen-specific ige responses in asthma patients who had no ige responses detectable by mast-26].	to elucidate the characteristics of patients with asthma who have specific ige responses to inhaled allergens detected by immunocap, which is not detectable by mast-26.	2015	26657911
characterization of a new subtype of allergen in dermatophagoides farinae-der f 28.	house dust mites (hdms) are the major sources of indoor allergens which induce asthma, dermatitis, rhinitis, and some other allergic diseases. close to 30 sub-allergens have been identified.	2015	26623108
pharmacodynamic and pharmacokinetic evaluation of house dust mite sublingually administered immunotherapy tablet in the treatment of asthma.	sublingual immunotherapy (slit) for house dust mite (hdm) allergy has an evidence of efficacy demonstrated by meta-analyses, but marked differences are reported between the various slit products. the standardized quality (sq) hdm sublingual tablet containing dermatophagoides pteronyssinus and dermatophagoides farinae in a 1:1 ratio (mk-8237) is a qualitative advance for slit products.	2015	26565665
allergen stabilities and compatibilities in immunotherapy mixtures that contain cat, dog, dust mite, and cockroach extracts.	indoor allergen mixtures that contain cat, dog, dust mite, and cockroach extracts are commonly used in allergy clinics for subcutaneous immunotherapy, but product-specific stabilities and mixing compatibilities in these complex patient formulas have not been determined.	2015	26522255
[the effect research of specific immunotherapy of allergic rhinitis and allergic rhinitis combined bronchial asthma].	to study the therapeutic effects of the specific immunotherapy (sit) on allergic rhinitis and allergic rhinitis combined bronchial asthma. methed: all patients were classified into allergic rhinitis group (ar group) with 32 patients and allergic rhinitis combined bronchial asthma group (ar+ba group) with 32 patients. another health control group with 32 cases was designed as well. the allergens,symptom scores and therapeutic effects of the former two-group patients were analysis, and the serums ...	2015	26513998
the potential for a blood test for scabies.	scabies afflicts millions of people worldwide, but it is very difficult to diagnose by the usual skin scrape test, and a presumptive diagnosis is often made based on clinical signs such as rash and intense itch. a sensitive and specific blood test to detect scabies would allow a physician to quickly make a correct diagnosis.	2015	26492406
[positive allergens in children with different allergic diseases].	to investigate the major allergens in children with different allergic diseases, and to provide theoretical evidence for the clinical prevention, diagnosis, and treatment of allergic diseases in children.	2015	26412169
chlorella vulgaris attenuates dermatophagoides farinae-induced atopic dermatitis-like symptoms in nc/nga mice.	atopic dermatitis (ad) is a chronic and inflammatory skin disease that can place a significant burden on quality of life for patients. ad most frequently appears under the age of six and although its prevalence is increasing worldwide, therapeutic treatment options are limited. chlorella vulgaris (cv) is a species of the freshwater green algae genus chlorella, and has been reported to modulate allergy-inducible factors when ingested. here, we examined the effect of cv supplementation on ad-like ...	2015	26404252
repetitive immunoassay with a surface acoustic wave device and a highly stable protein monolayer for on-site monitoring of airborne dust mite allergens.	this work describes a sensor to be incorporated into the on-site monitoring system of airborne house dust mite (hdm) allergens. a surface acoustic wave (saw) device was combined with self-assembled monolayers of a highly stable antibody capture protein on the saw surface that have high resistance to ph change. a sandwich assay was used to measure a hdm allergen, der f 1 derived from dermatophagoides farinae. capture antibodies were cross-linked to a protein g based capture layer (orla85) on the ...	2015	26378678
efficacy evaluation of a pollen blocker cream against dust-mite allergy: a multicenter, randomized, double-blind, placebo-controlled crossover trial.	to further evaluate the efficacy and safety of a pollen blocker cream against dust-mite allergy.	2015	26358336
characterization of der f 29, a new allergen from dermatophagoides farinae.	more than 30 allergens have been identified from dermatophagoides farina (d. farina), which is one of the main species of house dust mites. the mite allergens are an important factor contributing to allergic disease in the world. as the detection and identification of new allergens is critical for the diagnosis or treatment of allergic diseases, we sought to characterize the profilin of d. farina (der f 29) in this study. the results showed that 21% of allergic patients displayed positive result ...	2015	26328014
expression, purification and characterization of der f 27, a new allergen from dermatophagoides farinae.	the house dust mite (hdm), dermatophagoidesfarinae (d. farina), is one of the most important indoor allergen sources and a major elicitor of allergic asthma; itscharacterization is important in the diagnosis and immunotherapy of mite allergen-relevant diseases. this study aims to characterize a novel allergen, the d. farinae-derived serpin (der f 27). in this study, the total rna of d. farinae was extracted, and the der f 27 gene was cloned and expressed. the allergenicity of recombinant der f 2 ...	2015	26328010
berberine reduce allergic inflammation in a house dust mite allergic rhinitis mouse model.	berberine (ber), used widely as an antibacterial, antifungal, and anti-inflammatory drug, has long been used as a gastrointestinal remedy in chinese traditional medicine. recent reports have suggested that ber suppresses th17 responses that was mediated by direct actions on t cells and thymic stromal lymphopoietin production in primary mast cells. it has been suggested that ber may be useful in treating allergic response. the purpose of this study was to assess the effects of ber treatment on al ...	2015	26275466
[percentage of th17 cells in spleen and il-17 level in bronchoalveolar lavage fluid in dermatophagoides farinae allergic asthma mice].	to detect the percentage of th17 cells in spleen and il-17 level in bronchoalveolar lavage fluid in dermatophagoides farinae allergic asthma mice.	2015	26245125
effect of high-dose sublingual immunotherapy on respiratory infections in children allergic to house dust mite.	allergic rhinitis is characterized by eosinophil inflammation. allergic inflammation may induce susceptibility to respiratory infections (ri). house dust mite (hdm) sensitization is very frequent in childhood. allergen immunotherapy may cure allergy as it restores a physiologic immune and clinical tolerance to allergen and exerts anti-inflammatory activity.	2015	26240793
clinical characteristics, treatments, and prognosis of atopic eczema in the elderly.	atopic eczema (ae) in the elderly is gradually increasing and has been added to the classification of ae in recent years. this investigation retrospectively analyzed 60 patients with elderly ae. among the clinical characteristics, a male predominance, existence of several patterns of onset and clinical course, and associations with immunoglobulin (ig)e-allergic-status and asthmatic complication were observed. the highest positive-rate and positive-score for serum-specific ige against dermatophag ...	2015	26239460
vaccination with different doses of dermatophagoides farinae in a mouse model of allergic asthma.	the efficacy of vaccination with different doses of a vaccine has not been clarified. the purpose of this study was to investigate the relationship between the doses of purified house dust mite allergen, and allergic airway inflammation and airway reactivity in a mouse model of allergic asthma.	2015	26226406
sick building syndrome (sbs) among office workers in a malaysian university--associations with atopy, fractional exhaled nitric oxide (feno) and the office environment.	there are few studies on sick building syndrome (sbs) including clinical measurements for atopy and fractional exhaled nitric oxide (feno). our aim was to study associations between sbs symptoms, selected personal factors, office characteristics and indoor office exposures among office workers from a university in malaysia. health data were collected by a questionnaire (n=695), skin prick test (spt) (n=463) and feno test (n=460). office settled dust was vacuumed and analyzed for endotoxin, (1,3) ...	2015	26225741
[immunotherapy effect of ploypeptide vaccine of allergen group 1 from dermatophagoides farinae on asthma mice].	to investigate the enzymatic hydrolysates of proder f land their hydrolytic products for specific immunotherapy.	2015	26930933
[study on the efficacy and safety of sublingual immunotherapy with standardized dermatophagoides farinae drops for allergic rhinitis].	to evaluate the efficacy and safety of the sublingual immunotherapy with dermatophagoides farinae drops on patients with allergic rhinitis.	2015	26201187
variation in allergen content in sublingual allergen immunotherapy with house dust mites.	allergen immunotherapy is a treatment modality which can be applied using different vaccines. the aim of this study was to quantify and compare the allergen content of different house dust mites (hdm)' sublingual treatments and to review the evidence on their efficacy.	2015	26185961
structural analysis of der p 1-antibody complexes and comparison with complexes of proteins or peptides with monoclonal antibodies.	der p 1 is a major allergen from the house dust mite, dermatophagoides pteronyssinus, that belongs to the papain-like cysteine protease family. to investigate the antigenic determinants of der p 1, we determined two crystal structures of der p 1 in complex with the fab fragments of mabs 5h8 or 10b9. epitopes for these two der p 1-specific abs are located in different, nonoverlapping parts of the der p 1 molecule. nevertheless, surface area and identity of the amino acid residues involved in hydr ...	2015	26026055
[efficacy of sublingual immunotherapy with dermatophagoides farinae drops on patients with allergic rhinitis of different symptom severity].	to evaluate the efficacy of sublingual immunotherapy (slit) with dermatophagoides farinae drops for allergic rhinitis (ar) of different symptom severity.	2015	26012292
efficacy of sublingual immunotherapy with dermatophagoides farinae extract in monosensitized and polysensitized patients with allergic rhinitis: clinical observation and analysis.	to investigate differences in the efficacy of sublingual immunotherapy with dermatophagoides farinae drops in monosensitized and polysensitized allergic rhinitis patients.	2015	26000283
house dust mite allergen regulates constitutive apoptosis of normal and asthmatic neutrophils via toll-like receptor 4.	house dust mites (hdms) induce allergic diseases such as asthma. neutrophil apoptosis is an important process of innate immunity, and its dysregulation is associated with asthma. in this study, we examined the effects of hdm on constitutive apoptosis of normal and asthmatic neutrophils. extract of dermatophagoides pteronissinus (dp) inhibited neutrophil apoptosis, but dermatophagoides farinae extract had no effect. anti-apoptotic signaling mediated by dp involves in tlr4, lyn, pi3k, akt, erk, an ...	2015	25973752
efficacies of immunotherapy with polypeptide vaccine from proder f 1 in asthmatic mice.	allergic asthma is associated with the major house dust mite group 1 allergens der p 1 and der f 1, which belongs to the papin-like protease family and is the most potent of indoor allergens and allergen-specific immunotherapy (sit), is seen as effective intervention for the entity. the current study was designed to verify the sit efficacies of the enzymatic hydrolysates (papain and trypsin) in mice with asthma. we initially developed the asthmatic mouse models with proder f 1, and respectively ...	2015	25932130
population growth and allergen content of cultured euroglyphus maynei house dust mites.	the house dust mite, euroglyphus maynei, occurs in homes worldwide and is an important source of many allergens. many patients sensitive to dermatophagoides farinae and d. pteronyssinus are also sensitive to e. maynei. extracts to detect sensitivity to e. maynei and reagents to detect e. maynei allergens in the environment or in cultures are not readily available. information for the culture of e. maynei and for the determination of allergen and endotoxin levels in cultures is limited.	2015	25924978
asthma, airway symptoms and rhinitis in office workers in malaysia: associations with house dust mite (hdm) allergy, cat allergy and levels of house dust mite allergens in office dust.	a prevalence study was conducted among office workers in malaysia (n= 695). the aim of this study was to examine associations between asthma, airway symptoms, rhinitis and house dust mites (hdm) and cat allergy and hdm levels in office dust. medical data was collected by a questionnaire. skin prick tests were performed for hdm allergens (dermatophagoides pteronyssinus, dermatophagoides farinae) and cat allergen felis domesticus. indoor temperature and relative air humidity (rh) were measured in ...	2015	25923543
phase ii/iii clinical trial to assess the tolerability and immunological effect of a new updosing phase of dermatophagoides mix-based immunotherapy.	immunologically enhanced subcutaneous specific immunotherapy (scit) has been developed with a fast and simplified updosing phase containing equal parts of the house dust mites (hdm) dermatophagoides pteronyssinus and dermatophagoides farinae (dermatophagoides mix) adsorbed on aluminum hydroxide.	2015	25898693
early-life house dust mite allergens, childhood mite sensitization, and respiratory outcomes.	exposure to indoor allergens during early life may play a role in the development of the immune system and inception of asthma.	2015	25858551
proteomic and immunological identification of two new allergens from silkworm (bombyx mori l.) pupae.	this study explored food allergy caused by eating silkworm (bombyx mori l.) pupae, a traditionally accepted food and animal feed in east and southeast asia, and identified two new allergens by proteomic and immunological methods. proteins isolated from silkworm pupae were separated by two-dimensional gel electrophoresis (2-de); pooled sera from patients allergic to silkworm pupa proteins were used to detect immunoglobulin e (ige)-binding proteins by western blotting, and allergens specific for s ...	2015	26155181
studies on the occurrence, identification and control of house dust mites at rural houses of shebin el-kom locality, egypt.	the present study was conducted at elkom elakhdar village, shebin el-kom, menoufia governorate along 2012 year seasons, to calculate and identify the species composition and the occurrence frequency of the extracted dust mites collected from three building ages at rural houses, as well as to determine the toxicity limits of different concentrations of three plant essential oils against two species of the family pyroglyphidae the main causal of allergy to humans. the obtained results revealed tha ...	2015	26506648
the clinical relevance of various hypersensitivity tests in patients with atopic dermatitis as assessed by their history, scorad changes, and number of days with need of anti-inflammatory treatment.	immediate and delayed hypersensitivity reactions can play a role in the pathogenesis of atopic dermatitis (ad). we tested 71 patients (median age 5 years) with ad for hypersensitivity to grass and birch pollen, dermatophagoides pteronyssinus, and dermatophagoides farinae using atopy patch test (apt), skin prick test (spt), and specific ige measurement. the sensitivity (se) and specificity (sp) of the tests were calculated on the basis of personal history of ad exacerbation, clinical ad score (sc ...	2015	26155366
correlations between allergen-specific ige serum levels in patients with ocular allergy.	to evaluate ocular allergies in patients at the hospital do servidor publico estadual de sao paulo (hspe) and the correlations with serum allergen-specific immunoglobulin e levels.	2015	26165789
induction of muc5ac mucin expression by histamine through the activation of its core promoter region.	this study provides evidence that histamine induced muc5ac mrna expression through the activation of the core region of its promoter. it may also help in approaching new therapeutic strategies in airway mucins hypersecretory diseases.	2015	25872410
solanum tuberosum l. cv jayoung epidermis extract inhibits mite antigen-induced atopic dermatitis in nc/nga mice by regulating the th1/th2 balance and expression of filaggrin.	solanum tuberosum l. cv jayoung (jy) is a potato with dark purple flesh and contains substantial amounts of polyphenols. in this study, we evaluated the therapeutic effects of s. tuberosum l. cv jy in a mouse model of dermatophagoides farinae body (dfb)-induced atopic dermatitis (ad). the ethanol extract of the peel of jy (epj) ameliorated dfb-induced dermatitis severity, serum levels of immunoglobulin e (ige) and thymus and activation-regulated chemokine. histological analysis of the skin also ...	2015	26102094
sero-prevalence study of ige responses to allergens from malaysian house dust (hdm) and storage mites (sm).	allergens of dermatophagoides and blomia species are well-characterized but not for other species. this study was conducted to determine the prevalence of allergic sensitization to house dust (hdm) and storage mites (sm). one hundred adult subjects (aged ≥ 18) were recruited. the mite specific ige of all allergic subjects were higher compared with healthy subjetcs despite being not statistically significant except for d. farinae and g. malaysiensis. the mean serum ige levels against hdm and sm f ...	2015	26695214
[sensitization to blomia tropicalis among asthmatic patients in yaoundé, cameroon].	the aim of this study was to determine the prevalence and investigate associated factors for blomia tropicalis (bt) sensitization in adolescent and adult patients with asthma in yaoundé (cameroon).	2015	25618201
[sensitisation profile to airborne allergens of patients followed for asthma in cotonou, benin. a cross-sectional study using prick-tests].	the sensitisation profile to airborne allergens of asthma patients followed in benin is not known.	2015	25480388
[identification of house dust mite fauna from allergic patients' mattress in six mexican states].	house dust is considered an important source of aeroallergens, and the mites living there, are the main cause of allergy to it. in méxico, there have been few about house dust fauna, and mostly limited to méxico city.	2015	24274602
immunopotentiator from pantoea agglomerans prevents atopic dermatitis induced by dermatophagoides farinae extract in nc/nga mouse.	pantoea agglomerans lps (immunopotentiator from pantoea agglomerans 1: ip-pa1) has been reported to have anti-inflammatory effects in in vitro and in vivo models. the aim of the present study was to investigate the effects of orally-administered ip-pa1 on atopic dermatitis (ad) symptoms induced by dermatophagoides farinae body extract (dfe) in nc/nga mice.	2015	26168493
sensitization rates for various allergens in children with allergic rhinitis in qingdao, china.	the aim of this study was to determine the prevalence of sensitization to common allergens in children with allergic rhinitis (ar) living in qingdao, china. we conducted a retrospective analysis for ar cases, who underwent skin prick tests (spt) in qingdao. a total of 2841 children with ar qualified for the inclusion criteria (age 3-5 years: 1500 children; age 6-12 years: 1341 children). the most common inhaled allergens to which the ar children were sensitive were dermatophagoides pteronyssinus ...	2015	26371014
toxoplasma gondii infection suppresses house dust mite extract-induced atopic dermatitis in nc/nga mice.	toxoplasma gondii (t. gondii) is an obligate intracellular protozoan parasite that infects humans and animals via congenital or postnatal routes, and it is found worldwide. modulation of the immune system by parasite infection is proposed to suppress allergic inflammation. growing evidences have shown that interleukin (il)-10-producing regulatory b cells (b(regs)) and cd4+cd25+foxp3+ regulatory t cells (t(regs)) induced by parasite infection play a critical role in allergic or autoimmune disease ...	2015	26333702
expression and refolding of mite allergen pro-der f1 from inclusion bodies in escherichia coli.	house dust mite (dermatophagoides farinae) allergen der f1 is one of the most important indoor allergens associated with asthma, eczema and allergic rhinitis in humans. therefore, sufficient quantities of der f1 cysteine protease to be used for both experimental and therapeutic purposes are very much needed. using recombinant dna technology, high expression rates of cysteine proteases were obtained. the cdna sequence encoding pro-der f1 was cloned and expressed in escherichia coli using the t7 b ...	2015	25462803
microfluidic system for high-throughput immunoglobulin-e analysis from clinical serum samples.	rapid and high-throughput analytical techniques for ige that requires a small serum amount are very important, especially for pediatric patients. in these patients, blood is collected from veins, which is painful compared to fingertip blood collection. herein, a novel microfluidic system capable of high-throughput parallel analyses of allergen-specific ige from small amounts of patient serum was successfully developed. a six-plex immunoassay was constructed within a microfluidic chip, and the en ...	2015	26078132
studies of house dust mites can now fully embrace the "-omics" era.		2015	25662308
cloning, expression, purification, characterization, crystallization and x-ray crystallographic analysis of recombinant der f 21 (rder f 21) from dermatophagoides farinae.	dermatophagoides farinae is one of the major house dust mite (hdm) species that cause allergic diseases. n-terminally his-tagged recombinant der f 21 (rder f 21), a group 21 allergen, with the signal peptide truncated was successfully overexpressed in an escherichia coli expression system. the purified rder f 21 protein was initially crystallized using the sitting-drop vapour-diffusion method. well diffracting protein crystals were obtained after optimization of the crystallization conditions us ...	2015	26527267
[expression, purification and bioinformatics analysis of β-hexosaminidase of dermatophagoides farinae].	the dna fragment encoding β-hexosaminidase was synthesized, and cloned into pet-28a vector. the constructed plasmid pmd18-t-β-hexosaminidase was transformed into e. coli top 10 and followed by expression of the protein induced by iptg. sds-page result showed that the relative molecular mass of the recombinant protein was about m, 55 000. the full length of β-hexosaminidase gene was 1 410 bp. bioinformatics analysis revealed that β-hexosaminidase was composed with 469 amino acid residues with a c ...	2015	26245133
cross‑reactivity between group-5 and -21 mite allergens from dermatophagoides farinae, tyrophagus putrescentiae and blomia tropicalis.	group-5 and group-21 allergens, produced by house dust mites and storage mites are 36.6-55.8% identical in their sequences and are recognized by at least 50% of immunoglobulin (ig)e from the sera of individuals allergic to dust mites. in the present study, recombinant group-5 and ‑21 allergens from three mite species, dermatophagoides farinae (rder f 5 and 21), tyrophagus putrescentiae (rtyr p 5 and 21), and blomia tropicalis (rblo t 5 and 21), were purified from escherichia coli, and the ige re ...	2015	26238285
the dermatophagoides farinae group 22 allergen: cloning and expression in escherichia coli.	dermatophagoides farinae (hughes) (acari: pyroglyphidae) and other domestic mites produce allergens that affect people worldwide. here, the complementary dna (cdna) coding for group 22 allergen of d. farinae (der f 22) from china was cloned, sequenced, and expressed successfully.	2015	26096916
[[expression of house dust mite allergens der f1 and der f2 in nicotiana benthamiana leaves].	the class e immunoglobulins (ige) is known to recognize conformational epitopes and therefore the native conformation of recombinant allergens is essential for their using in test-systems. recombinant dermatophagoides farinae house dust mite (hdm) allergens der f1 and der f2 were expressed in bacteria escherichia coli and nicotiana benthamiana plants. it has been shown that ige in sera from children allergic to hdm recognizes der f2 expressed both in e. coli and n. benthamiana. mature form of de ...	2015	25898757
perennial aeroallergens sensitisation and risk of asthma in african children and adolescents: a case-control study.	the distribution and impact of various aeroallergens on asthma occurrence vary across regions. we investigated the association between sensitisation to perennial aeroallergens and asthma risk in children and adolescents in yaounde, cameroon.	2015	25494554
preparation of a polyclonal antibody that recognizes a unique galactoseβ1-4fucose disaccharide epitope.	galactoseβ1-4fucose (galβ1-4fuc) is a unique disaccharide unit that has been found only in the n-glycans of protostomia. we demonstrated that this unit has a role as an endogenous ligand for caenorhabditis elegans galectins. this unit is also recognized by fungal and mammalian galectins possibly as a non-self glycomarker. in order to clarify its biological function, we made a polyclonal antibody using (galβ1-4fuc)n-bsa as the antigen, which was prepared by crosslinking galβ1-4fuc-o-(ch2)2-sh and ...	2015	26025207
house dust mites possess a polymorphic, single domain putative peptidoglycan d,l endopeptidase belonging to the nlpc/p60 superfamily.	a 14 kda protein homologous to the γ-d-glutamyl-l-diamino acid endopeptidase members of the nlpc/p60 superfamily has been described in dermatophagoides pteronyssinus and dermatophagoides farinae but it is not clear whether other species produce homologues. bioinformatics revealed homologous genes in other sarcopteformes mite species (psoroptes ovis and blomia tropicalis) but not in tetranychus urticae and metaseiulus occidentalis. the degrees of identity (similarity) between the d. pteronyssinus ...	2015	26566476
characterization and analysis of a cdna coding for the group 29b (der f 29b) allergen of dermatophagoides farinae.	this study aims to acquire a recombinant allergen of der f 29b by cloning and expression, and to identify its immunogenicity. in this study, the total rna of d. farinae was extracted, cloned and expressed based on the der f 29b gene. the molecular characteristics of der f 29b was analyzed by the procedures of bioinformatics. the allergenicity of recombinant der f 29b protein was examined by western-blotting, elisa, immune inhibitory assays and skin prick test. the gene of der f 29b consisted of ...	2016	27158348
[influence of serum levels of vitamin d on ige response in schoolchildren with asthma in poor communities].	asthma is a common disease in the world and vitamin d (vit-d) has been associated with the presence and severity of this disease.	2016	27560913
heat-killed enterococcus faecalis ef-2001 ameliorates atopic dermatitis in a murine model.	recent reports have shown the immunomodulatory effect of heat-killed lactic acid bacteria. atopic dermatitis (ad) is an allergic skin disease, caused by immune dysregulation among other factors. the aim of this study was to assess the effect of heat-killed enterococcus faecalis ef-2001 (ef-2001) on ad. we established an in vivo ad model by repeated local exposure of dermatophagoides farinae extract (dfe; house dust mite extract) and 2,4-dinitrochlorobenzene (dncb) to the ears of mice. after oral ...	2016	26959058
dust mite allergen der f 4: expression, characterization, and ige binding in pediatric asthma.	house dust mite hypersensitivity affects millions of people worldwide, and although many allergens produced by house dust mite species have been identified, some of the less potent allergens remain to be studied.	2016	26842967
characterization of sarcoptes scabiei cofilin gene and assessment of recombinant cofilin protein as an antigen in indirect-elisa for diagnosis.	scabies impairs the health of humans and animals and causes heavy economic losses. traditional diagnostic methods for scabies are inefficient and ineffective, and so far there is no commercial immunodiagnostic or molecular based test for scabies.	2016	26801761
cellular gene expression induced by parasite antigens and allergens in neonates from parasite-infected mothers.	prenatal exposure to parasite antigens or allergens will influence the profile and strength of postnatal immune responses, such contact may tolerize and increase susceptibility to future infections or sensitize to environmental allergens. exposure in utero to parasite antigens will distinctly alter cellular gene expression in newborns. gene microarrays were applied to study gene expression in umbilical cord blood cell (ucbc) from parasite-exposed (para-pos) and non-exposed (para-neg) neonates. u ...	2016	27062712
effects of topical application of a recombinant staphylococcal enterotoxin a on dncb and dust mite extract-induced atopic dermatitis-like lesions in a murine model.	atopic dermatitis (ad) is a chronic inflammatory skin disease with biphasic t cell-mediated abnormalities. staphylococcal superantigens contribute to the exacerbation of inflammation in ad. the underlying immunopathological mechanisms are not fully understood.	2016	24721827