Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| branhamella catarrhalis cellulitis around a cerebrospinal fluid shunt: case report. | a cellulitis surrounding a cerebrospinal fluid shunt caused by branhamella catarrhalis is described. this is the first reported case of a cellulitis caused by this bacterium. | 1985 | 2865299 |
| somatostatin-like immunoreactivity (sli) in pancreatic and intestinal tissues of the duck. a possible origin for the portal circulating sli. | substances with somatostatin-like immunoreactivity (sli) were extracted using 2 n acetic acid, from the three pancreatic lobes and the intestine of the duck. the concentration of sli was found to be very high in the pancreas (4.2 micrograms/g wet weight), the splenic lobe containing 80% of pancreatic sli compared with 10% for the dorsal and 10% for the ventral lobes. sli was equally distributed between duodenum, jejunum and ileum and between their mucosal and muscular layers. chromatography of p ... | 1985 | 2867025 |
| human t-cell leukemia/lymphoma viruses: atl and aids. | 1985 | 2897327 | |
| serotonergic regulation of the brain and gut beta-endorphin and dynorphin content in the rat. | a specific radioimmunoassay was used to measure immunoreactive dynorphin (ir-dyn) and beta-endorphin (ir-be) in the brain, pituitary and gut, following a pharmacological manipulation of the serotonin system. administration of the serotonin receptor agonist m-chlorophenylpiperazine (m-cpp, 2.5-5 mg/kg ip) or the serotonin releasing agent fenfluramine (20-40 mg/kg ip) induced a significant increase in the hypothalamic ir-be content and a decrease in its anterior pituitary level. these effects were ... | 1985 | 2870487 |
| measuring gene expression with light. | light is produced by recombinant escherichia coli that contain lux genes cloned from the marine bacterium vibrio fischeri. the bioluminescence phenotype requires genes for regulatory and biochemical functions, the latter encoded by five lux genes contained in a single operon. these lux genes were disconnected from their native promoter and inserted into the transposon mini-mu. the resulting transposon, mini-mulux, could induce mutations by insertional inactivation of a target gene, and the lux d ... | 1985 | 2983423 |
| respiration-driven na+ pump and na+ circulation in vibrio parahaemolyticus. | sodium circulation in vibrio parahaemolyticus was investigated. we observed respiration-driven na+ extrusion from cells by using a na+ electrode. the na+ extrusion was insensitive to a proton conductor, carbonyl cyanide m-chlorophenylhydrazone, and sensitive to a respiratory inhibitor, cn-. these results support the idea of the existence of a respiratory na+ pump in v. parahaemolyticus. the respiration-driven na+ extrusion was observed only under alkaline conditions. | 1985 | 2985548 |
| respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mrna, identification of cleavage activation site and amino acid sequence of n-terminus of f1 subunit. | the amino acid sequence of respiratory syncytial virus fusion protein (fo) was deduced from the sequence of a partial cdna clone of mrna and from the 5' mrna sequence obtained by primer extension and dideoxysequencing. the encoded protein of 574 amino acids is extremely hydrophobic and has a molecular weight of 63371 daltons. the site of proteolytic cleavage within this protein was accurately mapped by determining a partial amino acid sequence of the n-terminus of the larger subunit (f1) purifie ... | 1985 | 2987829 |
| conditioned taste aversion suppresses induction of delayed-type hypersensitivity immune reactions. | conditioned taste aversion was induced in mice by pairing saccharin drinking with an intraperitoneal injection of lithium chloride, a toxic but nonimmunosuppressive drug. conditioned mice showed not only suppressed saccharin drinking but also a 75% reduction in the induction of delayed-type hypersensitivity immune responses to low doses of sheep erythrocytes. this effect was observed with doses of lithium chloride which had no effect of their own on immune functions. in addition, a reduction in ... | 1985 | 2987987 |
| receptors for epidermal growth factor in the rat uterus. | uterine membranes contain high affinity [dissociation constant (kd) = 0.36 nm], saturable binding sites for [125i]iodo-epidermal growth factor (egf). the binding of [125i] iodo-egf is specific since it is abolished by excess unlabeled egf but not by excess unlabeled insulin, fibroblast growth factor, or multiplication-stimulating activity. incubation of [125i] iodo-egf with uterine membranes, followed by chemical cross-linking with disuccinimidyl suberate and detergent extraction reveals a major ... | 1985 | 2988913 |
| opiate- and alpha 2-adrenoceptor-induced hyperpolarizations of locus ceruleus neurons in brain slices: reversal by cyclic adenosine 3':5'-monophosphate analogues. | the purpose of this study was to investigate the ionic and second messenger mechanisms underlying the hyperpolarizations induced by the selective alpha 2-adrenoceptor agonist clonidine and the opiate agonist morphine in the locus ceruleus. intracellular recordings were carried out in rat brain slices, and drugs at known concentrations were administered in the perfusate. the cyclic adenosine 3':5'-monophosphate (camp) analogues 8-bromo-camp and dibutyryl camp, while not altering basal activity, r ... | 1985 | 2993542 |
| a nonsteroid anti-inflammatory drug exacerbates coxsackie b3 murine myocarditis. | nonsteroid anti-inflammatory drugs are often used to treat myalgias and arthralgias in enteroviral infections, but their effects on acute viral myocarditis are unknown. the effect of the nonsteroidal anti-inflammatory drug, ibuprofen, on acute viral myocarditis was studied in 75 four week old male balb/c mice infected with 1.75 x 10(7) plaque-forming units of coxsackie virus b3 on day 0. ibuprofen was given intraperitoneally at a dose of 15 mg/kg body weight daily. the mice were assigned to four ... | 1985 | 2995470 |
| the biologic significance of the mixed lymphocyte kidney culture in humans. | the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ... | 1985 | 2996186 |
| effect of pyrimidinone treatment on lethal and immunosuppressive murine cytomegalovirus infection. | the 2-amino-5-halo-pyrimidinones, which are potent interferon inducers and antiviral agents, were found to be protective against lethal cytomegalovirus (cmv) challenge in weanling or neonatal mice when administered before virus challenge. this protection was dependent upon the dosage of pyrimidinone administered. weanling mice infected with a sublethal challenge of cmv exhibited moderate to severe immunosuppression as measured by reduced splenic cell blastogenic responses in vitro to the mitogen ... | 1985 | 3000290 |
| purification of a metalloproteinase inhibitor from human rheumatoid synovial fluid. | a metalloproteinase inhibitor present in human rheumatoid synovial fluid was purified by a combination of heparin-sepharose chromatography, concanavalin a-sepharose chromatography, ion-exchange chromatography and gel filtration. the mr of the purified inhibitor was 28000 by sds/polyacrylamide-gel electrophoresis and 30000 by gel filtration. the inhibitor blocked the activity of the metalloproteinases collagenase, gelatinase and proteoglycanase, but not thermolysin or bacterial collagenase. the s ... | 1985 | 3000352 |
| [effects of 5 derivatives of 3-azabicyclo [3,3,1]nonanes on isolated guinea pig ileum myenteric plexus-longitudinal muscle]. | 1985 | 3020868 | |
| non-immune igg f(ab')2 binding to group c and g streptococci is mediated by structures on gamma chains. | the present investigation was designed to determine whether the heavy or the light immunoglobulin chain is involved in the non-immune binding of igg f(ab')2 fragments to specific surface receptors on human group c and g streptococci. purified human polyclonal igg was mildly reduced with dithiothreitol and alkylated with iodoacetamide. light (l) and heavy (h) chains were separated. intact igg and purified l and h chains of polyclonal immunoglobulin g were tested in an inhibition assay for non-imm ... | 1985 | 3156400 |
| direct selection of mutations reducing transcription or translation of the reca gene of escherichia coli with a reca-lacz protein fusion. | when a reca-lacz protein fusion was cloned into phage lambda, the resulting transducing phage grew normally on wild-type escherichia coli, but its growth was severely inhibited in lexa(def) mutant strains that express reca constitutively at high levels. mutants of the transducing phage that grew on the lexa(def) strains were isolated and were found to affect production of the reca-beta-galactosidase hybrid protein. most mutants, including a number of nonsense mutants, were phenotypically lacz-. ... | 1985 | 3160689 |
| [neonatal heart rate variability]. | 1985 | 261135 | |
| transfection of genes for cell surface products involved in antigen presentation--applications to the understanding of autoimmunity. | 1985 | 2129493 | |
| hypertension in the immediate postoperative period. | sixty patients out of 1,844 recovery room admissions had significant postoperative arterial hypertension. nearly 60% of them had a history of hypertension. the postoperative hypertension usually began within 30 min from the end of operation and lasted about 2 hours. in 20% of the patients it lasted 3 hours or longer. complications attributable to hypertension were confined to this latter group. the principal factors possibly contributing to the pressure elevations were pain (35%), hypercarbia (1 ... | 1985 | 238547 |
| inhibition of transcription by dna binding proteins of sarcoma-180 ascites cells. | 1985 | 218884 | |
| [a challenge: the profession and the dental students]. | 1985 | 268548 | |
| preparation areas resisting displacement of artificial crowns. | the areas of luting cement resisting rotation of artificial full crowns subjected to non-axial forces were calculated for varying values of preparation diameter, height, and convergence angle. the calculations were performed for regular conical and pyramidal preparations. the results showed that the resisting areas are larger in pyramidal than in conical preparations. increasing the convergence angle resulted in an almost linear reduction in resisting area. the reduction was particularly pronoun ... | 1985 | 268415 |
| [present situation and outlook of the campaign for abolition of practical nurse system--asking some questions of omori, president of the japanese nursing association]. | 1985 | 261186 | |
| the ada conference on increasing access to dental care. a report. | 1985 | 268525 | |
| [prognostic indices of acute lymphoblastic leukemia in the child. proposal for a prognostic classification and case contribution]. | 1985 | 271763 | |
| different effects of the ionophore a-23187 and d-glucose on 45ca2+ fluxes in isolated islets of ob/ob-mice. | fluxes of 45ca2+ were studied in beta-cell rich islets of non-inbred ob/ob-mice, using lacl3 to wash out extra-cellular and superficially bound 45ca2+. the ionophore a-23187 (10 microm) increased the 45ca2+ uptake in islets both at 3 and 20 mm d-glucose, the effect being more pronounced after 10 min than after 120 min of incubation. in incubations for 120 min, 20 mm d-glucose induced a higher uptake of 45ca2+ than did a-23187. the ionophore enhanced the unidirectional efflux of 45ca2+ from prelo ... | 1986 | 362823 |
| [the role of physiotherapy in recovery of patients with traumatic disorders of the peripheral nervous system]. | 1986 | 212863 | |
| the average rcbf pattern of resting consciousness studied with a new colour display system. | 1986 | 268800 | |
| eicosapentaenoic acid inhibits synthesis and secretion of triacylglycerols by cultured rat hepatocytes. | primary cultures of rat hepatocytes were used to study the effects of eicosapentaenoic and oleic acid on synthesis and secretion of triacylglycerols associated with very low density lipoproteins. from the experiments the following was observed. oleic acid markedly stimulates secretion as well as synthesis of triacylglycerols, whereas eicosapentaenoic acid causes very little or no increase in secretion or synthesis as compared to a fatty-acid-free medium. the effects could already be observed aft ... | 1986 | 3021226 |
| eicosapentaenoic acid inhibits synthesis and secretion of triacylglycerols by cultured rat hepatocytes. | primary cultures of rat hepatocytes were used to study the effects of eicosapentaenoic and oleic acid on synthesis and secretion of triacylglycerols associated with very low density lipoproteins. from the experiments the following was observed. oleic acid markedly stimulates secretion as well as synthesis of triacylglycerols, whereas eicosapentaenoic acid causes very little or no increase in secretion or synthesis as compared to a fatty-acid-free medium. the effects could already be observed aft ... | 1986 | 3021226 |
| spermine antagonises the effects of dexamethasone, glucagon and cyclic amp in increasing the activity of phosphatidate phosphohydrolase in isolated rat hepatocytes. | rat hepatocytes were incubated in monolayer culture, under serum free conditions, for 8 h. glucagon (10 nm), 8-(4-chlorophenylthio)adenosine 3',5'-cyclic monophosphate (100 microm) and dexamethasone (100 nm) increased the activity of phosphatidate phosphohydrolase by approx. 2-, 3.6- and 3.3-fold, respectively. spermine alone had no significant effect. spermine (2.5 mm) almost completely inhibited the glucagon induced increase in phosphohydrolase activity. it only partially inhibited the dexamet ... | 1986 | 3021529 |
| thymidine kinase with altered substrate specificity of acyclovir resistant varicella-zoster virus. | the acyclovir resistant mutant of varicella-zoster virus acv-r (a 8) induced the same level of thymidine kinase activity in infected cells as the parent kawaguchi strain. however, it induced less deoxycytidine kinase activity and did not induce phosphorylating activity for the nucleotide analogue, 9-(2 hydroxy-ethoxymethyl)-guanine-(acyclovir). another acyclovir resistant mutant, acv-r (a 4), which is cross-resistant to phosphonoacetate and is thought to be a viral dna polymerase mutant, induced ... | 1986 | 3022711 |
| identification and characterization of mg2+-dependent phosphotyrosyl protein phosphatase from rat liver cytosol. | although highly purified preparations of mg2+-dependent phosphoseryl protein phosphatase (also designated phosphatase ia or phosphatase 2c) dephosphorylated phosphotyrosyl histone, the activity has been resolved from phosphatase ia by polyacrylamide gel electrophoresis at ph 9.5. this novel phosphotyrosyl-specific protein phosphatase absolutely requires mg2+ or mn2+ for activity, is inhibited by zn2+, vanadate and fluoride, and has an optimal ph of 9.0 and mr = 50,000. certain properties of this ... | 1986 | 3022716 |
| isolation of the rhodopseudomonas sphaeroides form i ribulose 1,5-bisphosphate carboxylase/oxygenase large and small subunit genes and expression of the active hexadecameric enzyme in escherichia coli. | a library of cloned rhodopseudomonas sphaeroides dna was screened by colony hybridization for form i ribulose 1,5-bisphosphate carboxylase/oxygenase (rubpc/o) sequences using heterologous rubpc/o probes. a recombinant plasmid was identified that hybridized to both the anacystis nidulans and the r. sphaeroides form ii rubpc/o genes. subcloning of a hybridizing 4-kb smai fragment allowed expression of active enzyme in escherichia coli that was identical to form i rubpc/o based on polyacrylamide ge ... | 1986 | 3023189 |
| isolation of the rhodopseudomonas sphaeroides form i ribulose 1,5-bisphosphate carboxylase/oxygenase large and small subunit genes and expression of the active hexadecameric enzyme in escherichia coli. | a library of cloned rhodopseudomonas sphaeroides dna was screened by colony hybridization for form i ribulose 1,5-bisphosphate carboxylase/oxygenase (rubpc/o) sequences using heterologous rubpc/o probes. a recombinant plasmid was identified that hybridized to both the anacystis nidulans and the r. sphaeroides form ii rubpc/o genes. subcloning of a hybridizing 4-kb smai fragment allowed expression of active enzyme in escherichia coli that was identical to form i rubpc/o based on polyacrylamide ge ... | 1986 | 3023189 |
| primary biliary cirrhosis: assessment of the quantitative importance of the adenine nucleotide translocator protein as a major mitochondrial antigen. | the adenine nucleotide translocator protein (ant) is the first well-characterized mitochondrial polypeptide to be identified as an antigen for antimitochondrial autoantibodies (ama) in pbc sera. because of the potential use for a highly purified antigen as a tool in studying the aetiology of pbc, we have undertaken an assessment of the quantitative importance of ant as a pbc-specific mitochondrial antigen. immunoblotting and elisa techniques were used. both methods reveal pbc antibodies against ... | 1986 | 3028680 |
| primary biliary cirrhosis: assessment of the quantitative importance of the adenine nucleotide translocator protein as a major mitochondrial antigen. | the adenine nucleotide translocator protein (ant) is the first well-characterized mitochondrial polypeptide to be identified as an antigen for antimitochondrial autoantibodies (ama) in pbc sera. because of the potential use for a highly purified antigen as a tool in studying the aetiology of pbc, we have undertaken an assessment of the quantitative importance of ant as a pbc-specific mitochondrial antigen. immunoblotting and elisa techniques were used. both methods reveal pbc antibodies against ... | 1986 | 3028680 |
| hematologic care of the surgical patient. | 1986 | 3081528 | |
| hematologic care of the surgical patient. | 1986 | 3081528 | |
| second-generation cephalosporins. | 1986 | 3081544 | |
| [enterotoxin-like factor(s) produced by vibrio parahaemolyticus]. | culture filtrates obtained from several isolates of vibrio parahaemolyticus were tested for their enterotoxin-like activities. also their antigenic relationship with cholera enterotoxin and kanagawa hemolysin were tested. in the chinese hamster ovary (cho) cells assay which are usually employed for detecting cholera toxin or heat labile enterotoxin produced by enterotoxigenic escherichia coli, crude culture filtrates obtained from both kanagawa positive and negative strains isolated from patient ... | 1986 | 3084368 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |
| effect of chemical sympathectomy with oxidopamine on systemic blood flow in rats with acute pancreatitis. | in response to previous demonstrations that either surgical or chemical sympathectomy can ameliorate the severity of acute experimental pancreatitis, and suggestions that the benefits of sympathectomy are related to alterations in the absolute blood flow or the pattern of blood flow within the pancreas or splanchnic organs, we studied blood flow in rats with acute deoxycholate pancreatitis. the animals had preliminary chemical sympathectomy with a false neurotransmitter (oxidopamine) prior to th ... | 1986 | 3087651 |
| ontogenetic development of pancreatic thyrotropin-releasing hormone in human foetuses and in infants. | the ontogeny of thyrotropin-releasing hormone (trh) in pancreata of human foetuses from 15-36 weeks of gestation and of infants has been studied. trh was detectable in the pancreas of a 15 week old foetus; a progressive increase of pancreatic trh content was observed until the 34th week of gestation, whereas a progressive decrease was found in the late period of pregnancy and in 1 year old infants. in contrast, the pancreatic insulin content showed a progressive increase during the entire pregna ... | 1986 | 3092527 |
| effect of aldose reductase inhibitor (sorbinil) on integration of polyol pathway, pentose phosphate pathway, and glycolytic route in diabetic rat lens. | this study examines the effect of an aldose reductase inhibitor (sorbinil) on the flux of specifically labeled glucose through alternative pathways of metabolism in the lens of normal and diabetic rats 1 wk after the induction of diabetes with alloxan. in the diabetic rat lens, there was an apparent increase in the flux of glucose through the pentose phosphate pathway (ppp), as measured by the difference in the yields of 14co2 from [1-14c]glucose and [6-14c]glucose [c1-c6], this value was 0.087 ... | 1986 | 3093302 |
| purification and partial characterization of a vibrio hollisae hemolysin that relates to the thermostable direct hemolysin of vibrio parahaemolyticus. | hemolysin produced by vibrio hollisae (vh-rtdh), which is related to the thermostable direct hemolysin (vp-tdh) of vibrio parahaemolyticus, was studied. vh-rtdh was purified by successive column chromatographies on diethylaminoethyl-cellulose and an immunoaffinity column coupled with anti vp-tdh immunoglobulin. the purified toxin was homogeneous, as demonstrated by conventional and sodium dodecyl sulfate--polyacrylamide gel electrophoresis (page). the molecular weight of vh-rtdh was slightly sma ... | 1986 | 3094925 |
| intracellular distribution of 73,000 and 72,000 dalton heat shock proteins in hela cells. | intracellular localization of 73,000 and 72,000 dalton heat shock proteins (hsp73/72) in hela cells that were heat shocked or treated with chemical stressors was investigated using indirect immunofluorescent staining. the antiserum used specifically recognized the hsp73/72 in hela cells, and hsps were increased by heating cells at 42 degrees c for 2 or 4 h and by prior treatment with chemical stressors (sodium arsenite, cadmium chloride, 8-hydroxyquinoline and ethanol). there was diffuse cytopla ... | 1986 | 3098874 |
| induction of phage-like particles from a pathogenic strain of vibrio parahaemolyticus by mitomycin c. | a rapid drop of absorbance of cell suspensions due to cell lysis was observed midway in cell growth of a pathogenic strain, vibrio parahaemolyticus wp1, when the cells were incubated in a growth medium containing mitomycin c. we succeeded in isolating phage-like particles from the cell lysate, but not from that of the non-pathogenic strain, wp28. the purified particle has a clear-cut envelope and is hexagonal in shape with no tail. the size of particles is about 65 millimicrons on the diagonal a ... | 1986 | 3101681 |
| nucleotide sequence and in vivo expression of the ilvy and ilvc genes in escherichia coli k12. transcription from divergent overlapping promoters. | the ilvc gene of escherichia coli k12 encodes acetohydroxy acid isomeroreductase, the second enzyme in the parallel isoleucine-valine biosynthetic pathway. previous data have shown that transcription of the ilvc gene is induced by the acetohydroxy acid isomeroreductase substrates, acetohydroxybutyrate or acetolactate, and that this substrate induction of ilvc expression is mediated by a positive activator encoded by the ilvy gene. we report here the isolation and complete nucleotide sequence of ... | 1986 | 3003115 |
| effect of the hyp mutation and diet-induced hyperparathyroidism on renal parathyroid hormone- and forskolin-stimulated adenosine 3',5'-monophosphate production and brush border membrane phosphate transport. | the present study was undertaken to investigate the effect of the hyp mutation and diet-induced hyperparathyroidism on renal responsiveness to pth and forskolin and to determine whether the renal brush border membrane phosphate transport defect is expressed in the vitamin d- and calcium-deficient hyp mouse. our results indicate that pth is a potent activator of camp synthesis in renal slices obtained from vitamin d-replete normal mice and hyp littermates. however, in the mutants, the amount of c ... | 1986 | 3004890 |
| cloning and expression of the exfoliative toxin b gene from staphylococcus aureus. | exfoliative toxin type b is produced by bacteriophage group ii strains of staphylococcus aureus and is a causative agent of staphylococcal scalded-skin syndrome. in addition to exfoliative toxin b, most isolates also produce a bacteriocin and are immune to the action of the bacteriocin. these phenotypes, as well as resistance to cadmium, were lost after elimination of a 37.5-kilobase plasmid, prw001, from s. aureus ut0007. transduction and transformation showed that prw001 carries the structural ... | 1986 | 3009410 |
| a novel type of human papillomavirus associated with genital neoplasias. | the role of human papillomaviruses (hpvs) in the development of genital neoplasias has been well documented. the genomes of two hpv types, hpv16 and hpv18, have been found to be associated with about 70% of invasive carcinomas of the uterine cervix. as, under non-stringent hybridization conditions, hpv dna sequences have been detected in about 90% of cervical carcinomas, it seems likely that additional hpv types are associated with these tumours. here we report the molecular cloning and characte ... | 1986 | 3012352 |
| transposable resistance to trimethoprim and 0/129 in vibrio cholerae. | vibrio cholerae biotype el tor strain bm2508, resistant to trimethoprim, 0/129, streptomycin and spectinomycin was isolated from the faeces of a child with severe diarrhoea. resistance to trimethoprim and 0/129 was due to a dihydrofolate reductase type i and resistance to streptomycin-spectinomycin to a 3'',9-aminoglycoside-aminocyclitol adenylyltransferase. the resistance genes were not transferable to escherichia coli and, as inferred from ultracentrifugation in cesium chloride-ethidium bromid ... | 1986 | 3013828 |
| regulation of lateral flagella gene transcription in vibrio parahaemolyticus. | two distinctly different organelles of locomotion are produced by vibrio parahaemolyticus. the polar flagellum is responsible for motility in a liquid environment (swimming), and the lateral flagella enable the bacteria to move over surfaces (swarming). synthesis of lateral flagella occurs when v. parahaemolyticus is grown on agar media but not when it is grown in liquid media. we used lux (luminescence gene) fusions to conveniently and sensitively analyze the factors which influence transcripti ... | 1986 | 3013835 |
| [experimental nephritis induced by coxsackie b4 virus in mice--transient mesangial proliferation associated with acute viremia]. | 1986 | 3018334 | |
| induction of an unusual type of shared phosphorylation in human and avian cells by tumor-promoting phorbol esters or transformation. | alterations in patterns of protein phosphorylation after exposure to phorbol esters were compared in chicken embryo fibroblasts and kd cells (a human fibroblast line) by two-dimensional gel electrophoresis. a substantial increase in phosphorylation was observed of a major, markedly acidic protein of pi = 4.5 in two-dimensional gels of each cell type. however, the apparent molecular weights of these phosphoproteins differed substantially in the two species with a molecular weight of 67,000 in chi ... | 1986 | 3019525 |
| evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes. | two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ... | 1986 | 3020551 |
| evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes. | two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ... | 1986 | 3020551 |
| response of young pigs to foot-and-mouth disease oil emulsion vaccination in the presence and absence of maternally derived neutralising antibodies. | serum samples and bodyweights were taken at regular intervals for six to seven months from piglets, born to foot-and-mouth disease (fmd) vaccinated or unvaccinated sows, which had been vaccinated at one, two, four or eight weeks old. young pigs, devoid of maternally derived antibodies (mda), were capable of responding to fmd vaccination at one week old, with no deleterious effects on their growth rate. however, their immunity to experimental infection at six to seven months old was poor (33.3 pe ... | 1986 | 3020657 |
| potent antagonism of escherichia coli, bacteroides ovatus, fusobacterium varium, and enterococcus faecalis, alone or in combination, for enteropathogens in anaerobic continuous flow cultures. | interactions between representative strains of four predominant resident bacteria of the human colon, escherichia coli, enterococcus faecalis, bacteroides ovatus, and fusobacterium varium, and strains of seven enteropathogens, yersinia enterocolitica, shigella flexneri, salmonella typhimurium, vibrio parahaemolyticus, v. cholerae serogroup non o1, staphylococcus aureus, and clostridium perfringens, were examined in studies with an anaerobic continuous flow culture system and medium resembling th ... | 1986 | 2875188 |
| [enteropathogens in travellers returning from the tropics]. | diarrhea is not only the most common health hazard during travel in the tropics but also the most frequent condition which prompts returning travellers to see a physician. the prevalence of bacterial and parasitic enteropathogens in people attending our outpatient department after returning from the tropics has been studied and the laboratory results of stool examinations have been compared with clinical symptoms. of the 173 persons enrolled, 19 (11%) harboured bacterial pathogens and pathogenic ... | 1986 | 2875520 |
| report of a wound infection caused by vibrio parahaemolyticus and vibrio vulnificus. | the present case describes a foot wound caused by a clam shell from which both vibrio parahaemolyticus and vibrio vulnificus were recovered. although extraintestinal infections associated with vibrio parahaemolyticus have been reported previously, the simultaneous isolation of two marine vibrios from our case suggests that these organisms may coexist in mixed infections from a common source. | 1986 | 2875824 |
| a dot immunobinding assay (dia) on nitrocellulose membrane for the serological detection of antibodies to entamoeba histolytica. | a rapid, cheap, simple and specific serological test of adequate sensitivity for detecting igg antibodies against entamoeba histolytica antigen is described. axenically cultured amoebic antigen was used to precoat the nitrocellulose membrane. the strips were incubated with test samples and later with horseradish peroxidase (hrpo) labelled protein-a conjugate. a dark blue spot was obtained by treatment with peroxidase substrate, 4-chloro-1-naphthol, in positive samples. serum samples from 32 heal ... | 1986 | 2885949 |
| differential depression of hypoglossal nerve activity by alcohol. protection by pretreatment with medroxyprogesterone acetate. | upper airway obstruction during sleep occurs more commonly in men than in women and has been treated with progestational agents with some success. alcohol ingestion exacerbates sleep apnea, and recent studies have established that alcohol depresses the respiratory motor activity to upper airway muscles more than that to the diaphragm, a response pattern that favors upper airway obstruction during inspiration. to investigate the possibility that progesterone provides some protection from this act ... | 1986 | 2935056 |
| [fertilization results in superovulated ewes and goats after transmural-intrauterine insemination controlled by laparoscopy]. | a comparative experiment in oestrous synchronized and superovulated ewes and does is described on which fertilization was studied following natural mating or transmural intrauterine insemination by laparoscopy. thirty-two ewes were inseminated into each uterine horn with 50-100 microliter of fresh undiluted semen, whereas 26 ewes were mated by fertile rams. fertilization rates of eggs recovered 5-7 days later were 93.7% (163/174) and 60.5% (78/129) respectively. eight does were inseminated by la ... | 1986 | 2940732 |
| possible role of helper and cytolytic t cells in mycobacterial infections. | 1986 | 2952852 | |
| the source of malonyl-coa in rat heart. the calcium paradox releases acetyl-coa carboxylase and not propionyl-coa carboxylase. | the formation of malonyl-coa in rat heart is catalyzed by cytosolic acetyl-coa carboxylase. the existence of this enzyme in heart is difficult to prove by the abundant occurrence of mitochondrial propionyl-coa carboxylase, which is also able to catalyze the carboxylation of acetyl-coa. we used the calcium paradox as a tool to separate cytosolic components from the remaining heart, and found that acetyl-coa carboxylase activity was preferentially released, like lactate dehydrogenase and carnitine ... | 1986 | 2869975 |
| serotonin and 5-hydroxyindoleacetic acid in brains of suicide victims. comparison in chronic schizophrenic patients with suicide as cause of death. | serotonin (5-ht) and 5-hydroxyindoleacetic acid concentrations were determined in various brain areas of nonschizophrenic suicide victims, chronic schizophrenic patients with or without suicide as the cause of death, and normal control subjects without psychiatric or neurologic disorders. serotonin concentrations in the basal ganglia were significantly elevated in suicide victims and chronic schizophrenic patients, as were 5-hydroxyindoleacetic acid concentrations in the occipital cortex. these ... | 1986 | 2423050 |
| growth of human mammary carcinoma cells from biopsy specimens in serum-free medium on extracellular matrix. | a method developed for the initiation and maintenance in primary culture of human normal mammary epithelial cells was adopted for the growth of epithelial cells from 45 primary human breast tumors. the cells were grown on a naturally produced extracellular matrix (ecm) or on regular tissue culture plastic in a serum-free medium containing growth supplements and high-density lipoprotein (hdl). successful enzymatic dissociation of the tumor biopsy into organoid structures and cell aggregates was c ... | 1986 | 2427456 |
| fate of n-(2-hydroxypropyl)methacrylamide copolymers with pendent galactosamine residues after intravenous administration to rats. | n-(2-hydroxypropyl)methacrylamide (hpma) copolymers bearing galactosamine residues accumulate in the liver after intravenous administration to rats (duncan, r., kopecek, j., rejmanová, p. and lloyd, j.b. (1983) biochim. biophys. acta 755, 518-521). in this study hpma copolymers bearing pendent galactosamine residues (1.0-11.6 mol%) were injected intravenously into rats and their rates of blood clearance and liver accumulation were measured. a level of substitution of 4 mol% was found to be suffi ... | 1986 | 3942780 |
| distribution of gamma-carboxyglutamic acid residues in partially carboxylated human prothrombins. | the role of gamma-carboxyglutamic acid in prothrombin has been examined using partially carboxylated variant prothrombins isolated from a person with a hereditary defect in vitamin k-dependent carboxylation. these species differ in gamma-carboxyglutamic acid content, distribution, and function, as monitored by metal binding properties, conformational transitions, phospholipid binding, and calcium-dependent coagulant activity (borowski, m., furie, b. c., goldsmith, g. h., and furie, b. (1985) j. ... | 1986 | 3944102 |
| distribution of gamma-carboxyglutamic acid residues in partially carboxylated human prothrombins. | the role of gamma-carboxyglutamic acid in prothrombin has been examined using partially carboxylated variant prothrombins isolated from a person with a hereditary defect in vitamin k-dependent carboxylation. these species differ in gamma-carboxyglutamic acid content, distribution, and function, as monitored by metal binding properties, conformational transitions, phospholipid binding, and calcium-dependent coagulant activity (borowski, m., furie, b. c., goldsmith, g. h., and furie, b. (1985) j. ... | 1986 | 3944102 |
| purification and partial characterization of a non-o1 vibrio cholerae hemolysin that cross-reacts with thermostable direct hemolysin of vibrio parahaemolyticus. | a newly identified hemolysin (nag-rtdh), which is related to the thermostable direct hemolysin (vp-tdh) of vibrio parahaemolyticus produced by non-o1 vibrio cholerae, was studied. nag-rtdh was purified by successive column chromatographies on deae-cellulose and an immunoaffinity column coupled with anti-vp-tdh immunoglobulin. the molecular weight of nag-rtdh was estimated as 18,500, similar to that of vp-tdh, as judged by sodium dodecyl sulfate slab gel electrophoresis, but its charge or molecul ... | 1986 | 3957429 |
| interaction of histidine-rich glycoprotein with fibrinogen and fibrin. | histidine-rich glycoprotein (hrgp) is a human plasma and platelet protein of apparently diverse biological functions. in this study a new interaction for hrgp is described. hrgp specifically interacts with fibrinogen as demonstrated by two independent systems. using an enzyme-linked immunosorbent assay it was demonstrated that hrgp bound to adsorbed fibrinogen in a concentration-dependent and saturable manner, with an apparent dissociation constant (kd) of 6.7 nm. the binding was specific, rever ... | 1986 | 3958188 |
| influence of isoflurane on hypoxic pulmonary vasoconstriction in dogs. | the authors studied the influence of locally administered isoflurane anesthesia on the pulmonary vascular response to regional alveolar hypoxia (hypoxic pulmonary vasoconstriction [hpv]) over a range of cardiac outputs (cos) in seven mechanically ventilated, closed-chest dogs. the right lung was ventilated with 100% o2 throughout the study. the left lung was ventilated with either 100% o2 (normoxia) or an hypoxic gas mixture (hypoxia). different alveolar concentrations of isoflurane (0, 1, and 2 ... | 1986 | 3963450 |
| decontamination of cell lines by selective elimination of mycoplasmas. | 1986 | 3964004 | |
| decontamination of cell lines by selective elimination of mycoplasmas. | 1986 | 3964004 | |
| purification of the thermostable direct hemolysin of vibrio parahaemolyticus by immunoaffinity column chromatography. | a simple method involving immunoaffinity column chromatography to purify the thermostable direct hemolysin of vibrio parahaemolyticus was developed. the thermostable direct hemolysin purified from the culture supernatant of a strain isolated from the first reported case of v. parahaemolyticus infection in china in 1985 was indistinguishable from the hemolysins purified from strains isolated in japan. | 1986 | 3697844 |
| evidence for the presence of a novel biosynthetic pathway for norspermidine in vibrio. | enzymatic studies of the cell extracts of vibrio alginolyticus and v. parahaemolyticus provided evidence that there exists a novel biosynthetic pathway for norspermidine (nh2(ch2)3nh(ch2)3nh2), a major polyamine species. in this pathway, the schiff base formed between aspartic beta-semialdehyde and 1,3-diaminopropane is first reduced by a nadph-dependent enzyme to yield "carboxynorspermidine" (nh2(ch2)3nh(ch2)2ch(nh2)cooh), which is in turn decarboxylated by a pyridoxal phosphate dependent enzym ... | 1986 | 3697846 |
| [cytogenetic activity of metabolites of pesticides representing different classes of chemical compounds]. | the cytogenetic activity of some substances formed in agricultural plants during metabolism of pesticides of four classes of chemical compounds was studied in the culture of human peripheric blood lymphocytes. metabolites were shown either to have mutagenic properties similar to those of the initial compounds (ziramtetramethylthiourea, both being mutagens; captan-phthalimide, both possessing no cytogenetic activity) or to be considerably transformed in comparison with them as a result of deactiv ... | 1986 | 3705167 |
| cancer chemotherapy. | 1986 | 3705387 | |
| hereditary cerebral amyloid angiopathy: the amyloid fibrils contain a protein which is a variant of cystatin c, an inhibitor of lysosomal cysteine proteases. | hereditary cerebral hemorrhage with amyloidosis is an autosomal dominant form of amyloidosis restricted to the cerebral vasculature. we have previously demonstrated that the amyloid protein subunit is similar to cystatin c (or gamma-trace), an inhibitor of lysosomal cysteine proteinases, and homologous to kininogens. high pressure liquid chromatography tryptic fingerprint analysis was developed to distinguish cystatin c from the amyloid protein. moreover, we isolated and sequenced tryptic peptid ... | 1986 | 3707586 |
| brain macrophages in human cortical contusions as indicator of survival period. | the aim of this study was to establish a morphologic time scheme with which cases of cerebral contusion with unknown survival periods can be dated. our study of 275 cases was limited to qualitative and quantitative changes in macrophages. the appearance of macrophages and their distribution as well as their content of neutral fat, esterified cholesterol, erythrocytes, siderin, hematoidin, and ceroid were correlated with the survival period. for each cytologic criterium, the observation period, d ... | 1986 | 3710334 |
| synthetic oligodeoxyribonucleotide probes to detect kanagawa phenomenon-positive vibrio parahaemolyticus. | synthetic oligodeoxyribonucleotide probes were used in the colony hybridization test to examine the association between the kanagawa phenomenon (kp) and the thermostable direct hemolysin gene (tdh) of vibrio parahaemolyticus. representative v. parahaemolyticus strains with a variety of kp reactions and 17 other vibrio species were examined for homology with four synthetic oligodeoxyribonucleotide probes (19 to 21 bases long) representing different regions of the tdh structural gene. under string ... | 1986 | 3711299 |
| low molecular weight immunosuppressive factors found in elevated amounts in cancer ascitic fluids of mice. 1. isolation, identification and immunosuppressive effects of uric acid and uracil. | a definite increase in two low molecular weight factors, g10-2 and g10-3 was found in ehrlich ascitic fluids, parallel to tumor growth. the isolation and identification of the two factors were attempted through gel filtration and reversed phase column chromatography, using ascitic fluids obtained 13 days after intraperitoneal implantation of ehrlich tumor cells. as a result, two highly purified factors were observed upon examination by high performance liquid chromatography. additional analytica ... | 1986 | 3711673 |
| preliminary analyses on persistence of vibrio parahaemolyticus in a brackish water clam, corbicula japonica. | 1986 | 3712889 | |
| [studies on the protein-binding of drugs with continuous ultrafiltration. 8. in vitro quantification of the interactions of phenprocoumon, warfarin and methylsulfinyl warfarin with phenylbutazone on human serum albumin]. | 1986 | 3718179 | |
| absence of role of endothelium in the response of isolated porcine coronary arteries to acetylcholine. | isolated precontracted arteries of various vascular beds relax in response to acetylcholine only if the endothelium is present. one explanation for this is that this drug stimulates the endothelial cells to release a vasodilator substance that in turn relaxes the underlying smooth muscle. to determine whether this mechanism is concerned also in the acetylcholine contraction of isolated porcine coronary arteries transverse strips of the extramural part of the left circumflex artery were used for ... | 1986 | 3719611 |
| [effect of alcohol on gastrointestinal absorption, distribution and kinetics of am-241 in the bodies of rats]. | 1986 | 3721221 | |
| seasonal distribution of facultatively enteropathogenic vibrios (vibrio cholerae, vibrio mimicus, vibrio parahaemolyticus) in the freshwater of the elbe river at hamburg. | between june 1981 and december 1982 the incidence of vibrio cholerae, v. mimicus and v. parahaemolyticus was determined at two sampling sites on the elbe river at hamburg. a total of 183 strains was isolated from 147 water samples. of these, 107 belonged to non-01 v. cholerae (ten strains producing a cholera-like enterotoxin); 33 were identified as v. mimicus, including two enterotoxin producers; 42 strains were kanagawa-negative cultures of v. parahaemolyticus; and one was v. fluvialis. the hig ... | 1986 | 3722029 |
| anticandidal activities of terconazole, a broad-spectrum antimycotic. | terconazole is a new triazole ketal derivative with broad-spectrum in vitro and in vivo antifungal activities. this study further characterizes the effects of terconazole in vitro on yeast cell growth, viability, and morphology. terconazole inhibited the growth of candida albicans atcc 44859 in a concentration-related manner, but with modest effects noted at levels from 10(-8) to 10(-5) m when the yeast was grown on media favoring the cell form. the inhibitory potency of terconazole on yeast cel ... | 1986 | 3729366 |
| survival of vibrio parahaemolyticus in cold smoked fish. | fresh samples of mullet (mugil cephalus) and oil sardines (sardinella longiceps) obtained from a fish market were subjected to cold smoking. some of the samples harboured low levels of vibrio parahaemolyticus. after cold smoking, however, many samples showed relatively high levels of v. parahaemolyticus suggesting that a small population of naturally occurring organisms could multiply to significant levels during the process of cold smoking or during subsequent storage at room temperature. never ... | 1986 | 3729375 |
| purification and initial characterization of the 71-kilodalton rat heat-shock protein and its cognate as fatty acid binding proteins. | the major rat heat-shock (stress) protein and its cognate were purified to electrophoretic homogeneity from livers of heat-shocked rats. both proteins exhibited similar behavior on a variety of column chromatography matrices but were separable by preparative isoelectric focusing under nondenaturing conditions by virtue of a 0.2 ph unit difference in isoelectric point. both purified proteins had similar physical properties, suggesting the possibility that they may have similar biological function ... | 1986 | 3730359 |
| [chemical properties of o-antigenic lipopolysaccharides isolated from vibrio parahaemolyticus o2 and o9, in particular, the separation and identification of 2-keto-3-deoxy-5-phosphoryl-octonate found in their strong acid-hydrolysates]. | 1986 | 3735671 | |
| [isolation of vibrio parahaemolyticus from corbina (cynoscion squamipennis) in the gulf of nicoya, costa rica]. | 1986 | 3738288 | |
| measles-associated diarrhoea in northeastern thailand. | a significant proportion of the illness and death of diarrhoeal diseases in the developing world is estimated to be due to the diarrhoea associated with measles. during february 1983-january 1984 a prospective study of measles in a hospital in northeastern thailand was conducted. a total of 550 cases of measles were studied. diarrhoea was the most frequent complication of measles, occurring in 233 cases (42.4%). the largest proportion (46.2%) of cases with diarrhoea occurred in may-july. childre ... | 1986 | 3738607 |
| effect of training on plasma anabolic and catabolic steroid hormones and their response during physical exercise. | 1986 | 3744643 | |
| [clinical aspects of acute intestinal infection caused by vibrio parahaemolyticus]. | 1986 | 3750950 |