Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| an evaluation of the absorption spectra of antigens from entamoeba histolytica (ibadan strains). | by means of chromatography different fractions of antigens from entamoeba histolytica (ibadan strains) have been separated. all the different fractions have been tested for antigenic reactivity using agar gel diffusion technique. the intact antigen, as well as, the three antigenically reactive fractions were found to have ultra-violet absorption spectra in the region 258 and 278 millimicron. by virtue of this physico-chemical property amoebic extract(s) was found to simulate a mononucleotide deo ... | 1977 | 209678 |
| heat-labile enterotoxigenic escherichia coli and intestinal protozoa in asymptomatic travellers. | thirty-two asymptomatic travellers who had recently journeyed in the near, middle, and far east and had experienced a high incidence of diarrhoeal disease were screened for heat-labile enterotoxigenic escherichia coli (ent+ e. coli) and other bacterial and parasitic pathogens. six percent were colonized with ent+ e. coli and while other bacterial pathogens were not found, the intestinal protozoa giardia lamblia (13%), entamoeba histolytica (6%), entamoeba coli (6%), endolimax nana (6%), and enta ... | 1977 | 351820 |
| electron microscope studies of experimental entamoeba histolytica infection in the guinea pig. iii. histolysis of the cecum. | 1977 | 412297 | |
| radiology of invasive amebiasis of the colon. | entamoeba histolytica is a protozoan that is endemic in various parts of the world, including some areas of the united states. it may live in the large bowel in its cyst form without harming the host (commensalism) or, for as yet poorly understood reasons, invade the tissues as a trophozoite producing invasive amebiasis of the colon. in a review of over 3,000 cases of invasive amebiasis, the clinico-pathologic forms of the disease were: ulcerative rectocolitis (95%), typhloappendicitis (3%), ame ... | 1977 | 414558 |
| the prevalence of human intestinal protozoa in ibadan, nigeria. | this study shows that the two intestinal protozoa, giardia lamblia, balantidium coli in addition to entamoeba histolytica are prevalent in the ibadan population and are responsible for many of the non-bacterial diarrhoea seen in patients. a high seasonal prevalence of the intestinal protozoa in the dry months of the year was associated with the use of contaminated water. both the high infection rates recorded in adults and children under five years old, and the high frequency of association invo ... | 1977 | 563477 |
| [chemotherapeutically effective nitro compounds. 3rd communication: nitropyridines, nitroimidazopyridines and related compounds (author's transl)]. | new 2-nitropyridines and 3-nitropyridines, and 3-nitroimidazo-[1,2-a]-pyridines and related compounds (together 106) were synthesized and tested for their chemotherapeutic efficacy against trichomonads, amoebas and other organisms, such as eimeria tenella, bacteria, fungi, helminths. several 2-nitropyridines revelaed a detectable systemic effect against entamoeba histolytica (extraintestinal amoebiasis of the golden hamster) and also a weak activity against trichomonas fetus in the nmri-mouse. o ... | 1977 | 576820 |
| age and sex distribution of infections with entamoeba histolytica and giardia intestinalis in the lagos population. | stool samples were collected from 2,099 persons and examined for parasites: 9.5 percent were infected with entamoeba histolytica, 7.9 percent were infected with giardia intestinalis and 15.5 percent were infected with iodamoeba butschlii. the infections with e. histolytica and i. butschlii occurred more frequently in females than in males and adults were also more frequently infected than children. infection with g. intestinalis, on the other hand, was more prevalent in males than in females and ... | 1977 | 591169 |
| parasites of man in remote areas of central and south sulawesi, indonesia. | a biomedical survey was conducted in 10 villages in remote, high mountain valleys of central and south sulawesi, indonesia to learn whether oriental schistosomiasis was endemic and to determine the prevalences of other intestinal parasites, malaria and filariasis in those areas. although persons with oriental schistosomiasis were found in three villages of south sulawesi, follow-up inquiries revealed that these had recently migrated from a known schistosomiasis area in central sulawesi. other in ... | 1977 | 607428 |
| biomedical survey in north samar province, philippine islands. | a biomedical survey was carried out in north samar province, philipines to update information on the prevalence of parasitic and other infectious diseases. a total of 1,394 stool specimens, 1,859 blood smears and 1,274 sera were collected from persons living in 8 barrios. stools were examined for intestinal parasites, bloods smears for malaria and filariasis and sera tested for antibodies to schistosoma japonicum, entamoeba histolytica, toxoplasma gondii, influenza a and b, and japanese encephal ... | 1977 | 614707 |
| surface properties related to concanavalin a-induced agglutination. a comparative study of several entamoeba strains. | pathogenic strains of entamoeba histolytica are more easily agglutinated with concanavalin a (con a) than strains isolated from human asymptomatic carriers. all three pathogenic strains studied here were found to agglutinate with low concentrations of con a in contrast to various nonpathogenic axenic strains of amebas, characterized by their ability to grow at room temperature. our present observations suggest that the extreme susceptibility of pathogenic strains of e. histolytica to agglutinate ... | 1977 | 233919 |
| [the laboratory diagnosis of amoebic infections in man (author's transl)]. | the rapid progress made in both theoretical and practical fields in laboratory medicine, in general, and in microbiological diagnostic procedures, in particular, have also led to a significant enlargement of, and improvement in laboratory diagnosis of amoebic infections in man. in all intestinal infections with entamoeba histolytica the demonstration of the parasite is absolutely essential, but is facilitated nowadays by the great improvement in technical conditions. immunodiagnostic methods hav ... | 1977 | 842029 |
| [toxoplasmosis. anatomo clinical characteristics and morphological indentification of the parasite using the silver-impregnation technic]. | the clinical and anatomicopathological characteristics of acute and chronic toxoplasmosis are described as they affect different structures and systems. the lesion to adrenal glands is compared to that seen in disseminated herpes simplex; however, the question remains as to why in both congenital infections, necrosis of adrenal glands appears without inflammatory reaction. the investigation of special techniques for localization and identification of toxoplasma gondii "groups" or cysts, leads to ... | 1977 | 843413 |
| plasmalemmal modifications of entamoeba histolytica in vivo. | we used electron microscopy to study entamoeba histolytica trophyozoites in rectal and liver lesions. the amebal surface had bleblike structures. also noted were subplasmalemmal vacuoles and plasmalemmal extensions generally similar to the "surface lysosome" and "trigger" previously described. we found on serial sections that the plasmalemmal extensions were dendritic complexes enfolding the membrane of the subplasmalemmal vacuole and extending to contact host tissue. we postulate that membrane- ... | 1977 | 845740 |
| endemic amebiasis in an extended family. | two cousins from a large spanish-american family were simultaneously diagnosed as having amebic liver abscesses. survey of 183 extended-family members revealed that 45.7% of 162 had a positive amebiasis indirect hemagglutination test and 12.6% of 111 had cysts or trophozoites of entamoeba histolytica demonstrated in a single stool examination. a total of five family members had had liver abscesses; two deaths had occurred. in a random sample survey of the remainder of the community, only one per ... | 1977 | 889003 |
| cellular immune responses to amoebic liver abcess in the guinea-pig. | guinea-pigs infected in the liver with the biswas strain of entamoeba histolytica showed no dermal hypersensitivity but showed positive lymphocyte transformation and macrophage-migration inhibition. the time sequence showed an activated response at 4 days after infection, a full response at 8 days when the liver abscesses were resolving and a waning response at 12 days when the abscesses had healed. | 1977 | 891028 |
| [traveler's diarrhea]. | travellers' diarrhea afflicts up to 50 percent of tourists in certain geographic regions. it is a syndrome caused by a variety of pathogens, the most common of which is enterotoxigenic e. coli. other responsible pathogens include salmonellae, shigellae, entamoeba histolytica, yersinia enterocolitica, giardia lamblia and virus-like agents, seldom vibrios. symptoms persist for only a few days and are only serious in 1/5 of the cases. | 1977 | 914154 |
| amebiasis: epidemiologic studies in the united states, 1971-1974. | seven investigations of suspected foci of amebiasis between october 1971 and june 1974 lead to three conclusions. (1) a number of laboratories have vastly overdiagnosed amebiasis and have reported leukocytes in stools as entamoeba histolytica. two laboratories found to be in error were in community hospitals, and one was at a teaching hospital associated with a medical school and a school of public health. these three laboratories had been diagnosing more than 1200 cases of amebiasis a year for ... | 1978 | 619763 |
| studies on the intestinal parasites in african patients in owamboland, south west africa. | the paper gives the results of a survey of intestinal parasites among 501 in-patients drawn from four mission hospitals in owambo and kavango in northern south west africa. most of the patients (90%) were examined twice by the formol-ether concentration method, while the remaining 10% had one stool specimen examined. 10 species of helminths and 3 species of potentially-pathogenic protozoa were recorded--necator americanus, strongyloides stercoralis, strongyloides fuelleborni, hymenolepis nana, t ... | 1978 | 635971 |
| pleuropulmonary amoebiasis in ghana: a report of three cases. | three cases of pleuropulmonary amoebiasis are reported for the first time from ghana. it is suggested that where facilities for identification of the causal agent are not available a therapeutic trial should be given with drugs effective against entamoeba histolytica. | 1978 | 646939 |
| treatment of intestinal amoebiasis and giardiasis; efficacy of metronidazole and tinidazole compared. | aboriginal children who were aged from six to nine years and living in cherbourg community were treated for giardia lamblia and entamoeba histolytica infection with either the manufacturer's recommended dose of metronidazole (flagyl) or with tinidazole (fasigyn), at two-dose levels. tinidazole successfully cleared both protozoans from stools, a single dose being as effective as the longer regimens which were studied. the obvious advantage of single-dose administration is that it should permit mo ... | 1978 | 672734 |
| [redistribution of surface antigens of entamoeba histolytica and their immunochemical characteristics]. | 1978 | 697475 | |
| serologic responses in amebiasis. | serologic tests for amebiasis, utilizing antigens prepared from entamoeba histolytica hk-9, axenic culture in diamond's media, have demonstrated reproducible results with good clinical correlation. the indirect hemagglutination test was positive in 100 per cent of all cases with hepatic abscess, in 99 per cent of cases of amebic dysentery and in 60 per cent of asymptomatic carriers. a population of uninfected subjects yielded less than 1 per cent of individuals with demonstrable amebic antibodie ... | 1978 | 697482 |
| [present status of the diagnosis and treatment of invasive amebiasis caused by entamoeba histolytica. i. usefulness of ultrasonography in the diagnosis and evolution of amebic liver abscess]. | 1978 | 697485 | |
| [present status of the diagnosis and treatment of invasive amebiasis caused by entamoeba histolytica. ii. current status of hepatic scintillography]. | 1978 | 697486 | |
| [present status of the diagnosis and treatment of invasive amebiasis caused by entamoeba histolytica. iii. medical treatment of amebiasis]. | 1978 | 697487 | |
| [present status of the diagnosis and treatment of invasive amebiasis caused by entamoeba histolytica. iv. surgical treatment of the complications of invasive amebiasis]. | 1978 | 697488 | |
| serum ige levels in amoebiasis. | elevated levels of serum immunoglobulin e (ige) are found in many parasitic diseases. in order to determine the influence of entamoeba histolytica on ige levels of clinically symptomatic patients with intestinal amoebiasis, a controlled study was performed. there was no difference between the serum ige values from brazilian patients with amoebiasis and those from age-, race- and sex-matched normal brazilian controls. based also on our previous similar studies on giardiasis and chagas' disease, w ... | 1978 | 709800 |
| surface properties of entamoeba: increased rates of human erythrocyte phagocytosis in pathogenic strains. | the assertion that ingestion of human erythrocytes is restricted to invasive strains of entamoeba histolytica has not been evaluated previously by comparative studies. in this report we describe the in vitro ingestion of human erythrocytes by pathogenic and nonpathogenic entamoeba. microscopic evaluation of erythrophagocytosis by eight different entamoeba grown in culture revealed that strains of e. histolytica isolated from cases of human dysentery show a much higher rate of erythrocyte ingesti ... | 1978 | 722237 |
| cutaneous amebiasis of the eyelid with extension into the orbit. | a 4-month-old girl was admitted to hospital with extensive ulceration and erosion of the left eyelid where 17 days earlier the skin had been broken when hit by a ball. despite antibiotic therapy the lesion expanded to involve the orbit. biopsy of the eyelid on the 4th day in hospital, and examination of the eye and attached tissues removed on the 6th, failed to detect amebae until after the infant's death due to a blood transfusion 2 weeks later. re-examination of the tissues revealed abundant a ... | 1978 | 727318 |
| serological study of amoebiasis and toxoplasmosis in the malili area, south sulawesi, indonesia. | results of a serology survey in september 1972 for entamoeba histolytica and toxoplasma gondii antibodies among 915 volunteers from the malili area of south sulawesi (celebes), indonesia are presented. indirect hemagglutination antibody titers for amoebiasis were found in 22.7% of the sampled population while 9.5% demonstrated antibody titers greater than or equal to 1:128. the frequency distribution of antibody titers were similar by sex and age. there was no significant correlation between sto ... | 1978 | 751213 |
| [medical practice in indians of the colombian amazon. epidemiology of intestinal parasitoses]. | in spite of the presence of a physician, the real pathology of an indian population of colombie is not very well known. some of ill people are probably entrusted to the traditional "chaman". the western medicine is symptomatic, curative and not preventive. intestinal nematodiasis and amoebiasis are the major problem. prevalences are: ascaris 46%, trichuris 30%, ankylostoma 39%, strongyloides 40%, entamoeba histolytica 30%. among this slowly evoluting population parasitism is related with cultura ... | 1978 | 754884 |
| serology of amebiasis using the fiax-tm system. | a new fluorescent immunoassay system (fiax tm) has permitted development of a promising serologic test for amebiasis. the test is similar but far superior to the soluble antigen fluorescent antibody test. the main differences are the unique stiq tm sampler and the flourometer itself. quantitative results can be obtained from a single standard dilution of serum. the procedure is simple to perform, gives objective readings, and uses the proven principle of immunofluorescence. it is possible to tes ... | 1978 | 358934 |
| [intestinal amebiasis in children. i. etiological study of colonic lesions of the rectosigmoid colon]. | a total of 253 children from two months to 12 years old, who had diarrheic or dysenteric syndromes, were studied from the rectosigmoidoscopic and parasitologic points of view. in addition, 112 and 20 of these patients were also studied bacteriologically and virologically, respectively. only in 28 patients torphozoites of entamoeba histolytica were found by means of direct microscopic examination, staining techniques and amoebal cultures. enteropathogenic bacteria were isolated in 41 of the 112 c ... | 1978 | 358935 |
| human parasitoses of the malili area, south sulawesi (celebes) province, indonesia. | a biomedical survey was conducted in 9 villages in the malili area of south sulawesi, indonesia. blood specimens were examined for malaria and microfilariae; stool specimens were examined for intestinal parasites. malaria parasitemias were rare; plasmodium falciparum was detected in 10 and p. vivax in 11 of 985 blood smears. malayan filariasis was endemic to all villages surveyed. the overall prevalence of detectable microfilaremias was 15%, varying from 34% in kawata to 1% in nuha. microfilaria ... | 1978 | 364674 |
| new and old agents in diarrhea: a prospective study of an indigenous adult african population. | we conducted a prospective study 77 indigenous african adults with acute diarrhea seeking care at the major hospital in nairobi, kenya, to determine the major pathogens responsible for this syndrome in adults. fecal and blood specimens were collected and examined for enteric bacterial pathogens, viruses, and parasites. in 13 (26%) inpatients and 11 (49%) outpatients shigella was found, and heat-labile and heat-stable forms of enterotoxigenic escherichia coli were found in 9 (18%) inpatients and ... | 1978 | 209697 |
| cryopreservation of parasitic protozoa. | 1978 | 233753 | |
| chemotherapeutically active nitro compounds. 4.5-nitroimidazoles (part ii). | more than 170 1-methyl-5-nitroimidazoles substituted in the 2-position via an aminomethyl, thiomethyl, sulphinylmethyl or sulphonylmethyl group were synthesized and tested for their effect against various protozoa. in the nmri mouse which had been i.p. infected with trichomonas fetus 2 compounds showed an effect superior to that of tinidazole and 31 showed similarly good efficacy as that compound. in comparison with metronidazole 54 preparations proved to be distinctly more active, while 34 othe ... | 1978 | 350230 |
| aspects of the pharmacology and pharmacokinetics of nitroimidazoles with special reference to tinidazole. | tinidazole has been reported to be highly effective against trichomoniasis, giardiasis and amoebiasis. in vitro, tinidazole is more active against trichomonads than metronidazole and possesses antiprotozoal activity at least comparable to metronidazole against entamoeba histolytica, tinidazole gives higher serum levels in animals following oral administration than metronidazole and is well distributed in organs and tissues. when tinidazole or metronidazole is given to healthy volunteers at a dos ... | 1978 | 350561 |
| treatment of symptomatic intestinal amoebiasis with tinidazole. | tinidazole was given in a single dose of 2g a day for 2 days to 17 adults, and in a single dose of 50mg/kg bodyweight daily for 3 days to 4 children, with symptomatic, but not severe, intestinal amoebiasis. all 21 patients showed clinical improvement but only 20 (95%) were parasitologically cured as evinced by failure to demonstrate entamoeba histolytica in 3 stool samples collected on the 4th, 15th and 22nd day after treatment. two subjects had mild nausea within a few hours of taking the first ... | 1978 | 350562 |
| [coproculture for "entamoeba histolytica". study of 170 cases examined at the clinic for tropical and infectious diseases at the universita di roma in the 1st semester of 1978]. | 1978 | 226012 | |
| [current status of the chemotherapy of amebiasis]. | 1978 | 226448 | |
| the interaction between entamoeba histolytica and syphacia obvelata infection in mice. | mice naturally infected with syphacia obvelata were challenged intracaecally with entamoeba histolytica trophozoites. they showed a higher level of amoebic infection than mice without worms. mice with the heaviest syphacia infection were the most susceptible to amoebic infection. it appears that a concomitant infection with helminthic parasites residing in the caecum alters the outcome of an amoebic infection. | 1978 | 216325 |
| [azotized emetine analogues: synthesis and evaluation of the anti-amebic and anti-tumoral action of aza-3 emetine and attempted synthesis of aza-2 emetine]. | the synthesis of the n,n-bis-(acetylhomoveratrylamido)-1-hydroxymethyl and 1-carboxypropylamines (iii a) and (iii b) is described. until now, these molecules could not been cyclized to the corresponding isoquinoline compounds. an attempted synthesis of 3-azaemetine by condensing homoveratrylamine with acetonedicarboxylic acid or its esters did not yield the expected diamide (xiv). however, by subjecting 1,3-bis-(1,2,3,4-tetrahydro-6,7-dimethoxy-1-isoquinolyl)acetone (xv) to a reducing aminoalkyl ... | 1978 | 216576 |
| clonal growth of entamoeba histolytica and other species of entamoeba in agar. | this is the first description of a method for growing axenized entamoeba histolytica as colonies from single cells in agar suspension. factors affecting the efficiency of colony formation included unknown characteristics of the amebal strain, age of cells used, and the type and concentration of agar employed. colony formation was dependent upon filling deep vessels (culture tubes or tissue culture flasks) with agar, probably to maintain reduced oxygen tension, and upon solidifying the agar rapid ... | 1978 | 216801 |
| [indications for the use of antibiotics for protozoan infections]. | 1978 | 216956 | |
| high rates of enteric protozoal infections in selected homosexual men attending a venereal disease clinic. | stool specimens from 89 high-risk, sexually active homosexual men were screened for enteric protozoal cysts. patients whose specimens contained nonpathogenic cysts were investigated further by examination of fresh purged specimens. a total of 27 of the initial specimens (30%) contained protozoal cysts. twenty-three patients (26%) harbored pathogens. there were 18 cases (20%) of amebiasis and 11 cases (12%) of giardiasis. six men (7%) had concurrent amebiasis and giardiasis. the presence of infec ... | 1978 | 217110 |
| restoration of virulence of axenically cultivated entamoeba histolytica by cholesterol. | the lost pathogenicity of two strains of entamoeba histolytica, one isolated in 1924 and the other in 1967, grown in axenic culture for the past 5 and 6 years respectively, was restored by supplementing the culture medium with cholesterol through a number of transfers. the number of passages in the cholesterol-supplemented medium, necessary to restore a certain degree of pathogenicity of the two strains in hamsters, was proportional to the total time of in vitro cultivation of the strain, and no ... | 1978 | 224992 |
| a clinicopathologic study on patients suffering from "chronic dysentery". | fifty adult patients with the self diagnosis of "chronic dysentery" were studied. their faeces were examined microscopically; they were sigmoidoscoped and their rectal mucosa were examined histologically. most of them had irritable bowel syndrome. their sigmoidoscopic appearances were normal and the histology showed only mild increase in the numbers of round cells in the lamina propria. this was regarded as normal for the local population. ten patients showed cysts of entamoeba histolytica in th ... | 1978 | 230812 |
| entamoeba histolytica: uptake of purine bases and nucleosides during axenic growth. | 1978 | 210037 | |
| modified stuart medium--a transport and maintenance medium for entamoeba histolytica. | 1978 | 210120 | |
| pathogenicity of entamoeba histolytica--effect of cholesterol on the virulence of strains of amoebae. | 1978 | 210121 | |
| vaccination against hepatic amebiasis in hamsters. | 1978 | 210268 | |
| indirect fluorescent antibody test in amoebiasis using axenic entamoeba histolytica. | 1978 | 211081 | |
| growth pattern of axenic entamoeba histolytica in different sera and the antiamoebic activity of specific antisera. | 1978 | 211082 | |
| experimental muscular amebiasis in hamsters as a biological model. | trophozoites of entamoeba histolytica maintained in vitro in pavlova's medium were inoculated by deep intramuscular injection into the proximal left hindleg of hamsters. thioglycollate medium was utilized as a successful vehicle to induce the infection. the invasion of the muscular tissue by the vegetative forms caused the formation of abscesses with great destruction of muscular fibers. the lesions were limited to the muscular tissue of the femoral area. the number of trophozoites, the medium o ... | 1978 | 211747 |
| [ultramicroscopic structure of the cyst wall of entamoeba invadens, e. histolytica and e. coli]. | the ultrastructure of the wall of e. invadens cysts induced in axenic cultures was compared by transmission electron microscopy of thin sections, with that of e. histolytica and e. coli cysts obtained from feces of human asymptomatic carriers. the cyst wall of the three species studied had similar ultrastructural features; in all, the wall was composed mainly by the aggregation of filaments adjacent to the plasma membrane. the cytoplasmic components which might be involved in the synthesis and d ... | 1978 | 211940 |
| [instability of the dna content in entamoeba histolytica]. | dna content was repeatedly determined during a 10-month period on axenically cultured tropho-zoites from three strains (hk-9:nih, hm-2:imss and hm-3:imss) and a clone of hk-9:nih strain of entamoeba histolytica, by means of the diphenylamine reaction. average dna content was more or less variable in all of them. according to their extent, variations were: 1) large (almost four times in strain hm-2:imss), or 2) small (1.5 times in the clonal isolate). with this relatively small sample, three stat ... | 1978 | 211941 |
| isolation and sedimentation analysis of rna from entamoeba histolytica. | 1978 | 211942 | |
| [amino acid composition in total proteins of strains of entamoeba histolytica in axenic cultures]. | in this paper we detail the amino acid composition of total protein hydrolyzates from trophozoites of two entamoeba histolytica strains (hk-9:nih and hm-2:imss) cultured axenically in tp-s-1 medium. since we obtained nearly identical results in both strains, amino acid patterns may constitute an additional taxonomic criterion of the species. our data are strikingly different from those found previously by other workers with total proteins from hk-9:nih trophozoites cultured in a bacteria-conditi ... | 1978 | 211943 |
| uptake of selected pyrimidines by axenically grown entamoeba histolytica. | this study was conducted to determine the mode(s) of uptake of selected pyrimidine bases and nucleosides by axenically grown entamoeba histolytica. two-minute uptake studies with 3h-labeled compounds showed the following: 1) uridine and cytidine are taken up, in part, by a carrier mediated system; 2) uracil, cytosine, thymine and thymidine enter amebae via passive diffusion; both cytidine and uridine are taken up by passive diffusion when present at exogenous concentration greater than 500 mum; ... | 1978 | 211944 |
| [immunochemistry of immunogenic proteins of entamoeba histolytica]. | entamoeba histolytica (hk-9: imss) trophozoites were separated from the culture medium and washed and submitted to the effects of ultrasound during 10 minutes. after 10,000 rpm x 30 centrifugation, the supernatant fluid was separated in four fractions by sephadex g-200 gel chromatography. these fractions were lyophilized, redisolved in a lesser volume and dyalized against isotonic saline solution. antigen-antibody precipitant reactions between each of them and two rabbit antiserums were demonstr ... | 1978 | 211945 |
| [cytopathogenic activity of extracts e. histolytica trophozoites]. | previous studies have not shown any correlation between enzyme activities and pathogenicity of entamoeba histolytica. the activities analyzed were selected without having proved their involvement in the cytopathogenicity. we are using the reverse approach to study the cytopathogenic factors, i.e., to detect and characterize first the cytopathic effect of amebic extracts on animal cells, and subsequently to try to identify the components involved and to explore their correlation with amebic virul ... | 1978 | 211946 |
| [effects of nordihydroguairetic acid and ethanol on the growth of entamoeba invadens]. | the effects of the alcoholic extract (resin) obtained from larrea tridentata, of nordihydroguiaretic acid (ndga) and of ethanol over axenic cultures of entamoeba invadens pz strain were studied. the activity of these substances was demonstrated in growht curves. inocula of 1-3 x 10(5) trophozites by milliliter in culture tubes containing 11 ml of tp-s-1 medium were done, and incubated at 25 degree c. in each experiment the follwoing was done: a) tipical growth curve as a control, b) curve with t ... | 1978 | 211947 |
| amebal viruses and the virulence of entamoeba histolytica. | 1978 | 211948 | |
| variable toxicity within the hk 9 strain of entamoeba histolytica. | 1978 | 211949 | |
| surface ultrastructure of entamoeba histolytica; a study by high voltage transmission electron microscopy (hvtem) and scanning electron microscopy (sem). | 1978 | 211950 | |
| the development and standaridization of an elisa method for the detection of entamoeba histolytica antigens in fecal samples. | recognizing the difficluties associated with the microscopic detection and identification of entamoeba histolytica in fecal samples, an immunochemical method of detection of e. histolytica antigens in fe-es has been developed. for a number of reasons, e.g., the known fragility of e. histolytica trophozoites, it was felt that intestinal amebiasis would be associated with detectable levels of e. histolytica anti-ens in feces from infected patients. a technique for harvesting amebic antigens in fec ... | 1978 | 211951 |
| [identification of entamoeba histolytica membrane antigens with antibodies of amebiasis patients]. | the specificity of sera from patients with amoebiasis was assayed on trophozoites of e. histolytica hk9, hm15 and hm2 cultured axenically and monoxenically. glutaraldehyde fixed cell were incubated with samples of serum, the excess of igs was washed out and the presence of unbound material of specifically bound antibodies was measured with radiolabeled protein a from staphylococcus aureus. a positive reaction with hk9 was observed in 18 per cent of the 66 sera tested. an immune crossreaction res ... | 1978 | 211952 |
| [phagocytosis of human erythrocytes by entamoeba histolytica. quantitative study]. | the degree of eythrophagocytosis of two recently isolated strains of e. histolytica was measured by microscopic examination. amebas isolated from a patient with amebic rectocolitis (strain hm22:imss, monoxenic) ingested human red blood cells faster and in larger numbers than trophozoites isolated from an asymptomatic carrier (hm27:imss, monoxenic). the results suggest that an increased rate of phagocytosis could be one of the surface properties characteristic of the invasive strains of e. histol ... | 1978 | 211953 |
| production of cecal lesions in newborn guinea pigs with axenically cultivated entamoeba histolytica. | the production of cecal lesions with axenically cultivated entamoeba histolytica in an experimental animal is reported for the first time. forty-three newborn guinea pigs (one to five days old) were inoculated via laparatomy with 2.5 x 10(5) to 2 x 10(6) strain hm-1-imss amebae. of 23 animals surviving to the 7th postinoculation day all showed cecal lesions at necropsy regardless of the age of the animal when used or the number of amebae received. ten animals had lesions visible to the unaided e ... | 1978 | 211954 |
| [culture and establishment of new mexican strains of entamoeba histolytica]. | mixed and monoxenic cultures of rectal exudates from three patients with amebiasis of colon, and of feces from three asymptomatic carriers of entamoeba histolytica obtained after administration of a saline cathartic, and from a case of exudate of the perianal cutaneous lesion border produced by this parasite, were done. the culture media used were for: mixed culture, boeck and drbohlav modified culture and, for monoxenic culture, diamond's monophasic tp-s-1 medium without vitamin mixture and usi ... | 1978 | 211955 |
| cytotoxicity of a cell free extract of entamoeba histolytica. | previous attempts to demonstrate toxicity of cell-free extracts prepared from axenically cultivated e. histolytica have proved unsuccessful. we report here the successful production of cytopathic effect by cell-free extracts of two strains (hm-1 and hk-9) of e. histolytica in tissue culture monolayers. the cytotoxic effect demonstrated was sensitive to serum in the assay medium. failure of past investigations to demonstrate cytotoxic activity may be explained by the presence of serum in the assa ... | 1978 | 211956 |
| clonal growth of entamoeba in agar: some applications of this technique to the study of their cell biology. | a new technique for growing single entamoeba trophozoites into colonies in agar has been developed. this method depended upon axenic cultivation and utilized diamond's new tyi-s-33 medium. the present paper describes several different types of experiment which have utilized the agar technique. a) cloning. the isolation of clones from agar way easy and successful. clones of the hm-1 strain did not differ in virulence for newborn hamster liver or in colony morphology or colony forming efficiency. ... | 1978 | 211957 |
| restoration of virulence of axenically-cultivated entamoeba histolytica by cholesterol. | the lost pathogenicity of two strains of entamoeba histolytica, one isolated in 1924 and the other in 1967, grown in axenic culture for the past five and six years respectively, was restored by supplementing the culture medium with cholesterol through a number of transfers. the number of passages in the cholesterol supplemented medium, necessary to restore a certain degree of pathogenicity of the two strains in hamsters, was proportional to the total time of in vitro cultivation of the strain, a ... | 1978 | 211958 |
| [an in vitro model for the quantitative study of e. histolytica virulence]. | an experimental model for the quantitative study of the cytopathic effect of e. histolytica is described. confluent cultures of the mdck cell line, which form a monolayer with morphological and electrophysiological features of an epithelium were used. trophozoites of the strains hm1:imss, hm2:imss and hm3:imss, axenic produced variable degrees of cytopathic effects of mdck cultures each one characteristic for a given strain. the degree of damage induced by the different strains of amebas was mea ... | 1978 | 211959 |
| [interaction between entamoeba histolytica and the cecal epithelium in the guinea pig]. | the early interaction between e. histolytica trophozoites and the intestinal mucosa was studied by light and electron microscopy using sheets of isolated epithelium of the guinea pig colonic mucosa. the integrity of the isolated epithelium was confirmed by scanning electron microscopy. after 30 minutes of interaction, the amebas adhered first, and penetrated subsecuently the epithelium of the colon at the interglandular regions. the results suggest that the trophozoites of e. histolytica invade ... | 1978 | 211960 |
| [ultramicroscopic study of the invasion of the mucosa of the colon by entamoeba histolytica in axenic cultures]. | animal models have been utilized to study experimentally produced intestinal amebiasis. the guinea pig has been the animal of choice to study the mechanisms by which trophozoites of e. histolytica produce colonic lesions. the purpose of this study was to produce lesions in the colon of newborn guinea pigs by the injection of e. histolytica, hm-1 imss, strain grown axenically, and to characterize the early changes with the light and electron microscopes. a total of 35 guinea pigs, one to two days ... | 1978 | 211961 |
| [serology with polysaccharides of entamoeba histolytica]. | the immunochemical activity of a polysaccharidic antigen derivated from e. histolytica was studied. this polysaccharide was tested by means of counterimmunoelectrophoresis (cie) and radial immunodiffusion (idr) against sera from patients with diagnosis of intestinal and hepatic amebiasis. in order to perform a test most significative to the problem sera, these were submitted previously to passive hemagglutination, cie and idr with total ameba antigen. in hepatic amebiasis it can be observed that ... | 1978 | 211962 |
| [activity of the alternative complement pathway by various axenic strains of entamoeba histolytica]. | the activation of the alternative pathway of complement by axenic cultures of e. histolytica is reported. the activation is carried out in different proportions by several axenic strains, i.e., hk9:nih, hm1:imss, hm2:imss and hm3:imss; the end result consists of a destruction of the parasite as well as consumption of the serum complement. the participation of the alternative pathway was studied in the presence of egta and mg++, zymosan, heat-inactivation at 50 degree c for 20 min and immunoelect ... | 1978 | 211963 |
| [immunite reaction of hamsters infected or immunized with entamoeba histolytica]. | the immune response in hamsters either infected or immunized with entamoeba histolytica was studied. the response was tested by determination of antibodies by the counterimmunoelectrophoresis technique and by lymphocyte transformation induced by the amebic antigen. the results obtained showed differences among both groups when the ability of amebic antigen to induce 3h-thymidine incorporation of lymphocyte cultures is compared. however, the response of lymphocyte cultures to concanavalin a was s ... | 1978 | 211964 |
| [induction of protective anti-ameba immunity with new antigens in the suckling hamster. a. introduction]. | 1978 | 211965 | |
| [induction of protective anti-ameba immunity with new antigens in the suckling hamster. b. antigenic material]. | 1978 | 211966 | |
| entamoeba histolytica: iron and nutritional immunity. | 1978 | 211967 | |
| [determination of an anti-ameba antigen in the feces by the elisa method (enzyme-linked immunosorbent assay) for the identification of entamoeba histolytica]. | 1978 | 211968 | |
| [a new hemagglutination method to determine antibodies against entamoeba histolytica]. | a passive hemagglutination method useful for the determination of antibodies against entamoeba histolytica is described. the e. histolytica antigen is attached to group "o" human red blood cells by means of crcl3. the specificity and sensitivity of the crcl3 method was compared with other hemagglutination methods, i.e., tannic acid method and a commercial preparation, as well as counterimmunoelectrophoresis. the crcl3 method showed a reactivity similar to the tannic acid hemagglutination techniq ... | 1978 | 211969 |
| achievements and obstacles in research for new anti-amoebic drugs. | 1978 | 211970 | |
| inorganic pyrophosphatase and nucleoside diphosphatase in the parasitic protozoon, entamoeba histolytica. | 1978 | 212030 | |
| biomedical survey in the moluccas, indonesia. | 1978 | 212835 | |
| serial measurement of total and parasite-specific ige in an african population infected with entamoeba histolytica. | 1978 | 212850 | |
| a new medium for the axenic cultivation of entamoeba histolytica and other entamoeba. | 1978 | 212851 | |
| [invasion of the mucosa of the colon by entamoeba histolytica. recent observations]. | 1978 | 213335 | |
| [technics of measuring the motive force of very small ameboid cells]. | a technic have been perfected which measures directly the motive force of very small ameboid cells and indicates the tropism of the amoeba. by using this technic, the motive force of entamoeba histolytica was measured. it varied between +/- 1.5 x 10-4 dyne. we have also shown chimiotactism in entamoeba histolytica. the metabolic coumpound excreted by this cell induced negative tropism. | 1978 | 213820 |
| experimental amebiasis. iii. a rapid in vitro assay for virulence of entamoeba histolytica. | a rapid and simple assay for cytopathogenicity of axenically cultivated entamoeba histolytica has been developed employing baby hamster kidney (bhk) or mouse 3t3 cells conventionally tissue cultured. three of the twelve amebal strains tested produced total destruction of the bhk cell monolayer in 2--3 hours, and these three strains are the three most virulent strains for the newborn hamster liver. two additional strains were of moderate cytopathogenicity in vitro and of moderate virulence in viv ... | 1978 | 213981 |
| hepatic amebiasis in spider monkeys. | in the past, investigators have been able to produce hepatic amebiasis in laboratory animals only by direct introduction of parasites into the liver or its vasculature, or by other artificial manipulations. a natural model of human visceral amebiasis has been lacking. we document an extensive outbreak of amebic dystentery which took place in a colony of spider monkeys; severe hepatic abscesses occurred in many animals. the spider monkey is highly susceptible to infection with entamoeba histolyti ... | 1978 | 213982 |
| the presence of liver auto-antibodies induced by entamoeba histolytica in the sera from both naturally infected humans and immunized rabbits. | auto-antibodies against normal human liver have been detected in the sera of humans with highly positive indirect hemagglutination (iha) amebiasis titers and with clinically-proven amebic liver abscess. sera of amebiasis patients and rabbits immunized with killed entamoeba histolytica were tested for anti-amebic antibodies by the iha test and for auto-antibodies by the complement fixation test, using the antigens prepared from extracts of human liver and rabbit liver. a direct correlation was fo ... | 1978 | 213983 |
| immunologic studies on hamsters infected with entamoeba histolytica. | the serologic and cell-mediated immune responses of hamsters exposed to 2 strains of entamoeba histolytica (hm-1 and hm-19) were evaluated by a series of in vitro tests. the pathogenicity of the 2 strains was evaluated in terms of their ability to produce liver abscesses and spleen enlargement. antibody response was evaluated by the indirect hemagglutination test. the cellular immune response was assayed by increased dna synthesis by lymphocytes and migration inhibition of macrophages. | 1978 | 214539 |
| susceptibility of various strains of mice to entamoeba histolytica. | 1978 | 214540 | |
| [imported protozoan and worm infections]. | 1978 | 214675 | |
| [effect of bacteria on the virulence of an entamoeba histolytica strain long growing in the presence of metronidazole]. | 1978 | 214693 | |
| the differentiation of invasive and non-invasive entamoeba histolytica by isoenzyme electrophoresis. | cultures of 85 stocks of entamoeba histolytica were compared by electrophoretic patterns of three enzymes: glucosephosphate isomerase, phosphoglucomutase and l-malate: nadp+ oxidoreductase (oxaloacetate-decarboxylating). four groups of e. histolytica stocks have been distinguished. 18 of these stocks were derived from individual patients with clinical amoebiasis and all of these were of one enzyme group, which did not occur among the other stocks. | 1978 | 214906 |