Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| regt can modulate gingipain activity and response to oxidative stress in porphyromonas gingivalis. | recombinant vima protein can interact with the gingipains and several other proteins that may play a role in its biogenesis in porphyromonas gingivalis. in silico analysis of pg2096, a hypothetical protein that was shown to interact with vima, suggests that it may have environmental stress resistance properties. to further evaluate the role(s) of pg2096, the predicted open reading frame was pcr amplified from p. gingivalis w83 and insertionally inactivated using the ermf-ermam antibiotic-resista ... | 2010 | 20595264 |
| complement and periodontitis. | although the complement system is centrally involved in host defense, its overactivation or deregulation (e.g., due to inherent host genetic defects or due to pathogen subversion) may excessively amplify inflammation and contribute to immunopathology. periodontitis is an oral infection-driven chronic inflammatory disease which exerts a systemic impact on health. this paper reviews evidence linking complement to periodontal inflammation and pathogenesis. clinical and histological observations sho ... | 2010 | 20599785 |
| the hpk2-rrp2 two-component regulatory system of treponema denticola: a potential regulator of environmental and adaptive responses. | treponema denticola levels in the gingival crevice become elevated as periodontal disease develops. oral treponemes may account for as much as 40% of the total bacterial population in the periodontal pocket. the stimuli that trigger enhanced growth of t. denticola, and the mechanisms associated with the transmission of these signals, remain to be defined. we hypothesize that the t. denticola open reading frames tde1970 (histidine kinase) and tde1969 (response regulator) constitute a functional t ... | 2010 | 20618698 |
| molecular characterization of treponema denticola infection-induced bone and soft tissue transcriptional profiles. | treponema denticola is associated with subgingival biofilms in adult periodontitis and with acute necrotizing ulcerative gingivitis. however, the molecular mechanisms by which t. denticola impacts periodontal inflammation and alveolar bone resorption remain unclear. here, we examined changes in the host transcriptional profiles during a t. denticola infection using a murine calvarial model of inflammation and bone resorption. t. denticola was injected into the subcutaneous soft tissue over the c ... | 2010 | 20618700 |
| differential host response to lps variants in amniochorion and the tlr4/md-2 system in macaca nemestrina. | microbial-specific factors are likely critical in determining whether bacteria trigger preterm labor. structural variations in lipopolysaccharide (lps), a component of gram-negative bacteria, can determine whether lps has an inflammatory (agonist) or anti-inflammatory (antagonist) effect through toll-like receptor 4 (tlr4). our objective was to determine whether amniochorion could discriminate between lps variants in a nonhuman primate model. we also cloned macaca nemestrina tlr4 and md-2 and co ... | 2010 | 20619890 |
| dentipain, a streptococcus pyogenes ides protease homolog, is a novel virulence factor of treponema denticola. | treponema denticola is a major pathogen of chronic periodontitis. analysis of the t. denticola genome revealed a gene orthologous with a cysteine protease-encoding gene from streptococcus pyogenes (ides). ides interferes with igg-dependent opsonophagocytosis by specific cleavage of igg molecules. analysis of this gene (termed idet) revealed it to encode a two-domain protein whose n-terminus is composed of tandem immunoglobulin-like domains followed by a c-terminal ides-like protease domain. in t ... | 2010 | 20635859 |
| "gum bug, leave my heart alone!"--epidemiologic and mechanistic evidence linking periodontal infections and atherosclerosis. | evidence from epidemiologic studies suggests that periodontal infections are independently associated with subclinical and clinical atherosclerotic vascular disease. although the strength of the reported associations is modest, the consistency of the data across diverse populations and a variety of exposure and outcome variables suggests that the findings are not spurious or attributable only to the effects of confounders. analysis of limited data from interventional studies suggests that period ... | 2010 | 20639510 |
| espp, a serine protease of enterohemorrhagic escherichia coli, impairs complement activation by cleaving complement factors c3/c3b and c5. | hemolytic-uremic syndrome (hus) is a life-threatening disorder characterized by hemolytic anemia, thrombocytopenia, and renal insufficiency. it is caused mainly by infections with enterohemorrhagic escherichia coli (ehec). recently, shiga toxin 2, the best-studied virulence factor of ehec, was reported to interact with complement, implying that complement may be involved in the pathogenesis of ehec-induced hus. the aim of the present study was to investigate whether or not the serine protease es ... | 2010 | 20643852 |
| examination of periodontal pathogens in stenotic valve specimens and in whole blood samples in patients affected by aortic valve stenosis and chronic periodontitis. | periodontitis may be a risk factor for atherosclerosis and coronary heart disease. the influence of periodontal pathogens in cardiovascular diseases needs further investigation. therefore, the aims of this clinical study are: to test the presence of periodontal bacteria dna in aortic valves and to assess the concomitant presence of the same periodontal bacteria dna in whole blood samples in patients affected by aortic valve stenosis and chronic periodontitis. nineteen consecutive patients (12 ma ... | 2010 | 20646351 |
| 16s rrna-based detection of oral pathogens in coronary atherosclerotic plaque. | atherosclerosis develops as a response of the vessel wall to injury. chronic bacterial infections have been associated with an increased risk for atherosclerosis and coronary artery disease. the ability of oral pathogens to colonize in coronary atheromatous plaque is well known. | 2010 | 20657096 |
| characterization of the normal bacterial flora in peri-implant sulci of partially and completely edentulous patients. | to characterize the normal bacterial flora and evaluate the presence of periodontopathogenic bacteria around dental implants and to correlate them with the periodontal flora or, in completely edentulous patients, the alveolar gingival flora. | 2010 | 20657863 |
| the ability of the bana test to detect different levels of p. gingivalis, t. denticola and t. forsythia. | the aim of this study was to evaluate the ability of the bana test to detect different levels of porphyromonas gingivalis, treponema denticola and tannerella forsythia or their combinations in subgingival samples at the initial diagnosis and after periodontal therapy. periodontal sites with probing depths between 5-7 mm and clinical attachment level between 5-10 mm, from 53 subjects with chronic periodontitis, were sampled in four periods: initial diagnosis (t0), immediately (t1), 45 (t2) and 60 ... | 2010 | 20658043 |
| [association between phenytoin-induced gingival hyperplasia and periodontopathic bacteria in institutionalized patients with severe motor and intellectual disabilities]. | the association between phenytoin-induced gingival hyperplasia (pigh) and five major periodontopathic bacteria was investigated by real-time pcr in 12 subjects (30.6 +/- 7.95 years of age) with severe motor and intellectual disabilities. the subjects had not received antibiotics or periodontal therapy within three months. a healthy gingival site or a site with the lowest gingival hyperplasia score (minimum site), and a site with the maximum score of gingival hyperplasia (maximum site) on each su ... | 2010 | 20662307 |
| td92, an outer membrane protein of treponema denticola, induces osteoclastogenesis via prostaglandin e(2)-mediated rankl/osteoprotegerin regulation. | periodontitis is a chronic inflammatory disease of the periodontium that causes significant alveolar bone loss. osteoclasts are bone-resorbing multinucleated cells. osteoblasts regulate osteoclast differentiation by expression of rankl and osteoprotegerin (opg). td92 is a surface-exposed outer membrane protein of treponema denticola, a periodontopathogen. although it has been demonstrated that td92 acts as a stimulator of various proinflammatory mediators, the role of td92 in alveolar bone resor ... | 2010 | 20682013 |
| relationship between oral status and prevalence of periodontopathic bacteria on the tongues of elderly individuals. | colonization of periodontopathic bacteria is associated with increased risk of systemic diseases. however, few studies have investigated the relationships between oral status factors and health-related quality of life (hr-qol) and the prevalence of such bacteria in elderly individuals. this study investigated the prevalence of porphyromonas gingivalis, prevotella intermedia, treponema denticola and tannerella forsythia in 165 community-dwelling functionally independent 85-year-old japanese indiv ... | 2010 | 20688951 |
| a simplified erythromycin resistance cassette for treponema denticola mutagenesis. | the primary selectable marker for the genetic studies of treponema denticola is a hybrid gene cassette containing both ermf and ermam (ermb) genes. ermb functions in escherichia coli, while ermf has been assumed to confer resistance in t. denticola. we demonstrate here that ermb is sufficient for erythromycin selection in t. denticola and that the native ermb promoter drives ermb expression. | 2010 | 20691222 |
| dichotomy of gingipains action as virulence factors: from cleaving substrates with the precision of a surgeon's knife to a meat chopper-like brutal degradation of proteins. | 2010 | 20712631 | |
| contribution of porphyromonas gingivalis lipopolysaccharide to periodontitis. | 2010 | 20712633 | |
| virulence mechanisms of tannerella forsythia. | 2010 | 20712636 | |
| virulence factors of treponema denticola. | 2010 | 20712637 | |
| periodontal innate immune mechanisms relevant to atherosclerosis and obesity. | 2010 | 20712641 | |
| antigenic activity of bacterial endodontic contents from primary root canal infection with periapical lesions against macrophage in the release of interleukin-1beta and tumor necrosis factor alpha. | periradicular tissue chronic stimulation by endotoxin may cause apical periodontitis. this study investigated the microbial profile and the levels of endotoxin found in primary root canal infection with apical periodontitis, determined their antigenicity against macrophages through the levels of interleukin (il)-1beta and tumor necrosis factor alpha (tnf-alpha), and evaluated their relationship with clinical and radiographic findings. | 2010 | 20728711 |
| selective substitution of amino acids limits proteolytic cleavage and improves the bioactivity of an anti-biofilm peptide that targets the periodontal pathogen, porphyromonas gingivalis. | the interaction of the periodontal pathogen, porphyromonas gingivalis, with oral streptococci such as streptococcus gordonii precedes colonization of the subgingival pocket and represents a target for limiting p. gingivalis colonization of the oral cavity. previous studies showed that a synthetic peptide (designated bar) derived from the antigen i/ii protein of s. gordonii was a potent competitive inhibitor of p. gingivalis adherence to s. gordonii and subsequent biofilm formation. here we show ... | 2010 | 20800634 |
| effect of khat chewing on periodontal pathogens in subgingival biofilm from chronic periodontitis patients. | existing in vitro and in vivo data suggest that khat may have a favorable effect on periodontal microbiota. the purpose of this study was to assess the effect of khat chewing on major periodontal pathogens in subgingival plaque samples from subjects with chronic periodontitis. | 2010 | 20816745 |
| functional characterization of the incomplete phosphotransferase system (pts) of the intracellular pathogen brucella melitensis. | in many bacteria, the phosphotransferase system (pts) is a key player in the regulation of the assimilation of alternative carbon sources notably through catabolic repression. the intracellular pathogens brucella spp. possess four pts proteins (eintr, npr, eiiantr and an eiia of the mannose family) but no pts permease suggesting that this pts might serve only regulatory functions. | 2010 | 20844759 |
| cellular architecture of treponema pallidum: novel flagellum, periplasmic cone, and cell envelope as revealed by cryo electron tomography. | high-resolution cryo electron tomography (cryo-et) was utilized to visualize treponema pallidum, the causative agent of syphilis, at the molecular level. three-dimensional (3d) reconstructions from 304 infectious organisms revealed unprecedented cellular structures of this unusual member of the spirochetal family. high-resolution cryo-et reconstructions provided detailed structures of the cell envelope, which is significantly different from that of gram-negative bacteria. the 4-nm lipid bilayer ... | 2010 | 20850455 |
| clonal analysis of the microbiota of severe early childhood caries. | severe early childhood caries is a microbial infection that severely compromises the dentition of young children. the aim of this study was to characterize the microbiota of severe early childhood caries. | 2010 | 20861633 |
| surface immunolabeling and consensus computational framework to identify candidate rare outer membrane proteins of treponema pallidum. | treponema pallidum reacts poorly with the antibodies present in rabbit and human syphilitic sera, a property attributed to the paucity of proteins in its outer membrane. to better understand the basis for the syphilis spirochete's "stealth pathogenicity," we used a dual-label, 3-step amplified assay in which treponemes encapsulated in gel microdroplets were probed with syphilitic sera in parallel with anti-flaa antibodies. a small (approximately 5 to 10%) but reproducible fraction of intact trep ... | 2010 | 20876295 |
| investigation of subgingival profile of periodontopathic bacteria using polymerase chain reaction. | periodontopathic bacteria such as porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, tannerella forsythia, campylobacter rectus and treponema denticola play an important role in the initiation and progression of periodontitis. the aim of this investigation was to evaluate the relationship between periodontal clinical parameters and the subgingival profile of periodontopathic bacteria. twenty-six periodontitis patients (23-62 years of age; mean age, 40.2±13.2) with no systemic disea ... | 2010 | 20877160 |
| tannerella forsythia infection-induced calvarial bone and soft tissue transcriptional profiles. | tannerella forsythia is associated with subgingival biofilms in adult periodontitis, although the molecular mechanisms contributing to chronic inflammation and loss of periodontal bone remain unclear. we examined changes in the host transcriptional profiles during a t. forsythia infection using a murine calvarial model of inflammation and bone resorption. tannerella forsythia was injected into the subcutaneous soft tissue over calvariae of balb/c mice for 3 days, after which the soft tissues and ... | 2010 | 20883221 |
| using dgge profiling to develop a novel culture medium suitable for oral microbial communities. | more than 700 bacterial species have been detected in the human oral cavity. they form highly organized microbial communities and are responsible for many oral infectious diseases, such as dental caries and periodontal disease. the prevention and treatment of these diseases require a comprehensive knowledge of oral microbial communities, which largely relies on culture-dependent methods to provide detailed phenotypic and physiological analysis of these communities. however, most of the currently ... | 2010 | 20883224 |
| an update on periodontic-orthodontic interrelationships. | talking about periodontic-orthodontic interrelationships is related primarily to the 1960s, where a generalized increase in salivary bacterial counts, especially lactobacillus, had been shown after orthodontic band placement. the purpose of this article is to provide the dental practitioner with basic understanding of the interrelationship between periodontics and orthodontics by means of representing classical studies, and, to give an update on this topic by demonstrating the most recent opinio ... | 2010 | 20922083 |
| potential of the tannerella forsythia s-layer to delay the immune response. | the periodontal pathogen tannerella forsythia possesses a glycosylated s-layer as an outermost cell decoration. while the s-layer provides a selection advantage to the bacterium in the natural habitat, its virulence potential remains to be investigated. in the present study, the immune responses of human macrophages and gingival fibroblasts upon stimulation with wild-type t. forsythia and an s-layer-deficient mutant were investigated. the mrna expression levels of the pro-inflammatory mediators ... | 2010 | 20929722 |
| potential of the tannerella forsythia s-layer to delay the immune response. | the periodontal pathogen tannerella forsythia possesses a glycosylated s-layer as an outermost cell decoration. while the s-layer provides a selection advantage to the bacterium in the natural habitat, its virulence potential remains to be investigated. in the present study, the immune responses of human macrophages and gingival fibroblasts upon stimulation with wild-type t. forsythia and an s-layer-deficient mutant were investigated. the mrna expression levels of the pro-inflammatory mediators ... | 2010 | 20929722 |
| virulence factors of the oral spirochete treponema denticola. | there is compelling evidence that treponemes are involved in the etiology of several chronic diseases, including chronic periodontitis as well as other forms of periodontal disease. there are interesting parallels with other chronic diseases caused by treponemes that may indicate similar virulence characteristics. chronic periodontitis is a polymicrobial disease, and recent animal studies indicate that co-infection of treponema denticola with other periodontal pathogens can enhance alveolar bone ... | 2010 | 20940357 |
| real-time polymerase chain reaction of "red complex" (porphyromonas gingivalis, tannerella forsythia, and treponema denticola) in periradicular abscesses. | the red complex bacteria (porphyromonas gingivalis, tannerella forsythia, and treponema denticola) have been implicated, either singly or in combination, in the development of various forms of periodontal diseases. the aim of this study was to investigate the presence of "red complex" in acute periradicular abscesses by real-time polymerase chain reaction (pcr) method. | 2010 | 20955954 |
| hemagglutinin/adhesin domains of porphyromonas gingivalis play key roles in coaggregation with treponema denticola. | porphyromonas gingivalis and treponema denticola are major pathogens of periodontal disease. coaggregation between microorganisms plays a key role in the colonization of the gingival crevice and the organization of periodontopathic biofilms. we investigated the involvement of surface ligands of p. gingivalis in coaggregation. two triple mutants of p. gingivalis lacking arg-gingipain a (rgpa), lys-gingipain (kgp) and hemagglutinin a (haga) or rgpa, arg-gingipain b (rgpb) and kgp showed significan ... | 2010 | 21039921 |
| characterization of a novel family of fibronectin-binding proteins with m23 peptidase domains from treponema denticola. | interactions with fibronectin are important in the virulence strategies of a range of disease-related bacteria. the periodontitis-associated oral spirochaete treponema denticola expresses at least two fibronectin-binding proteins, designated msp (major surface protein) and oppa (oligopeptide-binding protein homologue). to identify other t. denticola outer membrane fibronectin-binding proteins, the amino acid sequence of the treponema pallidum fibronectin-binding protein tp0155 was used to survey ... | 2010 | 21040511 |
| transcriptional profiles of treponema denticola in response to environmental conditions. | the periodontal pathogen t. denticola resides in a stressful environment rife with challenges, the human oral cavity. knowledge of the stress response capabilities of this invasive spirochete is currently very limited. whole genome expression profiles in response to different suspected stresses including heat shock, osmotic downshift, oxygen and blood exposure were examined. most of the genes predicted to encode conserved heat shock proteins (hsps) were found to be induced under heat and oxygen ... | 2010 | 21048920 |
| early bacterial colonization and soft tissue health around zirconia and titanium abutments: an in vivo study in man. | aim: to compare the early bacterial colonization and soft tissue health of mucosa adjacent to zirconia (zro(2) ) and titanium (ti) abutment surfaces in vivo. materials and methods: twenty edentulous subjects received two endosseous mandibular implants. the implants were fitted with either a zro(2) or a ti abutment (non-submerged implant placement, within-subject comparison, left-right randomization). sulcular bacterial sampling and the assessment of probing pocket depth, recession and bleeding o ... | 2010 | 21054554 |
| characterization of novel bovine gastrointestinal tract treponema isolates and comparison with bovine digital dermatitis treponemes. | this study aimed to isolate and characterize treponemes present in the bovine gastrointestinal (gi) tract and compare them with bovine digital dermatitis (bdd) treponemes. seven spirochete isolates were obtained from the bovine gi tract, which, on the basis of 16s rrna gene comparisons, clustered within the genus treponema as four novel phylotypes. one phylotype was isolated from several different gi tract regions, including the omasum, colon, rumen, and rectum. these four phylotypes could be di ... | 2010 | 21057019 |
| characterization of novel bovine gastrointestinal tract treponema isolates and comparison with bovine digital dermatitis treponemes. | this study aimed to isolate and characterize treponemes present in the bovine gastrointestinal (gi) tract and compare them with bovine digital dermatitis (bdd) treponemes. seven spirochete isolates were obtained from the bovine gi tract, which, on the basis of 16s rrna gene comparisons, clustered within the genus treponema as four novel phylotypes. one phylotype was isolated from several different gi tract regions, including the omasum, colon, rumen, and rectum. these four phylotypes could be di ... | 2010 | 21057019 |
| targeting selenium metabolism and selenoproteins: novel avenues for drug discovery. | selenoproteins play a wide range of roles in metabolism and oxidative stress defense and are produced by organisms in all three domains of life. recent evidence has been presented that metal based cancer drugs target the selenol nucleophile of the active site selenocysteine in thioredoxin reductase isoenzymes. other metals and metalloids, such as tin, arsenic and gold, have also recently been shown to form stable complexes with hydrogen selenide, a required precursor for the synthesis of selenop ... | 2010 | 21069141 |
| systemic inflammatory responses in progressing periodontitis during pregnancy in a baboon model. | this study tested the hypothesis that pregnant female baboons exhibit increased levels of various inflammatory mediators in serum resulting from ligature-induced periodontitis, and that these profiles would relate to periodontal disease severity/extent in the animals. the animals were sampled at baseline (b), mid-pregnancy (mp; two quadrants ligated) and at delivery (d; four quadrants ligated). all baboons developed increased plaque, gingival inflammation and bleeding, pocket depths and attachme ... | 2010 | 21070210 |
| polybacterial challenge enhances hiv reactivation in latently infected macrophages and dendritic cells. | a polymicrobial infection comprising subgingival biofilms is the trigger for the chronic immunoinflammatory lesions of periodontitis. these microbial biofilms interface with host immune cells that increase with progressing disease and could result in hiv reactivation in hiv-1-infected patients. previous reports have focused on the ability of monospecies challenge of macrophages and dendritic cells to detail molecular aspects of their detection and signalling pathways. this study provides a semin ... | 2010 | 21073452 |
| microbiota of deciduous endodontic infections analysed by mda and checkerboard dna-dna hybridization. | to evaluate the microbiota of endodontic infections in deciduous teeth by checkerboard dna-dna hybridization after uniform amplification of dna in samples by multiple displacement amplification (mda). | 2010 | 21083570 |
| microbiota of deciduous endodontic infections analysed by mda and checkerboard dna-dna hybridization. | to evaluate the microbiota of endodontic infections in deciduous teeth by checkerboard dna-dna hybridization after uniform amplification of dna in samples by multiple displacement amplification (mda). | 2010 | 21083570 |
| the effect of a single episode of antimicrobial photodynamic therapy in the treatment of experimental periodontitis. microbiological profile and cytokine pattern in the dog mandible. | the purpose of this study was to evaluate the effect of a single application of antimicrobial photodynamic therapy (apdt) on microbiological profile and cytokine pattern in dogs. periodontal disease was induced by placing 3.0 silk ligatures around the mandibular pre-molars bilaterally during 8 weeks. the dogs were randomly treated with apdt using a dye/laser system, scaling and root planning (srp), or with the association of treatments (srp + apdt). plaque samples were collected at baseline, 1, ... | 2010 | 21086009 |
| integrating '-omics' and natural product discovery platforms to investigate metabolic exchange in microbiomes. | the microbiome is an abundance of microorganisms within a host (e.g. human microbiome). these microorganisms produce small molecules and metabolites that have been shown to affect and dictate the physiology of an individual. functional knowledge of these molecules, often produced for communication or defense, will reveal the interplay between microbes and host in health and disease. the vast diversity in structure and function of microbiome-associated small molecules necessitate tools that will ... | 2010 | 21087892 |
| integrating '-omics' and natural product discovery platforms to investigate metabolic exchange in microbiomes. | the microbiome is an abundance of microorganisms within a host (e.g. human microbiome). these microorganisms produce small molecules and metabolites that have been shown to affect and dictate the physiology of an individual. functional knowledge of these molecules, often produced for communication or defense, will reveal the interplay between microbes and host in health and disease. the vast diversity in structure and function of microbiome-associated small molecules necessitate tools that will ... | 2010 | 21087892 |
| one-stage full-mouth disinfection as a therapeutic approach for generalized aggressive periodontitis. | background: data concerning treatment outcomes in patients with generalized aggressive periodontitis (gagp) are limited. the aim of this study is to investigate 6-month clinical and microbiologic outcomes of the one-stage full-mouth disinfection (osfmd) in the management of patients with gagp. methods: twenty-seven patients with advanced gagp were included in this prospective follow-up intervention study. clinical and microbiologic parameters were collected at baseline and 3 and 6 months after t ... | 2010 | 21091345 |
| periodontal pathogens interfere with quorum-sensing-dependent virulence properties in streptococcus mutans. | the mechanism by which periodontal pathogens dominate at disease sites is not yet understood. one possibility is that these late colonizers antagonize the quorum-sensing systems of early colonizers and render those early colonizers less resistant to environmental factors. in this study, we utilized streptococcus mutans, a well-documented oral streptococcus with many quorum-sensing-dependent properties, as an example of an earlier colonizer antagonized by periodontal pathogens. | 2010 | 21108642 |
| the treponema denticola chymotrypsin-like protease dentilisin induces matrix metalloproteinase-2-dependent fibronectin fragmentation in periodontal ligament cells. | periodontal disease is a bacterially mediated chronic inflammatory disease that results in destruction of the periodontal ligament (pdl) and alveolar bone that surround and support the dentition. while their precise roles are not well understood, periodontal pathogens, including treponema denticola, are believed to initiate the destructive inflammatory responses and dysregulation of tissue homeostasis that characterize the disease. these responses are believed to result from both proinflammatory ... | 2010 | 21115719 |
| antialarmin effect of tick saliva during the transmission of lyme disease. | tick saliva has potent immunomodulatory properties. in arthropod-borne diseases, this effect is largely used by microorganisms to increase their pathogenicity and to evade host immune responses. we show that in lyme borreliosis, tick salivary gland extract and a tick saliva protein, salp15, inhibit in vitro keratinocyte inflammation induced by borrelia burgdorferi sensu stricto or by the major outer surface lipoprotein of borrelia, ospc. chemokines (interleukin-8 [il-8] and monocyte chemoattract ... | 2010 | 21134970 |
| antialarmin effect of tick saliva during the transmission of lyme disease. | tick saliva has potent immunomodulatory properties. in arthropod-borne diseases, this effect is largely used by microorganisms to increase their pathogenicity and to evade host immune responses. we show that in lyme borreliosis, tick salivary gland extract and a tick saliva protein, salp15, inhibit in vitro keratinocyte inflammation induced by borrelia burgdorferi sensu stricto or by the major outer surface lipoprotein of borrelia, ospc. chemokines (interleukin-8 [il-8] and monocyte chemoattract ... | 2010 | 21134970 |
| the central region of the msp gene of treponema denticola has sequence heterogeneity among clinical samples, obtained from patients with periodontitis. | treponema denticola is an oral spirochete involved in the pathogenesis and progression of periodontal disease. of its virulence factors, the major surface protein (msp) plays a role in the interaction between the treponeme and host. to understand the possible evolution of this protein, we analyzed the sequence of the msp gene in 17 t. denticola positive clinical samples. | 2010 | 21138575 |
| community signalling between streptococcus gordonii and porphyromonas gingivalis is controlled by the transcriptional regulator cdhr. | interspecies signalling between porphyromonas gingivalis and streptococcus gordonii serves to constrain development of dual species communities. contact with s. gordonii propagates a tyrosine phosphorylation-dependent signal within p. gingivalis that culminates in reduced transcription of adhesin and signalling genes. here we demonstrate the involvement of the p. gingivalis orphan luxr family transcription factor pgn_1373, which we designate cdhr, in this control pathway. expression of cdhr is e ... | 2010 | 21143321 |
| confounding and interaction effect of treponema denticola salivary carriage in chronic periodontitis. | to evaluate the salivary carriage of treponema denticola and its association with demographic variables in the etiopathogenesis of chronic periodontitis. | 2010 | 19849805 |
| global challenge of antibiotic-resistant treponema pallidum. | syphilis is a multistage infectious disease that is usually transmitted through contact with active lesions of a sexual partner or from an infected pregnant woman to her fetus. despite elimination efforts, syphilis remains endemic in many developing countries and has reemerged in several developed countries, including china, where a widespread epidemic recently occurred. in the absence of a vaccine, syphilis control is largely dependent upon identification of infected individuals and treatment o ... | 2010 | 19805553 |
| global challenge of antibiotic-resistant treponema pallidum. | syphilis is a multistage infectious disease that is usually transmitted through contact with active lesions of a sexual partner or from an infected pregnant woman to her fetus. despite elimination efforts, syphilis remains endemic in many developing countries and has reemerged in several developed countries, including china, where a widespread epidemic recently occurred. in the absence of a vaccine, syphilis control is largely dependent upon identification of infected individuals and treatment o ... | 2010 | 19805553 |
| quantification of periodontal pathogens by paper point sampling from the coronal and apical aspect of periodontal lesions by real-time pcr. | the present study compared the recovery of six periodontal pathogens by paper point samples from two different aspects of periodontal lesions by quantitative real-time polymerase chain reaction (pcr). twenty patients with untreated chronic periodontitis were randomized into two groups. before subgingival instrumentation and after 10 weeks samples in group a were taken first with a paper point half length (hp) of the probing depth, then with a paper point full length (fp) at the same site. in gro ... | 2010 | 19693550 |
| a novel murine model for chronic inflammatory alveolar bone loss. | chronic inflammatory bowel disease (ibd) demonstrates some similarities to the dysregulated chronic immunoinflammatory lesion of periodontitis. trinitrobenzene sulphonic acid (tnbs) and dextran sodium sulphate (dss) administered to rodents have been shown to elicit inflammatory responses that undermine the integrity of the gut epithelium in a similar manner to ibd in humans. the objective of this study was to evaluate the ability of these chemicals to elicit periodontal inflammation as a novel m ... | 2010 | 19602109 |
| a novel murine model for chronic inflammatory alveolar bone loss. | chronic inflammatory bowel disease (ibd) demonstrates some similarities to the dysregulated chronic immunoinflammatory lesion of periodontitis. trinitrobenzene sulphonic acid (tnbs) and dextran sodium sulphate (dss) administered to rodents have been shown to elicit inflammatory responses that undermine the integrity of the gut epithelium in a similar manner to ibd in humans. the objective of this study was to evaluate the ability of these chemicals to elicit periodontal inflammation as a novel m ... | 2010 | 19602109 |
| hydrogen sulfide induces apoptosis in human periodontium cells. | the existence of hydrogen sulfide (h(2)s) at high concentrations in periodontal pockets is a characteristic feature of periodontitis. periodontal pathogens play a key role in the production of h(2)s under these etiology conditions. this study was designed to examine the cytotoxicity of h(2)s in periodontium cells, including human periodontal ligament (pdl) cells and human gingival fibroblasts (hgfs), as well as the role of h(2)s in apoptosis induction. | 2010 | 19602114 |
| complement control protein factor h: the good, the bad, and the inadequate. | the complement system is an essential component of the innate immune system that participates in elimination of pathogens and altered host cells and comprises an essential link between the innate and adaptive immune system. soluble and membrane-bound complement regulators protect cells and tissues from unintended complement-mediated injury. complement factor h is a soluble complement regulator essential for controlling the alternative pathway in blood and on cell surfaces. normal recognition of ... | 2010 | 20580090 |
| electron cryotomography. | electron cryotomography (ect) is an emerging technology that allows thin samples such as macromolecular complexes and small bacterial cells to be imaged in 3-d in a nearly native state to "molecular" ( approximately 4 nm) resolution. as such, ect is beginning to deliver long-awaited insight into the positions and structures of cytoskeletal fi laments, cell wall elements, motility machines, chemoreceptor arrays, internal compartments, and other ultrastructures. this article describes the techniqu ... | 2010 | 20516135 |
| detection rates of presumptive periodontal pathogens in subgingival plaque samples of untreated periodontitis using either four or six pooled samples. | a comparison of the detection frequency and number of periodontal pathogens in patients with aggressive or generalized, severe chronic periodontitis using a gene-probe analysis. | 2010 | 25427269 |
| the effect of locally delivered doxycycline in the treatment of chronic periodontitis. a clinical and microbiological cohort study. | to evaluate the efficacy of locally delivered doxycycline as an adjunct to non-surgical treatment with the use of an ultrasonic device compared to scaling and root planing using hand instruments, by means of clinical and microbiological criteria. | 2010 | 24421978 |
| the effect of locally delivered doxycycline in the treatment of chronic periodontitis. a clinical and microbiological cohort study. | to evaluate the efficacy of locally delivered doxycycline as an adjunct to non-surgical treatment with the use of an ultrasonic device compared to scaling and root planing using hand instruments, by means of clinical and microbiological criteria. | 2010 | 24421978 |
| cardiovascular disease and the role of oral bacteria. | in terms of the pathogenesis of cardiovascular disease (cvd) the focus has traditionally been on dyslipidemia. over the decades our understanding of the pathogenesis of cvd has increased, and infections, including those caused by oral bacteria, are more likely involved in cvd progression than previously thought. while many studies have now shown an association between periodontal disease and cvd, the mechanisms underpinning this relationship remain unclear. this review gives a brief overview of ... | 2010 | 21523220 |
| diabetes, periodontitis, and the subgingival microbiota. | both type 1 and type 2 diabetes have been associated with increased severity of periodontal disease for many years. more recently, the impact of periodontal disease on glycaemic control has been investigated. the role of the oral microbiota in this two-way relationship is at this stage unknown. further studies, of a longitudinal nature and investigating a wider array of bacterial species, are required in order to conclusively determine if there is a difference in the oral microbiota of diabetics ... | 2010 | 21523215 |
| microbiota associated with chronic osteomyelitis of the jaws. | chronic osteomyelitis of maxilla and mandible is rare in industrialized countries and its occurrence in developing countries is associated with trauma and surgery, and its microbial etiology has not been studied thoroughly. the aim of this investigation was to evaluate the microbiota associated with osteomyelitis of mandible or maxilla from some brazilian patients. after clinical and radiographic evaluation, samples of bone sequestra, purulent secretion, and biopsies of granulomatous tissues fro ... | 2010 | 24031586 |
| non-surgical instrumentation associated with povidone-iodine in the treatment of interproximal furcation involvements. | the aim of this controlled clinical trial was to evaluate the effect of topically applied povidone-iodine (pvp-i) used as an adjunct to non-surgical treatment of interproximal class ii furcation involvements. | 2010 | 21308291 |
| evolutionary divergence of chloroplast fad synthetase proteins. | flavin adenine dinucleotide synthetases (fadss) - a group of bifunctional enzymes that carry out the dual functions of riboflavin phosphorylation to produce flavin mononucleotide (fmn) and its subsequent adenylation to generate fad in most prokaryotes - were studied in plants in terms of sequence, structure and evolutionary history. | 2010 | 20955574 |
| endodontic microbiology. | root canal therapy has been practiced ever since 1928 and the success rate has tremendously increased over the years owing to various advancements in the field. one main reason is the complete understanding of the microbiology involved in the endodontic pathology. this has helped us to modify the conventional treatment plans and effectively combat the microorganisms. now, studies are aiming to explore the characteristics of the "most" resistant organism and the methods to eliminate them. this ar ... | 2010 | 21217951 |
| a nested leucine rich repeat (lrr) domain: the precursor of lrrs is a ten or eleven residue motif. | leucine rich repeats (lrrs) are present in over 60,000 proteins that have been identified in viruses, bacteria, archae, and eukaryotes. all known structures of repeated lrrs adopt an arc shape. most lrrs are 20-30 residues long. all lrrs contain lxxlxlxxnxl, in which "l" is leu, ile, val, or phe and "n" is asn, thr, ser, or cys and "x" is any amino acid. seven classes of lrrs have been identified. however, other lrr classes remains to be characterized. the evolution of lrrs is not well understoo ... | 2010 | 20825685 |
| oral microbiota and cancer. | inflammation caused by infections may be the most important preventable cause of cancer in general. however, in the oral cavity the role of microbiota in carcinogenesis is not known. microbial populations on mouth mucosa differ between healthy and malignant sites and certain oral bacterial species have been linked with malignancies but the evidence is still weak in this respect. nevertheless, oral microorganisms inevitably up-regulate cytokines and other inflammatory mediators that affect the co ... | 2010 | 21523227 |
| azithromycin decreases crevicular fluid volume and mediator content. | azithromycin enhances the response to root planing and produces anti-inflammatory effects in treating chronic lung disease. this led us to hypothesize that azithromycin inhibits inflammatory mediator production in gingiva, leading to decreased gingival crevicular fluid (gcf) volume. to test this hypothesis, ten periodontally healthy volunteers received azithromycin every 24 hours for 48 hours. gcf samples were collected from 12 maxillary interproximal sites prior to azithromycin (baseline) and 2 ... | 2010 | 20400725 |
| periodontal bacteria and hypertension: the oral infections and vascular disease epidemiology study (invest). | chronic infections, including periodontal infections, may predispose to cardiovascular disease. we investigated the relationship between periodontal microbiota and hypertension. | 2010 | 20453665 |
| oral health and frailty in the medieval english cemetery of st mary graces. | the analysis of oral pathologies is routinely a part of bioarcheological and paleopathological investigations. oral health, while certainly interesting by itself, is also potentially informative about general or systemic health. numerous studies within modern populations have shown associations between oral pathologies and other diseases, such as cardiovascular disease, certain types of cancer, and pulmonary infections. this article addresses the question of how oral health was associated with g ... | 2010 | 19927365 |
| self-targeting by crispr: gene regulation or autoimmunity? | the recently discovered prokaryotic immune system known as crispr (clustered regularly interspaced short palindromic repeats) is based on small rnas ('spacers') that restrict phage and plasmid infection. it has been hypothesized that crisprs can also regulate self gene expression by utilizing spacers that target self genes. by analyzing crisprs from 330 organisms we found that one in every 250 spacers is self-targeting, and that such self-targeting occurs in 18% of all crispr-bearing organisms. ... | 2010 | 20598393 |
| alveolar osteitis: a comprehensive review of concepts and controversies. | alveolar osteitis, "dry socket", remains amongst the most commonly encountered complications following extraction of teeth by general dentists and specialists. a great body of literature is devoted to alveolar osteitis addressing the etiology and pathophysiology of this condition. in addition numerous studies are available discussing methods and techniques to prevent this condition. to this date though great controversy still exists regarding the appropriate terminology used for this condition a ... | 2010 | 20652078 |
| comparative evaluation of ifnγ, il4 and il17 cytokines in healthy gingiva and moderate to advanced chronic periodontitis. | considering the role of t cells in the pathogenesis of periodontitis, the purpose of this study was to compare the amount of ifnγ, il4 and il17 in advanced periodontal lesions with healthy gum areas to determine each th1, th2 and th17 cells activity in comparison with each other and finally, to compare the value and the role of humoral, cell mediate and autoimmune responses. | 2010 | 22013456 |
| association of periodontitis with rheumatoid arthritis and atherosclerosis: novel paradigms in etiopathogeneses and management? | there is increasing documentation of a link between inflammatory periodontal disease affecting the supporting structure of teeth, rheumatoid arthritis, and coronary artery disease. periodontitis is initiated predominantly by gram-negative bacteria and progresses as a consequence of the host inflammatory response to periodontal pathogens. lipopolysaccharide, a cell wall constituent stimulates the production of inflammatory cytokines via the activation of signaling pathways perpetuating inflammato ... | 2010 | 27789992 |
| the distributed genome hypothesis as a rubric for understanding evolution in situ during chronic bacterial biofilm infectious processes. | most chronic infectious disease processes associated with bacteria are characterized by the formation of a biofilm that provides for bacterial attachment to the host tissue or the implanted medical device. the biofilm protects the bacteria from the host's adaptive immune response as well as predation by phagocytic cells. however, the most insidious aspect of biofilm biology from the host's point of view is that the biofilm provides an ideal setting for bacterial horizontal gene transfer (hgt). h ... | 2010 | 20618850 |
| characteristics of inflammation common to both diabetes and periodontitis: are predictive diagnosis and targeted preventive measures possible? | diabetes and periodontitis are chronic inflammatory disorders that contribute to each others' severity and worsen each others' prognosis. studies have shown that patients with diabetes are at increased risk of developing periodontitis, and that diabetics with untreated periodontitis have more difficulty controlling serum glucose. periodontal treatment that reduces gingival inflammation aids in the control of hyperglycemia. periodontitis is accompanied by gingival bleeding and the production of a ... | 2010 | 23199045 |
| relationships between subgingival microbiota and gcf biomarkers in generalized aggressive periodontitis. | to examine relationships between subgingival biofilm composition and levels of gingival crevicular fluid (gcf) cytokines in periodontal health and generalized aggressive periodontitis (gap). | 2010 | 20447254 |
| abundant oligonucleotides common to most bacteria. | bacteria show a bias in their genomic oligonucleotide composition far beyond that dictated by g+c content. patterns of over- and underrepresented oligonucleotides carry a phylogenetic signal and are thus diagnostic for individual species. patterns of short oligomers have been investigated by multiple groups in large numbers of bacteria genomes. however, global distributions of the most highly overrepresented mid-sized oligomers have not been assessed across all prokaryotes to date. we surveyed o ... | 2010 | 20352124 |
| subgingival biofilm formation. | 2010 | 20017794 | |
| bacterial invasion of epithelial cells and spreading in periodontal tissue. | 2010 | 20017796 | |
| buccal alterations in diabetes mellitus. | long standing hyperglycaemia besides damaging the kidneys, eyes, nerves, blood vessels, heart, can also impair the function of the salivary glands leading to a reduction in the salivary flow. when salivary flow decreases, as a consequence of an acute hyperglycaemia, many buccal or oral alterations can occur such as: a) increased concentration of mucin and glucose; b) impaired production and/or action of many antimicrobial factors; c) absence of a metalloprotein called gustin, that contains zinc ... | 2010 | 20180965 |
| relationships among gingival crevicular fluid biomarkers, clinical parameters of periodontal disease, and the subgingival microbiota. | the objectives of this study were to measure levels of gingival crevicular fluid (gcf) biomarkers and subgingival bacterial species in periodontally healthy subjects and subjects with periodontitis to explore the relationships among these biomarkers, the subgingival microbiota, and the clinical parameters of periodontal disease. | 2010 | 20059421 |
| relationships among interleukin-6, tumor necrosis factor-α, adipokines, vitamin d, and chronic periodontitis. | the aim of this study is to explore relationships among serum adipokines, vitamin d, and clinical and microbial parameters of chronic periodontitis before and after treatment. | 2011 | 22181684 |
| relationships among interleukin-6, tumor necrosis factor-α, adipokines, vitamin d, and chronic periodontitis. | the aim of this study is to explore relationships among serum adipokines, vitamin d, and clinical and microbial parameters of chronic periodontitis before and after treatment. | 2011 | 22181684 |
| bacterial biogeography of the human digestive tract. | we present bacterial biogeography as sampled from the human gastrointestinal tract of four healthy subjects. this study generated >32 million paired-end sequences of bacterial 16s rrna genes (v3 region) representing >95,000 unique operational taxonomic units (otus; 97% similarity clusters), with >99% good's coverage for all samples. the highest otu richness and phylogenetic diversity was found in the mouth samples. the microbial communities of multiple biopsy sites within the colon were highly s ... | 2011 | 22355685 |
| neutrophils in periodontal inflammation. | neutrophils (also called polymorphonuclear leukocytes) are the most abundant leukocytes whose primary purpose as anti-microbial professional phagocytes is to kill extracellular pathogens. neutrophils and macrophages are phagocytic cell types that along with other cells effectively link the innate and adaptive arms of the immune response, and help promote inflammatory resolution and tissue healing. found extensively within the gingival crevice and epithelium, neutrophils are considered the key pr ... | 2011 | 22142957 |
| neutrophils in periodontal inflammation. | neutrophils (also called polymorphonuclear leukocytes) are the most abundant leukocytes whose primary purpose as anti-microbial professional phagocytes is to kill extracellular pathogens. neutrophils and macrophages are phagocytic cell types that along with other cells effectively link the innate and adaptive arms of the immune response, and help promote inflammatory resolution and tissue healing. found extensively within the gingival crevice and epithelium, neutrophils are considered the key pr ... | 2011 | 22142957 |
| the influence of micronutrients on oral and general health. | the aim of the present clinical pilot study was to examine the influence of a combination of micronutrients on individuals with high stress experience. | 2011 | 22027646 |
| stick to your gums: mechanisms of oral microbial adherence. | studies on the adherence properties of oral bacteria have been a major focus in microbiology research for several decades. the ability of bacteria to adhere to the variety of surfaces present in the oral cavity, and to become integrated within the resident microbial communities, confers growth and survival properties. molecular analyses have revealed several families of gram-positive bacterial surface proteins, including serine-rich repeat, antigen i/ii, and pilus families, that mediate adherenc ... | 2011 | 21335541 |
| comparative genomics evidence that only protein toxins are tagging bad bugs. | the term toxin was introduced by roux and yersin and describes macromolecular substances that, when produced during infection or when introduced parenterally or orally, cause an impairment of physiological functions that lead to disease or to the death of the infected organism. long after the discovery of toxins, early genetic studies on bacterial virulence demonstrated that removing a certain number of genes from pathogenic bacteria decreases their capacity to infect hosts. each of the removed ... | 2011 | 22919573 |