Publications

proteomic methods applied to the analysis of immobilized biocatalysts.	methods adapted from proteomics can directly characterize proteins present in immobilized biocatalysts. complete hydrolysis followed by hplc analysis of tyr and phe estimates total protein bound, and is preferable to conventional difference methods, as tested with subtilisin carlsberg on silica. this new method shows that various treatments give quantitative desorption of proteins immobilized by adsorption. intact desorbed proteins may be analyzed by electrospray mass spectrometry. the candida a ...	2006	16807923
an efficient two step purification and molecular characterization of beta-galactosidases from aspergillus oryzae.	beta-galactosidases (beta-d-galactoside-galactohydrolases (ec 3.2.1.23), lactases) are important industrial enzymes used for the hydrolysis of lactose from milk and milk whey. these enzymes are produced by different organisms and purified by multi-step procedures. the multi-step purification schemes are cost and time ineffective which can also lead to poor yield, denaturation and loss of enzymatic activity. in our study, extracellular beta-galactosidase from mutant strain aspergillus oryzaeh26-1 ...	2006	16865665
expression of aspergillus oryzae phytase gene in aspergillus oryzae rib40 niad(-).	aspergillus oryzae rib40 niad(-) was transformed using a plasmid constructed with the a. oryzae phytase gene and pnan8142 vector. the culture broth of the transformant, which was grown in a medium containing starch as a carbon source and polyvinylpyrrolidone showed phytase activity of a maximum of 2.0 units ml(-1) at 37 degrees c, ph 5.5.	2006	17270723
optimization of multienzyme production by two mixed strains in solid-state fermentation.	f(3) and f(4) strains of aspergillus niger were screened from five strains of fungi to produce multienzyme preparations (containing cellulase, hemicellulase, glucoamylase, pectinase, and acidic proteinase) as dietary supplementation. enzyme activities indicated that 1:4 (f(3) to f(4)) was the optimum mixture proportion, and 0.3% (w/w) was the preferable pitching rate. in bran mash containing 54.5% (w/w) water, f(3) and f(4) could produce the supplementation better when cultured 30 to 36 h at 30 ...	2006	16802152
high-throughput genotyping of filamentous fungus aspergillus oryzae based on colony direct polymerase chain reaction.	high-throughput genotyping of aspergillus oryzae was achieved using an fta card for the extraction of a genomic dna template for polymerase chain reaction from a fungal colony growing on an agar plate. this method was then applied to detect other fungal species from agar slants and food materials. this method offers a convenient tool for the genotyping of filamentous fungi without using an organic solvent or specialized equipment.	2006	17270725
discovery, cloning and heterologous expression of secreted potato proteins reveal erroneous pre-mrna splicing in aspergillus oryzae.	a novel transposon assisted signal trapping (tast) technology, developed to specifically select only the secreted proteins, was used to discover novel extracellular plant proteins from solarium tuberosum infected with phytophthora infestans. analysis of 384 hits provided 191 p. infestans and s. tuberosum sequences of secreted proteins, with an approx. 2/3 of these originating from potato. subsequent screening for interesting genes was carried out using bioinformatics. a selected variety of the d ...	2006	16814894
thermostability of native and pegylated myceliophthora thermophila laccase in aqueous and mixed solvents.	a commercial preparation of laccase (ec 1.10.3.2), cloned from myceliophthora thermophila and expressed in aspergillus oryzae (mtl), was purified and modified by conjugation with poly(ethylene glycol) (m(r) = 5000) and is labeled peg-mtl. native enzyme was found to have a molecular mass of 80 kda, as determined by gel filtration, and 110 kda, by sds-page. the oxidative dimerization of 2,6-dimethoxyphenol (dmp) to produce the corresponding dibenzoquinone was catalyzed by mtl in a manner comparabl ...	2006	16848420
aovps24, a homologue of vps24, is required for vacuolar formation which could maintain proper growth and development in the filamentous fungus aspergillus oryzae.	vps24 (vacuolar protein sorting) is a component of escrt iii (endosomal sorting complex required for transport), which is required for the formation of mvb (multivesicular body). we have isolated the vps24 homologue gene, aovps24, from the filamentous fungus aspergillus oryzae, and analyzed the localization of aovps24 using egfp. aovps24 was localized in the cytoplasm and late endosome-like structures. furthermore, we constructed an aovps24 disruptant, which showed impaired growth, conidiation, ...	2006	16857172
functional analysis of the atg8 homologue aoatg8 and role of autophagy in differentiation and germination in aspergillus oryzae.	autophagy is a well-known degradation system, induced by nutrient starvation, in which cytoplasmic components and organelles are digested via vacuoles/lysosomes. recently, it was reported that autophagy is involved in the turnover of cellular components, development, differentiation, immune responses, protection against pathogens, and cell death. in this study, we isolated the atg8 gene homologue aoatg8 from the filamentous fungus aspergillus oryzae and visualized autophagy by the expression of ...	2006	16896216
differential distribution of the endoplasmic reticulum network as visualized by the bipa-egfp fusion protein in hyphal compartments across the septum of the filamentous fungus, aspergillus oryzae.	we visualized the endoplasmic reticulum (er) network by expression of the bipa-egfp fusion protein in the filamentous fungus, aspergillus oryzae, and focused on the spatial difference of the er distribution throughout hyphae. the er formed an interconnected network with motility and displayed a gradient distribution from the apical region. the er was also found as a tubular network along the septum, which was formed soon after the completion of septation. discontinuity of the er network distribu ...	2006	16759887
food-processing enzymes from recombinant microorganisms--a review.	enzymes are commonly used in food processing and in the production of food ingredients. enzymes traditionally isolated from culturable microorganisms, plants, and mammalian tissues are often not well-adapted to the conditions used in modern food production methods. the use of recombinant dna technology has made it possible to manufacture novel enzymes suitable for specific food-processing conditions. such enzymes may be discovered by screening microorganisms sampled from diverse environments or ...	2006	16769167
reduction of flatus-inducing factors in soymilk by immobilized alpha-galactosidase.	alpha-galactosidase from aspergillus oryzae was immobilized on chitosan beads using glutaraldehyde as a cross-linking agent. the general properties of free and immobilized enzymes were determined. the optimum ph for the free and immobilized enzymes was 4.8 and 4.6 respectively. the optimum temperature for the free enzyme was 50 degrees c, whereas that of immobilized enzyme was increased to 56 degrees c. kinetic parameters were determined with synthetic substrate (p-nitrophenyl alpha-d-galactopyr ...	2006	16780418
chemical components, palatability, antioxidant activity and antimutagenicity of oncom miso using a mixture of fermented soybeans and okara with neurospora intermedia.	the enzyme activities of aspergillus oryzae on koji (malted rice) and neurospora intermedia on s-oncom and o-oncom (fermented soybeans and okara with n. intermedia, respectively) were compared. the major enzymes of n. intermedia were different from those of a. oryzae, and the enzyme activities of o-oncom were extremely higher than those of s-oncom. s5-miso, s10-miso and s9o1-miso replacing 50% or 100% of steamed soybeans with s-oncom or a 9 : 1 mixture of s-oncom and o-oncom, respectively, were ...	2006	16967767
development of aflp-derived, functionally specific markers for environmental persistence studies of fungal strains.	the ability to rapidly identify and quantify a microbial strain in a complex environmental sample has widespread applications in ecology, epidemiology, and industry. in this study, we describe a rapid method to obtain functionally specific genetic markers that can be used in conjunction with standard or real-time polymerase chain reaction (pcr) to determine the abundance of target fungal strains in selected environmental samples. the method involves sequencing of randomly cloned aflp (amplified ...	2006	16699570
lentinula edodes tlg1 encodes a thaumatin-like protein that is involved in lentinan degradation and fruiting body senescence.	lentinan is an antitumor product that is purified from fresh lentinula edodes fruiting bodies. it is a cell wall component, comprising beta-1,3-glucan with beta-1,6-linked branches, which becomes degraded during postharvest preservation as a result of increased glucanase activity. in this study, we used n-terminal amino acid sequence to isolate tlg1, a gene encoding a thaumatin-like (tl) protein in l. edodes. the cdna clone was approximately 1.0 kb whereas the genomic sequence was 2.1 kb, and co ...	2006	16648221
evaluation of certain food additives.	this report represents the conclusions of a joint fao/who expert committee convened to evaluate the safety of various food additives, with a view to recommending acceptable daily intakes (adis) and to prepare specifications for the identity and purity of food additives. the first part of the report contains a general discussion of the principles governing the toxicological evaluation of food additives (including flavouring agents), assessments of intake, and the establishment and revision of spe ...	2006	17069402
in vivo expression of udp-n-acetylglucosamine: alpha-3-d-mannoside beta-1,2-n-acetylglucosaminyltransferase i (gnt-1) in aspergillus oryzae and effects on the sugar chain of alpha-amylase.	udp-n-acetylglucosamine: alpha-3-d-mannoside beta-1,2-n-acetylglucosaminyltransferase i (gnt-i) is an essential enzyme in the conversion of high mannose type oligosaccharide to the hybrid or complex type. the full length of the rat gnt-i gene was expressed in the filamentous fungus aspergillus oryzae. a microsomal preparation from a recombinant fungus (strain ng) showed gnt-i activity that transferred n-acetylglucosamine residue to acceptor heptaose, man(5)glcnac(2). the n-linked sugar chain of ...	2006	17090929
chemoprevention of mouse urinary bladder carcinogenesis by fermented brown rice and rice bran.	fermented brown rice by aspergillus oryzae (fbra) has been shown to be a potent anti-carcinogenic compound. here, we investigated the modifying effects of dietary feeding with a naturally occurring anti-oxidant fbra on n-butyl-n-(4-hydroxybutyl)-nitrosamine (oh-bbn)-induced urinary bladder carcinogenesis in male icr mice. five-week-old male icr mice were divided into 7 groups, and groups 1-5 were given oh-bbn (500 ppm) in drinking water for 6 weeks starting at 7 weeks of age. groups 2 and 3 were ...	2006	16465408
succession of bacterial and fungal communities during a traditional pot fermentation of rice vinegar assessed by pcr-mediated denaturing gradient gel electrophoresis.	denaturing gradient gel electrophoresis (dgge) based on small subunit rrna gene was applied to a traditional rice vinegar fermentation process in which the conversion of rice starch into acetic acid proceeded in a pot. the fungal dgge profile indicated that the transition from aspergillus oryzae to saccharomyces sp. took place at the initial stage at which alcohol production was observed. the early stage was characterized by the coexistence of saccharomyces sp. and lactic acid bacteria. almost a ...	2006	16499984
substrate specificity of aspergillus oryzae family 3 beta-glucosidase.	among glycoside hydrolases, beta-glucosidase plays a unique role in many physiological and biocatalytical processes that involve the beta-linked o-glycosyl bond of various oligomeric saccharides or glycosides. structurally, the enzyme can be grouped into glycoside hydrolase family 1 and 3. although the basic ("retaining, double-displacement") mechanism for the catalysis of family 3 beta-glucosidase has been established, in-depth understanding of its structure-function relationship, particularly ...	2006	16650812
proteomic analysis of extracellular proteins from aspergillus oryzae grown under submerged and solid-state culture conditions.	filamentous fungi are widely used for the production of homologous and heterologous proteins. recently, there has been increasing interest in aspergillus oryzae because of its ability to produce heterologous proteins in solid-state culture. to provide an overview of protein secretion by a. oryzae in solid-state culture, we carried out a comparative proteome analysis of extracellular proteins in solid-state and submerged (liquid) cultures. extracellular proteins prepared from both cultures sequen ...	2006	16672490
production of high-content galacto-oligosaccharide by enzyme catalysis and fermentation with kluyveromyces marxianus.	of three beta-galactosidases from aspergillus oryzae, kluyveromyces lactis and bacillus sp., used for the production of low-content galacto- oligosaccharides (gos) from lactose, the latter produced the highest yield of trisaccharides and tetrasaccharides. gos production was enhanced by mixing beta-galactosidase glucose oxidase. the low-content gos syrups, produced either by beta-galactosidase alone or by the mixed enzyme system, were subjected to the fermentation by kluyveromyces marxianus, wher ...	2006	16786243
an enzymatically produced novel cyclomaltopentaose cyclized from amylose by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}.	a bacterial strain am7, isolated from soil and identified as bacillus circulans, produced two kinds of novel cyclic oligosaccharides. the cyclic oligosaccharides were produced from amylose using a culture supernatant of the strain as the enzyme preparation. the major product was a cyclomaltopentaose cyclized by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}. the other minor product was cyclomaltohexao ...	2006	16545346
square-plate culture method allows detection of differential gene expression and screening of novel, region-specific genes in aspergillus oryzae.	when grown on solid agar medium, the mycelium of a filamentous fungus, aspergillus oryzae, forms three morphologically distinct regions: the tip (t), white (w), and basal (b) regions. in this study, we developed the square-plate culture method, a novel culture method that enabled the extraction of mrna samples from the three regions and analyzed the differential gene expression of the a. oryzae mycelium in concert with the microarray technique. expression of genes involved in protein synthesis w ...	2006	16708193
extracellular production of neoculin, a sweet-tasting heterodimeric protein with taste-modifying activity, by aspergillus oryzae.	neoculin (ncl), a protein with sweetness approximately 500-fold that of sugar, can be utilized as a nonglycemic sweetener. it also has taste-modifying activity to convert sourness to sweetness. ncl is a heterodimer composed of an n-glycosylated acidic subunit (nas) and a basic subunit (nbs), which are conjugated by disulfide bonds. for the production of recombinant ncl (rncl) by aspergillus oryzae, alpha-amylase with a kex2 cleavage site, -k-r-, was fused upstream of each of nas and nbs and the ...	2006	16672522
rate of iodine volatilization and accumulation by filamentous fungi through laboratory cultures.	five strains of basidiomycetes (lentinula edodes, coprinus phlyctidosporus, hebeloma vinosophyllum, pleurotus ostreatus and agaricus bisporus), one strain of ascomycete (hormoconis resinae) and six strains of imperfect fungi (penicillium chrysogenum, penicillium roquefortii, cladosporium cladosporioides, alternaria alternata, aspergillus niger and aspergillus oryzae) were cultured in a liquid medium containing a radioactive iodine tracer ((125)i), and were tested for their abilities to volatiliz ...	2006	16828143
vacuolar membrane dynamics in the filamentous fungus aspergillus oryzae.	vacuoles in filamentous fungi are highly pleomorphic and some of them, e.g., tubular vacuoles, are implicated in intra- and intercellular transport. in this report, we isolated aovam3, the homologue of the saccharomyces cerevisiae vam3 gene that encodes the vacuolar syntaxin, from aspergillus oryzae. in yeast complementation analyses, the expression of aovam3 restored the phenotypes of both deltavam3 and deltapep12 mutants, suggesting that aovam3p is likely the vacuolar and/or endosomal syntaxin ...	2006	16467481
aspergillus oryzae strains with a large deletion of the aflatoxin biosynthetic homologous gene cluster differentiated by chromosomal breakage.	recently we divided aspergillus oryzae rib strains into group 1, having seven aflatoxin biosynthesis homologous genes (aflt, nor-1, aflr, nora, avna, verb, and vbs), and group 2, having three homologues (avna, verb, and vbs). here, partial aflatoxin homologous gene cluster of rib62 from group 2 was sequenced and compared with that of rib40 from group 1. rib62 showed a large deletion upstream of ver-1 with more than half of the aflatoxin homologous gene cluster missing including aflr, a positive ...	2006	16673111
deletion analysis of the superoxide dismutase (sodm) promoter from aspergillus oryzae.	the manganese superoxide dismutase gene (sodm) is very highly expressed in aspergillus oryzae. to elucidate the basis for this high-level expression, deletion analysis of the promoter was undertaken using beta-glucuronidase (gus) as a reporter. deletion of a 63-bp sequence from -200 to -138 in the 1,038-bp sodm promoter caused a drastic decrease in gus activity. in addition, an electrophoretic gel mobility shift assay (emsa) implicated a 30-bp element from -209 to -178 containing cis-element(s) ...	2006	16547700
suppression on the mutagenicity of 4-nitroquinoline-n-oxide by the methanol extracts of soybean koji prepared with various filamentous fungi.	in this study, solid fermentation of soybean with various gras filamentous fungi including aspergillus sojae bcrc 30103, aspergillus oryzae bcrc 30222, aspergillus awamori, actinomucor taiwanesis and rhizopus sp. was performed to prepare various soybean kojis. toxicity, mutagenicity and suppression on the mutagenesis induced by a direct mutagen, 4-nitroquinoline-n-oxide (4-nqo) on salmonella typhimurium ta 100, by the various methanol extracts of the prepared soybean koji and unfermented soybean ...	2006	16580082
[production of antitumor substances from shochu distillation remnants].	this study aimed to develop a new processing method for the effective use of rice shochu distillation remnants. we examined the inhibitory effects on the growth of human lung carcinoma cells in the medium of rice shochu distillation remnants with various fungi. interestingly, high inhibitory effects on the growth of human lung carcinoma cells in the medium of rice shochu distillation remnants with aspergillus oryzae were obtained, although no inhibitory effect was observed in the case of synthet ...	2006	16679744
enhanced gene targeting frequency in ku70 and ku80 disruption mutants of aspergillus sojae and aspergillus oryzae.	in the koji molds aspergillus sojae and aspergillus oryzae, exogenous dna is integrated in the genome, in most cases irrespective of the sequence homology, suggesting that dna integration occurs predominantly through a nonhomologous end joining pathway where two ku genes, namely, ku70 and ku80, play a key role. to determine the effect of ku gene disruption on the gene targeting frequency, we constructed ku70-, ku80-, and ku70-ku80-disrupted strains of a. sojae and a. oryzae. the gene targeting f ...	2006	16470383
functional expression of candida antarctica lipase b in eschericha coli.	candida antarctica lipase b (calb) is an important catalyst in bio-organic synthesis. to optimize its performance, either the reaction medium is changed or the lipase itself is modified. in the latter case, mutants are generated in eschericha coli and subsequently expressed in fungal hosts for their characterization. here we present the functional expression of calb in the periplasm of e. coli. by step-wise deletion of the calb signal and propeptide we were able to express and purify two differe ...	2006	16713003
distribution profiles of isoflavone isomers in black bean kojis prepared with various filamentous fungi.	this study was conducted to compare the transformation of both isoflavone derivatives (aglycones, beta-glucosides, and acetyl and malonyl gluclucosides) and beta-glucosidase activity in kojis fermented with various generally recognized as safe filamentous fungi including aspergillus awamori, aspergillus oryzae, aspergillus sojae, rhizopus azygosporus, and rhizopus sp. no. 2. solid fermentation was performed to prepare the kojis by inoculating the steamed black beans with starter organism and cul ...	2006	16478253
improvement of the glab promoter expressed in solid-state fermentation (ssf) of aspergillus oryzae.	the glucoamylase-encoding gene (glab) promoter should be very useful for recombinant protein production in solid-state fermentation (ssf) of aspergillus oryzae. a 97-bp fragment containing the cis-element of the glab promoter was inserted into the glaa promoter, which was little expressed in ssf. the chimeric promoter showed about a 24-fold increase in promoter activity in ssf. eight copies of the 97-bp fragment were tandemly fused with the glab promoter. the improved promoter showed about a 4.6 ...	2006	16717420
novel hydrophobic surface binding protein, hsba, produced by aspergillus oryzae.	hydrophobic surface binding protein a (hsba) is a secreted protein (14.5 kda) isolated from the culture broth of aspergillus oryzae rib40 grown in a medium containing polybutylene succinate-co-adipate (pbsa) as a sole carbon source. we purified hsba from the culture broth and determined its n-terminal amino acid sequence. we found a dna sequence encoding a protein whose n terminus matched that of purified hsba in the a. ozyzae genomic sequence. we cloned the hsba genomic dna and cdna from a. ory ...	2006	16597938
effects of dietary oyster extract on lipid metabolism, blood pressure, and blood glucose in sd rats, hypertensive rats, and diabetic rats.	oyster extract was prepared by hydrolysis of oyster protein with proteases, aloase (a protease from bacillus subtilis), and pancitase (a protease from aspergillus oryzae). rats were fed a diet containing 20% casein (the control diet) or 15% casein and 5% oyster extract (the oyster extract diet) as the protein source. the oyster extract diet exerted a significant reduction in serum cholesterol and liver triglyceride concentrations as compared with the control diet in sprague-dawley (sd) rats fed ...	2006	16495664
[genome sequencing of aspergillus oryzae].		2006	16686348
cloning and expression of 1,2-alpha-mannosidase gene (fmanib) from filamentous fungus aspergillus oryzae: in vivo visualization of the fmanibp-gfp fusion protein.	1,2-alpha-mannosidase catalyzes the specific cleavage of 1,2-alpha-mannose residues from protein-linked n-glycan. in this study, a novel dna sequence homologous to the authentic 1,2-alpha-mannosidase was cloned from a cdna library prepared from solid-state cultured aspergillus oryzae. the fmanib cdna consisted of 1530 nucleotides and encoded a protein of 510 amino acids in which all consensus motifs of the class i alpha-mannosidase were conserved. expression of the full length of 1,2-alpha-manno ...	2006	16495665
comparison of the aflr gene sequences of strains in aspergillus section flavi.	aflatoxins are polyketide-derived secondary metabolites produced by aspergillus parasiticus, aspergillus flavus, aspergillus nomius and a few other species. the toxic effects of aflatoxins have adverse consequences for human health and agricultural economics. the aflr gene, a regulatory gene for aflatoxin biosynthesis, encodes a protein containing a zinc-finger dna-binding motif. although aspergillus oryzae and aspergillus sojae, which are used in fermented foods and in ingredient manufacture, h ...	2006	16385126
molecular analysis of an inactive aflatoxin biosynthesis gene cluster in aspergillus oryzae rib strains.	to help assess the potential for aflatoxin production by aspergillus oryzae, the structure of an aflatoxin biosynthesis gene homolog cluster in a. oryzae rib 40 was analyzed. although most genes in the corresponding cluster exhibited from 97 to 99% similarity to those of aspergillus flavus, three genes shared 93% similarity or less. a 257-bp deletion in the aflt region, a frameshift mutation in nora, and a base pair substitution in vera were found in a. oryzae rib 40. in the aflr promoter, two s ...	2006	16391082
direct assay for alpha-amylase using fluorophore-modified cyclodextrins.	a simple assay method for alpha-amylase was developed based on fluorophore-modified cyclodextrins (cds). four kinds of cd derivatives bearing a 4-amino-7-nitrobenz-2-oxa-1,3-diazole (nbd-amine) moiety were prepared as artificial substrates for the assay method. the fluorescence intensity of all the nbd-amine-modified cds decreased upon addition of aspergillus oryzae alpha-amylase, indicating a reduction in hydrophobicity near the nbd-amine moiety induced by hydrolysis of the cd ring. nc4gammacd, ...	2006	16464599
g-protein and camp-mediated signaling in aspergilli: a genomic perspective.	we have carried out an in silico exploration of the genomes of aspergillus nidulans, aspergillus fumigatus, and aspergillus oryzae, and identified components of g-protein/camp-mediated signaling. putative g-protein coupled receptors (gpcrs) were distributed over nine classes. the gpcrs within classes were well conserved among aspergilli but varied in other ascomycetes. as previously observed in a. nidulans and other fungi, three galpha, one gbeta, and one ggamma subunits of g proteins were ident ...	2006	16546420
enhancement of enantioselectivity in the bacillus subtilis protease-catalyzed hydrolysis of n-free amino acid esters using the ester grouping-modification approach.	the generality of enantioselectivity enhancement through the modification of the alcohol moiety of a substrate ester was ascertained, for in the bacillus subtilis protease-catalyzed hydrolysis of n-unprotected amino acid esters the enantioselectivity was enhanced largely by switching the conventional methyl ester to esters with a longer alkyl chain such as the isobutyl ester (from e = 3 to e = 130-170 in the case of 4-fluorophenylalanine esters) as in the enzymatic hydrolysis mediated by aspergi ...	2006	16614915
the region in a subunit of the aspergillus ccaat-binding protein similar to the hap4p-recruiting domain of saccharomyces cerevisiae hap5p is not essential for transcriptional enhancement.	the ccaat-binding complex in aspergillus species, known as the hap complex, consists of at least three subunits, hapb, hapc, and hape. each hap subunit contains an evolutionarily conserved core domain. in this study, a series of the truncated gene, which encodes the hape subunit of aspergillus oryzae, was constructed to survey the regions essential for the transcriptional enhancement of fungal genes. it was revealed that the non-conserved regions and the conserved region similar to the hap4p rec ...	2006	16636442
expression of aspergillus hemoglobin domain activities in aspergillus oryzae grown on solid substrates improves growth rate and enzyme production.	dna fragments coding for hemoglobin domains (hbd) were isolated from aspergillus oryzae and aspergillus niger. the hbd activities were expressed in a. oryzae by introduction of hbd gene fragments under the control of the promoter of the constitutively expressed gpda gene. in the transformants, oxygen uptake was significantly higher, and during growth on solid substrates the developed biomass was at least 1.3 times higher than that of the untransformed wild-type strain. growth rate of the hbd-act ...	2006	16927259
enhanced antioxidative activity of soybean koji prepared with various filamentous fungi.	in the present study, soybean koji fermented with various gras filamentous fungi, including aspergillus sojae bcrc 30103, aspergillus oryzae bcrc 30222, aspergillus awamori, actinomucor taiwanensis and rhizopus sp. these organisms are commonly used as starters in the fermentation of many traditional, oriental food products. the growth of starter organisms, total phenolic content, and antioxidative activities of the methanol extract of these kojis are compared with specific reference to alpha-dip ...	2006	16943061
a novel gh43 alpha-l-arabinofuranosidase from humicola insolens: mode of action and synergy with gh51 alpha-l-arabinofuranosidases on wheat arabinoxylan.	a novel alpha-l-arabinofuranosidase (alpha-araf) belonging to glycoside hydrolase (gh) family 43 was cloned from humicola insolens and expressed in aspergillus oryzae. (1)h-nmr analysis revealed that the novel gh43 enzyme selectively hydrolysed (1-->3)-alpha-l-arabinofuranosyl residues of doubly substituted xylopyranosyl residues in arabinoxylan and in arabinoxylan-derived oligosaccharides. the optimal activity of the cloned enzyme was at ph 6.7 and 53 degrees c. two other novel alpha-l-arabinof ...	2006	16944135
impact of aspergillus oryzae genomics on industrial production of metabolites.	aspergillus oryzae is used extensively for the production of the traditional japanese fermented foods sake (rice wine), shoyu (soy sauce), and miso (soybean paste). in recent years, recombinant dna technology has been used to enhance industrial enzyme production by a. oryzae. recently completed genomic studies using expressed sequence tag (est) analyses and whole-genome sequencing are quickly expanding the industrial potential of the fungus in biotechnology. genes that have been newly discovered ...	2006	16944282
identification of genes from aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture.	we identified 22 genes from aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture (mslc), among which ser/thr protein kinase (aopk1) and phosphatase (aoppt) genes were cloned. we also revealed that aopk1 encodes a protein with an n-terminal sequence 150 amino acid residues longer than that predicted from the registered sequence in genbank.	2006	17189178
first report of a xylose-specific lectin with potent hemagglutinating, antiproliferative and anti-mitogenic activities from a wild ascomycete mushroom.	from fresh fruiting bodies of the wild ascomycete mushroom (xylaria hypoxylon) a lectin with n-terminal sequence resemblance to a part of aspergillus oryzae genome and only slight similarity to fungal immunomodulatory protein from the mushroom flammulina velutipes was isolated. the protocol comprised extraction with water, precipitation from the aqueous extract using 80% saturated (nh(4))(2)so(4), ion exchange chromatography on deae-cellulose and cm-cellulose, and then gel filtration by fast pro ...	2006	16952421
a traditional japanese medicine mitigates tnbs-induced colitis in rats.	differences in dietary habits may be one of the reasons that the incidence of inflammatory bowel disease has remained lower in japan than in western countries. we investigated whether a traditional japanese medicine (strong wakamto), based on aspergillus oryzae koji, would exert any effect on experimental colitis in rats.	2006	16990203
innovative effect of illite on improved microbiological conversion of l-tyrosine to 3,4 dihydroxy phenyl l-alanine (l-dopa) by aspergillus oryzae me2 under acidic reaction conditions.	in the present investigation, the previous ultraviolet irradiated mutant strain of aspergillus oryzae uv-7 was further improved in terms of 3,4 dihydroxy phenyl l-alanine (l-dopa) activity after chemical mutagenesis through 1-methyl 3-nitro 1-nitroso guanidine (mnng = 250-1500 microg/ml) treatment (0-30 min). among several mutant variants, the one that produced a larger amount of l-dopa from l-tyrosine was designated to as me2 and it was made 2-deoxy-d-glucose-resistant by growing it at various ...	2006	17039388
a new mechanism regulating the initiation of allergic airway inflammation.	the earliest immune events induced by allergens are poorly understood, yet are likely essential to understanding how allergic inflammation is established.	2007	17544098
effects of a dietary aspergillus oryzae extract containing alpha-amylase activity on performance and carcass characteristics of finishing beef cattle.	three experiments were conducted to examine the effects of an aspergillus oryzae extract containing alpha-amylase activity on performance and carcass characteristics of finishing beef cattle. in exp. 1, 120 crossbred steers were used in a randomized complete block design to evaluate the effects of roughage source (alfalfa hay vs. cottonseed hulls) and supplemental alpha-amylase at 950 dextrinizing units (du)/kg of dm. significant roughage source x alpha-amylase interactions (p < 0.05) were obser ...	2007	17121970
substrate specificity of n-acetylhexosaminidase from aspergillus oryzae to artificial glycosyl acceptors having various substituents at the reducing ends.	the substrate specificity of n-acetylhexosaminidase (e.c. 3.2.1.51) from aspergillus oryzae was examined using p-nitrophenyl 6-o-sulfo-n-acetyl-beta-d-glucosaminide (6-o-sulfo-glcnac-o-pnp) as the glycosyl donor and a series of beta-d-glucopyranosides and n-acetyl-beta-d-glucosaminides with variable aglycons at the anomeric positions as the acceptors. when beta-d-glucopyranosides with methyl (ch(3)), allyl (ch(2)chch(2)), and phenyl (c(6)h(5)) groups at the reducing end were used as the acceptor ...	2007	17145046
fungal strains isolated from cork stoppers and the formation of 2,4,6-trichloroanisole involved in the cork taint of wine.	cork taint is mainly due to 2,4,6-trichloroanisole (tca) produced through the activity of undesirable fungal strains. we observed that cfu mould number in tca-containing stoppers was not quantitatively different to that of the stoppers not containing tca (ca. 10(5)cfu/g). in contrast more fungi diversity was observed in tca-containing stoppers. penicillium spp (penicillium chrysogenum, penicillium glabrum), aspergillus spp (aspergillus niger and aspergillus oryzae), chrysonilia sitophila, mucor ...	2007	17188205
the promoter activity of isovaleryl-coa dehydrogenase-encoding gene (ivda) from aspergillus oryzae is strictly repressed by glutamic acid.	we cloned the isovaleryl-coa dehydrogenase (ivd)-encoding gene from aspergillus oryzae. the promoter of ivda was subjected to beta-glucuronidase (gus) reporter assays in which certain amino acids were used as a major carbon source. l-leucine most strongly induced gus-activity, while in the case of l-glutamate, significantly low activity was found, indicating that ivda transcription was strongly repressed by glutamic acid.	2007	17587691
calcium alginate entrapped preparations of aspergillus oryzae beta galactosidase: its stability and applications in the hydrolysis of lactose.	insoluble concanavalin a-beta galactosidase complex was obtained by using jack bean extract and this complex was crosslinked with glutaraldehyde, in order to maintain the integrity of complex in the presence of its substrate or products. concanavalin a-beta galactosidase complex retained 92% of the initial enzyme activity whereas crosslinked complex showed 88% activity. entrapment of concanavalin a-beta galactosidase complex into calcium alginate beads provided suitability to use this preparatio ...	2007	17298841
temperature impacts the multiple attack action of amylases.	the action pattern of several amylases was studied at 35, 50, and 70 degrees c using potato amylose, a soluble (red starch) and insoluble (cross-linked amylose) chromophoric substrate. with potato amylose as substrate, bacillus stearothermophilus alpha-amylase (bsta) and porcine pancreatic alpha-amylase displayed a high degree of multiple attack (dma, i.e., the number of bonds broken during the lifetime of an enzyme-substrate complex minus one), the fungal alpha-amylase from aspergillus oryzae a ...	2007	17309295
biotransformation of piceid in polygonum cuspidatum to resveratrol by aspergillus oryzae.	biotransformation of piceid in polygonum cuspidatum to resveratrol by aspergillus oryzae was investigated in this study. resveratrol is widely used in medicine, food, and cosmetic because of its pharmacological properties. however, it has a much lower content in plants compared with its glucoside piceid, which has a much lower bioavailability. traditionally, the aglycone is acquired by acid or enzymatic hydrolysis of its glucoside, but the violent condition and the acid pollution in hydrolytic r ...	2007	17333175
the glucoamylase-encoding gene (glab) is expressed in solid-state culture with a low water content.	solid-state culture encourages high-level enzyme secretion by aspergillus oryzae. using the real-time quantitative reverse transcriptase-polymerase chain reaction, we confirmed that expression of the glucoamylase-encoding gene in a. oryzae cultured in solid-state culture depends on the water content of the culture.	2007	17617703
chemopreventive effect of fermented brown rice and rice bran on 4-nitroquinoline 1-oxide-induced oral carcinogenesis in rats.	the preventive effects of the dietary administration of brown rice and rice bran fermented with aspergillus oryzae (fbra) on oral carcinogenesis induced by 4-nitroquinoline 1-oxide (4-nqo) were investigated in male f344 rats. at 7 weeks of age, the animals were given 20 ppm 4-nqo in their drinking water for 8 weeks to induce tongue neoplasms. groups of rats were fed diets containing 5 or 10% fbra during the initiation or postinitiation phases of the 4-nqo-induced oral carcinogenesis. the other g ...	2007	17342331
profiling of glycosidase activities using coumarin-conjugated glycoside cocktails.	glycosidases are a large subgroup of carbohydrate-processing enzymes that hydrolytically cleave the glycosidic bond. glycans formed by the action of glycosidases are involved in various biological processes. genetic abnormalities in glycosidases are associated with inherited diseases. thus, characterization of the catalytic activities of glycosidases is of great importance. herein, we describe a simple and rapid approach for determining glycosidase activity profiles using coumarin-conjugated gly ...	2007	17256948
mutagenic and antimutagenic effects of methanol extracts of unfermented and fermented black soybeans.	in this study, solid fermentation of steamed black soybean with various gras (generally recognized as safe) filamentious-fungi including aspergillus awamori, aspergillus oryzae bcrc 30222, aspergillus sojae bcrc 30103, rhizopus azygosporus bcrc 31158 and rhizopus sp. no. 2 was performed. mutagenicity and antimutagenicity of the methanol extracts of unfermented and fermented steamed black soybeans against 4-nitroquinoline-n-oxide (4-nqo), a direct mutagen and benzo[a]pyrene (b[a]p), an indirect m ...	2007	17628128
molecular cloning, and characterization of a modular acetyl xylan esterase from the edible straw mushroom volvariella volvacea.	a new volvariella volvacea gene encoding an acetyl xylan esterase (designated as vvaxe1) was cloned and expressed in pichia pastoris. the cdna contained an orf of 1047 bp encoding 349 amino acids with a calculated mass of 39 990 da. vvaxe1 is a modular enzyme consisting of an n-terminal signal peptide, a catalytic domain, and a cellulose-binding domain. the amino acid sequence of the enzyme exhibited a high degree of similarity to cinnamoyl esterase b from penicillium funiculosum, and acetyl xyl ...	2007	17623028
development of an efficient production method for beta-mannosidase by the creation of an overexpression system in aspergillus aculeatus.	to develop an overexpression system in aspergillus aculeatus in order to establish an efficient overproduction method of beta-mannosidase (manb).	2007	17651209
light represses conidiation in koji mold aspergillus oryzae.	in the filamentous fungus aspergillus oryzae, there has been no report on photoreaction. here we investigated the effect of light in a. oryzae and found that conidiation was repressed by white light. this reaction is contrary to that of other aspergilli, which show abundant conidiation under light. moreover, red light also caused reduced conidiation. genome sequencing of a. oryzae indicated the existence of homologs of some light-related genes in other filamentous fungi. to approach the molecula ...	2007	17690479
[expression of endopolygalacturonase a of aspergillus oryzae in escherichia coli].	pectinases are mainly used in the food industry to clarify fruit juices and wine, improve oil extraction, remove the peel from the citrus fruit, increase the firmness of some fruits and degum fibres. the filamentous fungus aspergillus oryzae, used for the production of traditional fermented foods, only could produce less pectinases under general conditions. so far only a few of pgs expressed in yeast or e. coli were reported but they did not show higher activity. the cdna of mature pga (without ...	2007	17366896
production of dye-decolorizing peroxidase (rdyp) from complex substrates by repeated-batch and fed-batch cultures of recombinant aspergillus oryzae.	we examined production levels of dye-decolorizing peroxidase (rdyp) by recombinant aspergillus oryzae using wheat bran and rice bran powders in repeated-batch and fed-batch cultures. similar average rdyp productivities were observed in repeated-batch cultures using wheat bran powder and rice bran powder. average rdyp productivities in fed-batch cultures were slightly lower than those in repeated-batch cultures. the rdyp production was affected by the addition of k(2)hpo(4) in the repeated-batch ...	2007	17368394
screening of beta-fructofuranosidase-producing microorganisms and effect of ph and temperature on enzymatic rate.	seventeen different strains of filamentous fungi were grown in batch cultures to compare their abilities for the production of beta-fructofuranosidase. three of them, aspergillus oryzae ipt-301, aspergillus niger atcc 20611 and strain ipt-615, showed high production with total fructosyltransferase activity higher than 12,500 units l(-1). in addition, the beta-fructofuranosidases of those strains have a high fructosyltransferase activity-to-hydrolytic activity ratio. the temperature and ph effect ...	2007	17375295
carbohydrate binding specificity of a fucose-specific lectin from aspergillus oryzae: a novel probe for core fucose.	the alpha1,6-fucosyl residue (core fucose) of glycoproteins is widely distributed in mammalian tissues and is altered under pathological conditions. a probe that specifically detects core fucose is important for understanding the role of this oligosaccharide structure. aleuria aurantia lectin (aal) and lens culimaris agglutinin-a (lca) have been often used as carbohydrate probes for core fucose in glycoproteins. here we show, by using surface plasmon resonance (spr) analysis, that aspergillus or ...	2007	17383961
the beta-1,3-exoglucanase gene exga (exg1) of aspergillus oryzae is required to catabolize extracellular glucan, and is induced in growth on a solid surface.	the biological role of exga (exg1), a secretory beta-1,3-exoglucanase of aspergillus oryzae, and the expression pattern of the exga (exg1) gene were analyzed. the exga disruptant and the exga-overexpressing mutant were constructed, and phenotypes of both mutants were compared. higher mycelial growth rate and conidiation efficiency were observed for the exga-overexpressing mutant than for the exga disruptant when beta-1,3-glucan was supplied as sole carbon source. on the other hand, no difference ...	2007	17420593
phosphorylation of the aspergillus oryzae woronin body protein, aohex1, by protein kinase c: evidence for its role in the multimerization and proper localization of the woronin body protein.	woronin body, a specialized peroxisome, is a unique organelle involved in septal pore sealing and protecting filamentous fungus from excessive cytoplasmic bleeding. we recently characterized the aohex1 gene encoding the major protein of the woronin body in the fungus aspergillus oryzae. although three-dimensional microscopy revealed plugging of the septal pore by woronin body, the mechanism of its formation remains unknown. we report here a reduction in the oligomeric forms (dimeric and tetramer ...	2007	17441786
microbial production of sensory-active miraculin.	miraculin (mcl), a tropical fruit protein, is unique in that it has taste-modifying activity to convert sourness to sweetness, though flat in taste at neutral ph. to obtain a sufficient amount of mcl to examine the mechanism involved in this sensory event at the molecular level, we transformed aspergillus oryzae by introducing the mcl gene. transformants were expressed and secreted a sensory-active form of mcl yielding 2 mg/l. recombinant mcl resembled native mcl in the secondary structure and t ...	2007	17592723
enhancement of substrate recognition ability by combinatorial mutation of beta-glucosidase displayed on the yeast cell surface.	recently, in family 3 beta-glucosidase (bgl), the catalytically important asp nucleophile has been identified in the sdw segment of the sdwg sequence by site-directed mutagenesis. however, the details about the roles of each amino acid residue of the sdwg sequence have not been investigated. w293 of the sdw segment, which is the residue next to the nucleophile (d292) in family 3 bgl, is very important for hydrolytic reaction as a binder to a substrate. g294 of the sdwg sequence might play an imp ...	2007	17602218
fungal-stressed germination of black soybeans leads to generation of oxooctadecadienoic acids in addition to glyceollins.	microbial-stressed germination of black soybeans leads to generation of a group of oxylipins, oxooctadecadienoic acids (kodes, including 13- z, e-kode, 13- e, e-kode, 9- e, z-kode, and 9- e, e-kode), and their respective glyceryl esters in addition to glyceollins, a known phytoalexins present in wild and fungi-infected soybeans. four fungi, aspergillus niger, aspergillus oryzae, rhizopus oligosporus, and white rice yeast ( aspergillus niger wry), were applied to compare their efficiency on induc ...	2007	17892258
fermentation as a bio-process to obtain functional soybean flours.	the effect of fermentation on the antioxidant compounds [vitamins c and e, total phenolic compounds (tpc), and reduced glutathione (gsh)], and antioxidant capacity [superoxide anion scavenging activity (sod-like activity), peroxyl radical-trapping capacity (prtc), inhibition of phosphatidylcholine (pc) peroxidation, and trolox equivalent antioxidant capacity (teac)] of soybean (glycine max cv. merit) was studied. fermentation was carried out in solid state in cracked seeds inoculated with asperg ...	2007	17907774
double disruption of the proteinase genes, tppa and pepe, increases the production level of human lysozyme by aspergillus oryzae.	in this study, we investigated the effects of proteinase gene disruption on heterologous protein production by aspergillus oryzae. the human lysozyme (hly) was selected for recombinant production as a model for the heterologous protein. a tandem hly construct fused with alpha-amylase (amyb) was expressed by a. oryzae in which the kex2 cleavage site was inserted at the upstream of hly. hly was successfully processed from amyb and produced in the medium. we performed a systematic disruption analys ...	2007	17622525
individual and combined effects of physicochemical parameters on ellagitannin acyl hydrolase and ellagic acid production from ellagitannin by aspergillus oryzae.	the individual and interactive effects of physicochemical parameters on ellagitannin acyl hydrolase activity and ellagic acid production by aspergillus oryzae using ellagitannins from acorn fringe of oak as substrate were studied. ellagitannins concentration, incubation time were identified as important physicochemical parameters influencing the enzyme synthesis and the production accumulation, and the substrate concentration with initial ph was determined to has an interactive effect on the enz ...	2007	17450459
marked production of ginsenosides rd, f2, rg3, and compound k by enzymatic method.	the hydrolysis of protopanaxadiol-type saponin mixture by various glycoside hydrolases was examined. among these enzymes, crude preparations of lactase from aspergillus oryzae, beta-galactosidase from a. oryzae, and cellulase from trichoderma viride were found to produce ginsenoside f(2) [3-o-(beta-d-glucopyranosyl)-20-o-beta-d-glucopyranosyl-20(s)-protopanaxadiol], compound k [20-o-beta-d-glucopyranosyl-20(s)-protopanaxadiol], and ginsenoside rd {3-o-[beta-d-glucopyranosyl-(1-->2)-beta-d-glucop ...	2007	17917300
aspergillus oryzae peritonitis in capd: case report and review of the literature.	fungal peritonitis is a rare, but serious, complication of continuous ambulatory peritoneal dialysis (capd). we report a case of peritonitis caused by aspergillus oryzae in a man on capd therapy who was treated successfully with amphotericin b and caspofungin, followed by itraconazole and removal of the peritoneal catheter. a oryzae was identified by using sequence analysis of the ribosomal dna genes. of 10 reported cases since 2003, the mortality rate was 30%. removal of the capd catheter and s ...	2007	17472853
tpr domain of ser/thr phosphatase of aspergillus oryzae shows no auto-inhibitory effect on the dephosphorylation activity.	a ser/thr phosphatase gene cloned from aspergillus oryzae, aoppt, revealed that the tetratricopeptide repeat (tpr) and catalytic domains of the full-length aoppt are located at the n- and c-terminal regions, respectively, similar to those of human ser/thr phosphatase 5 (pp5) and yeast ppt1. four different regions of aoppt, namely, a full-length polypeptide, the catalytic domain, the catalytic domain plus c-terminal 15 amino-acid residues and the tpr domain were expressed in escherichia coli and ...	2007	17475320
genomics of aspergillus oryzae.	the genome sequence of aspergillus oryzae, a fungus used in the production of the traditional japanese fermentation foods sake (rice wine), shoyu (soy sauce), and miso (soybean paste), has revealed prominent features in its gene composition as compared to those of saccharomyces cerevisiae and neurospora crassa. the a. oryzae genome is extremely enriched with genes involved in biomass degradation, primary and secondary metabolism, transcriptional regulation, and cell signaling. even compared to t ...	2007	17341818
gene silencing by rna interference in the koji mold aspergillus oryzae.	we found the orthologous genes required for rna interference (rnai) in the aspergillus oryzae genome database, and constructed a set of tools for gene silencing using rnai in a. oryzae. this system utilizes compatible restriction enzyme sites so that only a single target gene fragment is required to create the hairpin rna cassette. for ease of handling, we also separated the construction of the hairpin rna cassette for the target gene from its subsequent introduction into the expression vector. ...	2007	17213655
change in turnover capacity of crude recombinant dye-decolorizing peroxidase (rdyp) in batch and fed-batch decolorization of remazol brilliant blue r.	decolorization of the representative anthraquinone dye, remazol brilliant blue r (rbbr) was assessed to determine the practical potential of crude recombinant dye-decolorizing peroxidase generated by aspergillus oryzae (rdyp) in term of turnover capacity of rdyp. the turnover capacity, defined as the milligram of rbbr decolorized per unit of rdyp inactivated over the catalytic life time of rdyp, was quantified under condition by h(2)o(2) -mediated rdyp inactivation. in batch culture, equimolar b ...	2007	17569040
comparative profiles of alpha-amylase production in conventional tray reactor and growtek bioreactor.	growtek bioreactor was used as modified solid-state fermentor to circumvent many of the problems associated with the conventional tray reactors for solid-state fermentation (ssf). aspergillus oryzae ifo-30103 produced very high levels of alpha-amylase by modified solid-state fermentation (mssf) compared to ssf carried out in enamel coated metallic trays utilizing wheat bran as substrate. high alpha-amylase yield of 15,833 u g(-1) dry solid in mssf were obtained when the fungus were cultivated at ...	2007	17573554
structure of the dimeric n-glycosylated form of fungal beta-n-acetylhexosaminidase revealed by computer modeling, vibrational spectroscopy, and biochemical studies.	fungal beta-n-acetylhexosaminidases catalyze the hydrolysis of chitobiose into its constituent monosaccharides. these enzymes are physiologically important during the life cycle of the fungus for the formation of septa, germ tubes and fruit-bodies. crystal structures are known for two monomeric bacterial enzymes and the dimeric human lysosomal beta-n-acetylhexosaminidase. the fungal beta-n-acetylhexosaminidases are robust enzymes commonly used in chemoenzymatic syntheses of oligosaccharides. the ...	2007	17509134
aspergillus flavus: human pathogen, allergen and mycotoxin producer.	aspergillus infections have grown in importance in the last years. however, most of the studies have focused on aspergillus fumigatus, the most prevalent species in the genus. in certain locales and hospitals, aspergillus flavus is more common in air than a. fumigatus, for unclear reasons. after a. fumigatus, a. flavus is the second leading cause of invasive aspergillosis and it is the most common cause of superficial infection. experimental invasive infections in mice show a. flavus to be 100-f ...	2007	17526826
analysis of expressed sequence tags from the fungus aspergillus oryzae cultured under different conditions.	we performed random sequencing of cdnas from nine biologically or industrially important cultures of the industrially valuable fungus aspergillus oryzae to obtain expressed sequence tags (ests). consequently, 21 446 raw ests were accumulated and subsequently assembled to 7589 non-redundant consensus sequences (contigs). among all contigs, 5491 (72.4%) were derived from only a particular culture. these included 4735 (62.4%) singletons, i.e. lone ests overlapping with no others. these data showed ...	2007	17540709
chemometric analysis of in-line multi-wavelength fluorescence measurements obtained during cultivations with a lipase producing aspergillus oryzae strain.	the filamentous fungus, aspergillus oryzae, was cultivated in batch and fed-batch cultivations in order to investigate the use of multi-wavelength fluorescence for monitoring course of events during filamentous fungi cultivations. the a. oryzae strain applied expressed a fungal lipase from thermomyces lanuginosus. spectra of multi-wavelength fluorescence were collected every 5 min with the bioview system (delta, denmark) and both explorative and predictive models, correlating the fluorescence da ...	2007	16948165
improvement of the antitumor activity of black currant polysaccharide by an enzymatic treatment.	a polysaccharide-rich substance isolated from black currant, named cassis polysaccharide (caps), was partially digested with beta-galactosidase from aspergillus oryzae and its immunostimulatory activity was investigated. the in vitro cytokine-inducing effect of caps on raw264 cells was gradually decreased along with lowering of the average mw of caps. in vivo, partially digested caps with a mean mw of approximately 20,000 showed the most potent antitumor activity against ehrlich carcinoma in mic ...	2007	17485844
synthesis of p-nitrophenyl sulfated disaccharides with beta-d-(6-sulfo)-glcnac units using beta-n-acetylhexosaminidase from aspergillus oryzae in a transglycosylation reaction.	when alpha-d-glcnac-oc(6)h(4)no(2) -p and beta-d-(6-sulfo)-glcnac-oc(6)h(4)no(2)-p (2) were used as substrates, beta-n-acetylhexosaminidase from aspergillus oryzae transferred the beta-d-(6-sulfo)-glcnac(unit from 2 to alpha-d-glcnac-oc(6)h(4)no(2) -p to afford beta-d-(6-sulfo)-glcnac-(1-->4)-alpha-d-glcnac-oc(6)h(4)no(2)-p (3) in a yield of 94% based on the amount of donor, 2, added. beta-d-(6-sulfo)-glcnac-(1-->4)-alpha-d-glc-oc(6)h(4)no(2)-p (4) was obtained with alpha-d-glc-oc(6)h(4)no(2) -p ...	2007	17492477
absence of mutagenicity, genotoxicity, and subchronic oral toxicity of touchi extract.	touchi, a traditional chinese food used mainly for seasoning is obtained by first steaming soybeans followed by fermentation with aspergillus oryzae (koji). a series of toxicological studies was conducted to evaluate the mutagenic and genotoxic potential and subchronic toxicity of a water extract of touchi, a known inhibitor of alpha -glucosidase activity. touchi extract (te) did not induce reverse mutations in salmonella typhimurium strains ta98, ta1537, ta100, ta1535, and escherichia coli wp2u ...	2007	17963133
effects of fermented soybean meal on digestive enzyme activities and intestinal morphology in broilers.	the experiment was performed to compare the effects of fermented soybean meal (fermented with aspergillus oryzae 3.042, fsbm) and soybean meal on digestive enzyme activities and intestinal morphology in broilers. three hundred twenty 1-d-old ross x ross male broiler chicks were randomly allocated into 2 dietary treatments for a 6-wk feeding trial, including 0- to 21-d and 21- to 42-d periods. at the end of each stage, 8 broilers of each treatment were killed, and pancreas, small intestine digest ...	2007	17495085
reversible and irreversible unfolding of multi-domain proteins.	in contrast to single-domain proteins unfolding of larger multi-domain proteins is often irreversible. in a comparative case study on three different multi-domain proteins (phosphoglycerate kinase: pgk and two homologous alpha-amylases: taka and bla) we investigated properties of unfolded states and their ability to fold back into the native state. for this purpose guanidine hydrochloride, alkaline ph, and thermal unfolded states were characterized. structural alterations upon unfolding and refo ...	2007	17964867
purification, characterization, and substrate specificity of a glucoamylase with steroidal saponin-rhamnosidase activity from curvularia lunata.	it has been previously reported that a glucoamylase from curvularia lunata is able to hydrolyze the terminal 1,2-linked rhamnosyl residues of sugar chains at c-3 position of steroidal saponins. in this work, the enzyme was isolated and identified after isolation and purification by column chromatography including gel filtration and ion-exchange chromatography. analysis of protein fragments by maldi-tof/tof proteomics analyzer indicated the enzyme to be 1,4-alpha-d-glucan glucohydrolase ec 3.2.1. ...	2007	17823796
modifying effects of fermented brown rice on fecal microbiota in rats.	brown rice fermented by aspergillus oryzae (fbra) is a fiber-rich food. effects of dietary administration of fbra on rat fecal microbiota composition were examined. male wistar rats were fed a basal diet or a 5% fbra- or 10% fbra-containing diet, and fecal microbiota was analyzed by culture and terminal-restriction fragment length polymorphism (t-rflp) analysis. the viable cell number of lactobacilli significantly increased after feeding 10% fbra diet for 3 weeks compared with that in the basal ...	2007	17826198
some distinguishable properties between acid-stable and neutral types of alpha-amylases from acid-producing koji.	the highly humid climate of japan facilitates the growth of various molds. among these molds, aspergillus oryzae is the most important and popular in japan, and has been used as yellow-koji in producing many traditional fermented beverages and foods, such as japanese sake, and soy sauce. taka-amylase a (taa), a major enzyme produced by the mold, is well known worldwide to be a leading enzyme for industrial utilization and academic study, since many extensive studies have been carried out with ta ...	2007	18086434
characterisation of 3-methylorcinaldehyde synthase (mos) in acremonium strictum: first observation of a reductive release mechanism during polyketide biosynthesis.	isolation and sequencing of a pks gene isolated from xenovulene-producing cultures of acremonium strictum indicated the presence of nt-, ks-, at-, pt-, c-met- and r-domains; heterologous expression in aspergillus oryzae resulted in the production of 3-methylorcinaldehyde, demonstrating the role of the terminal reductase domain in product release.	2007	17912413