Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| assessment of correlation between clinical parameters and pulp canal pathogens in endodontic pathologies: a microbiological study. | success and failure of root canal therapy is largely dependent on the clinician and the patient. once the tooth is restored into its functional and esthetic place, the root canal therapy is considered to be complete. it is a well-known fact that root canal space acts as a rich reservoir for microorganisms. past studies show that root canal harbors an array of different microorganisms. streptococcus mitis and enterococcus faecalis have been found to be the most prevalent microorganisms isolated f ... | 2016 | 27659083 |
| antibacterial activity of leaf extract of annona muricata and simarouba glauca on enterococcus faecalis. | to determine the antimicrobial effect of water extracts of leaves of annona muricata and simarouba glauca on enterococcus faecalis using agar diffusion method. | 2016 | 27659082 |
| an invitro evaluation of antimicrobial efficacy and flow characteristics for ah plus, mta fillapex, crcs and gutta flow 2 root canal sealer. | cleaning and shaping of root canal does not guarantee complete disinfection of the canal, regardless of the systems and techniques used for this purpose. therefore, it becomes mandatory for the endodontic filling materials to have a good antimicrobial potential especially against e. faecalis. this research was aimed therefore to evaluate the antibacterial efficacy and flow properties of different root canal sealers. | 2016 | 27656550 |
| effect of bacteremia in elderly patients with urinary tract infection. | the clinical effect of bacteremia on outcomes in urinary tract infection (uti) is still debated. this study aims to examine the clinical effect of bacteremia in elderly patients with uti requiring hospital admission. | 2016 | 27650231 |
| prevalence of virulence factors and vancomycin-resistant genes among enterococcus faecalis and e. faecium isolated from clinical specimens. | the aim of this study was to determine the occurrence of virulence determinants and vancomycin-resistant genes among enterococcus faecalis and e. faecium obtained from various clinical sources. | 2016 | 27648425 |
| nationwide surveillance of novel oxazolidinone resistance gene optra in enterococcus isolates in china from 2004 to 2014. | a total of 2,201 nonduplicate enterococcal isolates collected from 29 hospitals in 23 cities in china between 2004 and 2014 were screened for the oxazolidinone resistance gene optra; 45 isolates (2.0%) were optra positive with 11 optra variants identified. the positive rate of optra increased from 0.4% to 3.9% during the 10-year surveillance period. pulsed-field gel electrophoresis (pfge) and multilocus sequence type (mlst) analysis revealed that 37 optra-positive enterococcus faecalis isolates ... | 2016 | 27645239 |
| role of interspecies interactions in dual-species biofilms developed in vitro by uropathogens isolated from polymicrobial urinary catheter-associated bacteriuria. | most catheter-associated urinary tract infections are polymicrobial. here, uropathogen interactions in dual-species biofilms were studied. the dual-species associations selected based on their prevalence in clinical settings were klebsiella pneumoniae-escherichia coli, e. coli-enterococcus faecalis, k. pneumoniae-e. faecalis, and k. pneumoniae-proteus mirabilis. all species developed single-species biofilms in artificial urine. the ability of k. pneumoniae to form biofilms was not affected by e. ... | 2016 | 27642801 |
| the in vitro effect of antimicrobial photodynamic therapy with indocyanine green on enterococcus faecalis: influence of a washing vs non-washing procedure. | the purpose of this study was to evaluate the in vitro effect of washing and non-washing of indocyanine green (icg) as photosensitizer (ps) on bacterial count, biofilm formation, development and degradation of enterococcus faecalis. | 2016 | 27640733 |
| phage therapy against enterococcus faecalis in dental root canals. | antibiotic resistance is an ever-growing problem faced by all major sectors of health care, including dentistry. recurrent infections related to multidrug-resistant bacteria such as methicillin-resistant staphylococcus aureus, carbapenem-resistant enterobacteriaceae, and vancomycin-resistant enterococci (vre) in hospitals are untreatable and question the effectiveness of notable drugs. two major reasons for these recurrent infections are acquired antibiotic resistance genes and biofilm formation ... | 2016 | 27640530 |
| vana-targeted oligonucleotide dna probe designed to monitor vancomycin- and teicoplanin-resistant bacteria in surface waters. | the glycopeptide antibiotics teicoplanin and vancomycin are common to treat severe gram-positive bacterial infections. the gene vana confers high-level resistance to these antibiotics, and these phenomena have been shown to be transferable. release of vancomycin- and teicoplanin-resistant bacteria to surface waters is, therefore, of particular concern since they might proliferate and spread in different environments. monitoring of the fate of vana gene in these waters provides information on the ... | 2016 | 27640164 |
| time-dependent antibacterial effects of aloe vera and zataria multiflora plant essential oils compared to calcium hydroxide in teeth infected with enterococcus faecalis. | in the present in vitro study, we investigated the time-related antimicrobial efficacy of aloe vera and zataria multiflora (z. multiflora) plant essential oils compared to calcium hydroxide ([ca[oh]2 ) to eliminate enterococcus faecalis (e. faecalis) from root canals. | 2016 | 25187255 |
| efficacy of low concentrations of sodium hypochlorite and low-powered er,cr:ysgg laser activated irrigation against an enterococcus faecalis biofilm. | to establish the antibacterial efficacy of low concentrations of sodium hypochlorite with and without er,cr:ysgg laser activation on enterococcus faecalis biofilms in extracted teeth. | 2016 | 25772335 |
| daptomycin versus vancomycin in an enterococcus faecalis endophthalmitis rabbit model. | to compare bactericidal activities of daptomycin (dap) and vancomycin (van) in an experimental rabbit model of enterococcus faecalis endophthalmitis. | 2016 | 25658242 |
| antimicrobial activity of different disinfection methods against biofilms in root canals. | the aim of the present study was to evaluate the bactericidal effects of different disinfection methods against microbial biofilms grown in root canals. | 2016 | 25641829 |
| manual and rotary instrumentation ability to reduce enterococcus faecalis associated with photodynamic therapy in deciduous molars. | this aim of this study was to assess the ability of manual or rotary instrumentation associated with photodynamic therapy (pdt) to reduce enterococcus faecalis using three combinations of light/photosensitizers: toluidine blue o/laser, fuchsin/halogen light and fuchsin/led. twenty deciduous molars were selected and contaminated with enterococcus faecalis (mcfarland 0.5 scale). working length determination was performed by visual method. the teeth were randomly divided into two groups: g1 (n=10): ... | 2016 | 25590196 |
| effect of a surfactant on the antimicrobial activity of sodium hypochlorite solutions. | the objective of the present study was to evaluate the antimicrobial activity of sodium hypochlorite (naocl) associated with a surfactant. seventy single-rooted extracted human teeth were inoculated with enterococcus faecalis, and incubated for 21 days (37 °c). the groups were distributed according to the irrigation solution used during root canal preparation: 5%, 2.5% and 1% naocl; 5%, 2.5% and 1% hypoclean®, a solution containing a surfactant (cetrimide) associated with naocl. three microbiolo ... | 2016 | 25517777 |
| mechanical reduction of the intracanal enterococcus faecalis population by hyflex cm, k3xf, protaper next, and two manual instrument systems: an in vitro comparative study. | in the present study, the effectiveness of three rotary and two manual nickel titanium instrument systems on mechanical reduction of the intracanal enterococcus faecalis population was evaluated. | 2016 | 25424649 |
| assessing outcomes of adult oncology patients treated with linezolid versus daptomycin for bacteremia due to vancomycin-resistant enterococcus. | the incidence and severity of vancomycin-resistant enterococcus blood stream infections continue to rise and is a significant burden in the healthcare setting. literature thus far is minimal regarding treatment outcomes in patients with malignancy and vancomycin-resistant enterococcus bacteremia. appropriate antibiotic selection is vital to treatment success due to high rates of resistance, limited antimicrobials and mortality in this patient population. we conducted this study to determine whet ... | 2016 | 25326010 |
| confocal microscopy evaluation of the effect of irrigants on enterococcus faecalis biofilm: an in vitro study. | the purpose of this study was to evaluate in vitro the effectiveness of two endodontic irrigants and their association against enterococcus faecalis (e. faecalis) by confocal laser scanning microscope (clsm). twenty-four bovine incisors were inoculated in a monoculture of e. faecalis for 21 days. after this period, the teeth were divided into three test groups (n = 5) according to the chemical used. group 1: 2.5% sodium hypochlorite (naocl), group 2: 2% chlorhexidine gel (chx), group 3: 2.5% nao ... | 2016 | 26153228 |
| transcription factor rex in regulation of pathophysiology in oral pathogens. | the nad(+) and nadh-sensing transcriptional regulator rex is widely conserved across gram-positive bacteria. rex monitors cellular redox poise and controls the expression of genes/operons involved in diverse pathways including alternative fermentation, oxidative stress responses, and biofilm formation. the oral cavity undergoes frequent and drastic fluctuations in nutrient availability, ph, temperature, oxygen tension, saliva, and shear forces. the oral streptococci are major colonizers of oral ... | 2016 | 26172563 |
| antimicrobial efficacy of photodynamic therapy, nd:yag laser and qmix solution against enterococcus faecalis biofilm. | lasers have been recommended in final root canal disinfection protocol, however, there is no clear evidence about their efficacy against bacteria in biofilms. the aim of the study was to evaluate and compare the disinfection effect of antimicrobial photodynamic therapy (apdt), nd:yag laser and qmix solution against enterococcus faecalis biofilm. | 2016 | 26232719 |
| combined effects of photodynamic therapy and irrigants in disinfection of root canals. | in this study, the combined effects of photodynamic therapy and irrigants in eradicating common endodontic pathogens are evaluated. roots of 80 extracted single rooted teeth are divided into 2 groups (1) mechanical flushing; (2) antibacterial irrigation. after cleaning and shaping, they are inoculated with either (a) streptococcus mutans or (b) enterococcus faecalis and incubated. they are again subdivided and either only irrigated or irrigated and lased. dentin shavings are taken from root cana ... | 2016 | 26235897 |
| investigation of mechanisms and molecular epidemiology of linezolid nonsusceptible enterococcus faecalis isolated from a teaching hospital in china. | the epidemiological and molecular characteristics of eight linezolid nonsusceptible enterococcus faecalis isolated from a teaching hospital in china (january to july 2014) were investigated. the target site modifications and cfr gene associated with linezolid resistance were not found. results of the epidemiological investigation indicated that linezolid resistance possibly occurred on several independent occasions and was often not related to linezolid administration. | 2016 | 26210761 |
| a comparison of the antibacterial activity of the two methods of photodynamic therapy (using diode laser 810 nm and led lamp 630 nm) against enterococcus faecalis in extracted human anterior teeth. | failure of endodontic treatment is usually due to an inadequate disinfection of the root canal system. enterococcus faecalis has been widely used as a valuable microbiological marker for in-vitro studies because of its ability to colonize in a biofilm like style in root canals, invading dentinal tubules and resistance to some endodontic treatments. the aim of this study was to investigate the antibacterial effects of two methods of photodynamic therapy using a light emitting diode lamp (led lamp ... | 2016 | 26241781 |
| sealing ability of two root-end filling materials in a bacterial nutrient leakage model. | to compare in vitro the sealing ability of root-end fillings with mineral trioxide aggregate (mta) and endosequence bioceramic root repair material-fast set (bc-rrm) putty using a novel bacterial nutrient leakage model, which provides information on whether or not intracanal bacteria are receiving nutrients from serum via leakage channels. | 2016 | 26334201 |
| low level of resistance in enterococci isolated in four hospitals, marseille, france. | enterococci are gram-positive cocci responsible for various infections worldwide, and their prevalence of antibiotic resistance greatly varies worldwide. this study investigates the prevalence of resistance to antibiotics in enterococci from patients admitted in the four university hospitals of marseille between january 2013 and september 2014. two thousand nine hundred seventy-six patients-bacteria couples were identified (2,507 enterococcus faecalis and 469 enterococcus faecium) in the four un ... | 2016 | 26247097 |
| enterococcus faecalis endophthalmitis as a metastatic complication of hemodialysis vascular access-related sepsis: a case report and review of the literature. | catheter and/or arteriovenous (a-v) graft-related bacteremia is an important cause of morbidity and mortality among hemodialysis (hd) patients. endocarditis, septic arthritis, epidural abscess, septic embolism, and osteomyelitis are the most common complications of catheter and/or a-v graft-related bacteremia; however, endogenous endophthalmitis is rarely seen. to the best of our knowledge, enterococcus faecalis is the first case report in this population. we hereby report a case of endogenous e ... | 2016 | 26346615 |
| how long can culturable bacteria and total dna persist in environmental waters? the role of sunlight and solid particles. | in this work, sunlight inactivation of two indicator bacteria in freshwater, with and without solid particles, was studied and the persistence of culturable cells and total dna was compared. environmental water was used to prepare two matrices, with and without solid particles, which were spiked with escherichia coli and enterococcus faecalis. these matrices were used to prepare microcosm bags that were placed in two containers: one exposed to sunlight and the other in the dark. during one month ... | 2016 | 26379262 |
| lethal bacterial trap: cationic surface for endodontic sealing. | insoluble antibacterial cationic nanoparticles have been previously shown to have potent and long-lasting antibacterial properties. our tested hypothesis was that root canal pathogens will be attracted to and eliminated when exposed to epoxy resin-based surfaces incorporating cationic nanoparticles. in our research, an epoxy resin-based surface incorporating quaternary ammonium polyethyleneimine (qpei) nanoparticles was evaluated. surface characterization was performed using atomic force microsc ... | 2016 | 26418438 |
| evaluation of tedizolid against staphylococcus aureus and enterococci with reduced susceptibility to vancomycin, daptomycin or linezolid. | tedizolid displays potent activity against gram-positive pathogens. in vitro studies against clinical isolates of staphylococcus aureus and enterococci demonstrated improved tedizolid activity over linezolid. however, this is not well-characterized against a large collection of resistant isolates, including vancomycin-intermediate s. aureus (visa), heterogeneous visa (hvisa), daptomycin-non-susceptible (dns) s. aureus, linezolid-resistant (lr) s. aureus and vre. therefore, our objective was to d ... | 2016 | 26476277 |
| covalent immobilization of enterococcus faecalis esawy dextransucrase and dextran synthesis. | enterococcus faecalis esawy dextransucrase was immobilized in fe(3+)-cross-linked alginate/carboxymethyl cellulose (ac) beads. the gel beads were modified with polyethylenimine (pei) followed by glutaraldehyde (ga) to form fe(3+) (acpg) beads. fe(3+) (acpg) was characterized using ftir and dsc techniques. ga activated beads showed new two peaks. the first was at 1,717 cm(-1) which refers to (co) group of a free aldehyde end of glutaraldehyde, and another peak was at 1,660 cm(-1) referring to (cn ... | 2016 | 26434519 |
| virulence of bacteria colonizing vascular bundles in ischemic lower limbs. | we documented previously the presence of bacterial flora in vascular bundles, lymphatics, and lymph nodes of ischemic lower limbs amputated because of multifocal atheromatic changes that made them unsuitable for reconstructive surgery and discussed their potential role in tissue destruction. the question arose why bacterial strains inhabiting lower limb skin and considered to be saprophytes become pathogenic once they colonize deep tissues. bacterial pathogenicity is evoked by activation of mult ... | 2016 | 26431369 |
| relative contribution of biological variation and technical variables to zone diameter variations of disc diffusion susceptibility testing. | disc diffusion is still largely based on manual procedures. technical variations originate from inoculum preparation, variations in materials, individual operator plate streaking and reading accuracy. resulting measurement imprecision contributes to categorization errors. biological variation resembles the natural fluctuation of a measured parameter such as antibiotic susceptibility around a mean value. it is deemed to originate from factors such as genetic background or metabolic state. this st ... | 2016 | 26462987 |
| retrospective study for risk factors for febrile uti in spinal cord injury patients with routine concomitant intermittent catheterization in outpatient settings. | retrospective study. | 2016 | 26458969 |
| mature biofilms of enterococcus faecalis and enterococcus faecium are highly resistant to antibiotics. | enterococcus faecalis and enterococcus faecium are important nosocomial pathogens that form biofilms on implanted materials. we compare the antibiotic sensitivity of bacteria in new (established during 24 hours) and mature (established during 120 hours) enterococcal biofilms. mature biofilms contained more bacteria and were much more tolerant to antibiotics, including rifampicin-containing combinations, as judged by determination of minimal biofilm eradication concentrations and by time-kill exp ... | 2016 | 26458279 |
| chitosan nanoparticles enhance the antibacterial activity of chlorhexidine in collagen membranes used for periapical guided tissue regeneration. | endodontic failure is mainly associated with the persistence of microbial infection in the root canal system and/or the periradicular area. microorganisms and their toxins located in the root canal system may trigger apical periodontitis and tissue destruction. tissue regeneration in periapical surgery by using membrane barriers has shown an improved healing and bone closure. however, bacterial membrane contamination is a main reason of failure. in this in vitro study, different brands of chlorh ... | 2016 | 26478419 |
| high-level aminoglycoside resistance in enterococcus faecalis and enterococcus faecium causing invasive infection: twelve-year surveillance in the minami ibaraki area. | we examined prevalence of high-level aminoglycoside resistance (hlar) in enterococcus faecalis and enterococcus faecium causing invasive infection in the minami ibaraki area. ten strains of both species each, recovered from the blood or the cerebrospinal fluid between 2003 and 2014, were randomly selected every year. high-level resistance to gentamicin (hlr-gm) and streptomycin (hlr-sm) was detected in 34% (41 of 120 strains) and 18% (21) of e. faecalis and 9% (11) and 39% (48) of e. faecium, re ... | 2016 | 26492859 |
| prototype development of the intelligent hydrogel wound dressing and its efficacy in the detection of model pathogenic wound biofilms. | the early detection of wound infection in situ can dramatically improve patient care pathways and clinical outcomes. there is increasing evidence that within an infected wound the main bacterial mode of living is a biofilm: a confluent community of adherent bacteria encased in an extracellular polymeric matrix. here we have reported the development of a prototype wound dressing, which switches on a fluorescent color when in contact with pathogenic wound biofilms. the dressing is made of a hydrat ... | 2016 | 26492095 |
| surface protein esp enhances pro-inflammatory cytokine expression through nf-κb activation during enterococcal infection. | enterococcal surface protein (esp) is encoded on a pathogenicity island in enterococcus faecalis and e. faecium and is involved in biofilm formation and binding to epithelial cells. in this study, using esp-expressing e. faecalis mmh594 and its isogenic esp-deficient strain, as well as purified esp, we show that esp is sufficient for activation of nf-κb and the subsequent production of pro-inflammatory cytokines il-1β and tnf-α in macrophages in vitro. in a mouse peritonitis model, we also show ... | 2016 | 26503704 |
| antimicrobial effect of the extracts from hypericum perforatum against oral bacteria and biofilm formation. | one traditional medicines, hypericum perforatum l. (hypericaceae), possesses several beneficial effects against depression, ulcers, dyspepsia, abdominal pains, burns, bacterial infections, migraine headaches, and sciatica. | 2016 | 26510970 |
| antibacterial effect of n-acetylcysteine and taurolidine on planktonic and biofilm forms of enterococcus faecalis. | this study investigated the antimicrobial activity of taurolidine and n-acetylcysteine (nac) on planktonic and biofilm enterococcus faecalis phenotypes. | 2016 | 26515652 |
| abutment coating with diamond-like carbon films to reduce implant-abutment bacterial leakage. | the influence of diamond-like carbon (dlc) films on bacterial leakage through the interface between abutments and dental implants of external hexagon (eh) and internal hexagon (ih) designs was evaluated. | 2016 | 26513267 |
| the native plasmid pml21 plays a role in stress tolerance in enterococcus faecalis ml21, as analyzed by plasmid curing using plasmid incompatibility. | to investigate the role of the native plasmid pml21 in enterococcus faecalis ml21's response to abiotic stresses, the plasmid pml21 was cured based on the principle of plasmid incompatibility and segregational instability, generating e. faecalis mutant strain ml0. the mutant and the wild strains were exposed to abiotic stresses: bile salts, low ph, h2o2, ethanol, heat, and nacl, and their survival rate was measured. we found that curing of pml21 lead to reduced tolerance to stress in e. faecalis ... | 2016 | 26519343 |
| bioconversion of methanol to value-added mevalonate by engineered methylobacterium extorquens am1 containing an optimized mevalonate pathway. | methylotrophic biosynthesis using methanol as a feedstock is a promising and attractive method to solve the over-dependence of the bioindustry on sugar feedstocks derived from grains that are used for food. in this study, we introduced and engineered the mevalonate pathway into methylobacterium extorquens am1 to achieve high mevalonate production from methanol, which could be a platform for terpenoid synthesis. we first constructed a natural operon (mve) harboring the mvas and mvae genes from en ... | 2016 | 26521242 |
| daptomycin antibiotic lock therapy for hemodialysis patients with gram-positive bloodstream infections following use of tunneled, cuffed hemodialysis catheters: retrospective single center analysis. | catheter-related blood stream infection (crbsi) is a major complication in hemodialysis patients. we assessed the efficacy of systemic daptomycin (dpt) plus dpt antibiotic lock therapy (dpt-alt) for catheter salvage in patients with gram-positive crbsis. this is a retrospective study of hemodialysis patients with tunneled and cuffed hemodialysis catheters. all patients were from a single institution in taipei and received systemic dpt plus dpt-alt for the treatment of gram-positive crbsi. succes ... | 2016 | 26549513 |
| implant placement in failed endodontic sites: a review. | dental implants may fail to osseointegrate in sites of endodontic failure. this may occur as a result colonization by various anaerobic and facultative bacterial species. if an implant is placed in a site where vegetative bacteria are residing, the implant may fail to integrate if a bacterial colonization proceeds coronally. if the implant apical cortical bone is thin or if there is an apical fenestration, the colonization may proceed through the thin or nonexistent bone through the covering muc ... | 2016 | 26550925 |
| effect of a benzalkonium chloride surfactant-sodium hypochlorite combination on elimination of enterococcus faecalis. | the purpose of this investigation was to determine the effect of a sodium hypochlorite-surfactant combination on the removal of enterococcus faecalis from infected teeth. | 2016 | 26577873 |
| effect of antimicrobials used in regenerative endodontic procedures on 3-week-old enterococcus faecalis biofilm. | we evaluated the effect of various antimicrobials used in endodontic regeneration on a 3-week-old enterococcus faecalis biofilm. | 2016 | 26577870 |
| influence of biological fluids in bacterial viability on different hospital surfaces and fomites. | the hospital environment is susceptible to bacterial contamination along with survival in fomites and surfaces, allowing dissemination of potential pathogenic strains. the present research aimed to evaluate the influence of biological fluids in bacterial viability on fomites and surfaces commonly present in nosocomial environment. | 2016 | 26577628 |
| enterococcus faecalis attenuates osteogenesis through activation of p38 and erk1/2 pathways in mc3t3-e1 cells. | to explore the role of enterococcus faecalis in the proliferation, apoptosis and differentiation of osteoblasts. | 2016 | 26572053 |
| 1h, 15n and 13c chemical shift assignment of the gram-positive conjugative transfer protein trahpip501. | conjugative transfer of dna represents the most important transmission pathway in terms of antibiotic resistance and virulence gene dissemination among bacteria. trah is a putative transfer protein of the type iv secretion system (t4ss) encoded by the gram-positive (g+) conjugative plasmid pip501. this molecular machine involves a multi-protein core complex spanning the bacterial envelope thereby serving as a macromolecular secretion channel. here, we report the near complete (1)h, (13)c and (15 ... | 2016 | 26559076 |
| establishment of a multi-species biofilm model to evaluate chlorhexidine efficacy. | chronic infections, for example, diabetic foot ulcers, have a large impact in terms of patient morbidity and mortality. these wounds are characterized by complex polymicrobial communities of bacteria, which may include a number of difficult-to-eradicate multidrug-resistant pathogens. | 2016 | 26597632 |
| screening for antimicrobial and proteolytic activities of lactic acid bacteria isolated from cow, buffalo and goat milk and cheeses marketed in the southeast region of brazil. | lactic acid bacteria (lab) can be isolated from different sources such as milk and cheese, and the lipolytic, proteolytic and glycolytic enzymes of lab are important in cheese preservation and in flavour production. moreover, lab produce several antimicrobial compounds which make these bacteria interesting for food biopreservation. these characteristics stimulate the search of new strains with technological potential. from 156 milk and cheese samples from cow, buffalo and goat, 815 isolates were ... | 2016 | 26608755 |
| virulence factors, antimicrobial resistance pattern and molecular analysis of enterococcal strains isolated from burn patients. | the enterococci are emerging as a significant cause of hospital acquired infections. the pathogenesis of enterococci is attributed to the production of virulence factors and resistance to antibiotics. the purpose of the study was to assess the prevalence of genes encoding virulence factor, antimicrobial resistance determinant and molecular characteristic of enterococci isolated from burn patients. a total of 57 enterococci isolated from wound specimens of patients with burn injury were character ... | 2016 | 26620079 |
| on-chip isothermal nucleic acid amplification on flow-based chemiluminescence microarray analysis platform for the detection of viruses and bacteria. | this work presents an on-chip isothermal nucleic acid amplification test (inaat) for the multiplex amplification and detection of viral and bacterial dna by a flow-based chemiluminescence microarray. in a principle study, on-chip recombinase polymerase amplification (rpa) on defined spots of a dna microarray was used to spatially separate the amplification reaction of dna from two viruses (human adenovirus 41, phi x 174) and the bacterium enterococcus faecalis, which are relevant for water hygie ... | 2016 | 26624222 |
| the origin of endodontic enterococcus faecalis explored by comparison of virulence factor patterns and antibiotic resistance to that of isolates from stool samples, blood cultures and food. | to elucidate the origin of enterococcus faecalis isolated from secondary root canal infections and the possibility for a foodborne transmission by comparing them to strains recovered from food, blood and stool regarding putative virulence factors and antibiotic susceptibility profiles, where strains from common origin were hypothesized to harbour similar characteristics. | 2016 | 25950381 |
| l-lactic acid production from glycerol coupled with acetic acid metabolism by enterococcus faecalis without carbon loss. | glycerol is a by-product in the biodiesel production process and considered as one of the prospective carbon sources for microbial fermentation including lactic acid fermentation, which has received considerable interest due to its potential application. enterococcus faecalis isolated in our laboratory produced optically pure l-lactic acid from glycerol in the presence of acetic acid. gas chromatography-mass spectrometry analysis using [1, 2-(13)c2] acetic acid proved that the e. faecalis strain ... | 2016 | 26168904 |
| obturation techniques allow microbial leakage unless protected. | to evaluate the quality of the apical 5-mm seal produced by different filling techniques using a bacterial leakage model. | 2016 | 25996203 |
| collagen-like peptide sequences inhibit bacterial invasion of root dentine. | to investigate the effects of peptides derived from the sequence of collagen to inhibit penetration of human or bovine dentine by species of streptococci and enterococci. | 2016 | 26011655 |
| telavancin activity tested against gram-positive clinical isolates from european, russian and israeli hospitals (2011-2013) using a revised broth microdilution testing method: redefining the baseline activity of telavancin. | to reassess the activity of telavancin when tested against gram-positive clinical pathogens recovered from hospitalized patients in european and adjacent regions using a revised broth microdilution method. | 2016 | 26058844 |
| biological evaluation and molecular modelling study of thiosemicarbazide derivatives as bacterial type iia topoisomerases inhibitors. | in the present article, we describe the inhibitory potency of nine thiosemicarbazide derivatives against bacterial type iia topoisomerases, their antibacterial profile and molecular modelling evaluation. we found that one of the tested compounds, compound 7, significantly inhibits activity of staphylococcus aureus dna gyrase with an ic(50) below 15 μm. besides, this compound displays antibacterial activity on reference staphylococuss spp. and enterococcus faecalis strains as well as clinical s. ... | 2016 | 25792505 |
| antimicrobial 2-hydroxyisocaproic acid and chlorhexidine resist inactivation by dentine. | to compare the antibacterial activity of 2-hydroxyisocaproic acid (hica) with currently used root canal medicaments and to examine their interactions with potential inhibitors in nutrient-deficient and nutrient-rich conditions. | 2016 | 25944560 |
| pharmacokinetics of enrofloxacin and ceftiofur in plasma, interstitial fluid, and gastrointestinal tract of calves after subcutaneous injection, and bactericidal impacts on representative enteric bacteria. | this study's objectives were to determine intestinal antimicrobial concentrations in calves administered enrofloxacin or ceftiofur sodium subcutaneously, and their impact on representative enteric bacteria. ultrafiltration devices were implanted in the ileum and colon of 12 steers, which received either enrofloxacin or ceftiofur sodium. samples were collected over 48 h after drug administration for pharmacokinetic/pharmacodynamic analysis. enterococcus faecalis or salmonella enterica (5 × 10(5) ... | 2016 | 25989138 |
| different extracts of zingiber officinale decrease enterococcus faecalis infection in galleria mellonella. | dried, fresh and glycolic extracts of zingiber officinale were obtained to evaluate the action against g. mellonella survival assay against enterococcus faecalis infection. eighty larvae were divided into: 1) e. faecalis suspension (control); 2) e. faecalis + fresh extract of z. officinale (feo); 3) e. faecalis + dried extract of z. officinale (deo); 4) e. faecalis + glycolic extract of z. officinale (geo); 5) phosphate buffered saline (pbs). for control group, a 5 μl inoculum of standardized su ... | 2016 | 25831098 |
| purification and characterization of a bacteriocin from an oenological strain of leuconostoc mesenteroides subsp. cremoris. | malolactic fermentation (mlf), which improves organoleptic properties and biologic stability of some wines, may cause wine spoilage if uncontrolled. bacteriocins were reported as efficient preservatives to control mlf through their bactericidal effect on malolactic bacteria. leuconostoc mesenteroides subsp. cremoris w3 isolated from wine produces an inhibitory substance that is bactericidal against malolactic bacteria in model wine medium. treatment of the culture supernatant of strain w3 with p ... | 2016 | 25837975 |
| phenolic content, antibacterial and antioxidant activities of erica herbacea l. | antibacterial and antioxidant activity, total phenolic and flavonoid concentrations of aqueous, ethanol and ethyl acetate extracts from the leaves and flowers of erica herbacea l. were studied. in vitro antibacterial activity of the extracts was determined by macrodilution method. minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) have been determined. testing was performed on 30 clinical isolates, including different strains of escherichia coli, enterococcus fae ... | 2016 | 24383325 |
| [multivalvular infective endocarditis with enterococcus faecalis]. | infective endocarditis (ie) is a relatively rare disease that can take part of sepsis and may be a diagnostic and therapeutic challenge. polyvalvular location of el is rare. we present a patient of 60 years diagnosed with plurivalvular infective endocarditis by enterococcus faecalis with septic complications, visceral, dragged, hospitalized for a psychiatric pathology, who raised the problems of diagnosis and treatment, without being able to make assessments on the entrance gate. for help in dia ... | 2016 | 23077930 |
| microbiology of root canal infections. | inflammatory reaction in the periapical tissues is induced by microbial infection in the root canal system. the aim of root canal treatment is to preserve healthy periapical tissues or to provide healing of them in restorable teeth, which have sufficient periodontal support. the amount of microbial cells in the root canal system and their virulence as well as host responses influence on the degree of periapical inflammation and symptoms. microbial biofilm formation is typically seen on root cana ... | 2016 | 28826437 |
| synthesis and characterization of new chlorhexidine-containing nanoparticles for root canal disinfection. | root canal system disinfection is limited due to anatomical complexities. better delivery systems of antimicrobial agents are needed to ensure efficient bacteria eradication. the purpose of this study was to design chlorhexidine-containing nanoparticles that could steadily release the drug. the drug chlorhexidine was encapsulated in poly(ethylene glycol)-block-poly(l-lactide) (peg-b-pla) to synthesize bilayer nanoparticles. the encapsulation efficiency was determined through thermogravimetric an ... | 2016 | 28773576 |
| the effect of gelatinase production of enterococcus faecalis on adhesion to dentin after irrigation with various endodontic irrigants. | objectives: the aim of this study was to evaluate whether the gelatinase production ability of enterococcus faecalis provides any advantage on adhesion of this bacterium to dentin treated with various irrigants and their combinations. materials and methods: standardized dentin discs were randomly divided into five groups (n = 20): group 1: 2.5% sodium hypochlorite (naocl), group 2: 2% chlorhexidine (chx), group 3: naocl + saline + chx, group 4: naocl + edta + naocl, group 5: qmix. after incubati ... | 2016 | 28642925 |
| diversity of vaginal lactic acid bacterial microbiota in 15 algerian pregnant women with and without bacterial vaginosis by using culture independent method. | bacterial vaginosis (bv) is the most common lower genital tract disorder among women of reproductive age (pregnant and non-pregnant) and a better knowledge of lactobacillus species richness in healthy and infected vaginal microbiota is needed to efficiently design better probiotic products to promote the maintenance of normal flora which will help prevent bacterial vaginosis. | 2016 | 27790434 |
| anti-inflammatory and regenerative effects of albanian propolis in experimental vital amputations. | this study evaluated the effects of albanian propolis on the inflamed pulpal tissue after pulpotomy in piglets. in five piglets, two teeth each were infected using special pathogenic microbial flora that was prepared in advance in order to cause inflammation of the pulp. pulpotomy was performed in the maxillary and mandibular central and lateral incisors. microbial flora pathogenesis prepared from the section of infected teeth, containing staphylococcus aureus, streptococcus viridans, enterococc ... | 2016 | 28509506 |
| in vitro antimicrobial activity screening of xylaria hypoxylon. | fungi have a potential of using both as nutritive and medicinal food stuff. because of containing several therapeutic agents, they are reported to be used for hundreds of years to treat several diseases caused by bacteria, fungi, viruses and parasites. the aim of this study is to determine the in vitro antimicrobial activity of xylaria hypoxylon, which were collected from yomra, trabzon, turkey. | 2016 | 28852719 |
| integrating highly quantitative proteomics and genome-scale metabolic modeling to study ph adaptation in the human pathogen enterococcus faecalis. | genome-scale metabolic models represent the entirety of metabolic reactions of an organism based on the annotation of the respective genome. these models commonly allow all reactions to proceed concurrently, disregarding the fact that at no point all proteins will be present in a cell. the metabolic reaction space can be constrained to a more physiological state using experimentally obtained information on enzyme abundances. however, high-quality, genome-wide protein measurements have been chall ... | 2016 | 28725473 |
| antibacterial effect of azadirachta indica (neem) or curcuma longa (turmeric) against enterococcus faecalis compared with that of 5% sodium hypochlorite or 2% chlorhexidine in vitro. | the purpose of this study was to compare the antibacterial properties of azadirachta indica (neem) or curcuma longa (turmeric) against enterococcus faecalis with those of 5% sodium hypochlorite or 2% chlorhexidine as root canal irrigants in vitro. the activity of neem, chlorhexidine, sodium hypochlorite, or turmeric against e. faecalis was measured on agar plates using the agar diffusion method. the tube dilution method was used to determine the minimum inhibitory concentration (mic) and minimum ... | 2017 | 28724858 |
| biofilm formation in enterococci: genotype-phenotype correlations and inhibition by vancomycin. | enterococci are nosocomial pathogens that can form biofilms, which contribute to their virulence and antibiotic resistance. although many genes involved in biofilm formation have been defined, their distribution among enterococci has not been comprehensively studied on a genome scale, and their diagnostic ability to predict biofilm phenotypes is not fully established. here, we assessed the biofilm-forming ability of 90 enterococcal clinical isolates. major patterns of virulence gene distribution ... | 2017 | 28720810 |
| virulence genes in biofilm producer enterococcus faecalis isolates from root canal infections. | enterococcus faecalis is occurring in opportunistic infections involving the oral cavity. this study aimed to evaluate the presence of e. faecalis virulence genes in dental root canal isolates recovered from advanced chronic periodontitis patients. one hundred e. faecalis isolated from dental root canal during july 2015 to oct 2016. after analysis of biofilm formation by the semi-quantitative determination in 96-well flat bottom polystyrene plates, the presence of asa, esp, efaa, ace, ebpr, gel ... | 2017 | 28719346 |
| screening of oxalate degrading lactic acid bacteria of food origin. | a screening for oxalate degrading abilities was initially carried on within lactic acid bacteria cultures of different food origin. seventy-nine strains were drop-inoculated onto mrs agar plates containing calcium oxalate. by comparing colonies diameters, 31 strains were used to inoculate, in parallel, mrs and mrs modified by sodium oxalate addition. differences in the strains' growth were assessed by colony forming unit counts. for two strains, the growth in oxalate enriched medium was signific ... | 2017 | 28713789 |
| genetic characterisation confirms sporadic occurrence of vancomycin-resistant enterococcus faecalis in copenhagen, denmark. | 2017 | 28709988 | |
| short-term alcohol abstinence improves antibacterial defenses of chronic alcohol-consuming mice against gut bacteria-associated sepsis caused by enterococcus faecalis oral infection. | the effects of short-term alcohol abstinence on host antibacterial resistance against enterococcus faecalis oral infection was investigated in chronic alcohol-consuming mice [mice with 0.1 g/day of 20% ethanol consumption for 12 or 16 weeks (cac-mice)]. these mice were highly susceptible to the infection; however, after 7 days of alcohol abstinence (aacac-mice), their antibacterial resistances were completely restored to the normal mouse level. normal mice inoculated with cac-mouse hepatic macro ... | 2017 | 28708971 |
| virulence and antibiotic resistance of enterococci isolated from healthy breastfed infants. | pathogenic ability has been extensively studied in clinical enterococci, but to a lesser extent in community-derived ones. most studies to date in enterococci from healthy infants have been focused on enterococcus faecalis, despite the growing concern about nosocomial infections caused by e. faecium. in this work, we studied the antibiotic resistance and virulence determinants of 26 e. faecalis and 15 e. faecium intestinal isolates from spanish healthy breastfed infants. overall, commensal enter ... | 2017 | 28708453 |
| evaluation of free or anchored antimicrobial peptides as candidates for the prevention of orthopaedic device-related infections. | the prevention of implant-associated infection, one the most feared complications in orthopaedic surgery, remains a major clinical challenge and urges development of effective methods to prevent bacterial colonization of implanted devices. alpha-helical antimicrobial peptides (amps) may be promising candidates in this respect due to their potent and broad-spectrum antimicrobial activity, their low tendency to elicit resistance and possible retention of efficacy in the immobilized state. the aim ... | 2017 | 28707817 |
| postoperative enterococcus faecalis endophthalmitis: virulence factors leading to poor visual outcome. | purpose postoperative endophthalmitis caused by enterococcus faecalis often results in poor visual outcome due to retinal destruction. we evaluated the virulence factors of enterococcus faecalis clinical isolates and analyzed their relationship with retinal destruction and poor visual outcomes in postoperative bacterial endophthalmitis (pbe) following cataract surgery. | 2017 | 28707018 |
| characterization and application of antilisterial enterocins on model fresh cheese. | enterococcus faecalis strains isolated from an artisanal cheese were selected based on enterocin production against listeria monocytogenes. the strains formed biofilms and presented high hydrophobic character and good autoaggregation and coaggregation capacity with l. monocytogenes. strains l3a21m3 and l3b1k3 presented high survival under gastrointestinal conditions, were able to adhere to human intestinal cells (caco-2 and ht-29), and blocked the adhesion and invasion of l. monocytogenes. the a ... | 2017 | 28703625 |
| restructuring of enterococcus faecalis biofilm architecture in response to antibiotic-induced stress. | bacterial biofilms are intrinsically resistant to antimicrobial treatment, which contributes to microbial persistence in clinical infections. enterococcus faecalis is an opportunistic pathogen that readily forms biofilms and is the most prevalent enterococcal species identified in healthcare-associated infections. since intrinsic resistance to multiple antibiotics is common for enterococci, and antibiotic resistance is elevated in biofilm populations, it is imperative to understand the mechanism ... | 2017 | 28685097 |
| draft genome sequence of enterococcus faecalis atcc baa-2128. | while a part of the native gut microflora, the gram-positive bacterium enterococcus faecalis can lead to serious infections elsewhere in the body. the draft genome of e. faecalis strain atcc baa-2128, isolated from piglet feces, was examined. this draft genome consists of 42 contigs, 12 of which exhibit homology to annotated plasmids. | 2017 | 28684576 |
| role of purified β-1, 3 glucan binding protein (β-gbp) from paratelphusa hydrodromus and their anti-inflammatory, antioxidant and antibiofilm properties. | β- 1, 3-glucan binding protein (β-gbp), a pattern recognition protein (prp), plays a critical role in triggering the innate immune response by detecting β-glucan found on the surface of microbes. in the present study, β-gbp was purified from the haemolymph of rice field crab paratelphusa hydrodromus by affinity column chromatography. the monomeric protein ph-β-gbp appeared as a single band with a molecular weight of approximately 95 kda in sds-page analysis and its purity was determined to be 89 ... | 2017 | 28684323 |
| incidence, clinical characteristics, and outcomes of nosocomial enterococcus spp. bloodstream infections in a tertiary-care hospital in beijing, china: a four-year retrospective study. | enterococcus spp. are the common cause of nosocomial bloodstream infections (bsis) with high morbidity and mortality. the purpose of this study was to characterize the incidence, clinical and microbiological features, and mortality of nosocomial enterococcal bsis at a large chinese tertiary-care hospital in beijing, china. | 2017 | 28680588 |
| the enterococcus faecalis virulence factor elra interacts with the human four-and-a-half lim domains protein 2. | the commensal bacterium enterococcus faecalis is a common cause of nosocomial infections worldwide. the increasing prevalence of multi-antibiotic resistant e. faecalis strains reinforces this public health concern. despite numerous studies highlighting several pathology-related genetic traits, the molecular mechanisms of e. faecalis virulence remain poorly understood. in this work, we studied 23 bacterial proteins that could be considered as virulence factors or involved in the enterococcus inte ... | 2017 | 28676674 |
| stochasticity in the enterococcal sex pheromone response revealed by quantitative analysis of transcription in single cells. | in enterococcus faecalis, sex pheromone-mediated transfer of antibiotic resistance plasmids can occur under unfavorable conditions, for example, when inducing pheromone concentrations are low and inhibiting pheromone concentrations are high. to better understand this paradox, we adapted fluorescence in situ hybridization chain reaction (hcr) methodology for simultaneous quantification of multiple e. faecalis transcripts at the single cell level. we present direct evidence for variability in the ... | 2017 | 28671948 |
| carriage of multidrug resistant enterococcus faecium and enterococcus faecalis among apparently healthy humans. | enterococci are indigenous flora of the gastro-intestinal tracts of animals and humans. recently, interest in two major species, e. faecium and e. faecalis, has heightened because of their ability to cause serious infections and their intrinsic resistance to antimicrobials. this study was aimed at determining the prevalence of e. faecium and e. faecalis in human faecal samples and evaluating the susceptibility of the isolates to antibiotics. | 2017 | 28670644 |
| prevalence and genetic diversity of enterococcus faecalis isolates from mineral water and spring water in china. | enterococcus faecalis is an important opportunistic pathogen which is frequently detected in mineral water and spring water for human consumption and causes human urinary tract infections, endocarditis and neonatal sepsis. the aim of this study was to determine the prevalence, virulence genes, antimicrobial resistance and genetic diversity of e. faecalis from mineral water and spring water in china. of 314 water samples collected from january 2013 to january 2014, 48 samples (15.3%) were contami ... | 2017 | 28670302 |
| determining the optimal dose of 1940-nm thulium fiber laser for assisting the endodontic treatment. | insufficient cleaning, the complex anatomy of the root canal system, inaccessible accessory canals, and inadequate penetration of irrigants through dentinal tubules minimizes the success of the conventional endodontic treatment. laser-assisted endodontic treatment enhances the quality of conventional treatment, but each laser wavelength has its own its own limitations. the optimal parameters for the antibacterial efficiency of a new wavelength, 1940-nm thulium fiber laser, were firstly investiga ... | 2017 | 28669067 |
| urinary tract infections after kidney transplantation: a risk factor analysis of 417 patients. | background the aim of this study was to evaluate the number of urinary tract infections (uti) that occur after kidney transplantation (kt) and to identify possible risk factors for development of a uti. material and methods we retrospectively analyzed all kts performed between january 2012 and december 2013 in the erasmus university medical center, rotterdam. uti was scored if: (1) a patient had a urine culture with no more than 2 species of microorganisms, (2) at least 1 of which was a bacteriu ... | 2017 | 28663538 |
| altered antibacterial activity of curcumin in the presence of serum albumin, plasma and whole blood. | antibacterial effect is one of the major therapeutic activities of plant-derived curcumin. this work evaluated the effect of serum albumin, human plasma, and whole blood on the in vitro activity of curcumin against eight clinical bacterial isolates by standard broth microdilution and plate-counting methods. toxicological effects of curcumin towards human red blood cells (rbcs) and peripheral blood mononuclear cells (pbmcs) were also investigated. curcumin exhibited weak activity against gram-neg ... | 2017 | 28649069 |
| [mitral valve endocarditis diagnosed after panophthalmitis with enterococcus faecalis]. | an 86-year-old man underwent cataract surgery on the left eye, but suddenly he lost his vision three days after surgery. topical treatments for panophthalmitis were started according to international guidelines. later, a broad-range polymerase chain reaction showed enterococcus faecalis in corpus vitreum, and iv antiobiotic treatment was initiated. to exclude endocarditis an echocardiography was performed, and this showed mitral regurgitation with a small mobile vegetation. uncertainty concernin ... | 2017 | 28648168 |
| antibacterial effect of genetically-engineered bacteriophage ϕef11/ϕfl1c(δ36)p(nisa) on dentin infected with antibiotic-resistant enterococcus faecalis. | enterococcus faecalis is a gram-positive facultative anaerobic bacterium, which is present in 30-89% of teeth with postendodontic treatment failures. e. faecalis is capable of penetrating dentinal tubules and surviving as a monoculture after conventional endodontic therapy, indicating that it is resistant to commonly used endodontic disinfection protocols. different e. faecalis strains have shown resistance to several antibiotics, and have been associated with both dental pathology and systemic ... | 2017 | 28646693 |
| phtβ-promoted mobilization of non-conjugative resistance plasmids from enterococcus faecium to enterococcus faecalis. | to analyse the recombination events associated with conjugal mobilization of two multiresistance plasmids, prum17i48 and plag (formerly named pdo1-like), from enterococcus faecium 17i48 to enterococcus faecalis jh2-2. | 2017 | 28645197 |
| methacrylate-based root canal sealer containing chlorexidine and α-tricalcium phosphate. | the aim of this study was to develop and to characterize a methacrylate-based root canal sealer containing chlorhexidine (chx) and α-tricalcium phosphate (α-tcp). experimental dual cure methacrylate-based sealer was produced containing 0, 2.5, or 5 wt% of chx and 0, 25, or 50 wt% of α-tcp. experimental sealers were evaluated based on flow, film thickness, radiopacity, degree of conversion (dc), degradation in water, ph and antibacterial activity. flow ranged from 15.09 ± 0.11 to 17.47 ± 0.42 mm. ... | 2017 | 28644564 |
| exploiting crispr-cas to manipulate enterococcus faecalis populations. | crispr-cas provides a barrier to horizontal gene transfer in prokaryotes. it was previously observed that functional crispr-cas systems are absent from multidrug-resistant (mdr) enterococcus faecalis, which only possess an orphan crispr locus, termed crispr2, lacking cas genes. here, we investigate how the interplay between crispr-cas genome defense and antibiotic selection for mobile genetic elements shapes in vitro e. faecalis populations. we demonstrate that crispr2 can be reactivated for gen ... | 2017 | 28644125 |