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[protective effect of the aerosol immunization of mice with the polycomponent vaccine immunovac vp-4 after the challenge of the animals with klebsiella pneumoniae virulent strain].used four schemes of the administration of the preparation with different time of the exposition of the animals in an aerosol chamber were tested with their subsequent intraperitoneal challenge with k. pneumoniae virulent strain k16. irrespective of the number of immunization courses, the administration of the preparation made at intervals of 1 day, or daily, did not ensure any protective effect, but only led to an insignificant increase in their survival time in comparison with nonimmunized ani ...200616532635
clinical and economic impact of bacteremia with extended- spectrum-beta-lactamase-producing enterobacteriaceae.we studied outcomes of extended-spectrum beta-lactamase (esbl) production in enterobacteriaceae bacteremia. inpatients with bacteremia caused by esbl-producing escherichia coli, klebsiella spp., or proteus spp. (cases) were compared with patients with bacteremia caused by non-esbl producers (controls). outcomes included mortality, mortality due to infection, length of stay (los), delay in appropriate therapy (dat), discharge to a chronic care facility, and hospital cost. ninety-nine cases and 99 ...200616569837
in vitro antibacterial, antifungal & cytotoxic activity of some isonicotinoylhydrazide schiff's bases and their cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes.isonicotinoylhydrazide schiff's bases formed by the reaction of substituted and unsubstituted furyl-2-carboxaldehyde and thiophene-2-carboxaldehyde with isoniazid and, their co (ii), cu (ii), ni (ii) and zn (ii) complexes have been synthesized, characterized and screened for their in vitro antibacterial activity against mycobacterium tuberculosis, escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae, bacillus cereus, corynebac ...200616570512
antibiotic susceptibility tests directly on urine samples using uro-quick, a rapid automated system.during the period june 2003-march 2004, 12,579 urine samples were examined employing the uro-quick system. positive samples (1,948) were subsequently gram-stained and processed by standard procedures for microorganism identification and antibiotic susceptibility determination by the disk diffusion method. results of this latter test were compared with those obtained employing the new rapid uro-quick method. antibiotics were introduced in vials containing 2 ml of mueller-hinton broth, then 0.5 ml ...200616572888
modulation of crystalline proteus mirabilis biofilm development on urinary catheters.the crystalline biofilms formed by proteus mirabilis can seriously complicate the care of patients undergoing long-term bladder catheterization. the generation of alkaline urine by the bacterial urease causes calcium and magnesium phosphates to precipitate from urine and accumulate in the catheter biofilm, blocking the flow of urine from the bladder. the ph at which these salts crystallize from a urine sample, the nucleation ph (ph(n)), can be elevated by diluting the urine and by increasing its ...200616585633
clonal occurrences of multidrug-resistant gram-negative bacilli: report from the meropenem yearly susceptibility test information collection surveillance program in the united states (2004).the meropenem yearly susceptibility test information collection program is a global, longitudinal resistance surveillance network of more than 100 medical centers worldwide monitoring the susceptibility of bacterial pathogens to carbapenems and other broad-spectrum agents. between 1999 and 2004, a total of 10-16 us medical centers referred up to 200 nonduplicate isolates from clinical infections to a central processing laboratory. over this 6-year period, the antimicrobial activity of 12 broad-s ...200616466890
antibiotic treatment and resistance of unselected uropathogens in the elderly.a population-based study was conducted among women over the age of 70 years with complaints of uncomplicated urinary tract infections (utis). the positive predictive value of the nitrite test alone or in combination with the leukocyte esterase test ranged between 83% and 99%. the nitrofurantoin prescription rate decreased whereas fluoroquinolone and amoxicillin/clavulanic acid prescriptions increased with increasing age. the aetiology of infection was age-dependent. escherichia coli was the most ...200616473503
functional grouping based on signatures in protein termini.the two ends of each protein are known as the amino (n-) and carboxyl (c-) termini. short signatures in a protein's termini often carry vital cellular function. no systematic research has been conducted to address the importance of short signatures (3 to 10 amino acids) in protein termini at the proteomic level. specifically, it is unknown whether such signatures are evolutionarily conserved, and if so, whether this conservation confers shared biological functions. current signature detection me ...200616475191
cowden disease and lhermitte-duclos disease: an update. case report and review of the literature.cowden disease is a rare autosomal-dominant phacomatosis and cancer syndrome that is associated with lhermitte-duclos disease (ldd), also called dysplastic cerebellar gangliocytoma.200616459996
cmy-16, a novel acquired ampc-type beta-lactamase of the cmy/lat lineage in multifocal monophyletic isolates of proteus mirabilis from northern italy.we report multifocal detection (four different cities in northern italy) of proteus mirabilis isolates resistant to both oxyimino- and 7-alpha-methoxy-cephalosporins and producing a novel acquired ampc-like beta-lactamase. the enzyme, named cmy-16, is a variant of the cmy/lat lineage, which differs from the closest homologues, cmy-4 and cmy-12, by a single amino acid substitution (a171s or n363s, respectively) and from cmy-2 by two substitutions (a171s and w221r). expression of the cloned bla(cm ...200616436718
mössbauer identification of a protonated ferryl species in catalase from proteus mirabilis: density functional calculations on related models.the proteus mirabilis catalase is one of the most efficient heme-containing catalase and forms a relatively stable compound ii. samples of compound ii were prepared from pmc enriched in (57)fe. for the first time, two different forms of compound ii, namely low ph compound ii (lph ii) (43%) and high ph compound ii (hph ii) (25%), have been characterized by mössbauer spectroscopy at ph 8.3. the ratio lph ii/hph ii increases irreversibly with decreasing ph. the large quadrupole splitting value of l ...200616442627
a new gastrointestinal finding in proteus syndrome: report of a case of multiple colonic hemangiomas.a patient with proteus syndrome presented with lower gastrointestinal bleeding due to multiple colonic hemangiomas, a finding which has not been described previously in this syndrome. the patient was a 20-year-old man with features fulfilling the diagnostic criteria for proteus syndrome. he fulfilled both general criteria (mosaic distribution of the lesions, progressive course and sporadic occurrence) and specific criteria (including epidermal nevus, disproportionate overgrowth of limbs and vasc ...200616445505
factors affecting crystal precipitation from urine in individuals with long-term urinary catheters colonized with urease-positive bacterial species.weekly urinalysis was conducted for 12 weeks on a group of 21 long-term catheter users with confirmed catheter encrustation and urinary tract colonization with urease-positive bacteria, in order to explore the cause of considerable variation in the severity of encrustation between sufferers. the rapidity of catheter blockage correlated significantly with the ph above which crystals precipitated from urine (the nucleation ph) but not the ph of the voided urine itself. linear regression showed the ...200616453146
tryptophanase from proteus vulgaris: the conformational rearrangement in the active site, induced by the mutation of tyrosine 72 to phenylalanine, and its mechanistic consequences.tyr72 is located at the active site of tryptophanase (trpase) from proteus vulgaris. for the wild-type trpase tyr72 might be considered as the general acid catalyst at the stage of elimination of the leaving groups. the replacement of tyr72 by phe leads to a decrease in activity for l-tryptophan by 50,000-fold and to a considerable rearrangement of the active site of trpase. this rearrangement leads to an increase of room around the alpha-c atom of any bound amino acid, such that covalent bindin ...200616455316
isolation of salmonella bacteriophages from swine effluent lagoons.bacteriophages (phages) associated with salmonella were collected from nine swine manure lagoons in mississippi. phages were isolated by an enrichment protocol or directly from effluent. for enrichment, chloroform-treated samples were filtered (0.22 mum) and selectively enriched by adding a cocktail of salmonella strains in trypticase soy broth. after overnight incubation at 35 degrees c, chloroform was added and samples stored at 5 degrees c. enriched samples were tested by double agar layer (d ...200616455853
use of electrochemical dna biosensors for rapid molecular identification of uropathogens in clinical urine specimens.we describe the first species-specific detection of bacterial pathogens in human clinical fluid samples using a microfabricated electrochemical sensor array. each of the 16 sensors in the array consisted of three single-layer gold electrodes-working, reference, and auxiliary. each of the working electrodes contained one representative from a library of capture probes, each specific for a clinically relevant bacterial urinary pathogen. the library included probes for escherichia coli, proteus mir ...200616455913
crystallization and preliminary x-ray diffraction analysis of a cold-adapted catalase from vibrio salmonicida.catalase (ec 1.11.1.6) catalyses the breakdown of hydrogen peroxide to water and molecular oxygen. recombinant vibrio salmonicida catalase (vsc) possesses typical cold-adapted features, with higher catalytic efficiency, lower thermal stability and a lower temperature optimum than its mesophilic counterpart from proteus mirabilis. crystals of vsc were produced by the hanging-drop vapour-diffusion method using ammonium sulfate as precipitant. the crystals belong to the monoclinic space group p2(1) ...200616511268
gastric microbiota in elderly patients fed via nasogastric tubes for prolonged periods.aspiration of oropharyngeal contents is a constant threat for elderly patients fed via a nasogastric tube (ngt). colonization of the oropharynx of these patients by pathological flora and the development of biofilms on the feeding tube has been documented recently. in addition, the presence of the ngt may interfere with the gastro-oesophageal sphincter, leading to gastro-oesophageal reflux. thus, the passage of pathogenic bacteria in both directions is facilitated. the purpose of this study was ...200616516342
evaluation of the phoenix 100 id/ast system and nid panel for identification of enterobacteriaceae, vibrionaceae, and commonly isolated nonenteric gram-negative bacilli.the phoenix 100 id/ast system (becton dickinson co., sparks, md.) is an automated system for the identification and antimicrobial susceptibility testing of bacterial isolates. this system with its negative identification (nid) panel was evaluated for its accuracy in the identification of 507 isolates of the family enterobacteriaceae, 57 other nonenteric gram-negative isolates that are commonly isolated in clinical microbiology laboratories, and 138 isolates of the family vibrionaceae. all of the ...200616517878
the potential use of antibacterial peptide antibody indices in the diagnosis of rheumatoid arthritis and ankylosing spondylitis.both rheumatoid arthritis (ra) and ankylosing spondylitis (as) are potentially disabling arthritic disorders for which as yet no highly sensitive and reliable diagnostic laboratory markers are available.200616484874
detection of diverse bacterial signatures in atherosclerotic lesions of patients with coronary heart disease.bacterial infection has been discussed as a potential etiologic factor in the pathophysiology of coronary heart disease (chd). this study analyzes molecular phylogenies to systematically explore the presence, frequency, and diversity of bacteria in atherosclerotic lesions in patients with chd.200616490835
bacteria and the gastrointestinal tract: beneficial and harmful effects.bacteria are unique microorganisms that have a variety of physiological functions which are beneficial to human beings. however, bacteria can also be harmful and cause infections if translocated from the gastrointestinal tract to the epithelial tissue following surgery. the process of translocation can lead to surgical wounds becoming contaminated with gastrointestinal microbes such as escherichia coli, proteus or klebsiella species. bacteria can also cause severe gastrointestinal infections as ...200616493321
[antimicrobial susceptibility of aerobic bacteria isolated from leishmaniotic ulcers in corte de pedra, ba].a prospective study regarding aspects of antimicrobial susceptibility aspects was realized among patients with tegumentary leishmaniasis in corte de pedra, bahia. cases were composed mainly of adolescent and adult farmer patients with single lesions. staphylococcus aureus predominated (83%) in the cultures with susceptibility to the majority of antibiotics. a mixed bacterial flora in ulcers was encountered in 37 (44.1%) patients. among the gram-negative bacteria isolated, enterobacter sp (13.1%) ...200616501766
choice of antibiotic for empirical therapy of acute cystitis in a setting of high antimicrobial resistance.a high prevalence of antimicrobial resistance among urinary isolates in the garhwal region of uttaranchal.200616505574
optimization of ferrioxamine e concentration as effective supplementation for selective isolation of salmonella enteritidis in egg white.utilization of ferrioxamine e (fe) as a sole source of iron distinguishes salmonella from a number of related species, including escherichia coli. fe is not able to serve as a source of iron for e. coli or the proteus-providencia-morganella group. this confers a selective advantage on salmonella enteritidis in egg white supplemented with fe. the optimum concentration of fe that promoted a selective advantage for salmonella in egg white was determined. four supplementation concentrations were eva ...200616541696
isolation of enterobacter sakazakii from stable flies, stomoxys calcitrans l. (diptera: muscidae).enterobacter sakazakii is an opportunistic foodborne pathogen that causes meningitis, enterocolitis, and sepsis, primarily in immunocompromised infants. previously, it was suggested that stable flies, stomoxys calcitrans, were a vector or reservoir of this pathogen. in our study, by means of a culturing approach combined with 16s rdna pcr-restriction fragment length polymorphism genotyping and sequencing, we screened 928 individual stable flies collected in kansas and florida. two stable flies ( ...200616541702
increasing trend in the prevalence of plasmid-mediated ampc beta-lactamases in enterobacteriaceae lacking chromosomal ampc gene at a korean university hospital from 2002 to 2004.the aim of the study is to investigate the prevalence of plasmid-mediated ampc beta-lactamases in enterobacteriaceae naturally lacking chromosomal ampc beta-lactamases. a total of 1860 clinical isolates of klebsiella spp., salmonella spp., and proteus mirabilis were collected from a korean hospital between january 2002 and december 2004. for the isolates that are nonsusceptible to cefoxitin, polymerase chain reaction amplification of the bla(shv), bla(tem), and bla(ampc) genes and sequencing wer ...200616545935
effect of steroid on mitochondrial oxidative stress enzymes, intestinal microflora, and bacterial translocation in rats subjected to temporary liver inflow occlusion.protective effects of steroids against ischemia-reperfusion (i/r) injury are well known, but there is little information about the influence of temporary inflow occlusion on intestinal barrier function or bacterial translocation. the aim of this experimental study was to investigate the effects on liver, kidney, spleen, ileal mitochondrial stress enzymes, and bacterial translocation of methylprednisolone (mp) in rats undergoing temporary liver inflow occlusion. twenty-seven pathogen-free wistar ...200616549125
bioactive components of the traditionally used mushroom podaxis pistillaris.in the course of an ethnobotanical study on fungi used in yemeni ethnomedicine the fungus podaxis pistillaris (podaxales, podaxaceae, basidiomycetes) was found to exhibit antibacterial activity against staphylococcus aureus, micrococcus flavus, bacillus subtilis, proteus mirabilis, serratia marcescens and escherichia coli. in the culture medium of p. pistillaris three epidithiodiketopiperazines were identified by activity-guided isolation. based on spectral data (nmr, esi-ms and dci-ms) their id ...200616550228
the presence of a conjugative gram-positive tn2009 in gram-negative commensal bacteria.to determine whether mef(a)-msr(d) and tet(m) genes are linked in representative gram-negative isolates and/or transferred together during conjugation. to molecularly characterize the acinetobacter junii element and compare the structure and sequence with the non-conjugative streptococcus pneumoniae tn2009 element.200616551693
urinary tract infections in general practice patients: diagnostic tests versus bacteriological culture.urinary tract infections (utis) are common bacterial infections encountered in general practice. for the optimal treatment the general practitioner (gp) should rely on the results of diagnostic tests and recent antimicrobial susceptibility of uropathogens.200616554337
proteus syndrome with huge tonsillar mass causing dysphagia: a rare case.proteus syndrome is a rare and highly variable hamartomatous syndrome that can affect multiple organ systems. it is characterized by hyperplasia of connective tissue, vascular malformations, epidermal naevi and hyperostosis. most of the cases present to plastic and orthopaedic surgeons. otolaryngologic presentations are minimal. we report such a rare case, which presented with a huge unilateral tonsillar mass causing dysphagia.200616556346
the challenges of proteus syndrome: diagnosis and management.proteus syndrome (ps) is a disorder of patchy or mosaic postnatal overgrowth of unknown etiology. the onset of overgrowth typically occurs in infancy and can involve any tissue of the body. commonly involved tissues include connective tissue and bone, skin, central nervous system, the eye, but it apparently can affect any tissue. diagnosing of ps is difficult and the diagnostic criteria are controversial. our group advocates stringent diagnostic criteria to facilitate research and appropriate cl ...200616883308
[acute abdomen secondary to spontaneous uterine rupture associated with pyometra].a 71-year-old female with rheumatoid arthritis and chronic use of corticosteroids presented to the emergency room with 2 weeks of urinary symptoms, abdominal pain and a mass located in hypo-mesogastrium and both flanks. an x-ray film of the abdomen showed that bowels were displaced by the mass. laboratory studies showed thrombocytosis (549,000/mm(3)) and leukocytosis (41,800/mm(3)). several hours after her arrival the patient developed acute abdomen and surgery was indicated. a urinary catheter ...200616887087
altered postnatal lung development in c3h/hej mice.c3h/hej mice develop an increase in terminal air space area detectable by postnatal d 14 that persists into adulthood compared with strain-matched controls (c3h/snj, c3h/ouj). morphometric quantification revealed a 50% increase in terminal air space area by postnatal d 14 and a 2.3-fold increase by 2 mo of age in c3h/hej mice. bacteriologic cultures obtained from the left lung on postnatal d 7 revealed > 100 colony-forming units (cfu)/left lung of predominantly gram-negative bacteria (gnb) (esch ...200617065580
[rapid detection of pseudomonas aeruginosa by the fluorescence quantitative pcr assay targeting 16s rdna].the 16s rdna specific primers were designed for rapid detection of pseudomonas aeruginosa (pa) by the fluorescence quantitative pcr (fq-pcr) assay, based upon multiple sequence alignment and phylogenetic tree analysis of the 16s rdnas of over 20 bacteria. after extraction of pa genomic dna, the target 16s rdna fragment was amplified by pcr with specific primers, and used to construct recombinant pmdt-pfr plasmid, the dilution gradients of which were subjected to the standard quantitation curve i ...200617037203
antimicrobial properties of erythromycin and colistin impregnated bone cement. an in vitro analysis.deep surgical site infection is a devastating consequence of total joint arthroplasty. the use of antibiotic impregnated bone cement is a well-accepted adjunct for treatment of established infection and prevention of deep orthopaedic infection. it allows local delivery of the antibiotic at the cement-bone interface and sustained release of antibiotic provides adequate antibiotic coverage after the wound closure. preclinical testing, randomised and clinical trials indicate that the use of antibio ...200617042224
antimicrobial activity of some ethnomedicinal plants used by paliyar tribe from tamil nadu, india.antimicrobial activity of 18 ethnomedicinal plant extracts were evaluated against nine bacterial strains (bacillus subtilis, staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, escherichia coli, klebsiella pneumonia, pseudomonas aeruginosa, ervinia sp, proteus vulgaris) and one fungal strain (candida albicans). the collected ethnomedicinal plants were used in folk medicine in the treatment of skin diseases, venereal diseases, respiratory problems and nervous disorders.200617042964
bactericidal activity of ertapenem against major intra-abdominal pathogens.treatment of intra-abdominal infections remains a challenge owing to their polymicrobial nature and associated mortality risk. treatment regimens must provide broad-spectrum coverage, including gram-positive and gram-negative aerobic and anaerobic bacteria of gastrointestinal origin. ertapenem is a long-acting 1-beta-methyl parenteral group 1 carbapenem antibiotic that has a broad antibacterial spectrum and once-daily dosing supported by clinical studies. it is active against gram-positive and g ...200617045463
[characteristics of war wound infection].war wounds are the most complex type of non-targeted injuries due to uncontrolled tissue damage of varied and multifold localizations, exposing sterile body areas to contamination with a huge amount of bacteria. wound contamination is caused by both the host microflora and exogenous agents from the environment (bullets, cloth fragments, dust, dirt, water) due to destruction of the host protective barriers. war wounds are the consequence of destructive effects of various types of projectiles, whi ...200617048790
[development of beta-lactamase resistance in enterobacteria].enterobacteria produce elementary chromosomal enzymes, beta-lactamases of class a: tem and shv (escherichia coli, klebsiella pneumoniae). these can give rise to plasmid-coded broad-spectrum beta-lactamases (esbl) discovered in 1980 (e. coli, k. pneumoniae, enterobacter cloacae). the first cefotaximase (ctx-m, men-1) was reported in europe in 1990. this enzyme is far more active against cefotaxime than against ceftazidime and aztreonam. chromosomal hyperpoduction of k1 beta-lactamase differs from ...200617051471
human gamma delta t cells and tumor immunotherapy.human vgamma2vdelta2 t cells recognize nonpeptide antigens derived from pathogenic microbes in a tcr-dependent manner, such as pyrophosphomonoester compounds from mycobacteria and malaria parasite and alkyl amines from proteus, suggesting that this subset of gamma delta t cells is involved in infectious immunity. the precise recognition mechanism has been delineated using a site-directed mutagenesis strategy based on crystal structure of gamma delta tcr. on the other hand, several lines of evide ...200617058804
metalloantibiotics: synthesis, characterization and in-vitro antibacterial studies on cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes with cloxacillin.the synthesis and characterization of cloxacillin (clox) complexes with divalent metal ions [co (ii), cu (ii), ni (ii) and zn (ii)] is described. the nature of bonding of the chelated cloxacillin and the structures of the metal complexes have been elucidated on the basis of their physical and spectroscopic data. in all the complexes, the cloxacillin acts as a uninegatively charged bidentate ligand with coordination involving the carboxylate-o and endocyclic-n of the beta-lactam ring. the new com ...200617059178
[case no. 6. primary abscess of the right psoas-iliac compartment caused by proteus mirabilis in a female patient with nf1]. 200616858877
cysteine based novel noncompetitive inhibitors of urease(s)--distinctive inhibition susceptibility of microbial and plant ureases.based on the catalysis mechanism of urease, a homologous series of 10 cysteine derivatives (cysds) was designed and synthesized, and their inhibitory activities were evaluated for microbial ureases (bacillus pasteurii, bpu, and proteus mirabilis, pmu) and for a plant urease [jack bean (cavavalia ensiformis), jbu]. as already described, thiol-compounds might inhibit urease activity by chelating the nickel atoms involved in the catalysis process. in contrast to cysteine, which has been reported to ...200616859909
structures and serology of the o-antigens of proteus strains classified into serogroup o17 and former serogroup o35.bacteria of the genus proteus are facultative pathogens which commonly cause urinary tract infections. based on the serological specificity of the o-chain polysaccharide of the lipopolysaccharide (o-polysaccharide, o-antigen), strains of p. mirabilis and p. vulgaris have been classified into 60 serogroups. studies on the chemical structure and serological specificity of the o-antigens aim at the elucidation of the molecular basis and improvement of the serological classification of these bacteri ...200616868723
antimicrobial activity of extracts of the lichen xanthoparmelia pokornyi and its gyrophoric and stenosporic acid constituents.the antimicrobial activity of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen xanthoparmelia pokornyi and its gyrophoric acid and stenosporic acid constituents has been screened against some foodborne bacteria and fungi. both the extracts and the acids showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, staphylococcus aureus, streptococcus faecalis, yersinia enterocol ...200616869486
role of type ii topoisomerase mutations and acrab efflux pump in fluoroquinolone-resistant clinical isolates of proteus mirabilis.we conducted a study to determine the role played by amino acid mutations in dna gyrase and topoisomerase iv, and the acrab efflux pump in resistance to fluoroquinolones in clinical isolates of proteus mirabilis.200616870650
systemic absorption of gentamicin nasal irrigations.to determine if gentamicin nasal irrigation is systemically absorbed, and to identify any ototoxic side effects related to its use.200616871924
hemihyperplasia syndromes.hemihyperplasia is a heterogenous group of disorders characterized by asymmetric limb growth. there is considerable confusion regarding their classification and ascertainment into various syndromes. we tried to look into the various aspects of hemihyperplasia syndromes.200616877856
in vitro activities of three carbapenems against recent bacterial isolates from severely ill patients at swedish hospitals.to study the in vitro activity of imipenem, meropenem and ertapenem against common pathogens isolated from patients in intensive care, haematology and dialysis/nephrology units at 7 swedish university hospitals, a total of 788 isolates were collected during 2002-2003. the distribution of the isolates was as follows: escherichia coli (n = 140), klebsiella spp. (n = 132), proteus spp. (n = 97), enterobacter spp. (n = 113), pseudomonas aeruginosa (n = 126), acinetobacter spp. (n = 53) and enterococ ...200617008228
urinary bactericidal activity of oral antibiotics against common urinary tract pathogens in an ex vivo model.in this investigation, the urine samples obtained in a single oral-dose pharmacokinetic study were examined for their bactericidal activity against a range of relevant urinary tract pathogens.200617008781
etiologic agents of cervicovaginitis in turkish women.to investigate the distribution of microbiologic agents causing cervicovaginitis.200617013472
a clinical assessment of the performance of a sensor to detect crystalline biofilm formation on indwelling bladder catheters.to test the ability of a sensor developed to signal infection by the organisms that generate the crystalline biofilms that encrust catheters, to give an early warning that encrustation was occurring on patients' catheters, as the care of many patients undergoing long-term bladder catheterization is complicated by the encrustation and blockage of their catheters.200617026594
[antimicrobial susceptibility of proteus mirabilis urinary tract isolates from 1999 to 2005 at nîmes university hospital].to analyse antimicrobial resistance trends of proteus mirabilis urinary tract isolates from 1999 to 2005 at the nîmes university hospital.200617030456
mutation-positive and mutation-negative patients with cowden and bannayan-riley-ruvalcaba syndromes associated with distinct 10q haplotypes.phosphatase and tensin homolog deleted on chromosome 10 (pten) encodes a tumor-suppressor phosphatase frequently mutated in both sporadic and heritable forms of human cancer. germline mutations are associated with a number of heritable cancer syndromes that are jointly referred to as the "pten hamartoma tumor syndrome" (phts) and include cowden syndrome, bannayan-riley-ruvalcaba syndrome, proteus syndrome, and proteus-like syndrome. germline pten mutations have been identified in a significant p ...200617033968
proteus syndrome with agenesis of the rectus abdominis. 200617034561
[heat shock proteins of freshwater protists and their involvement in adaptation to changes in the environmental salinity].changes in the level of heat shock proteins (hsp) in cells of freshwater protists, amoebae amoeba proteus and ciliates paramecium jenningsi, in response to changes in the environmental salinity were investigated. changes in salinity levels were considered as a stress factor. the immunoblotting method revealed a polypeptide antigen cross-reacting with antibodies against bovine hsp70 in total protein extracts of both intact cells and cells subjected to salinity stress. the same polypeptide antigen ...200616893060
surgical site infections in day surgery settings.because major complications are rare in day surgery, minor morbidities such as surgical site infections, which become evident only after patient discharge, have a major impact on the perceived quality of care. we performed an audit of such infections over a oneyear period.200616895493
new trends in prosthesis infection in cardiovascular surgery.adult cardiovascular surgery entails, in many cases, the use of some kind of prosthesis. among the potential complications, prosthetic device infection is one of the most devastating in incidence, as well as in prognosis and damage to surrounding tissues.200616895504
a case of proteus syndrome (elephant man). 200616898038
[microorganisms isolated from middle ear cultures and their antibacterial susceptibility in patients with chronic suppurative otitis media].this study sought to determine causative microorganisms of chronic suppurative otitis media and their antibacterial susceptibility.200616905907
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2004). i. susceptibility distribution].the bacterial strains isolated from 490 patients diagnosed as having urinary tract infections (utis) in 14 institutions in japan were collected between august 2004 and july 2005. the susceptibilities of them to many kinds of antimicrobial agents were measured. of them, 577 strains were estimated as causative bacteria and used for the measurement. the strains consisted of 156 gram-positive bacterial strains (27.0%) and 421 gram-negative bacterial strains (73.0%). against staphylococcus aureus, ar ...200616913404
how long do nosocomial pathogens persist on inanimate surfaces? a systematic review.inanimate surfaces have often been described as the source for outbreaks of nosocomial infections. the aim of this review is to summarize data on the persistence of different nosocomial pathogens on inanimate surfaces.200616914034
pcr for the identification and differentiation of histomonas meleagridis, tetratrichomonas gallinarum and blastocystis spp.in the present investigation pcr assays were developed for the rapid detection and differentiation of two poultry flagellates: histomonas meleagridis and tetratrichomonas gallinarum as well as the protozoan microorganism: blastocystis spp. the nucleotide sequences of the small subunit ribosomal rnas were used for primer construction obtaining fragments which vary in size for each microorganism. the established pcrs were able to detect dna obtained from one microorganism of t. gallinarum and blas ...200616920265
a comparison of antimicrobial susceptibility profile of urinary pathogens for the years, 1999 and 2003.urinary tract infection is a common condition worldwide; responsible for significant morbidity in both hospitalized and community patients. the laboratory records, for microbial isolates of infected urine and their susceptibility profiles for the years 1999 and 2003 were retrospectively reviewed and compared. in 2003, there was a significant decline in recovery ofcitrobacter spp compared to 1999. conversely, the proportion of k pneumoniae, e coli and enterococci increased dramatically in 2003, i ...200616921702
fishmeal extract bile salt lactose agar--a differential medium for enteric bacteria.fishmeal extract bile salt lactose agar (febla), a new differential medium for enteric bacteria was developed and evaluated for its ability to grow and differentiate lactose fermenters (lf) from non-lactose fermenters (nlf) in comparison with macconkeys agar. performance of febla was at par with the latter. on febla medium, the contrast between lf and nlf colonies was pronounced and klebsiella pneumoniae produced more mucoid colonies than on macconkeys agar (hi media). unlike macconkeys agar, a ...200616924840
emerging resistance to carbapenems in a tertiary care hospital in north india.carbapenems are beta-lactam antibiotics, presently considered as most potent agents for treating multi-drug resistant gram-negative bacilli infections. in india carbapenems available for use are meropenem and imipenem, introduced recently. resistance to these has been reported in a few bacteria especially pseudomonas spp. we therefore retrospectively evaluated the antibiotic susceptibility pattern to these agents amongst various clinical isolates in a tertiary care hospital in north india.200616926463
extended-spectrum beta-lactamases in taiwan: epidemiology, detection, treatment and infection control.extended-spectrum beta-lactamases (esbls) efficiently hydrolyze extended-spectrum beta-lactams such as cefotaxime, ceftriaxone, ceftazidime, and aztreonam. esbls are most often plasmid-mediated. in taiwan, the prevalence of esbls in bacteria has risen, ranging from 8.5 to 29.8% in klebsiella pneumoniae and 1.5 to 16.7% in escherichia coli isolates. the most prevalent types of esbls are shv-5, shv-12, ctx-m-3, and ctx-m-14 in isolates of k. pneumoniae and e. coli, with differences between institu ...200616926972
1h nmr spectroscopy in the diagnosis of klebsiella pneumoniae-induced urinary tract infection.the (1)h nmr spectroscopic method is suggested and its utility is demonstrated for the diagnosis of klebsiella pneumoniae (k. pneumoniae) in urinary tract infection (uti). k. pneumoniae have the specific property of metabolizing glycerol to 1,3-propanediol (1,3-pd), acetate, ethanol and succinate. the quantity of 1,3-pd produced correlates well with the viable bacterial count. other common bacteria causing uti (except for citrobacter frundii), such as escherichia coli (e. coli), pseudomonas aeru ...200616927393
comparison of susceptibility to antimicrobials of bacterial isolates from companion animals in a veterinary diagnostic laboratory in canada between 2 time points 10 years apart.the susceptibility to antimicrobials of bacterial species most frequently isolated from companion animals in a veterinary teaching diagnostic laboratory was evaluated retrospectively. a significant decrease between 1990-1992 and 2002-2003 was noted in the susceptibility of dog isolates to the following antimicrobials: escherichia coli to cephalothin (86% to 61%, p < 0.001); e. coli to ampicillin (85% to 67%, p < 0.001); proteus spp. to ampicillin (92% to 71%, p < 0.01); coagulase-positive staphy ...200616933555
assessment of pathogen frequency and resistance patterns among pediatric patient isolates: report from the 2004 sentry antimicrobial surveillance program on 3 continents.selecting empiric or directed therapy for pathogens isolated from pediatric patients can be problematic. many antimicrobial agents are not indicated for use in pediatric patients, and regional variations of resistance mechanisms have been reported. the purpose of this study was to analyze antimicrobial resistance patterns and pathogen occurrence rates in pediatric-aged patient infections on 3 continents using data from the sentry antimicrobial surveillance program. a total of 3537 clinical isola ...200616938419
effective prevention of microbial biofilm formation on medical devices by low-energy surface acoustic waves.low-energy surface acoustic waves generated from electrically activated piezo elements are shown to effectively prevent microbial biofilm formation on indwelling medical devices. the development of biofilms by four different bacteria and candida species is prevented when such elastic waves with amplitudes in the nanometer range are applied. acoustic-wave-activated foley catheters have all their surfaces vibrating with longitudinal and transversal dispersion vectors homogeneously surrounding the ...200616940055
mode of transposition and expression of 16s rrna methyltransferase gene rmtc accompanied by isecp1.a newly identified 16s rrna methyltransferase gene, rmtc, was accompanied by an isecp1 element at its 5' end. this isecp1 element, which contained a transposase gene, tnpa, provided a promoter activity for expression of the adjacent rmtc; and this structure enabled the rmtc gene to be transposed onto another plasmid in escherichia coli.200616940134
relevance of weil-felix test in diagnosis of scrub typhus in india.to study the relationship of weil-felix test and microimmunofluorescence test.200616941792
unusual fallopian tube neoplasm in a 10-year-old patient with proteus syndrome: a case report and review of the literature.we report a case of fallopian tube and peritoneal lesions resected from a 10-year-old girl with proteus syndrome. the papillary fallopian tube tumor expressed ca-125 and demonstrated negative staining for cea, cd15, calretinin, and ck 5/6. nondilated portions of fallopian tube exhibited evidence of an in situ component. this patient was also found to have peritoneal mesothelial hyperplasia as well as cystic peritoneal lesions of mesothelial origin, with positive calretinin staining. this case pr ...200616944982
visuomotor tracking related to attention-deficit hyperactivity disorder (adhd).deficient visuomotor tracking in children with attention-deficit hyperactivity disorder (adhd) has been described, but the specific influence of attention on this deficit has not yet been elucidated. the present study compares visuomotor tracking under different conditions of attentional loading in children with adhd with that of age-matched controls. a computerized visuomotor attentional tracking test that incorporated several levels of distraction was administered to 13 typical children. the s ...200616948935
the dps gene of symbiotic "candidatus legionella jeonii" in amoeba proteus responds to hydrogen peroxide and phagocytosis.to survive in host cells, intracellular pathogens or symbiotic bacteria require protective mechanisms to overcome the oxidative stress generated by phagocytic activities of the host. by genomic library tagging, we cloned a dps (stands for dna-binding protein from starved cells) gene of the symbiotic "candidatus legionella jeonii" organism (called the x bacterium) (dps(x)) that grows in amoeba proteus. the gene encodes a 17-kda protein (pi 5.19) with 91% homology to dps and dna-binding ferritin-l ...200616950918
a female with hemihypertrophy and chylous ascites - klippel-trenaunay syndrome or proteus syndrome: a diagnostic dilemma.asymmetric overgrowth has many differential diagnoses with considerable overlap, posing a diagnostic dilemma. the presence of chylous ascites, though unreported, might be expected as a manifestation of overgrowth syndromes with lymphatic involvement. we present a patient with hemihypertrophy who presented with chylous ascites at birth.200616957479
analysis on antimicrobial resistance of clinical bacteria isolated from county hospitals and a teaching hospital.the distinction of antimicrobial resistance of clinical bacteria isolated from county hospitals and a teaching hospital was investigated. disc diffusion test was used to study the antimicrobial resistance of isolates collected from county hospitals and a teaching hospital. the data was analyzed by whonet5 and spss statistic software. a total of 655 strains and 1682 strains were collected from county hospitals and a teaching hospital, respectively, in the year of 2003. the top ten pathogens were ...200616961303
occurrence of qnra-positive clinical isolates in french teaching hospitals during 2002-2005.bacteria harbouring the novel qnra plasmid-mediated mechanism of quinolone resistance have been described in different countries, but the frequency of their occurrence has not been investigated. in total, 1,468 clinical isolates of enterobacteriaceae with quinolone resistance or extended-spectrum beta-lactamase (esbl) phenotypes were collected from eight teaching hospitals in france during 2002-2005 and screened for qnra. overall, 28 isolates (22 enterobacter cloacae, three klebsiella pneumoniae ...200616961639
the catalytic machinery of chondroitinase abc i utilizes a calcium coordination strategy to optimally process dermatan sulfate.the chondroitinases are bacterial lyases that specifically cleave chondroitin sulfate and/or dermatan sulfate glycosaminoglycans. one of these enzymes, chondroitinase abc i from proteus vulgaris, has the broadest substrate specificity and has been widely used to depolymerize these glycosaminoglycans. biochemical and structural studies to investigate the active site of chondroitinase abc i have provided important insights into the catalytic amino acids. in this study, we demonstrate that calcium, ...200616964974
synthesis, structure and biological activity of cobalt(ii) and copper(ii) complexes of valine-derived schiff bases.we have synthesized two cobalt(ii) 2 and copper(ii) 3 complexes of valine-derived schiff bases. the obtained complexes were characterized by elemental analysis, ft-ir and x-ray diffraction. biological studies of complexes 2 and 3 had been carried out in vitro for antimicrobial activity against gram-positive, gram-negative bacteria and human pathogenic fungi. compound 3 was proven to be a broad spectrum agent, showed a significant inhibition of the growth of gram-positive bacteria (staphylococcus ...200616965817
ft-ir microspectroscopy for early identification of some clinically relevant pathogens.to investigate the potentials and limitations of fourier transform-infrared (ft-ir) microspectroscopy as a tool to identify, at the level of microcolonies, pathogenic bacteria frequently isolated in the clinical environment.200616968290
a novel gene involved in regulating the flagellar gene cascade in proteus mirabilis.in this study, we identified a transposon insertion in a novel gene, designated disa, that restored swarming motility to a putrescine-deficient spea mutant of proteus mirabilis. a null allele in disa also increased swarming in a wild-type background. the disa gene product was homologous to amino acid decarboxylases, and its role in regulating swarming was investigated by examining the expression of genes in the flagellar cascade. in a disa mutant background, we observed a 1.4-fold increase in th ...200616980463
[evaluation of anti-blastoma effects of tetravaccine in combination with chemotherapy].the present work discusses methods of immune-correction and immune-rehabilitation of oncological patients using specific bacterial polyvalent vaccines. with this purpose the tetra-vaccine (staphilococcus-proteus-clepsiela-escherichia) has been used. experiments have been carried out on white rats and mice with sarcoma c-45. for chemotherapy of malignant tumors the following remedies were used: cyclophosphan, vincristin and adriablastin. the vaccine and chemotherapy were applied in both ways, sep ...200616980764
urinary bactericidal activity of extended-release ciprofloxacin (1,000 milligrams) versus levofloxacin (500 milligrams) in healthy volunteers receiving a single oral dose.twelve volunteers received a single oral dose of 1,000 mg extended-release (xr) ciprofloxacin versus 500 mg levofloxacin to assess urinary bactericidal titers (ubts) against common uropathogens. areas under ubt-time curves were significantly larger for proteus mirabilis with xr ciprofloxacin and for staphylococci with levofloxacin.200616982785
rapid onset of ulcerative typhlocolitis in b6.129p2-il10tm1cgn (il-10-/-) mice infected with helicobacter trogontum is associated with decreased colonization by altered schaedler's flora.infection with helicobacter trogontum, a urease-positive helicobacter isolated from subclinically infected rats, was evaluated in b6.129p2-il10(tm1cgn) (interleukin-10(-/-) [il-10(-/-)]) and c57bl/6 (b6) mice. in a first experiment, il-10(-/-) mice naturally infected with helicobacter rodentium had subclinical typhlocolitis but developed severe diarrhea and loss of body condition with erosive to ulcerative typhlocolitis within 1 to 3 weeks of experimental infection with h. trogontum. a second ex ...200616982822
multiple bacterial species reside in chronic wounds: a longitudinal study.the aim of the study was to investigate the bacterial profile of chronic venous leg ulcers and the importance of the profile to ulcer development. patients with persisting venous leg ulcers were included and followed for 8 weeks. every second week, ulcer samples were collected and the bacterial species present were identified. more than one bacterial species were detected in all the ulcers. the most common bacteria found were staphylococcus aureus (found in 93.5% of the ulcers), enterococcus fae ...200616984578
[development of a gold-immunochromatography test for rapid detecting e. coli o157].to develop a method for rapid detecting escherichia coli (e. coli) o157 on site.200616986519
antimicrobial activity of extracts of chemical races of the lichen pseudevernia furfuracea and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents.the antimicrobial activity and the mic values of the ethanol, chloroform, diethyl ether, and acetone extracts of the chemical races of pseudevernia furfuracea (var. furfuracea and var. ceratea) and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents have been investigated against some microorganisms. nearly all extracts of both chemical races showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, prote ...200616989308
diabetic foot ulcers in cameroon: can microflora prevalence inform probabilistic antibiotic treatment?to determine the clinical features, regularly associated microorganisms and their susceptibility to antibiotics, and the clinical outcomes of foot ulcers in patients with diabetes at the yaoundé central hospital, cameroon.200617001946
phytochemical and antimicrobial study of an antidiabetic plant: scoparia dulcis l.the antimicrobial and antifungal effects of different concentrations of chloroform/methanol fractions of scoparia dulcis were investigated. the isolated fractions were tested against different bacteria like salmonella typhii, staphylococcus aureus, escherichia coli, bacillus subtilis, pseudomonas aeruginosa, and proteus vulgaris and fungal strains such as alternaria macrospora, candida albicans, aspergillus niger, and fusarium oxysporum. the isolated fractions exhibited significant antimicrobial ...200617004904
inhibition of swarming and virulence factor expression in proteus mirabilis by resveratrol.resveratrol (3,5,4-trihydroxy-trans-stilbene) is a phytoalexin compound with anti-inflammatory and antioxidant activities. the effect of resveratrol on swarming and virulence factor expression of proteus mirabilis, an important pathogen infecting the urinary tract, was determined on swarming agar plates with and without the compound. bacteria harvested at different times were assayed for cell length and the production of flagella, haemolysin and urease. resveratrol inhibited p. mirabilis swarmin ...200617005777
identification and quantitation of usnic acid from the lichen usnea species of anatolia and antimicrobial activity.six species of lichens, such as usnea florida, usnea barbata, usnea longissima, usnea rigida, usnea hirta and usnea subflorida, were collected from different areas of anatolia (district of antalya, karabük, qankiri, giresun and trabzon) in turkey. their usnic acid amounts in acetone extracts were determined by hplc. in addition, antimicrobial activities of these extracts were determined against escherichia coli (atcc 35218), enterococcus faecalis (rskk 508), proteus mirabilis (pasteur ens. 235), ...200617294685
calcium sulphate delivery system with tobramycin for the treatment of chronic calcaneal osteomyelitis.the case of a 24-year-old woman with pseudomonas aeruginosa and proteus mirabilis chronic calcaneal osteomyelitis is presented. extensive debridement of the necrotic bone and application of tobramycin-loaded polymethylmethacrylate beads was performed and ciprofloxacin was given post-operatively. three months later, laboratory tests, including complete blood cell count, erythrocyte sedimentation rate and c-reactive protein, were normal. at this time, extensive surgical debridement and filling of ...200617295005
[antagonistic effect of bacteriocinogenic lactobacillus acidophilus on klebsiella pneumoniae, citrobacter freundii and proteus mirabilis cells].data of the ultrastructural cellular changes of conditionally pathogenic enterobacteria, including k. pneumoniae, c. freundii and p. mirabilis cells impacts to bacteriocin-producing l. acidophilus are presented. enterobacteria in response to the bacteriocinogenic effect of lactobacilli are manifestated by expressive destructions of sensitive to pore formation bacteriocin cells. various morphological types of enterobacteria cells with increase of involution, lysing and resting forms are revealed. ...200617297872
[pyrazinamidase activity of bacteria from enterobacteriaceae family as characteristic for taxonomy].pyrazinamidase activity in 330 strains of bacteria from enterobacteriaceae family (14 genus, 27 species) has been assessed. pyrazinamidase activity detected in species from following genuses: citrobacter, escherichia, klebsiella, kluyvera, morganella, providencia, raourtella, salmonella, shigella, and also in proteus mirabilis, and nonpathogenic serovars of yersinia enterocolitica, y. frederiksenii. pirasinamidase was absent in serratia (s. marcescens, s. liguefaciens), hafnia alvei, p. vulgaris ...200617297889
more than just a bump: the hamartoma syndromes.the hamartoma syndromes detailed in this review are just a few examples of the many genodermatoses now known to be associated with uncontrolled tumor proliferation secondary to mutations in tumor suppressor genes. knowledge gained through the study of these syndromes has not only improved our understanding of patients afflicted with such conditions, but has also led to significant insight into the important role tumor suppressor genes play in preventing tumor formation and in carcinogenesis. as ...200617249301
[bacteriological evaluation in chronic otitis media].in chronic otitis media bacterial infection is often a cause of exacerbation in the clinical course and failure in treatment. the aim of the study was to investigate the incidence and profile of the aerobic bacterial flora in chronic otitis media and to determine the drug susceptibility of selected isolated bacteria.200617263251
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