Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
listeria monocytogenes pleuritis: report of a geriatric case. 201323551348
cd30l/cd30 is critical for maintenance of il-17a-producing γδ t cells bearing vγ6 in mucosa-associated tissues in mice.cd30 ligand (cd30l, cd153), a member of the tumor necrosis factor (tnf) superfamily, and its receptor cd30 are important for differentiation and activation of cd4(+) t helper type 17 (th17) cells. in this report, we demonstrate that the interleukin 17a (il-17a)-producing γδ t cells normally developed in the fetal thymus, whereas vγ1(-)vγ4(-) γδ t cells expressed vγ6/vδ1 gene transcript selectively decreased in mucosa-associated tissues in naive cd30ko or cd30lko mice. moreover, cd30 and cd30l we ...201323549449
modeling the growth of listeria monocytogenes in mold-ripened cheeses.this study presents possible applications of predictive microbiology to model the safety of mold-ripened cheeses with respect to bacteria of the species listeria monocytogenes during (1) the ripening of camembert cheese, (2) cold storage of camembert cheese at temperatures ranging from 3 to 15°c, and (3) cold storage of blue cheese at temperatures ranging from 3 to 15°c. the primary models used in this study, such as the baranyi model and modified gompertz function, were fitted to growth curves. ...201323548297
physical-mechanical and antimicrobial properties of nanocomposite films with pediocin and zno nanoparticles.this work aimed to develop nanocomposite films of methyl cellulose (mc) incorporated with pediocin and zinc oxide nanoparticles (nanozno) using the central composite design and response surface methodology. this study evaluated film physical-mechanical properties, including crystallography by x-ray diffraction, mechanical resistance, swelling and color properties, microscopy characterization, thermal stability, as well as antimicrobial activity against staphylococcus aureus and listeria monocyto ...201323544529
magnetic nano-beads based separation combined with propidium monoazide treatment and multiplex pcr assay for simultaneous detection of viable salmonella typhimurium, escherichia coli o157:h7 and listeria monocytogenes in food products.we developed a rapid and reliable technique for simultaneous detection of salmonella typhimurium, escherichia coli o157:h7 and listeria monocytogenes that can be used in food products. magnetic nano-beads (mnbs) based immunomagnetic separation (ims) was used to separate the target bacterial cells while multiplex pcr (mpcr) was used to amplify the target genes. to detect only the viable bacteria, propidium monoazide (pma) was applied to selectively suppress the dna detection from dead cells. the ...201323541211
antimicrobial resistance and co-selection phenomenon in listeria spp. recovered from food and food production environments.antimicrobial resistance (amr), co-selection phenomenon, and the relationship between reduced susceptibility (rsc) to ciprofloxacin (cip) and resistance to other antimicrobials in listeria spp. (n = 103) recovered from food processing environments (fpe) and food were investigated. resistance of listeria monocytogenes and other listeriae, respectively, to cefoxitin (fox; 98% vs. 88%), cip (7% vs. 4%), clindamycin (cli; 33% vs. 59%) and tetracycline (6% vs. 8%) was observed, as was rsc to cip (67% ...201323541198
the application of high-concentration short-time chlorine dioxide treatment for selected specialty crops including roma tomatoes (lycopersicon esculentum), cantaloupes (cucumis melo ssp. melo var. cantaloupensis) and strawberries (fragaria×ananassa).the effects of high-concentration short-time chlorine dioxide (clo2) gas treatment on food-borne pathogens inoculated onto the surface of tomatoes, cantaloupes, and strawberries were studied. produce were spot-inoculated with a mixture of salmonella enterica (serotypes montevideo, javiana and baildon), escherichia coli o157:h7 (serotypes 204 p, edl 933 and c792) or listeria monocytogenes (serotypes scott a, f 5069 and lcdc 81-861), and treated with clo2 gas at 10 mg/l for 180 s. after clo2 gas t ...201323541196
combination use of pfge and drug-resistant analysis in the epidemiological investigation of listeria monocytogenes. 201323534473
[cerebral listeria abscess in a patient with gastric cancer].history and presentation on admission: a 70-year-old woman with a history of gastric cancer and palliative chemotherapy was admitted with disturbed consciousness.201323533043
comparative analysis of the exoproteomes of listeria monocytogenes strains grown at low temperatures.this article presents the major differences in the exoproteomes of listeria monocytogenes strains grown at 11°c and 20°c, and their comparison to 37°c, the optimal temperature of growth of this foodborne pathogenic bacteria. a set of four strains previously characterized and representing the genetic diversity of the species was used. two were virulent, of which one was persistent, and two were low virulent strains. the proteins secreted by the strains grown in minimal medium were separated by so ...201323531123
listeria monocytogenes arca contributes to acid tolerance.the foodborne pathogen listeria monocytogenes is able to colonize the human and animal intestinal tracts and subsequently crosses the intestinal barrier, causing systemic infection. for successful establishment of infection, l. monocytogenes must survive and adapt to the low ph environment of the stomach. gene sequence analysis indicates that lmo0043, an orthologue of arca, encodes a protein containing conserved motifs and critical active amino acids characteristic of arginine deiminase that med ...201323518652
cross-recognition of aspergillus galactomannan caused by listeria monocytogenes infection.we report a case of listeria monocytogenes bacteremia in a leukemic patient having a positive assay for aspergillus galactomannan (gm), although no evidence of aspergillosis was found. supernatant obtained from l. monocytogenes strain suspension was reactive with gm-assay. l. monocytogenes produces a soluble antigen that is cross-reactive with aspergillus gm.201323518185
fas-mediated inflammatory response in listeria monocytogenes infection.the molecular mechanisms of fas (cd95/apo-1)-mediated apoptosis are increasingly understood. however, the role of fas-mediated production of proinflammatory cytokines such as il-18 and il-1β in bacterial infection is unclear. we demonstrate the importance of fas-mediated signaling in il-18/il-1β production postinfection with listeria monocytogenes without the contribution of caspase-1 inflammasome. il-18/il-1β production in l. monocytogenes-infected peritoneal exudate cells from fas-deficient mi ...201323509366
structural and dynamic features of cold-shock proteins of listeria monocytogenes, a psychrophilic bacterium.cold-shock proteins (csps), proteins expressed when the ambient temperature drops below the growth-supporting temperature, bind to single-stranded nucleic acids and act as rna chaperones to regulate translation. listeria monocytogenes is a psychrophilic food-borne pathogen that is problematic for the food industry. structures of csps from psychrophilic bacteria have not yet been studied. despite dramatic differences in the thermostability of csps of various thermophilic microorganisms, these pro ...201323506337
cellular-flip, raji isoform (c-flip r) modulates cell death induction upon t-cell activation and infection.dysregulation of apoptosis caused by an imbalance of pro- and anti-apoptotic protein expression can lead to cancer, neurodegenerative, and autoimmune diseases. cellular-flip (c-flip) proteins inhibit apoptosis directly at the death-inducing signaling complex of death receptors, such as cd95, and have been linked to apoptosis regulation during immune responses. while the isoforms c-flipl and c-flips are well characterized, the function of c-flipr remains poorly understood. here, we demonstrate th ...201323505065
gene expression profiling of a nisin-sensitive listeria monocytogenes scott a ctsr deletion mutant.listeria monocytogenes is a food-borne pathogen of significant threat to public health. nisin is the only bacteriocin that can be used as a food preservative. due to its antimicrobial activity, it can be used to control l. monocytogenes in food; however, the antimicrobial mechanism of nisin activity against l. monocytogenes is not fully understood. the ctsr (class iii stress gene repressor) protein negatively regulates the expression of class iii heat shock genes. a spontaneous pressure-tolerant ...201323494707
susceptibility of aged guinea pigs to repeated daily challenge with listeria monocytogenes.epidemiological data suggests that certain groups such as the elderly are more susceptible to listeriosis than the general population. repeated exposure to low levels of listeria monocytogenes may increase the probability of infection. increased susceptibility to infection in the elderly has been attributed in part to reduced activity of t cells. we investigated the impact of consecutive daily intragastric challenge with l. monocytogenes on the development of listeriosis within an aged guinea pi ...201323489051
rapid and sensitive immunodetection of listeria monocytogenes in milk using a novel piezoelectric cantilever sensor.listeria monocytogenes (lm), an important food-borne pathogen that has a high mortality rate (≈ 30%), was successfully detected within an hour at the infection dose limit of 10(3)/ml, both in buffer and milk. lm detection was demonstrated using a novel asymmetrically anchored cantilever sensor and a commercially available antibody. sensor responses were confirmed using a secondary antibody binding step, similar to the sandwich elisa assays, as a means of signal amplification that also reduced th ...201323475140
preservative action of 11s (glycinin) and 7s (β-conglycinin) soy globulin on bovine raw milk stored either at 4 or 25 °c.considerable inhibitory antibacterial actions were exerted by the soybean 11s subunit comparable with nisin on the proliferation of total viable count, pseudomonas count and enterobacteriaceae count in bovine milk stored at 4 or 25 °c for 30 d and 48 h, while 7s and lysozyme were much less effective. the maximum magnitudes of bacterial reduction by 11s and nisin were in the range 2-4 log cfu/ml. the proliferation of 3 pathogenic bacteria (listeria monocytogenes, salmonella enteritidis and escher ...201323473420
successful vaccination of immune suppressed recipients using listeria vector hiv-1 vaccines in helminth infected mice.vaccines for hiv, malaria and tb remain high priorities, especially for sub-saharan populations. the question is: will vaccines currently in development for these diseases function in populations that have a high prevalence of helminth infection? infection with helminth parasites causes immune suppression and a cd4+ th2 skewing of the immune system, thereby impairing th1-type vaccine efficacy. in this study, we conduct hiv vaccine trials in mice with and without chronic helminth infection to mim ...201323470236
antimicrobial efficacy of poly (dl-lactide-co-glycolide) (plga) nanoparticles with entrapped cinnamon bark extract against listeria monocytogenes and salmonella typhimurium.nanoencapsulation of active compounds using poly-(d,l-lactide-co-glycolide) (plga) is commonly used in the pharmaceutical industry for drug delivery and may have important applications in the food industry. control of growth of foodborne bacteria with the goals of reducing the number of foodborne illness outbreaks, assuring consumers a safer food supply remains a priority in the food industry. natural antimicrobials are an excellent way to eliminate pathogens without introducing chemical preserv ...201323464835
antimicrobial activity of nanodispersed thymol in tryptic soy broth.food safety is a continuing challenge for the food industry due to sporadic illness outbreaks caused by foodborne pathogens. plant essential oils have been studied extensively as natural antimicrobials to control foodborne pathogens. however, their hydrophobic nature makes application in foods difficult because of their low water solubility, adverse impact on sensory quality, and binding with food components, which can interfere with antimicrobial efficacy. the objective of this study was to cha ...201323462081
frequency of salmonella and listeria monocytogenes in five commercial brands of chicken eggs using a combined method of enrichment and nested-pcr.eggs or egg-based foods, either raw or undercooked, have been identified as vehicles of salmonella outbreaks. the low numbers of salmonella organisms in eggs makes it difficult to detect them in frequency studies. the nested-pcr (n-pcr) technique shows more sensitivity and specificity than bacteriological culture methods (bcms). a preenrichment method followed by enrichment and n-pcr is a good alternative for the investigation of salmonella and listeria monocytogenes in eggs. a total of 2,650 ch ...201323462079
commercial thermal process for inactivating salmonella poona on surfaces of whole fresh cantaloupes.outbreaks of salmonellosis by salmonella poona and listeriosis by listeria monocytogenes have been associated with the consumption of cantaloupes. commercial washing processes for cantaloupes are limited in their ability to inactivate and/or remove this human pathogen. our objective was to develop a commercial-scale surface pasteurization process for enhancing microbiological safety of cantaloupes. whole cantaloupes, surface inoculated with salmonella poona rm 2350, were stored at 32°c for 24 h ...201323462078
fda-irisk--a comparative risk assessment system for evaluating and ranking food-hazard pairs: case studies on microbial hazards.stakeholders in the system of food safety, in particular federal agencies, need evidence-based, transparent, and rigorous approaches to estimate and compare the risk of foodborne illness from microbial and chemical hazards and the public health impact of interventions. fda-irisk (referred to here as irisk), a web-based quantitative risk assessment system, was developed to meet this need. the modeling tool enables users to assess, compare, and rank the risks posed by multiple food-hazard pairs at ...201323462073
adaptation of listeria monocytogenes in a simulated cheese medium: effects on virulence using the galleria mellonella infection model.the aim of this study was to evaluate the effect of the acid and salt adaptation in a cheese-based medium on the virulence potential of listeria monocytogenes strains isolated from cheese and dairy processing environment using the galleria mellonella model. four l. monocytogenes strains were exposed to a cheese-based medium in conditions of induction of an acid tolerance response and osmotolerance response (ph 5·5 and 3·5% w/v nacl) and injected in g. mellonella insects. the survival of insects ...201323461530
listeria monocytogenes alters mast cell phenotype, mediator and osteopontin secretion in a listeriolysin-dependent manner.whilst mast cells participate in the immune defence against the intracellular bacterium listeria monocytogenes, there is conflicting evidence regarding the ability of l. monocytogenes to infect mast cells. it is known that the pore-forming toxin listeriolysin (llo) is important for mast cell activation, degranulation and the release of pro-inflammatory cytokines. mast cells, however, are a potential source of a wide range of cytokines, chemokines and other mediators including osteopontin, which ...201323460827
different assembly of acid and salt tolerance response in two dairy listeria monocytogenes wild strains.a lack on the association between acid tolerance response (atr) and osmotolerance response (otr) among listeria monocytogenes dairy isolates was found. in order to evaluate how wild l. monocytogenes isolates mount tolerance responses under a sub-lethal ph and a low sodium chloride concentration (ph 5.5 and 3.5 % [w/v] nacl), a proteomic approach was used. the atr and otr of two l. monocytogenes cheese dairy isolates (strain t8, serotype 4b and a9, serotype 1/2b or 3b) were determined. the proteo ...201323455450
ruminant rhombencephalitis-associated listeria monocytogenes strains constitute a genetically homogeneous group related to human outbreak strains.listeriosis is a disease that causes significant economic losses at the farm level because of high morbidity and mortality in ruminants. this study was performed to investigate the role of ruminants in the epidemiology of listeriosis in northern italy and the possible association of animal-adapted strains of listeria monocytogenes with strains associated with human disease. twenty ruminant rhombencephalitis isolates previously confirmed as l. monocytogenes by bacteriology and pcr were characteri ...201323455337
characterizing uncertainty when evaluating risk management metrics: risk assessment modeling of listeria monocytogenes contamination in ready-to-eat deli meats.this report illustrates how the uncertainty about food safety metrics may influence the selection of a performance objective (po). to accomplish this goal, we developed a model concerning listeria monocytogenes in ready-to-eat (rte) deli meats. this application used a second order monte carlo model that simulates l. monocytogenes concentrations through a series of steps: the food-processing establishment, transport, retail, the consumer's home and consumption. the model accounted for growth inhi ...201323454818
survival of listeria monocytogenes and salmonella enteritidis in sea bream (sparus aurata) fillets packaged under enriched co2 modified atmospheres.the effect of packaging of fresh sea bream fillets under different modified atmospheres (high levels of co2 combined with n2) on the behaviour of listeria monocytogenes and salmonella enteritidis was studied. two different strains of each pathogen were spread over the sea bream fillets stored at either 0 °c or 4 °c for 16 days under standard supermarket lighting conditions (14 h a day). the atmospheres investigated were 60% co2/40% n2, 70% co2/30% n2, 80% co2/20% n2 and air as control. mesophili ...201323454810
peptide-bacteria interactions using engineered surface-immobilized peptides from class iia bacteriocins.specificity of the class iia bacteriocin leucocin a (leua), an antimicrobial peptide active against gram-positive bacteria, including listeria monocytogenes , is known to be dictated by the c-terminal amphipathic helical region, including the extended hairpin-like structure. however, its specificity when attached to a substrate has not been investigated. exploiting properties of leua, we have synthesized two leua derivatives, which span the amphipathic helical region of the wild-type leua, consi ...201323445325
fate of mycobacterium tuberculosis in peroxidase-loaded resting murine macrophages.myeloperoxidase (mpo), in the presence of hydrogen peroxide and a halide represent an efficient microbicidal mechanism of phagocytic cells. mpo is abundant in neutrophils which also respond to infection by producing large amounts of reactive oxygen species (ros). mpo, ros and halide constitute a very toxic antimicrobial system (called the klebanoff system or ks). resting mature macrophages do not contain granular mpo and thus are unable to kill pathogenic mycobacteria and some other microorganis ...201326785781
proteomic analysis shows that individual listeria monocytogenes strains use different strategies in response to gastric stress.ingestion of contaminated dairy products, in particular soft cheese, is one of the major routes of infection by the human pathogen listeria monocytogenes. during cheese processing, this foodborne pathogen is exposed to sublethal acid and osmotic stress conditions, which may induce tolerance responses and influence subsequent survival in the gastric tract. the aim of the current study was to evaluate the impact on a l. monocytogenes cheese isolate (serotype 4b) and two cheese dairy isolates (t8, ...201323441912
genome sequencing identifies two nearly unchanged strains of persistent listeria monocytogenes isolated at two different fish processing plants sampled 6 years apart.listeria monocytogenes is a food-borne human-pathogenic bacterium that can cause infections with a high mortality rate. it has a remarkable ability to persist in food processing facilities. here we report the genome sequences for two l. monocytogenes strains (n53-1 and la111) that were isolated 6 years apart from two different danish fish processers. both strains are of serotype 1/2a and belong to a highly persistent dna subtype (random amplified polymorphic dna [rapd] type 9). we demonstrate us ...201323435887
food microbe tracker: a web-based tool for storage and comparison of food-associated microbes.large amounts of molecular subtyping information are generated by the private sector, academia, and government agencies. however, use of subtype data is limited by a lack of effective data storage and sharing mechanisms that allow comparison of subtype data from multiple sources. currently available subtype databases are generally limited in scope to a few data types (e.g., mlst. net) or are not publicly available (e.g., pulsenet). we describe the development and initial implementation of food m ...201323433376
tracking cross-contamination transfer dynamics at a mock retail deli market using glogerm.ready-to-eat (rte) deli meats are considered a food at high risk for causing foodborne illness. deli meats are listed as the highest risk rte food vehicle for listeria monocytogenes. cross-contamination in the retail deli market may contribute to spread of pathogens to deli meats. understanding potential cross-contamination pathways is essential for reducing the risk of contaminating various products. the objective of this study was to track cross-contamination pathways through a mock retail del ...201323433375
prevalence and characteristics of listeria monocytogenes in bovine colostrum in japan.this study was conducted to determine the prevalence and characteristics of listeria monocytogenes in bovine colostrum in japan. we collected bovine colostrum samples from 210 dams from 21 dairy farms in hokkaido prefecture (japan) between march and june 2009. l. monocytogenes was detected in samples from 6 (28.6%) of the 21 farms. of the 210 samples, 16 (7.6%) were positive for l. monocytogenes. we recovered 80 l. monocytogenes isolates; 44 (55%) isolates were classified as serotype 1/2b and 36 ...201323433372
kinase suppressor of ras 1 is not required for the generation of regulatory and memory t cells.the mammalian target of rapamycin (mtor) kinase is a critical regulator of the differentiation of helper and regulatory cd4+ t cells, as well as memory cd8+ t cells. in this study, we investigated the role of the erk signaling pathway in regulating mtor activation in t cells. we showed that activation of erk following tcr engagement is required for sustained mtor complex 1 (mtorc1) activation. absence of kinase suppressor of ras 1 (ksr1), a scaffold protein of the erk signaling pathway, or inhib ...201323431403
chorioamnionitis caused by listeria monocytogenes: a case report of ultrasound features of fetal infection.maternal listeriosis is often associated with mild symptoms for the patient, but fetal infection can lead to severe adverse perinatal outcome. the most described antenatal symptoms are reduced fetal movements and an abnormal fetal heart rate trace. we present a case of fetal listeriosis suspected by ultrasound findings of fetal gastrointestinal compromise, neonatal diagnosis and outcome.201323429225
mutants of listeriolysin o for enhanced liposomal delivery of macromolecules.delivery of macromolecules into the cytosolic space of eukaryotic cells is a pressing challenge in biopharmaceutics. macromolecules are often encapsulated into liposomes for protection and improved distribution, but the their size often induces endocytosis of the vehicle at the target site, leading to degradation of the cargo. listeriolysin o is a key virulence factor of listeria monocytogenes that forms pores in the endosomal membrane, ultimately allowing the bacterium to escape into the cytoso ...201323416330
comprehensive update of dalbavancin activity when tested against uncommonly isolated streptococci, corynebacterium spp., listeria monocytogenes, and micrococcus spp. (1357 strains).dalbavancin is an investigational lipoglycopeptide having an extended serum elimination half-life allowing once-weekly dosing. data from testing 1357 strains of uncommonly isolated species expand the dalbavancin spectrum details as follows (mic50/90): β-haemolytic streptococcal serogroups c, f, and g (≤0.03/≤0.03 μg/ml), 7 viridans group of streptococci (≤0.03/≤0.03-0.06 μg/ml), 5 corynebacterium spp. (0.06/0.12 μg/ml), listeria monocytogenes (0.06/0.12 μg/ml), and micrococcus spp. (≤0.03/≤0.03 ...201323414746
a potential new pathway for pd-l1 costimulation of the cd8-t cell response to listeria monocytogenes infection.programmed death ligand-1 (pd-l1) is an important negative regulator of t cell immune responses via interactions with pd-1 and cd80. however, pd-l1 can also act as a positive costimulator, but the relevant counterreceptor is not known. we analyzed the role of pd-l1 in cd8-t cell responses to infection with listeria monocytogenes (lm) or vesicular stomatitis virus (vsv). pd-l1 blockade impaired antigen-specific cd8 effector t cell expansion in response to lm, but not to vsv infection, particularl ...201323409193
complete genome sequence of listeria monocytogenes ll195, a serotype 4b strain from the 1983-1987 listeriosis epidemic in switzerland.the complete genome sequence of listeria monocytogenes ll195, a serotype 4b clinical strain isolated during the 1983-1987 listeriosis epidemic in switzerland, is presented.201323405339
impact of the listeria monocytogenes protein inlc on infection in mice.the bacterial pathogen listeria monocytogenes causes serious food-borne illnesses in pregnant women and the immunocompromised. l. monocytogenes promotes its internalization into host epithelial cells and then uses an f-actin-dependent motility process to spread from infected cells to surrounding healthy cells. in cultured enterocytes, efficient spread of l. monocytogenes requires the secreted bacterial protein inlc. inlc promotes dissemination by physically interacting with and antagonizing the ...201323403554
listeriolysin o as a strong immunogenic molecule for the development of new anti-tumor vaccines.the pore-forming toxin listeriolysin o (llo), which is produced by listeria monocytogenes, mediates bacterial phagosomal escape and facilitates bacterial multiplication during infection. this toxin has recently gained attention because of its confirmed role in the controlled and specific modulation of the immune response. currently, cancer immunotherapies are focused on conquering the immune tolerance induced by poorly immunogenic tumor antigens and eliciting strong, lasting immunological memory ...201323399758
processing of recombinant listeria monocytogenes proteins for mhc class i presentation follows a dedicated, high-efficiency pathway.cd8(+) t lymphocytes recognize short peptides of ∼8-10 aa bound to mhc class i molecules (pmhc) on the surface of apcs. these peptides can be generated from either endogenous proteins synthesized by the biosynthetic machinery of the presenting cell or from exogenously sourced proteins. because much of the research characterizing the mhc class i processing pathway has focused on endogenously synthesized proteins, it is not known whether differences exist in the processing pathway followed by endo ...201323396941
effects of high hydrostatic pressure and varying concentrations of sodium nitrite from traditional and vegetable-based sources on the growth of listeria monocytogenes on ready-to-eat (rte) sliced ham.the objective of this study was to determine the effect the source of added nitrite and high hydrostatic pressure (hhp) had on the growth of listeria monocytogenes on ready-to-eat (rte) sliced ham. use of 600mpa hhp for 3min resulted in an immediate 3.9-4.3log cfu/g reduction in l. monocytogenes numbers, while use of 400mpa hhp (3min) provided less than 1log cfu/g reduction. with the 600mpa hhp treatment, sliced ham with a conventional concentration of sodium nitrite (200ppm) was not different i ...201323391864
monoclonal antibodies recognizing the surface autolysin ispc of listeria monocytogenes serotype 4b: epitope localization, kinetic characterization, and cross-reaction studies.listeria monocytogenes serotype 4b is responsible for a high percentage of fatal cases of food-borne infection. in a previous study, we created 15 monoclonal antibodies (mabs) against a ≈ 77 kda antigen that is associated with the cell surface of live l. monocytogenes serotype 4b cells. here we report an extensive characterization of these mabs to further their development as diagnostic reagents. the ≈ 77 kda target antigen was identified by mass spectrometry and n-terminal sequencing to be ispc ...201323390515
improving efficiency of viability-pcr for selective detection of live cells.viability pcr (v-pcr) as a method to selectively detect intact live cells has gained considerable interest over the last years with an increasing number of applications. the principle is based on treatment of microbiological samples with a viability dye prior to extraction of genomic dna and its amplification. the dye is selectively taken up by membrane-compromised dead cells resulting in the degradation of their dna upon light exposure and therefore inhibition of amplification. although the tre ...201323389080
sculpting mhc class ii-restricted self and non-self peptidome by the class i ag-processing machinery and its impact on th-cell responses.it is generally assumed that the mhc class i antigen (ag)-processing (cap) machinery - which supplies peptides for presentation by class i molecules - plays no role in class ii-restricted presentation of cytoplasmic ags. in striking contrast to this assumption, we previously reported that proteasome inhibition, tap deficiency or eraap deficiency led to dramatically altered t helper (th)-cell responses to allograft (hy) and microbial (listeria monocytogenes) ags. herein, we tested whether altered ...201323386199
a defined, glucose-limited mineral medium for the cultivation of listeria spp.members of the genus listeria are fastidious bacteria with respect to their nutritional requirements, and several minimal media described in the literature fail to support growth of all listeria spp. furthermore, strict limitation by a single nutrient, e.g., the carbon source, has not been demonstrated for any of the published minimal media. this is an important prerequisite for defined studies of growth and physiology, including "omics." based on a theoretical analysis of previously published m ...201323377938
genetic determinants for cadmium and arsenic resistance among listeria monocytogenes serotype 4b isolates from sporadic human listeriosis patients.in listeria monocytogenes serotype 4b isolates from sporadic listeriosis, heavy metal resistance was primarily encountered in certain clonal groups (eci, ecii, and ecia). all arsenic-resistant isolates harbored the arsenic resistance cassette previously identified in pli100; ecia harbored additional arsenic resistance genes and a novel cadmium resistance determinant in a conserved chromosomal locus.201323377929
the surface proteins inla and inlb are interdependently required for polar basolateral invasion by listeria monocytogenes in a human model of the blood-cerebrospinal fluid barrier.the gram-positive bacterium listeria monocytogenes can enter the human central nervous system and cause life-threatening meningitis. during this process the pathogen has to invade and cross diverse cellular barriers involving the functions of the surface proteins internalin (inla) and inlb. whereas the internalin-dependent crossing of the intestinal epithelium and the fetoplacental barrier have been subject to intensive investigation, limited research elucidating the crossing of the human blood- ...201323376167
in vivo activity of nisin a and nisin v against listeria monocytogenes in mice.lantibiotics are post-translationally modified antimicrobial peptides, of which nisin a is the most extensively studied example. bioengineering of nisin a has resulted in the generation of derivatives with increased in vitro potency against gram-positive bacteria. of these, nisin v (containing a met21val change) is noteworthy by virtue of exhibiting enhanced antimicrobial efficacy against a wide range of clinical and food-borne pathogens, including listeria monocytogenes. however, this increased ...201323374279
antibacterial effect of light emitting diodes of visible wavelengths on selected foodborne pathogens at different illumination temperatures.the antibacterial effect of light emitting diodes (leds) in the visible region (461, 521 and 642 nm) of the electromagnetic spectrum was investigated on escherichia coli o157:h7, salmonella typhimurium, listeria monocytogenes and staphylococcus aureus. the irradiances of the 461, 521 and 642 nm leds were 22.1, 16 and 25.4 mw/cm², respectively. bacterial cultures suspended in tryptic soy broth were illuminated by 10-watt leds at a distance of 4.5 cm for 7.5h at 20, 15 and 10 °c. regardless of the ...201324026011
survival of foodborne pathogens on inshell walnuts.the survival of salmonella enterica enteritidis pt 30 or five-strain cocktails of s. enterica, escherichia coli o157:h7, and listeria monocytogenes was evaluated on inshell walnuts during storage. inshell walnuts were separately inoculated with an aqueous preparation of the pathogens at levels of 10 to 4 log cfu/nut, dried for 24 h, and then stored at either 4 °c or ambient conditions (23-25 °c, 25-35% relative humidity) for 3 weeks to more than 1 year. during the initial 24-h drying period, bac ...201324026009
effect of various conditions on inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in fresh-cut lettuce using ultraviolet radiation.the effect of various conditions on inactivation of foodborne pathogens and quality of fresh-cut lettuce during ultraviolet (254 nm, uvc) radiation was investigated. lettuce was inoculated with a cocktail of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes and treated at different temperatures (4 and 25 °c), distances between sample and lamp (10 and 50 cm), type of exposure (illuminated from one or two sides), uv intensities (1.36 to 6.80 mw/cm²), and exposure times ( ...201324021819
extent and mode of action of cationic legume proteins against listeria monocytogenes and salmonella enteritidis.the methylated soybean protein and methylated chickpea protein (msp and mcp) with isoelectric points around pi 8 were prepared by esterifying 83 % of their free carboxyl groups and tested for their interactions with listeria monocytogenes and salmonella enteritidis. the two substances exhibited a concentration-dependent inhibitory action against the two studied bacteria with a minimum inhibitory concentration of about 100 μg/ml. the ic50 % of the two proteins against l. monocytogenes (17 μg/ml) ...201326782988
effect of bacteriocins and conditions that mimic food and digestive tract on biofilm formation, in vitro invasion of eukaryotic cells and internalin gene expression by listeria monocytogenes.in this report, listeria monocytogenes isolates were evaluated for their ability to form biofilms, for adhesion/invasion of eukaryotic cells and for differential expression of internalin a (inl a) gene, which is related to virulence potential. the presence of bacteriocins of lactic acid bacteria and incubation at 5 °c were the main factors that influenced biofilm formation by l. monocytogenes, in comparison with bhi (control). in general, adhesion and invasion of caco-2 cells were significantly ...201326782984
occurrence of genetic variants of listeria monocytogenes strains.isolates of listeria monocytogenes saved from outbreaks of listeriosis, cases of sporadic listeriosis, and similar events do not always belong to a solitary genetic variant. variants of the same strain may have evolved from a unique clone, and plasmid loss or gain and phage-mediated genetic changes are suggested as the main mechanism. some of these reports are summarized in this short communication.201323988078
influence of freezing stress on morphological alteration and biofilm formation by listeria monocytogenes: relationship with cell surface hydrophobicity and membrane fluidity.the morphological changes and adhesive property of three listeria monocytogenes strains submitted to freezing stress (-20 °c) were studied. the atomic force micrographs showed a reduction in the cell size and an evolution to coccoid shape. the phenotypic slime production of l. monocytogenes and the expression of the adhesive gene were investigated before and after 10 months of incubation in salmon at -20°. our results showed that after ten months, stressed stains become more adherent and able to ...201323982847
cpg oligodeoxynucleotide induces bone marrow precursor cells into myeloid-derived suppressor cells.dendritic cells (dcs) and myeloid-derived suppressor cells (mdscs) perform a number of functions in different immunological settings. in standard in vitro experiments, dcs are produced from mouse bone marrow (bm) cells in the presence of granulocyte-macrophage colony-stimulating factor (gm-csf) and il-4. our previous study demonstrated that bm precursor cells could differentiate into mdscs when co-cultured with poly (i:c). in the present study, bm precursor cells cultured in gm-csf and il-4 were ...201323982226
the transcription factor interferon regulatory factor 4 is required for the generation of protective effector cd8+ t cells.robust cytotoxic cd8(+) t-cell response is important for immunity to intracellular pathogens. here, we show that the transcription factor ifn regulatory factor 4 (irf4) is crucial for the protective cd8(+) t-cell response to the intracellular bacterium listeria monocytogenes. irf4-deficient (irf4(-/-)) mice could not clear l. monocytogenes infection and generated decreased numbers of l. monocytogenes-specific cd8(+) t cells with impaired effector phenotype and function. transfer of wild-type cd8 ...201323980171
cd38 controls the innate immune response against listeria monocytogenes.cd38, adenosine-5'-diphosphate-ribosyl cyclase 1, is a multifunctional enzyme, expressed on a wide variety of cell types. cd38 has been assigned diverse functions, including generation of calcium-mobilizing metabolites, cell activation, and chemotaxis. using a murine listeria monocytogenes infection model, we found that cd38 knockout (ko) mice were highly susceptible to infection. enhanced susceptibility was already evident within 3 days of infection, suggesting a function of cd38 in the innate ...201323980105
transcription factor σb plays an important role in the production of extracellular membrane-derived vesicles in listeria monocytogenes.gram-negative bacteria produce extracellular outer membrane vesicles (omvs) that interact with host cells. unlike gram-negative bacteria, less is known about the production and role of extracellular membrane vesicles (mvs) in gram-positive bacteria. the food-borne pathogen listeria monocytogenes can survive under extreme environmental and energy stress conditions and the transcription factor σ(b) is involved in this survival ability. here, we first determined the production of mvs from l. monocy ...201323977379
characterization of a potential listeria monocytogenes virulence factor associated with attachment to fresh produce.a study to determine the attachment of l. monocytogenes serotype 4b strain f2365 on vegetables and fruits was conducted. in an initial study, we screened 32 genes encoding surface proteins and lipases of the strain to find highly expressed genes on lettuce leaves. the results showed that transcription levels of lmof2365_0413, lmof2365_0498, lmof2365_0859, lmof2365_2052, and lmof2365_2812 were significantly upregulated on lettuce leaves. in silico analysis showed that lmof2365_0859 contains a put ...201323974144
rhombencephalitis by listeria monocytogenes in a cirrhotic patient: a case report and literature review.we report a case of rhombencephalitis infection by listeria monocytogenes in a 66-year-old man with cirrhosis. the csf analysis indicated l. monocytogenes as the most likely pathogen. blood and csf culture were positive to l. monocytogenes and mri findings were suggestive of rhomboencephalitis. the treatment was started empirically and then modified when the culture results were available. the patient had a full clinical recovery without neurologic sequelae. clinicians should remember that l. mo ...201324018504
listeria monocytogenes strains encoding premature stop codons in inla invade mice and guinea pig fetuses in orally dosed dams.listeria monocytogenes is an important food-borne bacterial pathogen and listeriosis can result in abortions in pregnant women. the bacterium can colonize food-processing environments, where specific molecular subtypes can persist for years. the purpose of this study was to determine the virulence potential of a group of food-processing persistent l. monocytogenes strains encoding a premature stop codon in inla (encoding internalin a) by using two orally dosed models, pregnant mice and pregnant ...201324014646
the transcription factor foxo1 controls central-memory cd8+ t cell responses to infection.memory t cells protect hosts from pathogen reinfection, but how these cells emerge from a pool of antigen-experienced t cells is unclear. here, we show that mice lacking the transcription factor foxo1 in activated cd8+ t cells have defective secondary, but not primary, responses to listeria monocytogenes infection. compared to short-lived effector t cells, memory-precursor t cells expressed higher amounts of foxo1, which promoted their generation and maintenance. chromatin immunoprecipitation se ...201323932570
a role for sirt2-dependent histone h3k18 deacetylation in bacterial infection.pathogens dramatically affect host cell transcription programs for their own profit during infection, but in most cases, the underlying mechanisms remain elusive. we found that during infection with the bacterium listeria monocytogenes, the host deacetylase sirtuin 2 (sirt2) translocates to the nucleus, in a manner dependent on the bacterial factor inlb. sirt2 associates with the transcription start site of a subset of genes repressed during infection and deacetylates histone h3 on lysine 18 (h3 ...201323908241
effectiveness of phages in the decontamination of listeria monocytogenes adhered to clean stainless steel, stainless steel coated with fish protein, and as a biofilm.listeria monocytogenes is a food-borne pathogen which causes listeriosis and is difficult to eradicate from seafood processing environments; therefore, more effective control methods need to be developed. this study investigated the effectiveness of three bacteriophages (limn4l, limn4p and limn17), individually or as a three-phage cocktail at ≈9 log₁₀ pfu/ml, in the lysis of three seafood-borne l. monocytogenes strains (19co9, 19do3 and 19eo3) adhered to a fish broth layer on stainless steel cou ...201323907252
genetic characterization of listeria monocytogenes isolates from food processing facilities before and after postcook chiller heat treatment.possible selection for and establishment of stress-resistant listeria monocytogenes variants as a consequence of heating interventions is of concern to the food industry. lineage analysis and multilocus variable number tandem repeat analysis (mlva) was performed on 20 l. monocytogenes isolates, of which 15 were obtained before and 5 were obtained after heat treatment of a postcook meat chiller. the ctsr gene (a class iii heat shock gene regulator) from 14 isolates was amplified and sequenced bec ...201323905808
commercial biopreservatives combined with salt and sugar to control listeria monocytogenes during smoked salmon processing.three commercial antimicrobials, applied during the salting stage in the preparation of cold-smoked salmon, were investigated for their effect on the behavior of listeria monocytogenes. fresh salmon inoculated with l. monocytogenes inia 2530 was treated with three bacteriocin-based commercial biopreservatives, which were applied in combination with a salt-sugar mix. the product was kept at 8°c for 7 days. l. monocytogenes grew by approximately 3 log cfu/g in control salmon (without the salt-suga ...201323905807
inactivation of human pathogens during phase ii composting of manure-based mushroom growth substrate.commercial production of white button mushrooms (agaricus bisporus) requires a specialized growth substrate prepared from composted agricultural by-products. because horse and poultry manures are widely used in substrate formulations, there is a need to determine the extent to which the composting process is capable of eliminating human pathogens. in this study, partially composted substrate was inoculated with a pathogen cocktail (log 10⁶ to 10⁸ cfu/g) containing listeria monocytogenes, escheri ...201323905795
inhibitory effects of chitosan coating combined with organic acids on listeria monocytogenes in refrigerated ready-to-eat shrimps.chitosan and organic acids are natural preservatives with proven antimicrobial efficacies. this study investigated the effects of chitosan coating combined with organic acids on inhibiting listeria monocytogenes in ready-to-eat (rte) shrimps during storage at 4°c. cooked shrimps inoculated with l. monocytogenes were coated with 0.5 and 1 % chitosan solutions in combination with 2 % organic acids (acetic, citric, lactic, and malic acids) for 5 min and then stored at 4°c for 16 days. bacterial cou ...201323905793
identifying ingredients that delay outgrowth of listeria monocytogenes in natural, organic, and clean-label ready-to-eat meat and poultry products.the objective of this study was to identify ingredients that inhibit listeria monocytogenes in natural, organic, or clean-label ready-to-eat meat and poultry products. fourteen ingredients were screened in uncured (no-nitrate-or-nitrite-added), traditional-cured (156 ppm of purified sodium nitrite), cultured (alternative cured, natural nitrate source, and staphylococcus carnosus), or preconverted (alternative cured, natural nitrite source) turkey slurries. slurries were cooked, cooled, inoculate ...201323905792
fate of naturally occurring escherichia coli o157:h7 and other zoonotic pathogens during minimally managed bovine feedlot manure composting processes.reducing escherichia coli o157:h7 in livestock manures before application to cropland is critical for reducing the risk of foodborne illness associated with produce. our objective was to determine the fate of naturally occurring e. coli o157:h7 and other pathogens during minimally managed on-farm bovine manure composting processes. feedlot pen samples were screened to identify e. coli o157:h7-positive manure. using this manure, four piles of each of three different composting formats were constr ...201323905785
staphylococcus aureus but not listeria monocytogenes adapt to triclosan and adaptation correlates with increased fabi expression and agr deficiency.the ability of pathogens to adapt to the widely used biocide, triclosan, varies substantially. the purpose of the study was to examine bacterial adaptation over an extended period of time to low increments of triclosan concentrations. focus was two human pathogens, s. aureus and l. monocytogenes that previously have displayed inherent high and low adaptability, respectively.201323898801
vision loss following intraocular listeriosis associated with contaminated cantaloupe.intraocular listeriosis, a rare manifestation of invasive listeriosis, has a poor visual prognosis. we report an intraocular listeriosis case related to a multistate outbreak associated with contaminated cantaloupe. increasing awareness of rare listeriosis presentations might facilitate timely diagnosis and treatment, and case reporting can clarify medical and epidemiologic aspects of listeriosis.201323898285
virtual memory cd8 t cells display unique functional properties.previous studies revealed the existence of foreign antigen-specific memory phenotype cd8 t cells in unimmunized mice. considerable evidence suggests this population, termed "virtual memory" (vm) cd8 t cells, arise via physiological homeostatic mechanisms. however, the antigen-specific function of vm cells is poorly characterized, and hence their potential contribution to immune responses against pathogens is unclear. here we show that naturally occurring, polyclonal vm cells have unique function ...201323898211
[neurological complications during treatment of the tumor necrosis alpha inhibitors].medications with tnf-alpha inhibitors family are successfully applicable in rheumatology, gastroenterology, dermatology and neurology. still, the ongoing research on the safety assessment of their application, also due to neurological complications. the vast majority of these complications is associated with an increased risk of serious virus (herpes simplex--jc) and bacterial (listeria monocytogenes) neuroinfections. they can cause the occurrence of progressive multifocal leukoencephalopathy--p ...201323894783
role of a gntr-family response regulator lbra in listeria monocytogenes biofilm formation.the formation of listeria monocytogenes biofilms contributes to persistent contamination in food processing facilities. a microarray comparison of l. monocytogenes between the transcriptome of the strong biofilm forming strain (bfm(s)) scott a and the weak biofilm forming (bfm(w)) strain f2365 was conducted to identify genes potentially involved in biofilm formation. among 951 genes with significant difference in expression between the two strains, a gntr-family response regulator encoding gene ...201323894658
conservation and distribution of the benzalkonium chloride resistance cassette bcrabc in listeria monocytogenes.analysis of a panel of 116 listeria monocytogenes strains of diverse serotypes and sources (clinical, environment of food processing plants, and food) revealed that all but one of the 71 benzalkonium chloride-resistant (bc(r)) isolates harbored bcrabc, previously identified on a large plasmid (plm80) of the 1998-1999 hot dog outbreak strain h7858. in contrast, bcrabc was not detected among bc-susceptible (bc(s)) isolates. the bcrabc sequences were highly conserved among strains of different sero ...201323892748
comparison of listeria monocytogenes exoproteomes from biofilm and planktonic state: lmo2504, a protein associated with biofilms.the food-borne pathogen listeria monocytogenes is the causative agent of the severe human and animal disease listeriosis. the persistence of this bacterium in food processing environments is mainly attributed to its ability to form biofilms. the search for proteins associated with biofilm formation is an issue of great interest, with most studies targeting the whole bacterial proteome. nevertheless, exoproteins constitute an important class of molecules participating in various physiological pro ...201323892746
fluorescent labeling of nisin z and assessment of anti-listerial action.biomolecule labeling by fluorescent markers has emerged as an innovative methodology for bio-analytical purposes in food microbiology, medicine and pharmaceutics due to the great advantages of this method such as precision, wide detection limits, and in vivo recognition. fluorescent nisin z was synthesized by linking the carboxyl group and amino group of nisin z and 5-aminoacetamido fluorescein (aaa-flu). this new structure was fully characterized by mass spectrometry with a molecular weight of ...201323892059
γδ t cells exhibit multifunctional and protective memory in intestinal tissues.the study of t cell memory and the target of vaccine design have focused on memory subsumed by t cells bearing the αβ t cell receptor. alternatively, γδ t cells are thought to provide rapid immunity, particularly at mucosal borders. here, we have shown that a distinct subset of mucosal γδ t cells mounts an immune response to oral listeria monocytogenes (lm) infection and leads to the development of multifunctional memory t cells capable of simultaneously producing interferon-γ and interleukin-17 ...201323890071
effect of different concentrations of acetic, citric, and propionic acid dipping solutions on bacterial contamination of raw chicken skin.bacterial contamination of raw, processed poultry may include spoilage bacteria and foodborne pathogens. we evaluated different combinations of organic acid (oa) wash solutions for their ability to reduce bacterial contamination of raw chicken skin and to inhibit growth of spoilage bacteria and pathogens on skin during refrigerated storage. in experiment 1, raw chicken skin samples were dipped into a suspension of either 10(8) cfu/ml of salmonella typhimurium, escherichia coli o157:h7, or lister ...201323873572
combining individual-based modeling and food microenvironment descriptions to predict the growth of listeria monocytogenes on smear soft cheese.an individual-based modeling (ibm) approach was developed to describe the behavior of a few listeria monocytogenes cells contaminating smear soft cheese surface. the ibm approach consisted of assessing the stochastic individual behaviors of cells on cheese surfaces and knowing the characteristics of their surrounding microenvironments. we used a microelectrode for ph measurements and micro-osmolality to assess the water activity of cheese microsamples. these measurements revealed a high variabil ...201323872572
dna extraction protocol for rapid pcr detection of pathogenic bacteria.twelve reagents were evaluated to develop a direct dna extraction method suitable for pcr detection of foodborne bacterial pathogens. many reagents exhibited strong pcr inhibition, requiring significant dilution of the extract with a corresponding reduction in sensitivity. most reagents also exhibited much lower recovery of dna from the gram-positive test organism (listeria monocytogenes) than from the gram-negative organism (escherichia coli o157:h7), preventing unbiased detection and quantitat ...201323872000
listeria monocytogenes strains isolated from dry milk samples in mexico: occurrence and antibiotic sensitivity.dry milk is a particular concern in mexico, as approximately 150,000 metric tons of dry milk are imported every year at a cost of around $250 million. dry milk is used to make many products, most of which are dairy products widely distributed among the population covered by welfare programs. the purpose of the study described in this article was to determine the presence of listeria spp. in imported dry milk samples in mexico, and to determine the sensitivity of the listeria monocytogenes isolat ...201324073487
from pasteur to probiotics: a historical overview of cheese and microbes.cheese is a food which has been produced for centuries. while cheese was originally developed as a product which extended the shelf life of milk, over time distinct cheese varieties arose, being shaped by geographic, climate, cultural, and economic factors. global demand for artisan cheeses is creating new economic opportunities. consumers seeking distinctive products with regional flavor, or terroir, are becoming connoisseurs of hand-crafted cheeses with distinctive tastes and character. these ...201326184821
pulsed-field gel electrophoresis proficiency testing trials: toward european harmonization of the typing of food and clinical strains of listeria monocytogenes.the european union reference laboratory for listeria monocytogenes (eurl for lm) coordinates a european network of 35 national reference laboratories (nrls), most of which perform food, environmental, and veterinary lm strain surveillance in their respective countries. the eurl activities resulted in the recent creation of a database (eurl lm db). typing and related epidemiological data submitted to the eurl lm db will be collected and shared by all the nrls. for a given nrl, the only criterion ...201324066940
roles of a novel crp/fnr family transcription factor lmo0753 in soil survival, biofilm production and surface attachment to fresh produce of listeria monocytogenes.listeria monocytogenes is a foodborne bacterial pathogen and the causative agent of an infectious disease, listeriosis. l. monocytogenes is ubiquitous in nature and has the ability to persist in food processing environments for extended periods of time by forming biofilms and resisting industrial sanitization. human listeriosis outbreaks are commonly linked to contaminated dairy products, ready-to-eat meats, and in recent years, fresh produce such as lettuce and cantaloupes. we identified a puta ...201324066185
survival of bactericidal antibiotic treatment by a persister subpopulation of listeria monocytogenes.listeria monocytogenes can cause the serious infection listeriosis, which despite antibiotic treatment has a high mortality. understanding the response of l. monocytogenes to antibiotic exposure is therefore important to ensure treatment success. some bacteria survive antibiotic treatment by formation of persisters, which are a dormant antibiotic-tolerant subpopulation. the purpose of this study was to determine whether l. monocytogenes can form persisters and how bacterial physiology affects th ...201324056460
listeria monocytogenes multidrug resistance transporters and cyclic di-amp, which contribute to type i interferon induction, play a role in cell wall stress.the intracellular bacterial pathogen listeria monocytogenes activates a robust type i interferon response upon infection. this response is partially dependent on the multidrug resistance (mdr) transporter mdrm and relies on cyclic-di-amp (c-di-amp) secretion, yet the functions of mdrm and cyclic-di-amp that lead to this response are unknown. here we report that it is not mdrm alone but a cohort of mdr transporters that together contribute to type i interferon induction during infection. in a sea ...201324056102
superior induction and maintenance of protective cd8 t cells in mice infected with mouse cytomegalovirus vector expressing rae-1γ.due to a unique pattern of cd8 t-cell response induced by cytomegaloviruses (cmvs), live attenuated cmvs are attractive candidates for vaccine vectors for a number of clinically relevant infections and tumors. nkg2d is one of the most important activating nk cell receptors that plays a role in costimulation of cd8 t cells. here we demonstrate that the expression of cd8 t-cell epitope of listeria monocytogenes by a recombinant mouse cmv (mcmv) expressing the nkg2d ligand retinoic acid early-induc ...201324052528
isolation and quantification of highly acid resistant variants of listeria monocytogenes.heterogeneity in stress response of bacteria is one of the biggest challenges posed by minimal processing, which aims at finding the balance between microbiologically stable foods while maintaining the characteristics of fresh products. in this study, exposure of listeria monocytogenes lo28 to acid stress, which can be encountered in the food processing environment as well as in the human body upon ingestion, led to inactivation kinetics showing considerable tailing, which was described by a bip ...201324051176
listeria monocytogenes brain abscess: two cases and review of the literature.listeria monocytogenes is a gram-positive bacillus that exhibits predilection to infect the central nervous system in immunocompromised individuals; the most common manifestations are meningitis and rhombencephalitis. listerial brain abscesses are rare. we report here two brain abscess cases caused by l. monocytogenes in patients receiving immunosuppressive agents. the first patient presented with left hemiparesis mimicking stroke and the second patient presented with neurological symptoms witho ...201324050079
identifying the initiating events of anti-listeria responses using mice with conditional loss of ifn-γ receptor subunit 1 (ifngr1).although ifn-γ is required for resolution of listeria monocytogenes infection, the identities of the ifn-γ-responsive cells that initiate the process remain unclear. we addressed this question using novel mice with conditional loss of ifn-γr (ifngr1). itgax-cre(+)ifngr1(f/f) mice with selective ifn-γ unresponsiveness in cd8α(+) dendritic cells displayed increased susceptibility to infection. this phenotype was due to the inability of ifn-γ-unresponsive cd8α(+) dendritic cells to produce the init ...201324048899
Refresh
firstprev
...131132133134135136137138139140...
nextlast
Displaying items 13801 - 13900 of 16792