Publications

alterations in the phospholipid composition of rhodopseudomonas sphaeroides and other bacteria induced by tris.	alterations in the phospholipid head group composition of most strains of rhodopseudomonas sphaeroides, as well as rhodopseudomonas capsulata and paracoccus denitrificans, occurred when cells were grown in medium supplemented with tris. growth of r. sphaeroides m29-5 in tris-supplemented medium resulted in the accumulation of n-acylphosphatidylserine (naps) to as much as 40% of the total whole-cell phospholipid, whereas naps represented approximately 28 an 33% of the total phospholipid when r. c ...	1982	6982264
kinetic analysis of n-acylphosphatidylserine accumulation and implications for membrane assembly in rhodopseudomonas sphaeroides.	the accumulation of n-acylphosphatidylserine (naps) in response to the inclusion of tris in the growth medium of rhodopseudomonas sphaeroides strain m29-5 has been examined. in the accompanying paper (donohue et al., j. bacteriol. 152:000--000, 1982), we show that in response to tris, naps accumulated to as much as 40% of the total cellular phospholipid content. naps accumulation began immediately upon addition of tris and was reflected as an abrupt 12-fold increase in the apparent rate of naps ...	1982	6982265
essential sulfhydryl groups and the light-dependent transhydrogenase system of rhodopseudomonas spheroides: localization of substrate binding sites and evidence for masked or buried sulfhydryl groups in the peripheral protein factor.	the light-dependent transhydrogenase system of rhodopseudomonas spheroides which consists of a peripheral protein factor and a membrane-bound component contains essential sulfhydryl groups that are sensitive to p-hydroxymercuribenzoic acid (pmb). there are two types of sulfhydryl groups required for light-dependent (ld) transhydrogenation. one type is associated with the membrane-bound component and participates in or influences the binding of one of the substrates, nadh. a second type is associ ...	1982	6983381
short-term regulation of the nitrogenase activity in rhodopseudomonas sphaeroides.	the nitrogenase activity in whole cells of rhodopseudomonas sphaeroides could be inhibited by lowering the electrical potential across the cytoplasmic membrane. the membrane potential was partly dissipated either by lowering the light intensity or by the addition of a lipophilic cation, tetraphenylphosphonium. under these circumstances, it was shown that the intracellular atp/adp ratio was not affected and that the inhibition of the whole cell nitrogenase activity was not due to an inactivation ...	1982	6983438
purification and characterization of membrane-bound ferrochelatase from rhodopseudomonas sphaeroides.	ferrochelatase (protohaem ferro-lyase ec 4.99.1.1) has been purified to apparent homogeneity from the facultative photosynthetic bacterium rhodopseudomonas sphaeroides. the enzyme has been purified 1,640-fold with 43% recovery from isolated membrane fragments. the enzyme has a molecular weight of approximately 115,000 as estimated by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration chromatography through sephadex g-150 in the presence of 0.5% sodium deoxycholate. ...	1982	6983526
the use of n-methylprotoporphyrin dimethyl ester to inhibit ferrochelatase in rhodopseudomonas sphaeroides and its effect in promoting biosynthesis of magnesium tetrapyrroles.	n-methylprotoporphyrin dimethyl ester inhibits ferrochelatase in isolated membranes of rhodopseudomonas sphaeroides at low concentrations (around 10 nm). full inhibition developed after a short lag phase. the inhibition was non-competitive with porphyrin substrate. addition of inhibitor to growing cultures of rps. sphaeroides caused a decrease (near 40%) in cytochrome content and a severe inhibition of ferrochelatase; the excretion of haem into the medium by cell suspensions was also severely in ...	1982	6984332
neutron scattering studies of photosynthetic membranes in aqueous dispersion.		1982	6984710
reduction of cytochromes b6 and f in isolated plastoquinol-plastocyanin oxidoreductase driven by photochemical reaction centers from rhodopseudomonas sphaeroides.	photochemical reaction centers isolated from the bacterium rhodopseudomonas sphaeroides are able to donate electrons to cytochromes b6 and f in the plastoquinol,-platocyanin oxidoreductase isolated from spinach chloroplasts. the reduction reactions occur only after the second single turnover flash, in a reaction which is sensitive to inhibitors of the reactions in the chloroplast membranes. when all the components of the b6f complex are oxidized prior to activation, both cytochromes b6 and f are ...	1982	7037783
[stability of hydrogenase from the purple sulfur bacteria thiocapsa roseopersicina].	the hydrogenase from t. roseopersicina is highly resistant to the effects of urea (8 m), me2so (20%) and ds-na (1%), while inactivation of the hydrogenase from rhodopseudomonas capsulata occurs in the presence of 0.1% ds-na. the higher the purification level of t. roseopersicina hydrogenase preparation, the higher stability it possesses (t 1/2 = 60 days, 24 degrees). the hydrogenase inactivation at 80 degrees under anaerobic conditions occurs in one stage according to the equation of first-order ...	1982	7046814
recombination dynamics in bacterial photosynthetic reaction centers.	the time dependence of magnetic field effects on light absorption by triplet-state and radical ions in quinone-depleted reaction centers of rhodopseudomonas sphaeroides strain r-26 has been investigated. measurements on the time scale of the hyperfine interaction in the radical pair [(bchl)2+. ...bph-.)] provided kinetic data characterizing the recombination process. the results have been interpreted in terms of a recently proposed model that assumes an intermediate electron acceptor (close site ...	1982	7049260
peptidoglycan of rhodopseudomonas viridis: partial lack of n-acetyl substitution of glucosamine.	a lack of at least 70% of n-acetyl substitution of glucosamine in the glycan strands of the peptidoglycan from the gram-negative bacterium rhodopseudomonas viridis is reported. a disaccharide, very likely glcn beta(1 leads to 4) mur, was observed in hydrolysates of the isolated peptidoglycan. the disaccharide was not observed when peptidoglycan was n-acetylated before hydrolysis. the peptidoglycan of r. viridis was resistant to lysozyme but became sensitive after n-acetylation with acetic anhydr ...	1982	7054141
studies on the size and composition of the isolated light-harvesting b800-850 pigment-protein complex of rhodopseudomonas capsulata.		1982	7073287
polar lipids in phototrophic bacteria of the rhodospirillaceae and chromatiaceae families.	the polar lipids of photosynthetic purple bacteria of the genera chromatium, thiocapsa, thiocystis, ectothiorhodospira, rhodopseudomonas, rhodospirillum, and rhodomicrobium were analyzed. characteristic compositions of the polar lipids were found for most of the rhodospirillaceae and chromatiaceae species. phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin were the major phospholipids in most species. phosphatidylcholine was present as a major component in all species of the genus e ...	1982	7076618
photopigments in rhodopseudomonas capsulata cells grown anaerobically in darkness.	the phototrophic bacterium rhodopseudomonas capsulata can obtain energy for dark anaerobic growth from sugar fermentations dependent on accessory oxidants such as trimethylamine-n-oxide or dimethyl sulfoxide. cells grown for one to two subcultures in this fashion, with fructose as the energy source, showed approximately a twofold increase in bacteriochlorophyll content (per milligram of cell protein) and developed extensive intracytoplasmic membranes in comparison with cells grown photosynthetic ...	1982	7076623
absorption and fluorescence spectra of light-harvesting bacteriochlorophyll-protein complexes from rhodopseudomonas palustris in the near-infrared region.	bacteriochlorophyll (bchl)-protein complexes were isolated from rhodopseudomonas palustris. detergent treatment and polyacrylamide gel electrophoresis under rather mild conditions resulted in clear separation of light-harvesting bchls into b870 bhls and b800-850 bchls as two distinctly different protein complexes. it was shown that almost all the bchl contained in intracytoplasmic membranes was recovered as either b870-reaction center complexes or b800-850 complexes without loss of bchls as free ...	1982	7076642
circular dichroism of bacteriochlorophyll a in light-harvesting bacteriochlorophyll-protein complexes from rhodopseudomonas palustris.	bacteriochlorophyll (bchl)-protein complexes containing light-harvesting bchls were isolated from rhodopseudomonas palustris, and the cd spectra of these complexes were measured in the near-infrared region. these isolated bchl-protein complexes retained the cd signals of light-harvesting bchls that were observed in intracytoplasmic membrane preparation. comparison of the cd spectrum of b870-reaction center complexes with that of the isolated reaction centers revealed that the peak at 860 nm and ...	1982	7076643
the participation of the shemin and c5 pathways in 5-aminolaevulinate and chlorophyll formation in higher plants and facultative photosynthetic bacteria.	chlorophyll and bacteriochlorophyll formation from 14c-labelled precursors was studied during the illumination of etiolated maize leaves excised from dark-grown seedlings and in cell suspensions of respiring, dark-, aerobically-grown rhodopseudomonas spheroides adapting to the photosynthetic state in the light under anaerobic conditions. it was found that 1-14c-labelled glutamate and 2-oxoglutarate were incorporated into the tetrapyrrole moieties of chlorophyll and bacteriochlorophyll. this sugg ...	1982	7106702
synthesis of 3-o-methyl-l-xylose, a component of lipopolysaccharides of gram-negative bacteria.		1982	7127339
copurification of citrate lyase and citrate lyase ligase from rhodopseudomonas gelatinosa and subsequent separation of the two enzymes.	a procedure has been worked out which allowed the purification and crystallization of a citrate lyase/citrate lyase ligase complex from rhodopseudomonas gelatinosa. the complex was subsequently separated to yield two homogeneous enzymes. citrate lyase ligase was purified 365-fold with a yield of 3.23%. the molecular weight of the enzyme was estimated to be 39500, the enzyme consisted of one polypeptide chain. the reaction rates for atp, acetate and citrate lyase (sulfhydryl form) followed michae ...	1982	7128585
analysis of structure-function relationships in citrate lyase isolated from rhodopseudomonas gelatinosa as revealed by cross-linking and immunoelectron microscopy.	citrate lyase (ec 4.1.3.6) isolated from rhodopseudomonas gelatinosa was analyzed using cross-linking experiments and immunoelectron microscopy. different cross-linking reagents and antibodies directed against citrate lyase and specifically against all three subunit types (l, m and s) were applied. a structure-function model is proposed for citrate lyase from r. gelatinosa: the enzyme occurs in two configurations, 'rings' and 'stars'. the ring contains two identical layers each consisting of thr ...	1982	7128586
three-dimensional crystals of a membrane protein complex. the photosynthetic reaction centre from rhodopseudomonas viridis.		1982	7131557
respiratory control and the basis of light-induced inhibition of respiration in chromatophores from rhodopseudomonas capsulata.		1982	7138547
the polypeptide components from light-harvesting pigment-protein complex ii (b800-850) of rhodopseudomonas capsulata. solubilization, purification and sequence studies.	a new procedure for isolation, purification and identification of the three polypeptides of the membrane-bound light-harvesting complex ii (b800-850) of rhodopseudomonas capsulata has been developed. the polypeptides were extracted from crude intracytoplasmic membranes with chloroform/methanol/ammonium acetate and separated by chromatography on sephadex lh60. the peak fractions were transferred to solvents of different polarity and separated by gel filtration or ion-exchange chromatography. the ...	1982	7140771
the structure of the bacterial photosynthetic unit.		1982	7141087
on the extent of localization of the energized membrane state in chromatophores from rhodopseudomonas capsulata n22.	1. the principle of the double-inhibitor titration method for assessing competing models of electron transport phosphorylation is expounded. 2. this principle is applied to photophosphorylation by chromatophores from rhodopseudomonas capsulata n22. 3. it is found that, in contrast to the predictions of the chemiosmotic coupling model, free energy transfer is confined to individual electron transport chain and atp synthase complexes. 4. this conclusion is not weakened by arguments concerning, the ...	1982	7150247
comparative studies of protein properties and bacteriochlorophyll contents of bacteriochlorophyll-protein complexes from spectrally different types of rhodopseudomonas palustris.	the amino acid compositions, constituent polypeptides and bacteriochlorophyll (bchl) contents of two kinds of bchl-protein complexes isolated from rhodopseudomonas palustris were examined. spectrally dissimilar intracytoplasmic membranes obtained from cells cultured under different conditions were used as starting materials. the b870-reaction center complex was consistent in its amino acid composition, constituent polypeptides and bchl content, as well as in its near-infrared absorption spectrum ...	1982	7161260
amino-terminal sequences of the l, m, and h subunits of reaction centers from the photosynthetic bacterium rhodopseudomonas sphaeroides r-26.	we have determined the sequence of the 25-28 amino-terminal residues of the three subunits, l, m, and h, of the membrane-bound reaction center protein of the photosynthetic bacterium rhodopseudomonas sphaeroides r-26. the sequences are as follows: l, h2n-ala-leu-leu-ser-phe-glu-arg-lys-tyr-arg- val-pro-gly-gly-thr-leu-val-gly-gly-asn-leu-phe-asp-phe-(his)-val-; m, h2n-ala-glu-tyr-gln-asn-ile-phe-ser-gln-val-gln-val-arg-gly-pro-ala-asp-leu-gly-met-thr-glu-asp-val-asn-leu-ala-asn-; h, h2n-met-val- ...	1982	6753921
topography of reaction center subunits in the membrane of the photosynthetic bacterium, rhodopseudomonas sphaeroides.	the localization of the reaction center polypeptides (l, m, and h) in the membranes of both the wild-type, strain 2.4.1, and the carotenoidless mutant, r-26, of rhodopseudomonas sphaeroides was determined by using affinity-purified antibodies specific for these proteins. binding of the antibodies to reaction center subunits in spheroplasts was visualized in the electron microscope by immunoferritin labeling. the h and m subunits were labeled at both the cytoplasmic and the periplasmic surfaces o ...	1982	6754742
[hydrogenase activity of rhodopseudomonas capsulata growing on organic media].	when rhodopseudomonas capsulata b10 grows in media with different organic compounds, the hydrogenase activity estimated both by the evolution and uptake of h2 is lowest in cells taken from the middle of the exponential growth phase, and highest in cells from the beginning of the stationary phase. cells grown in a medium containing malate have a higher hydrogenase activity than those cultivated in a medium with lactate or other compounds (900 and 20 nmoles of h2 per 1 min per 1 mg of protein, res ...	1982	6755194
[absorption and fluorescence spectra and molecular organization of bacteriochlorophyll in reaction centers of rhodopseudomonas spheroides].	second derivative spectroscopy, computer curve analysis and stepanov's equation show that the absorbance and fluorescence spectra of primary electron donor in reaction center of rhodopseudomonas sphaeroides are splitting each into two asymmetric gaussian components. their absorption maxima at -196 degrees are 880 and 896 nm and emission maxima-906 and 923 nm, respectively. the absorption spectrum of bchl-800 splits in the near infrared region into two bands with maxima at 790 and 803 nm. these c ...	1982	6755225
[isolation of auxotrophic mutants of rhodopseudomonas sphaeroides].	a collection of stable auxotrophic mutants with a frequency of reversions to the wild type below 10(-8) was obtained from the wild strain of rhodopseudomonas sphaeroides 2r under the action of nitrosoguanidine or nitrosomethylurea. the mutants required methionine, leucine, arginine, histidine, tryptophan and cytosine for their growth. mutants carrying 2 or 3 auxotrophic labels in their genome were selected from the primary auxotrophs under the action of uv (lambda = 254 nm). the paper describes ...	1982	6757682
membrane differentiation and assembly of the pigment-protein complexes of the photosynthetic bacterium rhodopseudomonas capsulata.		1982	6761686
assembly and structural organization of pigment-protein complexes in membranes of rhodopseudomonas sphaeroides.	the b875 and b800-850 light-harvesting pigment-protein complexes of rhodopseudomonas sphaeroides are characterized further by lithium dodecyl sulfate/polyacrylamide gel electrophoresis at 4 degrees c. bacteriochlorophyll a was shown in reconstruction studies to remain complexed with its respective binding proteins during this procedure. from distributions in these gels, a quantitative description for the arrangement of the complexes is proposed. assembly of the complexes was examined in delta-am ...	1982	6761687
biosynthesis of the photosynthetic membranes of rhodopseudomonas sphaeroides.	the steady-state biosynthesis of the photosynthetic membrane (icm) of rhodopseudomonas sphaeroides has been reviewed. at moderate light intensities, 500 ft-c, preexisting icm serves as the insertion matrix for newly synthesized membrane components. whereas the bulk of the membrane protein, protein-pigment complexes, and pigments are inserted into preexisting icm throughout the cell cycle, phospholipid is transferred from outside the icm to the icm only at the time of cell division. because the s ...	1983	6607927
the complete amino-acid sequence of the large bacteriochlorophyll-binding polypeptide from light-harvesting complex ii (b800-850) of rhodopseudomonas capsulata.	the large bacteriochlorophyll-a-binding polypeptide of the light-harvesting complex ii (b800-850), having an apparent mr with sodium dodecyl sulfate/polyacrylamide electrophoresis of 10000, has been isolated and purified from intracytoplasmic membranes of the phototrophically negative mutant strain y5 of rhodopseudomonas capsulata. the primary structure of this polypeptide has been determined. the polypeptide consists of 60 amino acid residues yielding an mr of 7322. the hydrophobic stretch in p ...	1983	6825670
interaction between the respiratory and photosynthetic electron transport chains of intact cells of rhodopseudomonas capsulata mediated by membrane potential.	1. the inhibition of respiration by light and the relief from this inhibition by uncoupling agents have been studied in intact cells of rhodopseudomonas capsulata. 2. the same concentration range of carbonylcyanide p-trifluoromethoxyphenylhydrazone [cf3ophzc(cn)2] prevented light-inhibition of respiration and depressed the membrane potential (delta psi) in illuminated, aerobic bacterial suspensions. 3. for a wide range of cf3ophzc(cn)2 concentration the dependence of the respiration rate on delt ...	1983	6825711
structure of a bacterial photosynthetic membrane. isolation, polypeptide composition, and selective proteolysis.	a procedure for the isolation of highly purified bacterial photosynthetic membranes from rhodopseudomonas viridis is described. the purity of the final membrane fraction has been confirmed by electron microscopy. seven major polypeptide bands are associated with the photosynthetic membranes, and all seven are resistant to solubilization in triton x-100 detergent. two pigmented bands with apparent molecular weights of 44k and 41k are thought to be cytochromes. the three polypeptides with apparent ...	1983	6859862
oxygen-induced inhibition of light-dependent uptake of tetraphenylphosphonium ions as a probe of a direct interaction between photosynthetic and respiratory components in cells of rhodopseudomonas capsulata.	light-generated and oxygen-dependent membrane potentials by heterotrophycally grown cells of rhodopseudomonas capsulata have been investigated by using a tetraphenylphosphonium ion-selective electrode. the results show that respiratory electron transport affects the magnitude of photogenerated membrane potential while photosynthesis seems to either inhibit or stimulate respiration in coupled or uncoupled cells, respectively. these observations have been considered as evidence that the intracytop ...	1983	6860334
[nature of the long-wave absorption band of reaction centers in rhodopseudomonas viridis in relation to the process of primary charge separation].		1983	6861611
comparative nuclear magnetic resonance studies of high potential iron-sulfur proteins from chromatium vinosum and rhodopseudomonas gelatinosa. additional hyperfine shifted resonances and ph-dependent structural perturbations.	proton nmr spectra and their dependence on ph are reported for the oxidized and reduced forms of the high potential iron-sulfur proteins from chromatium vinosum and rhodopseudomonas gelatinosa. spectra of the protein from both species are very similar in the regions occupied by the hyperfine shifted resonances of protons located near the [fe4s4(s-cys)4] cluster. the oxidized proteins exhibit three new resonances that had not been previously detected, one at very low field (about 100 ppm) and two ...	1983	6863288
uncouplers can shuttle between localized energy-coupling sites during photophosphorylation by chromatophores of rhodopseudomonas capsulata n22.	two models of the action of uncoupler molecules in inhibiting photophosphorylation in bacterial chromatophores are considered: either uncoupler molecules shuttle rapidly between energy-coupling sites, or uncoupler molecules that are bound to particular sites in the chromatophores for a time that is comparable with the turnover time of the photophosphorylation apparatus may uncouple by a co-operative "substoichiometric' mechanism. it is found that the titre of uncoupler necessary to cause complet ...	1983	6870853
methylamine metabolism and its role in nitrogenase "switch off" in rhodopseudomonas capsulata.	in the photosynthetic bacterium rhodopseudomonas capsulata, nh4+ switch-off of nitrogenase activity can be mimicked by its analog, methylamine. like nh4+, methylamine appeared to require processing by glutamine synthetase (gs) before it was effective; gamma-glutamylmethylamide was shown to be the product of this reaction. evidence that this glutamine analog functioned directly to initiate nitrogenase inactivation was suggested first by the fact that it was a poor substrate for glutamate synthase ...	1983	6575733
[properties of two forms of ferredoxin from rhodopseudomonas capsulata].	electrophoretically homogenous preparations of two forms of ferredoxin were isolated from the nitrogen-fixing cells of the purple non-sulphur bacterium rhodopseudomonas capsulata b10. the values of mr for ferredoxins i and ii are 12000 and 18000, respectively. ferredoxin i contains 8 atoms of fe+2 and 8 atoms of s2-; ferredoxin ii--4 atoms of fe2+ and 4 atoms of s2- per molecule. the ferredoxins differ also in their absorption spectra, stability and catalytic activity during electron transfer to ...	1983	6575832
lipopolysaccharides of two strains of the phototrophic bacterium rhodopseudomonas capsulata.	the lipopolysaccharides of rhodopseudomonas capsulata strains st. louis (atcc 23782) and sp 11 both contain l-acofriose, rhamnose, glucose and glucosamine as the main sugar constituents. 2-keto-3-deoxyoctonate and neuraminic acid were tentatively identified. the fatty acid spectrum found with both strains comprises 3-oh-c10 and c12:1 (ester-linked) and 3-oxo-c14 (amide-linked). isolated lipid a from strain sp 11 contains glucosamine, glucosamine-phosphate and the total of the fatty acids of the ...	1983	6615126
o-acetylserine sulfhydrylase and s-sulfocysteine synthase activities of rhodospirillum tenue.	o-acetylserine sulfhydrylase in cell-free extracts of rhodospirillum tenue was markedly repressed after growth in the presence of sulfide or thiosulfate, whereas s-sulfocysteine synthase activity remained almost unchanged. purification on de52 cellulose resulted in the separation of two proteins: protein i with a molecular weight of 57000 had o-acetylserine sulfhydrylase activity only, while protein ii with a molecular weight of 46000 had s-sulfocysteine synthase activity in addition. the activi ...	1983	6615127
the effects of light and oxygen on membrane assembly in the photosynthetic bacteria.		1983	6617969
electron flow to dimethylsulphoxide or trimethylamine-n-oxide generates a membrane potential in rhodopseudomonas capsulata.	under dark and essentially anaerobic conditions electron flow to either dimethylsulphoxide or trimethylamine-n-oxide in cells of rhodopseudomonas capsulata has been shown to generate a membrane potential. this conclusion is based on the observation of a red shift in the carotenoid absorption band which is a well characterised indicator of membrane potential in this bacterium. the magnitude of the dimethylsulphoxide- or trimethylamine-n-oxide-dependent membrane potential was reduced either by a p ...	1983	6667089
purification and characterization of a dissimilatory nitrite reductase from the phototrophic bacterium rhodopseudomonas palustris.	a dissimilatory nitrite reductase from the facultatively phototrophic bacterium, rhodopseudomonas palustris strain 1a1 was studied. a basic level of the enzyme (10-50 mu/mg protein) was measured in dark, aerated and anaerobic, photosynthetic cultures. a marked derepression of enzyme synthesis occurred under conditions of oxygen limitation (200-300 mu/mg protein). the addition of nitrite (or nitrate) to the culture medium had only a slight effect on the maximal nitrite reductase titer of cells. t ...	1983	6670357
molecular properties of high potential iron sulfur protein of chromatium warmingii.	high potential iron sulfur protein (hipip) of the purple sulfur bacterium chromatium warmingii was purified to homogeneity by ion exchange chromatography, gel filtration and ammonium sulfate fractionation. the acidic protein was isolated in the reduced form. the best purity index (a280/a388) obtained was 2.52, and 3.8 mumol of the protein was isolated out of 100 g wet cell material. the molecular weights estimated by sodium dodecylsulfate polyacrylamide gel electrophoresis and gel filtration thr ...	1983	6670359
membrane association of the carbon monoxide oxidation system in rhodopseudomonas gelatinosa.	a comparison of the distribution of co oxidation activity between soluble and particulate protein fractions obtained after disruption of co-grown rhodopseudomonas gelatinosa 1 by french pressure cell breakage and osmotic lysis of spheroplasts suggested that, in situ, the enzyme complex was associated with the cell membrane. an improved, strictly anaerobic method is given for spectrophotometric measurement of co oxidation activity based on the carbon monoxide:methyl viologen oxidoreductase reacti ...	1983	6687360
metabolism of carbon monoxide by rhodopseudomonas gelatinosa: cell growth and properties of the oxidation system.	rhodopseudomonas gelatinosa 1 grew as an anaerobic facultative methylotroph with carbon monoxide as the sole carbon and energy source. carbon from co was assimilated into cell material via the ribulose 1,5-bisphosphate carboxylase cycle. the co oxidation system in r. gelatinosa was induced during growth with the gas substrate. light-grown cells did not oxidize co. surprisingly, when strain 1 cells grown in the dark with co were transferred to growth with both co and light, they continued to use ...	1983	6688413
kinetic studies on the membrane-bound and the purified coupling factor-atpase from rhodopseudomonas sphaeroides.	the activity of membrane-bound and purified atpase (ec 3.6.1.3) was potentiated by several divalent cations. highest rates of atp hydrolysis were obtained when the activity was measured with the (cation-atp)2- complex. free atp and free divalent cations in excess were found to be competitive inhibitors to the complex. the apparent km (complex) values were lower than the ki values for free atp indicating that the (cation-atp)2- complex is bound more tightly to the enzyme than the free atp. based ...	1983	6223582
overproduction of nitrogenase by nitrogen-limited cultures of rhodopseudomonas palustris.	rhodopseudomonas palustris cells grown on limiting nitrogen produced four- to eightfold higher nitrogenase specific activity relative to cells sparged with n2. the high activity of n-limited cells was the result of overproduction of the nitrogenase proteins. this was shown by four independent techniques: (i) titration of the mo-fe protein in cell-free extracts with fe protein from azotobacter vinelandii; (ii) direct detection of the subunits of mo-fe protein by sodium dodecyl sulfate-polyacrylam ...	1983	6402491
hybridization of cloned rhodopseudomonas capsulata photosynthesis genes with dna from other photosynthetic bacteria.	the homology of rhodopseudomonas capsulata dna segments carrying photosynthesis genes with sequences present in total dna from certain other photosynthetic and non-photosynthetic bacterial species was determined by hybridization. r. capsulata dna fragments that carry loci for production of peptide components of the photosynthetic reaction center and light-harvesting i antenna complex were found to hybridize to dna from some photosynthetic species. however, fragments that carry carotenoid or bact ...	1983	6406432
transcriptional regulation of several genes for bacteriochlorophyll biosynthesis in rhodopseudomonas capsulata in response to oxygen.	although it has been shown that bacteriochlorophyll synthesis in rhodopseudomonas capsulata is repressed by oxygen and high light intensity, few details of regulation by these environmental factors are known, primarily owing to a lack of assays for the biosynthetic enzymes. we have examined regulation at the transcriptional level by isolating and studying fusions between the mu d1(apr lac) phage and various bch genes. in these strains, the lacz gene of the phage is under the control of bch gene ...	1983	6415036
the effect of iron-hexacyanide binding on the determination of redox potentials of cytochromes and copper proteins.	the midpoint redox potentials of pseudomonas aeruginosa cytochrome c-551 and rhodopseudomonas viridis cytochrome c2 were measured as a function of ph in the presence of euglena cytochrome c-558 and the results compared with those obtained in the presence of ferro-ferricyanide. the pattern of ph dependence observed for the two bacterial cytochromes was the same whether it was measured by equilibrium with another redox protein or with the inorganic redox couple. thus, the ph dependence of redox po ...	1983	6418204
uptake of methionine sulfoximine by some n2 fixing bacteria, and its effect on ammonium transport.	the n2 fixing bacteria klebsiella pneumoniae, azospirillum brasilense, rhodopseudomonas sphaeroides and rhodospirillum rubrum, but not azotobacter vinelandii accumulate the glutamine analogue methionine sulfoximine in the cell. in the accumulating cells methionine sulfoximine inhibits ammonium transport. accumulation and inhibition are prevented by glutamine.	1983	6418571
different polysaccharides in the external layers (capsule and slime) of the cell envelope of rhodopseudomonas capsulata sp11.	two different acidic polysaccharides (i and ii) were detected in the external cell envelope layers (slime and capsule) of rhodopseudomonas capsulata sp11. polysaccharide i contains rhamnose, fucose, glucosamine and an unknown acidic sugar, it represents the slime material of the strain. polysaccharide ii contains rhamnose, galactose, 3-amino-3,6-dideoxygalactose, an unknown amino sugar and galacturonic acid, it represents very likely the capsule of r. capsulata sp11. polysaccharide i has a serol ...	1983	6421260
light-dependent regulation of the synthesis of soluble and intracytoplasmic membrane proteins of rhodopseudomonas sphaeroides.	cells of rhodopseudomonas sphaeroides grown under saturating light conditions (30 w/m2) and then shifted to low light intensity (3 w/m2) required 2.5 h to adapt to the new lower light conditions. after the shift, cell growth, whole cell protein accumulation, and bacteriochlorophyll accumulation ceased immediately. approximately midway into the adaptation period, bacteriochlorophyll synthesis commenced at a new, higher rate, which continued through the beginning of the low-light growth period unt ...	1983	6600232
long-chain fatty acid assimilation by rhodopseudomonas sphaeroides.	exogenously supplied long-chain fatty acids have been shown to markedly alleviate the inhibition of phototrophic growth of cultures of rhodopseudomonas sphaeroides caused by the antibiotic cerulenin. monounsaturated and polyunsaturated c18 fatty acids were most effective in relieving growth inhibition mediated by cerulenin. medium supplementation with saturated fatty acids (c14 to c18) failed to influence the inhibitory effect of cerulenin. the addition of mixtures of unsaturated and saturated f ...	1983	6600450
the transmembrane electrical potential in rhodopseudomonas sphaeroides determined from the distribution of tetraphenylphosphonium after correction for its binding to cell components.		1983	6600680
[mössbauer spectroscopy of intramolecular mobility in chromatophores from rhodopseudomonas spheroides].	mobility of the mössbauer label attached to the membrane proteins and the mössbauer probe embedded into the lipid matrix of the bacterial chromatophores were studied. positive correlation was established between the dynamic properties of hydrophobic compartments in the chromatophores and functional electron--transport activity at the level of quinone cofactors associated with the photosynthetic reaction centres.	1983	6600939
redox potential dependence of photophosphorylation and electron transfer in continuous illumination of rhodopseudomonas sphaeroides chromatophores.	the dependence on redox potential (eh) of the steady-state photophosphorylation rate in chromatophores of rhodopseudomonas sphaeroides ga was measured using slowly equilibrating (and hence less interfering) redox mediators. the remaining interference of the mediators was taken into account by extrapolating to zero mediator concentration. the extents of cytochrome redox reactions (in the presence of antimycin) and of the carotenoid shift were similarly measured. the redox titration of cytochrome ...	1983	6601471
physical and chemical characterization of the major outer membrane protein of rhodopseudomonas sphaeroides.	the characterization of the major outer membrane protein of rhodopseudomonas sphaeroides is described. molecular weight estimations using ferguson plots derived from sodium dodecyl sulfate and urea-polyacrylamide gels were 39,500 and 32,200, respectively, in good agreement with the value of 33,800 obtained from amino acid compositional studies. nh2-terminal amino acid determinations of the major outer membrane protein revealed a blocked nh2 terminus. gas chromatography of the acid-hydrolyzed pro ...	1983	6602128
nontoxic lipopolysaccharide from rhodopseudomonas sphaeroides atcc 17023.	chemical analysis of the lipopolysaccharide from rhodopseudomonas sphaeroides atcc 17023, isolated by the phenol-chloroform-petroleum ether method, revealed the presence of glucuronic acid, 2-keto-3-deoxyoctonate, threonine, and phosphorus in the polysaccharide moiety. the lipid a component contained glucosamine, glucosamine phosphate, amide-bound 3-oxotetradecanoic acid and 3-hydroxytetradecanoic acid, and ester-bound 3-hydroxydecanoic acid and 7-tetradecenoic acid. structural similarity of the ...	1983	6602801
accumulation and lethal effect of tritium (tritiated water) in rhodopseudomonas spheroides. under light-anaerobic and dark-aerobic conditions.	nonsulfur purple photosynthetic bacteria, rhodopseudomonas spheroides cells were cultured in medium containing tritiated water (tho) under the light-anaerobic and dark-aerobic conditions. the experimental r value defined as specific activity ratio of organic bound 3h to tho in medium was 0.49 and 0.48 for the light-anaerobically grown cells and the dark-aerobically grown cells, respectively. from the relation of r value to number of weight doubling of the cells (n), ratio of experimental r to th ...	1983	6602996
anaerobic and aerobic coproporphyrinogen iii oxidases of rhodopseudomonas spheroides. mechanism and stereochemistry of vinyl group formation.	an improved method for the preparation of various species of porphobilinogen stereospecifically labelled with 3h in the side chains (at c-6, c-7 and c-8) is described. these labelled samples were used to study the mechanism and stereochemistry of anaerobic as well as aerobic coproporphyrinogen iii oxidase of light-grown rhodopseudomonas spheroides. it was shown that both the oxidases catalyse the conversion of the propionate side chains of coproporphyrinogen iii into the vinyl groups of protopor ...	1983	6603215
light-mediated regulation of phospholipid synthesis in rhodopseudomonas sphaeroides.	the relationship between the culture levels of guanosine-5'-diphosphate-3'-diphosphate (ppgpp) and the rates of synthesis and accumulation of cellular phospholipids was examined in cultures of rhodopseudomonas sphaeroides that had been subjected to immediate decreases in incident light intensity. after a high-to-low light transition of high-light-adapted cells, an immediate inhibition of total cellular phospholipid production occurred coincident with a rapid accumulation of culture ppgpp. the in ...	1983	6603457
changes in the cytochrome composition of rhodopseudomonas sphaeroides grown aerobically, photosynthetically and on dimethyl sulphoxide.	several strains and mutants of rhodopseudomonas sphaeroides can be grown anaerobically in the dark in the presence of dimethyl sulphoxide as an electron acceptor. during adaptation to this fermentative mode of growth, two major c-type cytochromes are synthesized, one with mr 45 000 and the second with mr 20 000 and a midpoint potential of +120 mv. these cytochromes are barely detectable in membranes prepared from cells grown in aerobic or photosynthetic conditions. an electrophoretic method is p ...	1983	6603837
structural studies on the non-toxic lipid a from rhodopseudomonas sphaeroides atcc 17023.	the lipid a isolated from rhodopseudomonas sphaeroides atcc 17023 has been investigated. a sequence of analyses indicated beta-d-glucosaminyl-1,6-d-glucosamine as the sugar backbone carrying phosphate groups at c-1 of the reducing glucosamine and c-4 of the non-reducing glucosamine. both 3-(7-tetradecenoyl)oxytetradecanoic and 3-oxotetradecanoic acid are linked to the nh2 groups. two residues of 3-hydroxydecanoic acid are linked to hydroxy groups at c-3 of both reducing and non-reducing glucosam ...	1983	6604629
in vivo metabolic intermediates of phospholipid biosynthesis in rhodopseudomonas sphaeroides.	the in vivo metabolic pathways of phospholipid biosynthesis in rhodopseudomonas sphaeroides have been investigated. rapid pulse-chase-labeling studies indicated that phosphatidylethanolamine and phosphatidylglycerol were synthesized as in other eubacteria. the labeling pattern observed for n-acylphosphatidylserine (naps) was inconsistent with the synthesis of this phospholipid occurring by direct acylation of phosphatidylserine (ps). rather, naps appeared to be kinetically derived from an earlie ...	1983	6604726
mechanism of the activation of delta-aminolevulinate synthetase in rhodopseudomonas spheroides by rat liver mitochondrial fraction.		1983	6604727
heat modifiability and detergent solubility of outer membrane proteins of rhodopseudomonas sphaeroides.	the outer membrane fraction from rhodopseudomonas sphaeroides was isolated by isopycnic density centrifugation. the purity of this fraction was assayed by several methods. when the outer membrane fraction obtained after french press lysis of cells was compared with the outer membrane fragments released during spheroplast formation, the polypeptide profiles were identical. detergent solubilization of membrane fractions showed that triton x-100 nonselectively solubilizes both the cytoplasmic membr ...	1983	6605339
control of the formation of bacteriochlorophyll, and b875- and b850-bacteriochlorophyll complexes in rhodopseudomonas sphaeroides mutant strain h5.	rhodopseudomonas sphaeroides mutant h5 lacking 5-aminolevulinic acid synthase was employed to study the control of the formation of total bacteriochlorophyll as well as of the b875- and b850-bacteriochlorophyll protein complexes. the organisms were grown phototrophically in a chemostat where cell protein formation was limited by iron ions and bacteriochlorophyll by 5-aminolevulinic acid. 0.07 mol of bacteriochlorophyll was formed per mol of 5-amino-levulinic acid consumed. this stoichiometric re ...	1983	6607717
inhibition of electron transfer by 3-alkyl-2-hydroxy-1,4-naphthoquinones in the ubiquinol-cytochrome c oxidoreductases of rhodopseudomonas sphaeroides and mammalian mitochondria. interaction with a ubiquinone-binding site and the rieske iron-sulfur cluster.	3-alkyl-2-hydroxy-1,4-naphthoquinones (alkyl-hnq) inhibit rieske iron-sulfur cluster (rieske fes) oxidation and cytochrome b reduction in ubiquinol-cytochrome c oxidoreductase. the effects are the same as those of 5-undecyl-6-hydroxy-4,7-dioxobenzothiazole. concentrations for 50% inhibition in chromatophores of rhodopseudomonas sphaeroides (at 0.4 microm reaction center) are 2 microm for undecyl-, 3 microm for octyl-, and 40 microm for pentyl-substituted hydroxynaphthoquinones. the ethyl-substit ...	1983	6296106
proton nuclear magnetic resonance studies of the ligation states of the monomeric ferricytochrome c' from rhodopseudomonas palustris. modulation of axial histidine bonding via variable proton donation.	the monomeric ferricytochrome c' from rhodopseudomonas palustris strain 37 has been examined by 1h nmr spectroscopy at 45 degrees c and 360 mhz and at 55 degrees c and 200 mhz in 2h2o. the ph-dependent characteristics of the spectra have been analyzed in terms of two pk values at approximately 6 and approximately 8, the latter of which appears to correspond to the previously observed transition between an acidic species and a neutral species for this class of proteins. previously determined rate ...	1983	6296112
[various properties of delta-aminolevulinate synthetase from rhodopseudomonas palustris].	the enzymatic formation of delta-aminolevulinic acid (ala) in extracts of rhodopseudomonas palustris has been studied. some properties of delta-aminolevulinate synthetase (ala-s), including molecular weight km for glycine and the inhibition of enzyme activity by ala are reported.	1983	6336569
chemical cross-linking studies of the light-harvesting pigment-protein complex b800-850 of rhodopseudomonas capsulata.	the spatial relationship of the three polypeptides contained in the b800-850 light-harvesting complex of rhodopseudomonas capsulata has been studied with chemical cross-linking of crude membrane preparations of the phototrophic negative mutant strain y5. samples were cross-linked with the cleavable reagent dithiobis (succinimidyl propionate) (1.1 nm chain length) and analyzed by two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. membranes labelled with 14c-amino acids wer ...	1983	6339241
isolation and characterization of enhanced fluorescence mutants of rhodopseudomonas capsulata.	after enrichment by a tetracycline suicide under conditions nonpermissive for the growth of mutants defective in photosynthesis, colonies were screened for enhanced fluorescence in near-infrared light by using high-speed infrared photography. twenty mutants were isolated, and the chromatophore membranes were analyzed by a new, rapid microprocedure that revealed many different phenotypes among the mutants. the enhanced fluorescence mutants typically possessed a functional light-harvesting ii ante ...	1983	6341361
spatial differentiation in photosynthetic and non-photosynthetic membranes of rhodopseudomonas palustris.	the cytoplasmic membrane and the photosynthetic intracytoplasmic membranes of rhodopseudomonas palustris are spatially differentiated into regions of extremely high intramembrane-particle density (4,400 to 9,800/micron 2) and areas of lower intramembrane-particle density (2,700 to 5,900/micron 2). the high intramembrane-particle-density areas were always seen in association with photosynthetic membrane stacks. this differentiation was also seen in those areas of the cytoplasmic membrane which ad ...	1983	6343353
purification and characterization of a bifunctional l-(+)-tartrate dehydrogenase-d-(+)-malate dehydrogenase (decarboxylating) from rhodopseudomonas sphaeroides y.	a bifunctional enzyme, l-(+)-tartrate dehydrogenase-d-(+)-malate dehydrogenase (decarboxylating) (ec 1.1.1.93 and ec 1.1.1. . . , respectively), was discovered in cells of rhodopseudomonas sphaeroides y, which accounts for the ability of this organism to grow on l-(+)-malate. the enzyme was purified 110-fold to homogeneity with a yield of 51%. during the course of purification, including ion-exchange chromatography and preparative gel electrophoresis, both enzyme activities appeared to be in ass ...	1983	6345505
comparison between direct methods for determination of microbial cell volume: electron microscopy and electronic particle sizing.	size frequency distributions of different phototrophic and heterotrophic microorganisms were determined by means of scanning and transmission electron microscopy and electronic particle sizing. statistically significant differences existed among the three techniques used in this study. cells processed for electron microscopy showed lower mean cellular volumes than those processed for electronic particle sizing, reflecting a shrinkage by factors ranging from 1.1 to 6.2 (mean, 2.3). processing of ...	1983	6347064
two-dimensional crystals formed from photosynthetic reaction centers.	photosynthetic reaction centers from the bacterium rhodopseudomonas viridis were prepared after detergent solubilization of photosynthetic membranes. the purified reaction centers, in agreement with reports from other laboratories, contain four distinct polypeptides ranging in molecular weight from 28,000 to 41,000. when the detergent was gradually removed by dialysis under appropriate conditions, large two-dimensional sheets of reaction centers were formed, suitable for analysis by electron mic ...	1983	6352711
molecular-organization and biosynthesis of pigment-protein complexes of rhodopseudomonas capsulata.	the photosynthetic apparatus of the facultative phototrophic bacterium rhodopseudomonas capsulata contains three bacteriochlorophyll-carotenoid-protein complexes: the reaction center and the light-harvesting (lh) antenna complexes lhi (b870) and lhii (b800--850). in contrast to green anoxygenic phototrophic bacteria and the oxygenic cyanobacteria, the light-harvesting complexes of rhodospirillaceae and chromatiaceae are integral membrane particles. variations in light fluxes induce membrane diff ...	1983	6357026
the lactose carrier of escherichia coli functionally incorporated in rhodopseudomonas sphaeroides obeys the regulatory conditions of the phototrophic bacterium.	rhodopseudomonas sphaeroides was provided with the ability to transport lactose via conjugation with a strain of escherichia coli bearing a plasmid containing the lactose operon (including the lac y gene, coding for the lactose carrier or m protein) and subsequent expression of the lac operon in rps. sphaeroides (nano, f.e. and kaplan, s. submitted). the initial rate of lactose transport in rps. sphaeroides was studied as a function of the light intensity and the magnitude of the proton-motive f ...	1983	6360711
[resonance raman scattering of metal-containing proteins].		1983	6361878
biosynthesis of bacterial glycogen: activator specificity of the adpglucose pyrophosphorylase of rhodopseudomonads.	the adenosine diphosphate glucose pyrophosphorylases from rhodopseudomonas acidophila, rhodopseudomonas blastica, rhodopseudomonas globiformis, and rhodopseudomonas viridis were purified to the extent that their regulatory properties could be studied. with the exception of the r. viridis enzyme, all the enzymes could be activated by pyruvate or its analog, oxamate. the most effective activator for all the enzymes was fructose 6-p. however, the r. globiformis and r. viridis adp glucose pyrophosph ...	1983	6297405
interaction of cytochrome c with reaction centers of rhodopseudomonas sphaeroides r-26: localization of the binding site by chemical cross-linking and immunochemical studies.	the location of the cytochrome binding site on the reaction center of rhodopseudomonas sphaeroides was studied by two different approaches. in one, cross-linking agents, principally dithiobis(propionimidate) and dimethyl suberimidate, were used to link cytochrome c and cytochrome c2 to reaction centers; in the other, the inhibition of electron transfer by antibodies against the subunits was investigated. cytochrome c (horse) cross-linked to the l and m subunits, whereas cytochrome c2 (r. sphaero ...	1983	6297545
individual 1h-nmr assignments for the heme groups and the axially bound amino acids and determination of the coordination geometry at the heme iron in a mixture of two isocytochromes c-551 from rhodopseudomonas gelatinosa.	this paper describes chemical and physicochemical studies of two small isocytochromes c-551 (approx. 9000 dalton) from rhodopseudomonas gelatinosa. in spite of numerous amino acid substitutions in the n-terminal half of the sequence the two isoproteins could not be separated by the procedures used, presumably because they have identical size, charge and isoelectric points. individual assignments of the 1h-nmr lines of heme c and the axial ligands to the heme iron were therefore obtained by nucle ...	1983	6297597
the role of the proton-motive force and electron flow in light-driven solute transport in rhodopseudomonas sphaeroides.	the initial rate of uptake of alanine was studied in whole cells of rhodopseudomonas sphaeroides under conditions such that the proton-motive force generated by light-induced cyclic electron transfer, was composed of the membrane potential only. the proton-motive force was varied by varying the light intensity or by pretreatment of the cells with the uncoupler carbonylcyanide p-trifluoromethoxyphenylhydrazone. at constant light intensity a threshold delta psi is required before alanine uptake oc ...	1983	6297888
the influence of the ionic conductance on the relation between electron transport and proton-motive force in intact cells of rhodopseudomonas capsulata.	1. the dependence of membrane potential (delta psi) on the rate of respiration in darkened intact cell suspensions of rhodopseudomonas capsulata was distinctly non-linear: severe inhibition of respiration with either rotenone or kcn led to only a small drop in delta psi. 2. in the presence of 0.3 microms carbonylcyanide p-trifluoromethoxyphenylhydrazone [cf3ophzc(cn)2] the dependence of delta psi on respiratory rate became linear. consequently, and particularly at lower concentrations of cf3ophz ...	1983	6297908
characterization of a new membrane-bound cytochrome c of rhodopseudomonas capsulata.	a cytochrome c (cyt. c) was solubilized with triton-x-100 and co-purified with cytochrome c oxidase from membranes of chemotrophically grown cells of rhodopseudomonas capsulata. cyt. c and cytochrome oxidase were separated on sephadex g-50 columns. antibodies against cytochrome c2 from the same bacterium did not cross react with the membrane-bound cyt. c. the iep of the membrane-bound cyt. c was found to be ph 8.2, the midpoint potential was 234 +/- 11 mv at ph 7.0. this cyt. c binds co. the nat ...	1983	6298000
characterization of cytochrome b in the isolated ubiquinol-cytochrome c2 oxidoreductase from rhodopseudomonas sphaeroides ga.	extinction coefficients for cytochrome b and c1 in the isolated cytochrome bc1 complex from rhodopseudomonas sphaeroides ga have been determined. they are 25 mm-1 . cm-1 at 561 nm for cytochrome b and 17.4 mm-1 . cm-1 at 553 nm for cytochrome c1, for the difference between the reduced and the oxidized state. cytochrome b is present in two forms in the complex. one form has an em7 of 50 mv, an alpha-peak of 557 nm at liquid n2 temperature and of 561 nm at rt, which is red-shifted by antimycin a. ...	1983	6298005
alignment of genetic and restriction maps of the photosynthesis region of the rhodopseudomonas capsulata chromosome by a conjugation-mediated marker rescue technique.	the restriction map of a 46-kilobase fragment of the rhodopseudomonas capsulata chromosome was aligned with the genetic map of the photosynthesis region of that chromosome by a marker rescue technique. marker rescue was effected by mobilization of vectors bearing fragments of r. capsulata dna from escherichia coli to a set of r. capsulata mutants. plasmids pdpt51 and pdpt55 were constructed to mediate the intergeneric mobilization of pbr322 derivatives, and a mutant of r. capsulata with improved ...	1983	6302077
the existence of an antimycin a insensitive ubiquinol-cytochrome c reductase activity in the photosynthetic apparatus.	a nonproteinaceous, antimycin a insensitive ubiquinol-cytochrome c reductase activity is detected in and purified from chromatophores of rhodopseudomonas sphaeroides, r-26. this activity is about 5 times the antimycin a sensitive reductase activity in chromatophores and the two are not interconvertable. the purification involved chloroform:methanol (2:1), and hexane extractions and florisil column chromatography. the purified preparation contains some bacteriochlorophyll-like pigments and phosph ...	1983	6303319
nitrogen fixation and ammonia switch-off in the photosynthetic bacterium rhodopseudomonas viridis.	rhodopseudomonas viridis atcc 19567 grows by means of nitrogen fixation in yeast extract-n2 or nitrogen-free medium when sparged with 5% co2 and 95% n2 in the light at 30 degrees c. acetylene reduction assays for nitrogenase activity revealed an initially high level of activity during early-logarithmic growth phase, a lower plateau during mid- to late-logarithmic phase, and a dramatic reduction of activity at the beginning of the stationary phase. when viewed by electron microscopy, nitrogen-fix ...	1983	6305906
a new spatial structure for the axial methionine observed in cytochrome c5 from pseudomonas mendocina. correlations with the electronic structure of heme c.	cytochrome c5 from pseudomonas mendocina has been isolated and the coordination geometry at the heme iron was investigated by 1h nuclear magnetic resonance and circular dichroism spectroscopy. individual assignments were obtained for heme c and the axial ligands. from studies of nuclear overhauser enhancements the axial histidine imidazole ring orientation relative to the heme group was found to coincide with that of other c-type cytochromes. in contrast, a new structure was observed for the axi ...	1983	6309240
the amino acid sequence of cytochrome c-556 from agrobacterium tumefaciens strain apple 185.	the evidence for the amino acid sequence of cytochrome c-556 from agrobacterium tumefaciens strain apple 185 is reported. the sequence was determined by manual edman degradation of tryptic and chymotryptic peptides using the dabitc/pitc double-coupling method; some peptides were further cleaved by partial acid hydrolysis and with staphylococcus aureus protease. the sequence overlaps 13-15, 83-85 and 106-108 as well as the region 113-118 involving the haem-binding sequence cys-xaa-xaa-cys-his wer ...	1983	6309523
characteristics and amino-acid composition of a c-type cytochrome in electron acceptor function during thiosulfate-linked photoautotrophic growth of rhodopseudomonas palustris.	the purple bacterium rhodopseudomonas palustris (rhodospirillaceae) was grown in the light with thiosulfatee as the only electron source and hco theta 3/co2 as carbon requirement. during thiosulfat oxidation, photolithoautotrophically growing cells transferred the electrons enzymatically towards an endogenous, soluble cytochrome of type c. the cytochrome c in electron acceptor function was purified to homogeneity and appeared as a single protein band in a dodecyl sulfate disc gel electrophoresis ...	1983	6309643
genetic and physical map of the structural genes (nifh,d,k) coding for the nitrogenase complex of rhodopseudomonas capsulata.	functional genes coding for the structural components of the nitrogenase complex (nifh,d,k) have been cloned on an 11.8-kilobase-pair hindiii fragment of dna from the photosynthetic bacterium rhodopseudomonas capsulata. the genes were physically mapped by hybridization of individual cloned nif genes from klebsiella pneumoniae and anabaena sp. strain 7120 to southern blots of hindiii digests of the cloned r. capsulata fragment, after introduction of hindiii sites into the latter at specified loca ...	1983	6311797