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requirement of the crors two-component system for resistance to cell-wall-targeting antimicrobials in enterococcus faecium.enterococci are serious opportunistic pathogens that are resistant to many cell-wall-targeting antibiotics. the crors two-component signaling system responds to antibiotic-mediated cell-wall stress and is critical for resistance to cell-wall-targeting antibiotics in enterococcus faecalis here, we identify and characterize an orthologous two-component system found in enterococcus faecium that is functionally equivalent to the crors system of e. faecalis deletion of crors in e. faecium resulted in ...201728223383
comparison of methods for identifying causative bacterial microorganisms in presumed acute endophthalmitis: conventional culture, blood culture, and pcr.identification of bacterial pathogens in endophthalmitis is important to inform antibiotic selection and treatment decisions. hemoculture bottles and polymerase chain reaction (pcr) analysis have been proposed to offer good detection sensitivity. this study compared the sensitivity and accuracy of a blood culture system, a pcr approach, and conventional culture methods for identification of causative bacteria in cases of acute endophthalmitis.201728222703
technological properties and biogenic amines production by bacteriocinogenic lactococci and enterococci strains isolated from raw goat's milk.technological properties and biogenic amine production were analyzed in 56 bacteriocinogenic lactococci and enterococci strains isolated from raw goat's milk. fifteen lactococci strains were able to reduce milk ph to 5.3 or lower after 6 h, while enterococci strains were initially slow in producing acids. lactococcus lactis subsp. lactis glc06 and three strains of enterococcus faecalis (gen20, gen22, and gen23) presented high proteolytic activity. l. lactis subsp. lactis glc06 and e. faecalis ge ...201728221886
conjugative type iv secretion in gram-positive pathogens: trag, a lytic transglycosylase and endopeptidase, interacts with translocation channel protein tram.conjugative transfer plays a major role in the transmission of antibiotic resistance in bacteria. pip501 is a gram-positive conjugative model plasmid with the broadest transfer host-range known so far and is frequently found in enterococcus faecalis and enterococcus faecium clinical isolates. the pip501 type iv secretion system is encoded by 15 transfer genes. in this work, we focus on the virb1-like protein trag, a modular peptidoglycan metabolizing enzyme, and the virb8-homolog tram, a potenti ...201728219792
genetic relatedness of faecal coliforms and enterococci bacteria isolated from water and sediments of the apies river, gauteng, south africa.to date, the microbiological quality of river sediments and its impact on water resources are not included in the water quality monitoring assessment. therefore, the aim of this study was to establish genetic relatedness between faecal coliforms and enterococci isolated from the river water and riverbed sediments of apies river to better understand the genetic similarity of microorganisms between the sediment and water phases. indicator bacteria were subjected to a molecular study, which consist ...201728063147
fosfomycin: mechanism and resistance.fosfomycin, a natural product antibiotic, has been in use for >20 years in spain, germany, france, japan, brazil, and south africa for urinary tract infections (utis) and other indications and was registered in the united states for the oral treatment of uncomplicated utis because of enterococcus faecalis and escherichia coli in 1996. it has a broad spectrum, is bactericidal, has very low toxicity, and acts as a time-dependent inhibitor of the mura enzyme, which catalyzes the first committed ste ...201728062557
critical role of tedizolid in the treatment of acute bacterial skin and skin structure infections.tedizolid phosphate has high activity against the gram-positive microorganisms mainly involved in acute bacterial skin and skin structure infections, such as strains of staphylococcus aureus (including methicillin-resistant s. aureus strains and methicillin-sensitive s. aureus strains), streptococcus pyogenes, streptococcus agalactiae, the streptococcus anginosus group, and enterococcus faecalis, including those with some mechanism of resistance limiting the use of linezolid. the area under the ...201728053508
isolation of rhamnolipids-producing cultures from faeces: influence of interspecies communication on the yield of rhamnolipid congeners.the aim of this study was to evaluate the ability of bacterial cultures isolated from cattle, poultry or pig faeces and manure to produce rhamnolipids, as well as to investigate the influence of interspecies communication on possible quantitative differences in the production of rhamnolipid congeners. initial screening methods (oil spreading, drop collapse, haemolytic activity and emulsification activity) showed that approximately 36% of the 51 isolated cultures exhibited the ability to produce ...201728043869
quantification of the d-ala-d-lac-terminated peptidoglycan structure in vancomycin-resistant enterococcus faecalis using a combined solid-state nuclear magnetic resonance and mass spectrometry analysis.induction of vancomycin resistance in vancomycin-resistant enterococci (vre) involves replacement of the d-ala-d-ala terminus of peptidoglycan (pg) stems with d-ala-d-lac, dramatically reducing the binding affinity of vancomycin for lipid ii. effects from vancomycin resistance induction in enterococcus faecalis (atcc 51299) were characterized using a combined solid-state nuclear magnetic resonance (nmr) and liquid chromatography-mass spectrometry (lc-ms) analysis. solid-state nmr directly measur ...201728040891
a comparison between effect of photodynamic therapy by led and calcium hydroxide therapy for root canal disinfection against enterococcus faecalis: a randomized controlled trial.insufficient root canal disinfection is one of the main reasons for persistent periapical pathology. photodynamic therapy (pdt) has been proven effective in disinfecting infected root canals. the aim of this study was to evaluate the antimicrobial effect of photo activated disinfection (pad) when using toluidine blue as photosensitizer and a led lamp after the conventional treatment, and comparing it with calcium hydroxide therapy in vivo.201728040502
novel lnu(g) gene conferring resistance to lincomycin by nucleotidylation, located on tn6260 from enterococcus faecalis e531.to identify a novel putative lincosamide resistance gene determinant in a swine enterococcus faecalis e531 exhibiting a lincosamide resistance/macrolide susceptibility (l r m s ) phenotype and to determine its location and genetic environment.201728039271
genome sequence of enterococcus faecalis strain cg_e.enterococcus faecalis cg_e is a gram-positive, lactic acid-producing coccus. the draft genome of e. faecalis strain cg_e comprises 2,969,881 bp and exhibits a g+c content of 37.34%. the genome encodes 2,848 predicted protein-encoding and 97 rna genes.201728082508
[early results of adjuvant topical treatment of recurrent osteomyelitis with absorbable antibiotic carriers].background treatment of musculoskeletal infections principally consists of radical surgical debridement and systemic administration of antibiotics. additional local antibiotic therapy is not yet generally established, and lacks evidence-based proof of efficacy. nonetheless, there are a variety of practical approaches, as most specialised departments are unwilling to forego this option. the established polymethylmetacrylate (pmma) carrier system has a number of practical disadvantages. this has l ...201728081583
characterization of multi-drug resistant enterococcus faecalis isolated from cephalic recording chambers in research macaques (macaca spp.).nonhuman primates are commonly used for cognitive neuroscience research and often surgically implanted with cephalic recording chambers for electrophysiological recording. aerobic bacterial cultures from 25 macaques identified 72 bacterial isolates, including 15 enterococcus faecalis isolates. the e. faecalis isolates displayed multi-drug resistant phenotypes, with resistance to ciprofloxacin, enrofloxacin, trimethoprim-sulfamethoxazole, tetracycline, chloramphenicol, bacitracin, and erythromyci ...201728081148
ozone therapy as an adjuvant for endondontic protocols: microbiological - ex vivo study and citotoxicity analyses.this study evaluated the antimicrobial efficacy of ozone therapy in teeth contaminated with pseudomonas aeruginosa, enterococcus faecalis, and staphylococcus aureus using a mono-species biofilm model. parallel to this, the study aimed to evaluate the cytotoxicity of ozone for human gingival fibroblasts. material and methods: one hundred and eighty single-root teeth were contaminated with a mono-species biofilm of enterococcus faecalis, pseudomonas aeruginosa, and staphylococcus aureus. groups we ...201728076466
effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment.the antimicrobial effect of ultrasonic agitation of calcium hydroxide (ch) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (clsm) and microbiological culture.201728076462
a novel phosphodiesterase of the gdpp family modulates cyclic di-amp levels in response to cell membrane stress in daptomycin-resistant enterococci.substitutions in the liafsr membrane stress pathway are frequently associated with the emergence of antimicrobial peptide resistance in both enterococcus faecalis and enterococcus faecium cyclic di-amp (c-di-amp) is an important signal molecule that affects many aspects of bacterial physiology, including stress responses. we have previously identified a mutation in a gene (designated yybt) in e. faecalis that was associated with the development of daptomycin resistance, resulting in a change at ...201728069645
probiotic e. coli nissle 1917 biofilms on silicone substrates for bacterial interference against pathogen colonization.bacterial interference is an alternative strategy to fight against device-associated bacterial infections. pursuing this strategy, a non-pathogenic bacterial biofilm is used as a live, protective barrier to fence off pathogen colonization. in this work, biofilms formed by probiotic escherichia coli strain nissle 1917 (ecn) are investigated for their potential for long-term bacterial interference against infections associated with silicone-based urinary catheters and indwelling catheters used in ...201728069496
design, synthesis and antibacterial evaluation of novel pleuromutilin derivatives possessing piperazine linker.a series of pleuromutilin derivatives bearing piperazine ring have been reported. the in vitro antibacterial activities of the synthetic derivatives against mrsa (atcc 43300), staphylococcus aureus (atcc 29213), enterococcus faecalis (atcc 29212), enterococcus faecium (atcc35667) and escherichia coli (atcc25922) were evaluated by the broth dilution method. most of the synthesized derivatives displayed potent activities. compounds 11c, 12a and 12c were found to be the most active antibacterial de ...201728068600
the effect of diode laser on planktonic enterococcus faecalis in infected root canals in an ex vivo model.this study examined the bactericidal effect of diode laser irradiation against intracanal enterococcus faecalis.201728068207
bactericidal efficacy of photodynamic therapy and chitosan in root canals experimentally infected with enterococcus faecalis: an in vitro study.to evaluate the antibacterial efficacy of photodynamic therapy (pdt) and chitosan against enterococcus faecalis and assess the possible enhancive effect of chitosan on the photosensitizer methylene blue in experimentally infected root canals of extracted human teeth in vitro.201728068186
multidrug-resistant enterococcal infections: new compounds, novel antimicrobial therapies?over the past two decades infections due to antibiotic-resistant bacteria have escalated world-wide, affecting patient morbidity, mortality, and health care costs. among these bacteria, enterococcus faecium and enterococcus faecalis represent opportunistic nosocomial pathogens that cause difficult-to-treat infections because of intrinsic and acquired resistance to a plethora of antibiotics. in recent years, a number of novel antimicrobial compound classes have been discovered and developed that ...201728209400
early in vitro development of daptomycin non-susceptibility in high-level aminoglycoside-resistant enterococcus faecalis predicts the efficacy of the combination of high-dose daptomycin plus ampicillin in an in vivo model of experimental endocarditis. 201728204495
some technological properties of lactic acid bacteria isolated from dahi and datshi, naturally fermented milk products of bhutan.dahi and datshi are common naturally fermented milk (nfm) products of bhutan. population of lactic acid bacteria (lab) in dahi (ph 3.7) and datshi (ph 5.2) was 1.4 × 10(7) and 3.9 × 10(8) cfu/ml, respectively. based on 16s rrna gene sequencing isolates of lab from dahi and datshi were identified as enterococcus faecalis, e. faecium, lactococcus lactis subsp. lactis. lab strains were tested for some technological properties. all lab strains except e. faecalis ch2:17 caused coagulation of milk at ...201728203227
diffusible substances from lactic acid bacterial cultures exert strong inhibitory effects on listeria monocytogenes and salmonella enterica serovar enteritidis in a co-culture model.food-borne infections cause huge economic and human life losses. listeria monocytogenes and salmonella enterica serovar enteritidis are among the top ranking pathogens causing such losses. control of such infections is hampered by persistent contamination of foods and food-processing environments, resistance of pathogens to sanitizing agents, existence of heterogeneous populations of pathogens (including culturable and viable but non-culturable cells) within the same food items, and inability to ...201728202007
effects of lactobacillus casei and enterococcus faecalis on growth performance, immune function and gut microbiota of suckling piglets.this study was carried out to investigate the effects of orally administrated lactobacillus casei and enterococcus faecalis on performance, immune function and gut microbiota of suckling piglets. neonatal piglets (n = 120) were randomly assigned to 4 groups, with 30 suckling piglets in each group. the piglets were from 15 litters, one male and one female piglet were selected for each group in each litter. the control group was administrated with normal saline, the other groups with l. casei or e ...201728201936
linezolid-resistant enterococci in polish hospitals: species, clonality and determinants of linezolid resistance.the significant increase of the linezolid-resistant enterococci (lre) has been observed in polish hospitals since 2012 and our study aimed at elucidating the possible reasons for this phenomenon. polish lre isolates were analysed by multilocus-sequence typing (mlst) and multiple locus variable-number tandem repeat (vntr) analysis (mlva), polymerase chain reaction (pcr) and pcr-restriction fragment length polymorphism (pcr-rflp) to establish clonal relatedness and mechanism of linezolid resistanc ...201728197728
antibacterial effect of urushiol on e. faecalis as a root canal irrigant.the purpose of this study was to compare the antibacterial activity of urushiol against enterococcus faecalis (e. faecalis) to that of naocl.201728194365
is there a place for corticosteroids in the therapy of infective endocarditis? report of a case and review. 201728189738
synthesis and antimicrobial evaluation of cationic low molecular weight amphipathic 1,2,3-triazoles.a library of 28 small cationic 1,4-substituted 1,2,3-triazoles was prepared for studies of antimicrobial activity. the structures addressed the pharmacophore model of small antimicrobial peptides and an amphipathic motif found in marine antimicrobials. eight compounds showed promising antimicrobial activity, of which the most potent compound 10b displayed minimum inhibitory concentrations of 4-8μg/ml against streptococcus agalacticae, staphylococcus aureus, pseudomonas aeruginosa, escherichia co ...201728189422
evaluation of antimicrobial photodynamic therapy using indocyanine green and near-infrared diode laser against enterococcus faecalis in infected human root canals.photodynamic therapy (pdt) is considered a promising adjunct to the currently available endodontic disinfection techniques leading to more effective reduction of intracanal bacteria. the present ex vivo study aimed to assess the antimicrobial effect of pdt using indocyanine green (icg) as photosensitizer and a near-infrared (nir) diode laser in root canals of human teeth infected with enterococcus faecalis.201728186464
effectiveness of repeated photodynamic therapy in the elimination of intracanal enterococcus faecalis biofilm: an in vitro study.the study aimed to investigate the effectiveness of photodynamic therapy in the elimination of intracanal enterococcus faecalis biofilm and to analyse how a repeated light irradiation, replenishment of oxygen and photosensitiser affect the results of the photodynamic disinfecting protocol. after chemomechanical preparation, 46 single-rooted human teeth were infected with a clinical strain of e. faecalis and incubated for a week in microaerobic conditions. the experimental procedures included gro ...201728185089
correlation between infective factors and antibiotic resistance in enterococci clinical isolates in west of iran.the present study was done to scrutinize the possible relation between infective genes and antimicrobial resistance in enterococcus faecalis and enterococcus faecium. considering the fact that the presence of recognized infective determinants among clinical isolates may promote the emergence of infections and persistence of enterococci in hospital settings, which can lead to an increase in antimicrobial resistance. 175 e. faecalis and 67 e. faecium isolated from clinical specimens were used. the ...201728184339
characterization of enterococcus faecalis isolated from the cloaca of "fancy breeds" and confined chickens.the aim was to investigate the intestinal community of e. faecalis from healthy confined non-industrialized chicken.201728177186
enterocin as-48 as evidence for the use of bacteriocins as new leishmanicidal agents.we report the feasibility of enterocin as-48, a circular cationic peptide produced by enterococcus faecalis, as a new leishmanicidal agent. as-48 is lethal to leishmania promastigotes as well as to axenic and intracellular amastigotes at low micromolar concentrations, with scarce cytotoxicity to macrophages. as-48 induced a fast bioenergetic collapse of l. donovani promastigotes but only a partial permeation of their plasma membrane with limited entrance of vital dyes, even at concentrations bey ...201728167557
combination of ceftriaxone and ampicillin for the treatment of enterococcal endocarditis.the aim of this systematic review is to review all human trials assessing the efficacy and safety of ampicillin and ceftriaxone for enterococcal endocarditis and to discuss the clinical implications of the findings.201728166656
nipple shields in transaxillary breast augmentation.infection after breast augmentation occurs in 1.1% to 2.5% of patients. bacterial contamination of the implants could explain some complications of breast implant surgery, including infection, capsular contracture and even anaplastic large cell lymphoma. because of the evidence of bacterial spread from the nipple, nipple shields have been proposed as a routine maneuver to avoid contamination of the implants.201728166134
microbial shifts in the porcine distal gut in response to diets supplemented with enterococcus faecalis as alternatives to antibiotics.gut microbiota plays an important role in host health and nutrient digestion of animals. probiotics have become one of effective alternatives to antibiotics enhancing animal health and performance through modulating gut microbiota. previously, our research demonstrated that dietary enterococcus faecalis uc-100 substituting antibiotics enhanced growth and health of weaned pigs. to investigate the alterations of microbiota in the distal gut of pigs fed e. faecalis uc-100 substituting antibiotics, ...201728165001
screening for antibacterial and antibiofilm activities in astragalus angulosus.in a search for finding novel therapeutic agents, extracts from an endemic lebanese plant, astragalus angulosus, were evaluated for their potential in-vitro antibacterial and antibiofilm activities against three gram-positive bacterial strains; staphylococcus epidermidis (cip444), staphylococcus aureus (atcc25923), and enterococcus faecalis (atcc29212); in addition to two gram-negative strains, escherichia coli (atcc35218) and pseudomonas aeruginosa (atcc27853).201728163960
cold atmospheric pressure plasma elimination of clinically important single- and mixed-species biofilms.mixed-species biofilms reflect the natural environment of many pathogens in clinical settings and are highly resistant to disinfection methods. an indirect cold atmospheric-pressure air-plasma system was evaluated under two different discharge conditions for its ability to kill representative gram-positive (staphylococcus aureus) and gram-negative (pseudomonas aeruginosa) pathogens. plasma treatment of individual 24-h-old biofilms and mixed-species biofilms that contained additional species (ent ...201728161488
in vitro activity of oritavancin against gram-positive pathogens isolated in canadian hospital laboratories from 2011 to 2015.gram-positive bacterial pathogens isolated from patient specimens submitted to 15 canadian hospital laboratories from 2011 to 2015 were tested in the coordinating laboratory for susceptibility to oritavancin and comparative antimicrobial agents using the clinical and laboratory standards institute m07-a10 (2015) broth microdilution method. oritavancin's in vitro activity was equivalent to, or more potent than, vancomycin, daptomycin, linezolid, and tigecycline against methicillin-susceptible sta ...201728159446
synthesis, surfactant properties and antimicrobial activities of methyl glycopyranoside ethers.a series of amphiphilic methyl glucopyranoside ethers incorporating various alkyl chain lengths has been synthesized from commercially available methyl glucopyranosides following an acetalisation/hydrogenolysis sequence. the amphiphilic properties of ethers and acetal intermediates were evaluated. both families exhibit excellent surfactant properties with a maximum efficiency obtained for compounds bearing a linear dodecyl chain (cmc = 0.012 mm, γsat. = 30 mn m(-1)). antimicrobial activity studi ...201728157594
synthesis of novel pyrazinamide derivatives based on 3-chloropyrazine-2-carboxamide and their antimicrobial evaluation.aminodehalogenation of 3-chloropyrazine-2-carboxamide with variously substituted benzylamines yielded a series of fifteen 3-benzylaminopyrazine-2-carboxamides. four compounds possessed in vitro whole cell activity against mycobacterium tuberculosis h37rv that was at least equivalent to that of the standard pyrazinamide. mic values ranged from 6 to 42 μm. the best mic (6 μm) was displayed by 3-[(4-methylbenzyl)amino]pyrazine-2-carboxamide (8) that also showed low cytotoxicity in the hepg2 cell li ...201728157178
readmission rate and causes at 90-day after radical cystectomy in patients on early recovery after surgery protocol.background: radical cystectomy (rc) is associated with high risk of early and late perioperative complications, and readmissions. the enhanced recovery after surgery (eras) protocol has been applied to rc showing decreased hospital stay without increased morbidity. objective: to evaluate the specific causes of hospital readmissions in rc patients treated before and after adoption of an eras protocol at our institution. methods: we retrospectively evaluated the outcome of 207 rc patients on eras ...201728149935
sealing efficacy of mineral trioxide aggregate with and without nanosilver for root end filling: an in vitro bacterial leakage study.various materials have been added to mineral trioxide aggregate to enhance its properties. this study was aimed to compare the sealing efficacy of mta with and without nanosilver using bacterial leakage approach.201728149459
retrospective comparative analysis of cardiovascular implantable electronic device infections with and without the use of antibacterial envelopes.cardiovascular implantable electronic device (cied) infections are associated with morbidity and mortality. peri-operative systemic intravenous antibiotic prophylaxis reduces the rate of cied infections. aigisrx, a polymer envelope implanted with the cied, releases minocycline and rifampin, and has been introduced to reduce infections.201728131641
diversity of enterococcus faecalis genotypes from multiple oral sites associated with endodontic failure using repetitive sequence-based polymerase chain reaction and arbitrarily primed polymerase chain reaction.the aim of this study was to evaluate the diversity and similarity of enterococcus faecalis genotype isolates from multiple oral sites using repetitive sequence-based polymerase chain reaction and arbitrarily primed polymerase chain reaction (ap-pcr).201728131414
[update on antibiotic resistance in gram-positive bacteria].antimicrobial resistance among gram-positive bacteria, especially in staphylococcus aureus, enterococcus faecium, enterococcus faecalis, and streptococcus pneumoniae, is a serious threat to public health. these microorganisms have multiple resistance mechanisms to agents currently used in clinical practice. many of these resistance mechanisms are common to all 4 of these bacterial species, but other mechanisms seem to be more specific. the prevalence and dissemination of these mechanisms varies ...201728129816
antimicrobial activity of endodontic medicaments and vehicles using agar well diffusion method on facultative and obligate anaerobes.the aim of this study was to determine the relative antimicrobial effectiveness of these endodontic medicaments and various vehicles using an agar well diffusion assay.201728127166
a comparison of antibacterial and antibiofilm efficacy of phenothiazinium dyes between gram positive and gram negative bacterial biofilm.antimicrobial photodynamic therapy (apdt) is a process that generates reactive oxygen species (ros) in presence of photosensitizer, visible light and oxygen which destroys the bacterial cells. we investigated the photoinactivation efficiency of phenothiazinium dyes and the effect of ros generation on gram positive and gram negative bacterial cell as well as on biofilm.201728119141
growth, biogenic amine production and tyrdc transcription of enterococcus faecalis in synthetic medium containing defined amino acid concentrations.the tyraminogenic potential of the strains enterococcus faecalis ef37 and atcc 29212 was investigated in a synthetic medium containing defined amounts of tyrosine and phenylalanine at different temperatures.201728117533
catheter-associated urinary tract infection (cauti) after term cesarean delivery: incidence and risk factors at a multi-center academic institution.the purpose of this study is to identify the rate of catheter-associated urinary tract infection (cauti) after cesarean delivery (cd) and to determine if any factors increase risk of infection.201728114875
comparative efficacies of daptomycin, vancomycin, and linezolid in experimental enterococcal peritonitis.enterococci have become increasingly important pathogens for nosocomial infection (e.g. bacteremia, intra-abdominal infection, endocarditis, etc.), related to their intrinsic resistance to many antibiotics. although the in vitro susceptibility of daptomycin (dap) against enterococci is well established, the food and drug administration has only approved its use for complicated skin and skin structure infections induced by enterococcus faecalis. in this study we evaluated the potential therapeuti ...201728108098
antibacterial activity, computational analysis and host toxicity study of thymol-sulfonamide conjugates.methicillin-resistant staphylococcus aureus (mrsa) and vancomycin resistant enterococcus faecalis (vre) are notorious pathogenic multidrug resistant (mdr) bacteria in both hospital and community sectors, and today the first antibacterial drug sulfamethoxazole is ineffective. the monoterpene phenol, thymol was conjugated with seven sulfa drug derivatives individually, adopting the dye-azo synthesis protocol, and conjugates were characterized using spectral analysis techniques such as, uv, ftir, m ...201728107695
draft genome sequence of an enterococcus faecalis atcc 19433 siphovirus isolated from raw domestic sewage.we previously isolated and characterized an enterococcus faecalis atcc 19433 siphovirus from raw domestic sewage as a viral indicator of human fecal pollution. here, we report the draft genome sequence of this bacteriophage.201728104647
model systems for the study of enterococcal colonization and infection.enterococcus faecalis and enterococcus faecium are common inhabitants of the human gastrointestinal tract, as well as frequent opportunistic pathogens. enterococci cause a range of infections including, most frequently, infections of the urinary tract, catheterized urinary tract, bloodstream, wounds and surgical sites, and heart valves in endocarditis. enterococcal infections are often biofilm-associated, polymicrobial in nature, and resistant to antibiotics of last resort. understanding enteroc ...201728102784
interspecies transfer of vancomycin, erythromycin and tetracycline resistance among enterococcus species recovered from agrarian sources.enterococci are now well recognised for their ability to transfer antibiotic resistance and for their association with nosocomial infections, but less is known regarding their relevance in the wider environment. enterococcus faecalis and enterococcus faecium were isolated from a range of agrarian associated sources (low-flow water, septic tank, poultry litter, high flow water, slurry/soil) and were assessed for latent ability to transfer antimicrobial resistance.201728100194
biological activities of undescribed north american lichen species.lichens provide a large array of compounds with the potential for pharmaceutical development. in the present study, extracts from three previously undescribed north american lichen species were examined for antioxidant, antibacterial and anticancer activities.201728369962
antimicrobial activity and chemical composition of "kpètè-kpètè": a starter of benin traditional beer tchoukoutou.the aim of this study was to investigate the antibacterial effect of the crude starter "kpètè-kpètè" and lactic acid bacteria used during the production of "tchoukoutou." to achieve this, a total of 11 lactic acid bacteria and 40 starter samples were collected from four communes. the samples were tested on 29 gram + and - strains by disk diffusion method. the minimum inhibitory and bactericidal concentrations of starter and lactic acid bacteria were determined by conventional methods. organic ac ...201728367445
vitreous hemorrhage as an early sign of acute bacterial endophthalmitis following intravitreal ranibizumab injection.to report a case of acute bacterial endophthalmitis after antivascular endothelial growth factor injection with a rare presentation of vitreous hemorrhage.201728365853
phosphotransferase systems in enterococcus faecalis og1rf enhance anti-stress capacity in vitro and in vivo.phosphotransferase systems are common and essential in bacteria, which are in charge of sugar transportation and phosphorylation. however, phosphotransferase systems were found in recent years to be associated with environmental stress factors. this study investigated the role of the mannose/fructose/sorbose phosphotransferase systems in enterococcus faecalis og1rf in adaption to harsh environments by construction of pts mutants. more than one mannose/fructose/sorbose phosphotransferase system w ...201728365379
[bactericidal effects and smear layer removal of er:yag-laser radiation against enterococcus faecalis in root canals].to evaluate the cleaning ability of er:yag laser against enterococcus faecalis in root canals.
 methods: the single-rooted human teeth were sterilized and inoculated with enterococcus faecalis and were randomly assigned into 2 groups. a group of teeth was irrigated with saline during root canal preparation while another group with naclo. after mechanical preparation, the two groups were randomly divided into 3 subgroups according to the different treatments: samples, laser radiation, and calcium ...201728364108
chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against enterococcus faecalis biofilms.propolis obtained from bee hives is a natural substance with antimicrobial properties. it is limited by its insolubility in aqueous solutions; hence ethanol and ethyl acetate extracts of malaysian propolis were prepared. both the extracts displayed antimicrobial and anti-biofilm properties against enterococcus faecalis, a common bacterium associated with hospital-acquired infections. high performance liquid chromatography (hplc) analysis of propolis revealed the presence of flavonoids like kaemp ...201728362873
rapid molecular identification of fecal origin-colonies growing on enterococcus spp.-specific culture methods.the mei, chromocult(®) enterococci, and m-enterococcus culture-based methods used to assess water quality by the detection of enterococcus spp. were first compared in terms of sensitivity using (1) 41 different type strains of enterococcus spp. and (2) environmental colonies identified by 16s rrna sequencing. then, two specific-rtpcr assays targeting enterococcus spp. and enterococcus faecalis/faecium were tested for their ability to confirm the identity of putative enterococcal colonies. the me ...201728362305
attributable mortality of hospital-acquired bloodstream infections in ireland.to estimate the attributable mortality of hospital-acquired bloodstream infections (ha-bsi) in ireland.201728359546
prevalence and bacterial isolates of mastitis in dairy farms in selected districts of eastern harrarghe zone, eastern ethiopia.the study was conducted from november 2015 to april 2016 to estimate the prevalence of clinical and subclinical mastitis in lactating cows, to assess the associated risk factors, and to isolate the major bacterial pathogens in dairy farms in selected district of eastern harrarghe zone, eastern ethiopia. the study was carried out in 384 dairy cows based on data collection, farm visit, animal examination, california mastitis test (cmt), and isolation bacterial pathogens using standard techniques. ...201728352648
synergistic effect of haloduracin and chloramphenicol against clinically important gram-positive bacteria.the emergence of drug-resistant pathogens has triggered the search for more efficient antimicrobial agents and formulations for treatment of infections. in recent years, combination therapy has become one of the effective clinical practices in treating infections. the present study deals with the effect of haloduracin, a lantibiotic bateriocin and chloramphenicol against clinically important bacteria. the combined use of haloduracin and chloramphenicol resulted in remarkable synergy against a sp ...201728352561
good bugs vs bad bugs: evaluation of inhibitory effect of selected probiotics against enterococcus faecalis.the main goal of endodontics is the prevention of apical periodontitis. this is due to the presence of persistent pathogenic microorganisms, such as enterococcus faecalis, and its ability to directly cause acute and chronic inflammation in the periapical tissues. lactobacillus has been shown to promote health in the intestines as well as to inhibit the growth of certain problematic oral bacteria. this study explores shifting the established paradigm of endodontic treatment, which has focused on ...201728349910
bacterial colonization in the apical part of extracted human teeth following root-end resection and filling: a confocal laser scanning microscopy study.the purpose of this study was to evaluate enterococcus faecalis colonization at the apical part of root canals following root-end resection and filling using confocal laser scanning microscopy (clsm).201728349219
primary pyogenic ventriculitis caused by neisseria meningitidis: case report and review of the literature.background. pyogenic ventriculitis is a well-known complication of meningitis, brain abscesses and intraventricular drains. primary pyogenic ventriculitis is a rare entity and few cases have been described so far. we report the first case of primary pyogenic ventriculitis in an adult caused by neisseria meningitidis and present an overview of all reported adult primary pyogenic ventriculitis cases in the english literature. methods. a pubmed search was performed using the terms ependymitis, vent ...201728348798
in vitro evaluation of the antibacterial activity of five sealers used in root canal therapy.antibacterial activity is one of the important characteristics of an ideal root canal sealer. the aim of this study was to investigate the antibacterial activity of five different sealers against enterococcus faecalis using two different methods.201728348620
characterization of vancomycin-resistant enterococcus faecalis and enterococcus faecium isolated from fresh produces and human fecal samples.increased enterococcal infections in hospitals and multidrug-resistant and vancomycin-resistant enterococci (vre) isolated from humans, animals, and food sources raised public health concern on the presence of vre in multiple sources. we performed a comparative analysis of the antimicrobial resistance and genetics of vre isolates derived from fresh produce and human fecal samples. of 389 enterococcus isolates, 8 fecal and 3 produce isolates were resistant to vancomycin and teicoplanin; all harbo ...201728346839
extraction of chitosan and its oligomers from shrimp shell waste, their characterization and antimicrobial effect.the present study was performed to utilize the shrimp shell waste for chitin and chitosan production, characterization by fourier transform infrared (ft-ir) technique and to evaluate the antimicrobial effects of chitosan oligomers produced by depolymerization of chitosan by nitrous acid.201728344399
distribution of antimicrobial resistance determinants, virulence-associated factors and crispr loci in isolates of enterococcus faecalis from various settings and genetic lineages.enterococcus faecalis represents an important factor of hospital-associated infections (hais). the knowledge on its evolution from a commensal to an opportunistic pathogen is still limited, thus we performed a study to characterize distribution of factors that may contribute to this adaptation. using a collection obtained from various settings (hospitalized patients, community carriers, animals, fresh food, sewage, water) we investigated differences in antimicrobial susceptibility, distribution ...201728334141
cutaneous burn injury modulates urinary antimicrobial peptide responses and the urinary microbiome.characterization of urinary bacterial microbiome and antimicrobial peptides after burn injury to identify potential mechanisms leading to urinary tract infections and associated morbidities in burn patients.201728333758
ecological impact of ciprofloxacin on commensal enterococci in healthy volunteers.the ecological impact of ciprofloxacin on commensal enterococci is unknown.201728333351
high incidence of virulence factors among clinical enterococcus faecalis isolates in southwestern iran.over the past two decades, enterococci have emerged as an important agent responsible for hospital acquired infection. several virulence factors contribute to the adherence, colonization, evasion of the host immune response, and pathogenicity and severity of the infection. enterococcus faecalis is the most common and virulent species causing infections in hospitalized patients. the aim of the present study was to examine the prevalence of genes encoding virulence factors and antimicrobial resist ...201728332345
porphyromonas gingivalis is the most abundant species detected in coronary and femoral arteries.an association between oral bacteria and atherosclerosis has been postulated. a limited number of studies have used 16s rna gene sequencing-based metagenomics approaches to identify bacteria at the species level from atherosclerotic plaques in arterial walls. the objective of this study was to establish detailed oral microbiome profiles, at both genus and species level, of clinically healthy coronary and femoral artery tissues from patients with atherosclerosis. tissue specimens were taken from ...201728326156
first canadian case report of kidney transplantation from an hiv-positive donor to an hiv-positive recipient.kidney transplantation has become standard of care for carefully selected patients living with human immunodeficiency virus (hiv) and end-stage renal disease (esrd) in the highly active antiretroviral therapy (haart) era. american and european prospective cohort studies have reported similar patient and graft survival compared with hiv-negative kidney transplant recipients. despite an increased rate of acute rejection, partially due to drug interactions, hiv immunovirologic parameter generally r ...201728321326
negative allosteric regulation of enterococcus faecalis small alarmone synthetase relq by single-stranded rna.the alarmone nucleotides guanosine pentaphosphate (pppgpp) and tetraphosphate (ppgpp), collectively referred to as (p)ppgpp, are key regulators of bacterial growth, stress adaptation, pathogenicity, and antibiotic tolerance. we show that the tetrameric small alarmone synthetase (sas) relq from the gram-positive pathogen enterococcus faecalis is a sequence-specific rna-binding protein. relq's enzymatic and rna binding activities are subject to intricate allosteric regulation. (p)ppgpp synthesis i ...201728320944
efficacy of telavancin alone and in combination with ampicillin in a rat model of enterococcus faecalis endocarditis.we first assessed telavancin (tlv) pharmacokinetics after a single subcutaneous dose of 35 mg/kg. the pharmacokinetic data were used to predict a tlv dose that simulates human exposure and the efficacy of tlv was then evaluated using tlv 21 mg/kg q 12 h against enterococcus faecalis og1rf (tlv mic of 0.06 μg/ml) in a rat endocarditis model with an indwelling catheter. therapy was given for 3 days with tlv, daptomycin (dap) or ampicillin (amp) monotherapy and with combinations of tlv plus amp, am ...201728320712
effect of using different vehicles on the physicochemical, antimicrobial, and biological properties of white mineral trioxide aggregate.this study evaluated the physicochemical, antimicrobial, and biological properties of white mineral trioxide aggregate (mta) mixed with aqueous and propylene glycol extracts of arctium lappa l. and casearia sylvestris sw.201728320540
biofilm formation capability of enterococcal strains causing urinary tract infection vis-a-vis colonisation and correlation with enterococcal surface protein gene.data regarding differences in biofilm formation among urinary isolates of enterococci causing nosocomial infection versus asymptomatic colonisation is lacking. conflicting data are available for the role of enterococcal surface protein (esp) gene in the development of enterococcal biofilms.201728303818
draft genome sequence of a multidrug-resistant strain of enterococcus faecalis, pm01, isolated from the nest of an american bushtit, psaltriparius minimus.pathogenic microorganisms associated with avian nests may detrimentally impact parental health and nest success for the nest primary users, potentially neighboring avian or terrestrial species, including humans. here, we report the genome sequence of enterococcus faecalis strain pm01, isolated from a failed nest of american bushtits, psaltriparius minimus.201728302771
purulent pericarditis as a complication of bacteraemic enterococcus faecalis urinary tract infection.purulent pericarditis is a rare clinical entity in the modern antibiotic era. the most common portal of entry is thought to be direct extension from a primary lung source and is usually caused by staphylococcus aureus, streptococcus pneumoniae or haemophilus influenzae we report the case of a man aged 69 years who presented with purulent pericarditis due to enterococcus faecalis likely caused by haematogenous spread from a urinary tract source. urgent pericardiocentesis was vital and restored hi ...201728298381
the impact of a 940 nm diode laser with radial firing tip and bare end fiber tip on enterococcus faecalis in the root canal wall dentin of bovine teeth: an in vitro study.this in vitro study aimed to compare the bactericidal effect of two different laser delivery systems, a radial firing tip (rft) and bare end fiber tip (bft) used with the 940 nm diode laser on enterococcus faecalis inoculated onto bovine radicular dentin.201728294701
successful salvage treatment of native valve enterococcus faecalis infective endocarditis with telavancin: two case reports.infective endocarditis (ie) one-year mortality rates approach 40%. here, we report two native valve enterococcus faecalis ie cases in patients successfully treated with telavancin. an 88-year-old with mitral valve endocarditis and a penicillin allergy, initially treated with intravenous vancomycin, was switched to telavancin. a 69-year-old, who previously received amoxicillin and intravenous vancomycin for presumed enterococcal bacteraemia, was diagnosed with dual valve endocarditis for which he ...201728293958
sonication of arthroplasty implants improves accuracy of periprosthetic joint infection cultures.there is evidence that sonication of explanted prosthetic hip and knee arthroplasty components with culture of the sonication fluid may enhance diagnostic sensitivity. previous studies on the use of implant sonicate cultures have evaluated diagnostic thresholds but did not elaborate on the clinical importance of positive implant sonicate cultures in the setting of presumed aseptic revisions and did not utilize consensus statements on periprosthetic joint infection (pji) diagnosis when defining t ...201728290115
antimicrobial properties of copper nanoparticles and amino acid chelated copper nanoparticles produced by using a soya extract.this paper reports a comparison of the antibacterial properties of copper-amino acids chelates and copper nanoparticles against escherichia coli, staphylococcus aureus, and enterococcus faecalis. these copper-amino acids chelates were synthesized by using a soybean aqueous extract and copper nanoparticles were produced using as a starting material the copper-amino acids chelates species. the antibacterial activity of the samples was evaluated by using the standard microdilution method (clsi m100 ...201728286459
fosfomycin and comparator activity against select enterobacteriaceae, pseudomonas, and enterococcus urinary tract infection isolates from the united states in 2012.fosfomycin is a broad-spectrum cell wall active agent that inhibits the mura enzyme involved in peptidoglycan synthesis and is fda-approved for treatment of uncomplicated urinary tract infections (utis) caused by escherichia coli and enterococcus faecalis in women. data regarding the susceptibility of recent uti isolates to fosfomycin are limited.201728285420
detection of novel oxazolidinone and phenicol resistance gene optra in enterococcal isolates from food animals and animal carcasses.altogether 7720 enterococcus faecalis and 3939 e. faecium isolated from food animals and animal carcasses during 2003-2014 in korea were investigated to determine if linezolid-resistant (lr) enterococci (≥8μg/ml) are present. overall, 12 e. faecalis and 27 e. faecium recovered from chickens (n=32), pigs (n=6), and cattle (n=1) were resistant to linezolid and were further characterized using molecular methods most lr isolates were also resistant to chloramphenicol (97.44%) and florfenicol (92.31% ...201728284617
chemical composition, antioxidant and antibacterial activities of tamarix balansae j. gay aerial parts.a previously undescribed phenolic sulphate ester, potassium 34-dihydroxy-3-methoxybenzoic acid methyl ester-5-sulphate (1), along with nine known flavonoids, kaempferol-3-o-potassium sulphate-4',7-dimethyl ether (2), kaempferol-4',7-dimethyl ether (3), rhamnocitrin-3-o-potassium sulphate (4), rhamnocitrin (5), kaempferol (6), quercetin (7), afzelin (8), quercetin-3-o-α-l-rhamnopyranoside (9) and luteolin-3'-o-potassium sulphate (10) were isolated from the aerial parts of tamarix balansae. struct ...201728281364
ultraviolet (uv)-reflective paint with ultraviolet germicidal irradiation (uvgi) improves decontamination of nosocomial bacteria on hospital room surfaces.an ultraviolet germicidal irradiation (uvgi) generator (the torch™, clordisys solutions, inc.) was used to compare the disinfection of surface coupons (plastic from a bedrail, stainless steel, and chrome-plated light switch cover) in a hospital room with walls coated with ultraviolet (uv)-reflective paint (lumacept) or standard paint. each surface coupon was inoculated with methicillin-resistant staphylococcus aureus (mrsa) or vancomycin-resistant enterococcus faecalis (vre), placed at 6 differe ...201728278065
effect of photodynamic therapy in combination with various irrigation protocols on an endodontic multispecies biofilm ex vivo.to analyse the antibacterial effect of photodynamic therapy (pdt) in combination with various irrigation protocols on a multispecies biofilm in root canals ex vivo.201728276583
identification of the cfr methyltransferase gene in enterococcus faecalis isolated from swine: first report in brazil. 201728274847
effectiveness of persea major kopp (lauraceae) extract against enterococcus faecalis: a preliminary in vitro study.persea major kopp (lauraceae) is a plant with wound healing, antibacterial, and analgesic properties. the aim of this study was to assess the in vitro antibacterial activity of the concentrated crude extract (cce) and ethyl acetate fraction (eaf) of this plant against enterococcus faecalis and compare it with calcium hydroxide [ca(oh)2] paste and 2% chlorhexidine digluconate (chx).201728264708
susceptibility to disinfectants in antimicrobial-resistant and -susceptible isolates of escherichia coli, enterococcus faecalis and enterococcus faecium from poultry - esbl / ampc-phenotype of escherichia coli is not associated with resistance to a quaternary ammonium compound, ddac.the spread of bacteria that are simultaneously resistant to disinfectants and antimicrobials would constitute an unsettling scenario. in order to explore an association between antimicrobial resistance and reduced susceptibility to biocides / microbicides like disinfectants in agriculture, we investigated escherichia (e.) coli (n = 438) and enterococci (n = 120) isolated from six different flocks of the same poultry farm with known history of antimicrobial treatment.201728261951
antibacterial efficacy of octenisept, alexidine, chlorhexidine, and sodium hypochlorite against enterococcus faecalis biofilms.the purpose of this study was to evaluate the antibacterial effectiveness of octenisept (oct; schülke & mayr gmbh, norderstedt, germany), 1% alexidine (alx) (santa cruz biotechnology, inc, santa cruz, ca), and 2% chlorhexidine (chx) against enterococcus faecalis biofilm using confocal laser scanning microscopy.201728258812
beneficial and safety properties of a corynebacterium vitaeruminis strain isolated from the cow rumen.corynebacterium vitaeruminis mru4 was isolated from the cow rumen and was differentiated from other isolates by rep-pcr and rapd and identified by 16s rrna sequencing. this strain presented higher survival rates for low ph and bile salts treatments, and it was able to survive and multiply in simulated gastric and intestinal environments. c. vitaeruminis mru4 had a 53.2% auto-aggregation rate, 42.4% co-aggregation rate with listeria monocytogenes scott a, 41.6% co-aggregation rate with enterococc ...201728258546
evaluation of 4% sodium hypochlorite in eliminating enterococcus faecalis from the root canal when used with three irrigation methods: an in vitro study.endodontic treatment removes all pathogens, such as enterococcus faecalis from pulp and root canals. the aim of this study is to assess the usefulness of sodium hypo-chlorite (naocl) in removing e. faecalis from the root canal used with three different irrigation methods.201728258267
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