Publications

foot pad enlargement as a measure of induced immunity to mycobacterium leprae.	foot pad enlargement (fpe) has been used as a measure of induced immunity to m. leprae, fpe peaked at 2-3 days, but it sometimes persisted for 4 weeks or more. both as the inducing and eliciting antigen, heat-killed m. leprae were effective, and the optimum dose was about 1 x 10(7) bacilli. higher doses were associated with flattening of the dose-response curve. disrupted bacilli were not effective in immunizing mice, but they elicited fpe responses in mice immunized with intact bacilli. cord fa ...	1980	7009447
evidence for species-specific lipid antigens in mycobacterium leprae.	a partially purified lipid fraction from mycobacterium leprae yielded distinct lines of precipitation with antisera from two lepromatus patients and from an infected armadillo. there was no reaction to the sera from patients with tuberculosis or a m. avium infection or to the serum from a normal armadillo. the activity in the lipid fraction was unaffected by mild alkali, and upon hydrolysis the fraction yielded 6-deoxyhexoses. this information suggests that the lipid antigens of m. leprae may be ...	1980	7009448
an improved embedding method for electron microscopy of lepromata.	spurr's resin mixture has been found to be the embedding material most suitable for electron microscopic studies of lepromata at present. like methacrylate, it can penetrate well into foamy structures and the leprosy bacilli within foamy structures. on the other hand, like epon 812, it is stable against the electron beam and can preserve ultrafine structures. additionally, we have found that the use of dimethylformamide or dimethylsulfoxide instead of propylene oxide as substituter improves the ...	1980	7009449
thoughts on the immunology of leprosy.		1980	7009450
identification problems of strain 0122.		1980	7009452
genetics and leprosy.		1980	7009982
multivariate analysis of the activity of rifamycins against rapidly growing mycobacteria, to define an in vitro screening model for their activity against mycobacterium leprae.	in an attempt to select a group of rapidly growing mycobacteria which could serve as a model for the in vitro screening of rifamycin derivatives for their activity against mycobacterium leprae, the mic values of 237 strains of rapidly growing mycobacteria were determined for 4 reference rifamycins with known activity against m. leprae and 19 rifamycins with unknown activity against m. leprae. study of the results by a multivariate statistical technique, the principal components analysis, defined ...	1980	7011330
hydrocarbons of petroleum: ideal substance for mycobacteria.		1980	6789600
the immunogenicity of killed mycobacteria.		1980	7012498
preliminary immunological studies in search of correlates of protective immunity carried out on some iranian leprosy patients and their families.		1980	7012499
dapsone-resistant leprosy in a population of bamako (mali).		1980	7012500
assaying dapsone in mouse diets.		1980	7012501
quantitative study of the separation of mycobacterium leprae from armadillo tissue.		1980	7012502
drug resistance in leprosy.		1980	7026426
leprosy without mycobacterium leprae.		1980	7021515
superoxide dismutase, peroxidatic activity and catalase in mycobacterium leprae purified from armadillo liver.	superoxide dismutase has been identified and peroxidatic activity demonstrated in mycobacterium leprae. the superoxide dismutase, shown indirectly to be a manganese-containing enzyme, was present at low activity in the cell-free extract. peroxidatic activity was detected in a haemoprotein on polyacrylamide gels, but quantitative assay was not possible. catalase, although present in a cell-free extract, appeared to be a host-derived enzyme, thus emphasizing the importance of establishing the auth ...	1980	7021767
[maintenance of armadillos in captivity and results of the inoculation of mycobacterium leprae].	the authors report their experiences in raising armadillos and the results obtained in inoculating them with m. leprae. they begin by reporting on the origin of the animals, how they were captured, and their efforts until they found the right kind of housing for the armadillos. they also tell of their attempts to develop the correct feeding procedures. the result is the feed used now in their laboratory: chicken feed mixed with bread, eggs, meat and milk. along with this, they report on the way ...	1980	7042562
combined chemotherapy against mycobacterium leprae in the mouse.		1980	7018423
identification of mycobacterium leprae: use of wall-bound mycolic acids.	a simple method for extraction and analysis of wall-bound mycolic acids from small samples of mycobacteria is described. separation of mycolic acid classes according to their functional groups by thin-layer chromatography showed a difference between mycobacterium leprae and a number of strains of acid-fast bacilli cultured from leprosy biopsies in vitro. this technique is proposed as a convenient preliminary test in the identification of possible cultures of m. leprae.	1980	7019389
leprosy in a chimpanzee. postmortem lesions.	a young (5-7 year old) male chimpanzee died 33 months after the first clinical manifestations of a naturally acquired disease that was similar to disseminated leprosy in man. at autopsy there were diffuse or multifocal histiocytic infiltrations of the skin, nasal mucosa, pharynx, lung interstitium, liver, spleen, sclera, testicles, adrenal glands, and peripheral lymph nodes. major nerves of the forearms had extensive fibrosis. there were large numbers of acid-fast bacilli (afb), many occurring a ...	1980	7193655
lucio's phenomenon.	a 38-year-old woman had diffuse, nonnodular, lepromatous leprosy and lucio's phenomenon. most cases of lucio's phenomenon have been reported to have a leukocytoclastic vasculitis as the underlying pathologic abnormality. in this patient, however, the histologic picture of an early lesion of lucio's phenomenon showed a milk, mononuclear cell infiltration, endothelial swelling, vascular thrombosis, and ischemic necrosis. lepra bacilli were abundant around nerves and blood vessels, and many were no ...	1980	7356353
borderline tuberculoid leprosy following bcg vaccination. a case report.	borderline tuberculoid leprosy was diagnosed clinically and histologically in a four year-old boy about 6 months after intradermal vaccination with bcg. his mother reported that a lesion began to appear above the vaccination site on the arm 2 weeks after the vaccination, and a second lesion appeared on the chin 2 months later. responses in the lymphocyte transformation test to sonicated mycobacterium leprae, bcg, and to ppd were consistent with a tuberculoid leprosy infection. precipitation of b ...	1981	7195879
detection of mycobacterial lipids in skin biopsies from leprosy patients.	thin-layer chromatography was used to compare lipid extracts from lepromatous skin biopsies with those from normal skin and from mycobacterium leprae purified from armadillo spleen. several lipids were found in infected skin which were absent from normal skin but corresponded to lipids present in the purified m. leprae. these included mycolic acids, a 6-deoxyhexose-containing lipid (glycolipid i) and a wax ester (possibly related to the mycobacterium tuberculosis wax, phthiocerol dimycocerosate) ...	1981	7196889
fatal reaction to dapsone during treatment of leprosy.	a burmese boy being treated with dapsone (diaminodiphenylsulfone [dds]), 100 mg daily, for lepromatous leprosy had a fatal reaction to the drug 3 weeks after therapy was started. the clinical symptoms and progression of illness conform well to a "dds syndrome" first described in the early 1950s. although the syndrome clinically resembles infectious mononucleosis, neither epstein-barr virus nor cytomegalovirus was implicated as an etiologic agent in this case. the syndrome has been recognized dur ...	1981	7235421
[pathomorphologic changes in the internal organs of intact and mycobacterium leprae-infected 9-banded armadillos].	the internal organs of nine-banded armadillos were examined histologically before and after inoculation of the animals with m. leprae. 3--4 months after the inoculation two animals developed tuberculoid structures in the lungs, liver and skin. a conclusion is made that experimental leprosy infection in nine-banded armadillos may run its course not only in the lepromatous but also in the tuberculoid form of the disease.	1981	7295954
[effect of antileprotic preparations on glycolytic processes in rats infected with human mycobacterium leprae].		1981	7314889
serology of leprosy. i. indirect hemagglutination test with stabilized sensitized red cells.	an indirect hemagglutination (iha) test has been described for the qualitative and quantitative detection of antibodies specific to mycobacterium leprae. aldehyde stabilized red cells were sensitized with a sonicate antigen prepared from m. leprae purified from armadillo liver. these cells were titrated against sera from patients with different types of leprosy, their healthy household contacts and patients with tuberculosis. specific antibodies were demonstrated in leprous sera by iha test afte ...	1981	7334779
[lipidic constituents of "mycobacterium leprae" isolated from experimentally infected armadillo (author's transl)].	mycobacterium leprae (obtained from experimentally infected armadillo) was submitted to saponification. the liposoluble part was methylated and fractionated by chromatographic methods. each fraction was studied by gas-liquid chromatography. cholesterol (from the infected host) and the main fatty acids were identified. mycolic acids were isolated, and their structures determined, using mass spectrometry. these structures are useful to make a comparison of m. leprae with some other mycobacteria. s ...	1981	7020522
paracrystalline inclusions in mycobacterium leprae.	the occurrence of paracrystalline inclusions of mycobacterium leprae infected with the mycobacteriophage d29 or treated with mitomycin c was reported before [5, 6]. in pursuing these studies we have now documented by electron micrography a number of paracrystals we thought sufficient to further describe these inclusions, and to show that they appeared to be formed in association with the intracellular membranous structures of the leprosy bacilli.	1981	7020523
adenosine triphosphate content in mycobacterium leprae. a brief communication.		1981	7019109
the 1980 journal--a perspective in leprosy.		1981	7019112
mycobacterium leprae and the host response.		1981	7040872
the lymphocyte transformation test in leprosy with special reference to its use in epidemiology.		1981	7040878
in vitro lymphocyte stimulation using a purified antigen of mycobacterium leprae and tuberculin ppd.		1981	7040879
subclinical infection in leprosy--its detection and control by fluorescent leprosy antibody absorption (fla-abs) test.		1981	7040880
radioimmunoassay and other serologic tests and their application in epidemiological work.		1981	7040881
leprosy--the source of infection and its mode of transmission.		1981	7040883
occurrence of non-cultivable acid-fast bacilli in the environment and their relationship to m. leprae.		1981	7040884
[t and b lymphoid subpopulations in patients with leprosy].		1981	7047979
isolation and identification of mycolic acids in mycobacterium leprae and mycobacterium lepraemurium.	mycolic acids with a characteristic structure were isolated by high performance-liquid-chromatography (hplc) and mass-spectrometry from a foot pad of a nude mouse inoculated with mycobacterium leprae. mycolic acids with the same structure were also obtained from mycobacteria collected from the liver of an armadillo with experimental leprosy. mycolic acids were isolated from mycobacterium lepraemurium grown both in vivo and in vitro and these mycolic acids had different structures from those of m ...	1981	7042604
the problem of dapsone-resistant leprosy.		1981	7042605
dapsone-resistant leprosy-the thelep approach.		1981	7042606
primary dapsone-resistant leprosy in cebu, philippines.	a survey of the prevalence of primary dapsone-resistant leprosy in cebu, philippines, has yielded an estimate of 3.6 per 100. fifty-three of 55 patients proved to have m. leprae fully susceptible to dapsone. the organisms of two patients multiplied in mice administered the minimal effective dose of dapsone; and those of one of these patients also multiplied in mice administered dapsone in a 10-fold larger dose.	1981	7042607
in vitro stimulation of human lymphocytes with mycobacterial antigens.		1981	7042608
incorporation of 3h-thymidine in mycobacterium leprae within differentiated human macrophages.	the factors influencing the incorporation of 3h-thymidine (3h-tdr) in the dna of mycobacterium leprae within macrophages derived from human blood have been evaluated. fifty strains of m. leprae derived from skin nodules of patients with lepromatous leprosy were studied for their ability to incorporate 3h-tdr. control macrophages of the same donor maintained alone, or with autoclaved m. leprae, showed low levels of baseline 3h-tdr incorporation. during a 15-day period of pulsing, 27 of the m. lep ...	1981	7021836
the uptake of 3h-thymidine in mycobacterium leprae inoculated mouse macrophage cultures as a rapid indicator of bacillary viability. factors influencing the specificity of the in vitro assay.	mouse peritoneal macrophages derived from balb/c were used as host cells for 17 human-derived m. leprae strains. simultaneous pulsing with 3h-thymidine (3h-tdr) showed uptake of the radiolabel in 58.8% of the bacilli over a 14 day period. preliminary data of three m. leprae strains indicate that the 3h-tdr is preferentially incorporated into the mycobacterial dna and not into the mammalian host cell dna. this in vitro assay provides a rapid assessment of m. leprae viability. the factors influenc ...	1981	7024148
a novel phenolic glycolipid from mycobacterium leprae possibly involved in immunogenicity and pathogenicity.	a phenolic glycolipid was obtained in high amounts (2% of dry weight) from mycobacterium leprae isolated from infected armadillo liver. infrared and nuclear magnetic resonance spectroscopy showed that it is closely related to "mycoside a" from mycobacterium kansasii and is therefore a glycosylphenolic phthiocerol diester. the crucial difference between the two products is in the composition of the attached trisaccharide. gas-liquid chromatography-mass spectroscopy showed that the product from m. ...	1981	7024248
a report on the biochemical analysis of mycobacterium w.	a mycobacterial strain known as mycobacterial strain w was analysed for its growth characteristics and biochemical traits. this strain was found to be a rapid grower, with luxurient growth on lowenstein-jensen medium, dubos agar, middlebrook's agar and sauton's medium. colonies were smooth, convex and nonpigmented. some of the colonies which appeared rough were similar to smooth colonies at least in biochemical characteristics. this organism was tolerant to wide range of temperatures and to chem ...	1981	7024633
discharge of m. leprae in milk of leprosy patients.	a quantitative estimation of discharge of m. leprae in the milk of 39 leprosy female patients has been made. twelve of the 39 patients (10 ll/bl and 1 each of t/bt and bb) showed bacilli in their milk. only one of these patients was on treatment. afb count in 10 ml of milk was found to range from 4.3 x 10(4) to 4.3 x 10(5). significance of discharge of such a large number of bacilli in breast milk is discussed.	1981	7024634
fourth annual report of the undp/world bank/who special programme for research and training in tropical diseases.		1981	7024635
the immunology of leprosy: speculations on the leprosy spectrum.	leprosy is a chronic infectious disease caused by mycobacterium leprae. the disease presents a wide spectrum of clinical manifestations, ranging from lepromatous to tuberculoid leprosy; each form may be punctuated by episodes of acute exacerbation, called reactional states. these polar forms and reactional states appear to be determined by immunologic interactions between the host and the microorganism. this review describes the different measurable parameters that permit the classification of a ...	1981	7025147
serology of leprosy. ii. demonstration of specific antibodies to mycobacterium leprae by counter current electrophoresis.	a rapid and simple method for the demonstration of specific antibodies to mycobacterium leprae using counter current electrophoresis (cce) has been described. three types of antigens (sonicate antigen, surface antigen and purified protoplasmic protein antigen) were prepared from purified m. leprae and tested against sera from patients with different types of leprosy, and their healthy household contacts, by cce. antibodies specific to m. leprae were demonstrated by cce after absorption of sera w ...	1981	7038297
skin test response to m. leprae precursor coccoid organisms in a high leprosy endemic area as compared to lepromin.	the paper describes the results on several hundred skin tests on humans using antigens from leprosy derived non-acid fast coccoid organisms, and a mycobacterium that grew out of one of the coccoid organisms. early skin test response to the cytoplasmic fraction of one of the leprosy derived, non-acid fast, coccoid organism was very highly correlated with standard lepromin.	1981	7038299
inhibition of foot pad multiplication of m. leprae in mice vaccinated with leproma derived m. leprae precursor coccoid organism and passive transfer of dth response to normal mice through infusion of splenic cells of such vaccinated mice.		1981	7038300
abstract of papers presented at the international symposium on mycobacterium leprae and other atypical mycobacteria.		1981	7038301
[terminal innervation in 3 cases of leprous neuropathy (author's transl)].	three cases of leprosy are reported. in all patients, the diagnosis was made by finding hansen bacilli in skin lesions, nasal secretions and ears, and the immunological typing of leprosy was established. conduction velocity of motor and sensory nerves was measured and muscle biopsies were obtained. histochemical typing of muscle fibers and measurement of the terminal innervation ratio of motor axon vitally stained with methylene blue were performed. a subclinical involvement of peripheral motor ...	1981	7038820
iga and igm antibodies against mycobacterium leprae in cord sera and in patients with leprosy: an indicator of intrauterine infection in leprosy.	a solid-phase radioimmunoassay was developed for demonstration and quantification of iga and igm anti-m. leprae antibodies. iga and igm anti-m. leprae antibodies were demonstrated in a lepromatous serum pool, in various amounts in individual patients with lepromatous leprosy, and in lower concentration in tuberculoid leprosy and non-leprosy controls. iga and igm anti-m. leprae antibodies were demonstrated in cord sera from babies of mothers with leprosy. the reliability of fetal iga and igm anti ...	1981	7038849
studies of lysosomal enzymes in macrophages. iii. lysosomal enzyme activities in cultured macrophages infected with some species of mycobacteria.		1981	7028685
[difference in infectivity of mycobacterium leprae from patients in immunosuppressed mice (author's transl)].		1981	7028712
[problems in designing a protocol for a leprosy vaccine trial. (author's transl)].	investigations carried out by the scientific working group on immunology of leprosy (immlep) have led to the planning of field trials of an experimental vaccine preparation to be carried out in few years' time. the objectives of these trials have been defined as follows: 1) the main objective should be the assessment of the protective effect of the vaccine against leprosy, its various forms and more particularly the lepromatous form. 2) another important objective will be to determine the value ...	1981	7029677
[studies on the lipids of "mycobacterium gordonae" in comparison with those of "m. leprae" and of some other scotochromogenic mycobacteria (author's transl)].	the mycolic acids were isolated from mycobacterium gordonae (strain atcc 14470), and purified by thin layer chromatography. three species were studied by mass spectrometry. the analogy between m. gordonae and m. leprae, based on the lack of tuberculostearic acid, was supported by the comparison of the structures of their mycolic acids. succinct analyses of the lipids of three other scotochromogenic strains of mycobacteria, using thin layer chromatography and gas-liquid chromatography, were perfo ...	1981	7030172
the mycobacterium smegmatis peroxidase, cross-reacting antigen with mycobacterium leprae.		1981	7030274
potential anti-leprosy vaccine from killed icrc bacilli--a clinicopathological study.		1981	7030942
the effect of body temperature and cell-mediated immunity on the growth of mycobacterium marinum and mycobacterium leprae in mice.	evidence is presented that the high susceptibility of armadillos to infection with mycobacterium leprae cannot be explained solely in terms of body temperature because mutant mice maintained with a body temperature similar to that of armadillos do not become heavily infected with m. leprae. the depression of cell-mediated immunity accompanying the low body temperature is not sufficient to produce an overwhelming infection. the results obtained with m. marinum suggest that whereas lack of cell-me ...	1981	7031255
a brief review of experiences with short-term clinical trials monitored by mouse-foot-pad-inoculation.		1981	7031389
pharmacologically-active mediators of hypersensitivity reactions in the blood of lepromatous patients with erythema nodosum leprosum.		1981	7031390
release of soluble factors from lymph nodes containing mycobacterial granulomas and their effect on fibroblast function in vitro.		1981	7032710
the effect of delayed addition of antigen and 'e' rosetting on the proliferative response to mycobacterial antigens of peripheral blood lymphocytes from normal individuals or from patients with tuberculosis or leprosy.	some suppressor cells are reported to lose their activity when precultured without stimulus in vitro. we have investigated the role of such suppressors in responsiveness to mycobacterial antigens of peripheral blood mononuclear cells (pbmnc) from patients with leprosy or tuberculosis, or from normal donors. delayed addition of mycobacterial antigens (mycobacterium leprae, mycobacterium vaccae and mycobacterium tuberculosis), but not of a fungal antigen (candida albicans) caused enhanced response ...	1981	7033116
relationship between t-cell population in neonatally thymectomized lewis rats and susceptibility to infection with mycobacterium leprae.	the neonatally thymectomized lewis rat (ntlr) is highly susceptible to infection with m. leprae. however, a significant percentage of ntlr respond to infection with m. leprae in much the same way as do intact rats, yet show no evidence of residual thymus. to determine whether there was a correlation between the number of remaining t-cells and susceptibility to infection with m. leprae, a direct fluorescent antibody test was performed using a highly specific, absorbed antithymocyte globulin label ...	1981	7033149
experimental mycobacterial granulomas in guinea pig lymph nodes: ultrastructural observations.	a systematic ultrastructural study has been performed of the mononuclear phagocytes in granulomas induced by different types of mycobacteria, e.g. live bcg (pasteur), irradiated m. leprae and irradiated bcg (pasteur) in guinea pig lymph nodes. live bcg (pasteur) induces a granuloma which peaks at 2-3 weeks and by light microscopy, a large number of the infiltrating cells have the appearance of epithelioid cells. ultrastructurally a large proportion of these cells have a distinct appearance. they ...	1981	7033491
[studies on m. leprae isolated from the lymph of a lepromatous patient using the freeze-fracture technic].		1981	7034060
induction of delayed-type hypersensitivity to mycobacterium leprae in healthy individuals.	delayed-type hypersensitivity (dth) to a soluble mycobacterium leprae skin test antigen (sml) was successfully induced in healthy volunteers following immunization with 2 x 10(8) killed armadillo-derived m. leprae. no better sensitization was obtained by a mixture of live bcg and killed m. leprae. the relative specificity of the dth reaction to sml has been demonstrated in this study, since little cross-reactivity was observed to ppd, after immunization with bcg ro m, leprae alone, or combined. ...	1981	7035030
failure to induce delayed-type hypersensitivity to mycobacterium leprae in long-term treated lepromatous leprosy patients.	lepromatous leprosy (ll) patients whose bacillary load has decreased to almost undetectable levels by long-term chemotherapy failed to develop delayed-type hypersensitivity (dth) to mycobacterium leprae antigen following immunization with killed armadillo-derived m. leprae. when these ll patients were immunization with killed armadillo-derived m. leprae. when these ll patients were immunized with killed m. leprae in a mixture with live bcg, only dth to purified protein derivative (ppd) was induc ...	1981	7035031
does the suppression of the immune response in pre-clinical lepromatous leprosy affect both cell-mediated and humoral responses in mycobacterium leprae antigens?		1981	7017316
activity of four clofazimine analogues against mycobacterium leprae.		1981	7017318
the mononuclear cell series in leprosy: an ultrastructural report.		1981	7017319
selective loss of mycobacterium leprae responsiveness of circulating lymphocytes in primary neuritic leprosy.		1981	7017320
bacterial persistence in leprosy.	bacterial persistence is the capability of microorganisms to survive in the host despite adequate antimicrobial treatment. this is a general phenomenon and has been observed during infection with a number of organisms. the subject is reviewed in relation to the treatment of leprosy. the presence of persisters in the large bacterial populations that exist in patients with lepromatous leprosy is not surprising. it is unlikely that in the near future a new drug or combination of drugs will be found ...	1981	6792087
the six disease of who.		1981	6794841
an overview of carville research.		1981	6797220
in vitro cultivation of m. leprae.		1981	6797806
typical or atypical mycobacteria?		1981	6801836
immunogenetics of susceptibility to leprosy, tuberculosis, and leishmaniasis. an epidemiological perspective.	the literature on the genetic regulation of susceptibility in leprosy, tuberculosis, amd leishmaniasis is critically reviewed. of the three groups of diseases, leprosy has received the most attention from the standpoint of human genetics. there is now evidence that genetic factors, some of them hla-linked, play a role in tuberculoid leprosy. however, the evidence leaves considerable room for environmental determinants in addition to genetic background. several twin studies of tuberculosis have f ...	1981	6804402
attempt to induce immune-tolerance to m. leprae in mice.		1981	7012440
pathologic changes in striated muscles in leprosy.	eighteen muscle biopsies from male adult patients of lepromatous and borderline lepromatous leprosy were studied. bi in the biopsies ranged from 1 + to 3+. there was conspicuous absence of granuloma except for a collection of foamy histiocytes in intermysial region and on two occasions in the intermysial nerve endings in ll patients. at places solid staining bacilli were seen in normal looking muscles. none of the biopsies showed loss of muscle striations, fatty change, sarcolemmal changes, fibr ...	1981	7012441
bcg induced immunity to mycobacterium leprae in mice.	one to three bcg vaccinations were given to various groups of mice--25 to 2 days preceding challenge with myco. leprae. harvesting of myco. leprae was done every month upto a period of 7 months. lymph node enlargement was taken as an indicator of successful vaccination. an increasing degree of protection was noted in bcg immunised mice as observed by a reduced number of bacilli harvested. protection afforded was proportional to the number of doses of bcg given.	1981	7012442
towards a vaccine against leprosy.		1981	7012444
[results of bacilloscopy practiced on 360 leprosy patients, treated for over 5 years by sulfone monotherapy and examined at bamako (mali)].		1981	6805233
[clinical and paraclinical aspects of secondary drug resistance to 4-4' diaminodiphenylsulfone in a population of leprosy patients in bamako, republic of mali].		1981	6805234
uptake of 3,4-dihydroxy[3h]phenylalanine by mycobacterium leprae isolated from frozen (-80 degrees c) armadillo tissue.	mycobacterium leprae separated from armadillo tissues stored at -80 degrees c is similar to that from human sources in its ability to take up 3h-labelled 3,4-dihydroxyphenylalanine (dopa). several inhibitors were studied which showed complete or partial inhibition of [3h]dopa uptake. these findings suggest that m. leprae isolated from frozen tissue possesses an active uptake system for [3h]dopa.	1981	6806435
bcg, corynebacterium parvum or mycobacterium leprae added to cultures of bcg-primed mouse spleen cells cause an enhanced primary antibody response in vitro.	a few weeks after mice were injected i.v. with 10(8) live mycobacterium bovis, bcg, the antibody response of their spleen cells to srbc in vitro was comparable with the response of cells from untreated mice. addition of bcg organisms to the culture vessels resulted in enhanced antibody-forming cell (afc) responses by the primed cells but not by the cells from the untreated mice. no evidence was found for a direct stimulation of b cells and cell depletion experiments suggested macrophages were di ...	1981	7007221
a claim for a mycobacterium leprae specific antigen.		1981	6788723
permeability of mycobacterium leprae to dapsone: alteration by purification procedures.	permeability of mycobacterium leprae to dapsone in vitro was determined by the ability of the drug to inhibit o-diphenoloxidase of the bacilli. dapsone showed little effect on the enzyme activity of the intact organisms. when the m. leprae preparations were washed with trypsin, naoh, or acetone and ether, dds penetrated the bacillus to inhibit its o-diphenoloxidase. the method might be useful in studying the utilization of added metabolites by purified m. leprae suspensions.	1981	6789004
[study of experimental leprosy in nude-mice inoculated with m. leprae--light and electron microscopic findings].		1981	6754678
the association of pregnancy and leprosy. ii. pregnancy in dapsone-resistant leprosy.		1981	7035779
activity of the combination of isoniazid, protionamide and dapsone against mycobacterium leprae and some other mycobacteria.	no additive effect was found at the addition of isoniazid to either dapsone or protionamide against m. leprae and against highly dapsone-sensitive in vitro growing mycobacterium sp. strains. it is concluded that the indication of the administration of isoniazid in leprosy is extremely limited and could only be envisaged as a companion drug in combined therapy of dapsone-resistant multibacillary leprosy in light-skinned people, when aminoglycosides cannot be administered.	1981	7037011
hypothesis: do phases of immunosuppression during a mycobacterium leprae infection determine the leprosy spectrum?		1981	6454034
inhibition of the proliferative response of peripheral blood lymphocytes to mycobacterial or fungal antigens by co-stimulation with antigens from various mycobacterial species.	soluble antigen preparations from mycobacterium leprae are reported to inhibit the response to other stimuli, of peripheral blood mononuclear cells from lepromatous leprosy cases (bl/ll) and also from tuberculoid cases (tt/bl) and normal donors. we confirm these findings and in addition, report that a similar suppressive effect is exerted by antigen from mycobacterium vaccae, mycobacterium nonchromogenicum and, to a lesser extent, mycobacterium tuberculosis and mycobactyerium kansasii. moreover, ...	1981	6459289
prothionamide and prothionamide-s-oxide in experimental leprosy.		1981	6459296
[reverse or inverse reaction - a present-day point].	the reversal or inversal reactions, well studied in some research centers, is still imperfectly known, even contested or denied. this lack of knowledge has been one of the hindrances in the understanding of the mechanism of the hansenic neuritis. the evolution of the concepts about this question is studied, starting with souza lima and souza campos (1950), followed by tajiri (1955), up to the present "i reaction" of jopling, involving the "up grading" and "down grading" reactions. an interpretat ...	1981	6749678