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occurrence of human papillomavirus infection in cervical intraepithelial neoplasia. a retrospective histopathological study of 317 cases treated by laser conization.the aim of the study was to compare the histological outcome of the cone specimens with the diagnoses of the preoperative biopsies, to assess the distribution of histological features consistent with human papillomavirus (hpv) infection and, finally, to analyse the impact of cellular hpv features on classification of cervical intraepithelial neoplasia (cin). the study comprised a population of 317 women treated for cin by laser conization during the period 1983-85. a total of 634 cervical specim ...19958534427
competition inhibition of cytotoxic t-lymphocyte (ctl) lysis, a more sensitive method to identify candidate ctl epitopes than induction of antibody-detected mhc class i stabilization.we compared the efficiency of two commonly used cellular major histocompatibility complex (mhc) class i peptide-binding assays to identify a cytotoxic t lymphocyte (ctl) epitope-containing peptide among length variants derived from the human papilloma virus type 16 (hpv 16) oncoprotein e7. although both assays identified the same sequence (e7 49-57) as the most efficient db-binding peptide, the efficiency by which they did so differed markedly. in a peptide competition cytotoxicity (pcc) assay, ...19958537084
human papillomavirus-associated malignancies in hiv-positive men and women.human papillomavirus (hpv) infections of the anogenital tract and hpv-associated anogenital neoplasia are commonly found in hiv-positive men and women. although there is little evidence suggesting that the incidence of invasive cervical cancer is increased in hiv-positive women, the incidence of invasive anal cancer may be increased in hiv-positive men. among hiv-positive women, cervical intraepithelial neoplasia progresses more rapidly and recurs more often after primary therapy than in hiv-neg ...19958541389
pathobiology of human papillomavirus.human papillomavirus (hpv) has long been recognized as the etiologic agent of skin and genital warts, and only recently has an oncogenic role been attributed to the virus. this article discusses the classification, genetics, and pathogenesis of hpv. hpv detection, epidemiology of hpv and cervical neoplasia, and hpv infections in the genital tract are also reviewed.19958542729
detection of human papillomavirus in juvenile laryngeal papillomatosis using polymerase chain reaction.we examined the presence and subtypes of human papillomavirus (hpv) in 20 paraffin-embedded samples (from 12 patients) of juvenile laryngeal papillomatosis using the polymerase chain reaction (pcr). the biopsies had been stored for months to 12 years. due to the great genetic variability of hpv, we selected a conservative sequence of the viral genome (l1 region) to identify the vast majority of the subtypes. positive results were obtained by one-step pcr amplification with the my09-11 consensus ...19958544718
role of human papillomavirus and p53 tumor suppressor gene in cervical carcinogenesis.to determine whether the dysfunction of p53 protein, caused either by the mutation of p53 gene itself or by the human papillomavirus (hpvs) is involved in the development of cervical cancers and to find out the status of p53 tumor suppressor gene in hpv positive or negative cancers, we analyzed 64 cases of primary cervix cancers. first, polymerase chain reaction (pcr) was performed with e6 consensus primer pairs to detect the infection of hpvs in cervix cancer tissues. second, to screen the p53 ...19958546000
p53 protein accumulation and the presence of human papillomavirus dna in bronchiolo-alveolar carcinoma correlate with poor prognosis.accumulation of the tumour suppressor gene p53 product due to a gene mutation is frequently seen in human carcinomas, including lung carcinoma. another indirect mechanism involving p53 in malignant growth relates to the e6 protein of the human papillomavirus (hpv), which is able to bind and degrade wild-type p53 protein, thus eliminating its tumour suppressor activities. bronchiolo-alveolar carcinoma (bac) is a rare type of lung carcinoma. the aim of our study was to examine the occurrence of p5 ...19958550245
effectiveness of three ribozymes for cleavage of an rna transcript from human papillomavirus type 18.we tested three hammerhead ribozymes for their ability to bind and cleave rna transcripts derived from the e6 and e7 genes of human papillomavirus (hpv) type-18. targets were located at nucleotides (nt) 123, 309, and 671 of the viral transcript. in vitro each ribozyme hybridized to its target site when the ribozyme:target ratio was 20:1 or greater and achieved maximal hybridization within 1 hour. hpv rna from the hela cervical cancer cell line was cleaved effectively by each ribozyme. when hpv r ...19958548580
cellular immunity against dna tumour viruses: possibilities for peptide-based vaccines and immune escape. 19958566445
intermethod variation in detection of human papillomavirus dna in cervical smears.in order to investigate the reliability of detection of human papillomavirus (hpv) dna in cervical smears, we have compared the performance of two hpv pcr systems, the cpi/iig and my09/11 primer-mediated pcrs and the hybrid capture system hpv dna detection test (hybrid capture assay), in detecting hpv dna in cervical smears. we also included in our study the my09/11b pcr plus sharp (solution hybridization assay for pcr products) signal system. this sharp signal system was recently developed to d ...19958567896
basaloid carcinoma and keratinizing squamous cell carcinoma of the vulva: a case of two primary carcinomas.basaloid carcinomas of the vulva have been reported to occur mostly in young women and are often associated with human papillomavirus (hpv) infection, whereas older women mostly suffer from keratinizing squamous cell carcinomas unassociated with hpv. the rare case of a 75 year old woman coincidentally suffering from a basaloid carcinoma and a keratinizing squamous cell carcinoma of the vulva is reported. with in situ hybridisation, hybrid capture and immunohistochemical methods we searched for h ...19958572644
dendritic cells as carriers for a cytotoxic t-lymphocyte epitope-based peptide vaccine in protection against a human papillomavirus type 16-induced tumor.previously we have demonstrated that two doses of a cytotoxic t lymphocyte (ctl) epitope-based peptide vaccine of human papillomavirus type 16 (hpv 16) e7 aa 49-57 elicit protection against outgrowth of hpv 16-transformed tumor cells (c3 cells) in b6 mice. incomplete freund's adjuvant (ifa), as a carrier, was used to induce this response. to avoid side effects caused by the use of external adjuvants, we have now investigated the effectiveness of highly purified spleen dendritic cells (dc) that e ...19958574470
urethral condylomata, due to human papilloma virus (hpv) type 6/11., associated with transitional cell tumors in the bladder and ureter. a case report.condylomata in the urethra usually occur as a complication to genital warts on the external genitals and are most often situated in the distal part of the urethra. this is a report of a male patient with recurring papillomatous transitional cell tumors, stage ta and t1, grade 2 and 3 in the bladder and the right ureter, who presented with extensive condylomatosis in the urethra from the meatus to the verumontanum, without simultaneous presence of condylomata at the external genitals. condylomato ...19958578257
[current concepts on human papillomavirus infection].the human papilloma virus presents a multidimensional problem for gynecologist and urologist. the human papilloma virus infection (hpvi) incidence has been increased, so that at present it's the viral infection most common of the genital tract. the hpv is transmitted for sexual contact but it has not been explained the infection mechanism of the virus-cell interaction of the host. the long term consequences of the sexual transmission diseases are more serious in female than in male. the majority ...19958586297
vaccines in the treatment of cancer.the development of vaccines for treating cancer is discussed. the central hypothesis behind active specific immunotherapy for cancer is that tumor cells express unique antigens that tell the immune system that something about these cells is foreign. a vaccine is a way of delivering an antigen to the immune system such that immune cells recognize the antigen as foreign and destroy any cells bearing that antigen. early trials of vaccines for treating cancer were limited by technical problems relat ...19958590245
[detection and typing of hpv dna in situ--improvement in diagnosis of lesions of the uterine cervix].we examined the cervical samples from 63 female patients with various grades of cervical intraepithelial neoplasia (cin), invasive squamous cell carcinoma (inca) or inflammation. all the women with the diagnosis of cin were selected on the basis of cytological prediction of hpv infection (koilocytosis, dyskeratosis). the analysis of human papillomavirus (hpv) genome by dna hybridization in situ was done in all cases. simultaneously, the immunohistochemical expression of papillomavirus common ant ...19958599698
p53 mutation and mdm2 amplification are rare even in human papillomavirus-negative cervical carcinomas.mutation of the p53 tumor suppressor gene is the most commonly found genetic alteration in human cancer. the e6 gene product of human papillomavirus (hpv) 16 and 18 can inactivate the p53 protein by promoting its degradation. because most hpv-positive cervical carcinoma cell lines contain wild-type p53 whereas hpv-negative cell lines have point mutations in the p53 gene, a major role in the development of hpv-negative cervical cancer has been attributed to p53. recent studies, however, have obse ...19958630877
papillomavirus, p53 alteration, and primary carcinoma of the vulva.twenty-nine samples from 28 cases of vulvar squamous cell carcinoma, of which 13 fulfilled the criteria of the bowenoid subtype (mean age 45 years, range 31-68) and 16 of the usual subtype of invasive squamous cell carcinoma (iscc) (mean age 67.5 years, range 34-83) were investigated for human papillomavirus (hpv) dna, tp53 alterations, and mdm2 and bcl-2 gene product deregulation. microscopically all the bowenoid subtype cases (group i) showed a high-grade intraepithelial (vin 3, carcinoma in s ...19958634779
impact of the bethesda system.although the bethesda system provides concise, specific descriptions for classifying cervical cytology, its usefulness has been questioned in the united states and abroad. the author attempted to evaluate the impact of the bethesda system on cervical screening and subsequent management decisions.19958634982
detection of human papillomavirus dna sequences in oral squamous cell carcinomas and their relation to p53 and proliferating cell nuclear antigen expression.the etiology of oral squamous cell carcinoma (scc) is still obscure. since human papillomavirus (hpv) dnas are associated with carcinoma of the uterine cervix, carcinomas of the oral cavity were investigated to ascertain if these viruses are present in squamous carcinomas of this anatomic site.19958635051
antibodies prevalence against hpv-6b and -16 recombinant fusion proteins in korean patients with cervical neoplasia.this study was to investigate the adjunctive diagnostic utility of hpv fusion proteins from the sera in the patients with cervical neoplasia. immunologic researches on host factors in hpv infection could contribute to better understanding of patho-physiologic mechanisms of cervical carcinogenesis, and provide valuable information for hpv vaccine development.19958640473
contraceptive use and reproductive history in women with cervical human papillomavirus infection.the study was conducted to investigate whether cervical human papillomavirus infections (chpi) are associated with contraceptive use and reproductive history. the contraceptive and reproductive histories in 972 women seeking contraceptive advice were noted and screening conducted for human papillomavirus infection. the interview included number of pregnancies and childbirths, legal and spontaneous abortions, and menstrual pattern. information about current use of contraceptive methods, about cas ...19958659312
[evaluation of sexual abuse in an infant with condyloma acuminatum].condyloma acuminatum is a skin infection affecting the anogenital area caused by the human papilloma virus and is usually sexually transmitted in adults. the disease in children is rare and the mode of transmission is controversial. because of the possibility that children with condyloma acuminatum have been sexually abused, a thorough medical and social evaluation is essential. however, the physician should bear in mind the possibility of nonsexual transmission. effective management of condylom ...19958682353
natural history of cervical human papillomavirus lesions.a total of 87 hpv-positive patients with grade i and ii cervical intraepithelial neoplasia (cin i and ii) were followed up by cytology and colposcopy every 3 months for more than 5 years following the first biopsy. these patients were classified into three groups (progressive, persistent, and regressive disease) according to the results. the human papillomavirus (hpv) genome and viral types were identified by southern blot hybridization at tm-40 degrees and tm-20 degrees with dna extracted from ...19958682616
[the treatment of neglected cases of condylomata acuminata in pregnant women with the nd:yag laser].the authors report for the therapeutical application of nd:yag laser with wave length 106 nm--medilas 4060n on a genital acute condyloma, caused by human papilloma virus at 19 pregnant women between 10-38 gestation week (g.w.). it is clinical studied a lot of exophytic formations on wide area included vulva, vagina, vaginal portion of uterine cervix (pvcu), perineum and anus. it is marked histological research of the biopsy material from the condyloma for excluding a malignant process, microbiol ...19958651421
a review of cervical cancer and cervical screening: implications for nursing practice.in this paper we review epidemiological and other research evidence on cervical cancer and cervical screening. we outline recent research evidence implicating sexually transmitted human papillomavirus as one of the causes of cervical cancer, but stress the uncertainty surrounding the causes and natural history of the disease. we go on to discuss evidence on risk factors associated with increased incidence of and mortality from cervical cancer, including age, sexual behaviour, smoking, socioecono ...19958708195
papillomavirus in cervicovaginal smears of women infected with human immunodeficiency virus.it has been described that women infected with the human immunodeficiency virus (hiv) present more frequent cytological abnormalities in cervicovaginal smears, generally related to infection by human papillomavirus (hpv). the present work is a study of cervicovaginal smears of 147 hiv-seropositive women submitted to routine gynecological examinations. the smears were stained by the papanicolaou method. cytopathic effects of hpv were found in 38 (25.8%) cases. nuclear atypias of cervical intraepi ...19958731285
[esophageal squamous cell papilloma. report of 3 cases. diagnostic aspects].dysphagia is not always present in patients with esophageal squamous cell papilloma, and it can be an incidental finding during gastrointestinal endoscopy of an asymptomatic patient.19958731606
lack of efficacy of interferon-alpha therapy in recurrent, advanced cervical cancer.human papillomavirus (hpv) is associated with 65-95% of in situ or early invasive squamous cell carcinomas of the cervix. a multiinstitutional, prospective phase ii clinical trial was initiated by the eastern cooperative oncology group (ecog) to study the activity of ifn-alpha 2b in women with metastatic or locally recurrent cervix cancer. the activity of ifn-alpha 2b was correlated with the presence of hpv as measured by southern blot hybridization or polymerase chain reaction techniques in 17 ...19958746781
presence of human papilloma virus in transitional cell carcinoma in jewish population in israel.the possible causal association of human papilloma virus (hpv) with transitional cell carcinoma (tcc) of the urinary bladder in israeli jewish patients was assessed. one hundred and ten histopathological tcc sections were examined by peroxidase anti-peroxidase (pap) method. hpv capsid antigen was demonstrated in 19 out of 110 cases (17.3%). hpv-dna sequences, determined by in situ dna-dna hybridization at high stringency wash were present in 24 cases (21.8%): 16(14.5%) cases proved to be hpv6/11 ...19958747082
detection of human papillomavirus dna in primary lung carcinoma by nested polymerase chain reaction.human papillomaviruses (hpv) have been implicated in the pathogenesis of human squamous cell carcinoma, especially of cervical carcinomas. in two previous studies concerning squamous cell carcinomas of the lung, dna of hpv subtypes 6/11/16/18 (and 31/33/35 for one study) was detected by in situ hybridization in 7% to 30% of the cases. a series of 31 frozen biopsies of lung carcinomas were examined for the presence of hpvdna by nested polymerase chain reaction (pcr). type-specific primers (6/11, ...19958747090
surrogate endpoint biomarkers for cervical cancer chemopreventive trials.cervical intraepithelial neoplasia (cin) represents a spectrum of epithelial changes that provide an excellent model for developing chemopreventive interventions for cervical cancer. possible drug effect surrogate endpoint biomarkers are dependent on the agent under investigation. published and preliminary clinical reports suggest retinoids and carotenoids are effective chemopreventive agents for cin. determination of plasma and tissue pharmacology of these agents and their metabolites could ser ...19958747385
bladder squamous cell carcinoma with human papilloma virus type 6 [hpv 6].a 91-year-old male was diagnosed with squamous cell carcinoma of the urinary bladder. molecular biological examination using the polymerase chain reaction (pcr) method demonstrated the presence of hpv type 6. hpv type 6 is considered very rare in urinary bladder malignancies.19958749958
transcriptional control of human papillomavirus type 18 oncogene expression in different cell lines: role of transcription factor yy1.binding of yy1 to the proximal fragment of the human papillomavirus type 18 (hpv-18) upstream regulatory region (urr) activates the oncogene expression of hpv-18 in hela cells, whereas in hepg2 cells this expression is repressed by yy1. in the present transient transfection study, we analyze the regulation of the hpv-18 urr by yy1 in an extended number of cell lines. except for hela cells, yy1 represses or does not influence oncogene expression in all cell lines tested. in hela cells the activat ...19958808335
squamous cell carcinoma of the tongue in a child with fanconi anemia: a case report and review of the literature.this report documents a case of squamous cell carcinoma (scc) of the tongue in a child with fanconi anemia (fa). fa is an autosomal recessive syndrome defined by chromosomal breakage in response to diepoxybutane or mitomycin c in which many patients present with pancytopenia, hypoplastic bone marrow, hyperpigmentation of the skin, skeletal malformations, small stature, hypogonadism, and chromosomal aberrations. such patients are prone to the development of hematological malignancies and squamous ...19958597846
detection of human papillomavirus type 57 in a case of inverted nasal papillomatosis in japan.human papillomavirus (hpv) type 57 dna was detected in recurrent nasal inverted papillomatosis, in a 60-year-old japanese male, using southern blot hybridization. hpv types 6, 11, 16 and 18 were not detectable in the papilloma. previous studies have shown that hpv types 6, 11, 16 and 18 were mainly found in nasal papillomatosis in other laboratories but the actual detection rate of virus was low. we speculate that this low detection rate may be due to the lack of a method for detecting hpv type ...19958719598
studies on in vivo induction of cytotoxic t lymphocyte responses by synthetic peptides from e6 and e7 oncoproteins of human papillomavirus type 16.induction of cytotoxic t lymphocyte (ctl) responses is an important defense mechanism against infectious agents, specifically viruses. in the present investigation we employed a mouse assay system we previously developed, for rapid induction of ctls by synthetic peptides from e6 and e7 oncoproteins of human papillomavirus type 16 (hpv-16). in particular, we compared the efficiency of ctl induction by hpv-16 peptides synthesized as linear monomers with those containing a dipalmitoyl-lysine-glycin ...19958833270
medical therapy of genital human papilloma virus-related disease. 19958845396
the atp-binding and atpase activities of human papillomavirus type 16 e1 are significantly weakened by the absence of prolines in its atp-binding domain.the e1 protein of human papillomavirus (hpv) type 16 is the only known papillomavirus e1 which does not contain any proline residues in the phosphate-loop (p-loop) of its atp-binding site. to ascertain whether this feature influences the activities of hpv-16 e1, we generated a mutant hpv-16 e1 (e1pro) in which prolines are inserted in place of alanines in this site, making the p-loop identical to its bovine papillomavirus type 1 counterpart. glutathione s-transferase (gst) fusion proteins (gste1 ...19958847499
sexually transmitted diseases in australia: a decade of change. epidemiology and surveillance.survival data in the last decade for sexually transmitted diseases (stds) other than human immunodeficiency virus (hiv) are uneven across the states/territories. the incidence of gonorrhoea decreased by more than 80%, but at different times in different patient groups, different states, and different anatomical sites. there was a resurgence of rectal gonorrhoea in homosexual men in 1989-1991. resistance to penicillin steadily increased, and partial resistance to quinolones has emerged. there was ...19958849191
genital oncogenic human papillomavirus infection: a short review on the mode of transmission.the most common oncogenic human papillomaviruses (hpvs) detected in the female lower genital tract are hpv types 16 and 18. they are ancient viruses which have evolved with mankind over the last 200 000 years. the identification of their roles in the development of carcinoma of the uterine cervix in the last decade fills the gap between the long-sought link between sexual behaviour and risk of cervical cancer. indeed, epidemiological, clinical and sophisticated molecular studies employing the po ...19958849195
detection of type specific human papillomavirus (hpv) dna in cervical cancers of indian women.commercial vira pap and vira type kits of life technologies inc., u.s.a., were used to determine prevalence and type specific distribution of human papilloma viruses (hpv) in 18 biopsy samples of cervical carcinomas and 26 specimens of exfoliated cervical cells (swabs). the women were either attending out-patient's department of a suburban hospital or a cancer hospital in calcutta, india. hpv dna was detected in 9 biopsy specimens but not in any of the cervical swabs. five of the 9 hpv positive ...19958919467
primary keratinocytes can be infected by natural isolates of genital human papillomavirus.viral particles of human papillomavirus (hpv) types 11 and 16 were isolated from flat condylomas and biopsies of dysplastic lesions and used to infect human keratinocytes. the presence of hpv dna in infected cells was determined by molecular hybridization and the polymerase chain reaction. late transcripts were detected shortly after infection. the persistence of hpv was limited to early passages after infection and the loss of viral dna preceded cell culture senescence. our experimental evidenc ...19958724856
detection of silencer activity in the long control regions of human papillomavirus type 6 isolated from both benign and malignant lesions.human papillomavirus type 6 (hpv-6) dna is the predominant hpv type found in condyloma acuminata: it is rarely found in carcinomas. we have previously reported cloning and characterizing an hpv-6 from a vulvar condyloma (hpv6-w50) and an hpv-6 from a vulvar carcinoma (hpv6-t70). the e5, e6 and e7 proteins encoded by the two genomes were identical, however, the two genomes differed in the long control region (lcr). cloning of the entire lcr into the enhancerless plasmid psvecat showed that the tw ...19959049328
transforming growth factor-beta 1 (tgf beta 1) enhances apoptosis in human papillomavirus type 16-immortalized human ectocervical epithelial cells.transforming growth factor beta (tgf beta) is a potent inhibitor of epithelial cell growth. in the present study tgf beta 1 modulation of human ectocervical epithelial cell growth and differentiation is evaluated using an hpv16-immortalized human ectocervical cell line, ece16-1. these cells were found to contain a high-affinity receptor for tgf beta 1 (kd = 75 pm). tgf beta (10-500 pg/ml) suppressed ece16-1 growth and [3h]thymidine incorporation in a dose-dependent manner. growth inhibition was ...19957529191
association of serum immunoglobulin g antibodies against human papillomavirus type 16 capsids with anal epidermoid carcinoma.anal epidermoid carcinoma is a relatively rare tumor, but its incidence has been increasing rapidly during the past few years. genetic material from the major oncogenic types of human papillomavirus (hpv), types 16 and 18, has regularly been demonstrated in a substantial proportion of anal cancers, suggesting an etiologic role of hpv infection. recently, serum antibodies against hpv type 16 capsids were shown to be a serologic measure of hpv16 infection.19957532227
differential regulation of human papillomavirus type 6 and 11 early promoters in cultured cells derived from laryngeal papillomas.cells cultured from laryngeal papillomas contain episomal human papillomavirus type 6 or type 11 (hpv-6/11) dna. we developed a sensitive rnase protection assay to simultaneously measure expression from the hpv e6, e7, and e1 promoters (p1, p2 and p3, respectively) in this manipulable culture system and found that p1, p2 and p3 transcript abundances could be independently modulated by culture medium composition and culture substrate. in undifferentiated cells grown in a low-calcium, serum-free m ...19957474101
the viral oncoproteins ad5 e1a, hpv16 e7 and sv40 tag bind a common region of the tbp-associated factor-110.a function shared by the adenovirus e1a, papillomavirus e7 and sv40 tag oncoproteins is their ability to interfere with normal cell growth by interacting with members of the retinoblastoma protein family. in this study, we show that each of these oncoproteins can also bind to the 921 amino acid tbp-associated factor-110 (taf-110). the significance of the binding is underscored by the observation that each oncoprotein binds to the same 77 amino acid carboxyl region of taf-110. in the case of e1a ...19957478615
diminished capacity for p53 in mediating a radiation-induced g1 arrest in established human tumor cell lines.it has been reported that the p53 gene mediates an ionizing radiation-induced g1 arrest in mammalian cells. to further characterize this important phenomenon, a panel of seven human diploid fibroblast cell strains and 14 human tumor cell lines from a variety of sources with both wild-type and mutant p53 status were assayed for their susceptibility to g1 arrest after gamma-ray irradiation by a continuous labeling [3h]thymidine incorporation technique. an irreversible g1-block involving 20-70% of ...19957478618
spectrum of genital human papillomavirus infection in a female adolescent population.human papillomavirus infection is a sexually transmitted disease associated with cervical dysplasia and carcinoma.19957482107
analysis of p53 tumor suppressor gene mutations and human papillomavirus infection in human bladder cancers.to determine whether the dysfunction of p53 caused either by mutation of the p53 gene itself or by binding to e6 protein of oncogenic hpvs is involved in the transitional cell carcinomas (tccs) of the bladder, we analyzed 23 tccs of the bladder. dna was extracted from each paraffin embedded tissue of tccs of bladder and polymerase chain reaction (pcr)/single strand conformation polymorphism (sscp) analysis were performed to screen mutations in p53 tumor suppressor gene, then pcr/dot blot hybridi ...19957483675
alterations in physical state and expression of human papillomavirus type 18 dna following crisis and establishment of immortalized ectocervical cells.integration of episomal human papillomavirus (hpv) dna in infected cervical lesions during malignant progression is frequently observed, but the importance of integration is poorly understood. we have studied immortalization by hpv-18 of human cervical cells as an in vitro model system. here, the status and expression of hpv-18 dna in precrisis ectocervical keratinocytes was compared with that in the same cells after crisis and establishment of immortalization. southern blots revealed, and two-d ...19957483827
[dna papillomavirus detection in head and neck benign proliferative lesions].in a retrospective study, human papillomavirus (hpv) (6/11, 16 and 18 types) were tested using polymerase chain reaction (pcr), in stored formalin-fixed, paraffin-embedded tissue sections, from 36 head and neck adult papillomata. the results, only 20 percent of positive cases, are non consistent with the role of the hpv infection in the etiology of head and neck papillomata in adult patients. however we detect hpv-18 positivity in papillomata with dysplasia.19957485857
detection of human papillomavirus dna in nongenital seborrhoeic keratoses.the histological similarities of seborrhoeic keratoses and common warts led to the investigation of the possible occurrence of human papillomavirus dna (hpv-dna) in a large number of nongenital seborrhoeic keratoses using the in situ hybridization technique. all specimens derived from normal skin (n = 173) were negative for the applied hpv-dna probe, whereas the hpv genome was detected in 34 of 173 seborrhoeic keratosis specimens (19.65%). of 34 hpv-positive specimens, 15 contained types 6/11 an ...19957487151
excessive numbers of skin cancers and pre-malignant skin lesions in an australian heart transplant recipient.one and a half years after heart transplantation an australian man developed his first skin cancer. in the period until his death, 7 years after the transplantation, 34 histologically confirmed squamous cell carcinomas developed, 17 intra-epidermal carcinomas, and 9 basal cell carcinomas. most skin cancers were confined to chronically sun exposed sites. exposure to sunlight and human papillomavirus are important factors in the development of skin cancer in renal transplant recipients, and furthe ...19957488467
marker genes for cytotoxic exposure: p53.the growth suppressor p53 plays an important role in the regulation of cell proliferation, dna repair and apoptosis. in wild-type p53 expressing cells, gamma-irradiation induces an increase in the level of p53 protein and these cells exhibit a g1 growth arrest. the p53-induced g1 growth arrest is abrogated in cells expressing mutant p53, or in cells where p53 is inactivated by complex formation with cellular or viral proteins such as mdm2 or the e6 proteins of human papillomavirus (hpv) 16 or hp ...19957488939
building up a computerized follow-up register and information system for cervical cytology.it is well known that cervical intraepithelial neoplasia (cin) and human papilloma virus (hpv) infection are closely related to cervical cancer. the cervical cytology examination (pap-test), followed by a colposcopic examination and a biopsy are the common tools for the early detection and the establishment of diagnosis of the cervical precancerous lesions. the monitoring and surveillance of those precancerous lesions is essential for the prevention of cervical cancer and for effective patient m ...19957490162
management of low-grade cin: follow-up or treatment?to examine whether low-grade cervical dysplasia carries a higher risk of progression when associated with the cancer-related human papillomavirus types 16, 18, 31 or 33.19957493708
oncogenicity of human papillomavirus- or adenovirus-transformed cells correlates with resistance to lysis by natural killer cells.the reasons for the dissimilar oncogenicities of human adenoviruses and human papillomaviruses (hpv) in humans are unknown but may relate to differences in the capacities of the e1a and e7 proteins to target cells for rejection by the host natural killer (nk) cell response. as one test of this hypothesis, we compared the abilities of e1a- and e7-expressing human fibroblastic or keratinocyte-derived human cells to be selectively killed by either unstimulated or interferon (ifn)-activated nk cells ...19957494272
preneoplastic transformation of human mammary epithelial cells.recent in-vitro models of mammary epithelial cell (mec) immortalization have provided a practical approach to begin to dissect the molecular mechanisms of breast tumorigenesis. introduction of a single oncogene, the human papilloma virus (hpv)-16 e6, induces efficient and reproducible preneoplastic transformation of normal mecs, by inducing degradation of the tumor suppressor protein p53. the role of p53 has also been demonstrated by analyses of a model of gamma-radiation-induced mec transformat ...19957495987
high incidence of human papillomavirus in 146 cervical carcinomas. a study using three different pairs of consensus primers, and detecting viral genomes with putative deletions.polymerase chain reaction (pcr) primer sets and probe-cocktails were used for human papillomavirus (hpv) detection and typing of 146 fresh frozen biopsies of cervical carcinoma. we obtained a high detection rate (96%) by using three sets of consensus primer pairs directed at the l1 and e1 regions of hpv and by probing with a cocktail of random-labelled consensus and type-specific pcr products derived from hpv plasmids. in addition, we performed type-specific pcr amplification with e6-e7 primers. ...19957577081
p53 mutations in human papillomavirus-associated oesophageal squamous cell carcinoma. 19957577493
a transcribed human sequence related to the mouse hc1 and the human papillomavirus type 18 e5 genes is located at chromosome 7p13-14.the papillomavirus e5 genes play an important role in the induction of proliferation of infected cells, and these hpv genomic regions are affected by the events leading to integration of genital hpvs. two hpv18 e5-related, transcribed mouse sequences, hc1 and q300, have recently been described. we searched for human equivalents to these sequences, and isolated a clone with a 9.6 kb insert (633b) from a laryngeal carcinoma dna library, that strongly cross-hybridised with both the hpv18 e5 and hc1 ...19957581372
detection of human papillomavirus types 16, 18 dna related sequences in bronchogenic carcinoma by polymerase chain reaction.in studying the relationship between human papillomavirus (hpv) and bronchogenic carcinoma, "high-risk" hpv 16, 18 dna sequences were detected in samples from 50 lung cancer patients, 18 patients with benign pulmonary diseases and 4 fetal lung tissues by polymerase chain reaction (pcr) and dot-blot hybridization with biotin-labelled probes. the results showed that hpv 16, 18 dna related sequences were found in 32% of lung cancer specimens, with 10 cases of hpv 16, 5 cases of hpv 18 and 1 case of ...19957587494
cdkn2 in hpv-positive and hpv-negative cervical-carcinoma cell lines.human cervical cancers frequently contain retinoblastoma protein (rb) that is inactivated by binding with human papilloma virus (hpv) e7 protein or through mutation. the cdkn2 gene encodes p16ink4 which inhibits cdk4-cyclin d phosphorylation of rb, preventing the g1-s transition. to determine whether abnormalities of cdkn2 occur in cervical-cancer cells, ii cervical cell lines, including 8 hpv-positive cell lines, 2 hpv-negative cell lines containing mutant rb, and one tumorigenic cell line deri ...19957591209
are human papillomavirus infections not necessary or sufficient causal factors for invasive cancer of the cervix? 19957591223
anguinomycins c and d, new antitumor antibiotics with selective cytotoxicity against transformed cells.the retinoblastoma protein (prb) is inactivated during the development of a wide variety of human cancers. in the course of our screening for antitumor antibiotics by using prb-inactivated cells, an actinomycete strain was found to produce two active substances, which were elucidated to be new members of the leptomycin-anguinomycin family by nmr spectral analysis and were designated anguinomycins c and d. the anguinomycins induced growth arrest against normal cells and induced cell death against ...19957592062
deletion in human chromosome region 12q13-15 by integration of human papillomavirus dna in a cervical carcinoma cell line.in human cervical carcinomas papillomavirus dna is frequently integrated in the cell genome. we have cloned the integration site of human papillomavirus-18 dna in human chromosome region 12q13-15 present in the sw756 cervical carcinoma cell line. viral dna is broken from nucleotides 2643 to 3418 in the e1 and e2 open reading frames, resulting in a deletion of 775 bases of viral dna. cloning and sequence analysis of the rearranged and germline alleles shows that there is no homology between the t ...19957592643
evaluation of seroreactivity to human papillomavirus type 16 virus-like particles in an incident case-control study of cervical neoplasia.an elisa to detect serum igg antibody response to human papillomavirus (hpv) type 16 virus-like particles (vlps) was evaluated in a case-control study of cervical neoplasia, nested within a prospective cohort study. subjects included 688 controls with continued normal cytology and 152 cases with confirmed incident squamous intraepithelial lesions who were tested for dna for a broad spectrum of hpv types at cohort and follow-up of controls, 16.6% were seropositive compared with 30.8% and 52.4% of ...19957594698
divergent human papillomavirus type 16 variants are serologically cross-reactive.it is not known whether dna sequence variants of human papillomavirus type 16 (hpv-16) are distinct serotypes. to examine this question, the reactivities of women's sera from zaire (n = 97) and denmark (n = 123) were compared in igg-specific elisas based on virus-like particles (vlps) composed of the l1 major capsid protein derived from an hpv-16 variant common in central africa (z-1194) or one common in northern europe (114k). these l1s differ in seven amino acids. there was a strong correlatio ...19957594721
cervical intraepithelial neoplasia is not associated with elevated serum neopterin levels.neopterin, a marker of cellular immune activation, was elevated in patients who had cervical cancer in previous studies. to examine neopterin in the presence of precursors to cervical cancer (i.e., cervical intraepithelial neoplasia) we measured serum levels in 185 colposcopy patients in jamaica, a country with high cervical cancer incidence, and in 72 age-matched jamaican women selected from a large population-based sample. we also measured serum levels of beta-2-microglobulin, another commonly ...19957606206
expression of human papillomavirus type 16 e6-e7 open reading frame varies quantitatively in biopsy tissue from different grades of cervical intraepithelial neoplasia.the proteins encoded by the human papillomavirus type 16 e6-e7 open reading frame are essential for transformation of the host cell. two mrna species, e6*i and e6*ii, generated by alternative splicing of a polycistronic pre-mrna, encode truncated e6 proteins and the e7 protein. our investigation assessed whether or not the level of expression of e6*i and e6*ii varies quantitatively in relation to the grade of cervical intraepithelial neoplasia (cin). we used a quantitative reverse transcription ...19957615724
detection of antibodies against human papillomavirus (hpv) type 16 virions by enzyme-linked immunosorbent assay using recombinant hpv 16 l1 capsids produced by recombinant baculovirus.the l1 major capsid protein of human papillomavirus type 16 (hpv-16) was expressed in sf-21 insect cells with a recombinant baculovirus. virus-like particles obtained were purified and used to develop an enzyme-linked immunosorbent assay for detection of anti-hpv-16 antibodies in sera from 76 women with evidence of genital hpv infection and 79 controls. hpv-16-infected individuals developed antibodies directed at hpv-16 virions since reactivity against recombinant hpv-16l1 capsids was observed i ...19957615761
p53 in human papillomavirus associated anogenital cancers. 19957615867
skin-associated lymphoid tissue in human immunodeficiency virus-1, human papillomavirus, and herpes simplex virus infections.the skin-associated lymphoid tissue is composed of keratinocytes, langerhans cells, skin trophic t cells, and lymphatic endothelial cells of the skin. the epidermis, which is involved in many viral infections, contains all of the components needed for an effective immune response: antigen-presenting langerhans cells, t cells, and cytokines from leukocytes and keratinocytes. there have been some recent advances in the study of the cutaneous immunology involved in infections with the human immunod ...19957616007
transcriptional repression of smooth-muscle alpha-actin gene associated with human papillomavirus type 16 e7 expression.to explore the role of the e7 viral oncogene from human papillomavirus type 16 (hpv 16) in the regulation of cytoskeletal organization, we investigated alterations in particular cytoskeletal components in rat embryonal fibroblasts and three transformants of rat embryonal fibroblast cells produced by transfections with hpv16 e7 alone (tf1), hpv16 e7 plus adenovirus type 5 e1b (tf3), and hpv16 e7 plus activated ha-ras (tf4). marked reductions in smooth-muscle (sm) alpha-actin content and disrupted ...19957619218
t-helper epitopes of the e7 transforming protein of cervical cancer associated human papillomavirus type 18 (hpv18).the presence of t-helper epitopes within the e7 transforming protein of human papillomavirus type 18 (hpv18) was sought using a series of overlapping synthetic 15-20 mer peptides spanning the entire 105 amino acid sequence of this protein. two h-2k restricted t-helper epitopes were defined, comprising 44vnhqhlparra55 and 81ddlrafqqlf90 as the minimal t proliferative epitopes. peptides containing these epitopes were able to provide cognate help to b epitopes from hpv18e7 protein for production of ...19957542826
detection of antibodies to l1, l2, and e4 proteins of human papillomavirus types 6, 11, and 16 by elisa using synthetic peptides.antibodies against eight synthetic peptides spanning different epitopes located on l1, l2, and e4 proteins of human papillomavirus (hpv) types 16, 6, and 11 were examined in sera from 73 women infected by hpv and from 139 healthy controls. only three of these peptides were reactive. two located on proteins l2 and e4 of hpv 16 seem type specific since antibodies to these peptides were detected, respectively, in 21% and 15% of the hpv 16 infected patients and in 2.5% and none of women infected by ...19957545211
the chronically symptomatic vulva: aetiology and management.to determine the causes and management of chronic vulval symptoms and to compare the findings in patients first presenting to a gynaecologist with those in patients first presenting to a dermatologist.19957547732
search for evidence of three viral agents in radicular (periapical) cysts with immunohistochemistry.sections from 20 paraffin-embedded radicular cysts and positive-control subjects were subjected to immunostaining procedures after treatment of the sections with polyclonal antibodies was performed to search for evidence of human papilloma virus and herpes viruses 1 and 2 in the lining epithelium. all 20 specimens examined were negative for the presence of all three viruses.19957552869
[cytologic-colposcopic-histopathologic correlations in preinvasive cervical lesions and cervical human papillomavirus infections].cervico-uterine cancer is the most frequent gynecological neoplasia in mexico and cervico-vaginal cytology is the most practical and dependable resource in lesions detection. during the last years precursory lesions detection (nic and hpv infection) has increased. so, every patient presenting with an abnormal cytology should be included in an evaluation program, that includes a colposcopic study with biopsy of suspicious lesions, in order to know cellular abnormality degree, as these studies com ...19957557531
cervical dysplasia in hiv-seropositive women: role of human papillomavirus infection and immune status.in the present study we investigated the cytologic and colposcopic characteristics of a cohort of hiv-infected women, with the aim to determine a relationship between immunologic status and frequency and/or severity of cervical abnormalities.19957557645
detection of human papillomavirus dna in cervical lavage specimens by a nonisotopic consensus pcr assay.a gene amplification method that combines pcr with an enzyme immunoassay (pcr-eia) for quantitation of amplified dna was developed for the detection of human papillomavirus (hpv). samples were amplified with consensus primers my09 and my11. amplified dna products were reacted in solution with type-specific nested rna probes labelled with digoxigenin-11-utp. hybrids were captured on a microtiter plate coated with an antidigoxigenin antibody. bound dna-rna hybrids were quantitated by the addition ...19957559932
human papillomavirus (hpv) type distribution and serological response to hpv type 6 virus-like particles in patients with genital warts.thirty-nine patients with condylomas (12 women and 27 men) attending a dermatology clinic were tested for genital human papillomavirus (hpv) dna and for seroprevalence to hpv type 6 (hpv6) l1 virus-like particles. the l1 consensus pcr system (with primers my09 and my11) was used to determine the presence and types of hpv in sample specimens. all 37 (100%) patients with sufficient dna specimens were positive for hpv dna, and 35 (94%) had hpv6 dna detected at the wart site. three patients (8%) had ...19957559948
physical state of human papillomavirus using non-isotopic in situ hybridisation. 19957560216
sequelae of premature sexual life.premature sexual activity among adolescents leads to such complications as unwanted pregnancy, abortion, and sexually transmitted diseases (stds). the increasing adolescent sexual intercourse worldwide will likely result in increasing rates of adolescent pregnancy, although a recent study has shown that these rates will vary from country to country. rates of pregnancy for girls younger than 14 are very low worldwide, and the cesarean rate is also lower than that found among older women. the m ...19957562803
xeroderma pigmentosum variant cells are resistant to immortalization.xeroderma pigmentosum (xp) is a human repair-deficient disorder that is caused by mutations in any of eight genes (a-g, v). the genes for complementation groups a-g have been cloned fully or in part, but the gene for the xp variant (xpv) has yet to be cloned. the lack of progress with xpv is in large part due to the rarity of stably transformed cell lines. we have attempted to immortalize fibroblasts from several xpv patients to obtain cell lines with which to characterize this disease and clone ...19957565859
postmenopausal squamous atypia: a spectrum including "pseudo-koilocytosis".in addition to typical atrophy, the postmenopausal cervix may exhibit a spectrum of epithelial and cellular alterations, including prominent perinuclear halos, nuclear hyperchromasia, variation in nuclear size, and multinucleation. it has not been determined whether such changes, termed postmenopausal squamous atypia (psa), represent age-related epithelial disturbances or human papillomavirus (hpv)-related low-grade squamous intraepithelial lesions (condyloma). we surveyed 30 cervical biopsies f ...19957567940
human papillomavirus type 11 transcripts are present at low abundance in latently infected respiratory tissues.respiratory tract tissues containing latent human papillomavirus (hpv) 11 were analyzed by reverse transcription-polymerase chain reaction for the presence of viral-specific rna from the early region of the genome and compared to a similar analysis of laryngeal papillomas. latently infected tissue contained low-abundance transcripts that could code for e1 and e2 proteins, but lacked evidence of spliced transcripts for the e6 and e7 proteins. both latently infected tissue and papilloma tissue con ...19957571398
high grade anal dysplasia in visually normal mucosa in homosexual men: seven cases.anal cancer and anal human papillomavirus (hpv) infection are increased in homosexual men.19957572893
a study comparing letz and co2 laser treatment for cervical intra epithelial neoplasia with and without associated human papilloma virus.to evaluate the recurrence of cervical intraepithelial neoplasia (cin) following treatment with co2 laser ablation and loop excision of the transformation zone (letz) and its correlation with the presence of hpv.19957641744
the hpv16 e5 protein: expression, detection, and stable complex formation with transmembrane proteins in cos cells.the human papillomavirus-16 (hpv16) e5 gene is able to induce stable growth transformation and transient mitogenic stimulation in a variety of cultured cell systems. to characterize the biochemical properties of the hydrophobic hpv16 e5 transforming protein, we have constructed vectors expressing the wild-type hpv16 e5 gene and have generated antipeptide antisera. the 10-kda e5 protein was readily detectable in transfected cos monkey cells by using these antisera either for immunoprecipitation o ...19957645215
human papillomavirus vulvitis: a new disease or an unfortunate mistake? 19957647068
regulation of the cell cycle by viral oncoproteins.human papillomavirus (hpvs) adenovirus and simian virus 40 (sv40) are small dna viruses which can show oncogenic activity. although not otherwise related, all three have adopted very similar strategies to deregulate cell growth; each virus encoding oncoproteins which interact with the same cellular targets. of particular interest are the interactions with the cell encoded prb and p53 proteins, products of tumour suppressor genes. somatic mutation results in the loss of the prb and p53 function i ...19957647307
a recurrent human papillomavirus integration site at chromosome region 12q14-q15 in sw756 and sk-v cell lines derived from genital tumors.the sw756 cell line, derived from an invasive cancer of the uterine cervix, harbours integrated human papillomavirus (hpv) 18 dna sequences which have been located in chromosome band 12q13. by in situ hybridization experiments with tritiated and digoxigenin-labelled hpv18 probes on r-banded chromosomes, we now localize the integrated viral sequences in 12q14-q15. interestingly, we have previously localized integrated hpv16 sequences in the same chromosomal region in sk-v cells, derived from a pr ...19957648359
genital human papillomavirus infection: common but not trivial.one of the most prevalent sexually transmitted diseases is genital human papillomavirus (hpv) infection. because of its potentially serious consequences for women, hpv infection is receiving a significant amount of attention from researchers and clinicians. we summarize the research and clinical literature on hpv infection. the nature of the virus, the prevalence of hpv infection, and modes of transmission are discussed. both the physiological and psychological consequences of the infection are ...19957649892
human papillomavirus testing in primary cervical screening. 19957651020
conditional immortalization of primary cells by human papillomavirus type 18 e6 and ej-ras defines an e6 activity in g0/g1 phase which can be substituted for mutations in p53.the human papillomavirus (hpv) type 18 e6 gene cooperates with activated ha-ras to immortalize primary mouse cells in culture. using a plasmid where hpv18 e6 expression is regulated by the glucocorticoid inducible mmtv ltr, we have generated immortalized cell lines in which the continued expression of e6 was necessary for maintenance of the transformed phenotype. in the absence of exogenously added hormone these cells were found to arrest in g0/g1. furthermore, we demonstrate that the effects of ...19957651728
seric and local antibodies against a synthetic peptide of hpv16.an elisa method was used to detect igg and iga directed against a synthetic peptide derived from the e2 orf of the human papillomavirus (hpv) 16 in sera and in cervico-vaginal secretions from 20 women without evidence of hpv infection and from 41 women with histological diagnosis of hpv infection. the proportion of iga positive sera (63.4% in the case-group vs. 20.0% in the control-group) and secretions (48.8% in the case-group vs. 15.0% in the control-group) was significantly higher in women wi ...19957657014
transmission of cervical human papillomavirus infection by sexual activity: differences between low and high oncogenic risk types.not all studies have proven that cervical human papillomavirus (hpv) infection is a sexually acquired condition. determinants of hpv infection were analyzed in a survey of 718 asymptomatic women in northeastern brazil. hpv dna was detected and typed by polymerase chain reaction. hpv types were classified into low- and high-risk groups on the basis of their association with cervical carcinomas. overall hpv prevalence (18.3%) was moderately associated with age at first intercourse (p = .111, trend ...19957658069
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