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transcriptome analysis reveals crosstalk of responsive genes to multiple abiotic stresses in cotton (gossypium hirsutum l.).abiotic stress is a major environmental factor that limits cotton growth and yield, moreover, this problem has become more and more serious recently, as multiple stresses often occur simultaneously due to the global climate change and environmental pollution. in this study, we sought to identify genes involved in diverse stresses including abscisic acid (aba), cold, drought, salinity and alkalinity by comparative microarray analysis. our result showed that 5790, 3067, 5608, 778 and 6148 transcri ...201324224045
the dual functions of wlim1a in cell elongation and secondary wall formation in developing cotton fibers.lin-11, isl1 and mec-3 (lim)-domain proteins play pivotal roles in a variety of cellular processes in animals, but plant lim functions remain largely unexplored. here, we demonstrate dual roles of the wlim1a gene in fiber development in upland cotton (gossypium hirsutum). wlim1a is preferentially expressed during the elongation and secondary wall synthesis stages in developing fibers. overexpression of wlim1a in cotton led to significant changes in fiber length and secondary wall structure. comp ...201324220634
accumulation of biomass and mineral elements with calendar time by cotton: application of the expanded growth model.accumulation of plant biomass (mg ha(-1)) with calendar time (wk) occurs as a result of photosynthesis for green land-based plants. a corresponding accumulation of mineral elements (kg ha(-1)) such as nitrogen, phosphorus, and potassium occurs from the soil through plant roots. field data from literature for the warm-season annual cotton (gossypium hirsutum l.) are used in this analysis. the expanded growth model is used to describe accumulation of biomass and mineral elements with calendar time ...201324039802
isolation and expression profiling of ghnac transcription factor genes in cotton (gossypium hirsutum l.) during leaf senescence and in response to stresses.nac (nam, ataf, and cuc) is a plant-specific transcription factor family with diverse roles in plant development and stress regulation. in this report, stress-responsive nac genes (ghnac8-ghnac17) isolated from cotton (gossypium hirsutum l.) were characterised in the context of leaf senescence and stress tolerance. the characterisation of nac genes during leaf senescence has not yet been reported for cotton. based on the sequence characterisation, these ghnacs could be classified into three grou ...201324036432
functional analyses of cotton (gossypium hirsutum l.) immature fiber (im) mutant infer that fiber cell wall development is associated with stress responses.cotton fiber maturity is an important factor for determining the commercial value of cotton. how fiber cell wall development affects fiber maturity is not well understood. a comparison of fiber cross-sections showed that an immature fiber (im) mutant had lower fiber maturity than its near isogenic wild type, texas marker-1 (tm-1). the availability of the im mutant and tm-1 provides a unique way to determine molecular mechanisms regulating cotton fiber maturity.201324341782
harmonic radar tagging for tracking movement of nezara viridula (hemiptera: pentatomidae).harmonic radar tagging was investigated as a method for monitoring the movement of the southern green stink bug, nezara viridula (l.) (hemiptera: pentatomidae). because adhesive toxicity and tag weight limit the use of this technology, initial efforts focused on selection of the optimal adhesive and design of harmonic radar tags to reduce impact on the movement of stink bugs. a design consisting of a 6-cm-long 0.10-mm-thick silver-plated copper monopole on the anode terminal of a three-contact s ...201324331611
rna-seq transcriptome profiling of upland cotton (gossypium hirsutum l.) root tissue under water-deficit stress.an rna-seq experiment was performed using field grown well-watered and naturally rain fed cotton plants to identify differentially expressed transcripts under water-deficit stress. our work constitutes the first application of the newly published diploid d5 gossypium raimondii sequence in the study of tetraploid ad1 upland cotton rna-seq transcriptome analysis. a total of 1,530 transcripts were differentially expressed between well-watered and water-deficit stressed root tissues, in patterns tha ...201324324815
effects of fuzzless cottonseed phenotype on cottonseed nutrient composition in near isogenic cotton (gossypium hirsutum l.) mutant lines under well-watered and water stress conditions.there is no information available on the effect of fuzzless seed trait on cottonseed nutrient composition (minerals, n, s, protein, and oil) under drought stress. the objective of this research was to investigate the effect of the fuzzless seed trait on cottonseed nutrients using five sets of near-isogenic lines (nils). each set consists of two lines that share the same genetic background, but differ in seed fuzziness (fuzzy, f; fuzzless, n). the near isogenic lines will enable us to compare the ...201324416037
construction of microsatellite-based linkage map and mapping of nectarilessness and hairiness genes in gossypium tomentosum.gossypium tomentosum, a wild tetraploid cotton species with ad genomes, possesses genes conferring strong fibers and high heat tolerance. to effectively transfer these genes into gossypium hirsutum, an entire microsatellite (simple sequence repeat, ssr)-based genetic map was constructed using the interspecific cross of g. hirsutum x g. tomentosum (ht). we detected 1800 loci from 1347 pairs of polymorphic primers. of these, 1204 loci were grouped into 35 linkage groups at lod ≥ 4. the map covers ...201324371166
the promoter structure differentiation of a myb transcription factor rlc1 causes red leaf coloration in empire red leaf cotton under light.the red leaf coloration of empire red leaf cotton (erlc) (gossypium hirsutum l.), resulted from anthocyanin accumulation in light, is a well known dominant agricultural trait. however, the underpin molecular mechanism remains elusive. to explore this, we compared the molecular biological basis of anthocyanin accumulation in both erlc and the green leaf cotton variety ccri 24 (gossypium hirsutum l.). introduction of r2r3-myb transcription factor rosea1, the master regulator anthocyanin biosynthes ...201324205014
generation and analysis of a large-scale expressed sequence tag database from a full-length enriched cdna library of developing leaves of gossypium hirsutum l.cotton (gossypium hirsutum l.) is one of the world's most economically-important crops. however, its entire genome has not been sequenced, and limited resources are available in genbank for understanding the molecular mechanisms underlying leaf development and senescence.201324146870
large-scale resource development in gossypium hirsutum l. by 454 sequencing of genic-enriched libraries from six diverse genotypes.the sequence information has been proved to be an essential genomic resource in case of crop plants for their genetic improvement and better utilization by humans. to dissect the gossypium hirsutum genome for large-scale development of genomic resources, we adopted hypomethylated restriction-based genomic enrichment strategy to sequence six diverse genotypes. approximately 5.2-gb data (more than 18.36 million reads) was generated which, after assembly, represents nearly 1.27-gb genomic sequences ...201323782852
transcript profiling by microarray and marker analysis of the short cotton (gossypium hirsutum l.) fiber mutant ligon lintless-1 (li1).cotton fiber length is very important to the quality of textiles. understanding the genetics and physiology of cotton fiber elongation can provide valuable tools to the cotton industry by targeting genes or other molecules responsible for fiber elongation. ligon lintless-1 (li1) is a monogenic mutant in upland cotton (gossypium hirsutum) which exhibits an early cessation of fiber elongation resulting in very short fibers (< 6 mm) at maturity. this presents an excellent model system for studying ...201323767687
molecular cloning and function analysis of two squamosa-like mads-box genes from gossypium hirsutum l.the mads-box genes encode a large family of transcription factors having diverse roles in plant development. the squamosa (squa)/apetala1 (ap1)/fruitfull (ful) subfamily genes are essential regulators of floral transition and floral organ identity. here we cloned two mads-box genes, ghmads22 and ghmads23, belonging to the squa/ap1/ful subgroup from gossypium hirsutum l. phylogenetic analysis and sequence alignment showed that ghmads22 and ghmads23 belonged to the euful and euap1 subclades, respe ...201323718551
transcriptome profiling reveals auxin and cytokinin regulating somatic embryogenesis in different sister lines of cotton cultivar ccri24.to get a broader view on the molecular mechanisms underlying somatic embryogenesis (se) in cotton (gossypium hirsutum l.), global analysis of cotton transcriptome dynamics during se in different sister lines was performed using rna-seq. a total of 204 349 unigenes were detected by de novo assembly of the 214 977 462 illumina reads. the quantitative reverse transcription-polymerase chain reaction (qrt-pcr) measurements were positively correlated with the rna-seq results for almost all the tested ...201323710882
resolving multiple host use of an emergent pest of cotton with microsatellite data and chloroplast markers (creontiades dilutus stål; hemiptera, miridae).following the global uptake of transgenic cotton several hemipteran pests have emerged as primary targets for pesticide control. previous research on one such emergent pest: the green mirid, creontiades dilutus, indicated differential use of two crop hosts, cotton (gossypium hirsutum, malvaceae) and lucerne (alfalfa) (medicago sativa, fabaceae). we tested the hypothesis that this apparent demographic independence of lucerne and cotton inhabiting mirids is the result of cryptic species being asso ...201323702301
identification and characterization of (e)-β-caryophyllene synthase and α/β-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton.cotton (gossypium hirsutum l.) plants damaged by insects emit a blend of volatiles, including monoterpenes and sesquiterpenes, which can directly repel herbivores and/or indirectly protect the plant by attracting natural enemies of the herbivores. to understand the molecular basis of terpene biosynthesis and regulation in cotton, two terpene synthase genes, ghtps1 and ghtps2, were heterologously expressed and characterized. recombinant ghtps1 accepted farnesyl pyrophosphate as substrate and prod ...201324184450
linkage mapping and expression analysis of mirnas and their target genes during fiber development in cotton.micrornas (mirnas) are small, endogenously expressed, non-coding rna molecules involved in gene transcription and expression that combine with specific mrna site of target genes to inhibit protein synthesis or degrade mrna. since the first plant mirna was reported in 2002, numerous new mirnas and their targets have been discovered via high-throughput sequencing and computational approaches. however, the genetic variation of mirna genes is poorly understood due to the lack of mirna-specific dna m ...201324131852
genome-wide functional analysis of cotton (gossypium hirsutum) in response to drought.cotton is one of the most important crops for its natural textile fibers in the world. however, it often suffered from drought stress during its growth and development, resulting in a drastic reduction in cotton productivity. therefore, study on molecular mechanism of cotton drought-tolerance is very important for increasing cotton production. to investigate molecular mechanism of cotton drought-resistance, we employed rna-seq technology to identify differentially expressed genes in the leaves o ...201324260499
isolation and characterization of terpene synthases in cotton (gossypium hirsutum).cotton plants accumulate gossypol and related sesquiterpene aldehydes, which function as phytoalexins against pathogens and feeding deterrents to herbivorous insects. however, to date little is known about the biosynthesis of volatile terpenes in this crop. herein is reported that 5 monoterpenes and 11 sesquiterpenes from extracts of a glanded cotton cultivar, gossypium hirsutum cv. ccri12, were detected by gas chromatography-mass spectrometry (gc-ms). by est data mining combined with rapid ampl ...201324074555
genome-wide analysis of small rnas reveals eight fiber elongation-related and 257 novel micrornas in elongating cotton fiber cells.micrornas (mirnas) and other types of small regulatory rnas play critical roles in the regulation of gene expression at the post-transcriptional level in plants. cotton is one of the most economically important crops, but little is known about the roles of mirnas during cotton fiber elongation.201324044642
response of mirnas and their targets to salt and drought stresses in cotton (gossypium hirsutum l.).micrornas (mirnas) are an important gene regulator, controlling almost all biological and metabolic processes, in both plants and animals. in this study, we investigated the effect of drought and salinity stress on the expression of mirnas and their targets in cotton (gossypium hirsutum l.). our results show that the expression change of mirnas and their targets were dose-dependent and tissue-dependent under salinity and drought conditions. the expression of mirnas in leaf was down-regulated und ...201323948080
the complete mitochondrial genome of gossypium hirsutum and evolutionary analysis of higher plant mitochondrial genomes.mitochondria are the main manufacturers of cellular atp in eukaryotes. the plant mitochondrial genome contains large number of foreign dna and repeated sequences undergone frequently intramolecular recombination. upland cotton (gossypium hirsutum l.) is one of the main natural fiber crops and also an important oil-producing plant in the world. sequencing of the cotton mitochondrial (mt) genome could be helpful for the evolution research of plant mt genomes.201323940520
evaluation and selection of reliable reference genes for gene expression under abiotic stress in cotton (gossypium hirsutum l.).reference genes are critical for normalization of the gene expression level of target genes. the widely used housekeeping genes may change their expression levels at different tissue under different treatment or stress conditions. therefore, systematical evaluation on the housekeeping genes is required for gene expression analysis. up to date, no work was performed to evaluate the housekeeping genes in cotton under stress treatment. in this study, we chose 10 housekeeping genes to systematically ...201323933278
genome-wide analysis of small rna and novel microrna discovery during fiber and seed initial development in gossypium hirsutum. l.cotton is the source of the most important, renewable natural textile fiber and oil in the world. micrornas (mirnas) are endogenous, non-coding, approximately 18-24 nucleotides long rnas and function in the negative regulation of their target genes. two mostly overlapping libraries of small rna molecules were constructed and sequenced, and served as repetition sets of data to identify mirnas involved in fiber initiation and seed development. the d genome sequence of gossypium raimondii was used ...201323922789
molecular characterisation of two homoeologous elicitor-responsive lipin genes in cotton.the identification and molecular characterisation of two lipin-like gene copies (ghlipn) in cotton, gossypium hirsutum, an allotetraploid derived from two progenitor diploid gossypium species, is described. sequence analyses of the ghlipn copies, designated ghlipn-1 and -2, revealed that they contain 11 exons, separated by ten introns. they each have a 2,643 bp open reading frame that encodes 880 aa proteins, and share a 97.7 and 95.5 % sequence similarity at the translated nucleotide and amino ...201323897433
bac-pool sequencing and analysis of large segments of a12 and d12 homoeologous chromosomes in upland cotton.although new and emerging next-generation sequencing (ngs) technologies have reduced sequencing costs significantly, much work remains to implement them for de novo sequencing of complex and highly repetitive genomes such as the tetraploid genome of upland cotton (gossypium hirsutum l.). herein we report the results from implementing a novel, hybrid sanger/454-based bac-pool sequencing strategy using minimum tiling path (mtp) bacs from ctg-3301 and ctg-465, two large genomic segments in a12 and ...201324116150
cotton bracts are adapted to a microenvironment of concentrated co2 produced by rapid fruit respiration.elucidation of the mechanisms by which plants adapt to elevated co2 is needed; however, most studies of the mechanisms investigated the response of plants adapted to current atmospheric co2. the rapid respiration rate of cotton (gossypium hirsutum) fruits (bolls) produces a concentrated co2 microenvironment around the bolls and bracts. it has been observed that the intercellular co2 concentration of a whole fruit (bract and boll) ranges from 500 to 1300 µmol mol(-1) depending on the irradiance, ...201323625144
comparative expression profiling of mirna during anther development in genetic male sterile and wild type cotton.genetic male sterility (gms) in cotton (gossypium hirsutum) plays an important role in the utilization of hybrid vigor. however, the molecular mechanism of the gms is still unclear. while numerous studies have demonstrated that micrornas (mirna) regulate flower and anther development, whether different small rna regulations exist in gms and its wild type is unclear. a deep sequencing approach was used to investigate the global expression and complexity of small rnas during cotton anther developm ...201323597285
variations and transmission of qtl alleles for yield and fiber qualities in upland cotton cultivars developed in china.cotton is the world's leading cash crop, and genetic improvement of fiber yield and quality is the primary objective of cotton breeding program. in this study, we used various approaches to identify qtls related to fiber yield and quality. firstly, we constructed a four-way cross (4wc) mapping population with four base core cultivars, stoneville 2b, foster 6, deltapine 15 and zhongmiansuo no.7 (cri 7), as parents in chinese cotton breeding history and identified 83 qtls for 11 agronomic and fibe ...201323468939
cotton ghbak1 mediates verticillium wilt resistance and cell death.virus-induced gene silencing (vigs) offers a powerful approach for functional analysis of individual genes by knocking down their expression. we have adopted this approach to dissect gene functions in cotton resistant to verticillium wilt, one of the most devastating diseases worldwide. we showed here that highly efficient vigs was obtained in a cotton breeding line (ca4002) with partial resistance to verticillium wilt, and ghmkk2 and ghve1 are required for its resistance to verticillium wilt. a ...201323675706
polycat: a resource for genome categorization of sequencing reads from allopolyploid organisms.read mapping is a fundamental part of next-generation genomic research but is complicated by genome duplication in many plants. categorizing dna sequence reads into their respective genomes enables current methods to analyze polyploid genomes as if they were diploid. we present polycat-a pipeline for mapping and categorizing all types of next-generation sequence data produced from allopolyploid organisms. polycat uses gsnap's single-nucleotide polymorphism (snp)-tolerant mapping to minimize the ...201323450226
neonicotinoid insecticides alter induced defenses and increase susceptibility to spider mites in distantly related crop plants.chemical suppression of arthropod herbivores is the most common approach to plant protection. insecticides, however, can cause unintended, adverse consequences for non-target organisms. previous studies focused on the effects of pesticides on target and non-target pests, predatory arthropods, and concomitant ecological disruptions. little research, however, has focused on the direct effects of insecticides on plants. here we demonstrate that applications of neonicotinoid insecticides, one of the ...201323658754
using genome-referenced expressed sequence tag assembly to analyze the origin and expression patterns of gossypium hirsutum transcripts.we assembled a total of 297,239 gossypium hirsutum (gh, a tetraploid cotton, aadd) expressed sequence tag (est) sequences that were available in the national center for biotechnology information database, with reference to the recently published g. raimondii (gr, a diploid cotton, dd) genome, and obtained 49,125 unigenes. the average lengths of the unigenes were increased from 804 and 791 bp in two previous est assemblies to 1,019 bp in the current analysis. the number of putative cotton unigene ...201323675784
cotton anngh3 encoding an annexin protein is preferentially expressed in fibers and promotes initiation and elongation of leaf trichomes in transgenic arabidopsis.the annexins are a multifamily of calcium-regulated phospholipid-binding proteins. to investigate the roles of annexins in fiber development, four genes encoding putative annexin proteins were isolated from cotton (gossypium hirsutum) and designated anngh3, anngh4, anngh5, and anngh6. quantitative reverse transcription-polymerase chain reaction (qrt-pcr) results indicated that anngh3, anngh4, and anngh5 were preferentially expressed in fibers, while the transcripts of anngh6 were predominantly a ...201323651035
cotton gasl genes encoding putative gibberellin-regulated proteins are involved in response to ga signaling in fiber development.gast (ga-stimulated transcript)-like genes have been reported as targets of ga regulation in some plant species. in this study, we isolated seven gast-like cdnas from cotton (gossypium hirsutum) cdna libraries (designated as ghgasl1-ghgasl7). meanwhile, the genomic dna clones corresponding to the seven ghgasl genes were isolated by using pcr amplification technique. analysis of gene structure revealed that four genes (ghgasl1/3/5/6) contain two exons and one intron, while the rest have four exon ...201323645033
chloroplast-targeted expression of recombinant crystal-protein gene in cotton: an unconventional combat with resistant pests.plants transformed with single bt gene are liable to develop insect resistance and this has already been reported in a number of studies carried out around the world where bt cotton was cultivated on commercial scale. later, it was envisaged to transform plants with more than one bt genes in order to combat with resistant larvae. this approach seems valid as various bt genes possess different binding domains which could delay the likely hazards of insect resistance against a particular bt toxin. ...201323643479
a fasciclin-like arabinogalactan protein, ghfla1, is involved in fiber initiation and elongation of cotton.arabinogalactan proteins (agps) are involved in many aspects of plant development. in this study, biochemical and genetic approaches demonstrated that agps are abundant in developing fibers and may be involved in fiber initiation and elongation. to further investigate the role of agps during fiber development, a fasciclin-like arabinogalactan protein gene (ghfla1) was identified in cotton (gossypium hirsutum). overexpression of ghfla1 in cotton promoted fiber elongation, leading to an increase i ...201323349362
agrobacterium-mediated transformation of cotton (gossypium hirsutum) shoot apex with a fungal phytase gene improves phosphorus acquisition.cotton is an important world economic crop plant. it is considered that cotton is recalcitrant to in vitro proliferation. somatic embryogenesis and plant regeneration has been successful by using hypocotyl, whereas it is highly genotype dependent. here, a genotype-independent cotton regeneration protocol from shoot apices is presented. shoot apices from 3- to 5-day-old seedlings of cotton are infected with an agrobacterium strain, eha105, carrying the binary vector pc-ksa contained phytase gene ...201323143496
two modified rna extraction methods compatible with transcript profiling and gene expression analysis for cotton roots.efficient isolation of high-quality rna is of prime importance for optimal transcript profiling results and further gene expression analysis. however, it is difficult for cotton roots because of lower-than-average rna content and high content of polysaccharides, polyphenols, and other secondary metabolites. to develop simple and reliable protocols for high-quality rna extraction from cotton roots for transcript profiling and gene expression analysis, some modifications were introduced to a repor ...201323581784
mapping heterotic loci for yield and agronomic traits using chromosome segment introgression lines in cotton.in the present study, a set of chromosome segment introgression lines (csils) using gossypium hirsutum l. tm-1 as the recipient parent and g. barbadense hai7124 as the donor parent were used to explore the genetic basis of heterosis for interspecific hybrids. two sets of f₁ populations individually derived from csils crossing with both parents were configured to investigate heterotic loci (hl) and substitution effect loci (sl). a total of 58 hl and 39 sl were identified in 3 years. one stable hl ...201323570369
analysis of flavonoids and the flavonoid structural genes in brown fiber of upland cotton.as a result of changing consumer preferences, cotton (gossypium hirsutum l.) from varieties with naturally colored fibers is becoming increasingly sought after in the textile industry. the molecular mechanisms leading to colored fiber development are still largely unknown, although it is expected that the color is derived from flavanoids.201323527031
genotypic variability for root/shoot parameters under water stress in some advanced lines of cotton (gossypium hirsutum l.).research pertaining to genetic variability parameters, heritability, and genotypic, phenotypic, simple, and environmental correlations for various seedling traits in five elite advanced cotton (gossypium hirsutum l.) lines (fh-113, fh-114, fh-941, fh-942, and fh-2015) and one check (cim-496) was carried out during october and november 2010 under greenhouse conditions at the cotton research institute (faisalabad, pakistan). material was raised in plastic tubes with a randomized complete block des ...201323512672
integrated metabolomics and genomics analysis provides new insights into the fiber elongation process in ligon lintless-2 mutant cotton (gossypium hirsutum l.).the length of cotton fiber is an important agronomic trait characteristic that directly affects the quality of yarn and fabric. the cotton (gossypium hirsutum l.) fiber mutation, ligon lintless-2, is controlled by a single dominant gene (li(2)) and results in extremely shortened lint fibers on mature seeds with no visible pleiotropic effects on vegetative growth and development. the li(2) mutant phenotype provides an ideal model system to study fiber elongation. to understand metabolic processes ...201323497242
field-acclimated gossypium hirsutum cultivars exhibit genotypic and seasonal differences in photosystem ii thermostability.previous investigations have demonstrated that photosystem ii (psii) thermostability acclimates to prior exposure to heat and drought, but contrasting results have been reported for cotton (gossypium hirsutum). we hypothesized that psii thermotolerance in g. hirsutum would acclimate to environmental conditions during the growing season and that there would be differences in psii thermotolerance between commercially-available u.s. cultivars. to this end, three cotton cultivars were grown under dr ...201323246028
preference of a polyphagous mirid bug, apolygus lucorum (meyer-dür) for flowering host plants.apolygus lucorum (meyer-dür) (hemiptera: miridae) is one of the most important herbivores in a broad range of cultivated plants, including cotton, cereals, vegetables, and fruit crops in china. in this manuscript, we report on a 6-year long study in which (adult) a. lucorum abundance was recorded on 174 plant species from 39 families from early july to mid-september. through the study period per year, the proportion of flowering plants exploited by adult a. lucorum was significantly greater than ...201323874835
analyzing blends of herbivore-induced volatile organic compounds with factor analysis: revisiting "cotton plant, gossypium hirsutum l., defense in response to nitrogen fertilization".many herbivorous, predaceous, and parasitic insects use constitutive and herbivore-induced volatile organic compounds (vocs) to locate their respective host plant, prey, and hosts. multivariate statistical tools (e.g., factor analysis) are recognized increasingly as an appropriate approach for analyzing intercorrelated data such as presence/absence or quantities of vocs. one challenge of implementing factor analysis is determining how many new variables (factors) to retain in the final analysis. ...201323786100
synthesis of resveratrol glycosides by cultured plant cells.incubation of cultured cells of glycine max with trans-resveratrol gave its 3-o-beta-d- and 4'-o-beta-d-glucosides. cultured gossypium hirsutum cells glycosylated trans-resveratrol to its 3-o-beta-d-, 4'-o-beta-d-, and 3,4'-o-beta-d-diglucosides. on the other hand, trans-resveratrol was converted into cis-resveratrol 4'-o-beta-d-glucoside, together with trans-resveratrol 3-o-beta-d-glucoside and trans-resveratrol 4'-o-beta-d-glucoside, by eucalyptus perriniana.201323980421
cysteine protease enhances plant-mediated bollworm rna interference.oral ingestion of plant-expressed double stranded rna (dsrna) triggers target gene suppression in insect. an important step of this process is the transmission of dsrna from plant to midgut cells. insect peritrophic matrix (pm) presents a barrier that prevents large molecules from entering midgut cells. here, we show that uptake of plant cysteine proteases, such as ghcp1 from cotton (gossypium hirsutum) and atcp2 from arabidopsis, by cotton bollworm (helicoverpa armigera) larvae resulted in atte ...201323460027
likelihood of stink bugs colonizing crops: a case study in southeastern farmscapes.stink bugs, including nezara viridula (l.), euschistus servus (say), and chinavia hilaris (say), are economic pests across agricultural farmscapes where they can colonize closely associated crops. this 4-yr on-farm study was conducted to examine the likelihood of these three stink bug species colonizing crops in corn-cotton, corn-peanut-cotton, and peanut-cotton farmscapes by using odds ratios. corn (zea mays l.), peanut (arachis hypogaea l.), and cotton (gossypium hirsutum l.) served as host pl ...201323726052
mapping quantitative trait loci for lint yield and fiber quality across environments in a gossypium hirsutum × gossypium barbadense backcross inbred line population.identification of stable quantitative trait loci (qtls) across different environments and mapping populations is a prerequisite for marker-assisted selection (mas) for cotton yield and fiber quality. to construct a genetic linkage map and to identify qtls for fiber quality and yield traits, a backcross inbred line (bil) population of 146 lines was developed from a cross between upland cotton (gossypium hirsutum) and egyptian cotton (gossypium barbadense) through two generations of backcrossing u ...201323064252
interspecific chromosomal effects on agronomic traits in gossypium hirsutum by ad analysis using intermated g. barbadense chromosome substitution lines.the untapped potential of the beneficial alleles from gossypium barbadense l. has not been well utilized in g. hirsutum l. (often referred to as upland cotton) breeding programs. this is primarily due to genomic incompatibility and technical challenges associated with conventional methods of interspecific introgression. in this study, we used a hypoaneuploid-based chromosome substitution line as a means for systematically introgressing g. barbadense doubled-haploid line '3-79' germplasm into a c ...201322945267
modulation of reproductive behaviors by non-host volatiles in the polyphagous egyptian cotton leafworm, spodoptera littoralis.in order to locate mates, food, and oviposition sites, insects mainly rely on volatile cues released by their sexual partners, food sources, and host and non-host plants. calling, mating, and oviposition behaviors, as well as fecundity and longevity, of newly emerged spodoptera littoralis (bois.) moths were recorded in the presence of volatiles from leaves of a host plant, gossypium hirsutum (cotton) and two non-host plants, adhatoda vasica (av) or picea abies (spruce), either alone or in host/n ...201324105603
functional analysis of the seed coat-specific gene gbmyb2 from cotton.myb transcription factors are essential for cotton fiber development. we isolated the r2r3-myb gene gbmyb2 from gossypium barbadense. rna in situ hybridization analysis showed that gbmyb2 is mainly expressed in the outer integuments of cotton ovules and in elongating fibers. gbmyb2 expression increased throughout fiber initiation and during the elongation stage. the expression level of gbmyb2 was higher in the gossypium hirsutum cultivar xu142 than in the xu142 (fl) mutant. overexpression of gbm ...201324036393
specific response to herbivore-induced de novo synthesized plant volatiles provides reliable information for host plant selection in a moth.animals depend on reliable sensory information for accurate behavioural decisions. for herbivorous insects it is crucial to find host plants for feeding and reproduction, and these insects must be able to differentiate suitable from unsuitable plants. volatiles are important cues for insect herbivores to assess host plant quality. it has previously been shown that female moths of the egyptian cotton leafworm, spodoptera littoralis (lepidoptera: noctuidae), avoid oviposition on damaged cotton gos ...201323737555
a genetic and metabolic analysis revealed that cotton fiber cell development was retarded by flavonoid naringenin.the cotton (gossypium spp.) fiber is a unique elongated cell that is useful for investigating cell differentiation. previous studies have demonstrated the importance of factors such as sugar metabolism, the cytoskeleton, and hormones, which are commonly known to be involved in plant cell development, while the secondary metabolites have been less regarded. by mining public data and comparing analyses of fiber from two cotton species (gossypium hirsutum and gossypium barbadense), we found that th ...201323535943
an integrative analysis of four cesa isoforms specific for fiber cellulose production between gossypium hirsutum and gossypium barbadense.cotton fiber is an excellent model system of cellulose biosynthesis; however, it has not been widely studied due to the lack of information about the cellulose synthase (cesa) family of genes in cotton. in this study, we initially identified six full-length cesa genes designated as ghcesa5-ghcesa10. phylogenetic analysis and gene co-expression profiling revealed that cesa1, cesa2, cesa7, and cesa8 were the major isoforms for secondary cell wall biosynthesis, whereas cesa3, cesa5, cesa6, cesa9, a ...201323508664
cotton fiber cell walls of gossypium hirsutum and gossypium barbadense have differences related to loosely-bound xyloglucan.cotton fiber is an important natural textile fiber due to its exceptional length and thickness. these properties arise largely through primary and secondary cell wall synthesis. the cotton fiber of commerce is a cellulosic secondary wall surrounded by a thin cuticulated primary wall, but there were only sparse details available about the polysaccharides in the fiber cell wall of any cotton species. in addition, gossypium hirsutum (gh) fiber was known to have an adhesive cotton fiber middle lamel ...201323457548
insights into the evolution of cotton diploids and polyploids from whole-genome re-sequencing.understanding the composition, evolution, and function of the gossypium hirsutum (cotton) genome is complicated by the joint presence of two genomes in its nucleus (at and dt genomes). these two genomes were derived from progenitor a-genome and d-genome diploids involved in ancestral allopolyploidization. to better understand the allopolyploid genome, we re-sequenced the genomes of extant diploid relatives that contain the a1 (gossypium herbaceum), a2 (gossypium arboreum), or d5 (gossypium raimo ...201323979935
fate and toxicity of endosulfan in namoi river water and bottom sediment.endosulfan (6,7,8,9,10,10,-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepine-3-oxide) sorption (standardized to 1% total organic carbon and dry weight) was significantly (p < 0.05) more concentrated on the large (>63 microm) particle fraction compared with smaller size fractions (<5 microm and 5-24 microm) of bottom sediments from the namoi river, australia. following completion of the particle size fractionation (6 to 12 wk) and a sediment toxicity assessment (2 wk), the s ...201311401264
endosulfan transport: i. integrative assessment of airborne and waterborne pathways.to reduce endosulfan (c9h6o3cl6s; 6,7,8,9,10,10-hexachloro-1,5, 5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepin 3-oxide) contamination in rivers and waterways, it is important to know the relative significances of airborne transport pathways (including spray drift, vapor transport, and dust transport) and waterborne transport pathways (including overland and stream runoff). this work uses an integrated modeling approach to assess the absolute and relative contributions of these pathways ...201311401261
narrow-waveband reflectance ratios for remote estimation of nitrogen status in cotton.tailoring nitrogen (n) fertilizer applications to cotton (gossypium hirsutum l.) in response to leaf n status may optimize n use efficiency and reduce off-site effects of excessive fertilizer use. this study compared leaf and canopy reflectance within the 350 to 950 nm range in order to identify reflectance ratios sensitive to leaf chlorophyll (chl), and hence n status, in cotton. plants were grown outdoors in large pots using half-strength hoagland's (control) solution until some three-row plot ...201312371160
simulating endosulfan transport in runoff from cotton fields in australia with the gleams model.endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9methano-2,4,3-benzodioxathiepin 3-oxide), a pesticide that is highly toxic to aquatic organisms, is widely used in the cotton (gossypium hirsutum l.) industry in australia and is a risk to the downstream riverine environment. we used the gleams model to evaluate the effectiveness of a range of management scenarios aimed at minimizing endosulfan transport in runoff at the field scale. the field management scenarios simulated were (i) ...201311401260
spray drift of pesticides arising from aerial application in cotton.this paper presents results from field studies carried out during the 1993-1998 australian cotton (gossypium hirsutum l.) seasons to monitor off-target droplet movement of endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepin 3-oxide) insecticide applied to a commercial cotton crop. averaged over a wide range of conditions, off-target deposition 500 m downwind of the field boundary was approximately 2% of the field-applied rate with oil-based applicatio ...201311401259
off-site movement of endosulfan from irrigated cotton in new south wales.the fate and transport of endosulfan (6,7,8,9,10,10-hexachloro-1,5, 5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepin 3-oxide) applied to cotton (gossypium hirsutum l.) fields were studied throughout three consecutive years on two selected locations in new south wales (australia). rates of dissipation from foliage and soil, volatilization from the field, and transport of residues in irrigation and/or storm runoff waters were measured in order to estimate a total field balance. dissipation ...201311401258
fly ash as a liming material for cotton.a field experiment was conducted to determine the effect of fly ash from a coal combustion electric power facility on soil acidity in a cotton (gossypium hirsutum l.) field. fresh fly ash was applied to a bosket fine sandy loam (fine-loamy, mixed, thermic mollic hapludalf) soil with an initial soil ph(salt) of 4.8. the fly ash was equivalent to 42 g kg(-1) calcium carbonate with 97% passing through a 60 mesh (u.s. standard) sieve. fly ash was applied one day before cotton planting in 1999 at 0, ...201314964389
carbon dioxide efflux from soil with poultry litter applications in conventional and conservation tillage systems in northern alabama.increased co2 release from soils resulting from agricultural practices such as tillage has generated concerns about contributions to global warming. maintaining current levels of soil c and/or sequestering additional c in soils are important mechanisms to reduce co2 in the atmosphere through production agriculture. we conducted a study in northern alabama from 2003 to 2006 to measure co2 efflux and c storage in long-term tilled and non-tilled cotton (gossypium hirsutum l.) plots receiving poultr ...201318396538
nitrogen and phosphorus runoff losses from variable and constant intensity rainfall simulations on loamy sand under conventional and strip tillage systems.further studies on the quality of runoff from tillage and cropping systems in the southeastern usa are needed to refine current risk assessment tools for nutrient contamination. our objective was to quantify and compare effects of constant (ic) and variable (iv) rainfall intensity patterns on inorganic nitrogen (n) and phosphorus (p) losses from a tifton loamy sand (plinthic kandiudult) cropped to cotton (gossypium hirsutum l.) and managed under conventional (ct) or strip-till (st) systems. we s ...201317485716
in vitro culture of callus tissues and cell suspensions from okra (hibiscus esculentus l.) and cotton (gossypium hirsutum l.). 20134842933
characterization of the global transcriptome for cotton (gossypium hirsutum l.) anther and development of ssr marker.cotton is an important fiber plant, and it's attractive to elucidate the molecular mechanism of anther development due to the close relationship between the anther fertility and boll-setting, and also fiber yield. in the present paper, 47.2 million paired-end reads with average length of 82.87 bp from the anthers of tm-1 (gossypium hirsutum l.), a genetic standard line, were generated through transcriptome sequencing, and 210,965 unigenes of more than 100 bp were obtained. blast, kegg, cog, and ...201425178523
direct visualization of horizontal gene transfer in cotton plants.plant mitochondrial and chloroplast genes that underwent horizontal transfer have been identified by parasite and grafting systems, respectively. here, we directly observed 3 horizontal gene transfer (hgt) events in the 45 second axillary shoots of grafted cotton plants (gossypium barbadense and gossypium hirsutum) after extirpating the first axillary bud. the second axillary shoots showed phenotypic variations in cotton flowers and seeds that were evidence of spontaneous development from cells ...201425160847
identification of centromeric regions on the linkage map of cotton using centromere-related repeats.centromere usually contains high-copy-number retrotransposons and satellite repeats, which are difficult to map, clone and sequence. currently, very little is known about the centromere in cotton. here, we sequenced a bacterial artificial chromosome (bac) mapping to the centromeric region and predicted four long-terminal-repeat (ltr) retrotransposons. they were located in the heterochromatic centromeric regions of all 52 pachytene chromosomes in gossypium hirsutum. fiber-fish mapping revealed th ...201425238895
in silico prediction of cotton (gossypium hirsutum) encoded micrornas targets in the genome of cotton leaf curl allahabad virus.cotton leaf curl allahabad virus (clcuav) belongs to genus begomovirus, family geminiviridae. it has single stranded monopartite dna genome transmitted by whitefly (bemisia tabaci). micrornas (mirnas) belong to class of endogeneous small rnas which suppress expression of genes following cleavage or translational inhibition of target messenger rnas. they are demonstrated to be involved in a number of plant processes such as, development, biotic and abiotic stresses. employing in silico approach, ...201424966530
performance of the striped mealybug ferrisia virgata cockerell (hemiptera: pseudococcidae) under variable conditions of temperature and mating.mealybugs have strong associations with their host plants due to their limitations for dispersal. thus, environmental conditions and host quality may impact the biological traits of mealybugs. to the best of our knowledge, we are the first to report on the biology of a brazilian population of the striped mealybug ferrisia virgata cockerell (hemiptera: pseudococcidae), which has recently been reported to infest cotton in brazil. we evaluated the development and reproductive performance of f. virg ...201427193398
ethnobotanical study of kani tribes in thoduhills of kerala, south india.india is having a rich vegetation with a wide variety of plants, because of the extreme variations in geographical and climatic conditions prevailing in the country. plants have been used since ancient times for the treatment of various ailments. especially, kani tribal communities in thodu hills of kerala meet their healthcare needs by using non-timber minor forest products and preparations based on traditional knowledge. they still depend on medicinal plants and most of them have a basic knowl ...201424393788
carcass merit and meat quality in suffolk lambs, katahdin lambs, and meat-goat kids finished on a grass-legume pasture with and without supplementation.the experiment evaluated traditional u.s. sheep (suffolk), hair sheep (katahdin), and meat goat (boer crossbred; goat) carcass and meat quality parameters when finished on pasture with and without supplemental whole cottonseed (gossypium hirsutum l.). supplemented animals had greater ribeye area (p<0.01), body wall thickness (p<0.001), and lean quality score (p<0.05) than unsupplemented animals. whole cottonseed increased fatty acids (fa) 18:1 trans-10, 18:1 trans-12, 18:2, and omega6:omega3 rat ...201424971809
myb80 homologues in arabidopsis, cotton and brassica: regulation and functional conservation in tapetal and pollen development.the arabidopsis atmyb80 transcription factor regulates genes involved in pollen development and controls the timing of tapetal programmed cell death (pcd). downregulation of atmyb80 expression precedes tapetal degradation. inhibition of atmyb80 expression results in complete male sterility. full-length atmyb80 homologs have been isolated in wheat, rice, barley and canola (c genome).201425311582
ssr marker-assisted improvement of fiber qualities in gossypium hirsutum using g. barbadense introgression lines.this study demonstrates the first practical use of csils for the transfer of fiber quality qtls into upland cotton cultivars using ssr markers without detrimentally affecting desirable agronomic characteristics. gossypium hirsutum is characterized by its high lint production and medium fiber quality compared to extra-long staple cotton g. barbadense. transferring valuable traits or genes from g. barbadense into g. hirsutum is a promising but challenging approach through a traditional interspecif ...201424306319
reflectance and cyclic electron flow as an indicator of drought stress in cotton (gossypium hirsutum).the response and the functioning of the photosynthetic machinery of cotton, gossypium hirsutum during water stress was studied by leaf optical properties, linear (etrii) and cyclic electron flow (cef) and chlorophyll a fluorescence. we observed that in g. hirsutum, during water limitation, chlorophyll b showed the best correlation with reflectance at 731 nm and is a better indicator of drought. fv /fm was observed to be very insensitive to mild water stress. however, during severe water stress t ...201424246098
genetic diversity and population structure in the us upland cotton (gossypium hirsutum l.).genetic diversity and population structure in the us upland cotton was established and core sets of allelic richness were identified for developing association mapping populations in cotton. elite plant breeding programs could likely benefit from the unexploited standing genetic variation of obsolete cultivars without the yield drag typically associated with wild accessions. a set of 381 accessions comprising 378 upland (gossypium hirsutum l.) and 3 g. barbadense l. accessions of the united stat ...201424170350
mapping and genomic targeting of the major leaf shape gene (l) in upland cotton (gossypium hirsutum l.).a major leaf shape locus (l) was mapped with molecular markers and genomically targeted to a small region in the d-genome of cotton. by using expression analysis and candidate gene mapping, two lmi1 -like genes are identified as possible candidates for leaf shape trait in cotton. leaf shape in cotton is an important trait that influences yield, flowering rates, disease resistance, lint trash, and the efficacy of foliar chemical application. the leaves of okra leaf cotton display a significantly ...201424158249
cellulose synthases: new insights from crystallography and modeling.detailed information about the structure and biochemical mechanisms of cellulose synthase (cels) proteins remained elusive until a complex containing the catalytic subunit (bcsa) of cels from rhodobacter sphaeroides was crystalized. additionally, a 3d structure of most of the cytosolic domain of a plant cels (ghcesa1 from cotton, gossypium hirsutum) was produced by computational modeling. this predicted structure contributes to our understanding of how plant cels proteins may be similar and diff ...201424139443
changes in the cell wall and cellulose content of developing cotton fibers investigated by ftir spectroscopy.fourier transform infrared (ftir) spectra of cotton fibers harvested at different stages of development were acquired using universal attenuated total reflectance ftir (uatr-ftir). the main goal of the study was to monitor cell wall changes occurring during different phases of cotton fiber development. two cultivars of gossypium hirsutum l. were planted in a greenhouse (texas marker-1 and tx55). on the day of flowering, individual flowers were tagged and bolls were harvested. from fibers harvest ...201424188832
both methylerythritol phosphate and mevalonate pathways contribute to biosynthesis of each of the major isoprenoid classes in young cotton seedlings.in higher plants, both the methylerythritol phosphate (mep) and mevalonate (mva) pathways contribute to the biosynthesis of isoprenoids. however, despite a significant amount of research on the activity of these pathways under different conditions, the relative contribution of each to the biosynthesis of diverse isoprenoids remains unclear. in this work, we examined the formation of several classes of isoprenoids in cotton (gossypium hirsutum l.). after feeding [5,5-(2)h2]-1-deoxy-d-xylulose ([5 ...201424359633
comparative evolutionary and developmental dynamics of the cotton (gossypium hirsutum) fiber transcriptome.the single-celled cotton (gossypium hirsutum) fiber provides an excellent model to investigate how human selection affects phenotypic evolution. to gain insight into the evolutionary genomics of cotton domestication, we conducted comparative transcriptome profiling of developing cotton fibers using rna-seq. analysis of single-celled fiber transcriptomes from four wild and five domesticated accessions from two developmental time points revealed that at least one-third and likely one-half of the g ...201424391525
a mads-box gene is specifically expressed in fibers of cotton (gossypium hirsutum) and influences plant growth of transgenic arabidopsis in a ga-dependent manner.in this study, a cdna, ghmads14, encoding a typical mads-box protein with 223 amino acids was isolated from a cotton cdna library. fluorescent microscopy indicated that the ghmads14 protein was localized in the cell nucleus. ghmads14 was specifically expressed in the elongating fibers, and its expression was gradually enhanced at early stages of fiber elongation and reached its peak in 9-10 dpa fibers. overexpression of ghmads14 in arabidopsis hindered plant growth. measurement and statistical a ...201424374505
independent replication of mitochondrial genes supports the transcriptional program in developing fiber cells of cotton (gossypium hirsutum l.).the mitochondrial genomes of flowering plants exist both as a "master circle" chromosome and as numerous subgenomic sublimons that are generated by intramolecular recombination. differential stability or replication of these sublimons allows individual mitochondrial gene copy numbers to vary independently between different cell types and developmental stages. our objective was to determine the relationship between mitochondrial gene copy number and transcript abundance in the elongating fiber ce ...201424768176
waterlogging during flowering and boll forming stages affects sucrose metabolism in the leaves subtending the cotton boll and its relationship with boll weight.the work explored sucrose metabolism in the leaves subtending the cotton boll (sbl) and its role in boll weight after waterlogging in cotton. results showed that net photosynthesis rate (pn), relative water content, contents of chlorophyll a and chlorophyll b, initial ribulose-1,5-bisphosphate carboxylase-oxygenase (rubisco) activity and cytosolic fructose-1, 6-bisphosphatase (cy-fbpase) activity decreased with waterlogging in the sbl on fruiting branches 2-3 (fb2-3) and fb6-7. activities of suc ...201424767118
comparative transcriptome analysis of short fiber mutants ligon-lintless 1 and 2 reveals common mechanisms pertinent to fiber elongation in cotton (gossypium hirsutum l.).understanding the molecular processes affecting cotton (gossypium hirsutum) fiber development is important for developing tools aimed at improving fiber quality. short fiber cotton mutants ligon-lintless 1 (li1) and ligon-lintless 2 (li2) are naturally occurring, monogenic mutations residing on different chromosomes. both mutations cause early cessation in fiber elongation. these two mutants serve as excellent model systems to elucidate molecular mechanisms relevant to fiber length development. ...201424748059
gene expression profile analysis of ligon lintless-1 (li1) mutant reveals important genes and pathways in cotton leaf and fiber development.ligon lintless-1 (li1) is a monogenic dominant mutant of gossypium hirsutum (upland cotton) with a phenotype of impaired vegetative growth and short lint fibers. despite years of research involving genetic mapping and gene expression profile analysis of li1 mutant ovule tissues, the gene remains uncloned and the underlying pathway of cotton fiber elongation is still unclear. in this study, we report the whole genome-level deep-sequencing analysis of leaf tissues of the li1 mutant. differentially ...201424279997
the role of somatic embryogenesis receptor-like kinase 1 in controlling pollen production of the gossypium anther.in flowering plants, male gametophytes are generated in anthers from microsporocytes. however, more evidence is needed to reveal the genetic mechanisms which regulate the differentiation and interaction of these highly specialized cells in anthers. here we report the characterization of a series of male-sterile cotton (gossypium hirsutum) mutants, including mutants with normal fertility, semi-sterility and complete sterility. these mutants are forms of transgenic cotton containing rnai vectors w ...201424276918
modelling the structural response of cotton plants to mepiquat chloride and population density.cotton (gossypium hirsutum) has indeterminate growth. the growth regulator mepiquat chloride (mc) is used worldwide to restrict vegetative growth and promote boll formation and yield. the effects of mc are modulated by complex interactions with growing conditions (nutrients, weather) and plant population density, and as a result the effects on plant form are not fully understood and are difficult to predict. the use of mc is thus hard to optimize.201424489020
association analysis of fiber quality traits and exploration of elite alleles in upland cotton cultivars/accessions (gossypium hirsutum l.).exploring the elite alleles and germplasm accessions related to fiber quality traits will accelerate the breeding of cotton for fiber quality improvement. in this study, 99 gossypium hirsutum l. accessions with diverse origins were used to perform association analysis of fiber quality traits using 97 polymorphic microsatellite marker primer pairs. a total of 107 significant marker-trait associations were detected for three fiber quality traits under three different environments, with 70 detected ...201424428209
functional characterization of a bidirectional plant promoter from cotton leaf curl burewala virus using an agrobacterium-mediated transient assay.the c1 promoter expressing the ac1 gene, and v1 promoter expressing the av1 gene are located in opposite orientations in the large intergenic region of the cotton leaf curl burewala virus (clcubuv) genome. agro-infiltration was used to transiently express putative promoter constructs in nicotiana tabacum and gossypium hirsutum leaves, which was monitored by a gus reporter gene, and revealed that the bidirectional promoter of clcubuv transcriptionally regulates both the ac1 and av1 genes. the clc ...201424424501
molecular mapping and validation of a major qtl conferring resistance to a defoliating isolate of verticillium wilt in cotton (gossypium hirsutum l.).verticillium wilt (vw) caused by verticillium dahliae kleb is one of the most destructive diseases of cotton. development and use of a vw resistant variety is the most practical and effective way to manage this disease. identification of highly resistant genes/qtl and the underlining genetic architecture is a prerequisite for developing a vw resistant variety. a major qtl qvw-c6-1 conferring resistance to the defoliating isolate v991 was identified on chromosome 6 in lhb22×jm11 f2∶3 population i ...201424781706
related to aba-insensitive3(abi3)/viviparous1 and atabi5 transcription factor coexpression in cotton enhances drought stress adaptation.drought tolerance is an important trait being pursued by the agbiotech industry. abscisic acid (aba) is a stress hormone that mediates a multitude of processes in growth and development, water use efficiency (wue) and gene expression during seed development and in response to environmental stresses. arabidopsis b3-domain transcription factor related to aba-insensitive3 (abi3)/viviparous1 (namely atrav2) and basic leucine zipper (bzips) atabi5 or atabf3 transactivated aba-inducible promoter:gus r ...201424483851
the li2 mutation results in reduced subgenome expression bias in elongating fibers of allotetraploid cotton (gossypium hirsutum l.).next generation sequencing (rna-seq) technology was used to evaluate the effects of the ligon lintless-2 (li2) short fiber mutation on transcriptomes of both subgenomes of allotetraploid cotton (gossypium hirsutum l.) as compared to its near-isogenic wild type. sequencing was performed on 4 libraries from developing fibers of li2 mutant and wild type near-isogenic lines at the peak of elongation followed by mapping and polycat categorization of rna-seq data to the reference d5 genome (g. raimond ...201424598808
aberrant phenotype and transcriptome expression during fiber cell wall thickening caused by the mutation of the im gene in immature fiber (im) mutant in gossypium hirsutum l.the immature fiber (im) mutant of gossypium hirsutum l. is a special cotton fiber mutant with non-fluffy fibers. it has low dry weight and fineness of fibers due to developmental defects in fiber secondary cell wall (scw).201424483163
changes during leaf expansion of φpsii temperature optima in gossypium hirsutum are associated with the degree of fatty acid lipid saturation.in this project, we hypothesize that cotton (gossypium hirsutum) leaf temperature and the responses of leaf photosynthesis to temperature will change as the leaves expand and that differences between young and mature leaves will be associated with the proportion of saturated fatty acids in thylakoid and other membrane lipids. to that end, we studied main stem leaves obtained from plants growing in a temperature controlled greenhouse and at different times in the field season. we found that young ...201424594393
identification and functional analysis of a novel parvulin-type peptidyl-prolyl isomerase from gossypium hirsutum.plants have developed a variety of adaptive mechanisms to cope with stresses. a novel salt-induced gene was isolated during the screening of a nacl-induced cdna library of cotton seedlings. the gene was registered as accession number ay972810 in genbank. phylogenetic analysis suggested that the protein encoded by the gene belongs to the parvulin family of peptidyl-prolyl cis/trans isomerases (ppiases, ec 5.2.1.8). northern blot analysis indicated that the mrna accumulation of ghppi was induced b ...201424468661
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