Publications

expanding the paradigms of plant pathogen life history and evolution of parasitic fitness beyond agricultural boundaries.		2009	20041212
microbial diversity in a bagasse-based compost prepared for the production of agaricus brasiliensis.	edible mushrooms are renowned for their nutritional and medicinal properties and are thus of considerable commercial importance. mushroom production depends on the chemical composition of the basic substrates and additional supplements employed in the compost as well as on the method of composting. in order to minimise the cost of mushroom production, considerable interest has been shown in the use of agro-industrial residues in the preparation of alternative compost mixtures. however, the inter ...	2009	24031404
aerobic bacterial microflora of broad-snouted caiman (caiman latirostris) oral cavity and cloaca, originating from parque zoológico arruda câmara, paraíba, brazil.	the objective of this study was to isolate and identify the aerobic bacterial microflora from the oral cavity mucosa and cloaca's samples, collected from broad-snouted caiman (caiman latirostris), born and bred in captivity at parque zoológico arruda câmara, joão pessoa, paraíba, brazil. the most common bacteria were staphylococcus sp. (14.74%), corynebacterium sp. (13.68%), escherichia coli (13.68%) and shigella sp.(11.58%), and the less common were citrobacter sp. (1.05%), klebsiella pneumonia ...	2009	24031343
infectious complications associated with monoclonal antibodies and related small molecules.	biologics are increasingly becoming part of routine disease management. as more agents are developed, the challenge of keeping track of indications and side effects is growing. while biologics represent a milestone in targeted and specific therapy, they are not without drawbacks, and the judicious use of these "magic bullets" is essential if their full potential is to be realized. infectious complications in particular are not an uncommon side effect of therapy, whether as a direct consequence o ...	2009	19366915
pathogenic roles of the bacteria carried by bursaphelenchus mucronatus.	fifty strains of bacteria were isolated from six isolates of the nematode bursaphelenchus mucronatus (bm) from china and russia and identified using the biomerieux vitek 32 system. in bioassay, 3 bacterial strains showed the high levels of phytotoxin production while 19, 16, and 12 strains showed moderately, low and no phytotoxin production, respectively. inoculation of 2-month-old pinus thunbergii seedling with each of the six bm isolates showed that the mean number of days from inoculation to ...	2009	22661772
molecular analysis of endophytic bacteria from the genus bacillus isolated from tropical maize (zea mays l.).	endophytic bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. in the present study molecular methods were used for identifying bacillus endophytic bacteria isolated from brazilian sweet corn. sds-page of whole-cell protein extract of forty-two isolates revealed a high number of scrutinable bands. twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique ...	2009	24031395
on the inside.		2009	19802964
u(vi) sequestration in hydroxyapatite produced by microbial glycerol 3-phosphate metabolism.	previous studies have demonstrated the potential for removal of u(vi) from solution via precipitation of u(vi)-bearing calcium-phosphate (ca-p) minerals coupled to microbial hydrolysis of glycerol phosphate compounds. we evaluated this process in circumneutral-ph groundwater from area 2 of the u.s. department of energy field research center at oak ridge national laboratory. area 2 groundwater contains high concentrations of dissolved calcium (ca. 4 mm), and thus, release of phosphate during glyc ...	2009	19633115
ward round--crocodile bites in malawi: microbiology and surgical management.	we present a case series of 5 patients admitted over 5 months to queen elizabeth central hospital who had sustained injuries from a crocodile bite. three patients required amputation of a limb. the severe soft tissue injury associated with a crocodile bite and the unusual normal oral flora of the crocodile create challenges in treatment. progressive tissue destruction and haemolysis are complications of such infected wounds. an antibiotic regime is recommended that covers gram negative rods, ana ...	2009	19780476
diabetes does not affect outcome in patients with enterobacteriaceae bacteremia.	there is limited information about the effect of diabetes on the prognosis of patients with bacterial infections. we performed a retrospective cohort study to investigate possible correlations between diabetes and prognosis in patients with enterobacteriaceae bacteremia.	2009	19523241
potentials and limitations of microorganisms as renal failure biotherapeutics.	renal insufficiency leads to uremia, a complicated syndrome. it thus becomes vital to reduce waste metabolites and regulate water and electrolytes in kidney failure. the most common treatment of this disease is either dialysis or transplantation. although these treatments are very effective, they are extremely costly. recently artificial cells, microencapsulated live bacterial cells, and other cells have been studied to manage renal failure metabolic wastes. the procedure for microencapsulation ...	2009	19707412
contribution of biologically derived nanoparticles to disease.		2009	19767047
contribution of biologically derived nanoparticles to disease.		2009	19767047
metabolite sorting of a germplasm collection reveals the hydroxylase3 locus as a new target for maize provitamin a biofortification.	vitamin a deficiency, a global health burden, can be alleviated through provitamin a carotenoid biofortification of major crop staples such as maize (zea mays) and other grasses in the poaceae. if regulation of carotenoid biosynthesis was better understood, enhancement could be controlled by limiting beta-carotene hydroxylation to compounds with lower or no nonprovitamin a activity. natural maize genetic diversity enabled identification of hydroxylation genes associated with reduced endosperm pr ...	2009	19767386
purification and characterisation of an extracellular phytase from aspergillus niger 11t53a9.	an extracellular phytase from aspergillus niger 11t53a9 was purified about 51-fold to apparent homogeneity with a recovery of 20.3% referred to the phytase activity in the crude extract. purification was achieved by ammonium sulphate precipitation, ion chromataography and gel filtration. the purified enzyme behaved as a monomeric protein with a molecular mass of about 85 kda and exhibited maximal phytate-degrading activity at ph 5.0. optimum temperature for the degradation of phytate was 55°c. t ...	2009	24031427
back-translation for discovering distant protein homologies in the presence of frameshift mutations.	frameshift mutations in protein-coding dna sequences produce a drastic change in the resulting protein sequence, which prevents classic protein alignment methods from revealing the proteins' common origin. moreover, when a large number of substitutions are additionally involved in the divergence, the homology detection becomes difficult even at the dna level.	2010	20047662
diverse bacteria inhabit living hyphae of phylogenetically diverse fungal endophytes.	both the establishment and outcomes of plant-fungus symbioses can be influenced by abiotic factors, the interplay of fungal and plant genotypes, and additional microbes associated with fungal mycelia. recently bacterial endosymbionts were documented in soilborne glomeromycota and mucoromycotina and in at least one species each of mycorrhizal basidiomycota and ascomycota. here we show for the first time that phylogenetically diverse endohyphal bacteria occur in living hyphae of diverse foliar end ...	2010	20435775
characterization of plant carotenoid cyclases as members of the flavoprotein family functioning with no net redox change.	the later steps of carotenoid biosynthesis involve the formation of cyclic carotenoids. the reaction is catalyzed by lycopene beta-cyclase (lcy-b), which converts lycopene into beta-carotene, and by capsanthin-capsorubin synthase (ccs), which is mainly dedicated to the synthesis of kappa-cyclic carotenoids (capsanthin and capsorubin) but also has lcy-b activity. although the peptide sequences of plant lcy-bs and ccs contain a putative dinucleotide-binding motif, it is believed that these two car ...	2010	20460582
one-pot three-component synthesis of quinoxaline and phenazine ring systems using fischer carbene complexes.	one-pot three-component coupling of o-alkynylheteroaryl carbonyl derivatives with fischer carbene complexes and dienophiles leading to the synthesis of quinoxaline and phenazine ring systems has been investigated. this involves the generation of furo[3,4-b]pyrazine and furo[3,4-b]quinoxaline as transient intermediates, which were trapped with diels-alder dienophiles. this is the first report on furo[3,4-b]pyrazine intermediates.	2010	20625524
antioxidant enrichment and antimicrobial protection of fresh-cut fruits using their own byproducts: looking for integral exploitation.	fresh-cut fruit consumption is increasing due to the rising public demand for convenience and awareness of fresh-cut fruit's health benefits. the entire tissue of fruits and vegetables is rich in bioactive compounds, such as phenolic compounds, carotenoids, and vitamins. the fresh-cut fruit industry deals with the perishable character of its products and the large percentage of byproducts, such as peels, seeds, and unused flesh that are generated by different steps of the industrial process. in ...	2010	21535513
incidence of low-grade infection in aseptic loosening of total hip arthroplasty.	we investigated the hypothesis that many total hip arthroplasty revisions that are classified as aseptic are in fact low-grade infections missed with routine diagnostics.	2010	20919816
resistance to antibiotics in urinopathogenic bacteria isolated in patients attending kenyatta university health clinic, nairobi.	to determine the aetiological bacterial agents of urinary tract infections, within communities in kenyatta university, and current resistance levels to commonly available therapeutic agents.	2010	23057307
size does matter: application-driven approaches for soil metagenomics.	metagenomic analyses can provide extensive information on the structure, composition, and predicted gene functions of diverse environmental microbial assemblages. each environment presents its own unique challenges to metagenomic investigation and requires a specifically designed approach to accommodate physicochemical and biotic factors unique to each environment that can pose technical hurdles and/or bias the metagenomic analyses. in particular, soils harbor an exceptional diversity of prokary ...	2010	21076656
clinical effects and lethal and forensic aspects of propofol.	propofol is a potent intravenous anesthetic agent that rapidly induces sedation and unconsciousness. the potential for propofol dependency, recreational use, and abuse has only recently been recognized, and several cases of accidental overdose and suicide have emerged. in addition, the first documented case of murder using propofol was reported a few months ago, and a high profile case of suspected homicide with propofol is currently under investigation. a number of analytical methods have been ...	2010	20950316
clinical effects and lethal and forensic aspects of propofol.	propofol is a potent intravenous anesthetic agent that rapidly induces sedation and unconsciousness. the potential for propofol dependency, recreational use, and abuse has only recently been recognized, and several cases of accidental overdose and suicide have emerged. in addition, the first documented case of murder using propofol was reported a few months ago, and a high profile case of suspected homicide with propofol is currently under investigation. a number of analytical methods have been ...	2010	20950316
rest energy expenditure is decreased during the acute as compared to the recovery phase of sepsis in newborns.	little is known with respect to the metabolic response and the requirements of infected newborns. moreover, the nutritional needs and particularly the energy metabolism of newborns with sepsis are controversial matter. in this investigation we aimed to evaluate the rest energy expenditure (ree) of newborns with bacterial sepsis during the acute and the recovery phases.	2010	20653967
nasopharyngeal gram-negative bacilli colonization in brazilian children attending day-care centers.	this study aimed at determining prevalence and resistance profile of gram-negative bacilli isolated from nasopharynx of children attending day-care centers in goiânia (brazil). p. aeruginosa (100.0%), e. coli (50.0%), k. pneumoniae (35.3%), and e. aerogenes (16.7%) were the most frequent multi-drug resistant microorganisms isolated. no production of esbl was detected.	2010	24031458
information processing without brains--the power of intercellular regulators in plants.	plants exhibit different developmental strategies than animals; these are characterized by a tight linkage between environmental conditions and development. as plants have neither specialized sensory organs nor a nervous system, intercellular regulators are essential for their development. recently, major advances have been made in understanding how intercellular regulation is achieved in plants on a molecular level. plants use a variety of molecules for intercellular regulation: hormones are us ...	2010	20332147
potential applications of immobilized β-galactosidase in food processing industries.	the enzyme β-galactosidase can be obtained from a wide variety of sources such as microorganisms, plants, and animals. the use of β-galactosidase for the hydrolysis of lactose in milk and whey is one of the promising enzymatic applications in food and dairy processing industries. the enzyme can be used in either soluble or immobilized forms but the soluble enzyme can be used only for batch processes and the immobilized form has the advantage of being used in batch wise as well as in continuous o ...	2010	21234407
antimicrobial resistance of aerobes and facultative anaerobes isolated from the oral cavity.	this study evaluated the resistance to antimicrobials of aerobes and facultative anaerobes isolated from patients wearing complete dentures, patients with gingivitis and periodontitis, and periodontally health subjects.	2010	21308284
metabolism and function of phenazines in bacteria: impacts on the behavior of bacteria in the environment and biotechnological processes.	phenazines constitute a large group of nitrogen-containing heterocyclic compounds produced by a diverse range of bacteria. both natural and synthetic phenazine derivatives are studied due their impacts on bacterial interactions and biotechnological processes. phenazines serve as electron shuttles to alternate terminal acceptors, modify cellular redox states, act as cell signals that regulate patterns of gene expression, contribute to biofilm formation and architecture, and enhance bacterial surv ...	2010	20352425
the nonribosomal peptide synthetase enzyme ddad tethers n(β)-fumaramoyl-l-2,3-diaminopropionate for fe(ii)/α-ketoglutarate-dependent epoxidation by ddac during dapdiamide antibiotic biosynthesis.	the gene cluster from pantoea agglomerans responsible for biosynthesis of the dapdiamide antibiotics encodes an adenylation-thiolation didomain protein, ddad, and an fe(ii)/α-ketoglutarate-dependent dioxygenase homologue, ddac. here we show that ddad, a nonribosomal peptide synthetase module, activates and sequesters n(β)-fumaramoyl-l-2,3-diaminopropionate as a covalently tethered thioester for subsequent oxidative modification of the fumaramoyl group. ddac catalyzes fe(ii)- and α-ketoglutarate- ...	2010	20945916
a persistent and diverse airway microbiota present during chronic obstructive pulmonary disease exacerbations.	acute exacerbations of chronic obstructive pulmonary disease (copd) are a major source of morbidity and contribute significantly to healthcare costs. although bacterial infections are implicated in nearly 50% of exacerbations, only a handful of pathogens have been consistently identified in copd airways, primarily by culture-based methods, and the bacterial microbiota in acute exacerbations remains largely uncharacterized. the aim of this study was to comprehensively profile airway bacterial com ...	2010	20141328
development of an efficient real-time quantitative pcr protocol for detection of xanthomonas arboricola pv. pruni in prunus species.	xanthomonas arboricola pv. pruni, the causal agent of bacterial spot disease of stone fruit, is considered a quarantine organism by the european union and the european and mediterranean plant protection organization (eppo). the bacterium can undergo an epiphytic phase and/or be latent and can be transmitted by plant material, but currently, only visual inspections are used to certify plants as being x. arboricola pv. pruni free. a novel and highly sensitive real-time taqman pcr detection protoco ...	2010	21037298
development of an efficient real-time quantitative pcr protocol for detection of xanthomonas arboricola pv. pruni in prunus species.	xanthomonas arboricola pv. pruni, the causal agent of bacterial spot disease of stone fruit, is considered a quarantine organism by the european union and the european and mediterranean plant protection organization (eppo). the bacterium can undergo an epiphytic phase and/or be latent and can be transmitted by plant material, but currently, only visual inspections are used to certify plants as being x. arboricola pv. pruni free. a novel and highly sensitive real-time taqman pcr detection protoco ...	2010	21037298
the bacterial community in 'taberna' a traditional beverage of southern mexico.	to characterize the bacterial community of taberna, an alcoholic traditional beverage from the southern part of mexico produced by the fermentation of the coyol palm sap (acrocomia aculeate).	2010	21039665
micro ecosystems from feed industry surfaces: a survival and biofilm study of salmonella versus host resident flora strains.	the presence of salmonella enterica serovars in feed ingredients, products and processing facilities is a well recognized problem worldwide. in norwegian feed factories, strict control measures are implemented to avoid establishment and spreading of salmonella throughout the processing chain. there is limited knowledge on the presence and survival of the resident microflora in feed production plants. information on interactions between salmonella and other bacteria in feed production plants and ...	2010	21044298
a case of pantoea endophthalmitis.	a previously healthy 50-year-old man was transferred to our hospital for evaluation of acute inflammation in his right eye after ocular trauma while using a grass mower. slit lamp examination showed 1 mm-length full thickness corneal laceration without leakage, 4+ cells and inflammatory membrane in the anterior chamber, 10% hypopyon, posterior synechiae formation, and cataract change. upon orbital computerized tomography, a metallic intraocular foreign body in the lens was identified. vitrectomy ...	2010	21052515
rmtd2, a new allele of a 16s rrna methylase gene, has been present in enterobacteriaceae isolates from argentina for more than a decade.	the first allele of a 16s rrna methyltransferase gene, rmtd2, conferring very high resistance to all clinically available aminoglycosides, was detected in 7/1,064 enterobacteria collected in 2007. rmtd2 was located on a conjugative plasmid in a tn2670-like element inside a structure similar to that of rmtd1 but probably having an independent assembly. rmtd2 has been found since 1996 to 1998 mainly in enterobacter and citrobacter isolates, suggesting a possible reservoir in these genera. this pre ...	2010	21078935
rmtd2, a new allele of a 16s rrna methylase gene, has been present in enterobacteriaceae isolates from argentina for more than a decade.	the first allele of a 16s rrna methyltransferase gene, rmtd2, conferring very high resistance to all clinically available aminoglycosides, was detected in 7/1,064 enterobacteria collected in 2007. rmtd2 was located on a conjugative plasmid in a tn2670-like element inside a structure similar to that of rmtd1 but probably having an independent assembly. rmtd2 has been found since 1996 to 1998 mainly in enterobacter and citrobacter isolates, suggesting a possible reservoir in these genera. this pre ...	2010	21078935
paenibacillus polymyxa jb05-01-1 and its perspectives for food conservation and medical applications.	the aim of this study was to isolate a novel bacterial strain with strong and broad spectrum antibacterial activity from a livestock feed prebiotic supplement. a novel strain, termed paenibacillus polymyxa jb05-01-1, was isolated using traditional microbiological methods and identified on the basis of its phenotypic and biochemical properties as well as its 16s rrna gene sequence. this strain was able to inhibit growth of gram-negative bacteria including escherichia coli rr1, pseudomonas fluores ...	2010	21132276
water microbiology. bacterial pathogens and water.	water is essential to life, but many people do not have access to clean and safe drinking water and many die of waterborne bacterial infections. in this review a general characterization of the most important bacterial diseases transmitted through water-cholera, typhoid fever and bacillary dysentery-is presented, focusing on the biology and ecology of the causal agents and on the diseases' characteristics and their life cycles in the environment. the importance of pathogenic escherichia coli str ...	2010	21139855
use of a foam spatula for sampling surfaces after bioaerosol deposition.	the present study had three goals: (i) to evaluate the relative quantities of aerosolized bacillus atrophaeus spores deposited on the vertical, horizontal top, and horizontal bottom surfaces in a chamber; (ii) to assess the relative recoveries of the aerosolized spores from glass and stainless steel surfaces with a polyester swab and a macrofoam sponge wipe; and (iii) to estimate the relative recovery efficiencies of aerosolized b. atrophaeus spores and pantoea agglomerans using a foam spatula a ...	2010	20023101
production of 3,4-dihydroxy l-phenylalanine by a newly isolated aspergillus niger and parameter significance analysis by plackett-burman design.	the amino acid derivative 3,4-dihydroxy l-phenylalanine (l-dopa) is gaining interest as a drug of choice for parkinson's disease. aspergillus oryzae is commonly used for l-dopa production; however, a slower growth rate and relatively lower tyrosinase activity of mycelia have led to an increasing interest in exploiting alternative fungal cultures. in the present investigation, we report on the microbiological transformation of l-tyrosine to l-dopa accomplished by a newly isolated filamentous fung ...	2010	21143944
bioconversion of d-galacturonate to keto-deoxy-l-galactonate (3-deoxy-l-threo-hex-2-ulosonate) using filamentous fungi.	the d-galacturonic acid derived from plant pectin can be converted into a variety of other chemicals which have potential use as chelators, clarifiers, preservatives and plastic precursors. among these is the deoxy-keto acid derived from l-galactonic acid, keto-deoxy-l-galactonic acid or 3-deoxy-l-threo-hex-2-ulosonic acid. the keto-deoxy sugars have been found to be useful precursors for producing further derivatives. keto-deoxy-l-galactonate is a natural intermediate in the fungal d-galacturon ...	2010	20796274
reconstructed ancestral sequences improve pathogen identification using resequencing dna microarrays.	we describe the benefit of using reconstructed ancestral sequences (ras) on resequencing microarrays for rapid pathogen identification, with enterobacteriaceae rpob sequences as a model. our results demonstrate a sharp improvement of call rate and accuracy when using rass as compared to extant sequences. this improvement was attributed to the lower sequence divergence of rass, which also expanded the sequence space covered by the microarray. extension of this novel microarray design strategy to ...	2010	21187950
methicillin resistance of staphylococcus species among health care and nonhealth care workers undergoing cataract surgery.	the purpose of this study is to characterize the bacterial flora of the ocular and periocular surface in cataract surgery patients and to determine the prevalence of methicillin resistance among staphylococcal isolates obtained from health care workers (hcws) and non-hcws.	2010	21191448
phyloproteomic classification of unsequenced organisms by top-down identification of bacterial proteins using caplc-ms/ms on an orbitrap.	currently, most ms-based proteomic studies of bacteria and archea match experimental data to known amino acid sequences from the target organism. top-down studies use a protein's molecular weight along with data gathered from ms/ms experiments to identify proteins by database matching. for erwinia herbicola and enterobacter cloacae, studied here, the necessary protein sequences are not available in protein sequence repositories. we apply top-down protein fragmentation, but match the experimental ...	2010	20845332
recovery from immunosuppression-related disorders in humans and animals by ip-pa1, an edible lipopolysaccharide.	immunopotentiator from pantoea agglomerans 1 (ip-pa1), an edible lipopolysaccharide (lps) derived from symbiotic bacteria in crops, is a promising immunomodulator. it activates macrophages and protects from chemotherapeutic agent-induced growth inhibition in macrophages in vitro. we showed the immune-recovery effects of ip-pa1 in a chicken model of dexamethasone-induced stress in which ip-pa1 inhibited thymic and bursal atrophy and improved antibody production in response to vaccination. further ...	2010	20871028
preparation of lipopolysaccharide derived from pantoea agglomerans labeled with fluorescence as a tracer for kinetics analysis.	intradermal and/or oral administration of lipopolysaccharides derived from pantoea agglomerans (ip-pa1) have shown multiple positive effects such as phylactic, anti-allergic and anti-tumor effects. it has been reported that the effects of ip-pa1 were derived from the induction of activated macrophages. however, it has not been actually clarified whether or not the orally administered ip-pa1 absorbed in the intestine reached and activated tissue macrophages. the aim of this study was to prepare a ...	2010	20871034
alimentary tract bacteria isolated and identified with api-20e and molecular cloning techniques from australian tropical fruit flies, bactrocera cacuminata and b. tryoni.	bacteria were isolated from the crop and midgut of field collected bactrocera cacuminata (hering) and bactrocera tryoni (froggatt) (diptera: tephritidae). two methods were used, firstly isolation onto two types of bacteriological culture media (pyea and tsa) and identification using the api-20e diagnostic kit, and secondly, analysis of samples using the 16s rrna gene molecular diagnostic method. using the api-20e method, 10 genera and 17 species of bacteria in the family enterobacteriaceae were ...	2010	20883132
inferring the evolutionary history of the plant pathogen pseudomonas syringae from its biogeography in headwaters of rivers in north america, europe, and new zealand.	nonhost environmental reservoirs of pathogens play key roles in their evolutionary ecology and in particular in the evolution of pathogenicity. in light of recent reports of the plant pathogen pseudomonas syringae in pristine waters outside agricultural regions and its dissemination via the water cycle, we have examined the genetic and phenotypic diversity, population structure, and biogeography of p. syringae from headwaters of rivers on three continents and their phylogenetic relationship to s ...	2010	20802828
[absence of mutagenic effect of pseudomonas syringae pv. atrofaciens 9400 and pantoea agglomerans p324 culture liquids].	the mutagenic activity of the culture liquids of phytopathogenic strain pseudomonas syringae pv. atrofaciens 9400 and epiphytic strain pantoea agglomerans p324 was studied in the ames test and allium cepa-test. in pro- and eucariotic test-systems no effect of the culture liquids of these bacteria on spontaneous mutations of salmonella typhimurium ta98 and ta100 or chromosome aberrations in the cells of allium cepa root apical meristem was found.	2010	20812509
molecular ecology and polyphasic characterization of the microbiota associated with semi-dry processed coffee (coffea arabica l.).	this work was aimed at isolating and identifying the microbiota present during the semi-dry method of coffee processing using polyphasic methods and to evaluate microbial diversity with pcr-dgge. samples of coffea arabica l. were collected during different processing stages in southern minas gerais, brazil. the bacterial and fungal isolates were phenotypically characterised and grouped according to the ardra technique, in which the 16-23s and its1-5.8s regions of the rdna were sequenced for spec ...	2010	20832694
gene expression profile of the plant pathogen fusarium graminearum under the antagonistic effect of pantoea agglomerans.	the pathogenic fungus fusarium graminearum is an ongoing threat to agriculture, causing losses in grain yield and quality in diverse crops. substantial progress has been made in the identification of genes involved in the suppression of phytopathogens by antagonistic microorganisms; however, limited information regarding responses of plant pathogens to these biocontrol agents is available. gene expression analysis was used to identify differentially expressed transcripts of the fungal plant path ...	2010	20623455
1,3-propanediol production potential of clostridium saccharobutylicum nrrl b-643.	owing to the significant interest in biofuel production in the form of biodiesel, vast amount of glycerol as a waste product is produced all over the world. among the economically viable and ecologically acceptable solutions for the safe disposal of this waste, biotechnological conversion of glycerol into a valuable bioplastic raw material, namely 1,3-propanediol (1,3-pdo) seems to be very promising. in this study, 1,3-pdo production potential of clostridium saccharobutylicum nrrl b-643 was stud ...	2010	20647065
protective effects of the immunopotentiator from pantoea agglomerans 1 on chemotherapeutic agent-induced macrophage growth inhibition.	the immunopotentiator from pantoea agglomerans 1 (ip-pa1) is an edible lipopolysaccharide (lps) derived from symbiotic bacteria found in crops. ip-pa1 is known to ameliorate chemotherapy-induced immunosuppression; therefore, its macrophage-activating effect in the presence of chemotherapeutic agents was evaluated.	2010	20651348
identification of the corn pathogen pantoea stewartii by mass spectrometry of whole-cell extracts and its detection with novel pcr primers.	pantoea stewartii subsp. stewartii is the causative agent of stewart's wilt, a bacterial disease transmitted by the corn flea beetle mainly to sweet corn (zea mays). in many countries, it is classified as a quarantine organism and must be differentiated from other yellow enteric bacteria frequently occurring with corn. we have created novel primers from the psts-glms region of p. stewartii for use in conventional pcr (cpcr) and quantitative pcr (qpcr). to facilitate rapid diagnosis, we applied m ...	2010	20656863
sporadic pantoea agglomerans bacteremia in a near-term female: case report and review of literature.	pantoea agglomerans is a plant pathogen and an unusual cause of human disease typically associated with thorn prick injuries or contaminated parenteral fluids. in the neonate, p. agglomerans has been reported to cause bacteremia or sepsis 17 times previously. in most of these cases, the source of infection has been contaminated parenteral nutrition or has been associated with indwelling catheters. we present a rare case of p. agglomerans bacteremia in a 35-week-female born via vaginal delivery c ...	2010	20657073
culturable bacterial microflora associated with nectarine fruit and their potential for control of brown rot.	microflora of fruit surfaces have been the best source of antagonists against fungi causing postharvest decay of fruit. however, there is little information on microflora colonizing surfaces of fruits other than grape, apple, and citrus. we characterized bacterial microflora on nectarine fruit surfaces from the early stage of development until harvest. identification of bacterial strains was made using midi (fatty acid methyl ester analysis) and biolog systems. biolog identified 35% and midi 53% ...	2010	20657618
anaerobic biotransformation of high concentrations of chloroform by an enrichment culture and two bacterial isolates.	a fermentative enrichment culture (designated dhm-1) was developed that is capable of cometabolically biotransforming high concentrations of chloroform (cf) to nontoxic end products. two pantoea spp. were isolated from dhm-1 that also possess this dechlorination capability. following acclimation to increasing levels of cf, corn syrup-grown dhm-1 was able to transform over 500 mg/liter cf in the presence of vitamin b(12) (approximately 3% of cf on a molar basis) at a rate as high as 22 mg/liter/d ...	2010	20693443
production of folate by bacteria isolated from oat bran.	twenty bacteria isolated from three commercial oat bran products were tested for their folate production capability. the bacteria as well as some reference organisms were grown until early stationary phase on a rich medium (ypd), and the amount of total folate in the separated cell mass and the culture medium (supernatant) was determined by microbiological assay. folate vitamer distribution was determined for eight bacteria including one isolated from rye flakes. for seven bacteria the effect of ...	2010	20708290
a case of bilateral endogenous pantoea agglomerans endophthalmitis with interstitial lung disease.	we here in report a case of bilateral endogenous endophthalmitis caused by pantoea agglomerans (p. agglomerans) in a patient who had interstitial lung disease and was treated with oral corticosteroids. a 72-year-old man presented with decreased visual acuity in both eyes nine days after he received oral corticosteroids. he had marked uveitis, cataracts, and vitreous opacities. cultures were taken of blood, aqueous humor, and vitreous. we initially suspected a fungal etiology and treated him with ...	2010	20714391
detection of airborne bacteria in a german turkey house by cultivation-based and molecular methods.	today's large-scale poultry production with densely stocked and enclosed production buildings is often accompanied by very high concentrations of airborne microorganisms leading to a clear health hazard for employees working in such environments. depending on the expected exposure to microorganisms, work has to be performed under occupational safety conditions. in this study, turkey houses bioaerosols were investigated by cultivation-based and molecular methods in parallel to determine the conce ...	2010	20720091
genome sequence of the biocontrol agent pantoea vagans strain c9-1.	pantoea vagans is a gram-negative enterobacterial plant epiphyte of a broad range of plants. here we report the 4.89-mb genome sequence of p. vagans strain c9-1 (formerly pantoea agglomerans), which is commercially registered for biological control of fire blight, a disease of pear and apple trees caused by erwinia amylovora.	2010	20952567
etiology and antibacterial susceptibility pattern of community-acquired bacterial ocular infections in a tertiary eye care hospital in south india.	to identify the etiology, incidence and prevalence of ocular bacterial infections, and to assess the in vitro susceptibility of these ocular bacterial isolates to commonly used antibiotics.	2010	20952834
biodegradation of polyether algal toxins--isolation of potential marine bacteria.	marine algal toxins such as brevetoxins, okadaic acid, yessotoxin, and ciguatoxin are polyether compounds. the fate of polyether toxins in the aqueous phase, particularly bacterial biotransformation of the toxins, is poorly understood. an inexpensive and easily available polyether structural analog salinomycin was used for enrichment and isolation of potential polyether toxin degrading aquatic marine bacteria from florida bay area, and from red tide endemic sites in the south florida gulf coast. ...	2010	20954040
[isolation, identification and characterization of acc deaminase-containing endophytic bacteria from halophyte suaeda salsa].	we isolated and characterized 1-aminocyclopropane-1-carboxylate (acc) deaminase-containing endophytic bacteria from halophyte suaeda salsa to understand the interactions between endophytes and halophyte.	2010	21268896
immune recovery effects of immunopotentiator from pantoea agglomerans 1 (ip-pa1) on low antibody productions in response to salmonella enteritidis vaccine and sheep red blood cells in dexamethasone-treated stressed chicken models.	considering the usefulness of the immunopotentiator from pantoea agglomerans 1 (ip-pa1), which is a purified lipopolysaccharide (lps) derived from symbiotic gram-negative bacteria of food crops, in controlling immunosuppression in poultry husbandry, in this study, we examined its immune-recovery effects in dexamethasone-treated stressed chicken models. three-week-old chickens daily administered 10 microg/kg of dexamethasone for 35 days to induce stress showed more whole body weight loss; relativ ...	2010	20035113
technology-induced selection towards the spoilage microbiota of artisan-type cooked ham packed under modified atmosphere.	the microbiota associated with a highly-perishable belgian artisan-type cooked ham was analyzed through plating and (gtg)(5)-fingerprinting of isolates throughout its processing chain. the raw tumbled meat was characterized by the presence of a versatile microbiota around 4.8 log(cfu g(-1)), consisting of lactic acid bacteria, staphylococci, brochothrix thermosphacta, gram-negative bacteria, and yeasts. pasteurisation of the ham logs reduced bacterial counts below 2 log(cfu g(-1)) and subsequent ...	2010	19913696
bacteria colonizing root nodules of wild legumes exhibit virulence-associated properties of mammalian pathogens.	bacteria not proficient in nitrogen fixing symbiosis were proven able to invade root nodules of three wild legumes of the genus hedysarum in algeria and to be multiplying in these in place of the natural rhizobium symbionts. the involved species featured taxa known as human pathogens including: enterobacter cloacae, enterobacter kobei, escherichia vulneris, pantoea agglomerans and leclercia adecarboxylata. a direct screening of the phenotypic determinants of virulence using human cultured cells ...	2010	19916054
emended description of the genus pantoea, description of four species from human clinical samples, pantoea septica sp. nov., pantoea eucrina sp. nov., pantoea brenneri sp. nov. and pantoea conspicua sp. nov., and transfer of pectobacterium cypripedii (hori 1911) brenner et al. 1973 emend. hauben et al. 1998 to the genus as pantoea cypripedii comb. nov.	bacterial strains belonging to dna hybridization groups (hg) ii, iv and v, in the erwinia herbicola-enterobacter agglomerans complex, of brenner et al. [int j syst bacteriol 34 (1984), 45-55] were suggested previously to belong to the genus pantoea, but have never been formally described and classified. additionally, it has been shown in several studies that pectobacterium cypripedii is more closely related to species of pantoea than to those of pectobacterium. in this study, the phylogenetic po ...	2010	19946052
discrete site surface complexation constants for lanthanide adsorption to bacteria as determined by experiments and linear free energy relationships.	bacteria are abundant in many natural and engineered environments where they are thought to exert important controls on the cycling, mobility, bioavailability, and toxicity of metal contaminants. in order to probe their role in moderating the behavior of lanthanides, ph-dependent adsorption edges of 13 individual lanthanides and yttrium to the gram-negative bacterium pantoea agglomerans were used to generate discrete site surface complexation constants. the calculated surface complexation consta ...	2010	20000843
discovery of 3-formyl-tyrosine metabolites from pseudoalteromonas tunicata through heterologous expression.	genome mining and identification of natural product gene clusters typically relies on the presence of canonical nonribosomal polypeptide synthetase (nrps) or polyketide synthase (pks) domains. recently, other condensation enzymes, such as the atp-grasp ligases, have been recognized as important players in natural product biosynthesis. in this study, sequence based searching for homologues of ddaf, the atp-grasp amide ligase from dapdiamide biosynthesis, led to the identification of a previously ...	2010	20041686
discovery of 3-formyl-tyrosine metabolites from pseudoalteromonas tunicata through heterologous expression.	genome mining and identification of natural product gene clusters typically relies on the presence of canonical nonribosomal polypeptide synthetase (nrps) or polyketide synthase (pks) domains. recently, other condensation enzymes, such as the atp-grasp ligases, have been recognized as important players in natural product biosynthesis. in this study, sequence based searching for homologues of ddaf, the atp-grasp amide ligase from dapdiamide biosynthesis, led to the identification of a previously ...	2010	20041686
carob pulp as raw material for production of the biocontrol agent p. agglomerans pbc-1.	large-scale production has been the major obstacle to the success of many biopesticides. the spreading of microbial biocontrol agents against postharvest disease, as a safe and environmentally friendly alternative to synthetic fungicides, is quite dependent on their industrial mass production from low-cost raw materials. considerable interest has been shown in using agricultural waste products and by-products from food industry as nitrogen and carbon sources. in this work, carob pulp aqueous ext ...	2010	20593299
[isolation and characterization of a facultative anaerobe pantoea agglomerans mfc-3 and its humic substance-and fe(iii) - respiring activity].	a strain of humic substance- and fe(iii)- reducing bacterium was isolated from the subterranean forest sediment and designated as mfc-3. the strain is facultative anaerobic, gram-negative, motile and rod (1.0-3.0 microm long, 0.5-1.0 microm wide) and identified as pantoea agglomerans with the 16s rdna sequence analyses. batch experiments were conducted to investigate its humic substance-and fe(iii)-respiring activity. the results showed that mfc-3 was capable of anaerobic respiration on anthraqu ...	2010	20329545
diversity of fecal coliforms and their antimicrobial resistance patterns in wastewater treatment model plant.	the occurrence of resistance patterns among wastewater fecal coliforms was determined in the study. susceptibility of the isolates was tested against 19 antimicrobial agents: aminoglycosides, aztreonam, carbapenems, cephalosporines, beta-lactam/beta-lactamase inhibitors, penicillines, tetracycline, trimethoprim/sulfamethoxazole, and fluoroquinolones. additionally the removal of resistant isolates was evaluated in the laboratory-scale wastewater treatment model plant (m-wwtp), continuously suppli ...	2010	20351416
application of whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid identification and clustering analysis of pantoea species.	pantoea agglomerans is an ecologically diverse taxon that includes commercially important plant-beneficial strains and opportunistic clinical isolates. standard biochemical identification methods in diagnostic laboratories were repeatedly shown to run into false-positive identifications of p. agglomerans, a fact which is also reflected by the high number of 16s rrna gene sequences in public databases that are incorrectly assigned to this species. more reliable methods for rapid identification ar ...	2010	20453125
dapdiamides, tripeptide antibiotics formed by unconventional amide ligases.	construction of a genomic dna library from pantoea agglomerans strain cu0119 and screening against the plant pathogen erwinia amylovora yielded a new family of antibiotics, dapdiamides a-e (1-5). the structures were established through 2d-nmr experiments and mass spectrometry, as well as the synthesis of dapdiamide a (1). transposon mutagenesis of the active cosmid allowed identification of the biosynthetic gene cluster. the dapdiamide family's promiscuous biosynthetic pathway contains two uncon ...	2010	20041689
new blue pigment produced by pantoea agglomerans and its production characteristics at various temperatures.	a bacterium capable of producing a deep blue pigment was isolated from the environment and identified as pantoea agglomerans. the pigment production characteristics of the bacterium under various conditions were studied. the optimal agar plate ingredients for pigment production by the bacterium were first studied: the optimal ingredients were 5 g/liter glucose, 10 g/liter tryptic soy broth, and 40 g/liter glycerol at ph 6.4. bacterial cells grew on the agar plate during the incubation, while the ...	2010	20971865
long-term epidemiology of bacterial susceptibility profiles in adults suffering from febrile neutropenia with hematologic malignancy after antibiotic change.	the aim of this study was to investigate the epidemiology and antibiotic susceptibility profiles of isolated bacterial organisms in relation to empiric treatment of neutropenic fever over a 15-year period.	2010	21694894
influencing factors in the occurrence of injured coliforms in the drinking water distribution system in the city of la plata, argentina.	the objective of this study was to evaluate the influencing factors in the occurrence of coliforms in the drinking water in la plata (argentina) from june 1999 to june 2001. a total of 180 samples were collected from rio de la plata (102 samples) and puelche aquifer (78 samples); 45 samples were collected for each of the four seasons. the membrane filter procedure was used for isolating bacteria, and each sample was tested for chlorine and ph. the highest percentage of samples positive for colif ...	2010	20154384
characterization of a broad-host-range flagellum-dependent phage that mediates high-efficiency generalized transduction in, and between, serratia and pantoea.	a phage (phiot8) isolated on serratia sp. atcc 39006 was shown to be flagellum-dependent, and to mediate generalized transduction with high efficiency (up to 10(-4) transductants per p.f.u.). phiot8 was shown to have a broad host range because it also infected a strain of pantoea agglomerans isolated from the rhizosphere. transduction of plasmid-borne antibiotic resistance between the two bacterial genera was demonstrated, consistent with purported ecological roles of phages in dissemination of ...	2010	19778959
structural analysis of the lipid a isolated from hafnia alvei 32 and pcm 1192 lipopolysaccharides.	hafnia alvei, a gram-negative bacterium, is an opportunistic pathogen associated with mixed hospital infections, bacteremia, septicemia, and respiratory diseases. the majority of clinical symptoms of diseases caused by this bacterium have a lipopolysaccharide (lps, endotoxin)-related origin. the lipid a structure affects the biological activity of endotoxins predominantly. thus, the structure of h. alvei lipid a was analyzed for the first time. the major form, asymmetrically hexa-acylated lipid ...	2010	19706748
transgenesis and paratransgenesis to control insect-borne diseases: current status and future challenges.	insect-borne diseases cause significant human morbidity and mortality. current control and preventive methods against vector-borne diseases rely mainly on insecticides. the emergence of insecticide resistance in many disease vectors highlights the necessity to develop new strategies to control these insects. vector transgenesis and paratransgenesis are novel strategies that aim at reducing insect vectorial capacity, or seek to eliminate transmission of pathogens such as plasmodium sp., trypanoso ...	2010	19819346
transgenesis and paratransgenesis to control insect-borne diseases: current status and future challenges.	insect-borne diseases cause significant human morbidity and mortality. current control and preventive methods against vector-borne diseases rely mainly on insecticides. the emergence of insecticide resistance in many disease vectors highlights the necessity to develop new strategies to control these insects. vector transgenesis and paratransgenesis are novel strategies that aim at reducing insect vectorial capacity, or seek to eliminate transmission of pathogens such as plasmodium sp., trypanoso ...	2010	19819346
isolation and functional characterization of a lycopene beta-cyclase gene that controls fruit colour of papaya (carica papaya l.).	the colour of papaya fruit flesh is determined largely by the presence of carotenoid pigments. red-fleshed papaya fruit contain lycopene, whilst this pigment is absent from yellow-fleshed fruit. the conversion of lycopene (red) to beta-carotene (yellow) is catalysed by lycopene beta-cyclase. this present study describes the cloning and functional characterization of two different genes encoding lycopene beta-cyclases (lcy-beta1 and lcy-beta2) from red (tainung) and yellow (hybrid 1b) papaya cult ...	2010	19887502
isolation and functional characterization of a lycopene beta-cyclase gene that controls fruit colour of papaya (carica papaya l.).	the colour of papaya fruit flesh is determined largely by the presence of carotenoid pigments. red-fleshed papaya fruit contain lycopene, whilst this pigment is absent from yellow-fleshed fruit. the conversion of lycopene (red) to beta-carotene (yellow) is catalysed by lycopene beta-cyclase. this present study describes the cloning and functional characterization of two different genes encoding lycopene beta-cyclases (lcy-beta1 and lcy-beta2) from red (tainung) and yellow (hybrid 1b) papaya cult ...	2010	19887502
cytochrome d but not cytochrome o rescues the toluidine blue growth sensitivity of arc mutants of escherichia coli.	the arc (anoxic redox control) two-component signal transduction system, consisting of the arcb sensor kinase and the arca response regulator, allows adaptive responses of escherichia coli to changes of o(2) availability. the arca gene was previously known as the dye gene because null mutants were growth sensitive to the photosensitizer redox dyes toluidine blue and methylene blue, a phenotype whose molecular basis still remains elusive. in this study we report that the toluidine blue o (tbo) ef ...	2010	19897650
cytochrome d but not cytochrome o rescues the toluidine blue growth sensitivity of arc mutants of escherichia coli.	the arc (anoxic redox control) two-component signal transduction system, consisting of the arcb sensor kinase and the arca response regulator, allows adaptive responses of escherichia coli to changes of o(2) availability. the arca gene was previously known as the dye gene because null mutants were growth sensitive to the photosensitizer redox dyes toluidine blue and methylene blue, a phenotype whose molecular basis still remains elusive. in this study we report that the toluidine blue o (tbo) ef ...	2010	19897650
micro-scale water potential gradients visualized in soil around plant root tips using microbiosensors.	water availability and movement in soil are critical determinants of resource availability to, and interactions among, members of the soil community. however, it has been impossible to observe gradients in soil water potential empirically at millimetre spatial scales. here we describe progress towards that goal using output from two microbial biosensors, pantoea agglomerans brt98/ppprogreen and pseudomonas putida kt2442/ppprogreen, engineered with a reporter system based on the osmotically sensi ...	2010	19906152
retained hawthorn fragment in a child's foot complicated by infection: diagnosis and excision aided by localization with ultrasound.	puncture wounds in children are very common and often result in foreign body retention. organic materials in the form of plant thorns present problems in identification and localization because they are not visualized with plain radiographs. a case of a 10-year-old girl with a small piece of retained hawthorn is presented. correct diagnosis and treatment were delayed because of misinterpretation of magnetic resonance image studies. ultrasound ultimately located the foreign body, and assisted in ...	2010	19962326
the purb gene controls rhizosphere colonization by pantoea agglomerans.	to isolate the rhizosphere competence-defective transposon tn5 mutant of pantoea agglomerans nbrisrm (srm) and to identify the gene causing defect in its root colonization ability.	2010	20002573
diversity and evolution of the phenazine biosynthesis pathway.	phenazines are versatile secondary metabolites of bacterial origin that function in biological control of plant pathogens and contribute to the ecological fitness and pathogenicity of the producing strains. in this study, we employed a collection of 94 strains having various geographic, environmental, and clinical origins to study the distribution and evolution of phenazine genes in members of the genera pseudomonas, burkholderia, pectobacterium, brevibacterium, and streptomyces. our results con ...	2010	20008172
diversity and evolution of the phenazine biosynthesis pathway.	phenazines are versatile secondary metabolites of bacterial origin that function in biological control of plant pathogens and contribute to the ecological fitness and pathogenicity of the producing strains. in this study, we employed a collection of 94 strains having various geographic, environmental, and clinical origins to study the distribution and evolution of phenazine genes in members of the genera pseudomonas, burkholderia, pectobacterium, brevibacterium, and streptomyces. our results con ...	2010	20008172
slow onset inhibition of bacterial beta-ketoacyl-acyl carrier protein synthases by thiolactomycin.	thiolactomycin (tlm), a natural product thiolactone antibiotic produced by species of nocardia and streptomyces, is an inhibitor of the beta-ketoacyl-acyl carrier protein synthase (kas) enzymes in the bacterial fatty acid synthase pathway. using enzyme kinetics and direct binding studies, tlm has been shown to bind preferentially to the acyl-enzyme intermediates of the kasi and kasii enzymes from mycobacterium tuberculosis and escherichia coli. these studies, which utilized acyl-enzyme mimics in ...	2010	20018879
slow onset inhibition of bacterial beta-ketoacyl-acyl carrier protein synthases by thiolactomycin.	thiolactomycin (tlm), a natural product thiolactone antibiotic produced by species of nocardia and streptomyces, is an inhibitor of the beta-ketoacyl-acyl carrier protein synthase (kas) enzymes in the bacterial fatty acid synthase pathway. using enzyme kinetics and direct binding studies, tlm has been shown to bind preferentially to the acyl-enzyme intermediates of the kasi and kasii enzymes from mycobacterium tuberculosis and escherichia coli. these studies, which utilized acyl-enzyme mimics in ...	2010	20018879
genes involved in yellow pigmentation of cronobacter sakazakii es5 and influence of pigmentation on persistence and growth under environmental stress.	cronobacter spp. are opportunistic food-borne pathogens that are responsible for rare but highly fatal cases of meningitis and necrotizing enterocolitis in neonates. while the operon responsible for yellow pigmentation in cronobacter sakazakii strain es5 was described recently, the involvement of additional genes in pigment expression and the influence of pigmentation on the fitness of cronobacter spp. have not been investigated. thus, the aim of this study was to identify further genes involved ...	2010	20038705
genes involved in yellow pigmentation of cronobacter sakazakii es5 and influence of pigmentation on persistence and growth under environmental stress.	cronobacter spp. are opportunistic food-borne pathogens that are responsible for rare but highly fatal cases of meningitis and necrotizing enterocolitis in neonates. while the operon responsible for yellow pigmentation in cronobacter sakazakii strain es5 was described recently, the involvement of additional genes in pigment expression and the influence of pigmentation on the fitness of cronobacter spp. have not been investigated. thus, the aim of this study was to identify further genes involved ...	2010	20038705